Rh2BG614100

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000865
Physical Location & Seq
Reverse (-)
83707488 .. 83711593
4106 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG614100.1

Sequence Viewer

Length: 1499 bp
ATGTTTCCAGAAGACGAAACCATCAGCCGAGAGAGGCTAATACGGTTGTGGATAGCCGAAGGATTTGTGAGACCAGACGGAGTAAAAACGGTGGAGGCAGTTGCACAGACTATTCTGACTAATCTCATTGACAGAAATTTGGTTGTGGTCAGTGCGAGGGAAATCGATGGACGAGTCAAGAATTGTCATGTTTCCAACCTTGTTCGTGAGTTCATCATTTCCAAAGCAAAGCATTTCATCACTGTTTTGGAAGCCAAGAGTGTTGGAAATATTGTTCGGGATTCAGGTGAGAAAATTCGACGCCTTTCAGTTCAGTGTGATATCAATTTGTTAAGGAACATAGACCTGAGTCGTATGACTCATACTCGTACCTTACTAGTTTTCCGGCAGACAGCCAATAGGTCTGAGCTTCATATGGTGCTTAAAGCCTGTAAGCTCTTACGGGTCTTCGGTTTGCAAGGTGTACCTGAATTGGTGAACTTTCCAGAATCTGTAATTAGTCTCACCTTATTAAGGTTATTACAGTTTCCAAGATCCATAGAGAAGCTTGGATTGTTGGAAACCTTAGAACTTAATGACACCCTGGTGACCAATTTGCCAAAAGGGATTGGTAAACTCCACCATTTGCGCCACCTTTTGGTGTATTGCAATGCTGTCAACAATAATATGATGTTTGGAGCTGCACAAGGAGTGGAGTTTTCTACTAGCAAAAAAAGTGCTTTATCTATTTCAAATTCGTTACAAAAGTTGTCTCTGATTAAGGTAAACACCAACAAAAAATTCTTGAAAGCCTTAGGAGAGTTGAAAGATCTTAGAAGGTTGGGGCTGGTAGATCTTCATAGAGAAGATGGAAGAGAATTGTGTTGCACCATTCAGAAAATGGATGATCTTTCGACATTGGATGTAAGAGCAACAAGCGAGGATGAATATCTTGACTTGGATCACATGCAATCCGGCTCCCCGCCTCTCCTTCTCCAACATCTATATTTAAAAGGGCGGCTAAAAAGGGTAACAGAGTGGATTCCCAAGCTTACCAGTCTCAAAAAGATTGGTTTAAAATGGTCAAAGATGGATGCTGACGCTGAGCCACTTACGGCCCTTCAAGATTTGCCCAACCTCATGGAGCTCGATTTGGTTGACTATTACACTGGCCAGGTGTTGGGTTTCGGAGATGAAACTTTCAAGAAACTAGTGGCATTAAGTATAGACAAATTTGATAAGCTAAATTTGATCTCAATAGGTGAGAAGGCAATGCCTATGCTCAAGAAGCTAACCATCTCGAGATGTCAGAATTTGAAGTTACTTCCATTTTCAAGTGGTGATCTTGAATTGCTGGAAGAATTGCTTCTATTTGACATGCCCAATGAATTCATTGCTGAGTCTGAAACAGACAGCGAACGACGTGAGGATTTAAAGCATGTTGGAGTCATTCATTATTCTCGGCATCCAGGAAGCTACCAATCTGATGGATTCAAAGATCTTTCTCCATCAAAATGGAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

499

Amino Acids

56.65

Weight (kDa)

8.5

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 1 - 72 2.6e-17 Disease resistance protein Winged helix domain
LRR_14 PF23598 121 - 443 2.5e-25 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 637, 1159
AciI CCGC 2 cut(s) 962, 997
AclWI GGATC 2 cut(s) 528, 948
