RchiOBHm_Chr2g0168051

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000865
Physical Location & Seq
Forward (+)
82521674 .. 82523179
1506 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53577

Sequence Viewer

Length: 1506 bp
ATGGCTGATGCTATTTCAGCCGCATTGCTGTTAGTTGAGACGGCGATAATCTGCACCGACCAATTACACAGTCGTACGAGTAGTGTCCGTAAGGATCTTGAAAGGGCCCGGAATTGTTTGGCGAGGTTGCAACAGTGGGATAATCACACAAATCCCCAAGCTAGGGAGAAACAGGTCCAAGATTTGGCTTGTAATATTGAGGATGCTATTAACGAGTACCTGCTTCAGGTTTCACAGCATTCCCATACCAATCGGTTCTCTAAGCTGGCGCACAACGTTGGTCATTTCATCCCCGACTGGATAGCAAGCAGTAAGTTATCCTCCAGCATTGATGAACTGGAACGAGACCTGAATTTGGTTACGTTACTAGATTCAGTACATTCAAATGGTCCCCAAGAAGGCCGCCCAAGCTCCAGCACAGGACCTCAGGGGGCAACTTTGACCCACAGATTTCTTGAAGACCATGAAATAGTGGGCTATGATGAAGAGAAAACACGACTTTTCCAACACTTGGGAGAAAGAAATCCAGAGCTTTTGACAATTGTGATTGTAGGTCCTGGAGGCTCCGGCAAAACTACACTCGTAAAGAATGTTTACCAAAGCGAAAGGGTCAGCAGCTTGTTTCATTTTCAAGCTTGGATTGATGTGCCACTCGATCTTGACCAACTCCTGCCGAAGTTGTTAAGAAGATTTAAGCAGGAAGTAGATCCTCATGAAGATATCAGTGACCTTCAAGAGAAACTGAGAAGAATTTTGGAGAATAAGAAGTTTTTGGTTGTCCTGGATAATGTTTGGAGCCACGAAGCTTTCGAATCCATTGTAAATGCACTACCAAAAACGGAGGGTAGTAAACTAATCGTAATTACTCGGAACATTGATGTAGCTCCGGTTTCTTCCAATTATATTCATGATTTGTGTGCTGGGTTATCATCGAAGAAAGCCAACCAGCTATTCTGCAAGAAGGCCTTTCCTGATGTTGTACGAAATATTTGTCCAACCCATCTGGAGGACTGGGCTGAACAGATTGTAACGAGGTGTGAAGGTATGCCCTGGGCAATTTCACAAATCGGCACTTTGCTAGCAAGGACGCCGAGAGTAGCAGTTGAGTGGAAGAAGATGCATGACACACTAGGATCCGATCTCTCCATTGCTAGCAGTATTTCACATTATAGGGATCTGCCAAGCAATCACAAAGAATGTTTCCTGTACTTCGGTATGTTTCCAGAAGACGAAACCATCAGCCGAGAGAGGCTAATACGGTTGTGGATAGCCGAAGGATTTGTGAGACCAGACGGAGTAAAAACGGTGGAGGCAGTTGCACAGACTATTCTGACTAATCTCATTGACAGAAATTTGGTTGTGGTCAGTGCGAGGGAAATCGATGGACGAGTCAAGAATTGTCGTGTTTCCAACCTTGTTCGTGAGTTCATCATTTCCAAAGCAAAGCATTTCATCACTGTTTTTGGAAGCCAAGAGTGTTGGGAATATTGTTCGGGATTCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

501

Amino Acids

56.81

Weight (kDa)

6.19

Isoelectric Point (pI)

43.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_16 PF13191 158 - 280 5.4e-06 AAA ATPase domain
NB-ARC PF00931 162 - 325 1.4e-26 NB-ARC domain
AAA_22 PF13401 181 - 264 2e-06 AAA domain
