Rh5AG196300

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000865
Physical Location & Seq
Forward (+)
22870631 .. 22873447
2817 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG196300.1

Sequence Viewer

Length: 1062 bp
ATGGCTGAACTTGCCATTCCTGTAGCACTGGCCTTAATTGACAAGGTGATAATCATCCTCCAGCAAAACTTTCACCGTCAAACTAGAGTCAAGGATGATGTTGAAAAAGCCAAGAATTGGTTGAGGAGGATGGAAGCGTATCTAGCCGACACATCAGAACATAGCGATGAGGGTACTCGAGTGTTTCAGATTCGAGTAAGAGAAATCCAAGATTTGGTTTATGAAATTAAGGATGCTCTCGACAAGTTCATGCTTCAGGTTCCTCATCACATCCATAGCAATATAGCCTCTCAGACGGTACACAAAGCTGTTCATTATTTGCCAACCAGGAATGCAGGCAAGGAGTTGTCGACTAAGATTAATGGTATCGAGAAAAAAGTTCGTTGGATTACAGAACTAGATGCTCTTCATATCCAAGCTCCAGCACAAAGCAGCCCAAGTTCCAGTTCCGGGGAAGGAATGACCCACTACTACCATGTTCTTGAAGAAGAAGAAGAAATAGTGGGCTTCGAGGAGCATGAAGAAAAACTCTTGGCACAGTTGATGGAAGAAAATTCAAGGCTTTCGACGATTTCGATTGTAGGCCCGGGCGGTGCGGGCAAAACCACTCTGGCAAAGAAGGTTTTCGACAACAAGCGGGTCCAAGGGTTTTTTGACCGCCAAGCTTGGGTTGATGTGCCACGCGCATCTGTACCCCGTAAGGTTTTGCTAAACATTCTAAGCAAAATTGAGGCAAAAGGGCAGAAGGAACCAGTAATTCATGGAGAAAATGATCCAAAGGATAAATTGAGACTACTTCTCGAGGACAAGCGGTTTTTGGTCGTTTTGGATAATGTTTGGAACAGACAAGTTTTGGAATACGTCGTAAAGGCATTACAACACGGTTCGAATGGCAGTAGAATCATCATCACTACTCCTAAAGTTTACCATGGAAAAAAGCCAAGTACCTATTCTGCAAGAAGCAGCAAAGTTCTCATTCTGTGCTTGAAAAGTCTCGGCCGCAACCATTCTGTCACTGATTTCAAGAATCTTTCTCCATCACAGTGGCTTGTGGAACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

353

Amino Acids

40.16

Weight (kDa)

9.01

Isoelectric Point (pI)