AcoI YGGCCR 1 cut(s) 1150
AcsI RAATTY 8 cut(s) 136, 294, 733, 779, 1211, 1225, 1291, 1367
AcyI GRCGYC 1 cut(s) 301
AfaI GTAC 2 cut(s) 370, 465
AfiI CCNNNNNNNGG 3 cut(s) 513, 637, 1159
AgsI TTSAA 9 cut(s) 732, 787, 805, 1103, 1183, 1297, 1314, 1328, 1474
AhlI ACTAGT 2 cut(s) 376, 1189
AjiI CACGTC 1 cut(s) 1403
AjnI CCWGG 3 cut(s) 582, 1152, 1447
AjuI GAANNNNNNNTTGG 2 cut(s) 764, 796
AleI CACNNNNGTG 1 cut(s) 584
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
AluBI AGCT 9 cut(s) 409, 436, 547, 680, 1030, 1126, 1222, 1270, 1455
AluI AGCT 9 cut(s) 409, 436, 547, 680, 1030, 1126, 1222, 1270, 1455
Alw21I GWGCWC 1 cut(s) 1128
Alw26I GTCTC 4 cut(s) 64, 506, 756, 1043
AlwI GGATC 2 cut(s) 528, 948
AlwNI CAGNNNCTG 1 cut(s) 491
Ama87I CYCGRG 1 cut(s) 1279
AoxI GGCC 2 cut(s) 1095, 1150
ApeKI GCWGC 1 cut(s) 680
ApoI RAATTY 8 cut(s) 136, 294, 733, 779, 1211, 1225, 1291, 1367
Asp700I GAANNNNTTC 1 cut(s) 1344
AspLEI GCGC 1 cut(s) 630
AspS9I GGNCC 1 cut(s) 1096
AsuHPI GGTGA 6 cut(s) 299, 487, 496, 598, 1253, 1331
AvaI CYCGRG 1 cut(s) 1279
AxyI CCTNAGG 1 cut(s) 793
BalI TGGCCA 1 cut(s) 1152
BanII GRGCYC 1 cut(s) 1128
BbsI GAAGAC 2 cut(s) 18, 439
Bbv12I GWGCWC 1 cut(s) 1128
BbvI GCAGC 1 cut(s) 667
BccI CCATC 7 cut(s) 29, 161, 842, 1063, 1283, 1460, 1495
BceAI ACGGC 1 cut(s) 1110
BciT130I CCWGG 3 cut(s) 584, 1154, 1449
BcoDI GTCTC 4 cut(s) 64, 506, 756, 1043
BcuI ACTAGT 2 cut(s) 376, 1189
BfaI CTAG 3 cut(s) 377, 705, 1190
BglII AGATCT 3 cut(s) 808, 832, 1477
BisI GCNGC 2 cut(s) 681, 998
BlpI GCTNAGC 1 cut(s) 1083
BlsI GCNGC 2 cut(s) 682, 999
Bme1390I CCNGG 3 cut(s) 584, 1154, 1449
BmeT110I CYCGRG 1 cut(s) 1279
BmgBI CACGTC 1 cut(s) 1403
BmgT120I GGNCC 1 cut(s) 1096
BmiI GGNNCC 1 cut(s) 958
BmrFI CCNGG 3 cut(s) 584, 1154, 1449
BmsI GCATC 2 cut(s) 1063, 1453
BoxI GACNNNNGTC 1 cut(s) 348
BpiI GAAGAC 2 cut(s) 18, 439
Bpu1102I GCTNAGC 1 cut(s) 1083
BpuEI CTTGAG 1 cut(s) 1247
Bsa29I ATCGAT 1 cut(s) 165
BsaBI GATNNNNATC 1 cut(s) 927
BsaHI GRCGYC 1 cut(s) 301
BsaI GGTCTC 1 cut(s) 64
BsaJI CCNNGG 1 cut(s) 582
Bsc4I CCNNNNNNNGG 3 cut(s) 513, 637, 1159
Bse1I ACTGG 2 cut(s) 1035, 1153
Bse21I CCTNAGG 1 cut(s) 793
