Rh5AG195900

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000865
Physical Location & Seq
Forward (+)
22743548 .. 22790976
47429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG195900.1

Sequence Viewer

Length: 1299 bp
ATGGCTGAACTTGCAATTCCTTTATCATTGTTGGTGGCCGAGAAGGCGATGAGCTTGTGCGACCGAAGAAACAAGTCGGAGGACCCAAGTAATAATATCCATGATGATGTTGAAAGAGCCTGGAAATGTCTAAAGAGGATGCAAGCCTATCTAGGGGACTCGGAAGAGATTGATGAAGGCAGCGGCAGCCTAATGCTTCAAAATCGTGTCGCAGAAGTCCAAAGTCTGGCTTATGAAATAGAGGATGCTCTTGAGAAGTTCATATCTCAGATTCCACATCATTTCCATAGCAACTTATTCTCTCAGAAGCTGCACGACGTTACGCACTATCGCGAAGAAAAGAGAGCAAGAGTTGAGTTATCCACCAAGATTAAAGACATTGAAAAGAAAATTAGTTCCTTCTCAACTTTGGATTCACTTCTTGAGCCACCAAGTAGTTCCAGCACAAGGAAGAGGGATAGTTTGATCCACCAAGTTCTTGAGGAAGATGAAATGGTAGGCCTTGAGGAGCCTAAAGAAAGACTCTCCAAACAGTTGAGGGAAGAAGATTCAAGGCTCAAGACTATTTTGGTTGTAGGTCCTGGCGGCTCCGGCAAAACCACAGTTGTGAAGAATGTTTTTGACAGCACCATGGTCCAAATGTTTTTTGATTGCCATGCTTGGATCGACGTGTCACGGCCTCCTAAAGCAGAAGATCAGAAAGCTGAACTGATGCATCTGAAGGAAAAGAGATTTTTGTTGCCGTTCCGGCTCTGGATTCCCAAGACTCTGAGCACGCTAGCTGGTGCTGTTCTCGGATCGTCGTCTCCGGTGACGCAATTGAAGAGGAAGTGGTCGGCGAAGCTTTGCATTCCGATTGTATGGATGAGATTTGGGGCTGCTCCGGGGACGCCGTCTATGGCGGCATGGAGAGAGGTCGATATTTCTGGGGTTAATGTGGAAAAATTTGATGACAAGTATTTCGGAAAGGCCGTGGAAAAGAAGAAGAAGAAGGGGGAGGGAGAGTTTTTTGAGGCAGAGAAAGAGGACAAGAAAGTGTTCCCACAAGAGAGGAAAGATGATCAGAAGAGTGTGGATGCTTCGTTGATAAAGTCCATTGACGTTGTCCCAGACTTGAAGACATACTTAGCTGCAAGATTCTCTCTGAGGTCTGGCATGTCTTTTAGACAGTCAGCTCTTTTCTTACTTAGGATACTTGTTCCCGTTCTTGTTTGTTTCTCTACTATGATTGTATGTTCTGTTCACATTGAGATCCGAGATTTTGTACTGATCGGCATTGCCTTGCAAGGGATGTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

432

Amino Acids

49.02

Weight (kDa)

7.63

Isoelectric Point (pI)

