MD05G1207700.v1.1

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000865
Physical Location & Seq
Forward (+)
33866574 .. 33869789
3216 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1207700.v1.1.491

Sequence Viewer

Length: 2835 bp
ATGGCTGAAATTGCCATTCCACTAGCACTGGTGCTAATTGAAAATTTCAAGATTCCCAACCTCAATCTTTCAAGCGATGCCAGGGCTGATGTTAAAAGAGCCAAGACAATTTTGCGGAGGATGCGAGCCTATCTGACGGACTGCTCATCGAAACAGATCGGTGAAAGCTCCCAAGTGTTTCAAAATCATGCAAAAGAGGTCCAAGATGAAGTTTACGACATTGAGGATGCTCTTTCCATGTTTTTGGTTAAGATTCCTCATCACTACCATAGCAATTTAATCTCTAGATCTGCTCACAACGCTAAACATTACCCTTCTGAATGGATAGCACGCATACAGTTGTCGCGCATGCTCAAACAAATCGAAGAAAGGTTTCCACCAAATTTAGATTCACTTATTGACCGCCTACAAATTGCCAACACAGGATCTGAAGAAGATAATAATAATTTGGCTTATCACCAAGTTGTGGAAGAAGATGAAATTGTGGGGTTTGAGACGGCTATCACAAAACTCATCCAACAGTTGAGAGAAGAAGAATGTATCAGTCAAGGGCATCTCATGATTTCAATTGTTGGTCCTGGTGGCTCAGGCAAAACCACCCTAGTAAAAAATGTTTATGAGAGAAGAAGCGTGCAGGCTGCCTTTGATTGCCATGCTTTTGTTGAAGTGCCGCGCTCCTTTGAATTGCGAATGCTCTTGCTAACCATGCTGAGAAAATTTGAAGCCCAAAGTATGCAGGAACCTGTAGCTGAGCATCATCATCATCCTGAAGAGAAATTGAGACAAATTCTGGAGAAAAAGAAGTATTTGGTGGTTTTGGATAATGTTTGGAGCGAACAAGATTTGACACGTATAGTAAATGCTCTGCCAAAAGGTTTGTCTGGGAGTAAAATAATCACAACTACTCGGGACTCTAATATTGTAGCCTCTTGGCATACAAGTTCTGACGATTATATCCATGATTTGAGTAACGGATTATCCTCCAAAGAAGCCAATAAACTATTCTGCAAAAAGGCTTTTCACGGAAATTGGGACAACTGTCCAAAAGAGCTTGTGGAGTTGGCTCAAATAATCATCAGTGATTGTGGAGGCCTGCCCCTTGCAATTTCTACATTCGGTACTTTCCTAGCTTGGCAGAAGCCACGGAATTTTGTGAAGTGGAAGAAGTTCCATGATAGCTTTGGCTCCAATCTTCAGATTGTTAGTCAAGCTTGGGAGCCAAGTTATAGGGACCTGCCATACCATCTCAAAAGTTGTTTGTTGTACTTCAGCATGTTTCCAGAAGACAACTCTATCAAGCGTGAGAGGCTAATTCGCTTGTGGGTAGCCGAAGGATTTGTGAAGCAAGGAGGGAGAGCCACCATGGAGGAGGTTGCAGATGGTTATCTAAATCAACTCATCGGTAGAAAGTTGGTTGATGTGAGTTCAAGAGAGATCGATGGACAAGTAAGGAGTTGTCGTGTTTCCAACCTCGTTTGCAAGTTCATCATGTCAAAAGTGGAGGGTTTTGTCACTGTGTTGGAATCAAGCAACACTCGGGCAAGTACTAGCAGTACTAATTCAGGTGAGAAAATCCGACGCCTTTCTGCACATTATGTTTCTATAAAAGATTTATTAAGAAGAGGTGGAGAATTGAATCAAACACGTACCTTACTAGTGTTTGGCCCTTCCCAGCAGGCTCAATGTGATGAGGTTGGAAGGGTACTTAATACTCTTAAATATTTAAGGGTTTTAGATTTGAAAGGCATTCCTTTAGAGGACTTTCCAAAATCTATTTCTGGTCTCATTCTCTTAAAGTACATAAGTATGAGGAAAACCAAAATCAAAAGAGTCCCAAGTTCCATAAACCGGCTTGCTCATTTGGAAACTTTAGATCTTAAGCACACCCAGGTGACCAAATTACCAGAAGAGATTTATAAGCTCTACAATATGCGCCATCTTTTAGTGAGTCGTGGCTGTGATGGTGATGATGATGGACCCGCTCAAGGAGTAGAGATTTCTGCAGGCAACATTGGAGCTTTATCCGAATTACAAAAGTTGTCACTGATTAAGGTGGGAAACAACAGAACAATACTAAAAGCCTTAGCAGAACTAACTGGTCTCAGAAAGTTGGGCTTGACAGATCTCATAATTGAACATGGAACAGAGTTGTGTTGCGCTATTGAGAAAATGGGAAGTCTTTCAACACTTGAATTGAGATCAACAAACGAGGAGGAATATCTTGAGTTGGATCATCATATGGAATCCCCTCCTCGGTCTCTAGAACGCCTCAGTTTGAAAGGGAGGCTAAGATGTTTGCCGCAATGGGTTCCCATGCTTGATAGTGTAGTGAAGATTTCCCTAATGGGGTCAAAACTTGATGCTGATGCCAACCCACTCAAGCCCCTTCAAGCATTGCCGAATATGATGGAGCTTCATTTGGTTTGCTATTATACCGGACAAGTGTTAGAATTTGAGGCTAAAACGTTCATGAAGCTAAAGATATTAAGTATCAAACAATTTGATCAACTACATACCATGAAAGTTGAGTCTGGAGCAATGCCTAATCTTAAGAAGGTAACCCTGTCCAATTGTAAGAGTTTGAATTTACCTCCACTCGGCATGGAGGGTCTACGGGAACTCGAGGAACTGTTTCTCCATGACATGCCCACTGTACTCATTGCCAAGCTTCAAAGGGATGGTGAAGATCGTCAGGCGGTTGAGCATATTCCAGTCATCCATTCTTTTAATCTCAGGACTGGATTCCAAAATCTTTCTCAAGTGAGCAACAGCGCGGGGGTGAGAAGGGAGGAGAAACTGAAGGCGGCAATGGCTCGAAAAACTTCAACAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

945

Amino Acids

106.83

Weight (kDa)

8.54

Isoelectric Point (pI)

44.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 20 - 89 2.9e-09 Rx N-terminal domain
NB-ARC PF00931 165 - 343 1.1e-33 NB-ARC domain
WHD_DRP PF23559 425 - 495 1.4e-18 Disease resistance protein Winged helix domain
LRR_14 PF23598 549 - 877 5.1e-37 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1917
Acc36I ACCTGC 1 cut(s) 1242
AccB7I CCANNNNNTGG 1 cut(s) 466
AccBSI CCGCTC 1 cut(s) 1982
AccI GTMKAC 1 cut(s) 2611
AccII CGCG 3 cut(s) 346, 673, 2774
AciI CCGC 8 cut(s) 115, 403, 671, 1980, 2300, 2696, 2774, 2804
AclI AACGTT 1 cut(s) 2465
AclWI GGATC 2 cut(s) 433, 2238
AcsI RAATTY 7 cut(s) 43, 382, 716, 786, 1147, 2450, 2584
AcuI CTGAAG 5 cut(s) 450, 789, 1178, 1252, 2819
AcyI GRCGYC 1 cut(s) 1579
AfaI GTAC 8 cut(s) 1120, 1265, 1546, 1555, 1648, 1704, 1799, 2655
AfiI CCNNNNNNNGG 6 cut(s) 466, 1848, 1985, 2253, 2346, 2597
AflII CTTAAG 2 cut(s) 1877, 2549
AflIII ACRYGT 2 cut(s) 848, 1643
AhlI ACTAGT 1 cut(s) 1654
