Rroxscaffold_1G00051800

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000865
Physical Location & Seq
Reverse (-)
72572858 .. 72575872
3015 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00051800.1

Sequence Viewer

Length: 2670 bp
ATGGCTGAACTAGCAATTCCTTTATCATTGTTGGTGGCCGAGAAGGCGATGAGCTTGTGCGACCAAATATACAAGTCCAGGGACCGAGGTAATAATATCCATGATGATGTTGAAATAGCCTGGAAATGTCTGAAGATGATGCAAGCCTATCTAGGGGACTCGGAGATTGATGAAGGCAGCCAAATGCTTCAAAATCGTGTCGCAGAGGTCCAAGATCTGGCTTATGAAATAGAGGATGCTCTTGACAAGTTCATGTCTCAGATTCCACATCATTTCCACAGCAACAAATTCTCTCAGAAGCTGCACGACGTTGCGCACTATCGCACAGAAAAGAACGCAAGAGTTGAGTTCTCCACCAAGATTAAAGAACTTGAAAAGAAAATTGTTTCCTTCTCAACTTTGGATTCACTTCTTGAGCCACCAAGTAGTTCCAGCACAAGGCAGAGGGATGGTTTGATCCACCAAGTTCTTGAGGAAGATGAAATAGTAGGCCTTGAAGAGCCTAAAGAAAGACTCTCCAAACAGTTGATGGAAGAAGATTCAAGGCTCACGACTATTTTGGTTGTAGGTCCTGGAGGCTCCGGCAAAACCACAGTTGTGAAGAATGTTTTTGATAGCAAAATGGTCCAACGGTTTTTTGATTGCCATGCTTGGATCGACGTGTCACGGCCTCCTAAAGCAGAAGATCAGAAAGCGGAACTGAGGCTCCTTAAGGAGAAGAGATTTTTGGTTGTTTTGGATAATGTTTGGAGAGCACAAGATCTGGAACTCATTACAAACGCTTTGCCAAGAGGATTGCCTGGGAGTAAAGTAATCATAACTACTCGCAACTCTGATGTAGCTTCTTCTCGTGTTGAGTCCTCCGAGTATATCCATGATTTGAGTGGTGGGTTATCATGGAACAAAGGCTGGTCTCTACTTTGCAAGAAGGCTTTTAGAAAAACTGCAGGATATTGTCCACCTGAACTTGAAGAGTGGGCTAAAAAAATCCTGAAGAAGTGTGGAGGTCTGCCCCTTGCAATTTCAGCTGTTGCTAGTTTGCTGGCAAAGAAGCCACAAACTCCAGTTGAATGGAAGAAGTTACATGAAAGCCTTGGGTCTGAAATTAGACCCGACTTTAGTCTTTCGATAATTAGCCGTGTCTTGCAGCCAAGTTACATGCATCTTCCGAGCCATCTGAAGACTTGTTTCTTGTACTTCGGCATGTTTCCAGAAGACTATTCAATCAGCCGTGACAGGCTGATTCGGTTGTGGTTAGCCGAAGGATTTGTGGTGCCAGGAAGAGGACAAACAACGGAGGAGGCTGCAGAGAGTTATCTGAATGAACTCATTGGAAGAAATTTGGTACATGCCACCACAAGGGAGATTAATGGTCGAGTAAGGAGCTGTGGTGTTTCTAACCTTGTTCGAGAGTTCATCATTTCAAAGGCAGGGAACTTCATCACGGTTTTGGATCCAAACTGCCCTAGTACTACTCATTCAAGTGAGAAAATTCGACGTCTTTCAATTCAAAATGTTAATTTATTAAGCGGTAGAGACTTCAGTCATATTCGTACCTTACTAGTGTTTGGGAAGGAGTGTTCCCCTTCTGAGCTTGAAAATGTGCTCAAGACCTCTAAATTTTTAAGGGTGTTAGATTTTGAAGGTGTACCTTTCAATAACTTTCCTGACAGTGTTATTAGTCTCCCCCTCTTGAGGTACTTATGTCTGAGCCAAACCGATCTTAAATCAGTTCCAAAGTCCATTGGCAATCTTGCATTCTTGCAAACCTTAGATCTTAAGCACACACAGGTGACCAACTTACCAAAACAGATTTATGAGCTTCAGGATATGCGTCATCTTCTAGTTTATAGCTGTGATGTGACTTCTGGAGTGGCACAAGGAGTTGAGATTTGTGCAGGCAATATTGGAGCTTTATCTTCCTTACAGAAGTTGTTTCTAATTAAGGTGAAAACACAAAGAAAAGTAGTCCTTAAAGCCTTGGGAGAGTTAATTGCTCTCCGGAAGTTGGGGCTGCAGGTAGACCTTGAAAGAGAACATGGAAGAGAATTGTGTTGTTCCATTCAAAAAATGCCAGAGCTTTCAACACTTGATGTACAATCAACAAGCGAAGATGAGTATCTTGATTTGGATCAACTGAATTCCCCTCCCGGGCCTCCCCGCTATCTCCAACGTCTATCTTTGACAGGGCGCCTAGAAAGTCTACCACAGTGGATTCTCCAGCTTCACAGTCTAGCTAGGATTGCTTTGAAGTGGTCTAAACTGAATGCTGATGTCAAGTCACTTGAGGCGCTCCAAGATTTGCCTAATCTGTTGGAGCTACAGTTGGTGGACTATTTCACTGGGGATTCATTGAAATTCAAAGCCAAAAAGTTCAAGAAACTAAAGGTATTACGTATTGAGCAACTTGATCAGCTAGATATGGTGGTAGTCGAGGATGGAGCAATGCCCGAGCTTAAGAAGTTAACATTGTCTAGATGTCAGAATCTGAAGTTACTTCCATATGGCATTGATAGGCTTATCTTGCTTGAAGAATTGATTCTGTACGACATGCCCGAAGAATTCATTGCTAGGCTTCAGAAGGATAGCGAAGACCGTTACTTGGTTGAGAATCTTCGAGTCATCCAATCTTTCTATTCTGGGAGCACTGGATACCAAAATCTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

889

Amino Acids

100.75

Weight (kDa)

7.03

Isoelectric Point (pI)

47.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 28 - 100 1.2e-08 Rx N-terminal domain
NB-ARC PF00931 166 - 224 1.7e-11 NB-ARC domain
nSTAND_NTPase5 PF25199 171 - 284 2.1e-07 Novel STAND NTPase 5
NB-ARC PF00931 234 - 316 4.3e-15 NB-ARC domain
WHD_DRP PF23559 402 - 473 1.2e-18 Disease resistance protein Winged helix domain
LRR_14 PF23598 516 - 846 3.4e-44 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1501
Acc16I TGCGCA 1 cut(s) 315
Acc36I ACCTGC 1 cut(s) 2008
AccB1I GGYRCC 2 cut(s) 1273, 2190
AccB7I CCANNNNNTGG 1 cut(s) 217
AccI GTMKAC 2 cut(s) 2022, 2203
AccIII TCCGGA 1 cut(s) 2001
AciI CCGC 3 cut(s) 695, 1530, 2161
AclWI GGATC 5 cut(s) 451, 662, 1448, 1461, 2139
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 7 cut(s) 287, 1339, 1491, 1619, 2140, 2357, 2561
AcuI CTGAAG 7 cut(s) 152, 1013, 1199, 1525, 1808, 2510, 2561
AcyI GRCGYC 2 cut(s) 1498, 2191
AfaI GTAC 8 cut(s) 1196, 1347, 1471, 1555, 1650, 1700, 2097, 2546
AfiI CCNNNNNNNGG 9 cut(s) 153, 217, 438, 1283, 1359, 1695, 2008, 2151, 2602
AflII CTTAAG 3 cut(s) 710, 1778, 2456
AflIII ACRYGT 1 cut(s) 660
AhlI ACTAGT 1 cut(s) 1561
AjiI CACGTC 1 cut(s) 661
AjnI CCWGG 5 cut(s) 77, 119, 571, 799, 1276
AjuI GAANNNNNNNTTGG 2 cut(s) 710, 742
AleI CACNNNNGTG 2 cut(s) 596, 1790
