Rh5DG195800

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000865
Physical Location & Seq
Forward (+)
21715122 .. 21718297
3176 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG195800.1

Sequence Viewer

Length: 2676 bp
ATGGCTGAACTTGCAATTCCTTTATCATTGTTGGTGGCCGAGAAGGCGATGAGCTTGTGCTACCAAAGAAACGCGTCCAAGGACCCAAGTAATAATATCCATGATGATGTTGAAAGAGCCTGGAAATGTCTAAGGAGGATGCAAGCCTATCTAGGGGACTCGGAAGAGATTGATGAAGGCAGCCTAATGCTTCAAAATCGTGTCGCAGAGGTCCAAAGTCTGGCTTATGAAATAGAGGATGCCCTTGACAAGTTCATATCTCAGATTCCACATCATTTCCACAGCAACATATTCTCTCAGAAGCTGCACGACGTTGCGCACTATCGCGAAGAAAAGAGAGCAAGAGTTGAGTTATCCACCAAGATTAAAGACATTGAAAAGAAAATTAGTTCCTTCTCAACTTTGGATTCACTTCTTGAGCCACCAAGTAGTTCCAGCACAAGGAAGAGGGATAGTTTGATCCACCAAGTTCTTGAGGAAGATGAAATAGTAGGCCTTGAGGAGCCTAAAGAAAGACTCTCCAAACAGTTGATGGAAGAAGATTCAAGGCTCAAGACTATTTTGGTTGTAGGTCCTGGCGGCTCCGGCAAAACCACAGTTGTGAAGAATGTTTTTGACAGCACAATGGTCCAACGGTTTTTTGATTGCCATGCTTGGATCGACTTGTCACGGCCTCCTAAAGCAGAAGATCAGAAAGCTGAACTGATGCATCTGAAGGAAAAGAGATTTTTGGTTGTTTTGGATAATGTTTGGACAGCACAAGATCTGGAACTCATTACAAATGCTTTGCCAAGAGGATTGCCTGGGAGTAAAGTAATCATAACTACTCGCAACTCTGATGTAGCTTCTTCTCGTGTTGAGTCCTCCGAGTATATCCATGATTTGAGTAGCGGGTTATCATGGAAAAAAGGCTGGTCTCTACTTTGCAAGAAGGCTTTTAGAAAAACTGCAGGATATTGTCCACCTGAACTTGAAGAGTGGGCTAAAAAGATCCTGAAAAGGTGTGAAGGTCTGCCCCTTGCAATTTCAGCTGTTGCTACTTTGCTGGCAAAGAAGCCACAAACTCCAGTTGAGTGGAAGAAGTTACATGAAAGCCTTGGGTCTGAAATTAGACCTGACTCTAGTCTTTCGATTATTAGCCGTGTCTTGCAGCCAAGTTACATGCATCTTCCGAGCCATCTGAAGATTTGTTTTTTGTACTTCGGCATGTTTCCTGAAGACTATTCAATCAGCCGTGACAGGCTGATTCGGTTGTGGTTAGCCGAAGGATTTGTGGTGCCAGGAAGAGGACAAACAATGGAGGAGGCTGCAGAGAGTTATCTGAATGAACTCATTGGAAGAAATTTGGTACATGCTCCCACAAGGGAGATTAATGGTCGAGTAAGGAGCTGTCGTGTTTTGAACCTTGTTCGAGAGTTCATCATTTCAAAAGCAGAGAACTTCATCACGGTTTTGGATCCAAACTGCCCTATTTCTACTCGTTCAAGTGAGAAAATTCGACGTCTTTCAATTCAAAATGTCAATATCAATTTATTAAGGGGTAGAGACTTTAGTCATGTTCGTACCTTACTAGTGTTTGGAGAGGAGTGTTCCCCTTCTGAGCTTGACAATGTGTTTAAGAACTCTAAGTTTTTAAGGGTGTTAGATTTTCTAGGTGTACCTTTGAAGAACTTTCCTGACTGTGTTATTGGTCTCCCCCTCTTGAGGTACTTATGTCTGAGCCAAACTGATATTAAATCAGTTCCAAAGTTCATTGGCAATCTTTCATTCTTGCAAACCTTAGATCTTAAGGGCACACAGGTGACCAACTTACCAAAACAGATTTATGCGCTCCAGGATATGCGTCATCTTTTAGTTTATAGTTGTGATGTGACTTCTGGAGTGGCACAAGGAGTTCAGATTTCTGCAGGCAATATTGGAGCTTTATCTTCCCTACAGAAGTTATTTCTAATTAAGGTGAAAAAACAAAGAAAAGTAGTCCTTAAAGCCTTGGGAGAGTTAATTGCTCTCCGTAAGTTGGGGCTGGTAGACCTTGAAAGAGAACATGGAAGAGAATTGTGTTGTTCCATTCAAAAAATGCTAGAGCTTTCAACACTTGATGTACGATCAACAAGCGAAGATGAGTATCTTGATTTGGATCAACTGAACTTCCCTCCCGGGCCTCCCCGCTATCTCCAACGTCTCTGTTTGACAGGGCGCCTAGAAAGTCTACCACAGTGGATTCCCCAGCTTCACAGTCTAGCTCGGATTGCTTTGAAGTGGTCTAAACTGAATGCTGATGTCAACTCACTAGAGGCGCTCCAAGATTTGCCTAATCTGGTAGAGCTAGAGTTGGTGGGCTATTACACTGGGGATTCAATGAAGTTCAAAGCCAAAAAGTTCACGAAACTAAAGGTATTACGTATTGAGCAACTTGATCAGCTAGATATGGTGGTAGTCGAGGATGGAGCAATGCCCGAGCTTAAGAAGTTAACATTGTCTAGATGTCAGAATCTGAAGTTACTTCCATATGGCATTGATAGGCTTATCTTGCTTGAAGAATTGATTCTGTATGACATGCCCGAAGAATTCATTGCTAGGCTTCAGAAGGATAGCGAAGACCGTTACTTGGTTGAGAATCTTCGAGTCATCAAATCTTTCTATTCTGGGAGCACTGGATGCCAAAATCTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

891

Amino Acids

101.24

Weight (kDa)

8.12

Isoelectric Point (pI)

48.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 31 - 94 6e-09 Rx N-terminal domain
NB-ARC PF00931 167 - 226 1.1e-10 NB-ARC domain
nSTAND_NTPase5 PF25199 172 - 285 1.8e-07 Novel STAND NTPase 5
NB-ARC PF00931 237 - 317 2.9e-15 NB-ARC domain
WHD_DRP PF23559 403 - 474 9.1e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 519 - 848 1.3e-42 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1504
Acc16I TGCGCA 1 cut(s) 318
AccB1I GGYRCC 2 cut(s) 1276, 2196
AccB7I CCANNNNNTGG 1 cut(s) 220
AccI GTMKAC 2 cut(s) 2028, 2209
AccII CGCG 2 cut(s) 74, 327
AciI CCGC 3 cut(s) 579, 891, 2167
AclWI GGATC 6 cut(s) 454, 665, 985, 1451, 1464, 2145
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 3 cut(s) 1342, 1494, 2567
AcuI CTGAAG 5 cut(s) 734, 1202, 1236, 2516, 2567
AcyI GRCGYC 2 cut(s) 1501, 2197
AfaI GTAC 6 cut(s) 1199, 1350, 1564, 1659, 1709, 2103
AfiI CCNNNNNNNGG 7 cut(s) 153, 220, 441, 1286, 1704, 2017, 2608
AflII CTTAAG 2 cut(s) 1787, 2462
AflIII ACRYGT 1 cut(s) 72
AhlI ACTAGT 1 cut(s) 1570