53.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 31 - 95 2.9e-07 Rx N-terminal domain
NB-ARC PF00931 169 - 230 1.3e-10 NB-ARC domain
Ribosomal_L6e PF01159 304 - 388 1.5e-21 Ribosomal protein L6e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 226
AccII CGCG 1 cut(s) 333
AciI CCGC 3 cut(s) 183, 585, 902
AclWI GGATC 4 cut(s) 460, 671, 805, 1246
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 1 cut(s) 944
AcuI CTGAAG 1 cut(s) 740
AcyI GRCGYC 1 cut(s) 890
AfaI GTAC 1 cut(s) 1266
AfiI CCNNNNNNNGG 4 cut(s) 153, 226, 447, 1287
AflIII ACRYGT 1 cut(s) 669
AgsI TTSAA 6 cut(s) 113, 200, 383, 552, 823, 1117
AjiI CACGTC 1 cut(s) 670
AjnI CCWGG 2 cut(s) 119, 580
AleI CACNNNNGTG 1 cut(s) 605
AluBI AGCT 7 cut(s) 54, 310, 704, 782, 844, 1130, 1175
AluI AGCT 7 cut(s) 54, 310, 704, 782, 844, 1130, 1175
Alw21I GWGCWC 1 cut(s) 776
Alw26I GTCTC 1 cut(s) 810
AlwI GGATC 4 cut(s) 460, 671, 805, 1246
AlwNI CAGNNNCTG 1 cut(s) 310
AoxI GGCC 4 cut(s) 36, 499, 677, 969
ApeKI GCWGC 5 cut(s) 180, 186, 310, 878, 1130
ApoI RAATTY 1 cut(s) 944
ArsI GACNNNNNNTTYG 2 cut(s) 944, 976
Asp700I GAANNNNTTC 1 cut(s) 1037
AspS9I GGNCC 3 cut(s) 82, 578, 634
AsuC2I CCSGG 1 cut(s) 885
AsuHPI GGTGA 1 cut(s) 823
AsuNHI GCTAGC 1 cut(s) 778
AvaII GGWCC 3 cut(s) 82, 578, 634
BbsI GAAGAC 1 cut(s) 1124
Bbv12I GWGCWC 1 cut(s) 776
BbvI GCAGC 5 cut(s) 192, 198, 297, 865, 1117
BceAI ACGGC 4 cut(s) 692, 727, 877, 956
BciT130I CCWGG 2 cut(s) 121, 582
BciVI GTATCC 1 cut(s) 1185
BclI TGATCA 1 cut(s) 1060
BcnI CCSGG 1 cut(s) 885
BcoDI GTCTC 1 cut(s) 810
BfaI CTAG 2 cut(s) 152, 779
BfuI GTATCC 1 cut(s) 1185
BglI GCCNNNNNGGC 2 cut(s) 44, 748
BisI GCNGC 8 cut(s) 181, 184, 187, 311, 586, 879, 903, 1131
BlsI GCNGC 8 cut(s) 182, 185, 188, 312, 587, 880, 904, 1132
Bme1390I CCNGG 3 cut(s) 121, 582, 885
Bme18I GGWCC 3 cut(s) 82, 578, 634
BmgBI CACGTC 1 cut(s) 670
BmgT120I GGNCC 3 cut(s) 82, 578, 634
BmiI GGNNCC 3 cut(s) 84, 510, 589
BmrFI CCNGG 3 cut(s) 121, 582, 885
BmsI GCATC 5 cut(s) 129, 235, 702, 724, 1066
BmtI GCTAGC 1 cut(s) 782
BpiI GAAGAC 1 cut(s) 1124
BpuEI CTTGAG 5 cut(s) 272, 443, 500, 524, 542
BpuMI CCSGG 1 cut(s) 885
BsaHI GRCGYC 1 cut(s) 890
BsaJI CCNNGG 3 cut(s) 630, 884, 972
BsaWI WCCGGW 1 cut(s) 808
BsaXI ACNNNNNCTCC 2 cut(s) 989, 1019
Bsc4I CCNNNNNNNGG 4 cut(s) 153, 226, 447, 1287
Bse3DI GCAATG 1 cut(s) 1275
BseBI CCWGG 2 cut(s) 121, 582
BseDI CCNNGG 3 cut(s) 630, 884, 972
BseGI GGATG 5 cut(s) 144, 250, 870, 1081, 1296
BseLI CCNNNNNNNGG 4 cut(s) 153, 226, 447, 1287
BseMI GCAATG 1 cut(s) 1275
BseMII CTCAG 4 cut(s) 281, 317, 761, 1136
BseRI GAGGAG 1 cut(s) 521
BseXI GCAGC 5 cut(s) 192, 198, 297, 865, 1117