AloI GAACNNNNNNTCC 4 cut(s) 690, 722, 1564, 1596
Alw21I GWGCWC 3 cut(s) 757, 1608, 2648
Alw26I GTCTC 4 cut(s) 261, 918, 1530, 1688
AlwI GGATC 5 cut(s) 451, 662, 1448, 1461, 2139
AlwNI CAGNNNCTG 2 cut(s) 301, 2488
Ama87I CYCGRG 2 cut(s) 2150, 2450
Aor13HI TCCGGA 1 cut(s) 2001
AoxI GGCC 4 cut(s) 36, 490, 668, 2153
ApeKI GCWGC 5 cut(s) 177, 301, 1147, 1304, 2014
ApoI RAATTY 7 cut(s) 287, 1339, 1491, 1619, 2140, 2357, 2561
AseI ATTAAT 1 cut(s) 1368
Asp700I GAANNNNTTC 1 cut(s) 2538
AspLEI GCGC 3 cut(s) 316, 2193, 2293
AspS9I GGNCC 5 cut(s) 82, 208, 569, 625, 2153
AsuC2I CCSGG 2 cut(s) 2151, 2152
AsuHPI GGTGA 2 cut(s) 1804, 1960
AvaI CYCGRG 2 cut(s) 2150, 2450
AvaII GGWCC 4 cut(s) 82, 208, 569, 625
BaeI ACNNNNGTAYC 2 cut(s) 1690, 1723
BamHI GGATCC 1 cut(s) 1453
BanI GGYRCC 2 cut(s) 1273, 2190
BauI CACGAG 1 cut(s) 849
BbsI GAAGAC 3 cut(s) 1187, 1221, 2598
Bbv12I GWGCWC 3 cut(s) 757, 1608, 2648
BbvI GCAGC 5 cut(s) 189, 288, 1159, 1291, 2001
BccI CCATC 4 cut(s) 443, 523, 1182, 2432
BceAI ACGGC 3 cut(s) 683, 1122, 1215
BciT130I CCWGG 5 cut(s) 79, 121, 573, 801, 1278
BciVI GTATCC 1 cut(s) 2645
BclI TGATCA 1 cut(s) 2410
BcnI CCSGG 2 cut(s) 2151, 2152
BcoDI GTCTC 4 cut(s) 261, 918, 1530, 1688
BcuI ACTAGT 1 cut(s) 1561
BfmI CTRYAG 4 cut(s) 945, 1305, 2015, 2321
BfoI RGCGCY 2 cut(s) 2194, 2294
BfrI CTTAAG 3 cut(s) 710, 1778, 2456
BfuAI ACCTGC 1 cut(s) 2008
BfuI GTATCC 1 cut(s) 2645
BglI GCCNNNNNGGC 1 cut(s) 44
BglII AGATCT 3 cut(s) 214, 760, 1774
BisI GCNGC 5 cut(s) 178, 302, 1148, 1305, 2015
BlsI GCNGC 5 cut(s) 179, 303, 1149, 1306, 2016
BmcAI AGTACT 1 cut(s) 1471
Bme1390I CCNGG 7 cut(s) 79, 121, 573, 801, 1278, 2151, 2152
Bme18I GGWCC 4 cut(s) 82, 208, 569, 625
BmeT110I CYCGRG 2 cut(s) 2150, 2450
BmgBI CACGTC 1 cut(s) 661
BmgT120I GGNCC 5 cut(s) 82, 208, 569, 625, 2153
BmiI GGNNCC 6 cut(s) 83, 580, 707, 1275, 1455, 2192
BmrFI CCNGG 7 cut(s) 79, 121, 573, 801, 1278, 2151, 2152
BmrI ACTGGG 1 cut(s) 2352
BmsI GCATC 3 cut(s) 129, 226, 1171
BmuI ACTGGG 1 cut(s) 2352
BoxI GACNNNNGTC 2 cut(s) 1119, 1542
BpiI GAAGAC 3 cut(s) 1187, 1221, 2598
BpmI CTGGAG 4 cut(s) 594, 1047, 1890, 2204
BpuEI CTTGAG 5 cut(s) 434, 491, 1592, 1714, 2306