AjnI CCWGG 5 cut(s) 119, 574, 802, 1279, 1833
AleI CACNNNNGTG 2 cut(s) 599, 1799
Alw21I GWGCWC 1 cut(s) 2654
Alw26I GTCTC 4 cut(s) 921, 1539, 1697, 2186
AlwI GGATC 6 cut(s) 454, 665, 985, 1451, 1464, 2145
AlwNI CAGNNNCTG 2 cut(s) 304, 2494
Ama87I CYCGRG 2 cut(s) 2156, 2456
AoxI GGCC 4 cut(s) 36, 493, 671, 2159
ApeKI GCWGC 4 cut(s) 180, 304, 1150, 1307
ApoI RAATTY 3 cut(s) 1342, 1494, 2567
AseI ATTAAT 1 cut(s) 1371
Asp700I GAANNNNTTC 1 cut(s) 2544
AspLEI GCGC 4 cut(s) 319, 1831, 2199, 2299
AspS9I GGNCC 5 cut(s) 82, 211, 572, 628, 2159
AsuC2I CCSGG 2 cut(s) 2157, 2158
AsuHPI GGTGA 2 cut(s) 1813, 1969
AvaI CYCGRG 2 cut(s) 2156, 2456
AvaII GGWCC 4 cut(s) 82, 211, 572, 628
BaeGI GKGCMC 1 cut(s) 1796
BaeI ACNNNNGTAYC 2 cut(s) 1699, 1732
BamHI GGATCC 1 cut(s) 1456
BanI GGYRCC 2 cut(s) 1276, 2196
BauI CACGAG 1 cut(s) 852
BbsI GAAGAC 2 cut(s) 1224, 2604
Bbv12I GWGCWC 1 cut(s) 2654
BbvI GCAGC 4 cut(s) 192, 291, 1162, 1294
BccI CCATC 3 cut(s) 526, 1185, 2438
BceAI ACGGC 3 cut(s) 686, 1125, 1218
BciT130I CCWGG 5 cut(s) 121, 576, 804, 1281, 1835
BclI TGATCA 1 cut(s) 2416
BcnI CCSGG 2 cut(s) 2157, 2158
BcoDI GTCTC 4 cut(s) 921, 1539, 1697, 2186
BcuI ACTAGT 1 cut(s) 1570
BfmI CTRYAG 4 cut(s) 948, 1308, 1905, 1934
BfoI RGCGCY 2 cut(s) 2200, 2300
BfrI CTTAAG 2 cut(s) 1787, 2462
BglI GCCNNNNNGGC 1 cut(s) 44
BglII AGATCT 2 cut(s) 763, 1783
BisI GCNGC 5 cut(s) 181, 305, 580, 1151, 1308
BlsI GCNGC 5 cut(s) 182, 306, 581, 1152, 1309
Bme1390I CCNGG 7 cut(s) 121, 576, 804, 1281, 1835, 2157, 2158
Bme18I GGWCC 4 cut(s) 82, 211, 572, 628
BmeT110I CYCGRG 2 cut(s) 2156, 2456
BmgT120I GGNCC 5 cut(s) 82, 211, 572, 628, 2159
BmiI GGNNCC 6 cut(s) 84, 504, 583, 1278, 1458, 2198
BmrFI CCNGG 7 cut(s) 121, 576, 804, 1281, 1835, 2157, 2158
BmrI ACTGGG 1 cut(s) 2358
BmsI GCATC 6 cut(s) 129, 229, 696, 718, 1174, 2648
BmuI ACTGGG 1 cut(s) 2358
BoxI GACNNNNGTC 2 cut(s) 1122, 1551
BpiI GAAGAC 2 cut(s) 1224, 2604
BpmI CTGGAG 3 cut(s) 1050, 1817, 1899
BpuEI CTTGAG 5 cut(s) 437, 494, 518, 536, 1723
BpuMI CCSGG 2 cut(s) 2157, 2158
BsaAI YACGTR 1 cut(s) 2402