WHD_DRP PF23559 406 - 477 1.4e-17 Disease resistance protein Winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 228
AccB7I CCANNNNNTGG 2 cut(s) 184, 511
AciI CCGC 2 cut(s) 21, 403
AclI AACGTT 1 cut(s) 276
AclWI GGATC 5 cut(s) 102, 701, 1128, 1141, 1182
AcsI RAATTY 3 cut(s) 352, 750, 1351
AcuI CTGAAG 1 cut(s) 209
AcyI GRCGYC 1 cut(s) 1088
AfaI GTAC 5 cut(s) 76, 218, 378, 981, 1208
AfiI CCNNNNNNNGG 8 cut(s) 162, 163, 184, 226, 355, 398, 511, 1006
AgsI TTSAA 5 cut(s) 101, 384, 458, 632, 734
AjnI CCWGG 3 cut(s) 556, 780, 1049
AjuI GAANNNNNNNTTGG 2 cut(s) 935, 967
AluBI AGCT 9 cut(s) 161, 265, 411, 532, 618, 635, 806, 884, 949
AluI AGCT 9 cut(s) 161, 265, 411, 532, 618, 635, 806, 884, 949
Alw26I GTCTC 3 cut(s) 32, 339, 1279
AlwI GGATC 5 cut(s) 102, 701, 1128, 1141, 1182
AoxI GGCC 3 cut(s) 105, 400, 963
ApaI GGGCCC 1 cut(s) 109
ApeKI GCWGC 1 cut(s) 615
ApoI RAATTY 3 cut(s) 352, 750, 1351
ArsI GACNNNNNNTTYG 2 cut(s) 976, 1008
AspLEI GCGC 1 cut(s) 271
AspS9I GGNCC 6 cut(s) 105, 106, 175, 389, 422, 554
AsuC2I CCSGG 1 cut(s) 109
AsuII TTCGAA 1 cut(s) 810
AsuNHI GCTAGC 2 cut(s) 1078, 1151
AvaII GGWCC 4 cut(s) 175, 389, 422, 554
AxyI CCTNAGG 1 cut(s) 426
BaeGI GKGCMC 1 cut(s) 109
BamHI GGATCC 1 cut(s) 1133
BanII GRGCYC 1 cut(s) 109
BbsI GAAGAC 2 cut(s) 465, 1233
BbvI GCAGC 1 cut(s) 627
BccI CCATC 3 cut(s) 1008, 1244, 1376
BceAI ACGGC 1 cut(s) 57
BciT130I CCWGG 3 cut(s) 558, 782, 1051
BcnI CCSGG 1 cut(s) 109
BcoDI GTCTC 3 cut(s) 32, 339, 1279
BfaI CTAG 5 cut(s) 162, 368, 1079, 1130, 1152
BfuAI ACCTGC 1 cut(s) 228
BisI GCNGC 3 cut(s) 21, 403, 616
BlsI GCNGC 3 cut(s) 22, 404, 617
Bme1390I CCNGG 4 cut(s) 109, 558, 782, 1051
Bme18I GGWCC 4 cut(s) 175, 389, 422, 554
BmgT120I GGNCC 6 cut(s) 105, 106, 175, 389, 422, 554
BmiI GGNNCC 5 cut(s) 107, 391, 565, 797, 1135
BmrFI CCNGG 4 cut(s) 109, 558, 782, 1051
BmrI ACTGGG 1 cut(s) 1021
BmsI GCATC 2 cut(s) 193, 1107
BmtI GCTAGC 2 cut(s) 1082, 1155
BmuI ACTGGG 1 cut(s) 1021
BpiI GAAGAC 2 cut(s) 465, 1233
BpmI CTGGAG 4 cut(s) 307, 397, 579, 1025
Bpu14I TTCGAA 1 cut(s) 810
BpuMI CCSGG 1 cut(s) 109
Bsa29I ATCGAT 1 cut(s) 1380
BsaHI GRCGYC 1 cut(s) 1088
