RchiOBHm_Chr2g0167971

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000865
Physical Location & Seq
Reverse (-)
82475850 .. 82478591
2742 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53572

Sequence Viewer

Length: 2742 bp
ATGGCTGATGCTATTTCAGCCGCATTGCTGTTAGTTGAGACGGCGATAATCTGCACCGACCAATTACACAGTCGTACGAGTAGTGTCCGTAAGGATCTTGAAAGGGCCCGGAATTGTTTGGCGAGGTTGCAACAGTGGGATAATCACACAAATCCCCAAGCTAGGGAGAAACAGGTCCAAGATTTGGCTTGTAATATTGAGGATGCTATTAACGAGTACCTGCTTCAGGTTTCACAGCATTCCCATACCAATCGGTTCTCTAAGCTGGCGCACAACGTTGATCATTTCATCCCCGACTGGATAGCAAGCAGTAAGTTATCCTCCAGCATTGATGAACTGGAACGAGACCTGAATTTGGTTACGTTACTAGATTCAGTACATTCAAATGGTCCCCAAGAAGGCCGCCCAAGCTCCAGCACAGGACCTCAGGGGGCAACTTTGACCCACAGATTTCTTGAAGACCATGAAATAGTGGGCTATGATGAAGAGAAAACACGACTTTTCCAACACTTGGGAGAAAGAAATCCAGAGCTTTTGACAATTGTGATTGTAGGTCCTGGAGGCTCCGGCAAAACTACACTCGTAAAGAATGTTTACCAAAGCGAAAGGGTCAGCAGCTTGTTTCATTTTCAAGCTTGGATTGATGTGCCACTCGATCTTGACCAACTCCTGCCGAAGTTGTTAAGAAGATTTAAGCAGGAAGTAGATCCTCATGAAGATATCAGTGACCTTCAAGAGAAACTGAGAAGAATTTTGGAGAATAAGAAGTTTTTGGTTGTCCTGGATAATGTTTGGAGCCACGAAGCTTTCGAATCCATTGTAAATGCACTACCAAAAACGGAGGGTAGTAAACTAATCGTAATTACTCGGAACATTGATGTAGCTCCGGTTTCCTCCAATTATATTCATGATTTGTGTGCTGGGTTATCATCGAAGAAAGCCAACCAGCTATTCTGCAAGAAGGCCTTTCCTGATGTTGTACGAAATATTTGTCCAACCCATCTGGAGGACTGGGCTGAACAGATTGTAACGAGGTGTGAAGGTATGCCCTGGGCAATTTCACAAATCGGCACTTTGCTAGCAAGGACGCCGAGAGTAGCAGTTGAGTGGAAGAAGATGCATGACACACTAGGATCCGATCTCTCCATTGCTAGCAGTATTTCACATTATAGGGATCTGCCAAGCAATCACAAAGAATGTTTCCTGTACTTCGGTATGTTTCCAGAAGACGAAACCATCAGCCGAGAGAGGCTAATACGGTTGTGGATAGCCGAAGGATTTGTGAGACCAGACGGAGTAAAAACGGTGGAGGCAGTTGCACAGACTATTCTGACTAATCTCATTGACAGAAATTTGGTTGTGGTCAGTGCGAGGGAAATCGATGGACGAGTCAAGAATTGTCGTGTTTCCAACCTTGTTCGTGAGTTCATCATTTCCAAAGCAAAGCATTTCATCACTGTTTTGGAAGCCAAGAGTGTTGGAAATATTGTTCGGGATTCAGGTGAGAAAATTCGACGCCTTTCAGTTCAGTGTGATATCAATTTGTTAAGGAACATAGACCTGAGTCGTATGACTCATACTCGTACCTTACTAGTTTTCCGGCAGACAGCCAATAGGTCTGAGCTTCATATGGTGCTTAAAGCCTGTAAGCTCTTACGGGTCTTCGGTTTGCAAGGTGTACCTGAATTGGTGAACTTTCCGGAATCTGTAATTAGTCTCACCTTATTAAGGTACTTGAGTGTCAGGCAAACCAACATTGAAAGTGTTCCAAGATCCATAGAGAAGCTTGGATTGTTGGAAACCTTAGAACTTAAAGACACCCTGGTGACCAATTTGCCAAAAGGGATTGGTAAACTCCACCATTTGCGCCACCTTTTGGTGTATTGCAATGCTGTCAACAATAATATGATGTTTGGAGCTGCACAAGGAGTGGAGTTTTCTACTAGCAAAATAAGTGCTTTATCTATTTCAAATTCGTTACAAAAGTTGTCTCTGATTAAGGTAAACACCAACAAAAAATTCTTGAAAGCCTTAGGAGAGTTGAAAGATCTTAGAAGGTTGGGGCTGGTAGATCTTCATAGAGAAGATGGAAGAGAATTGTGTTGCACCATTCAGAAAATGGATGATCTTTCGACATTGGATGTAAGAGCAACAAGCGAGGATGAATATCTTGACTTGGATCACATGCAATCCGGCTCCCCGCCTCTCCTTCTCCAACATCTATATTTAAAAGGGCGGCTAAAAAGGGTACCAGAGTGGATTCCCAAGCTTACCAGTCTCAAAAAGATTGGTTTAAAATGGTCAAAGATGGATGCTGACGCTGAGCCACTTACGGCCCTTCAAGATTTGCCCAACCTCATGGAGCTCGATTTGGTTGACTATTACACTGGCCAGGTGTTGGGTTTCGGAGATGAAACTTTCAAGAAACTAGTGGCATTAAGTATAGACAAATTTGATAAGCTAAATTTGATCTCAATAGGTGAGAAGGCAATGCCTATGCTCAAGAAGCTAACCATCTCGAGATGTCCGGATTTGAAGTTACTTCCATTTTCAAGTGGTGATCTTGAATTGCTGGAAGAATTGCTTCTATTTGACATGCCCAATGAATTCATTGCTGAGTTTGAAACAGACAGCGAACGACGTGAGGATTTAAAGCATGTTGGAGTCATTCATTATTCTCGGCATCCAGGAAGCTACCAATCTGATGGATTCAAAGATCTTTCTCCATCAAAATGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

913

Amino Acids

103.53

Weight (kDa)

6.69

Isoelectric Point (pI)

42.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 162 - 325 4e-26 NB-ARC domain
AAA_22 PF13401 181 - 264 4.9e-06 AAA domain
WHD_DRP PF23559 406 - 477 3.3e-17 Disease resistance protein Winged helix domain
LRR_14 PF23598 526 - 840 1.6e-35 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 228
Acc65I GGTACC 1 cut(s) 2248
AccB1I GGYRCC 1 cut(s) 2248
AccB7I CCANNNNNTGG 4 cut(s) 184, 511, 1876, 2398
AccIII TCCGGA 2 cut(s) 1699, 2527
AciI CCGC 4 cut(s) 21, 403, 2201, 2236
AclI AACGTT 1 cut(s) 276
AclWI GGATC 7 cut(s) 102, 701, 1128, 1141, 1182, 1767, 2187
AcoI YGGCCR 1 cut(s) 2389
AcsI RAATTY 9 cut(s) 352, 750, 1351, 1509, 1972, 2018, 2450, 2464, 2606
AcuI CTGAAG 1 cut(s) 209
AcyI GRCGYC 2 cut(s) 1088, 1516
AfaI GTAC 9 cut(s) 76, 218, 378, 981, 1208, 1585, 1680, 1733, 2250
AhlI ACTAGT 2 cut(s) 1591, 2428
AjiI CACGTC 1 cut(s) 2642
AjnI CCWGG 6 cut(s) 556, 780, 1049, 1821, 2391, 2686
AjuI GAANNNNNNNTTGG 4 cut(s) 935, 967, 2003, 2035
AleI CACNNNNGTG 1 cut(s) 1823
AloI GAACNNNNNNTCC 2 cut(s) 1473, 1505
Alw21I GWGCWC 1 cut(s) 2367
Alw26I GTCTC 6 cut(s) 32, 339, 1279, 1721, 1995, 2282
AlwI GGATC 7 cut(s) 102, 701, 1128, 1141, 1182, 1767, 2187
Ama87I CYCGRG 1 cut(s) 2518
Aor13HI TCCGGA 2 cut(s) 1699, 2527
AoxI GGCC 5 cut(s) 105, 400, 963, 2334, 2389
ApaI GGGCCC 1 cut(s) 109
ApeKI GCWGC 2 cut(s) 615, 1919