BsaBI GATNNNNATC 2 cut(s) 2124, 2136
BsaHI GRCGYC 2 cut(s) 1501, 2197
BsaI GGTCTC 2 cut(s) 921, 1697
BsaJI CCNNGG 5 cut(s) 78, 803, 1096, 1989, 2156
BsaXI ACNNNNNCTCC 2 cut(s) 1378, 1408
Bsc4I CCNNNNNNNGG 7 cut(s) 153, 220, 441, 1286, 1704, 2017, 2608
Bse1I ACTGG 3 cut(s) 1067, 2353, 2659
Bse3DI GCAATG 2 cut(s) 2457, 2571
Bse8I GATNNNNATC 2 cut(s) 2124, 2136
BseBI CCWGG 5 cut(s) 121, 576, 804, 1281, 1835
BseDI CCNNGG 5 cut(s) 78, 803, 1096, 1989, 2156
BseGI GGATG 4 cut(s) 144, 244, 2449, 2663
BseJI GATNNNNATC 2 cut(s) 2124, 2136
BseLI CCNNNNNNNGG 7 cut(s) 153, 220, 441, 1286, 1704, 2017, 2608
BseMI GCAATG 2 cut(s) 2457, 2571
BseMII CTCAG 4 cut(s) 275, 311, 1590, 1709
BseNI ACTGG 3 cut(s) 1067, 2353, 2659
BseRI GAGGAG 3 cut(s) 515, 1316, 1598
BseSI GKGCMC 1 cut(s) 1796
BseXI GCAGC 4 cut(s) 192, 291, 1162, 1294
BseYI CCCAGC 1 cut(s) 2226
BsgI GTGCAG 1 cut(s) 290
Bsh1236I CGCG 2 cut(s) 74, 327
BshFI GGCC 4 cut(s) 38, 495, 673, 2161
BshNI GGYRCC 2 cut(s) 1276, 2196
BsiHKAI GWGCWC 1 cut(s) 2654
BsiHKCI CYCGRG 2 cut(s) 2156, 2456
BsiSI CCGG 2 cut(s) 585, 2157
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 7 cut(s) 153, 220, 441, 1286, 1704, 2017, 2608
BsmAI GTCTC 4 cut(s) 921, 1539, 1697, 2186
BsmBI CGTCTC 1 cut(s) 2186
BsmFI GGGAC 1 cut(s) 170
BsmI GAATGC 1 cut(s) 2278
BsnI GGCC 4 cut(s) 38, 495, 673, 2161
Bso31I GGTCTC 2 cut(s) 921, 1697
BsoBI CYCGRG 2 cut(s) 2156, 2456
Bsp1286I GDGCHC 2 cut(s) 1796, 2654
Bsp68I TCGCGA 1 cut(s) 327
BspACI CCGC 3 cut(s) 579, 891, 2167
BspANI GGCC 4 cut(s) 38, 495, 673, 2161
BspCNI CTCAG 4 cut(s) 274, 310, 1591, 1710
BspFNI CGCG 2 cut(s) 74, 327
BspLI GGNNCC 6 cut(s) 84, 504, 583, 1278, 1458, 2198
BspMAI CTGCAG 3 cut(s) 952, 1312, 1909
BspPI GGATC 6 cut(s) 454, 665, 985, 1451, 1464, 2145
BspT107I GGYRCC 2 cut(s) 1276, 2196
BspTI CTTAAG 2 cut(s) 1787, 2462
BspTNI GGTCTC 2 cut(s) 921, 1697
BsrDI GCAATG 2 cut(s) 2457, 2571
BsrI ACTGG 3 cut(s) 1067, 2353, 2659
BssECI CCNNGG 5 cut(s) 78, 803, 1096, 1989, 2156
BssNI GRCGYC 2 cut(s) 1501, 2197