BsaI GGTCTC 2 cut(s) 339, 1279
BsaJI CCNNGG 2 cut(s) 1049, 1050
BsaWI WCCGGW 1 cut(s) 886
Bsc4I CCNNNNNNNGG 8 cut(s) 162, 163, 184, 226, 355, 398, 511, 1006
Bse1I ACTGG 3 cut(s) 302, 342, 1016
Bse21I CCTNAGG 1 cut(s) 426
Bse3DI GCAATG 2 cut(s) 23, 1146
BseBI CCWGG 3 cut(s) 558, 782, 1051
BseCI ATCGAT 1 cut(s) 1380
BseDI CCNNGG 2 cut(s) 1049, 1050
BseGI GGATG 2 cut(s) 208, 288
BseLI CCNNNNNNNGG 8 cut(s) 162, 163, 184, 226, 355, 398, 511, 1006
BseMI GCAATG 2 cut(s) 23, 1146
BseMII CTCAG 2 cut(s) 440, 734
BseNI ACTGG 3 cut(s) 302, 342, 1016
BseSI GKGCMC 1 cut(s) 109
BseXI GCAGC 1 cut(s) 627
BseYI CCCAGC 1 cut(s) 920
BsgI GTGCAG 1 cut(s) 37
BshFI GGCC 3 cut(s) 107, 402, 965
BshVI ATCGAT 1 cut(s) 1380
BsiSI CCGG 3 cut(s) 109, 567, 887
BsiWI CGTACG 1 cut(s) 74
BslFI GGGAC 1 cut(s) 375
BslI CCNNNNNNNGG 8 cut(s) 162, 163, 184, 226, 355, 398, 511, 1006
BsmAI GTCTC 3 cut(s) 32, 339, 1279
BsmBI CGTCTC 1 cut(s) 32
BsmFI GGGAC 1 cut(s) 375
BsmI GAATGC 1 cut(s) 238
BsnI GGCC 3 cut(s) 107, 402, 965
Bso31I GGTCTC 2 cut(s) 339, 1279
Bsp119I TTCGAA 1 cut(s) 810
Bsp120I GGGCCC 1 cut(s) 105
Bsp1286I GDGCHC 1 cut(s) 109
Bsp143I GATC 6 cut(s) 94, 655, 706, 1133, 1138, 1174
BspACI CCGC 2 cut(s) 21, 403
BspANI GGCC 3 cut(s) 107, 402, 965
BspCNI CTCAG 2 cut(s) 439, 735
BspDI ATCGAT 1 cut(s) 1380
BspHI TCATGA 2 cut(s) 712, 907
BspLI GGNNCC 5 cut(s) 107, 391, 565, 797, 1135
BspMI ACCTGC 1 cut(s) 228
BspOI GCTAGC 2 cut(s) 1082, 1155
BspPI GGATC 5 cut(s) 102, 701, 1128, 1141, 1182
BspT104I TTCGAA 1 cut(s) 810
BspTNI GGTCTC 2 cut(s) 339, 1279
BsrDI GCAATG 2 cut(s) 23, 1146
BsrI ACTGG 3 cut(s) 302, 342, 1016
BssECI CCNNGG 2 cut(s) 1049, 1050
BssMI GATC 6 cut(s) 94, 655, 706, 1133, 1138, 1174
BssNI GRCGYC 1 cut(s) 1088
Bst2UI CCWGG 3 cut(s) 558, 782, 1051
Bst4CI ACNGT 5 cut(s) 71, 135, 1260, 1306, 1459
Bst6I CTCTTC 1 cut(s) 480
BstACI GRCGYC 1 cut(s) 1088
BstBI TTCGAA 1 cut(s) 810
BstC8I GCNNGC 4 cut(s) 267, 307, 1080, 1153
BstDEI CTNAG 3 cut(s) 261, 426, 743
BstENI CCTNNNNNAGG 1 cut(s) 224
BstF5I GGATG 2 cut(s) 208, 288
BstHHI GCGC 1 cut(s) 271