51.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 11 - 93 1.9e-13 Rx N-terminal domain
NB-ARC PF00931 171 - 305 5.6e-29 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 117, 214
AccI GTMKAC 1 cut(s) 350
AccII CGCG 1 cut(s) 684
AciI CCGC 6 cut(s) 591, 596, 637, 658, 811, 1000
AclWI GGATC 1 cut(s) 767
AcoI YGGCCR 1 cut(s) 997
AcsI RAATTY 1 cut(s) 553
AcuI CTGAAG 1 cut(s) 239
AfaI GTAC 4 cut(s) 175, 300, 693, 946
AfiI CCNNNNNNNGG 4 cut(s) 117, 214, 450, 667
AgsI TTSAA 5 cut(s) 104, 485, 558, 988, 1024
AjnI CCWGG 1 cut(s) 326
AjuI GAANNNNNNNTTGG 4 cut(s) 430, 462, 934, 966
AluBI AGCT 3 cut(s) 308, 419, 665
AluI AGCT 3 cut(s) 308, 419, 665
Alw26I GTCTC 2 cut(s) 784, 998
AlwI GGATC 1 cut(s) 767
Ama87I CYCGRG 3 cut(s) 177, 586, 800
AoxI GGCC 3 cut(s) 30, 583, 997
ApeKI GCWGC 2 cut(s) 432, 963
ApoI RAATTY 1 cut(s) 553
AseI ATTAAT 1 cut(s) 360
Asp700I GAANNNNTTC 1 cut(s) 623
AspLEI GCGC 1 cut(s) 686
AspS9I GGNCC 2 cut(s) 584, 640
AsuC2I CCSGG 3 cut(s) 451, 587, 588
AsuHPI GGTGA 2 cut(s) 58, 65
AsuII TTCGAA 1 cut(s) 887
AvaI CYCGRG 3 cut(s) 177, 586, 800
AvaII GGWCC 1 cut(s) 640
BaeI ACNNNNGTAYC 2 cut(s) 165, 198
BbvI GCAGC 2 cut(s) 444, 975
BccI CCATC 3 cut(s) 124, 538, 1045
BciT130I CCWGG 1 cut(s) 328
BcnI CCSGG 3 cut(s) 451, 587, 588
BcoDI GTCTC 2 cut(s) 784, 998
BfaI CTAG 3 cut(s) 84, 143, 398
BfmI CTRYAG 1 cut(s) 21
BisI GCNGC 3 cut(s) 433, 964, 1000
BlsI GCNGC 3 cut(s) 434, 965, 1001
Bme1390I CCNGG 4 cut(s) 328, 451, 587, 588
Bme18I GGWCC 1 cut(s) 640
BmeT110I CYCGRG 3 cut(s) 177, 586, 800
BmgT120I GGNCC 2 cut(s) 584, 640
BmiI GGNNCC 3 cut(s) 261, 641, 750
BmrFI CCNGG 4 cut(s) 328, 451, 587, 588
BmsI GCATC 3 cut(s) 223, 391, 695
BpmI CTGGAG 2 cut(s) 44, 405
Bpu14I TTCGAA 1 cut(s) 887
BpuMI CCSGG 3 cut(s) 451, 587, 588
BsaBI GATNNNNATC 1 cut(s) 53
BsaJI CCNNGG 4 cut(s) 450, 586, 643, 928
Bsc4I CCNNNNNNNGG 4 cut(s) 117, 214, 450, 667
Bse1I ACTGG 3 cut(s) 33, 444, 752
Bse8I GATNNNNATC 1 cut(s) 53
BseBI CCWGG 1 cut(s) 328
BseDI CCNNGG 4 cut(s) 450, 586, 643, 928
BseGI GGATG 5 cut(s) 54, 100, 135, 238, 270
BseJI GATNNNNATC 1 cut(s) 53
BseLI CCNNNNNNNGG 4 cut(s) 117, 214, 450, 667
BseMII CTCAG 1 cut(s) 305
BseNI ACTGG 3 cut(s) 33, 444, 752
BseRI GAGGAG 2 cut(s) 139, 527
BseX3I CGGCCG 1 cut(s) 997
BseXI GCAGC 2 cut(s) 444, 975
