Rh5AG196400

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000865
Physical Location & Seq
Forward (+)
22936908 .. 22939721
2814 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG196400.1

Sequence Viewer

Length: 1221 bp
ATGGCTGAACTTGCCATTCCTGTAGCACTGGCCTTAATTGACAAGGTGATAATCATCCTCCAGCAAAACTTTCACCGTCAAACTAGAGTCAAGGATGATGTTGAAAAAGCCAAGAATTGGTTGAGGAGGATGGAAGCGTATCTAGCCGACACATCAGAACATAGCGATGAGGGTACTCGAGTGTTTCAGACTCGAGTAAGAGAAATCCAAGATTTGGTTTATGAAATTGAGGATGCTCTCGACAAGTTCATGCTTCAGGTTCCTCATCACATCCATAGCAATATAGCCTCTCAGACGGTACACAAAGCTGTTCATTATTTGCCAACCAGGAATGCAGGCAAGGAGTTGTCGACTGAGATTAATGGTATCGAGAAAAAAGTTCGTTGGATTACAGAACTAGATGCTCTTCATATCCAAGCTCCAGCACAAAGCCGCCCAAGTTCCAGTTCCGGGGAAGGAATGACCCACTACTACCATGTTCTTGAAGAAGAAGAAGAAATAGTGGGCTTCGAGGAGCATGAAGAAAAACTCTTGGCACAGTTGATGGAAGAAAATTCAAGTCTTTTGACGATTTCGATTGTAGGCCCGGGCGGTTCCGGCAAAACCACTCTGGCAAAGAAGGTTTTCGACAACAAGCGGGTGCAAGAGTTTTTTGACTGCCAAGCTTGGGTTGATGTGCCACGCGCATTTGTACCCAAAGAGGTTTTGCCAAACATTCTAAGCAAAATTGAGGCAAAAGGGCAGAAGGAACCAGTAATTCATGGAGAAAATCCAAAGGATAAATTGAGACGACTTCTCAAGGACAAGCGGTTTTTGGTCGTTTTGGATAATGTTTGGAACAGAACAAATTTGGAATGCGTCGTAGATGCATTACAACTTGGTTTTAATGGCAGTAGAATCATCATCACTACTCGTAATGGAGCTTCTATTTCCTCTGAGCCATATGTCCTTGATTTGAGTGATGGTTTACCTAGGGAAAAAGCCGAGTACCTATTCTGCAAGAAGTTACTTCCAGCATTTGGCCCTGATGATCTGAAATCACTGGAGGAACTGCTTCTGTATGACATGCACAAGGAATTCATTGCCAAGCTTCAGAATGATTCCGAAGACCGTCTGGGGTATAAGCATGTTAGAGTCATACATTCTTTCAGTCTCGGCAGCAACCATTCTTTCACTGATTTCAAGAATCTTTCTCCATCACAGTGGCTTGTGGAACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

406

Amino Acids

46.39

Weight (kDa)

5.89

Isoelectric Point (pI)