BssSI CACGAG 1 cut(s) 852
BssT1I CCWWGG 3 cut(s) 78, 1096, 1989
Bst2BI CACGAG 1 cut(s) 852
Bst2UI CCWGG 5 cut(s) 121, 576, 804, 1281, 1835
Bst4CI ACNGT 8 cut(s) 528, 598, 636, 1450, 1682, 2217, 2237, 2603
Bst6I CTCTTC 5 cut(s) 159, 440, 969, 1279, 2044
BstACI GRCGYC 2 cut(s) 1501, 2197
BstAFI CTTAAG 2 cut(s) 1787, 2462
BstAPI GCANNNNNTGC 1 cut(s) 2658
BstBAI YACGTR 1 cut(s) 2402
BstC8I GCNNGC 3 cut(s) 144, 1047, 1909
BstDEI CTNAG 7 cut(s) 131, 261, 297, 1599, 1626, 1718, 1780
BstEII GGTNACC 1 cut(s) 1801
BstF5I GGATG 4 cut(s) 144, 244, 2449, 2663
BstFNI CGCG 2 cut(s) 74, 327
BstH2I RGCGCY 2 cut(s) 2200, 2300
BstHHI GCGC 4 cut(s) 319, 1831, 2199, 2299
BstMAI GTCTC 4 cut(s) 921, 1539, 1697, 2186
BstMWI GCNNNNNNNGC 7 cut(s) 11, 44, 585, 1028, 2249, 2530, 2658
BstNI CCWGG 5 cut(s) 121, 576, 804, 1281, 1835
BstNSI RCATGY 4 cut(s) 1165, 1210, 1355, 2560
BstPAI GACNNNNGTC 2 cut(s) 1122, 1551
BstPI GGTNACC 1 cut(s) 1801
BstSCI CCNGG 7 cut(s) 119, 574, 802, 1279, 1833, 2155, 2156
BstSFI CTRYAG 4 cut(s) 948, 1308, 1905, 1934
BstSLI GKGCMC 1 cut(s) 1796
BstSNI TACGTA 1 cut(s) 2402
BstUI CGCG 2 cut(s) 74, 327
BstV1I GCAGC 4 cut(s) 192, 291, 1162, 1294
BstV2I GAAGAC 2 cut(s) 1224, 2604
BstX2I RGATCY 4 cut(s) 763, 990, 1456, 1783
BstXI CCANNNNNNTGG 1 cut(s) 1074
BstYI RGATCY 4 cut(s) 763, 990, 1456, 1783
BsuRI GGCC 4 cut(s) 38, 495, 673, 2161
BtgZI GCGATG 1 cut(s) 62
BtsCI GGATG 4 cut(s) 144, 244, 2449, 2663
BtsIMutI CAGTG 3 cut(s) 2222, 2346, 2652
BtuMI TCGCGA 1 cut(s) 327
Cac8I GCNNGC 3 cut(s) 144, 1047, 1909
CaiI CAGNNNCTG 2 cut(s) 304, 2494
CfoI GCGC 4 cut(s) 319, 1831, 2199, 2299
Cfr13I GGNCC 5 cut(s) 82, 211, 572, 628, 2159
Cfr9I CCCGGG 1 cut(s) 2156
CseI GACGC 2 cut(s) 63, 1832
Csp6I GTAC 6 cut(s) 1198, 1349, 1563, 1658, 1708, 2102
CviQI GTAC 6 cut(s) 1198, 1349, 1563, 1658, 1708, 2102
DdeI CTNAG 7 cut(s) 131, 261, 297, 1599, 1626, 1718, 1780
DinI GGCGCC 1 cut(s) 2198
EaeI YGGCCR 1 cut(s) 36
Eam1104I CTCTTC 5 cut(s) 159, 440, 969, 1279, 2044
EarI CTCTTC 5 cut(s) 159, 440, 969, 1279, 2044
Eco105I TACGTA 1 cut(s) 2402