ApoI RAATTY 9 cut(s) 352, 750, 1351, 1509, 1972, 2018, 2450, 2464, 2606
ArsI GACNNNNNNTTYG 2 cut(s) 976, 1008
Asp700I GAANNNNTTC 2 cut(s) 1764, 2583
Asp718I GGTACC 1 cut(s) 2248
AspLEI GCGC 2 cut(s) 271, 1869
AspS9I GGNCC 7 cut(s) 105, 106, 175, 389, 422, 554, 2335
AsuC2I CCSGG 1 cut(s) 109
AsuHPI GGTGA 6 cut(s) 1514, 1702, 1711, 1837, 2492, 2570
AsuII TTCGAA 1 cut(s) 810
AsuNHI GCTAGC 2 cut(s) 1078, 1151
AvaI CYCGRG 1 cut(s) 2518
AvaII GGWCC 4 cut(s) 175, 389, 422, 554
AxyI CCTNAGG 2 cut(s) 426, 2032
BaeGI GKGCMC 1 cut(s) 109
BaeI ACNNNNGTAYC 4 cut(s) 1723, 1756, 2240, 2273
BalI TGGCCA 1 cut(s) 2391
BamHI GGATCC 1 cut(s) 1133
BanI GGYRCC 1 cut(s) 2248
BanII GRGCYC 2 cut(s) 109, 2367
BbsI GAAGAC 3 cut(s) 465, 1233, 1654
Bbv12I GWGCWC 1 cut(s) 2367
BbvI GCAGC 2 cut(s) 627, 1906
BccI CCATC 8 cut(s) 1008, 1244, 1376, 2081, 2302, 2522, 2699, 2734
BceAI ACGGC 2 cut(s) 57, 2349
BciT130I CCWGG 6 cut(s) 558, 782, 1051, 1823, 2393, 2688
BclI TGATCA 1 cut(s) 280
BcnI CCSGG 1 cut(s) 109
BcoDI GTCTC 6 cut(s) 32, 339, 1279, 1721, 1995, 2282
BcuI ACTAGT 2 cut(s) 1591, 2428
BfaI CTAG 8 cut(s) 162, 368, 1079, 1130, 1152, 1592, 1944, 2429
BfuAI ACCTGC 1 cut(s) 228
BglII AGATCT 3 cut(s) 2047, 2071, 2716
BisI GCNGC 5 cut(s) 21, 403, 616, 1920, 2237
BlpI GCTNAGC 1 cut(s) 2322
BlsI GCNGC 5 cut(s) 22, 404, 617, 1921, 2238
Bme1390I CCNGG 7 cut(s) 109, 558, 782, 1051, 1823, 2393, 2688
Bme18I GGWCC 4 cut(s) 175, 389, 422, 554
BmeT110I CYCGRG 1 cut(s) 2518
BmgBI CACGTC 1 cut(s) 2642
BmgT120I GGNCC 7 cut(s) 105, 106, 175, 389, 422, 554, 2335
BmiI GGNNCC 7 cut(s) 107, 391, 565, 797, 1135, 2197, 2250
BmrFI CCNGG 7 cut(s) 109, 558, 782, 1051, 1823, 2393, 2688
BmrI ACTGGG 1 cut(s) 1021
BmsI GCATC 4 cut(s) 193, 1107, 2302, 2692
BmtI GCTAGC 2 cut(s) 1082, 1155
BmuI ACTGGG 1 cut(s) 1021
BoxI GACNNNNGTC 1 cut(s) 1563
BpiI GAAGAC 3 cut(s) 465, 1233, 1654
BpmI CTGGAG 4 cut(s) 307, 397, 579, 1025
Bpu1102I GCTNAGC 1 cut(s) 2322
Bpu14I TTCGAA 1 cut(s) 810
BpuEI CTTGAG 2 cut(s) 1756, 2486
BpuMI CCSGG 1 cut(s) 109
Bsa29I ATCGAT 1 cut(s) 1380
BsaBI GATNNNNATC 1 cut(s) 2166
BsaHI GRCGYC 2 cut(s) 1088, 1516
BsaI GGTCTC 2 cut(s) 339, 1279
BsaJI CCNNGG 3 cut(s) 1049, 1050, 1821
BsaWI WCCGGW 3 cut(s) 886, 1699, 2527
