RchiOBHm_Chr2g0168221

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000865
Physical Location & Seq
Reverse (-)
82659705 .. 82660946
1242 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53590

Sequence Viewer

Length: 1041 bp
ATGCTCCCCTTTCTTTTATCAACAGTAAACCCACTAACAAGCTGCCAAAACAGGTTATTACAGTTTCCAAGATCCATAGAGAAGCTTGGATTGTTGGAAACCTTAGAACTTAATGACACCCTGGTGACCAATTTGCCAAAAGGGATTGGTAAACTCCACCATTTGCGCCACCTTTTGGTGTATTGCAATGCTGTCAACAATAATATGATGTTTGGAGCTGCACAAGGAGTGGAGTTTTCTACTAGCAAAAAAAGTGCTTTATCTATTTCAAATTCGTTACAAAAGTTGTCTCTGATTAAGGTAAACACCAACAAAAAATTCTTGAAAGCCTTAGGAGAGTTGAAAGATCTTAGAAGGTTGGGGCTGGTAGATCTTCATAGAGAAGATGGAAGAGAATTGTGTTGCACCATTCAGAAAATGGATGATCTTTCGACATTGGATGTAAGAGCAACAAGCGAGGATGAATATCTTGACTTGGATCACATGCAATCCGGCTCCCCGCCTCTCCTTCTCCAACATCTATATTTAAAAGGGCGGCTAAAAAGGGTAACAGAGTGGATTCCCAAGCTTACCAGTCTCAAAAAGATTGGTTTAAAATGGTCAAAGATGGATGCTGACGCTGAGCCACTTACGGCCCTTCAAGATTTGCCCAACCTCATGGAGCTCGATTTGGTTGACTATTACACTGGCCAGGTGTTGGGTTTCGGAGATGAAACTTTCAAGAAACTAGTGGCATTAAGTATAGACAAATTTGATAAGCTAAATTTGATCTCAATAGGTGAGAAGGCAATGCCTATGCTCAAGAAGCTAACCATCTCGAGATGTCAGAATTTGAAGTTACTTCCATTTTCAAGTGGTGATCTTGAATTGCTGGAAGAATTGCTTCTATTTGACATGCCCAATGAATTCATTGCTGAGTCTGAAACAGACAGCGAACGACGTGAGGATTTAAAGCATGTTGGAGTCATTCATTATTCTCGGCATCCAGGAAGCTACCAATCTGATGGATTCAAAGATCTTTCTCCATCAAAATGGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

346

Amino Acids

39.24

Weight (kDa)

6.77

Isoelectric Point (pI)