Bse3DI GCAATG 3 cut(s) 655, 1257, 1371
Bse8I GATNNNNATC 1 cut(s) 927
BseBI CCWGG 3 cut(s) 584, 1154, 1449
BseCI ATCGAT 1 cut(s) 165
BseDI CCNNGG 1 cut(s) 582
BseGI GGATG 5 cut(s) 889, 907, 928, 1078, 1444
BseJI GATNNNNATC 1 cut(s) 927
BseLI CCNNNNNNNGG 3 cut(s) 513, 637, 1159
BseMI GCAATG 3 cut(s) 655, 1257, 1371
BseMII CTCAG 4 cut(s) 338, 396, 1074, 1368
BseNI ACTGG 2 cut(s) 1035, 1153
BseXI GCAGC 1 cut(s) 667
BsgI GTGCAG 1 cut(s) 666
BshFI GGCC 2 cut(s) 1097, 1152
BshVI ATCGAT 1 cut(s) 165
BsiHKAI GWGCWC 1 cut(s) 1128
BsiHKCI CYCGRG 1 cut(s) 1279
BsiSI CCGG 2 cut(s) 385, 954
BslI CCNNNNNNNGG 3 cut(s) 513, 637, 1159
BsmAI GTCTC 4 cut(s) 64, 506, 756, 1043
BsnI GGCC 2 cut(s) 1097, 1152
Bso31I GGTCTC 1 cut(s) 64
BsoBI CYCGRG 1 cut(s) 1279
Bsp1286I GDGCHC 1 cut(s) 1128
Bsp143I GATC 8 cut(s) 533, 808, 832, 886, 940, 1230, 1321, 1477
Bsp1720I GCTNAGC 1 cut(s) 1083
BspACI CCGC 2 cut(s) 962, 997
BspANI GGCC 2 cut(s) 1097, 1152
BspCNI CTCAG 4 cut(s) 339, 397, 1075, 1369
BspDI ATCGAT 1 cut(s) 165
BspLI GGNNCC 1 cut(s) 958
BspPI GGATC 2 cut(s) 528, 948
BspTNI GGTCTC 1 cut(s) 64
BsrDI GCAATG 3 cut(s) 655, 1257, 1371
BsrI ACTGG 2 cut(s) 1035, 1153
BssECI CCNNGG 1 cut(s) 582
BssMI GATC 8 cut(s) 533, 808, 832, 886, 940, 1230, 1321, 1477
BssNI GRCGYC 1 cut(s) 301
Bst2UI CCWGG 3 cut(s) 584, 1154, 1449
Bst4CI ACNGT 4 cut(s) 45, 91, 244, 525
Bst6I CTCTTC 1 cut(s) 847
BstACI GRCGYC 1 cut(s) 301
BstDEI CTNAG 7 cut(s) 347, 405, 565, 793, 812, 1083, 1377
BstEII GGTNACC 1 cut(s) 586
BstENI CCTNNNNNAGG 1 cut(s) 511
BstF5I GGATG 5 cut(s) 889, 907, 928, 1078, 1444
BstHHI GCGC 1 cut(s) 630
BstKTI GATC 8 cut(s) 536, 811, 835, 889, 943, 1233, 1324, 1480
BstMAI GTCTC 4 cut(s) 64, 506, 756, 1043
BstMBI GATC 8 cut(s) 533, 808, 832, 886, 940, 1230, 1321, 1477
BstMWI GCNNNNNNNGC 1 cut(s) 1267
BstNI CCWGG 3 cut(s) 584, 1154, 1449
BstNSI RCATGY 3 cut(s) 949, 1360, 1421
BstPAI GACNNNNGTC 1 cut(s) 348
BstPI GGTNACC 1 cut(s) 586
BstSCI CCNGG 3 cut(s) 582, 1152, 1447
BstV1I GCAGC 1 cut(s) 667
BstV2I GAAGAC 2 cut(s) 18, 439
BstX2I RGATCY 4 cut(s) 533, 808, 832, 1477
BstXI CCANNNNNNTGG 3 cut(s) 1120, 1466, 1494