BstKTI GATC 6 cut(s) 97, 658, 709, 1136, 1141, 1177
BstMAI GTCTC 3 cut(s) 32, 339, 1279
BstMBI GATC 6 cut(s) 94, 655, 706, 1133, 1138, 1174
BstMWI GCNNNNNNNGC 2 cut(s) 17, 408
BstNI CCWGG 3 cut(s) 558, 782, 1051
BstSCI CCNGG 4 cut(s) 107, 556, 780, 1049
BstSLI GKGCMC 1 cut(s) 109
BstV1I GCAGC 1 cut(s) 627
BstV2I GAAGAC 2 cut(s) 465, 1233
BstX2I RGATCY 4 cut(s) 94, 706, 1133, 1174
BstYI RGATCY 4 cut(s) 94, 706, 1133, 1174
Bsu15I ATCGAT 1 cut(s) 1380
Bsu36I CCTNAGG 1 cut(s) 426
BsuRI GGCC 3 cut(s) 107, 402, 965
BsuTUI ATCGAT 1 cut(s) 1380
BtsCI GGATG 2 cut(s) 208, 288
BtsIMutI CAGTG 4 cut(s) 140, 730, 1372, 1455
BveI ACCTGC 1 cut(s) 228
Cac8I GCNNGC 4 cut(s) 267, 307, 1080, 1153
CciI TCATGA 2 cut(s) 712, 907
CfoI GCGC 1 cut(s) 271
Cfr13I GGNCC 6 cut(s) 105, 106, 175, 389, 422, 554
ClaI ATCGAT 1 cut(s) 1380
CseI GACGC 1 cut(s) 1096
Csp6I GTAC 5 cut(s) 75, 217, 377, 980, 1207
CviAII CATG 4 cut(s) 464, 713, 908, 1121
CviQI GTAC 5 cut(s) 75, 217, 377, 980, 1207
DdeI CTNAG 3 cut(s) 261, 426, 743
DpnI GATC 6 cut(s) 96, 657, 708, 1135, 1140, 1176
DpnII GATC 6 cut(s) 94, 655, 706, 1133, 1138, 1174
Eam1104I CTCTTC 1 cut(s) 480
EarI CTCTTC 1 cut(s) 480
Eco147I AGGCCT 1 cut(s) 965
Eco24I GRGCYC 1 cut(s) 109
Eco31I GGTCTC 2 cut(s) 339, 1279
Eco32I GATATC 1 cut(s) 721
Eco47I GGWCC 4 cut(s) 175, 389, 422, 554
Eco57I CTGAAG 1 cut(s) 209
Eco81I CCTNAGG 1 cut(s) 426
EcoNI CCTNNNNNAGG 1 cut(s) 224
EcoO109I RGGNCCY 3 cut(s) 105, 422, 554
EcoRII CCWGG 3 cut(s) 556, 780, 1049
EcoRV GATATC 1 cut(s) 721
EcoT22I ATGCAT 1 cut(s) 1122
EcoT38I GRGCYC 1 cut(s) 109
Esp3I CGTCTC 1 cut(s) 32
FaeI CATG 4 cut(s) 467, 716, 911, 1124
FalI AAGNNNNNCTT 2 cut(s) 950, 982
FaqI GGGAC 1 cut(s) 375
FatI CATG 4 cut(s) 463, 712, 907, 1120
Fnu4HI GCNGC 3 cut(s) 21, 403, 616
FokI GGATG 2 cut(s) 215, 275
FriOI GRGCYC 1 cut(s) 109
Fsp4HI GCNGC 3 cut(s) 21, 403, 616
FspBI CTAG 5 cut(s) 162, 368, 1079, 1130, 1152
GlaI GCGC 1 cut(s) 270
GluI GCNGC 3 cut(s) 21, 403, 616
GsaI CCCAGC 1 cut(s) 924
GsuI CTGGAG 4 cut(s) 307, 397, 579, 1025
HaeIII GGCC 3 cut(s) 107, 402, 965
HapII CCGG 3 cut(s) 109, 567, 887
HgaI GACGC 1 cut(s) 1096