Eco130I CCWWGG 3 cut(s) 78, 1096, 1989
Eco147I AGGCCT 1 cut(s) 495
Eco31I GGTCTC 2 cut(s) 921, 1697
Eco47I GGWCC 4 cut(s) 82, 211, 572, 628
Eco57I CTGAAG 5 cut(s) 734, 1202, 1236, 2516, 2567
Eco88I CYCGRG 2 cut(s) 2156, 2456
Eco91I GGTNACC 1 cut(s) 1801
EcoO109I RGGNCCY 2 cut(s) 82, 572
EcoO65I GGTNACC 1 cut(s) 1801
EcoRI GAATTC 1 cut(s) 2567
EcoRII CCWGG 5 cut(s) 119, 574, 802, 1279, 1833
EcoT14I CCWWGG 3 cut(s) 78, 1096, 1989
EcoT22I ATGCAT 2 cut(s) 711, 1167
EgeI GGCGCC 1 cut(s) 2198
EheI GGCGCC 1 cut(s) 2198
ErhI CCWWGG 3 cut(s) 78, 1096, 1989
Esp3I CGTCTC 1 cut(s) 2186
FalI AAGNNNNNCTT 4 cut(s) 208, 240, 1965, 1997
FaqI GGGAC 1 cut(s) 170
FauI CCCGC 2 cut(s) 884, 2174
FauNDI CATATG 1 cut(s) 2509
FbaI TGATCA 1 cut(s) 2416
FblI GTMKAC 2 cut(s) 2028, 2209
Fnu4HI GCNGC 5 cut(s) 181, 305, 580, 1151, 1308
FokI GGATG 4 cut(s) 151, 251, 2456, 2670
Fsp4HI GCNGC 5 cut(s) 181, 305, 580, 1151, 1308
FspI TGCGCA 1 cut(s) 318
GlaI GCGC 4 cut(s) 318, 1830, 2198, 2298
GluI GCNGC 5 cut(s) 181, 305, 580, 1151, 1308
GsaI CCCAGC 1 cut(s) 2230
GsuI CTGGAG 3 cut(s) 1050, 1817, 1899
HaeII RGCGCY 2 cut(s) 2200, 2300
HaeIII GGCC 4 cut(s) 38, 495, 673, 2161
HapII CCGG 2 cut(s) 585, 2157
HgaI GACGC 2 cut(s) 63, 1832
HhaI GCGC 4 cut(s) 319, 1831, 2199, 2299
Hin1I GRCGYC 2 cut(s) 1501, 2197
Hin6I GCGC 4 cut(s) 317, 1829, 2197, 2297
HinP1I GCGC 4 cut(s) 317, 1829, 2197, 2297
HincII GTYRAC 2 cut(s) 2284, 2472
HindII GTYRAC 2 cut(s) 2284, 2472
HpaI GTTAAC 1 cut(s) 2472
HpaII CCGG 2 cut(s) 585, 2157
HphI GGTGA 2 cut(s) 1813, 1969
Hpy166II GTNNAC 7 cut(s) 962, 1658, 2029, 2210, 2284, 2382, 2472
Hpy8I GTNNAC 7 cut(s) 962, 1658, 2029, 2210, 2284, 2382, 2472
Hpy99I CGWCG 2 cut(s) 314, 1503
HpyAV CCTTC 9 cut(s) 37, 170, 403, 709, 925, 1001, 1259, 1605, 2581
HpyCH4III ACNGT 8 cut(s) 528, 598, 636, 1450, 1682, 2217, 2237, 2603
HpyCH4IV ACGT 4 cut(s) 312, 1501, 2179, 2401
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 44, 585, 1028, 2249, 2530, 2658
HpyF3I CTNAG 7 cut(s) 131, 261, 297, 1599, 1626, 1718, 1780