Bse1I ACTGG 5 cut(s) 302, 342, 1016, 2274, 2392
Bse21I CCTNAGG 2 cut(s) 426, 2032
Bse3DI GCAATG 5 cut(s) 23, 1146, 1894, 2496, 2610
Bse8I GATNNNNATC 1 cut(s) 2166
BseAI TCCGGA 2 cut(s) 1699, 2527
BseBI CCWGG 6 cut(s) 558, 782, 1051, 1823, 2393, 2688
BseCI ATCGAT 1 cut(s) 1380
BseDI CCNNGG 3 cut(s) 1049, 1050, 1821
BseGI GGATG 7 cut(s) 208, 288, 2128, 2146, 2167, 2317, 2683
BseJI GATNNNNATC 1 cut(s) 2166
BseMI GCAATG 5 cut(s) 23, 1146, 1894, 2496, 2610
BseMII CTCAG 6 cut(s) 440, 734, 1553, 1611, 2313, 2607
BseNI ACTGG 5 cut(s) 302, 342, 1016, 2274, 2392
BseSI GKGCMC 1 cut(s) 109
BseXI GCAGC 2 cut(s) 627, 1906
BseYI CCCAGC 1 cut(s) 920
BsgI GTGCAG 2 cut(s) 37, 1905
BshFI GGCC 5 cut(s) 107, 402, 965, 2336, 2391
BshNI GGYRCC 1 cut(s) 2248
BshVI ATCGAT 1 cut(s) 1380
BsiHKAI GWGCWC 1 cut(s) 2367
BsiHKCI CYCGRG 1 cut(s) 2518
BsiSI CCGG 7 cut(s) 109, 567, 887, 1600, 1700, 2193, 2528
BsiWI CGTACG 1 cut(s) 74
BslFI GGGAC 1 cut(s) 375
BsmAI GTCTC 6 cut(s) 32, 339, 1279, 1721, 1995, 2282
BsmBI CGTCTC 1 cut(s) 32
BsmFI GGGAC 1 cut(s) 375
BsmI GAATGC 1 cut(s) 238
BsnI GGCC 5 cut(s) 107, 402, 965, 2336, 2391
Bso31I GGTCTC 2 cut(s) 339, 1279
BsoBI CYCGRG 1 cut(s) 2518
Bsp119I TTCGAA 1 cut(s) 810
Bsp120I GGGCCC 1 cut(s) 105
Bsp1286I GDGCHC 2 cut(s) 109, 2367
Bsp13I TCCGGA 2 cut(s) 1699, 2527
Bsp1720I GCTNAGC 1 cut(s) 2322
BspACI CCGC 4 cut(s) 21, 403, 2201, 2236
BspANI GGCC 5 cut(s) 107, 402, 965, 2336, 2391
BspCNI CTCAG 6 cut(s) 439, 735, 1554, 1612, 2314, 2608
BspDI ATCGAT 1 cut(s) 1380
BspEI TCCGGA 2 cut(s) 1699, 2527
BspHI TCATGA 2 cut(s) 712, 907
BspLI GGNNCC 7 cut(s) 107, 391, 565, 797, 1135, 2197, 2250
BspMI ACCTGC 1 cut(s) 228
BspOI GCTAGC 2 cut(s) 1082, 1155
BspPI GGATC 7 cut(s) 102, 701, 1128, 1141, 1182, 1767, 2187
BspT104I TTCGAA 1 cut(s) 810
BspT107I GGYRCC 1 cut(s) 2248
BspTNI GGTCTC 2 cut(s) 339, 1279
BsrDI GCAATG 5 cut(s) 23, 1146, 1894, 2496, 2610
BsrI ACTGG 5 cut(s) 302, 342, 1016, 2274, 2392
BssECI CCNNGG 3 cut(s) 1049, 1050, 1821
BssNI GRCGYC 2 cut(s) 1088, 1516
Bst2UI CCWGG 6 cut(s) 558, 782, 1051, 1823, 2393, 2688
Bst4CI ACNGT 5 cut(s) 71, 135, 1260, 1306, 1459
Bst6I CTCTTC 2 cut(s) 480, 2086
BstACI GRCGYC 2 cut(s) 1088, 1516
BstBI TTCGAA 1 cut(s) 810