HhaI GCGC 1 cut(s) 271
Hin1I GRCGYC 1 cut(s) 1088
Hin1II CATG 4 cut(s) 467, 716, 911, 1124
Hin6I GCGC 1 cut(s) 269
HinP1I GCGC 1 cut(s) 269
HindIII AAGCTT 2 cut(s) 633, 804
HinfI GANTC 4 cut(s) 371, 812, 1389, 1497
HpaII CCGG 3 cut(s) 109, 567, 887
Hpy166II GTNNAC 2 cut(s) 595, 851
Hpy188I TCNGA 3 cut(s) 870, 1138, 1332
Hpy8I GTNNAC 2 cut(s) 595, 851
HpyAV CCTTC 5 cut(s) 392, 740, 955, 1034, 1268
HpyCH4III ACNGT 5 cut(s) 71, 135, 1260, 1306, 1459
HpyCH4IV ACGT 2 cut(s) 276, 362
HpyCH4V TGCA 6 cut(s) 54, 130, 827, 957, 1120, 1319
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 408
HpyF3I CTNAG 3 cut(s) 261, 426, 743
HpySE526I ACGT 2 cut(s) 276, 362
Hsp92I GRCGYC 1 cut(s) 1088
Hsp92II CATG 4 cut(s) 467, 716, 911, 1124
HspAI GCGC 1 cut(s) 269
Kzo9I GATC 6 cut(s) 94, 655, 706, 1133, 1138, 1174
LmnI GCTCC 4 cut(s) 416, 569, 795, 889
Lsp1109I GCAGC 1 cut(s) 627
LweI GCATC 2 cut(s) 193, 1107
MaeI CTAG 5 cut(s) 162, 368, 1079, 1130, 1152
MaeII ACGT 2 cut(s) 276, 362
MaeIII GTNAC 4 cut(s) 358, 363, 725, 1027
MalI GATC 6 cut(s) 96, 657, 708, 1135, 1140, 1176
MboI GATC 6 cut(s) 94, 655, 706, 1133, 1138, 1174
MfeI CAATTG 1 cut(s) 540
MflI RGATCY 4 cut(s) 94, 706, 1133, 1174
MhlI GDGCHC 1 cut(s) 109
MlyI GAGTC 1 cut(s) 1398
MmeI TCCRAC 3 cut(s) 529, 1019, 1434
Mph1103I ATGCAT 1 cut(s) 1122
MseI TTAA 3 cut(s) 210, 683, 693
MslI CAYNNNNRTG 1 cut(s) 384
MspI CCGG 3 cut(s) 109, 567, 887
MspR9I CCNGG 4 cut(s) 109, 558, 782, 1051
MunI CAATTG 1 cut(s) 540
Mva1269I GAATGC 1 cut(s) 238
MvaI CCWGG 3 cut(s) 558, 782, 1051
MwoI GCNNNNNNNGC 2 cut(s) 17, 408
NciI CCSGG 1 cut(s) 109
NdeII GATC 6 cut(s) 94, 655, 706, 1133, 1138, 1174
NheI GCTAGC 2 cut(s) 1078, 1151
NlaIII CATG 4 cut(s) 467, 716, 911, 1124
NlaIV GGNNCC 5 cut(s) 107, 391, 565, 797, 1135
NmeAIII GCCGAG 2 cut(s) 1116, 1268
NmuCI GTSAC 1 cut(s) 725
NsiI ATGCAT 1 cut(s) 1122
NspV TTCGAA 1 cut(s) 810
PagI TCATGA 2 cut(s) 712, 907
PasI CCCWGGG 1 cut(s) 1050
PceI AGGCCT 1 cut(s) 965
PcsI WCGNNNNNNNCGW 1 cut(s) 807
PctI GAATGC 1 cut(s) 238
PfeI GAWTC 3 cut(s) 371, 812, 1497
Pfl23II CGTACG 1 cut(s) 74