AjnI CCWGG 3 cut(s) 80, 577, 1887
AjuI GAANNNNNNNTTGG 6 cut(s) 373, 405, 986, 1018, 1759, 1791
AleI CACNNNNGTG 1 cut(s) 1889
AloI GAACNNNNNNTCC 2 cut(s) 2619, 2651
Alw26I GTCTC 5 cut(s) 488, 775, 1787, 2105, 2262
AlwI GGATC 2 cut(s) 433, 2238
AlwNI CAGNNNCTG 1 cut(s) 428
Ama87I CYCGRG 3 cut(s) 906, 1536, 2621
AoxI GGCC 2 cut(s) 1090, 1663
ApeKI GCWGC 1 cut(s) 638
ApoI RAATTY 7 cut(s) 43, 382, 716, 786, 1147, 2450, 2584
ArsI GACNNNNNNTTYG 2 cut(s) 863, 895
Asp700I GAANNNNTTC 5 cut(s) 372, 1166, 2179, 2631, 2821
AspLEI GCGC 5 cut(s) 348, 675, 1935, 2159, 2774
AspS9I GGNCC 5 cut(s) 199, 575, 1231, 1664, 1976
AsuHPI GGTGA 7 cut(s) 173, 449, 1577, 1903, 1976, 2693, 2791
AvaI CYCGRG 3 cut(s) 906, 1536, 2621
AvaII GGWCC 4 cut(s) 199, 575, 1231, 1976
BbsI GAAGAC 1 cut(s) 1290
BbvI GCAGC 1 cut(s) 625
BccI CCATC 8 cut(s) 1251, 1373, 1433, 1944, 1955, 1967, 2401, 2672
BceAI ACGGC 1 cut(s) 513
BciT130I CCWGG 3 cut(s) 82, 579, 1889
BclI TGATCA 1 cut(s) 2503
BcoDI GTCTC 5 cut(s) 488, 775, 1787, 2105, 2262
BcuI ACTAGT 1 cut(s) 1654
BfaI CTAG 7 cut(s) 23, 285, 602, 1127, 1548, 1655, 2261
BfmI CTRYAG 2 cut(s) 744, 2001
BfrI CTTAAG 2 cut(s) 1877, 2549
BfuAI ACCTGC 1 cut(s) 1242
BglII AGATCT 3 cut(s) 287, 1873, 2122
BisI GCNGC 4 cut(s) 639, 671, 2300, 2805
BlpI GCTNAGC 1 cut(s) 750
BlsI GCNGC 4 cut(s) 640, 672, 2301, 2806
BmcAI AGTACT 2 cut(s) 1546, 1555
Bme1390I CCNGG 3 cut(s) 82, 579, 1889
Bme18I GGWCC 4 cut(s) 199, 575, 1231, 1976
BmeT110I CYCGRG 3 cut(s) 906, 1536, 2621
BmgT120I GGNCC 5 cut(s) 199, 575, 1231, 1664, 1976
BmiI GGNNCC 6 cut(s) 741, 1186, 1218, 1232, 1978, 2310
BmrFI CCNGG 3 cut(s) 82, 579, 1889
BmsI GCATC 7 cut(s) 67, 111, 217, 562, 763, 2350, 2356
BoxI GACNNNNGTC 1 cut(s) 1038
BpiI GAAGAC 1 cut(s) 1290
BpmI CTGGAG 2 cut(s) 812, 2553
Bpu10I CCTNAGC 2 cut(s) 586, 2083
Bpu1102I GCTNAGC 1 cut(s) 750
BpuEI CTTGAG 4 cut(s) 1968, 2243, 2363, 2742
Bsa29I ATCGAT 1 cut(s) 1437
BsaAI YACGTR 2 cut(s) 851, 1646
BsaBI GATNNNNATC 1 cut(s) 1383
BsaHI GRCGYC 1 cut(s) 1579
BsaI GGTCTC 3 cut(s) 1787, 2105, 2262
BsaJI CCNNGG 5 cut(s) 81, 1142, 1362, 1887, 2252