55.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 11 - 93 3.1e-14 Rx N-terminal domain
NB-ARC PF00931 171 - 308 2.3e-30 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 117, 214, 1019
AccI GTMKAC 1 cut(s) 350
AccII CGCG 1 cut(s) 684
AciI CCGC 4 cut(s) 433, 591, 637, 808
AcsI RAATTY 3 cut(s) 553, 847, 1076
AcuI CTGAAG 2 cut(s) 239, 1076
AfaI GTAC 4 cut(s) 175, 300, 693, 989
AfiI CCNNNNNNNGG 5 cut(s) 117, 214, 450, 667, 1019
AgsI TTSAA 4 cut(s) 104, 485, 558, 1183
AjnI CCWGG 1 cut(s) 326
AjuI GAANNNNNNNTTGG 2 cut(s) 430, 462
AluBI AGCT 5 cut(s) 308, 419, 665, 923, 1090
AluI AGCT 5 cut(s) 308, 419, 665, 923, 1090
Alw26I GTCTC 2 cut(s) 781, 1157
Ama87I CYCGRG 3 cut(s) 177, 192, 586
AoxI GGCC 3 cut(s) 30, 583, 1021
ApeKI GCWGC 1 cut(s) 1158
ApoI RAATTY 3 cut(s) 553, 847, 1076
AseI ATTAAT 1 cut(s) 360
Asp700I GAANNNNTTC 2 cut(s) 623, 1053
AspA2I CCTAGG 1 cut(s) 971
AspLEI GCGC 1 cut(s) 686
AspS9I GGNCC 2 cut(s) 584, 1022
AsuC2I CCSGG 3 cut(s) 451, 587, 588
AsuHPI GGTGA 2 cut(s) 58, 65
AvaI CYCGRG 3 cut(s) 177, 192, 586
AvrII CCTAGG 1 cut(s) 971
BaeI ACNNNNGTAYC 2 cut(s) 165, 198
BarI GAAGNNNNNNTAC 2 cut(s) 907, 939
BbsI GAAGAC 1 cut(s) 1113
BbvI GCAGC 1 cut(s) 1170
BccI CCATC 4 cut(s) 124, 538, 956, 1204
BciT130I CCWGG 1 cut(s) 328
BcnI CCSGG 3 cut(s) 451, 587, 588
BcoDI GTCTC 2 cut(s) 781, 1157
BfaI CTAG 4 cut(s) 84, 143, 398, 972
BfmI CTRYAG 1 cut(s) 21
BisI GCNGC 2 cut(s) 433, 1159
BlnI CCTAGG 1 cut(s) 971
BlsI GCNGC 2 cut(s) 434, 1160
Bme1390I CCNGG 4 cut(s) 328, 451, 587, 588
BmeT110I CYCGRG 3 cut(s) 177, 192, 586
BmgT120I GGNCC 2 cut(s) 584, 1022
BmiI GGNNCC 3 cut(s) 261, 595, 750
BmrFI CCNGG 4 cut(s) 328, 451, 587, 588
BmsI GCATC 3 cut(s) 223, 391, 856
BpiI GAAGAC 1 cut(s) 1113
BpmI CTGGAG 3 cut(s) 44, 405, 1064
BpuEI CTTGAG 1 cut(s) 782
BpuMI CCSGG 3 cut(s) 451, 587, 588
BsaBI GATNNNNATC 1 cut(s) 53
BsaJI CCNNGG 3 cut(s) 450, 586, 971
Bsc4I CCNNNNNNNGG 5 cut(s) 117, 214, 450, 667, 1019
Bse1I ACTGG 4 cut(s) 33, 444, 752, 1047
Bse3DI GCAATG 1 cut(s) 1080
Bse8I GATNNNNATC 1 cut(s) 53
BseBI CCWGG 1 cut(s) 328
BseDI CCNNGG 3 cut(s) 450, 586, 971
BseGI GGATG 5 cut(s) 54, 100, 135, 238, 270
BseJI GATNNNNATC 1 cut(s) 53
BseLI CCNNNNNNNGG 5 cut(s) 117, 214, 450, 667, 1019
BseMI GCAATG 1 cut(s) 1080
BseMII CTCAG 3 cut(s) 305, 345, 927