BsgI GTGCAG 1 cut(s) 296
Bsh1236I CGCG 1 cut(s) 333
Bsh1285I CGRYCG 1 cut(s) 64
BshFI GGCC 4 cut(s) 38, 501, 679, 971
BsiEI CGRYCG 1 cut(s) 64
BsiHKAI GWGCWC 1 cut(s) 776
BsiSI CCGG 4 cut(s) 591, 748, 809, 884
BslFI GGGAC 3 cut(s) 170, 901, 1091
BslI CCNNNNNNNGG 4 cut(s) 153, 226, 447, 1287
BsmAI GTCTC 1 cut(s) 810
BsmBI CGTCTC 1 cut(s) 810
BsmFI GGGAC 3 cut(s) 170, 901, 1091
BsmI GAATGC 1 cut(s) 849
BsnI GGCC 4 cut(s) 38, 501, 679, 971
Bsp1286I GDGCHC 1 cut(s) 776
Bsp143I GATC 7 cut(s) 465, 663, 694, 797, 1060, 1251, 1269
Bsp19I CCATGG 1 cut(s) 630
Bsp68I TCGCGA 1 cut(s) 333
BspACI CCGC 3 cut(s) 183, 585, 902
BspANI GGCC 4 cut(s) 38, 501, 679, 971
BspCNI CTCAG 4 cut(s) 280, 316, 762, 1137
BspFNI CGCG 1 cut(s) 333
BspLI GGNNCC 3 cut(s) 84, 510, 589
BspOI GCTAGC 1 cut(s) 782
BspPI GGATC 4 cut(s) 460, 671, 805, 1246
BsrDI GCAATG 1 cut(s) 1275
BssECI CCNNGG 3 cut(s) 630, 884, 972
BssMI GATC 7 cut(s) 465, 663, 694, 797, 1060, 1251, 1269
BssNI GRCGYC 1 cut(s) 890
BssT1I CCWWGG 1 cut(s) 630
Bst2UI CCWGG 2 cut(s) 121, 582
Bst4CI ACNGT 3 cut(s) 534, 604, 1170
Bst6I CTCTTC 4 cut(s) 159, 446, 818, 1061
BstACI GRCGYC 1 cut(s) 890
BstC8I GCNNGC 3 cut(s) 144, 776, 780
BstDEI CTNAG 6 cut(s) 267, 303, 770, 1126, 1145, 1187
BstDSI CCRYGG 2 cut(s) 630, 972
BstF5I GGATG 5 cut(s) 144, 250, 870, 1081, 1296
BstFNI CGCG 1 cut(s) 333
BstKTI GATC 7 cut(s) 468, 666, 697, 800, 1063, 1254, 1272
BstMAI GTCTC 1 cut(s) 810
BstMBI GATC 7 cut(s) 465, 663, 694, 797, 1060, 1251, 1269
BstMCI CGRYCG 1 cut(s) 64
BstMWI GCNNNNNNNGC 5 cut(s) 11, 44, 186, 591, 748
BstNI CCWGG 2 cut(s) 121, 582
BstNSI RCATGY 1 cut(s) 1159
BstSCI CCNGG 3 cut(s) 119, 580, 883
BstUI CGCG 1 cut(s) 333
BstV1I GCAGC 5 cut(s) 192, 198, 297, 865, 1117
BstV2I GAAGAC 1 cut(s) 1124
BstX2I RGATCY 1 cut(s) 1251
BstYI RGATCY 1 cut(s) 1251
BsuI GTATCC 1 cut(s) 1185
BsuRI GGCC 4 cut(s) 38, 501, 679, 971
BtgI CCRYGG 2 cut(s) 630, 972
BtgZI GCGATG 1 cut(s) 62
BtrI CACGTC 1 cut(s) 670
BtsCI GGATG 5 cut(s) 144, 250, 870, 1081, 1296
BtuMI TCGCGA 1 cut(s) 333
Cac8I GCNNGC 3 cut(s) 144, 776, 780
CaiI CAGNNNCTG 1 cut(s) 310
Cfr13I GGNCC 3 cut(s) 82, 578, 634
CseI GACGC 2 cut(s) 823, 898
Csp6I GTAC 1 cut(s) 1265
CviAII CATG 5 cut(s) 101, 631, 656, 906, 1156
CviQI GTAC 1 cut(s) 1265
DdeI CTNAG 6 cut(s) 267, 303, 770, 1126, 1145, 1187
DpnI GATC 7 cut(s) 467, 665, 696, 799, 1062, 1253, 1271
DpnII GATC 7 cut(s) 465, 663, 694, 797, 1060, 1251, 1269
EaeI YGGCCR 1 cut(s) 36
Eam1104I CTCTTC 4 cut(s) 159, 446, 818, 1061
EarI CTCTTC 4 cut(s) 159, 446, 818, 1061
Eco130I CCWWGG 1 cut(s) 630
Eco147I AGGCCT 1 cut(s) 501