BpuMI CCSGG 2 cut(s) 2151, 2152
BsaAI YACGTR 1 cut(s) 2396
BsaBI GATNNNNATC 2 cut(s) 2118, 2130
BsaHI GRCGYC 2 cut(s) 1498, 2191
BsaI GGTCTC 1 cut(s) 918
BsaJI CCNNGG 6 cut(s) 78, 85, 800, 1093, 1980, 2150
BsaWI WCCGGW 1 cut(s) 2001
BsaXI ACNNNNNCTCC 6 cut(s) 690, 720, 1375, 1405, 2309, 2339
Bsc4I CCNNNNNNNGG 9 cut(s) 153, 217, 438, 1283, 1359, 1695, 2008, 2151, 2602
Bse1I ACTGG 3 cut(s) 1064, 2347, 2653
Bse3DI GCAATG 2 cut(s) 2451, 2565
Bse8I GATNNNNATC 2 cut(s) 2118, 2130
BseAI TCCGGA 1 cut(s) 2001
BseBI CCWGG 5 cut(s) 79, 121, 573, 801, 1278
BseDI CCNNGG 6 cut(s) 78, 85, 800, 1093, 1980, 2150
BseGI GGATG 4 cut(s) 241, 454, 2443, 2622
BseJI GATNNNNATC 2 cut(s) 2118, 2130
BseLI CCNNNNNNNGG 9 cut(s) 153, 217, 438, 1283, 1359, 1695, 2008, 2151, 2602
BseMI GCAATG 2 cut(s) 2451, 2565
BseMII CTCAG 5 cut(s) 272, 308, 692, 1581, 1700
BseNI ACTGG 3 cut(s) 1064, 2347, 2653
BseRI GAGGAG 1 cut(s) 1313
BseXI GCAGC 5 cut(s) 189, 288, 1159, 1291, 2001
BsgI GTGCAG 2 cut(s) 287, 1917
BshFI GGCC 4 cut(s) 38, 492, 670, 2155
BshNI GGYRCC 2 cut(s) 1273, 2190
BsiHKAI GWGCWC 3 cut(s) 757, 1608, 2648
BsiHKCI CYCGRG 2 cut(s) 2150, 2450
BsiSI CCGG 3 cut(s) 582, 2002, 2151
BslFI GGGAC 2 cut(s) 95, 170
BslI CCNNNNNNNGG 9 cut(s) 153, 217, 438, 1283, 1359, 1695, 2008, 2151, 2602
BsmAI GTCTC 4 cut(s) 261, 918, 1530, 1688
BsmFI GGGAC 2 cut(s) 95, 170
BsmI GAATGC 2 cut(s) 1757, 2272
BsnI GGCC 4 cut(s) 38, 492, 670, 2155
Bso31I GGTCTC 1 cut(s) 918
BsoBI CYCGRG 2 cut(s) 2150, 2450
Bsp1286I GDGCHC 3 cut(s) 757, 1608, 2648
Bsp13I TCCGGA 1 cut(s) 2001
Bsp1407I TGTACA 1 cut(s) 2095
BspACI CCGC 3 cut(s) 695, 1530, 2161
BspANI GGCC 4 cut(s) 38, 492, 670, 2155
BspCNI CTCAG 5 cut(s) 271, 307, 693, 1582, 1701
BspEI TCCGGA 1 cut(s) 2001
BspLI GGNNCC 6 cut(s) 83, 580, 707, 1275, 1455, 2192
BspMAI CTGCAG 3 cut(s) 949, 1309, 2019
BspMI ACCTGC 1 cut(s) 2008
BspPI GGATC 5 cut(s) 451, 662, 1448, 1461, 2139
BspQI GCTCTTC 1 cut(s) 492
BspT107I GGYRCC 2 cut(s) 1273, 2190
BspTI CTTAAG 3 cut(s) 710, 1778, 2456
BspTNI GGTCTC 1 cut(s) 918
BsrDI GCAATG 2 cut(s) 2451, 2565
BsrGI TGTACA 1 cut(s) 2095
BsrI ACTGG 3 cut(s) 1064, 2347, 2653
BssECI CCNNGG 6 cut(s) 78, 85, 800, 1093, 1980, 2150