BseNI ACTGG 4 cut(s) 33, 444, 752, 1047
BseRI GAGGAG 2 cut(s) 139, 527
BseXI GCAGC 1 cut(s) 1170
Bsh1236I CGCG 1 cut(s) 684
BshFI GGCC 3 cut(s) 32, 585, 1023
BsiHKCI CYCGRG 3 cut(s) 177, 192, 586
BsiSI CCGG 3 cut(s) 450, 587, 597
BslI CCNNNNNNNGG 5 cut(s) 117, 214, 450, 667, 1019
BsmAI GTCTC 2 cut(s) 781, 1157
BsmBI CGTCTC 1 cut(s) 781
BsmI GAATGC 2 cut(s) 337, 860
BsnI GGCC 3 cut(s) 32, 585, 1023
BsoBI CYCGRG 3 cut(s) 177, 192, 586
Bsp143I GATC 1 cut(s) 1030
BspACI CCGC 4 cut(s) 433, 591, 637, 808
BspANI GGCC 3 cut(s) 32, 585, 1023
BspCNI CTCAG 3 cut(s) 304, 346, 928
BspFNI CGCG 1 cut(s) 684
BspLI GGNNCC 3 cut(s) 261, 595, 750
BspQI GCTCTTC 1 cut(s) 411
BsrDI GCAATG 1 cut(s) 1080
BsrI ACTGG 4 cut(s) 33, 444, 752, 1047
BssECI CCNNGG 3 cut(s) 450, 586, 971
BssMI GATC 1 cut(s) 1030
BssT1I CCWWGG 1 cut(s) 971
Bst2UI CCWGG 1 cut(s) 328
Bst4CI ACNGT 5 cut(s) 77, 298, 540, 1112, 1203
Bst6I CTCTTC 1 cut(s) 411
BstC8I GCNNGC 1 cut(s) 337
BstDEI CTNAG 4 cut(s) 291, 354, 719, 936
BstF5I GGATG 5 cut(s) 54, 100, 135, 238, 270
BstFNI CGCG 1 cut(s) 684
BstHHI GCGC 1 cut(s) 686
BstKTI GATC 1 cut(s) 1033
BstMAI GTCTC 2 cut(s) 781, 1157
BstMBI GATC 1 cut(s) 1030
BstMWI GCNNNNNNNGC 3 cut(s) 11, 143, 597
BstNI CCWGG 1 cut(s) 328
BstNSI RCATGY 2 cut(s) 1069, 1130
BstSCI CCNGG 4 cut(s) 326, 449, 585, 586
BstSFI CTRYAG 1 cut(s) 21
BstUI CGCG 1 cut(s) 684
BstV1I GCAGC 1 cut(s) 1170
BstV2I GAAGAC 1 cut(s) 1113
BstXI CCANNNNNNTGG 1 cut(s) 1203
BsuRI GGCC 3 cut(s) 32, 585, 1023
BtgZI GCGATG 1 cut(s) 180
BtsCI GGATG 5 cut(s) 54, 100, 135, 238, 270
BtsIMutI CAGTG 4 cut(s) 26, 1040, 1173, 1208
Cac8I GCNNGC 1 cut(s) 337
CfoI GCGC 1 cut(s) 686
Cfr13I GGNCC 2 cut(s) 584, 1022
Cfr9I CCCGGG 1 cut(s) 586
CseI GACGC 1 cut(s) 847
Csp6I GTAC 4 cut(s) 174, 299, 692, 988
CviAII CATG 6 cut(s) 250, 476, 518, 761, 1066, 1127
CviQI GTAC 4 cut(s) 174, 299, 692, 988
DdeI CTNAG 4 cut(s) 291, 354, 719, 936
DpnI GATC 1 cut(s) 1032
DpnII GATC 1 cut(s) 1030
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
Eco130I CCWWGG 1 cut(s) 971
Eco57I CTGAAG 2 cut(s) 239, 1076
Eco88I CYCGRG 3 cut(s) 177, 192, 586
EcoRI GAATTC 1 cut(s) 1076
EcoRII CCWGG 1 cut(s) 326
EcoT14I CCWWGG 1 cut(s) 971
EcoT22I ATGCAT 1 cut(s) 871
ErhI CCWWGG 1 cut(s) 971
Esp3I CGTCTC 1 cut(s) 781