Eco47I GGWCC 3 cut(s) 82, 578, 634
Eco57I CTGAAG 1 cut(s) 740
EcoO109I RGGNCCY 2 cut(s) 82, 578
EcoRII CCWGG 2 cut(s) 119, 580
EcoT14I CCWWGG 1 cut(s) 630
EcoT22I ATGCAT 1 cut(s) 717
ErhI CCWWGG 1 cut(s) 630
Esp3I CGTCTC 1 cut(s) 810
FaeI CATG 5 cut(s) 104, 634, 659, 909, 1159
FalI AAGNNNNNCTT 4 cut(s) 214, 246, 1109, 1141
FaqI GGGAC 3 cut(s) 170, 901, 1091
FatI CATG 5 cut(s) 100, 630, 655, 905, 1155
FbaI TGATCA 1 cut(s) 1060
Fnu4HI GCNGC 8 cut(s) 181, 184, 187, 311, 586, 879, 903, 1131
FokI GGATG 4 cut(s) 151, 257, 877, 1088
Fsp4HI GCNGC 8 cut(s) 181, 184, 187, 311, 586, 879, 903, 1131
FspBI CTAG 2 cut(s) 152, 779
GluI GCNGC 8 cut(s) 181, 184, 187, 311, 586, 879, 903, 1131
HaeIII GGCC 4 cut(s) 38, 501, 679, 971
HapII CCGG 4 cut(s) 591, 748, 809, 884
HgaI GACGC 2 cut(s) 823, 898
Hin1I GRCGYC 1 cut(s) 890
Hin1II CATG 5 cut(s) 104, 634, 659, 909, 1159
HindIII AAGCTT 1 cut(s) 842
HinfI GANTC 8 cut(s) 158, 271, 413, 522, 548, 757, 766, 1137
HpaII CCGG 4 cut(s) 591, 748, 809, 884
HphI GGTGA 1 cut(s) 823
Hpy166II GTNNAC 1 cut(s) 1243
Hpy188III TCNNGA 6 cut(s) 251, 332, 422, 479, 559, 754
Hpy8I GTNNAC 1 cut(s) 1243
Hpy99I CGWCG 3 cut(s) 320, 671, 805
HpyAV CCTTC 5 cut(s) 37, 170, 409, 715, 985
HpyCH4III ACNGT 3 cut(s) 534, 604, 1170
HpyCH4IV ACGT 3 cut(s) 318, 669, 1101
HpyCH4V TGCA 7 cut(s) 14, 142, 313, 715, 849, 1133, 1285
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 44, 186, 591, 748
HpyF3I CTNAG 6 cut(s) 267, 303, 770, 1126, 1145, 1187
HpySE526I ACGT 3 cut(s) 318, 669, 1101
Hsp92I GRCGYC 1 cut(s) 890
Hsp92II CATG 5 cut(s) 104, 634, 659, 909, 1159
Ksp22I TGATCA 1 cut(s) 1060
Kzo9I GATC 7 cut(s) 465, 663, 694, 797, 1060, 1251, 1269
LmnI GCTCC 3 cut(s) 508, 593, 886
Lsp1109I GCAGC 5 cut(s) 192, 198, 297, 865, 1117
LweI GCATC 5 cut(s) 129, 235, 702, 724, 1066
MaeI CTAG 2 cut(s) 152, 779
MaeII ACGT 3 cut(s) 318, 669, 1101
MaeIII GTNAC 3 cut(s) 319, 672, 811
MalI GATC 7 cut(s) 467, 665, 696, 799, 1062, 1253, 1271
MboI GATC 7 cut(s) 465, 663, 694, 797, 1060, 1251, 1269
MfeI CAATTG 1 cut(s) 818
MflI RGATCY 1 cut(s) 1251
MhlI GDGCHC 1 cut(s) 776
MluCI AATT 4 cut(s) 15, 390, 818, 944
MlyI GAGTC 3 cut(s) 152, 516, 760
MmeI TCCRAC 1 cut(s) 57
Mph1103I ATGCAT 1 cut(s) 717
MroXI GAANNNNTTC 1 cut(s) 1037
MseI TTAA 2 cut(s) 372, 933
MslI CAYNNNNRTG 2 cut(s) 105, 605
MspA1I CMGCKG 1 cut(s) 183
MspI CCGG 4 cut(s) 591, 748, 809, 884
MspR9I CCNGG 3 cut(s) 121, 582, 885
MunI CAATTG 1 cut(s) 818
Mva1269I GAATGC 1 cut(s) 849
MvaI CCWGG 2 cut(s) 121, 582
MvnI CGCG 1 cut(s) 333
MwoI GCNNNNNNNGC 5 cut(s) 11, 44, 186, 591, 748
NciI CCSGG 1 cut(s) 885
NcoI CCATGG 1 cut(s) 630