BstC8I GCNNGC 4 cut(s) 267, 307, 1080, 1153
BstEII GGTNACC 1 cut(s) 1825
BstENI CCTNNNNNAGG 2 cut(s) 224, 1726
BstF5I GGATG 7 cut(s) 208, 288, 2128, 2146, 2167, 2317, 2683
BstHHI GCGC 2 cut(s) 271, 1869
BstMAI GTCTC 6 cut(s) 32, 339, 1279, 1721, 1995, 2282
BstMWI GCNNNNNNNGC 3 cut(s) 17, 408, 2506
BstNI CCWGG 6 cut(s) 558, 782, 1051, 1823, 2393, 2688
BstNSI RCATGY 3 cut(s) 2188, 2599, 2660
BstPAI GACNNNNGTC 1 cut(s) 1563
BstPI GGTNACC 1 cut(s) 1825
BstSCI CCNGG 7 cut(s) 107, 556, 780, 1049, 1821, 2391, 2686
BstSLI GKGCMC 1 cut(s) 109
BstV1I GCAGC 2 cut(s) 627, 1906
BstV2I GAAGAC 3 cut(s) 465, 1233, 1654
BstX2I RGATCY 8 cut(s) 94, 706, 1133, 1174, 1772, 2047, 2071, 2716
BstXI CCANNNNNNTGG 3 cut(s) 2359, 2705, 2733
BstYI RGATCY 8 cut(s) 94, 706, 1133, 1174, 1772, 2047, 2071, 2716
Bsu15I ATCGAT 1 cut(s) 1380
Bsu36I CCTNAGG 2 cut(s) 426, 2032
BsuRI GGCC 5 cut(s) 107, 402, 965, 2336, 2391
BsuTUI ATCGAT 1 cut(s) 1380
BtrI CACGTC 1 cut(s) 2642
BtsCI GGATG 7 cut(s) 208, 288, 2128, 2146, 2167, 2317, 2683
BtsIMutI CAGTG 6 cut(s) 140, 730, 1372, 1455, 1535, 2385
BveI ACCTGC 1 cut(s) 228
Cac8I GCNNGC 4 cut(s) 267, 307, 1080, 1153
CciI TCATGA 2 cut(s) 712, 907
CfoI GCGC 2 cut(s) 271, 1869
Cfr13I GGNCC 7 cut(s) 105, 106, 175, 389, 422, 554, 2335
ClaI ATCGAT 1 cut(s) 1380
CseI GACGC 3 cut(s) 1096, 1524, 2327
Csp6I GTAC 9 cut(s) 75, 217, 377, 980, 1207, 1584, 1679, 1732, 2249
CviAII CATG 8 cut(s) 464, 713, 908, 1121, 2185, 2359, 2596, 2657
CviQI GTAC 9 cut(s) 75, 217, 377, 980, 1207, 1584, 1679, 1732, 2249
DraI TTTAAA 3 cut(s) 2229, 2295, 2652
EaeI YGGCCR 1 cut(s) 2389
Eam1104I CTCTTC 2 cut(s) 480, 2086
EarI CTCTTC 2 cut(s) 480, 2086
Ecl136II GAGCTC 1 cut(s) 2365
Eco147I AGGCCT 1 cut(s) 965
Eco24I GRGCYC 2 cut(s) 109, 2367
Eco31I GGTCTC 2 cut(s) 339, 1279
Eco32I GATATC 2 cut(s) 721, 1537
Eco47I GGWCC 4 cut(s) 175, 389, 422, 554
Eco53kI GAGCTC 1 cut(s) 2365
Eco57I CTGAAG 1 cut(s) 209
Eco81I CCTNAGG 2 cut(s) 426, 2032
Eco88I CYCGRG 1 cut(s) 2518
Eco91I GGTNACC 1 cut(s) 1825
EcoICRI GAGCTC 1 cut(s) 2365
EcoNI CCTNNNNNAGG 2 cut(s) 224, 1726
EcoO109I RGGNCCY 3 cut(s) 105, 422, 554
EcoO65I GGTNACC 1 cut(s) 1825
EcoRI GAATTC 1 cut(s) 2606
EcoRII CCWGG 6 cut(s) 556, 780, 1049, 1821, 2391, 2686