BstYI RGATCY 4 cut(s) 533, 808, 832, 1477
Bsu15I ATCGAT 1 cut(s) 165
Bsu36I CCTNAGG 1 cut(s) 793
BsuRI GGCC 2 cut(s) 1097, 1152
BsuTUI ATCGAT 1 cut(s) 165
BtrI CACGTC 1 cut(s) 1403
BtsCI GGATG 5 cut(s) 889, 907, 928, 1078, 1444
BtsIMutI CAGTG 4 cut(s) 157, 240, 320, 1146
CaiI CAGNNNCTG 1 cut(s) 491
CfoI GCGC 1 cut(s) 630
Cfr13I GGNCC 1 cut(s) 1096
ClaI ATCGAT 1 cut(s) 165
CseI GACGC 2 cut(s) 309, 1088
Csp6I GTAC 2 cut(s) 369, 464
CviAII CATG 5 cut(s) 188, 946, 1120, 1357, 1418
CviQI GTAC 2 cut(s) 369, 464
DdeI CTNAG 7 cut(s) 347, 405, 565, 793, 812, 1083, 1377
DpnI GATC 8 cut(s) 535, 810, 834, 888, 942, 1232, 1323, 1479
DpnII GATC 8 cut(s) 533, 808, 832, 886, 940, 1230, 1321, 1477
DraI TTTAAA 3 cut(s) 990, 1056, 1413
EaeI YGGCCR 1 cut(s) 1150
Eam1104I CTCTTC 1 cut(s) 847
EarI CTCTTC 1 cut(s) 847
Ecl136II GAGCTC 1 cut(s) 1126
Eco24I GRGCYC 1 cut(s) 1128
Eco31I GGTCTC 1 cut(s) 64
Eco32I GATATC 1 cut(s) 322
Eco53kI GAGCTC 1 cut(s) 1126
Eco81I CCTNAGG 1 cut(s) 793
Eco88I CYCGRG 1 cut(s) 1279
Eco91I GGTNACC 1 cut(s) 586
EcoICRI GAGCTC 1 cut(s) 1126
EcoNI CCTNNNNNAGG 1 cut(s) 511
EcoO65I GGTNACC 1 cut(s) 586
EcoRI GAATTC 1 cut(s) 1367
EcoRII CCWGG 3 cut(s) 582, 1152, 1447
EcoRV GATATC 1 cut(s) 322
EcoT38I GRGCYC 1 cut(s) 1128
FaeI CATG 5 cut(s) 191, 949, 1123, 1360, 1421
FalI AAGNNNNNCTT 2 cut(s) 1329, 1361
FatI CATG 5 cut(s) 187, 945, 1119, 1356, 1417
FauI CCCGC 1 cut(s) 969
FauNDI CATATG 1 cut(s) 414
Fnu4HI GCNGC 2 cut(s) 681, 998
FokI GGATG 5 cut(s) 896, 914, 935, 1085, 1431
FriOI GRGCYC 1 cut(s) 1128
Fsp4HI GCNGC 2 cut(s) 681, 998
FspBI CTAG 3 cut(s) 377, 705, 1190
GlaI GCGC 1 cut(s) 629
GluI GCNGC 2 cut(s) 681, 998
HaeIII GGCC 2 cut(s) 1097, 1152
HapII CCGG 2 cut(s) 385, 954
HgaI GACGC 2 cut(s) 309, 1088
HhaI GCGC 1 cut(s) 630
Hin1I GRCGYC 1 cut(s) 301
Hin1II CATG 5 cut(s) 191, 949, 1123, 1360, 1421
Hin6I GCGC 1 cut(s) 628
HinP1I GCGC 1 cut(s) 628
HincII GTYRAC 2 cut(s) 658, 1138
HindII GTYRAC 2 cut(s) 658, 1138
HindIII AAGCTT 2 cut(s) 545, 1028
HinfI GANTC 9 cut(s) 174, 281, 349, 358, 488, 1021, 1379, 1425, 1470
HpaII CCGG 2 cut(s) 385, 954