BsaWI WCCGGW 1 cut(s) 2435
BsaXI ACNNNNNCTCC 4 cut(s) 1444, 1474, 2619, 2649
Bsc4I CCNNNNNNNGG 6 cut(s) 466, 1848, 1985, 2253, 2346, 2597
Bse118I RCCGGY 1 cut(s) 1848
Bse1I ACTGG 4 cut(s) 33, 2101, 2711, 2743
Bse3DI GCAATG 5 cut(s) 2309, 2393, 2544, 2658, 2814
Bse8I GATNNNNATC 1 cut(s) 1383
BseBI CCWGG 3 cut(s) 82, 579, 1889
BseCI ATCGAT 1 cut(s) 1437
BseDI CCNNGG 5 cut(s) 81, 1142, 1362, 1887, 2252
BseGI GGATG 6 cut(s) 126, 232, 513, 763, 2683, 2715
BseJI GATNNNNATC 1 cut(s) 1383
BseLI CCNNNNNNNGG 6 cut(s) 466, 1848, 1985, 2253, 2346, 2597
BseMI GCAATG 5 cut(s) 2309, 2393, 2544, 2658, 2814
BseMII CTCAG 6 cut(s) 600, 701, 741, 2116, 2284, 2746
BseNI ACTGG 4 cut(s) 33, 2101, 2711, 2743
BseRI GAGGAG 4 cut(s) 1382, 2225, 2241, 2804
BseXI GCAGC 1 cut(s) 625
BseYI CCCAGC 1 cut(s) 1671
BsgI GTGCAG 2 cut(s) 653, 1572
Bsh1236I CGCG 3 cut(s) 346, 673, 2774
BshFI GGCC 2 cut(s) 1092, 1665
BshVI ATCGAT 1 cut(s) 1437
BsiHKCI CYCGRG 3 cut(s) 906, 1536, 2621
BsiSI CCGG 2 cut(s) 1849, 2436
BslFI GGGAC 4 cut(s) 923, 1046, 1244, 1817
BslI CCNNNNNNNGG 6 cut(s) 466, 1848, 1985, 2253, 2346, 2597
BsmAI GTCTC 5 cut(s) 488, 775, 1787, 2105, 2262
BsmBI CGTCTC 1 cut(s) 488
BsmFI GGGAC 4 cut(s) 923, 1046, 1244, 1817
BsmI GAATGC 2 cut(s) 696, 1746
BsnI GGCC 2 cut(s) 1092, 1665
Bso31I GGTCTC 3 cut(s) 1787, 2105, 2262
BsoBI CYCGRG 3 cut(s) 906, 1536, 2621
Bsp1720I GCTNAGC 1 cut(s) 750
Bsp19I CCATGG 1 cut(s) 1362
BspACI CCGC 8 cut(s) 115, 403, 671, 1980, 2300, 2696, 2774, 2804
BspANI GGCC 2 cut(s) 1092, 1665
BspCNI CTCAG 6 cut(s) 599, 702, 742, 2115, 2283, 2745
BspDI ATCGAT 1 cut(s) 1437
BspFNI CGCG 3 cut(s) 346, 673, 2774
BspHI TCATGA 2 cut(s) 558, 2469
BspLI GGNNCC 6 cut(s) 741, 1186, 1218, 1232, 1978, 2310
BspMAI CTGCAG 1 cut(s) 2005
BspMI ACCTGC 1 cut(s) 1242
BspPI GGATC 2 cut(s) 433, 2238
BspTI CTTAAG 2 cut(s) 1877, 2549
BspTNI GGTCTC 3 cut(s) 1787, 2105, 2262
BsrBI CCGCTC 1 cut(s) 1982
BsrDI GCAATG 5 cut(s) 2309, 2393, 2544, 2658, 2814
BsrFI RCCGGY 1 cut(s) 1848
BsrI ACTGG 4 cut(s) 33, 2101, 2711, 2743
BssAI RCCGGY 1 cut(s) 1848
BssECI CCNNGG 5 cut(s) 81, 1142, 1362, 1887, 2252