FaeI CATG 6 cut(s) 253, 479, 521, 764, 1069, 1130
FatI CATG 6 cut(s) 249, 475, 517, 760, 1065, 1126
FauI CCCGC 1 cut(s) 630
FauNDI CATATG 1 cut(s) 943
FblI GTMKAC 1 cut(s) 350
Fnu4HI GCNGC 2 cut(s) 433, 1159
FokI GGATG 5 cut(s) 41, 107, 142, 245, 257
Fsp4HI GCNGC 2 cut(s) 433, 1159
FspBI CTAG 4 cut(s) 84, 143, 398, 972
GlaI GCGC 1 cut(s) 685
GluI GCNGC 2 cut(s) 433, 1159
GsuI CTGGAG 3 cut(s) 44, 405, 1064
HaeIII GGCC 3 cut(s) 32, 585, 1023
HapII CCGG 3 cut(s) 450, 587, 597
HgaI GACGC 1 cut(s) 847
HhaI GCGC 1 cut(s) 686
Hin1II CATG 6 cut(s) 253, 479, 521, 764, 1069, 1130
Hin6I GCGC 1 cut(s) 684
HinP1I GCGC 1 cut(s) 684
HincII GTYRAC 1 cut(s) 351
HindII GTYRAC 1 cut(s) 351
HindIII AAGCTT 2 cut(s) 663, 1088
HinfI GANTC 6 cut(s) 87, 190, 897, 1100, 1134, 1186
HpaII CCGG 3 cut(s) 450, 587, 597
HphI GGTGA 2 cut(s) 58, 65
Hpy166II GTNNAC 3 cut(s) 301, 351, 968
Hpy188I TCNGA 7 cut(s) 157, 189, 294, 937, 1035, 1095, 1105
Hpy188III TCNNGA 4 cut(s) 239, 370, 482, 1183
Hpy8I GTNNAC 3 cut(s) 301, 351, 968
Hpy99I CGWCG 1 cut(s) 863
HpyAV CCTTC 3 cut(s) 449, 613, 739
HpyCH4III ACNGT 5 cut(s) 77, 298, 540, 1112, 1203
HpyCH4V TGCA 5 cut(s) 335, 643, 869, 999, 1069
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 143, 597
HpyF3I CTNAG 4 cut(s) 291, 354, 719, 936
Hsp92II CATG 6 cut(s) 253, 479, 521, 764, 1069, 1130
HspAI GCGC 1 cut(s) 684
Kzo9I GATC 1 cut(s) 1030
LguI GCTCTTC 1 cut(s) 411
LmnI GCTCC 3 cut(s) 424, 514, 920
Lsp1109I GCAGC 1 cut(s) 1170
LweI GCATC 3 cut(s) 223, 391, 856
MaeI CTAG 4 cut(s) 84, 143, 398, 972
MaeIII GTNAC 1 cut(s) 1005
MalI GATC 1 cut(s) 1032
MboI GATC 1 cut(s) 1030
MboII GAAGA 8 cut(s) 398, 497, 500, 503, 506, 533, 560, 1118
MluCI AATT 9 cut(s) 36, 115, 225, 553, 726, 756, 782, 847, 1076
MlyI GAGTC 3 cut(s) 96, 184, 1143
MmeI TCCRAC 1 cut(s) 365
Mph1103I ATGCAT 1 cut(s) 871
MroXI GAANNNNTTC 2 cut(s) 623, 1053
MseI TTAA 3 cut(s) 35, 360, 885
MslI CAYNNNNRTG 2 cut(s) 165, 1201
MspI CCGG 3 cut(s) 450, 587, 597
MspR9I CCNGG 4 cut(s) 328, 451, 587, 588
Mva1269I GAATGC 2 cut(s) 337, 860
MvaI CCWGG 1 cut(s) 328
MvnI CGCG 1 cut(s) 684
MwoI GCNNNNNNNGC 3 cut(s) 11, 143, 597
NciI CCSGG 3 cut(s) 451, 587, 588
NdeI CATATG 1 cut(s) 943
NdeII GATC 1 cut(s) 1030
NlaIII CATG 6 cut(s) 253, 479, 521, 764, 1069, 1130
NlaIV GGNNCC 3 cut(s) 261, 595, 750