BssNI GRCGYC 2 cut(s) 1498, 2191
BssSI CACGAG 1 cut(s) 849
BssT1I CCWWGG 2 cut(s) 1093, 1980
Bst2BI CACGAG 1 cut(s) 849
Bst2UI CCWGG 5 cut(s) 79, 121, 573, 801, 1278
Bst4CI ACNGT 9 cut(s) 525, 595, 633, 1447, 1673, 2211, 2231, 2325, 2597
Bst6I CTCTTC 5 cut(s) 492, 713, 966, 1276, 2038
BstACI GRCGYC 2 cut(s) 1498, 2191
BstAFI CTTAAG 3 cut(s) 710, 1778, 2456
BstAUI TGTACA 1 cut(s) 2095
BstBAI YACGTR 1 cut(s) 2396
BstC8I GCNNGC 3 cut(s) 144, 1044, 1900
BstDEI CTNAG 6 cut(s) 258, 294, 701, 1590, 1709, 1771
BstEII GGTNACC 1 cut(s) 1792
BstF5I GGATG 4 cut(s) 241, 454, 2443, 2622
BstH2I RGCGCY 2 cut(s) 2194, 2294
BstHHI GCGC 3 cut(s) 316, 2193, 2293
BstMAI GTCTC 4 cut(s) 261, 918, 1530, 1688
BstMWI GCNNNNNNNGC 5 cut(s) 11, 44, 1025, 2243, 2524
BstNI CCWGG 5 cut(s) 79, 121, 573, 801, 1278
BstNSI RCATGY 4 cut(s) 1162, 1207, 1352, 2554
BstPAI GACNNNNGTC 2 cut(s) 1119, 1542
BstPI GGTNACC 1 cut(s) 1792
BstSCI CCNGG 7 cut(s) 77, 119, 571, 799, 1276, 2149, 2150
BstSFI CTRYAG 4 cut(s) 945, 1305, 2015, 2321
BstSNI TACGTA 1 cut(s) 2396
BstV1I GCAGC 5 cut(s) 189, 288, 1159, 1291, 2001
BstV2I GAAGAC 3 cut(s) 1187, 1221, 2598
BstX2I RGATCY 4 cut(s) 214, 760, 1453, 1774
BstXI CCANNNNNNTGG 1 cut(s) 1071
BstYI RGATCY 4 cut(s) 214, 760, 1453, 1774
BsuI GTATCC 1 cut(s) 2645
BsuRI GGCC 4 cut(s) 38, 492, 670, 2155
BtgZI GCGATG 1 cut(s) 62
BtrI CACGTC 1 cut(s) 661
BtsCI GGATG 4 cut(s) 241, 454, 2443, 2622
BtsIMutI CAGTG 4 cut(s) 1678, 2216, 2340, 2646
BveI ACCTGC 1 cut(s) 2008
Cac8I GCNNGC 3 cut(s) 144, 1044, 1900
CaiI CAGNNNCTG 2 cut(s) 301, 2488
CfoI GCGC 3 cut(s) 316, 2193, 2293
Cfr13I GGNCC 5 cut(s) 82, 208, 569, 625, 2153
Cfr9I CCCGGG 1 cut(s) 2150
CseI GACGC 1 cut(s) 1823
Csp6I GTAC 8 cut(s) 1195, 1346, 1470, 1554, 1649, 1699, 2096, 2545
CviQI GTAC 8 cut(s) 1195, 1346, 1470, 1554, 1649, 1699, 2096, 2545
DdeI CTNAG 6 cut(s) 258, 294, 701, 1590, 1709, 1771
DinI GGCGCC 1 cut(s) 2192
EaeI YGGCCR 1 cut(s) 36
Eam1104I CTCTTC 5 cut(s) 492, 713, 966, 1276, 2038
EarI CTCTTC 5 cut(s) 492, 713, 966, 1276, 2038
Eco105I TACGTA 1 cut(s) 2396
Eco130I CCWWGG 2 cut(s) 1093, 1980
Eco147I AGGCCT 1 cut(s) 492
Eco31I GGTCTC 1 cut(s) 918