EcoRV GATATC 2 cut(s) 721, 1537
EcoT22I ATGCAT 1 cut(s) 1122
EcoT38I GRGCYC 2 cut(s) 109, 2367
Esp3I CGTCTC 1 cut(s) 32
FaeI CATG 8 cut(s) 467, 716, 911, 1124, 2188, 2362, 2599, 2660
FalI AAGNNNNNCTT 4 cut(s) 950, 982, 2568, 2600
FaqI GGGAC 1 cut(s) 375
FatI CATG 8 cut(s) 463, 712, 907, 1120, 2184, 2358, 2595, 2656
FauI CCCGC 1 cut(s) 2208
FauNDI CATATG 1 cut(s) 1629
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 5 cut(s) 21, 403, 616, 1920, 2237
FokI GGATG 7 cut(s) 215, 275, 2135, 2153, 2174, 2324, 2670
FriOI GRGCYC 2 cut(s) 109, 2367
Fsp4HI GCNGC 5 cut(s) 21, 403, 616, 1920, 2237
FspBI CTAG 8 cut(s) 162, 368, 1079, 1130, 1152, 1592, 1944, 2429
GlaI GCGC 2 cut(s) 270, 1868
GluI GCNGC 5 cut(s) 21, 403, 616, 1920, 2237
GsaI CCCAGC 1 cut(s) 924
GsuI CTGGAG 4 cut(s) 307, 397, 579, 1025
HaeIII GGCC 5 cut(s) 107, 402, 965, 2336, 2391
HapII CCGG 7 cut(s) 109, 567, 887, 1600, 1700, 2193, 2528
HgaI GACGC 3 cut(s) 1096, 1524, 2327
HhaI GCGC 2 cut(s) 271, 1869
Hin1I GRCGYC 2 cut(s) 1088, 1516
Hin1II CATG 8 cut(s) 467, 716, 911, 1124, 2188, 2362, 2599, 2660
Hin6I GCGC 2 cut(s) 269, 1867
HinP1I GCGC 2 cut(s) 269, 1867
HincII GTYRAC 2 cut(s) 1897, 2377
HindII GTYRAC 2 cut(s) 1897, 2377
HindIII AAGCTT 4 cut(s) 633, 804, 1784, 2267
HpaII CCGG 7 cut(s) 109, 567, 887, 1600, 1700, 2193, 2528
HphI GGTGA 6 cut(s) 1514, 1702, 1711, 1837, 2492, 2570
Hpy166II GTNNAC 8 cut(s) 595, 851, 1679, 1693, 1853, 1897, 2005, 2377
Hpy188I TCNGA 8 cut(s) 870, 1138, 1332, 1621, 1995, 2115, 2408, 2704
Hpy8I GTNNAC 8 cut(s) 595, 851, 1679, 1693, 1853, 1897, 2005, 2377
Hpy99I CGWCG 2 cut(s) 1518, 2643
HpyAV CCTTC 9 cut(s) 392, 740, 955, 1034, 1268, 2049, 2219, 2348, 2479
HpyCH4III ACNGT 5 cut(s) 71, 135, 1260, 1306, 1459
HpyCH4IV ACGT 3 cut(s) 276, 362, 2641
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 408, 2506
HpySE526I ACGT 3 cut(s) 276, 362, 2641
Hsp92I GRCGYC 2 cut(s) 1088, 1516
Hsp92II CATG 8 cut(s) 467, 716, 911, 1124, 2188, 2362, 2599, 2660
HspAI GCGC 2 cut(s) 269, 1867
Kpn2I TCCGGA 2 cut(s) 1699, 2527
KpnI GGTACC 1 cut(s) 2252
Ksp22I TGATCA 1 cut(s) 280
LmnI GCTCC 7 cut(s) 416, 569, 795, 889, 1916, 2201, 2362
Lsp1109I GCAGC 2 cut(s) 627, 1906
LweI GCATC 4 cut(s) 193, 1107, 2302, 2692