PflMI CCANNNNNTGG 2 cut(s) 184, 511
PfoI TCCNGGA 2 cut(s) 556, 780
PkrI GCNGC 3 cut(s) 22, 404, 617
PleI GAGTC 1 cut(s) 1397
PpsI GAGTC 1 cut(s) 1397
PpuMI RGGWCCY 2 cut(s) 422, 554
Psp1406I AACGTT 1 cut(s) 276
Psp5II RGGWCCY 2 cut(s) 422, 554
Psp6I CCWGG 3 cut(s) 556, 780, 1049
PspFI CCCAGC 1 cut(s) 920
PspGI CCWGG 3 cut(s) 556, 780, 1049
PspLI CGTACG 1 cut(s) 74
PspN4I GGNNCC 5 cut(s) 107, 391, 565, 797, 1135
PspOMI GGGCCC 1 cut(s) 105
PspPI GGNCC 6 cut(s) 105, 106, 175, 389, 422, 554
PspPPI RGGWCCY 2 cut(s) 422, 554
PsuI RGATCY 4 cut(s) 94, 706, 1133, 1174
RsaI GTAC 5 cut(s) 76, 218, 378, 981, 1208
RsaNI GTAC 5 cut(s) 75, 217, 377, 980, 1207
RseI CAYNNNNRTG 1 cut(s) 384
SaqAI TTAA 3 cut(s) 210, 683, 693
SatI GCNGC 3 cut(s) 21, 403, 616
Sau3AI GATC 6 cut(s) 94, 655, 706, 1133, 1138, 1174
Sau96I GGNCC 6 cut(s) 105, 106, 175, 389, 422, 554
SchI GAGTC 1 cut(s) 1398
ScrFI CCNGG 4 cut(s) 109, 558, 782, 1051
SduI GDGCHC 1 cut(s) 109
SfaNI GCATC 2 cut(s) 193, 1107
SfuI TTCGAA 1 cut(s) 810
SinI GGWCC 4 cut(s) 175, 389, 422, 554
SmiMI CAYNNNNRTG 1 cut(s) 384
SseBI AGGCCT 1 cut(s) 965
SsiI CCGC 2 cut(s) 21, 403
SspI AATATT 3 cut(s) 196, 988, 1487
SspMI CTAG 5 cut(s) 162, 368, 1079, 1130, 1152
StuI AGGCCT 1 cut(s) 965
StyD4I CCNGG 4 cut(s) 107, 556, 780, 1049
TaaI ACNGT 5 cut(s) 71, 135, 1260, 1306, 1459
TaiI ACGT 2 cut(s) 279, 365
TaqI TCGA 4 cut(s) 654, 810, 932, 1380
TatI WGTACW 2 cut(s) 376, 1206
TauI GCSGC 2 cut(s) 23, 405
TfiI GAWTC 3 cut(s) 371, 812, 1497
Tru1I TTAA 3 cut(s) 210, 683, 693
Tru9I TTAA 3 cut(s) 210, 683, 693
TscAI CASTG 4 cut(s) 140, 730, 1372, 1462
TseFI GTSAC 1 cut(s) 725
TseI GCWGC 1 cut(s) 615
Tsp45I GTSAC 1 cut(s) 725
TspDTI ATGAA 9 cut(s) 277, 348, 480, 498, 614, 729, 896, 1417, 1441
TspGWI ACGGA 3 cut(s) 77, 854, 1308
TspRI CASTG 4 cut(s) 140, 730, 1372, 1462
Van91I CCANNNNNTGG 2 cut(s) 184, 511
VpaK11BI GGWCC 4 cut(s) 175, 389, 422, 554
XagI CCTNNNNNAGG 1 cut(s) 224
XapI RAATTY 3 cut(s) 352, 750, 1351
XspI CTAG 5 cut(s) 162, 368, 1079, 1130, 1152
Zsp2I ATGCAT 1 cut(s) 1122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.