BssNI GRCGYC 1 cut(s) 1579
BssT1I CCWWGG 1 cut(s) 1362
Bst2UI CCWGG 3 cut(s) 82, 579, 1889
Bst4CI ACNGT 6 cut(s) 339, 522, 1040, 1516, 2631, 2653
Bst6I CTCTTC 3 cut(s) 765, 1615, 1902
BstACI GRCGYC 1 cut(s) 1579
BstAFI CTTAAG 2 cut(s) 1877, 2549
BstBAI YACGTR 2 cut(s) 851, 1646
BstC8I GCNNGC 9 cut(s) 126, 331, 350, 632, 636, 1094, 1677, 1854, 2005
BstDEI CTNAG 8 cut(s) 586, 710, 750, 2083, 2102, 2270, 2288, 2732
BstDSI CCRYGG 2 cut(s) 1142, 1362
BstEII GGTNACC 2 cut(s) 1891, 2557
BstF5I GGATG 6 cut(s) 126, 232, 513, 763, 2683, 2715
BstFNI CGCG 3 cut(s) 346, 673, 2774
BstHHI GCGC 5 cut(s) 348, 675, 1935, 2159, 2774
BstMAI GTCTC 5 cut(s) 488, 775, 1787, 2105, 2262
BstMWI GCNNNNNNNGC 6 cut(s) 11, 121, 299, 706, 1306, 2810
BstNI CCWGG 3 cut(s) 82, 579, 1889
BstNSI RCATGY 3 cut(s) 352, 1276, 2647
BstPAI GACNNNNGTC 1 cut(s) 1038
BstPI GGTNACC 2 cut(s) 1891, 2557
BstSCI CCNGG 3 cut(s) 80, 577, 1887
BstSFI CTRYAG 2 cut(s) 744, 2001
BstUI CGCG 3 cut(s) 346, 673, 2774
BstV1I GCAGC 1 cut(s) 625
BstV2I GAAGAC 1 cut(s) 1290
BstX2I RGATCY 4 cut(s) 287, 425, 1873, 2122
BstXI CCANNNNNNTGG 1 cut(s) 244
BstYI RGATCY 4 cut(s) 287, 425, 1873, 2122
Bsu15I ATCGAT 1 cut(s) 1437
BsuRI GGCC 2 cut(s) 1092, 1665
BsuTUI ATCGAT 1 cut(s) 1437
BtgI CCRYGG 2 cut(s) 1142, 1362
BtgZI GCGATG 1 cut(s) 90
BtsCI GGATG 6 cut(s) 126, 232, 513, 763, 2683, 2715
BtsIMutI CAGTG 5 cut(s) 26, 1084, 1512, 2042, 2649
BveI ACCTGC 1 cut(s) 1242
Cac8I GCNNGC 9 cut(s) 126, 331, 350, 632, 636, 1094, 1677, 1854, 2005
CaiI CAGNNNCTG 1 cut(s) 428
CciI TCATGA 2 cut(s) 558, 2469
CfoI GCGC 5 cut(s) 348, 675, 1935, 2159, 2774
Cfr10I RCCGGY 1 cut(s) 1848
Cfr13I GGNCC 5 cut(s) 199, 575, 1231, 1664, 1976
ClaI ATCGAT 1 cut(s) 1437
CseI GACGC 1 cut(s) 1587
Csp6I GTAC 8 cut(s) 1119, 1264, 1545, 1554, 1647, 1703, 1798, 2654
CviQI GTAC 8 cut(s) 1119, 1264, 1545, 1554, 1647, 1703, 1798, 2654
DdeI CTNAG 8 cut(s) 586, 710, 750, 2083, 2102, 2270, 2288, 2732
Eam1104I CTCTTC 3 cut(s) 765, 1615, 1902
EarI CTCTTC 3 cut(s) 765, 1615, 1902
Eco130I CCWWGG 1 cut(s) 1362
Eco147I AGGCCT 1 cut(s) 1092