MaeI CTAG 8 cut(s) 162, 368, 1079, 1130, 1152, 1592, 1944, 2429
MaeII ACGT 3 cut(s) 276, 362, 2641
MaeIII GTNAC 7 cut(s) 358, 363, 725, 1027, 1825, 1977, 2538
MfeI CAATTG 1 cut(s) 540
MflI RGATCY 8 cut(s) 94, 706, 1133, 1174, 1772, 2047, 2071, 2716
MhlI GDGCHC 2 cut(s) 109, 2367
MlsI TGGCCA 1 cut(s) 2391
MluNI TGGCCA 1 cut(s) 2391
MlyI GAGTC 4 cut(s) 1398, 1567, 1573, 2673
MmeI TCCRAC 7 cut(s) 529, 1019, 1434, 1459, 1776, 2239, 2641
Mox20I TGGCCA 1 cut(s) 2391
Mph1103I ATGCAT 1 cut(s) 1122
MroI TCCGGA 2 cut(s) 1699, 2527
MroXI GAANNNNTTC 2 cut(s) 1764, 2583
MscI TGGCCA 1 cut(s) 2391
MslI CAYNNNNRTG 3 cut(s) 384, 1823, 2731
Msp20I TGGCCA 1 cut(s) 2391
MspI CCGG 7 cut(s) 109, 567, 887, 1600, 1700, 2193, 2528
MspR9I CCNGG 7 cut(s) 109, 558, 782, 1051, 1823, 2393, 2688
MunI CAATTG 1 cut(s) 540
Mva1269I GAATGC 1 cut(s) 238
MvaI CCWGG 6 cut(s) 558, 782, 1051, 1823, 2393, 2688
MwoI GCNNNNNNNGC 3 cut(s) 17, 408, 2506
NciI CCSGG 1 cut(s) 109
NdeI CATATG 1 cut(s) 1629
NheI GCTAGC 2 cut(s) 1078, 1151
NlaIII CATG 8 cut(s) 467, 716, 911, 1124, 2188, 2362, 2599, 2660
NlaIV GGNNCC 7 cut(s) 107, 391, 565, 797, 1135, 2197, 2250
NmeAIII GCCGAG 3 cut(s) 1116, 1268, 2659
NmuCI GTSAC 2 cut(s) 725, 1825
NsiI ATGCAT 1 cut(s) 1122
NspI RCATGY 3 cut(s) 2188, 2599, 2660
NspV TTCGAA 1 cut(s) 810
OliI CACNNNNGTG 1 cut(s) 1823
PaeR7I CTCGAG 1 cut(s) 2518
PagI TCATGA 2 cut(s) 712, 907
PasI CCCWGGG 1 cut(s) 1050
PceI AGGCCT 1 cut(s) 965
PcsI WCGNNNNNNNCGW 1 cut(s) 807
PctI GAATGC 1 cut(s) 238
PdmI GAANNNNTTC 2 cut(s) 1764, 2583
PfeI GAWTC 6 cut(s) 371, 812, 1496, 1703, 2260, 2709
Pfl23II CGTACG 1 cut(s) 74
PflMI CCANNNNNTGG 4 cut(s) 184, 511, 1876, 2398
PfoI TCCNGGA 3 cut(s) 556, 780, 2686
PkrI GCNGC 5 cut(s) 22, 404, 617, 1921, 2238
PleI GAGTC 4 cut(s) 1397, 1567, 1572, 2672
PpsI GAGTC 4 cut(s) 1397, 1567, 1572, 2672
PpuMI RGGWCCY 2 cut(s) 422, 554
PshAI GACNNNNGTC 1 cut(s) 1563
Psp124BI GAGCTC 1 cut(s) 2367
Psp1406I AACGTT 1 cut(s) 276
Psp5II RGGWCCY 2 cut(s) 422, 554
Psp6I CCWGG 6 cut(s) 556, 780, 1049, 1821, 2391, 2686
PspEI GGTNACC 1 cut(s) 1825
PspFI CCCAGC 1 cut(s) 920
PspGI CCWGG 6 cut(s) 556, 780, 1049, 1821, 2391, 2686
PspLI CGTACG 1 cut(s) 74
PspN4I GGNNCC 7 cut(s) 107, 391, 565, 797, 1135, 2197, 2250
