Rw5G017760

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000865
Physical Location & Seq
Reverse (-)
22674275 .. 22677495
3221 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G017760.1

Sequence Viewer

Length: 2823 bp
ATGGCCGCGGATATTGCCGTTTCAGCAGCATTGGCATTAGTTGAGAAGGTCACAAGCTTCGTCGACAAAATACGCAAAAGTCCAAGCTATGTCCGTGAAGCTCTTAATAAGGCAAGAACTTGTTTGGAGAGGATGGATGCCTATCTAACGACGTCCCAAGCACGGATTGATCAAGGCAATCAAGTGCAGCTTCAAGCTCGTGTTAAACAGGTCCGAGATGCAGTTCATTACATTGAGGATGCACTTGACGAGTTCACAATTCAGGTTCGCCATCACTCCCAGTATACCCATTGGTATTCTGGGATGACACACTGCGCTGCTCATTCCACGGCCTATTGGAAAGCAAGTAACAGATTGTCCTCCGAAATTGAAGATATTATTACAAATAAGATCGGTTTTCTTAGAGAACTCGATGGACTCACACAAGAAGGCCAACCAACTTCTAGCACAAGCCAGCAGCATGGAGGTACTTTGACCCCGCAAGTTCTTGAAGACGATGAAATAGTGGGCTTTGACGTGCCTAAACAAAAACTCATTGAACAGTTGATGAAAGGAGATCCAAAGCGTCTGACGATTTCCATTGTAGGTCCTGGCGGCTCCGGCAAAACCACTATCATGAGGAATGTTTACGACACTAAAAAGGTTCAAAGGAATTTTAATTGCCGAGCTTGGATCGATGTGTTGCGTCCTCTAAATCTCGAAAATGTGTTGCGCACCATGTTGAGCAGTTTTGATCCAAAAGGAAAACGGCAGGAACCAGAAGGAACAATTAATTGTCTAAAAGAAAAATTGAAACTACTTCTGCAGAAAAAGAAGTTTCTGGTTGTCTTGGATAATGTTTGGCGTAACGAGGATTTGGAGTCCATTGTAAATGCACTGCCCAATGGTTCCCCTGGGAGTAAAATACTCGTATCAACTCCTACGTCTGATGTTGCTTCTCATTCCTCCAATAACTACATCCATGACTTGAGTTGTGGCTTATCAAAGCAACATGCCCGGAATCTGTTCTGCAAGAAGGCTTTTCCAAATAATAATAATAATAACTGTCCACAGGAGCTTGAGGAGTGGGTTGAAAAATTCCTGAAGAGGTGTGACGGTTTGCCTTCTGCAATTTCAGCTGTTGGTGCCGATCTAGCAAACAAGCGGCATTCTCCAGTTGAGTGGAAGAAGGCATATGAAAGTTGGGGGTTGAGCTACAGGGATCGTCCAAGCTCGCTTAGAAGTTGGGAGTTGAGCTACAGGGATCTTCCAAGCCAGCTTAAAAGTTGTTTCTTGTACTTCAGCGTGTTTCCAGAAGACTACTCTATCAAACGTGAGAAGCTAATTCGCTTGTGGATAGCCGAAGGATTTGTGAAGCCAGAGAGTAGAAAAACAATGGAGGACGTTGCAGAATGGTATCTAAATGAACTGGTTGGTAGAAACTTGGTTAGCATCAGCAGCAAGGAAACTGATGGACAAGTAAGGAGGTGTCGTGTTTCGAATCTTGTTCACCACTTCATCATTTCTAAACCTGAGAATTTTCTCTGTGTTTTGAAAGCGAACCACAGTACTACTGATTCAGGTACAAAAATTCGACGCCTTTCTGTTCAAGATGACAACATCACTATATCAGGGTCTGCATATGATTTGAATGGTATTCGTACCTTGCTAGTATTTGGCCAGGAGAGTTCTAATAATTCCCGCCAGTTTGGAAATGTACTTAAACACTTCAAGTTTTTAAAGGTTTTAGATTTGCAAGGTACGCCTTTGAAGAAGTTTCCAAAAGGTGTTGTCGGTCTCACCCTCTTAAGGTATTTGAGCCTTAGGGAAACTAAGATTAAAAGAGTTCCAAGGTCCATTATCAAGCTTGGATTCTTGGAAACCTTAGATCTTAAACACACCCAGATAACCCGTTTGCCAAAAGAGATATGTAAACTCTCCAATCTGCGCCACCTCTTAGTCCATCACTGGGATGCTGCATCTAAAAATATCCAAGCGGTAGTGGTTTCTTCAGGAAATATTAAAGCCCTACAGTCCATACAAAAGTTGTCACTAATTGAGGTGAAAAGGAGAAACAATAGAAAGCTCATAAAAGCCTTGGGAGAGTTGGTAGATCTAAGGAAGTTGGGGCTGCAAGTTGATCTTGCAAGTCAAGAAGATGGAAGGCAATTATGTTCATCCATTCAGAAAATGAAACATCTTTCCATGCTTGATCTAAGATCAAAGAGCGGGGAAGTGTATCTATATTTGGATCATATGGAATCGCCGCCTAAGTATGTACAACGTCTACATTTGGAAGGGCTCCTAGACACGCTGCCGCGGTGGATCCCCAAGCTTGATAGTCTGTCCAAGATTGCTCTGAAGTGCTCAAAACTGAATGCTAGAGTGGAACCCCTTGAGGCCCTTGAGTCTTTGCCTAATCTGATGGAGCTCGACTTGGTTGATTATTCCAAAGGGGAAGTGTTGAAATTCAAAGCTGGAACGTTTAAAGAACTAATTCTAGTAAGTATAGAACAATTTGATCAGCTAAGAGAGATGGTCATCGAGAATGGGGCGATGCCTAAGCTTAAGAAGCTAACCATATCCAAATGTCTCAATCTGGAGTCAGTTCCAAAGGGCATCGAGGGTCTCACATCCCTCGACCAACTGTGTGTAAATGGCATGCACACGACCTTCATTACTCACTTGAAAAGCATAGCGAACAGAAGCAGCTGGAACATTTTTCGACGCAATGAAAAAAAAAATCTCATGCTTATGCACACATTACTTAATCGGATTGACGTCAAAGAATTTTCCACTCAAAGGTACAAGCGTCTCCGTGATTCTTTACGTTGGGTGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

940

Amino Acids

107.14

Weight (kDa)

9.48

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 94 2.8e-10 Rx N-terminal domain
NB-ARC PF00931 174 - 344 3.3e-33 NB-ARC domain
WHD_DRP PF23559 429 - 500 7.8e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 547 - 878 5e-48 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 155, 2763
Acc16I TGCGCA 1 cut(s) 713
AccB1I GGYRCC 1 cut(s) 1124
AccBSI CCGCTC 1 cut(s) 2208
AccI GTMKAC 3 cut(s) 63, 284, 2266
AccII CGCG 2 cut(s) 8, 2299
AclI AACGTT 1 cut(s) 2462
AclWI GGATC 8 cut(s) 551, 680, 728, 1209, 1251, 2238, 2299, 2312
AcoI YGGCCR 2 cut(s) 3, 1657
AcsI RAATTY 6 cut(s) 652, 1076, 1516, 1569, 2447, 2768
AcuI CTGAAG 4 cut(s) 1103, 1264, 1974, 2360
AcyI GRCGYC 3 cut(s) 152, 1576, 2760
AfaI GTAC 9 cut(s) 469, 1277, 1549, 1564, 1642, 1698, 1741, 2259, 2786
AfiI CCNNNNNNNGG 4 cut(s) 162, 1788, 1948, 2781
AflII CTTAAG 2 cut(s) 1786, 2546
AjiI CACGTC 1 cut(s) 517
AjnI CCWGG 3 cut(s) 589, 892, 1659
Alw21I GWGCWC 2 cut(s) 2348, 2412
Alw26I GTCTC 4 cut(s) 1781, 2576, 2612, 2798