Eco31I GGTCTC 3 cut(s) 1787, 2105, 2262
Eco47I GGWCC 4 cut(s) 199, 575, 1231, 1976
Eco57I CTGAAG 5 cut(s) 450, 789, 1178, 1252, 2819
Eco88I CYCGRG 3 cut(s) 906, 1536, 2621
Eco91I GGTNACC 2 cut(s) 1891, 2557
EcoO109I RGGNCCY 1 cut(s) 1231
EcoO65I GGTNACC 2 cut(s) 1891, 2557
EcoRII CCWGG 3 cut(s) 80, 577, 1887
EcoT14I CCWWGG 1 cut(s) 1362
ErhI CCWWGG 1 cut(s) 1362
Esp3I CGTCTC 1 cut(s) 488
FalI AAGNNNNNCTT 2 cut(s) 2099, 2131
FaqI GGGAC 4 cut(s) 923, 1046, 1244, 1817
FauI CCCGC 2 cut(s) 1987, 2767
FauNDI CATATG 1 cut(s) 2238
FbaI TGATCA 1 cut(s) 2503
FblI GTMKAC 1 cut(s) 2611
Fnu4HI GCNGC 4 cut(s) 639, 671, 2300, 2805
FokI GGATG 6 cut(s) 133, 239, 500, 750, 2690, 2702
Fsp4HI GCNGC 4 cut(s) 639, 671, 2300, 2805
FspBI CTAG 7 cut(s) 23, 285, 602, 1127, 1548, 1655, 2261
GlaI GCGC 5 cut(s) 347, 674, 1934, 2158, 2773
GluI GCNGC 4 cut(s) 639, 671, 2300, 2805
GsaI CCCAGC 1 cut(s) 1675
GsuI CTGGAG 2 cut(s) 812, 2553
HaeIII GGCC 2 cut(s) 1092, 1665
HapII CCGG 2 cut(s) 1849, 2436
HgaI GACGC 1 cut(s) 1587
HhaI GCGC 5 cut(s) 348, 675, 1935, 2159, 2774
Hin1I GRCGYC 1 cut(s) 1579
Hin6I GCGC 5 cut(s) 346, 673, 1933, 2157, 2772
HinP1I GCGC 5 cut(s) 346, 673, 1933, 2157, 2772
HindIII AAGCTT 2 cut(s) 1209, 2666
HpaII CCGG 2 cut(s) 1849, 2436
HphI GGTGA 7 cut(s) 173, 449, 1577, 1903, 1976, 2693, 2791
Hpy166II GTNNAC 2 cut(s) 214, 2612
Hpy188I TCNGA 8 cut(s) 135, 319, 430, 946, 1197, 1577, 2026, 2105
Hpy8I GTNNAC 2 cut(s) 214, 2612
Hpy99I CGWCG 1 cut(s) 1581
HpyAV CCTTC 8 cut(s) 324, 1325, 1677, 1692, 2396, 2548, 2778, 2794
HpyCH4III ACNGT 6 cut(s) 339, 522, 1040, 1516, 2631, 2653
HpyCH4IV ACGT 3 cut(s) 850, 1645, 2465
HpyCH4V TGCA 9 cut(s) 191, 634, 736, 1008, 1103, 1376, 1479, 1589, 2003
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 121, 299, 706, 1306, 2810
HpyF3I CTNAG 8 cut(s) 586, 710, 750, 2083, 2102, 2270, 2288, 2732
HpySE526I ACGT 3 cut(s) 850, 1645, 2465
Hsp92I GRCGYC 1 cut(s) 1579
HspAI GCGC 5 cut(s) 346, 673, 1933, 2157, 2772
Ksp22I TGATCA 1 cut(s) 2503
LmnI GCTCC 8 cut(s) 173, 680, 831, 1190, 1216, 2015, 2410, 2534
Lsp1109I GCAGC 1 cut(s) 625