37.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 18 - 292 2.9e-25 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 175, 697
AciI CCGC 2 cut(s) 500, 535
AclWI GGATC 2 cut(s) 66, 486
AcoI YGGCCR 1 cut(s) 688
AcsI RAATTY 6 cut(s) 271, 317, 749, 763, 829, 905
AfiI CCNNNNNNNGG 2 cut(s) 175, 697
AgsI TTSAA 9 cut(s) 270, 325, 343, 641, 721, 835, 852, 866, 1012
AhlI ACTAGT 1 cut(s) 727
AjiI CACGTC 1 cut(s) 941
AjnI CCWGG 3 cut(s) 120, 690, 985
AjuI GAANNNNNNNTTGG 2 cut(s) 302, 334
AleI CACNNNNGTG 1 cut(s) 122
AluBI AGCT 8 cut(s) 42, 85, 218, 568, 664, 760, 808, 993
AluI AGCT 8 cut(s) 42, 85, 218, 568, 664, 760, 808, 993
Alw21I GWGCWC 1 cut(s) 666
Alw26I GTCTC 2 cut(s) 294, 581
AlwI GGATC 2 cut(s) 66, 486
Ama87I CYCGRG 1 cut(s) 817
AoxI GGCC 2 cut(s) 633, 688
ApeKI GCWGC 2 cut(s) 42, 218
ApoI RAATTY 6 cut(s) 271, 317, 749, 763, 829, 905
Asp700I GAANNNNTTC 1 cut(s) 882
AspLEI GCGC 1 cut(s) 168
AspS9I GGNCC 1 cut(s) 634
AsuHPI GGTGA 3 cut(s) 136, 791, 869
AvaI CYCGRG 1 cut(s) 817
AxyI CCTNAGG 1 cut(s) 331
BalI TGGCCA 1 cut(s) 690
BanII GRGCYC 1 cut(s) 666
Bbv12I GWGCWC 1 cut(s) 666
BbvI GCAGC 2 cut(s) 29, 205
BccI CCATC 5 cut(s) 380, 601, 821, 998, 1033
BceAI ACGGC 1 cut(s) 648
BciT130I CCWGG 3 cut(s) 122, 692, 987
BcoDI GTCTC 2 cut(s) 294, 581
BcuI ACTAGT 1 cut(s) 727
BfaI CTAG 2 cut(s) 243, 728
BglII AGATCT 3 cut(s) 346, 370, 1015
BisI GCNGC 3 cut(s) 43, 219, 536
BlpI GCTNAGC 1 cut(s) 621
BlsI GCNGC 3 cut(s) 44, 220, 537
Bme1390I CCNGG 3 cut(s) 122, 692, 987
BmeT110I CYCGRG 1 cut(s) 817
BmgBI CACGTC 1 cut(s) 941
BmgT120I GGNCC 1 cut(s) 634
BmiI GGNNCC 1 cut(s) 496
BmrFI CCNGG 3 cut(s) 122, 692, 987
BmsI GCATC 2 cut(s) 601, 991
Bpu1102I GCTNAGC 1 cut(s) 621
BpuEI CTTGAG 1 cut(s) 785
BsaBI GATNNNNATC 1 cut(s) 465
BsaJI CCNNGG 1 cut(s) 120
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 697
Bse1I ACTGG 2 cut(s) 573, 691
Bse21I CCTNAGG 1 cut(s) 331
Bse3DI GCAATG 3 cut(s) 193, 795, 909
Bse8I GATNNNNATC 1 cut(s) 465
BseBI CCWGG 3 cut(s) 122, 692, 987
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 5 cut(s) 427, 445, 466, 616, 982
BseJI GATNNNNATC 1 cut(s) 465
BseLI CCNNNNNNNGG 2 cut(s) 175, 697
BseMI GCAATG 3 cut(s) 193, 795, 909
BseMII CTCAG 2 cut(s) 612, 906
BseNI ACTGG 2 cut(s) 573, 691
BseXI GCAGC 2 cut(s) 29, 205
BsgI GTGCAG 1 cut(s) 204
BshFI GGCC 2 cut(s) 635, 690
BsiHKAI GWGCWC 1 cut(s) 666
BsiHKCI CYCGRG 1 cut(s) 817
BsiSI CCGG 1 cut(s) 492
BslI CCNNNNNNNGG 2 cut(s) 175, 697
BsmAI GTCTC 2 cut(s) 294, 581
BsnI GGCC 2 cut(s) 635, 690
BsoBI CYCGRG 1 cut(s) 817
Bsp1286I GDGCHC 1 cut(s) 666
Bsp143I GATC 8 cut(s) 71, 346, 370, 424, 478, 768, 859, 1015
Bsp1720I GCTNAGC 1 cut(s) 621
BspACI CCGC 2 cut(s) 500, 535
BspANI GGCC 2 cut(s) 635, 690
BspCNI CTCAG 2 cut(s) 613, 907
BspLI GGNNCC 1 cut(s) 496
BspPI GGATC 2 cut(s) 66, 486
BsrDI GCAATG 3 cut(s) 193, 795, 909
BsrI ACTGG 2 cut(s) 573, 691
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 8 cut(s) 71, 346, 370, 424, 478, 768, 859, 1015