AlwI GGATC 8 cut(s) 551, 680, 728, 1209, 1251, 2238, 2299, 2312
AlwNI CAGNNNCTG 1 cut(s) 1616
AoxI GGCC 5 cut(s) 3, 330, 430, 1657, 2379
ApeKI GCWGC 9 cut(s) 26, 187, 317, 457, 1437, 1955, 2110, 2293, 2688
ApoI RAATTY 6 cut(s) 652, 1076, 1516, 1569, 2447, 2768
AseI ATTAAT 1 cut(s) 771
Asp700I GAANNNNTTC 2 cut(s) 1004, 2475
AspLEI GCGC 3 cut(s) 317, 714, 1929
AspS9I GGNCC 4 cut(s) 211, 587, 1833, 2380
AsuC2I CCSGG 1 cut(s) 997
AsuHPI GGTGA 3 cut(s) 1481, 1771, 2053
AsuII TTCGAA 1 cut(s) 1478
AvaII GGWCC 3 cut(s) 211, 587, 1833
AxyI CCTNAGG 1 cut(s) 1802
BaeI ACNNNNGTAYC 2 cut(s) 2776, 2809
BalI TGGCCA 1 cut(s) 1659
BamHI GGATCC 1 cut(s) 2304
BanI GGYRCC 1 cut(s) 1124
BanII GRGCYC 2 cut(s) 2283, 2412
BauI CACGAG 1 cut(s) 198
BbsI GAAGAC 2 cut(s) 498, 1302
Bbv12I GWGCWC 2 cut(s) 2348, 2412
BbvI GCAGC 9 cut(s) 38, 199, 304, 469, 1449, 1942, 2097, 2280, 2700
BccI CCATC 8 cut(s) 127, 279, 407, 1445, 1950, 2132, 2398, 2509
BceAI ACGGC 3 cut(s) 2, 345, 764
BciT130I CCWGG 3 cut(s) 591, 894, 1661
BclI TGATCA 2 cut(s) 169, 2500
BcnI CCSGG 1 cut(s) 997
BcoDI GTCTC 4 cut(s) 1781, 2576, 2612, 2798
BfaI CTAG 6 cut(s) 444, 1133, 1649, 2285, 2361, 2480
BfmI CTRYAG 4 cut(s) 803, 1195, 1237, 2009
BfrI CTTAAG 2 cut(s) 1786, 2546
BglII AGATCT 2 cut(s) 1867, 2092
BmcAI AGTACT 1 cut(s) 1549
Bme1390I CCNGG 4 cut(s) 591, 894, 997, 1661
Bme18I GGWCC 3 cut(s) 211, 587, 1833
BmgBI CACGTC 1 cut(s) 517
BmgT120I GGNCC 4 cut(s) 211, 587, 1833, 2380
BmiI GGNNCC 7 cut(s) 598, 756, 889, 1126, 2282, 2306, 2370
BmrFI CCNGG 4 cut(s) 591, 894, 997, 1661
BmrI ACTGGG 2 cut(s) 274, 1957
BmsI GCATC 8 cut(s) 127, 208, 229, 1440, 1942, 1967, 2526, 2607
BmuI ACTGGG 2 cut(s) 274, 1957
BpiI GAAGAC 2 cut(s) 498, 1302
BpmI CTGGAG 2 cut(s) 1137, 2600
Bpu10I CCTNAGC 1 cut(s) 2541
Bpu14I TTCGAA 1 cut(s) 1478
BpuEI CTTGAG 4 cut(s) 988, 1079, 2396, 2405
BpuMI CCSGG 1 cut(s) 997
Bsa29I ATCGAT 1 cut(s) 675
BsaBI GATNNNNATC 2 cut(s) 141, 2519
BsaHI GRCGYC 3 cut(s) 152, 1576, 2760
BsaI GGTCTC 2 cut(s) 1781, 2612
BsaJI CCNNGG 7 cut(s) 6, 327, 892, 893, 1829, 2076, 2297
BsaXI ACNNNNNCTCC 4 cut(s) 1456, 1486, 2073, 2103