LweI GCATC 7 cut(s) 67, 111, 217, 562, 763, 2350, 2356
MaeI CTAG 7 cut(s) 23, 285, 602, 1127, 1548, 1655, 2261
MaeII ACGT 3 cut(s) 850, 1645, 2465
MaeIII GTNAC 5 cut(s) 968, 1510, 1891, 2040, 2557
MbiI CCGCTC 1 cut(s) 1982
MfeI CAATTG 2 cut(s) 567, 2569
MflI RGATCY 4 cut(s) 287, 425, 1873, 2122
MlyI GAGTC 4 cut(s) 905, 1839, 1957, 2537
MmeI TCCRAC 6 cut(s) 541, 1491, 1500, 1600, 1675, 2208
MroXI GAANNNNTTC 5 cut(s) 372, 1166, 2179, 2631, 2821
MslI CAYNNNNRTG 2 cut(s) 1805, 1889
MspCI CTTAAG 2 cut(s) 1877, 2549
MspI CCGG 2 cut(s) 1849, 2436
MspR9I CCNGG 3 cut(s) 82, 579, 1889
MunI CAATTG 2 cut(s) 567, 2569
Mva1269I GAATGC 2 cut(s) 696, 1746
MvaI CCWGG 3 cut(s) 82, 579, 1889
MvnI CGCG 3 cut(s) 346, 673, 2774
MwoI GCNNNNNNNGC 6 cut(s) 11, 121, 299, 706, 1306, 2810
NcoI CCATGG 1 cut(s) 1362
NdeI CATATG 1 cut(s) 2238
NlaIV GGNNCC 6 cut(s) 741, 1186, 1218, 1232, 1978, 2310
NmeAIII GCCGAG 1 cut(s) 2577
NmuCI GTSAC 3 cut(s) 1510, 1891, 2040
NspI RCATGY 3 cut(s) 352, 1276, 2647
OliI CACNNNNGTG 1 cut(s) 1889
PaeI GCATGC 1 cut(s) 352
PaeR7I CTCGAG 1 cut(s) 2621
PagI TCATGA 2 cut(s) 558, 2469
PceI AGGCCT 1 cut(s) 1092
PctI GAATGC 2 cut(s) 696, 1746
PdmI GAANNNNTTC 5 cut(s) 372, 1166, 2179, 2631, 2821
PfeI GAWTC 7 cut(s) 52, 253, 389, 1523, 1636, 2243, 2742
PflMI CCANNNNNTGG 1 cut(s) 466
PkrI GCNGC 4 cut(s) 640, 672, 2301, 2806
PleI GAGTC 4 cut(s) 905, 1838, 1956, 2536
PpsI GAGTC 4 cut(s) 905, 1838, 1956, 2536
Ppu21I YACGTR 2 cut(s) 851, 1646
PpuMI RGGWCCY 1 cut(s) 1231
PshAI GACNNNNGTC 1 cut(s) 1038
PsiI TTATAA 1 cut(s) 1917
Psp1406I AACGTT 1 cut(s) 2465
Psp5II RGGWCCY 1 cut(s) 1231
Psp6I CCWGG 3 cut(s) 80, 577, 1887
PspEI GGTNACC 2 cut(s) 1891, 2557
PspFI CCCAGC 1 cut(s) 1671
PspGI CCWGG 3 cut(s) 80, 577, 1887
PspN4I GGNNCC 6 cut(s) 741, 1186, 1218, 1232, 1978, 2310
PspPI GGNCC 5 cut(s) 199, 575, 1231, 1664, 1976
PspPPI RGGWCCY 1 cut(s) 1231
PspXI VCTCGAGB 1 cut(s) 2621
PstI CTGCAG 1 cut(s) 2005
PstNI CAGNNNCTG 1 cut(s) 428
PsuI RGATCY 4 cut(s) 287, 425, 1873, 2122
RsaI GTAC 8 cut(s) 1120, 1265, 1546, 1555, 1648, 1704, 1799, 2655