HphI GGTGA 6 cut(s) 299, 487, 496, 598, 1253, 1331
Hpy166II GTNNAC 6 cut(s) 464, 478, 614, 658, 766, 1138
Hpy188I TCNGA 8 cut(s) 117, 406, 756, 876, 1169, 1290, 1384, 1465
Hpy8I GTNNAC 6 cut(s) 464, 478, 614, 658, 766, 1138
Hpy99I CGWCG 2 cut(s) 303, 1404
HpyAV CCTTC 5 cut(s) 53, 810, 980, 1109, 1240
HpyCH4III ACNGT 4 cut(s) 45, 91, 244, 525
HpyCH4IV ACGT 1 cut(s) 1402
HpyCH4V TGCA 6 cut(s) 104, 457, 648, 683, 867, 949
HpyF10VI GCNNNNNNNGC 1 cut(s) 1267
HpyF3I CTNAG 7 cut(s) 347, 405, 565, 793, 812, 1083, 1377
HpySE526I ACGT 1 cut(s) 1402
Hsp92I GRCGYC 1 cut(s) 301
Hsp92II CATG 5 cut(s) 191, 949, 1123, 1360, 1421
HspAI GCGC 1 cut(s) 628
Kzo9I GATC 8 cut(s) 533, 808, 832, 886, 940, 1230, 1321, 1477
LmnI GCTCC 3 cut(s) 677, 962, 1123
Lsp1109I GCAGC 1 cut(s) 667
LweI GCATC 2 cut(s) 1063, 1453
MaeI CTAG 3 cut(s) 377, 705, 1190
MaeII ACGT 1 cut(s) 1402
MaeIII GTNAC 4 cut(s) 586, 738, 1009, 1299
MalI GATC 8 cut(s) 535, 810, 834, 888, 942, 1232, 1323, 1479
MboI GATC 8 cut(s) 533, 808, 832, 886, 940, 1230, 1321, 1477
MboII GAAGA 6 cut(s) 23, 439, 827, 857, 864, 1349
MflI RGATCY 4 cut(s) 533, 808, 832, 1477
MhlI GDGCHC 1 cut(s) 1128
MlsI TGGCCA 1 cut(s) 1152
MluNI TGGCCA 1 cut(s) 1152
MlyI GAGTC 5 cut(s) 183, 352, 358, 1388, 1434
MmeI TCCRAC 5 cut(s) 219, 244, 537, 1000, 1402
MnlI CCTC 7 cut(s) 27, 88, 150, 913, 975, 1127, 1399
Mox20I TGGCCA 1 cut(s) 1152
MroXI GAANNNNTTC 1 cut(s) 1344
MscI TGGCCA 1 cut(s) 1152
MseI TTAA 9 cut(s) 332, 423, 512, 573, 759, 989, 1055, 1199, 1412
MslI CAYNNNNRTG 2 cut(s) 584, 1492
Msp20I TGGCCA 1 cut(s) 1152
MspI CCGG 2 cut(s) 385, 954
MspR9I CCNGG 3 cut(s) 584, 1154, 1449
MvaI CCWGG 3 cut(s) 584, 1154, 1449
MwoI GCNNNNNNNGC 1 cut(s) 1267
NdeI CATATG 1 cut(s) 414
NdeII GATC 8 cut(s) 533, 808, 832, 886, 940, 1230, 1321, 1477
NlaIII CATG 5 cut(s) 191, 949, 1123, 1360, 1421
NlaIV GGNNCC 1 cut(s) 958
NmeAIII GCCGAG 2 cut(s) 53, 1420
NmuCI GTSAC 1 cut(s) 586
NspI RCATGY 3 cut(s) 949, 1360, 1421
OliI CACNNNNGTG 1 cut(s) 584
PaeR7I CTCGAG 1 cut(s) 1279
PdmI GAANNNNTTC 1 cut(s) 1344
PfeI GAWTC 4 cut(s) 281, 488, 1021, 1470
PflMI CCANNNNNTGG 2 cut(s) 637, 1159