Bsc4I CCNNNNNNNGG 4 cut(s) 162, 1788, 1948, 2781
Bse1I ACTGG 5 cut(s) 280, 1154, 1413, 1684, 1952
Bse21I CCTNAGG 1 cut(s) 1802
Bse3DI GCAATG 1 cut(s) 2716
Bse8I GATNNNNATC 2 cut(s) 141, 2519
BseBI CCWGG 3 cut(s) 591, 894, 1661
BseCI ATCGAT 1 cut(s) 675
BseDI CCNNGG 7 cut(s) 6, 327, 892, 893, 1829, 2076, 2297
BseGI GGATG 8 cut(s) 138, 142, 244, 309, 957, 1957, 2156, 2612
BseJI GATNNNNATC 2 cut(s) 141, 2519
BseLI CCNNNNNNNGG 4 cut(s) 162, 1788, 1948, 2781
BseMI GCAATG 1 cut(s) 2716
BseMII CTCAG 1 cut(s) 1503
BseNI ACTGG 5 cut(s) 280, 1154, 1413, 1684, 1952
BseRI GAGGAG 1 cut(s) 1076
BseXI GCAGC 9 cut(s) 38, 199, 304, 469, 1449, 1942, 2097, 2280, 2700
BsgI GTGCAG 1 cut(s) 206
Bsh1236I CGCG 2 cut(s) 8, 2299
BshFI GGCC 5 cut(s) 5, 332, 432, 1659, 2381
BshNI GGYRCC 1 cut(s) 1124
BshVI ATCGAT 1 cut(s) 675
BsiHKAI GWGCWC 2 cut(s) 2348, 2412
BsiSI CCGG 2 cut(s) 600, 997
BslFI GGGAC 1 cut(s) 139
BslI CCNNNNNNNGG 4 cut(s) 162, 1788, 1948, 2781
BsmAI GTCTC 4 cut(s) 1781, 2576, 2612, 2798
BsmBI CGTCTC 1 cut(s) 2798
BsmFI GGGAC 1 cut(s) 139
BsmI GAATGC 2 cut(s) 1147, 2362
BsnI GGCC 5 cut(s) 5, 332, 432, 1659, 2381
Bso31I GGTCTC 2 cut(s) 1781, 2612
Bsp119I TTCGAA 1 cut(s) 1478
Bsp1286I GDGCHC 3 cut(s) 2283, 2348, 2412
Bsp1407I TGTACA 1 cut(s) 2257
BspANI GGCC 5 cut(s) 5, 332, 432, 1659, 2381
BspCNI CTCAG 1 cut(s) 1504
BspDI ATCGAT 1 cut(s) 675
BspFNI CGCG 2 cut(s) 8, 2299
BspHI TCATGA 1 cut(s) 615
BspLI GGNNCC 7 cut(s) 598, 756, 889, 1126, 2282, 2306, 2370
BspMAI CTGCAG 1 cut(s) 807
BspPI GGATC 8 cut(s) 551, 680, 728, 1209, 1251, 2238, 2299, 2312
BspT104I TTCGAA 1 cut(s) 1478
BspT107I GGYRCC 1 cut(s) 1124
BspTI CTTAAG 2 cut(s) 1786, 2546
BspTNI GGTCTC 2 cut(s) 1781, 2612
BsrBI CCGCTC 1 cut(s) 2208
BsrDI GCAATG 1 cut(s) 2716
BsrGI TGTACA 1 cut(s) 2257
BsrI ACTGG 5 cut(s) 280, 1154, 1413, 1684, 1952
BssECI CCNNGG 7 cut(s) 6, 327, 892, 893, 1829, 2076, 2297
BssNAI GTATAC 1 cut(s) 285
BssNI GRCGYC 3 cut(s) 152, 1576, 2760
BssSI CACGAG 1 cut(s) 198
BssT1I CCWWGG 2 cut(s) 1829, 2076
Bst1107I GTATAC 1 cut(s) 285
Bst2BI CACGAG 1 cut(s) 198