RsaNI GTAC 8 cut(s) 1119, 1264, 1545, 1554, 1647, 1703, 1798, 2654
RseI CAYNNNNRTG 2 cut(s) 1805, 1889
SatI GCNGC 4 cut(s) 639, 671, 2300, 2805
Sau96I GGNCC 5 cut(s) 199, 575, 1231, 1664, 1976
ScaI AGTACT 2 cut(s) 1546, 1555
SchI GAGTC 4 cut(s) 905, 1839, 1957, 2537
ScrFI CCNGG 3 cut(s) 82, 579, 1889
SfaNI GCATC 7 cut(s) 67, 111, 217, 562, 763, 2350, 2356
SfcI CTRYAG 2 cut(s) 744, 2001
Sfr274I CTCGAG 1 cut(s) 2621
SinI GGWCC 4 cut(s) 199, 575, 1231, 1976
SlaI CTCGAG 1 cut(s) 2621
SmiMI CAYNNNNRTG 2 cut(s) 1805, 1889
SmlI CTYRAG 7 cut(s) 1877, 1983, 2222, 2378, 2549, 2621, 2757
SmoI CTYRAG 7 cut(s) 1877, 1983, 2222, 2378, 2549, 2621, 2757
SpeI ACTAGT 1 cut(s) 1654
SphI GCATGC 1 cut(s) 352
SseBI AGGCCT 1 cut(s) 1092
SsiI CCGC 8 cut(s) 115, 403, 671, 1980, 2300, 2696, 2774, 2804
SspI AATATT 2 cut(s) 919, 1721
SspMI CTAG 7 cut(s) 23, 285, 602, 1127, 1548, 1655, 2261
StuI AGGCCT 1 cut(s) 1092
StyD4I CCNGG 3 cut(s) 80, 577, 1887
StyI CCWWGG 1 cut(s) 1362
TaaI ACNGT 6 cut(s) 339, 522, 1040, 1516, 2631, 2653
TaiI ACGT 3 cut(s) 853, 1648, 2468
TaqI TCGA 5 cut(s) 149, 363, 1437, 2622, 2815
TaqII GACCGA 1 cut(s) 2244
TatI WGTACW 5 cut(s) 1263, 1544, 1553, 1797, 2653
TauI GCSGC 3 cut(s) 673, 2302, 2807
TfiI GAWTC 7 cut(s) 52, 253, 389, 1523, 1636, 2243, 2742
TscAI CASTG 5 cut(s) 33, 1084, 1519, 2049, 2656
TseFI GTSAC 3 cut(s) 1510, 1891, 2040
TseI GCWGC 1 cut(s) 638
Tsp45I GTSAC 3 cut(s) 1510, 1891, 2040
TspDTI ATGAA 7 cut(s) 222, 492, 1474, 2405, 2458, 2486, 2534
TspGWI ACGGA 4 cut(s) 152, 987, 1038, 1159
TspRI CASTG 5 cut(s) 33, 1084, 1519, 2049, 2656
Van91I CCANNNNNTGG 1 cut(s) 466
Vha464I CTTAAG 2 cut(s) 1877, 2549
VpaK11BI GGWCC 4 cut(s) 199, 575, 1231, 1976
XapI RAATTY 7 cut(s) 43, 382, 716, 786, 1147, 2450, 2584
XbaI TCTAGA 2 cut(s) 284, 2260
XceI RCATGY 3 cut(s) 352, 1276, 2647
XcmI CCANNNNNNNNNTGG 1 cut(s) 1178
XhoI CTCGAG 1 cut(s) 2621
XmiI GTMKAC 1 cut(s) 2611
XmnI GAANNNNTTC 5 cut(s) 372, 1166, 2179, 2631, 2821
XspI CTAG 7 cut(s) 23, 285, 602, 1127, 1548, 1655, 2261
ZrmI AGTACT 2 cut(s) 1546, 1555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.