Eco47I GGWCC 4 cut(s) 82, 208, 569, 625
Eco57I CTGAAG 7 cut(s) 152, 1013, 1199, 1525, 1808, 2510, 2561
Eco88I CYCGRG 2 cut(s) 2150, 2450
Eco91I GGTNACC 1 cut(s) 1792
EcoO109I RGGNCCY 1 cut(s) 569
EcoO65I GGTNACC 1 cut(s) 1792
EcoRI GAATTC 2 cut(s) 2140, 2561
EcoRII CCWGG 5 cut(s) 77, 119, 571, 799, 1276
EcoT14I CCWWGG 2 cut(s) 1093, 1980
EcoT22I ATGCAT 1 cut(s) 1164
EgeI GGCGCC 1 cut(s) 2192
EheI GGCGCC 1 cut(s) 2192
ErhI CCWWGG 2 cut(s) 1093, 1980
FalI AAGNNNNNCTT 2 cut(s) 1956, 1988
FaqI GGGAC 2 cut(s) 95, 170
FauI CCCGC 1 cut(s) 2168
FauNDI CATATG 1 cut(s) 2503
FbaI TGATCA 1 cut(s) 2410
FblI GTMKAC 2 cut(s) 2022, 2203
Fnu4HI GCNGC 5 cut(s) 178, 302, 1148, 1305, 2015
FokI GGATG 4 cut(s) 248, 461, 2450, 2609
Fsp4HI GCNGC 5 cut(s) 178, 302, 1148, 1305, 2015
FspI TGCGCA 1 cut(s) 315
GlaI GCGC 3 cut(s) 315, 2192, 2292
GluI GCNGC 5 cut(s) 178, 302, 1148, 1305, 2015
GsuI CTGGAG 4 cut(s) 594, 1047, 1890, 2204
HaeII RGCGCY 2 cut(s) 2194, 2294
HaeIII GGCC 4 cut(s) 38, 492, 670, 2155
HapII CCGG 3 cut(s) 582, 2002, 2151
HgaI GACGC 1 cut(s) 1823
HhaI GCGC 3 cut(s) 316, 2193, 2293
Hin1I GRCGYC 2 cut(s) 1498, 2191
Hin6I GCGC 3 cut(s) 314, 2191, 2291
HinP1I GCGC 3 cut(s) 314, 2191, 2291
HincII GTYRAC 1 cut(s) 2466
HindII GTYRAC 1 cut(s) 2466
HpaI GTTAAC 1 cut(s) 2466
HpaII CCGG 3 cut(s) 582, 2002, 2151
HphI GGTGA 2 cut(s) 1804, 1960
Hpy166II GTNNAC 6 cut(s) 959, 1649, 2023, 2204, 2332, 2466
Hpy8I GTNNAC 6 cut(s) 959, 1649, 2023, 2204, 2332, 2466
Hpy99I CGWCG 3 cut(s) 311, 662, 1500
HpyAV CCTTC 9 cut(s) 37, 167, 400, 922, 1256, 1567, 1596, 1637, 2575
HpyCH4III ACNGT 9 cut(s) 525, 595, 633, 1447, 1673, 2211, 2231, 2325, 2597
HpyCH4IV ACGT 5 cut(s) 309, 660, 1498, 2173, 2395
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 44, 1025, 2243, 2524
HpyF3I CTNAG 6 cut(s) 258, 294, 701, 1590, 1709, 1771
HpySE526I ACGT 5 cut(s) 309, 660, 1498, 2173, 2395
Hsp92I GRCGYC 2 cut(s) 1498, 2191
HspAI GCGC 3 cut(s) 314, 2191, 2291
KasI GGCGCC 1 cut(s) 2190
Kpn2I TCCGGA 1 cut(s) 2001
Ksp22I TGATCA 1 cut(s) 2410
KspAI GTTAAC 1 cut(s) 2466
LguI GCTCTTC 1 cut(s) 492
LmnI GCTCC 8 cut(s) 584, 711, 1383, 1910, 2298, 2317, 2441, 2643
Lsp1109I GCAGC 5 cut(s) 189, 288, 1159, 1291, 2001