Bst2UI CCWGG 3 cut(s) 591, 894, 1661
Bst4CI ACNGT 6 cut(s) 543, 1046, 1097, 1547, 2013, 2628
Bst6I CTCTTC 1 cut(s) 1079
BstACI GRCGYC 3 cut(s) 152, 1576, 2760
BstAFI CTTAAG 2 cut(s) 1786, 2546
BstAUI TGTACA 1 cut(s) 2257
BstBI TTCGAA 1 cut(s) 1478
BstC8I GCNNGC 4 cut(s) 455, 1214, 1256, 2642
BstDSI CCRYGG 3 cut(s) 6, 327, 2297
BstF5I GGATG 8 cut(s) 138, 142, 244, 309, 957, 1957, 2156, 2612
BstFNI CGCG 2 cut(s) 8, 2299
BstHHI GCGC 3 cut(s) 317, 714, 1929
BstMAI GTCTC 4 cut(s) 1781, 2576, 2612, 2798
BstNI CCWGG 3 cut(s) 591, 894, 1661
BstNSI RCATGY 2 cut(s) 995, 2644
BstSCI CCNGG 4 cut(s) 589, 892, 995, 1659
BstSFI CTRYAG 4 cut(s) 803, 1195, 1237, 2009
BstUI CGCG 2 cut(s) 8, 2299
BstV1I GCAGC 9 cut(s) 38, 199, 304, 469, 1449, 1942, 2097, 2280, 2700
BstV2I GAAGAC 2 cut(s) 498, 1302
BstX2I RGATCY 5 cut(s) 556, 1243, 1867, 2092, 2304
BstXI CCANNNNNNTGG 2 cut(s) 461, 1161
BstYI RGATCY 5 cut(s) 556, 1243, 1867, 2092, 2304
BstZ17I GTATAC 1 cut(s) 285
Bsu15I ATCGAT 1 cut(s) 675
Bsu36I CCTNAGG 1 cut(s) 1802
BsuRI GGCC 5 cut(s) 5, 332, 432, 1659, 2381
BsuTUI ATCGAT 1 cut(s) 675
BtgI CCRYGG 3 cut(s) 6, 327, 2297
BtgZI GCGATG 1 cut(s) 2549
BtrI CACGTC 1 cut(s) 517
BtsCI GGATG 8 cut(s) 138, 142, 244, 309, 957, 1957, 2156, 2612
BtsI GCAGTG 2 cut(s) 310, 875
BtsIMutI CAGTG 3 cut(s) 310, 875, 1945
Cac8I GCNNGC 4 cut(s) 455, 1214, 1256, 2642
CaiI CAGNNNCTG 1 cut(s) 1616
CciI TCATGA 1 cut(s) 615
CfoI GCGC 3 cut(s) 317, 714, 1929
Cfr13I GGNCC 4 cut(s) 211, 587, 1833, 2380
Cfr42I CCGCGG 2 cut(s) 9, 2300
ClaI ATCGAT 1 cut(s) 675
CseI GACGC 5 cut(s) 554, 674, 1584, 2715, 2780
Csp6I GTAC 9 cut(s) 468, 1276, 1548, 1563, 1641, 1697, 1740, 2258, 2785
CviAII CATG 8 cut(s) 461, 616, 718, 962, 992, 2185, 2641, 2728
CviQI GTAC 9 cut(s) 468, 1276, 1548, 1563, 1641, 1697, 1740, 2258, 2785
DraI TTTAAA 2 cut(s) 1719, 2467
EaeI YGGCCR 2 cut(s) 3, 1657
Eam1104I CTCTTC 1 cut(s) 1079
EarI CTCTTC 1 cut(s) 1079
Ecl136II GAGCTC 1 cut(s) 2410
Eco130I CCWWGG 2 cut(s) 1829, 2076
Eco24I GRGCYC 2 cut(s) 2283, 2412
Eco31I GGTCTC 2 cut(s) 1781, 2612
Eco47I GGWCC 3 cut(s) 211, 587, 1833