Bsh1236I CGCG 1 cut(s) 684
Bsh1285I CGRYCG 1 cut(s) 1000
BshFI GGCC 3 cut(s) 32, 585, 999
BsiEI CGRYCG 1 cut(s) 1000
BsiHKCI CYCGRG 3 cut(s) 177, 586, 800
BsiSI CCGG 2 cut(s) 450, 587
BslI CCNNNNNNNGG 4 cut(s) 117, 214, 450, 667
BsmAI GTCTC 2 cut(s) 784, 998
BsmI GAATGC 1 cut(s) 337
BsnI GGCC 3 cut(s) 32, 585, 999
BsoBI CYCGRG 3 cut(s) 177, 586, 800
Bsp119I TTCGAA 1 cut(s) 887
Bsp143I GATC 1 cut(s) 772
Bsp19I CCATGG 1 cut(s) 928
BspACI CCGC 6 cut(s) 591, 596, 637, 658, 811, 1000
BspANI GGCC 3 cut(s) 32, 585, 999
BspCNI CTCAG 1 cut(s) 304
BspFNI CGCG 1 cut(s) 684
BspLI GGNNCC 3 cut(s) 261, 641, 750
BspPI GGATC 1 cut(s) 767
BspQI GCTCTTC 1 cut(s) 411
BspT104I TTCGAA 1 cut(s) 887
BsrI ACTGG 3 cut(s) 33, 444, 752
BssECI CCNNGG 4 cut(s) 450, 586, 643, 928
BssMI GATC 1 cut(s) 772
BssT1I CCWWGG 2 cut(s) 643, 928
Bst2UI CCWGG 1 cut(s) 328
Bst4CI ACNGT 5 cut(s) 77, 298, 540, 884, 1044
Bst6I CTCTTC 1 cut(s) 411
BstBI TTCGAA 1 cut(s) 887
BstC8I GCNNGC 2 cut(s) 337, 598
BstDEI CTNAG 3 cut(s) 291, 354, 719
BstDSI CCRYGG 1 cut(s) 928
BstF5I GGATG 5 cut(s) 54, 100, 135, 238, 270
BstFNI CGCG 1 cut(s) 684
BstHHI GCGC 1 cut(s) 686
BstKTI GATC 1 cut(s) 775
BstMAI GTCTC 2 cut(s) 784, 998
BstMBI GATC 1 cut(s) 772
BstMCI CGRYCG 1 cut(s) 1000
BstMWI GCNNNNNNNGC 3 cut(s) 11, 143, 597
BstNI CCWGG 1 cut(s) 328
BstSCI CCNGG 4 cut(s) 326, 449, 585, 586
BstSFI CTRYAG 1 cut(s) 21
BstUI CGCG 1 cut(s) 684
BstV1I GCAGC 2 cut(s) 444, 975
BstXI CCANNNNNNTGG 1 cut(s) 1044
BstZI CGGCCG 1 cut(s) 997
BsuRI GGCC 3 cut(s) 32, 585, 999
BtgI CCRYGG 1 cut(s) 928
BtgZI GCGATG 1 cut(s) 180
BtsCI GGATG 5 cut(s) 54, 100, 135, 238, 270
BtsIMutI CAGTG 3 cut(s) 26, 1014, 1049
Cac8I GCNNGC 2 cut(s) 337, 598
CfoI GCGC 1 cut(s) 686
Cfr13I GGNCC 2 cut(s) 584, 640
Cfr9I CCCGGG 1 cut(s) 586
Csp6I GTAC 4 cut(s) 174, 299, 692, 945
CviAII CATG 5 cut(s) 250, 476, 518, 761, 929
CviQI GTAC 4 cut(s) 174, 299, 692, 945
DdeI CTNAG 3 cut(s) 291, 354, 719
DpnI GATC 1 cut(s) 774
DpnII GATC 1 cut(s) 772
EaeI YGGCCR 1 cut(s) 997
EagI CGGCCG 1 cut(s) 997
Eam1104I CTCTTC 1 cut(s) 411
EarI CTCTTC 1 cut(s) 411
EclXI CGGCCG 1 cut(s) 997
Eco130I CCWWGG 2 cut(s) 643, 928
Eco47I GGWCC 1 cut(s) 640
Eco52I CGGCCG 1 cut(s) 997
Eco57I CTGAAG 1 cut(s) 239
Eco88I CYCGRG 3 cut(s) 177, 586, 800
EcoRII CCWGG 1 cut(s) 326