LweI GCATC 3 cut(s) 129, 226, 1171
MaeII ACGT 5 cut(s) 309, 660, 1498, 2173, 2395
MaeIII GTNAC 9 cut(s) 663, 1080, 1154, 1232, 1792, 1861, 2280, 2493, 2597
MflI RGATCY 4 cut(s) 214, 760, 1453, 1774
MhlI GDGCHC 3 cut(s) 757, 1608, 2648
Mly113I GGCGCC 1 cut(s) 2191
MlyI GAGTC 4 cut(s) 152, 507, 866, 2628
MmeI TCCRAC 3 cut(s) 652, 2194, 2295
Mph1103I ATGCAT 1 cut(s) 1164
MroI TCCGGA 1 cut(s) 2001
MroXI GAANNNNTTC 1 cut(s) 2538
MslI CAYNNNNRTG 4 cut(s) 105, 596, 1482, 1790
MspA1I CMGCKG 1 cut(s) 1028
MspCI CTTAAG 3 cut(s) 710, 1778, 2456
MspI CCGG 3 cut(s) 582, 2002, 2151
MspR9I CCNGG 7 cut(s) 79, 121, 573, 801, 1278, 2151, 2152
Mva1269I GAATGC 2 cut(s) 1757, 2272
MvaI CCWGG 5 cut(s) 79, 121, 573, 801, 1278
MwoI GCNNNNNNNGC 5 cut(s) 11, 44, 1025, 2243, 2524
NarI GGCGCC 1 cut(s) 2191
NciI CCSGG 2 cut(s) 2151, 2152
NdeI CATATG 1 cut(s) 2503
NlaIV GGNNCC 6 cut(s) 83, 580, 707, 1275, 1455, 2192
NmeAIII GCCGAG 1 cut(s) 64
NmuCI GTSAC 5 cut(s) 663, 1232, 1792, 1861, 2280
NsbI TGCGCA 1 cut(s) 315
NsiI ATGCAT 1 cut(s) 1164
NspI RCATGY 4 cut(s) 1162, 1207, 1352, 2554
OliI CACNNNNGTG 2 cut(s) 596, 1790
PceI AGGCCT 1 cut(s) 492
PciSI GCTCTTC 1 cut(s) 492
PcsI WCGNNNNNNNCGW 1 cut(s) 2553
PctI GAATGC 2 cut(s) 1757, 2272
PdmI GAANNNNTTC 1 cut(s) 2538
PfeI GAWTC 9 cut(s) 262, 404, 539, 1243, 2215, 2348, 2485, 2539, 2611
PflMI CCANNNNNTGG 1 cut(s) 217
PfoI TCCNGGA 1 cut(s) 571
PkrI GCNGC 5 cut(s) 179, 303, 1149, 1306, 2016
PleI GAGTC 4 cut(s) 152, 507, 865, 2627
PluTI GGCGCC 1 cut(s) 2194
PpsI GAGTC 4 cut(s) 152, 507, 865, 2627
Ppu21I YACGTR 1 cut(s) 2396
PpuMI RGGWCCY 1 cut(s) 569
PshAI GACNNNNGTC 2 cut(s) 1119, 1542
PshBI ATTAAT 1 cut(s) 1368
Psp5II RGGWCCY 1 cut(s) 569
Psp6I CCWGG 5 cut(s) 77, 119, 571, 799, 1276
PspEI GGTNACC 1 cut(s) 1792
PspGI CCWGG 5 cut(s) 77, 119, 571, 799, 1276
PspN4I GGNNCC 6 cut(s) 83, 580, 707, 1275, 1455, 2192
PspPI GGNCC 5 cut(s) 82, 208, 569, 625, 2153
PspPPI RGGWCCY 1 cut(s) 569
PstI CTGCAG 3 cut(s) 949, 1309, 2019
PstNI CAGNNNCTG 2 cut(s) 301, 2488
PsuI RGATCY 4 cut(s) 214, 760, 1453, 1774
PvuII CAGCTG 1 cut(s) 1028
RsaI GTAC 8 cut(s) 1196, 1347, 1471, 1555, 1650, 1700, 2097, 2546