Eco53kI GAGCTC 1 cut(s) 2410
Eco57I CTGAAG 4 cut(s) 1103, 1264, 1974, 2360
Eco81I CCTNAGG 1 cut(s) 1802
EcoICRI GAGCTC 1 cut(s) 2410
EcoO109I RGGNCCY 2 cut(s) 587, 2380
EcoRII CCWGG 3 cut(s) 589, 892, 1659
EcoT14I CCWWGG 2 cut(s) 1829, 2076
EcoT38I GRGCYC 2 cut(s) 2283, 2412
ErhI CCWWGG 2 cut(s) 1829, 2076
Esp3I CGTCTC 1 cut(s) 2798
FaeI CATG 8 cut(s) 464, 619, 721, 965, 995, 2188, 2644, 2731
FalI AAGNNNNNCTT 4 cut(s) 174, 206, 2106, 2138
FaqI GGGAC 1 cut(s) 139
FatI CATG 8 cut(s) 460, 615, 717, 961, 991, 2184, 2640, 2727
FauI CCCGC 3 cut(s) 486, 1688, 2201
FauNDI CATATG 3 cut(s) 1174, 1621, 2235
FbaI TGATCA 2 cut(s) 169, 2500
FblI GTMKAC 3 cut(s) 63, 284, 2266
FokI GGATG 8 cut(s) 145, 149, 251, 316, 944, 1964, 2143, 2599
FriOI GRGCYC 2 cut(s) 2283, 2412
FspBI CTAG 6 cut(s) 444, 1133, 1649, 2285, 2361, 2480
FspI TGCGCA 1 cut(s) 713
GlaI GCGC 3 cut(s) 316, 713, 1928
GsuI CTGGAG 2 cut(s) 1137, 2600
HaeIII GGCC 5 cut(s) 5, 332, 432, 1659, 2381
HapII CCGG 2 cut(s) 600, 997
HgaI GACGC 5 cut(s) 554, 674, 1584, 2715, 2780
HhaI GCGC 3 cut(s) 317, 714, 1929
Hin1I GRCGYC 3 cut(s) 152, 1576, 2760
Hin1II CATG 8 cut(s) 464, 619, 721, 965, 995, 2188, 2644, 2731
Hin6I GCGC 3 cut(s) 315, 712, 1927
HinP1I GCGC 3 cut(s) 315, 712, 1927
HincII GTYRAC 1 cut(s) 64
HindII GTYRAC 1 cut(s) 64
HindIII AAGCTT 4 cut(s) 55, 1844, 2312, 2543
HpaII CCGG 2 cut(s) 600, 997
HphI GGTGA 3 cut(s) 1481, 1771, 2053
Hpy166II GTNNAC 8 cut(s) 64, 255, 285, 628, 1049, 1489, 1913, 2267
Hpy188I TCNGA 8 cut(s) 215, 364, 570, 928, 2166, 2340, 2403, 2754
Hpy8I GTNNAC 8 cut(s) 64, 255, 285, 628, 1049, 1489, 1913, 2267
Hpy99I CGWCG 4 cut(s) 65, 154, 1578, 2709
HpyCH4III ACNGT 6 cut(s) 543, 1046, 1097, 1547, 2013, 2628
HpyCH4IV ACGT 9 cut(s) 152, 516, 923, 1312, 1383, 2263, 2462, 2760, 2809
HpySE526I ACGT 9 cut(s) 152, 516, 923, 1312, 1383, 2263, 2462, 2760, 2809
Hsp92I GRCGYC 3 cut(s) 152, 1576, 2760
Hsp92II CATG 8 cut(s) 464, 619, 721, 965, 995, 2188, 2644, 2731
HspAI GCGC 3 cut(s) 315, 712, 1927
Ksp22I TGATCA 2 cut(s) 169, 2500
KspI CCGCGG 2 cut(s) 9, 2300