NmeAIII GCCGAG 2 cut(s) 1009, 1134
NsiI ATGCAT 1 cut(s) 871
NspI RCATGY 2 cut(s) 1069, 1130
PaeR7I CTCGAG 2 cut(s) 177, 192
PciSI GCTCTTC 1 cut(s) 411
PctI GAATGC 2 cut(s) 337, 860
PdmI GAANNNNTTC 2 cut(s) 623, 1053
PfeI GAWTC 3 cut(s) 897, 1100, 1186
PflMI CCANNNNNTGG 3 cut(s) 117, 214, 1019
PkrI GCNGC 2 cut(s) 434, 1160
PleI GAGTC 3 cut(s) 95, 184, 1142
PpsI GAGTC 3 cut(s) 95, 184, 1142
PshBI ATTAAT 1 cut(s) 360
Psp6I CCWGG 1 cut(s) 326
PspGI CCWGG 1 cut(s) 326
PspN4I GGNNCC 3 cut(s) 261, 595, 750
PspPI GGNCC 2 cut(s) 584, 1022
PspXI VCTCGAGB 2 cut(s) 177, 192
RsaI GTAC 4 cut(s) 175, 300, 693, 989
RsaNI GTAC 4 cut(s) 174, 299, 692, 988
RseI CAYNNNNRTG 2 cut(s) 165, 1201
SalI GTCGAC 1 cut(s) 349
SapI GCTCTTC 1 cut(s) 411
SaqAI TTAA 3 cut(s) 35, 360, 885
SatI GCNGC 2 cut(s) 433, 1159
Sau3AI GATC 1 cut(s) 1030
Sau96I GGNCC 2 cut(s) 584, 1022
SchI GAGTC 3 cut(s) 96, 184, 1143
ScrFI CCNGG 4 cut(s) 328, 451, 587, 588
SfaNI GCATC 3 cut(s) 223, 391, 856
SfcI CTRYAG 1 cut(s) 21
Sfr274I CTCGAG 2 cut(s) 177, 192
SlaI CTCGAG 2 cut(s) 177, 192
SmaI CCCGGG 1 cut(s) 588
SmiMI CAYNNNNRTG 2 cut(s) 165, 1201
SmlI CTYRAG 3 cut(s) 177, 192, 797
SmoI CTYRAG 3 cut(s) 177, 192, 797
SrfI GCCCGGGC 1 cut(s) 588
Sse9I AATT 9 cut(s) 36, 115, 225, 553, 726, 756, 782, 847, 1076
SsiI CCGC 4 cut(s) 433, 591, 637, 808
SspMI CTAG 4 cut(s) 84, 143, 398, 972
StyD4I CCNGG 4 cut(s) 326, 449, 585, 586
StyI CCWWGG 1 cut(s) 971
TaaI ACNGT 5 cut(s) 77, 298, 540, 1112, 1203
TaqI TCGA 8 cut(s) 178, 193, 240, 350, 369, 510, 575, 627
TasI AATT 9 cut(s) 36, 115, 225, 553, 726, 756, 782, 847, 1076
TauI GCSGC 1 cut(s) 435
TfiI GAWTC 3 cut(s) 897, 1100, 1186
Tru1I TTAA 3 cut(s) 35, 360, 885
Tru9I TTAA 3 cut(s) 35, 360, 885
TscAI CASTG 4 cut(s) 33, 1047, 1180, 1208
TseI GCWGC 1 cut(s) 1158
TspDTI ATGAA 7 cut(s) 237, 238, 302, 398, 534, 749, 1069
TspMI CCCGGG 1 cut(s) 586
TspRI CASTG 4 cut(s) 33, 1047, 1180, 1208
Van91I CCANNNNNTGG 3 cut(s) 117, 214, 1019
VspI ATTAAT 1 cut(s) 360
XapI RAATTY 3 cut(s) 553, 847, 1076
XceI RCATGY 2 cut(s) 1069, 1130
XhoI CTCGAG 2 cut(s) 177, 192
XmaI CCCGGG 1 cut(s) 586
XmaJI CCTAGG 1 cut(s) 971
XmiI GTMKAC 1 cut(s) 350
XmnI GAANNNNTTC 2 cut(s) 623, 1053
XspI CTAG 4 cut(s) 84, 143, 398, 972
Zsp2I ATGCAT 1 cut(s) 871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.