Bst2UI CCWGG 3 cut(s) 122, 692, 987
Bst4CI ACNGT 2 cut(s) 25, 63
Bst6I CTCTTC 1 cut(s) 385
BstDEI CTNAG 5 cut(s) 103, 331, 350, 621, 915
BstEII GGTNACC 1 cut(s) 124
BstF5I GGATG 5 cut(s) 427, 445, 466, 616, 982
BstHHI GCGC 1 cut(s) 168
BstKTI GATC 8 cut(s) 74, 349, 373, 427, 481, 771, 862, 1018
BstMAI GTCTC 2 cut(s) 294, 581
BstMBI GATC 8 cut(s) 71, 346, 370, 424, 478, 768, 859, 1015
BstMWI GCNNNNNNNGC 1 cut(s) 805
BstNI CCWGG 3 cut(s) 122, 692, 987
BstNSI RCATGY 3 cut(s) 487, 898, 959
BstPI GGTNACC 1 cut(s) 124
BstSCI CCNGG 3 cut(s) 120, 690, 985
BstV1I GCAGC 2 cut(s) 29, 205
BstX2I RGATCY 4 cut(s) 71, 346, 370, 1015
BstXI CCANNNNNNTGG 3 cut(s) 658, 1004, 1032
BstYI RGATCY 4 cut(s) 71, 346, 370, 1015
Bsu36I CCTNAGG 1 cut(s) 331
BsuRI GGCC 2 cut(s) 635, 690
BtrI CACGTC 1 cut(s) 941
BtsCI GGATG 5 cut(s) 427, 445, 466, 616, 982
BtsIMutI CAGTG 1 cut(s) 684
CfoI GCGC 1 cut(s) 168
Cfr13I GGNCC 1 cut(s) 634
CseI GACGC 1 cut(s) 626
CviAII CATG 4 cut(s) 484, 658, 895, 956
DdeI CTNAG 5 cut(s) 103, 331, 350, 621, 915
DpnI GATC 8 cut(s) 73, 348, 372, 426, 480, 770, 861, 1017
DpnII GATC 8 cut(s) 71, 346, 370, 424, 478, 768, 859, 1015
DraI TTTAAA 3 cut(s) 528, 594, 951
EaeI YGGCCR 1 cut(s) 688
Eam1104I CTCTTC 1 cut(s) 385
EarI CTCTTC 1 cut(s) 385
Ecl136II GAGCTC 1 cut(s) 664
Eco24I GRGCYC 1 cut(s) 666
Eco53kI GAGCTC 1 cut(s) 664
Eco81I CCTNAGG 1 cut(s) 331
Eco88I CYCGRG 1 cut(s) 817
Eco91I GGTNACC 1 cut(s) 124
EcoICRI GAGCTC 1 cut(s) 664
EcoO65I GGTNACC 1 cut(s) 124
EcoRI GAATTC 1 cut(s) 905
EcoRII CCWGG 3 cut(s) 120, 690, 985
EcoT38I GRGCYC 1 cut(s) 666
FaeI CATG 4 cut(s) 487, 661, 898, 959
FalI AAGNNNNNCTT 2 cut(s) 867, 899
FatI CATG 4 cut(s) 483, 657, 894, 955
FauI CCCGC 1 cut(s) 507
Fnu4HI GCNGC 3 cut(s) 43, 219, 536
FokI GGATG 5 cut(s) 434, 452, 473, 623, 969
FriOI GRGCYC 1 cut(s) 666
Fsp4HI GCNGC 3 cut(s) 43, 219, 536
FspBI CTAG 2 cut(s) 243, 728
GlaI GCGC 1 cut(s) 167
GluI GCNGC 3 cut(s) 43, 219, 536
HaeIII GGCC 2 cut(s) 635, 690
HapII CCGG 1 cut(s) 492
HgaI GACGC 1 cut(s) 626
HhaI GCGC 1 cut(s) 168
Hin1II CATG 4 cut(s) 487, 661, 898, 959
Hin6I GCGC 1 cut(s) 166
HinP1I GCGC 1 cut(s) 166
HincII GTYRAC 2 cut(s) 196, 676
HindII GTYRAC 2 cut(s) 196, 676
HindIII AAGCTT 2 cut(s) 83, 566
HinfI GANTC 4 cut(s) 559, 917, 963, 1008
HpaII CCGG 1 cut(s) 492
HphI GGTGA 3 cut(s) 136, 791, 869
Hpy166II GTNNAC 5 cut(s) 28, 152, 196, 304, 676
Hpy188I TCNGA 6 cut(s) 294, 414, 707, 828, 922, 1003
Hpy188III TCNNGA 8 cut(s) 322, 470, 641, 721, 802, 817, 819, 863
Hpy8I GTNNAC 5 cut(s) 28, 152, 196, 304, 676
Hpy99I CGWCG 1 cut(s) 942
HpyAV CCTTC 4 cut(s) 348, 518, 647, 778
HpyCH4III ACNGT 2 cut(s) 25, 63
HpyCH4IV ACGT 1 cut(s) 940
HpyCH4V TGCA 4 cut(s) 186, 221, 405, 487
HpyF10VI GCNNNNNNNGC 1 cut(s) 805
HpyF3I CTNAG 5 cut(s) 103, 331, 350, 621, 915
HpySE526I ACGT 1 cut(s) 940
Hsp92II CATG 4 cut(s) 487, 661, 898, 959
HspAI GCGC 1 cut(s) 166
Kzo9I GATC 8 cut(s) 71, 346, 370, 424, 478, 768, 859, 1015