LmnI GCTCC 4 cut(s) 602, 1054, 2286, 2407
Lsp1109I GCAGC 9 cut(s) 38, 199, 304, 469, 1449, 1942, 2097, 2280, 2700
LweI GCATC 8 cut(s) 127, 208, 229, 1440, 1942, 1967, 2526, 2607
MaeI CTAG 6 cut(s) 444, 1133, 1649, 2285, 2361, 2480
MaeII ACGT 9 cut(s) 152, 516, 923, 1312, 1383, 2263, 2462, 2760, 2809
MaeIII GTNAC 5 cut(s) 49, 347, 845, 1091, 2028
MbiI CCGCTC 1 cut(s) 2208
MboII GAAGA 9 cut(s) 383, 503, 1096, 1177, 1238, 1307, 1762, 1980, 2147
MflI RGATCY 5 cut(s) 556, 1243, 1867, 2092, 2304
MhlI GDGCHC 3 cut(s) 2283, 2348, 2412
MlsI TGGCCA 1 cut(s) 1659
MluNI TGGCCA 1 cut(s) 1659
MlyI GAGTC 4 cut(s) 411, 869, 2396, 2591
Mox20I TGGCCA 1 cut(s) 1659
MroXI GAANNNNTTC 2 cut(s) 1004, 2475
MscI TGGCCA 1 cut(s) 1659
MslI CAYNNNNRTG 3 cut(s) 614, 1950, 2732
Msp20I TGGCCA 1 cut(s) 1659
MspA1I CMGCKG 4 cut(s) 8, 1118, 2299, 2691
MspCI CTTAAG 2 cut(s) 1786, 2546
MspI CCGG 2 cut(s) 600, 997
MspR9I CCNGG 4 cut(s) 591, 894, 997, 1661
Mva1269I GAATGC 2 cut(s) 1147, 2362
MvaI CCWGG 3 cut(s) 591, 894, 1661
MvnI CGCG 2 cut(s) 8, 2299
NciI CCSGG 1 cut(s) 997
NdeI CATATG 3 cut(s) 1174, 1621, 2235
NlaIII CATG 8 cut(s) 464, 619, 721, 965, 995, 2188, 2644, 2731
NlaIV GGNNCC 7 cut(s) 598, 756, 889, 1126, 2282, 2306, 2370
NmeAIII GCCGAG 1 cut(s) 689
NmuCI GTSAC 3 cut(s) 49, 1091, 2028
NsbI TGCGCA 1 cut(s) 713
NspI RCATGY 2 cut(s) 995, 2644
NspV TTCGAA 1 cut(s) 1478
PaeI GCATGC 1 cut(s) 2644
PagI TCATGA 1 cut(s) 615
PasI CCCWGGG 1 cut(s) 893
PctI GAATGC 2 cut(s) 1147, 2362
PdmI GAANNNNTTC 2 cut(s) 1004, 2475
PfeI GAWTC 6 cut(s) 1000, 1480, 1556, 1851, 2240, 2801
PleI GAGTC 4 cut(s) 411, 868, 2395, 2590
PpsI GAGTC 4 cut(s) 411, 868, 2395, 2590
PpuMI RGGWCCY 1 cut(s) 587
PshBI ATTAAT 1 cut(s) 771
Psp124BI GAGCTC 1 cut(s) 2412
Psp1406I AACGTT 1 cut(s) 2462
Psp5II RGGWCCY 1 cut(s) 587
Psp6I CCWGG 3 cut(s) 589, 892, 1659
PspGI CCWGG 3 cut(s) 589, 892, 1659
PspN4I GGNNCC 7 cut(s) 598, 756, 889, 1126, 2282, 2306, 2370
PspPI GGNCC 4 cut(s) 211, 587, 1833, 2380
PspPPI RGGWCCY 1 cut(s) 587
PstI CTGCAG 1 cut(s) 807
PstNI CAGNNNCTG 1 cut(s) 1616
PsuI RGATCY 5 cut(s) 556, 1243, 1867, 2092, 2304