PfoI TCCNGGA 1 cut(s) 1447
PkrI GCNGC 2 cut(s) 682, 999
PleI GAGTC 5 cut(s) 182, 352, 357, 1387, 1433
PpsI GAGTC 5 cut(s) 182, 352, 357, 1387, 1433
PshAI GACNNNNGTC 1 cut(s) 348
Psp124BI GAGCTC 1 cut(s) 1128
Psp6I CCWGG 3 cut(s) 582, 1152, 1447
PspEI GGTNACC 1 cut(s) 586
PspGI CCWGG 3 cut(s) 582, 1152, 1447
PspN4I GGNNCC 1 cut(s) 958
PspPI GGNCC 1 cut(s) 1096
PstNI CAGNNNCTG 1 cut(s) 491
PsuI RGATCY 4 cut(s) 533, 808, 832, 1477
RsaI GTAC 2 cut(s) 370, 465
RsaNI GTAC 2 cut(s) 369, 464
RseI CAYNNNNRTG 2 cut(s) 584, 1492
SacI GAGCTC 1 cut(s) 1128
SaqAI TTAA 9 cut(s) 332, 423, 512, 573, 759, 989, 1055, 1199, 1412
SatI GCNGC 2 cut(s) 681, 998
Sau3AI GATC 8 cut(s) 533, 808, 832, 886, 940, 1230, 1321, 1477
Sau96I GGNCC 1 cut(s) 1096
SchI GAGTC 5 cut(s) 183, 352, 358, 1388, 1434
ScrFI CCNGG 3 cut(s) 584, 1154, 1449
SduI GDGCHC 1 cut(s) 1128
SfaNI GCATC 2 cut(s) 1063, 1453
Sfr274I CTCGAG 1 cut(s) 1279
SlaI CTCGAG 1 cut(s) 1279
SmiMI CAYNNNNRTG 2 cut(s) 584, 1492
SmlI CTYRAG 2 cut(s) 1262, 1279
SmoI CTYRAG 2 cut(s) 1262, 1279
SpeI ACTAGT 2 cut(s) 376, 1189
SsiI CCGC 2 cut(s) 962, 997
SspI AATATT 1 cut(s) 271
SspMI CTAG 3 cut(s) 377, 705, 1190
SstI GAGCTC 1 cut(s) 1128
StyD4I CCNGG 3 cut(s) 582, 1152, 1447
TaaI ACNGT 4 cut(s) 45, 91, 244, 525
TaiI ACGT 1 cut(s) 1405
TaqI TCGA 5 cut(s) 165, 298, 893, 1128, 1280
TauI GCSGC 1 cut(s) 1000
TfiI GAWTC 4 cut(s) 281, 488, 1021, 1470
Tru1I TTAA 9 cut(s) 332, 423, 512, 573, 759, 989, 1055, 1199, 1412
Tru9I TTAA 9 cut(s) 332, 423, 512, 573, 759, 989, 1055, 1199, 1412
TscAI CASTG 4 cut(s) 157, 247, 320, 1153
TseFI GTSAC 1 cut(s) 586
TseI GCWGC 1 cut(s) 680
Tsp45I GTSAC 1 cut(s) 586
TspDTI ATGAA 9 cut(s) 202, 226, 401, 827, 939, 1188, 1360, 1380, 1421
TspGWI ACGGA 1 cut(s) 93
TspRI CASTG 4 cut(s) 157, 247, 320, 1153
Van91I CCANNNNNTGG 2 cut(s) 637, 1159
XagI CCTNNNNNAGG 1 cut(s) 511
XapI RAATTY 8 cut(s) 136, 294, 733, 779, 1211, 1225, 1291, 1367
XceI RCATGY 3 cut(s) 949, 1360, 1421
XcmI CCANNNNNNNNNTGG 1 cut(s) 877
XhoI CTCGAG 1 cut(s) 1279
XmnI GAANNNNTTC 1 cut(s) 1344
XspI CTAG 3 cut(s) 377, 705, 1190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.