EcoT14I CCWWGG 2 cut(s) 643, 928
ErhI CCWWGG 2 cut(s) 643, 928
FaeI CATG 5 cut(s) 253, 479, 521, 764, 932
FatI CATG 5 cut(s) 249, 475, 517, 760, 928
FauI CCCGC 2 cut(s) 589, 630
FblI GTMKAC 1 cut(s) 350
Fnu4HI GCNGC 3 cut(s) 433, 964, 1000
FokI GGATG 5 cut(s) 41, 107, 142, 245, 257
Fsp4HI GCNGC 3 cut(s) 433, 964, 1000
FspBI CTAG 3 cut(s) 84, 143, 398
GlaI GCGC 1 cut(s) 685
GluI GCNGC 3 cut(s) 433, 964, 1000
GsuI CTGGAG 2 cut(s) 44, 405
HaeIII GGCC 3 cut(s) 32, 585, 999
HapII CCGG 2 cut(s) 450, 587
HhaI GCGC 1 cut(s) 686
Hin1II CATG 5 cut(s) 253, 479, 521, 764, 932
Hin6I GCGC 1 cut(s) 684
HinP1I GCGC 1 cut(s) 684
HincII GTYRAC 1 cut(s) 351
HindII GTYRAC 1 cut(s) 351
HindIII AAGCTT 1 cut(s) 663
HinfI GANTC 4 cut(s) 87, 190, 900, 1027
HpaII CCGG 2 cut(s) 450, 587
HphI GGTGA 2 cut(s) 58, 65
Hpy166II GTNNAC 3 cut(s) 301, 351, 925
Hpy188I TCNGA 3 cut(s) 157, 189, 294
Hpy188III TCNNGA 5 cut(s) 239, 370, 482, 800, 1024
Hpy8I GTNNAC 3 cut(s) 301, 351, 925
Hpy99I CGWCG 2 cut(s) 571, 866
HpyAV CCTTC 3 cut(s) 449, 613, 739
HpyCH4III ACNGT 5 cut(s) 77, 298, 540, 884, 1044
HpyCH4IV ACGT 1 cut(s) 861
HpyCH4V TGCA 2 cut(s) 335, 956
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 143, 597
HpyF3I CTNAG 3 cut(s) 291, 354, 719
HpySE526I ACGT 1 cut(s) 861
Hsp92II CATG 5 cut(s) 253, 479, 521, 764, 932
HspAI GCGC 1 cut(s) 684
Kzo9I GATC 1 cut(s) 772
LguI GCTCTTC 1 cut(s) 411
LmnI GCTCC 2 cut(s) 424, 514
Lsp1109I GCAGC 2 cut(s) 444, 975
LweI GCATC 3 cut(s) 223, 391, 695
MaeI CTAG 3 cut(s) 84, 143, 398
MaeII ACGT 1 cut(s) 861
MaeIII GTNAC 1 cut(s) 1012
MalI GATC 1 cut(s) 774
MboI GATC 1 cut(s) 772
MboII GAAGA 7 cut(s) 398, 497, 500, 503, 506, 533, 560
MluCI AATT 7 cut(s) 36, 115, 225, 553, 726, 756, 785
MlyI GAGTC 1 cut(s) 96
MmeI TCCRAC 1 cut(s) 365
MnlI CCTC 9 cut(s) 68, 117, 120, 163, 273, 298, 505, 724, 796
MroXI GAANNNNTTC 1 cut(s) 623
MseI TTAA 3 cut(s) 35, 228, 360
MslI CAYNNNNRTG 2 cut(s) 165, 1042
MspI CCGG 2 cut(s) 450, 587
MspR9I CCNGG 4 cut(s) 328, 451, 587, 588
Mva1269I GAATGC 1 cut(s) 337
MvaI CCWGG 1 cut(s) 328
MvnI CGCG 1 cut(s) 684
MwoI GCNNNNNNNGC 3 cut(s) 11, 143, 597
NciI CCSGG 3 cut(s) 451, 587, 588
NcoI CCATGG 1 cut(s) 928
NdeII GATC 1 cut(s) 772
NlaIII CATG 5 cut(s) 253, 479, 521, 764, 932
NlaIV GGNNCC 3 cut(s) 261, 641, 750