RsaNI GTAC 8 cut(s) 1195, 1346, 1470, 1554, 1649, 1699, 2096, 2545
RseI CAYNNNNRTG 4 cut(s) 105, 596, 1482, 1790
SapI GCTCTTC 1 cut(s) 492
SatI GCNGC 5 cut(s) 178, 302, 1148, 1305, 2015
Sau96I GGNCC 5 cut(s) 82, 208, 569, 625, 2153
ScaI AGTACT 1 cut(s) 1471
SchI GAGTC 4 cut(s) 152, 507, 866, 2628
ScrFI CCNGG 7 cut(s) 79, 121, 573, 801, 1278, 2151, 2152
SduI GDGCHC 3 cut(s) 757, 1608, 2648
SfaNI GCATC 3 cut(s) 129, 226, 1171
SfcI CTRYAG 4 cut(s) 945, 1305, 2015, 2321
SfoI GGCGCC 1 cut(s) 2192
SinI GGWCC 4 cut(s) 82, 208, 569, 625
SmaI CCCGGG 1 cut(s) 2152
SmiMI CAYNNNNRTG 4 cut(s) 105, 596, 1482, 1790
SmlI CTYRAG 8 cut(s) 413, 470, 710, 1607, 1693, 1778, 2285, 2456
SmoI CTYRAG 8 cut(s) 413, 470, 710, 1607, 1693, 1778, 2285, 2456
SnaBI TACGTA 1 cut(s) 2396
SpeI ACTAGT 1 cut(s) 1561
SseBI AGGCCT 1 cut(s) 492
SsiI CCGC 3 cut(s) 695, 1530, 2161
SspDI GGCGCC 1 cut(s) 2190
SspI AATATT 1 cut(s) 1906
StuI AGGCCT 1 cut(s) 492
StyD4I CCNGG 7 cut(s) 77, 119, 571, 799, 1276, 2149, 2150
StyI CCWWGG 2 cut(s) 1093, 1980
TaaI ACNGT 9 cut(s) 525, 595, 633, 1447, 1673, 2211, 2231, 2325, 2597
TaiI ACGT 5 cut(s) 312, 663, 1501, 2176, 2398
TaqI TCGA 7 cut(s) 657, 1127, 1375, 1408, 1495, 2433, 2617
TaqII GACCGA 1 cut(s) 99
TatI WGTACW 3 cut(s) 1194, 1469, 2095
TfiI GAWTC 9 cut(s) 262, 404, 539, 1243, 2215, 2348, 2485, 2539, 2611
TscAI CASTG 4 cut(s) 1678, 2216, 2347, 2653
TseFI GTSAC 5 cut(s) 663, 1232, 1792, 1861, 2280
TseI GCWGC 5 cut(s) 177, 301, 1147, 1304, 2014
Tsp45I GTSAC 5 cut(s) 663, 1232, 1792, 1861, 2280
TspGWI ACGGA 1 cut(s) 1310
TspMI CCCGGG 1 cut(s) 2150
TspRI CASTG 4 cut(s) 1678, 2216, 2347, 2653
Van91I CCANNNNNTGG 1 cut(s) 217
Vha464I CTTAAG 3 cut(s) 710, 1778, 2456
VpaK11BI GGWCC 4 cut(s) 82, 208, 569, 625
VspI ATTAAT 1 cut(s) 1368
XapI RAATTY 7 cut(s) 287, 1339, 1491, 1619, 2140, 2357, 2561
XbaI TCTAGA 1 cut(s) 2474
XceI RCATGY 4 cut(s) 1162, 1207, 1352, 2554
XcmI CCANNNNNNNNNTGG 2 cut(s) 526, 881
XmaI CCCGGG 1 cut(s) 2150
XmiI GTMKAC 2 cut(s) 2022, 2203
XmnI GAANNNNTTC 1 cut(s) 2538
ZraI GACGTC 1 cut(s) 1499
ZrmI AGTACT 1 cut(s) 1471
Zsp2I ATGCAT 1 cut(s) 1164
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.