PvuII CAGCTG 2 cut(s) 1118, 2691
RsaI GTAC 9 cut(s) 469, 1277, 1549, 1564, 1642, 1698, 1741, 2259, 2786
RsaNI GTAC 9 cut(s) 468, 1276, 1548, 1563, 1641, 1697, 1740, 2258, 2785
RseI CAYNNNNRTG 3 cut(s) 614, 1950, 2732
SacI GAGCTC 1 cut(s) 2412
SacII CCGCGG 2 cut(s) 9, 2300
SalI GTCGAC 1 cut(s) 62
Sau96I GGNCC 4 cut(s) 211, 587, 1833, 2380
ScaI AGTACT 1 cut(s) 1549
SchI GAGTC 4 cut(s) 411, 869, 2396, 2591
ScrFI CCNGG 4 cut(s) 591, 894, 997, 1661
SduI GDGCHC 3 cut(s) 2283, 2348, 2412
SfaNI GCATC 8 cut(s) 127, 208, 229, 1440, 1942, 1967, 2526, 2607
SfcI CTRYAG 4 cut(s) 803, 1195, 1237, 2009
Sfr303I CCGCGG 2 cut(s) 9, 2300
SfuI TTCGAA 1 cut(s) 1478
SgrBI CCGCGG 2 cut(s) 9, 2300
SinI GGWCC 3 cut(s) 211, 587, 1833
SmiMI CAYNNNNRTG 3 cut(s) 614, 1950, 2732
SmlI CTYRAG 6 cut(s) 967, 1058, 1786, 2375, 2384, 2546
SmoI CTYRAG 6 cut(s) 967, 1058, 1786, 2375, 2384, 2546
SphI GCATGC 1 cut(s) 2644
SspI AATATT 1 cut(s) 1999
SspMI CTAG 6 cut(s) 444, 1133, 1649, 2285, 2361, 2480
SstI GAGCTC 1 cut(s) 2412
StyD4I CCNGG 4 cut(s) 589, 892, 995, 1659
StyI CCWWGG 2 cut(s) 1829, 2076
TaaI ACNGT 6 cut(s) 543, 1046, 1097, 1547, 2013, 2628
TaiI ACGT 9 cut(s) 155, 519, 926, 1315, 1386, 2266, 2465, 2763, 2812
TaqII GACCGA 1 cut(s) 1763
TatI WGTACW 4 cut(s) 1275, 1547, 1696, 2257
TauI GCSGC 5 cut(s) 8, 597, 1147, 2248, 2299
TfiI GAWTC 6 cut(s) 1000, 1480, 1556, 1851, 2240, 2801
TscAI CASTG 3 cut(s) 317, 882, 1952
TseFI GTSAC 3 cut(s) 49, 1091, 2028
TseI GCWGC 9 cut(s) 26, 187, 317, 457, 1437, 1955, 2110, 2293, 2688
Tsp45I GTSAC 3 cut(s) 49, 1091, 2028
TspGWI ACGGA 3 cut(s) 83, 178, 2786
TspRI CASTG 3 cut(s) 317, 882, 1952
Vha464I CTTAAG 2 cut(s) 1786, 2546
VpaK11BI GGWCC 3 cut(s) 211, 587, 1833
VspI ATTAAT 1 cut(s) 771
XapI RAATTY 6 cut(s) 652, 1076, 1516, 1569, 2447, 2768
XceI RCATGY 2 cut(s) 995, 2644
XcmI CCANNNNNNNNNTGG 1 cut(s) 296
XmiI GTMKAC 3 cut(s) 63, 284, 2266
XmnI GAANNNNTTC 2 cut(s) 1004, 2475
XspI CTAG 6 cut(s) 444, 1133, 1649, 2285, 2361, 2480
ZraI GACGTC 2 cut(s) 153, 2761
ZrmI AGTACT 1 cut(s) 1549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.