LmnI GCTCC 4 cut(s) 9, 215, 500, 661
Lsp1109I GCAGC 2 cut(s) 29, 205
LweI GCATC 2 cut(s) 601, 991
MaeI CTAG 2 cut(s) 243, 728
MaeII ACGT 1 cut(s) 940
MaeIII GTNAC 4 cut(s) 124, 276, 547, 837
MalI GATC 8 cut(s) 73, 348, 372, 426, 480, 770, 861, 1017
MboI GATC 8 cut(s) 71, 346, 370, 424, 478, 768, 859, 1015
MboII GAAGA 4 cut(s) 365, 395, 402, 887
MflI RGATCY 4 cut(s) 71, 346, 370, 1015
MhlI GDGCHC 1 cut(s) 666
MlsI TGGCCA 1 cut(s) 690
MluNI TGGCCA 1 cut(s) 690
MlyI GAGTC 2 cut(s) 926, 972
MmeI TCCRAC 3 cut(s) 75, 538, 940
MnlI CCTC 5 cut(s) 451, 513, 665, 937, 1029
Mox20I TGGCCA 1 cut(s) 690
MroXI GAANNNNTTC 1 cut(s) 882
MscI TGGCCA 1 cut(s) 690
MseI TTAA 6 cut(s) 111, 297, 527, 593, 737, 950
MslI CAYNNNNRTG 2 cut(s) 122, 1030
Msp20I TGGCCA 1 cut(s) 690
MspI CCGG 1 cut(s) 492
MspR9I CCNGG 3 cut(s) 122, 692, 987
MvaI CCWGG 3 cut(s) 122, 692, 987
MwoI GCNNNNNNNGC 1 cut(s) 805
NdeII GATC 8 cut(s) 71, 346, 370, 424, 478, 768, 859, 1015
NlaIII CATG 4 cut(s) 487, 661, 898, 959
NlaIV GGNNCC 1 cut(s) 496
NmeAIII GCCGAG 1 cut(s) 958
NmuCI GTSAC 1 cut(s) 124
NspI RCATGY 3 cut(s) 487, 898, 959
OliI CACNNNNGTG 1 cut(s) 122
PaeR7I CTCGAG 1 cut(s) 817
PdmI GAANNNNTTC 1 cut(s) 882
PfeI GAWTC 2 cut(s) 559, 1008
PflMI CCANNNNNTGG 2 cut(s) 175, 697
PfoI TCCNGGA 1 cut(s) 985
PkrI GCNGC 3 cut(s) 44, 220, 537
PleI GAGTC 2 cut(s) 925, 971
PpsI GAGTC 2 cut(s) 925, 971
Psp124BI GAGCTC 1 cut(s) 666
Psp6I CCWGG 3 cut(s) 120, 690, 985
PspEI GGTNACC 1 cut(s) 124
PspGI CCWGG 3 cut(s) 120, 690, 985
PspN4I GGNNCC 1 cut(s) 496
PspPI GGNCC 1 cut(s) 634
PsuI RGATCY 4 cut(s) 71, 346, 370, 1015
RseI CAYNNNNRTG 2 cut(s) 122, 1030
SacI GAGCTC 1 cut(s) 666
SaqAI TTAA 6 cut(s) 111, 297, 527, 593, 737, 950
SatI GCNGC 3 cut(s) 43, 219, 536
Sau3AI GATC 8 cut(s) 71, 346, 370, 424, 478, 768, 859, 1015
Sau96I GGNCC 1 cut(s) 634
SchI GAGTC 2 cut(s) 926, 972
ScrFI CCNGG 3 cut(s) 122, 692, 987
SduI GDGCHC 1 cut(s) 666
SfaNI GCATC 2 cut(s) 601, 991
Sfr274I CTCGAG 1 cut(s) 817
SlaI CTCGAG 1 cut(s) 817
SmiMI CAYNNNNRTG 2 cut(s) 122, 1030
SmlI CTYRAG 2 cut(s) 800, 817
SmoI CTYRAG 2 cut(s) 800, 817
SpeI ACTAGT 1 cut(s) 727
SsiI CCGC 2 cut(s) 500, 535
SspMI CTAG 2 cut(s) 243, 728
SstI GAGCTC 1 cut(s) 666
StyD4I CCNGG 3 cut(s) 120, 690, 985
TaaI ACNGT 2 cut(s) 25, 63
TaiI ACGT 1 cut(s) 943
TaqI TCGA 3 cut(s) 431, 666, 818
TauI GCSGC 1 cut(s) 538
TfiI GAWTC 2 cut(s) 559, 1008
Tru1I TTAA 6 cut(s) 111, 297, 527, 593, 737, 950
Tru9I TTAA 6 cut(s) 111, 297, 527, 593, 737, 950
TscAI CASTG 1 cut(s) 691
TseFI GTSAC 1 cut(s) 124
TseI GCWGC 2 cut(s) 42, 218
Tsp45I GTSAC 1 cut(s) 124
TspDTI ATGAA 6 cut(s) 365, 477, 726, 898, 918, 959
TspRI CASTG 1 cut(s) 691
Van91I CCANNNNNTGG 2 cut(s) 175, 697
XapI RAATTY 6 cut(s) 271, 317, 749, 763, 829, 905
XceI RCATGY 3 cut(s) 487, 898, 959
XcmI CCANNNNNNNNNTGG 1 cut(s) 415
XhoI CTCGAG 1 cut(s) 817
XmnI GAANNNNTTC 1 cut(s) 882
XspI CTAG 2 cut(s) 243, 728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.