NdeII GATC 7 cut(s) 465, 663, 694, 797, 1060, 1251, 1269
NheI GCTAGC 1 cut(s) 778
NlaIII CATG 5 cut(s) 104, 634, 659, 909, 1159
NlaIV GGNNCC 3 cut(s) 84, 510, 589
NmeAIII GCCGAG 1 cut(s) 64
NmuCI GTSAC 2 cut(s) 672, 811
NruI TCGCGA 1 cut(s) 333
NsiI ATGCAT 1 cut(s) 717
NspI RCATGY 1 cut(s) 1159
OliI CACNNNNGTG 1 cut(s) 605
PceI AGGCCT 1 cut(s) 501
PcsI WCGNNNNNNNCGW 1 cut(s) 969
PctI GAATGC 1 cut(s) 849
PdmI GAANNNNTTC 1 cut(s) 1037
PfeI GAWTC 5 cut(s) 271, 413, 548, 757, 1137
PflFI GACNNNGTC 2 cut(s) 892, 1103
PflMI CCANNNNNTGG 1 cut(s) 226
PkrI GCNGC 8 cut(s) 182, 185, 188, 312, 587, 880, 904, 1132
PleI GAGTC 3 cut(s) 152, 516, 760
PpsI GAGTC 3 cut(s) 152, 516, 760
PpuMI RGGWCCY 2 cut(s) 82, 578
Psp5II RGGWCCY 2 cut(s) 82, 578
Psp6I CCWGG 2 cut(s) 119, 580
PspGI CCWGG 2 cut(s) 119, 580
PspN4I GGNNCC 3 cut(s) 84, 510, 589
PspPI GGNCC 3 cut(s) 82, 578, 634
PspPPI RGGWCCY 2 cut(s) 82, 578
PsrI GAACNNNNNNTAC 2 cut(s) 1224, 1256
PstNI CAGNNNCTG 1 cut(s) 310
PsuI RGATCY 1 cut(s) 1251
PsyI GACNNNGTC 2 cut(s) 892, 1103
RruI TCGCGA 1 cut(s) 333
RsaI GTAC 1 cut(s) 1266
RsaNI GTAC 1 cut(s) 1265
RseI CAYNNNNRTG 2 cut(s) 105, 605
SaqAI TTAA 2 cut(s) 372, 933
SatI GCNGC 8 cut(s) 181, 184, 187, 311, 586, 879, 903, 1131
Sau3AI GATC 7 cut(s) 465, 663, 694, 797, 1060, 1251, 1269
Sau96I GGNCC 3 cut(s) 82, 578, 634
SchI GAGTC 3 cut(s) 152, 516, 760
ScrFI CCNGG 3 cut(s) 121, 582, 885
SduI GDGCHC 1 cut(s) 776
SfaNI GCATC 5 cut(s) 129, 235, 702, 724, 1066
SinI GGWCC 3 cut(s) 82, 578, 634
SmiMI CAYNNNNRTG 2 cut(s) 105, 605
SmlI CTYRAG 5 cut(s) 251, 422, 479, 503, 557
SmoI CTYRAG 5 cut(s) 251, 422, 479, 503, 557
Sse9I AATT 4 cut(s) 15, 390, 818, 944
SseBI AGGCCT 1 cut(s) 501
SsiI CCGC 3 cut(s) 183, 585, 902
SspMI CTAG 2 cut(s) 152, 779
StuI AGGCCT 1 cut(s) 501
StyD4I CCNGG 3 cut(s) 119, 580, 883
StyI CCWWGG 1 cut(s) 630
TaaI ACNGT 3 cut(s) 534, 604, 1170
TaiI ACGT 3 cut(s) 321, 672, 1104
TaqI TCGA 2 cut(s) 666, 918
TaqII GACCGA 1 cut(s) 78
TasI AATT 4 cut(s) 15, 390, 818, 944
TatI WGTACW 1 cut(s) 1264
TauI GCSGC 3 cut(s) 186, 588, 905
TfiI GAWTC 5 cut(s) 271, 413, 548, 757, 1137
Tru1I TTAA 2 cut(s) 372, 933
Tru9I TTAA 2 cut(s) 372, 933
TseFI GTSAC 2 cut(s) 672, 811
TseI GCWGC 5 cut(s) 180, 186, 310, 878, 1130
Tsp45I GTSAC 2 cut(s) 672, 811
TspDTI ATGAA 4 cut(s) 189, 249, 250, 504
Tth111I GACNNNGTC 2 cut(s) 892, 1103
Van91I CCANNNNNTGG 1 cut(s) 226
VpaK11BI GGWCC 3 cut(s) 82, 578, 634
XapI RAATTY 1 cut(s) 944
XceI RCATGY 1 cut(s) 1159
XmnI GAANNNNTTC 1 cut(s) 1037
XspI CTAG 2 cut(s) 152, 779
Zsp2I ATGCAT 1 cut(s) 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.