NmeAIII GCCGAG 1 cut(s) 975
NmuCI GTSAC 1 cut(s) 1012
NspV TTCGAA 1 cut(s) 887
PaeR7I CTCGAG 2 cut(s) 177, 800
PciSI GCTCTTC 1 cut(s) 411
PcsI WCGNNNNNNNCGW 1 cut(s) 572
PctI GAATGC 1 cut(s) 337
PdmI GAANNNNTTC 1 cut(s) 623
PfeI GAWTC 3 cut(s) 190, 900, 1027
PflMI CCANNNNNTGG 2 cut(s) 117, 214
PkrI GCNGC 3 cut(s) 434, 965, 1001
PleI GAGTC 1 cut(s) 95
PpsI GAGTC 1 cut(s) 95
PshBI ATTAAT 1 cut(s) 360
Psp6I CCWGG 1 cut(s) 326
PspGI CCWGG 1 cut(s) 326
PspN4I GGNNCC 3 cut(s) 261, 641, 750
PspPI GGNCC 2 cut(s) 584, 640
PspXI VCTCGAGB 1 cut(s) 177
RsaI GTAC 4 cut(s) 175, 300, 693, 946
RsaNI GTAC 4 cut(s) 174, 299, 692, 945
RseI CAYNNNNRTG 2 cut(s) 165, 1042
SalI GTCGAC 1 cut(s) 349
SapI GCTCTTC 1 cut(s) 411
SaqAI TTAA 3 cut(s) 35, 228, 360
SatI GCNGC 3 cut(s) 433, 964, 1000
Sau3AI GATC 1 cut(s) 772
Sau96I GGNCC 2 cut(s) 584, 640
SchI GAGTC 1 cut(s) 96
ScrFI CCNGG 4 cut(s) 328, 451, 587, 588
SetI ASST 9 cut(s) 48, 261, 310, 421, 624, 667, 705, 864, 950
SfaNI GCATC 3 cut(s) 223, 391, 695
SfcI CTRYAG 1 cut(s) 21
Sfr274I CTCGAG 2 cut(s) 177, 800
SfuI TTCGAA 1 cut(s) 887
SinI GGWCC 1 cut(s) 640
SlaI CTCGAG 2 cut(s) 177, 800
SmaI CCCGGG 1 cut(s) 588
SmiMI CAYNNNNRTG 2 cut(s) 165, 1042
SmlI CTYRAG 2 cut(s) 177, 800
SmoI CTYRAG 2 cut(s) 177, 800
SrfI GCCCGGGC 1 cut(s) 588
Sse9I AATT 7 cut(s) 36, 115, 225, 553, 726, 756, 785
SsiI CCGC 6 cut(s) 591, 596, 637, 658, 811, 1000
SspMI CTAG 3 cut(s) 84, 143, 398
StyD4I CCNGG 4 cut(s) 326, 449, 585, 586
StyI CCWWGG 2 cut(s) 643, 928
TaaI ACNGT 5 cut(s) 77, 298, 540, 884, 1044
TaiI ACGT 1 cut(s) 864
TasI AATT 7 cut(s) 36, 115, 225, 553, 726, 756, 785
TauI GCSGC 1 cut(s) 1002
TfiI GAWTC 3 cut(s) 190, 900, 1027
Tru1I TTAA 3 cut(s) 35, 228, 360
Tru9I TTAA 3 cut(s) 35, 228, 360
TscAI CASTG 3 cut(s) 33, 1021, 1049
TseFI GTSAC 1 cut(s) 1012
TseI GCWGC 2 cut(s) 432, 963
Tsp45I GTSAC 1 cut(s) 1012
TspDTI ATGAA 6 cut(s) 237, 238, 302, 398, 534, 749
TspMI CCCGGG 1 cut(s) 586
TspRI CASTG 3 cut(s) 33, 1021, 1049
Van91I CCANNNNNTGG 2 cut(s) 117, 214
VpaK11BI GGWCC 1 cut(s) 640
VspI ATTAAT 1 cut(s) 360
XapI RAATTY 1 cut(s) 553
XhoI CTCGAG 2 cut(s) 177, 800
XmaI CCCGGG 1 cut(s) 586
XmiI GTMKAC 1 cut(s) 350
XmnI GAANNNNTTC 1 cut(s) 623
XspI CTAG 3 cut(s) 84, 143, 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.