PspOMI GGGCCC 1 cut(s) 105
PspPI GGNCC 7 cut(s) 105, 106, 175, 389, 422, 554, 2335
PspPPI RGGWCCY 2 cut(s) 422, 554
PsuI RGATCY 8 cut(s) 94, 706, 1133, 1174, 1772, 2047, 2071, 2716
RsaI GTAC 9 cut(s) 76, 218, 378, 981, 1208, 1585, 1680, 1733, 2250
RsaNI GTAC 9 cut(s) 75, 217, 377, 980, 1207, 1584, 1679, 1732, 2249
RseI CAYNNNNRTG 3 cut(s) 384, 1823, 2731
SacI GAGCTC 1 cut(s) 2367
SatI GCNGC 5 cut(s) 21, 403, 616, 1920, 2237
Sau96I GGNCC 7 cut(s) 105, 106, 175, 389, 422, 554, 2335
SchI GAGTC 4 cut(s) 1398, 1567, 1573, 2673
ScrFI CCNGG 7 cut(s) 109, 558, 782, 1051, 1823, 2393, 2688
SduI GDGCHC 2 cut(s) 109, 2367
SfaNI GCATC 4 cut(s) 193, 1107, 2302, 2692
Sfr274I CTCGAG 1 cut(s) 2518
SfuI TTCGAA 1 cut(s) 810
SinI GGWCC 4 cut(s) 175, 389, 422, 554
SlaI CTCGAG 1 cut(s) 2518
SmiMI CAYNNNNRTG 3 cut(s) 384, 1823, 2731
SmlI CTYRAG 3 cut(s) 1735, 2501, 2518
SmoI CTYRAG 3 cut(s) 1735, 2501, 2518
SpeI ACTAGT 2 cut(s) 1591, 2428
SseBI AGGCCT 1 cut(s) 965
SsiI CCGC 4 cut(s) 21, 403, 2201, 2236
SspI AATATT 3 cut(s) 196, 988, 1486
SspMI CTAG 8 cut(s) 162, 368, 1079, 1130, 1152, 1592, 1944, 2429
SstI GAGCTC 1 cut(s) 2367
StuI AGGCCT 1 cut(s) 965
StyD4I CCNGG 7 cut(s) 107, 556, 780, 1049, 1821, 2391, 2686
TaaI ACNGT 5 cut(s) 71, 135, 1260, 1306, 1459
TaiI ACGT 3 cut(s) 279, 365, 2644
TaqI TCGA 8 cut(s) 654, 810, 932, 1380, 1513, 2132, 2367, 2519
TatI WGTACW 2 cut(s) 376, 1206
TauI GCSGC 3 cut(s) 23, 405, 2239
TfiI GAWTC 6 cut(s) 371, 812, 1496, 1703, 2260, 2709
TscAI CASTG 6 cut(s) 140, 730, 1372, 1462, 1535, 2392
TseFI GTSAC 2 cut(s) 725, 1825
TseI GCWGC 2 cut(s) 615, 1919
Tsp45I GTSAC 2 cut(s) 725, 1825
TspGWI ACGGA 3 cut(s) 77, 854, 1308
TspRI CASTG 6 cut(s) 140, 730, 1372, 1462, 1535, 2392
Van91I CCANNNNNTGG 4 cut(s) 184, 511, 1876, 2398
VpaK11BI GGWCC 4 cut(s) 175, 389, 422, 554
XagI CCTNNNNNAGG 2 cut(s) 224, 1726
XapI RAATTY 9 cut(s) 352, 750, 1351, 1509, 1972, 2018, 2450, 2464, 2606
XceI RCATGY 3 cut(s) 2188, 2599, 2660
XcmI CCANNNNNNNNNTGG 1 cut(s) 2116
XhoI CTCGAG 1 cut(s) 2518
XmnI GAANNNNTTC 2 cut(s) 1764, 2583
XspI CTAG 8 cut(s) 162, 368, 1079, 1130, 1152, 1592, 1944, 2429
Zsp2I ATGCAT 1 cut(s) 1122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.