HpySE526I ACGT 4 cut(s) 312, 1501, 2179, 2401
Hsp92I GRCGYC 2 cut(s) 1501, 2197
HspAI GCGC 4 cut(s) 317, 1829, 2197, 2297
KasI GGCGCC 1 cut(s) 2196
Ksp22I TGATCA 1 cut(s) 2416
KspAI GTTAAC 1 cut(s) 2472
LmnI GCTCC 9 cut(s) 502, 587, 1360, 1386, 1836, 1919, 2304, 2447, 2649
Lsp1109I GCAGC 4 cut(s) 192, 291, 1162, 1294
LweI GCATC 6 cut(s) 129, 229, 696, 718, 1174, 2648
MaeII ACGT 4 cut(s) 312, 1501, 2179, 2401
MaeIII GTNAC 8 cut(s) 666, 1083, 1157, 1235, 1801, 1870, 2499, 2603
MflI RGATCY 4 cut(s) 763, 990, 1456, 1783
MhlI GDGCHC 2 cut(s) 1796, 2654
MluI ACGCGT 1 cut(s) 72
Mly113I GGCGCC 1 cut(s) 2197
MlyI GAGTC 5 cut(s) 152, 510, 869, 1112, 2634
MmeI TCCRAC 2 cut(s) 655, 2200
Mph1103I ATGCAT 2 cut(s) 711, 1167
MroXI GAANNNNTTC 1 cut(s) 2544
MslI CAYNNNNRTG 3 cut(s) 105, 599, 1799
MspA1I CMGCKG 1 cut(s) 1031
MspCI CTTAAG 2 cut(s) 1787, 2462
MspI CCGG 2 cut(s) 585, 2157
MspR9I CCNGG 7 cut(s) 121, 576, 804, 1281, 1835, 2157, 2158
Mva1269I GAATGC 1 cut(s) 2278
MvaI CCWGG 5 cut(s) 121, 576, 804, 1281, 1835
MvnI CGCG 2 cut(s) 74, 327
MwoI GCNNNNNNNGC 7 cut(s) 11, 44, 585, 1028, 2249, 2530, 2658
NarI GGCGCC 1 cut(s) 2197
NciI CCSGG 2 cut(s) 2157, 2158
NdeI CATATG 1 cut(s) 2509
NlaIV GGNNCC 6 cut(s) 84, 504, 583, 1278, 1458, 2198
NmeAIII GCCGAG 1 cut(s) 64
NmuCI GTSAC 4 cut(s) 666, 1235, 1801, 1870
NruI TCGCGA 1 cut(s) 327
NsbI TGCGCA 1 cut(s) 318
NsiI ATGCAT 2 cut(s) 711, 1167
NspI RCATGY 4 cut(s) 1165, 1210, 1355, 2560
OliI CACNNNNGTG 2 cut(s) 599, 1799
PceI AGGCCT 1 cut(s) 495
PctI GAATGC 1 cut(s) 2278
PdmI GAANNNNTTC 1 cut(s) 2544
PfeI GAWTC 9 cut(s) 265, 407, 542, 1246, 2221, 2354, 2491, 2545, 2617
PflMI CCANNNNNTGG 1 cut(s) 220
PfoI TCCNGGA 1 cut(s) 1833
PkrI GCNGC 5 cut(s) 182, 306, 581, 1152, 1309
PleI GAGTC 5 cut(s) 152, 510, 868, 1112, 2633
PluTI GGCGCC 1 cut(s) 2200
PpsI GAGTC 5 cut(s) 152, 510, 868, 1112, 2633
Ppu21I YACGTR 1 cut(s) 2402
PpuMI RGGWCCY 2 cut(s) 82, 572
PshAI GACNNNNGTC 2 cut(s) 1122, 1551
PshBI ATTAAT 1 cut(s) 1371
Psp5II RGGWCCY 2 cut(s) 82, 572
Psp6I CCWGG 5 cut(s) 119, 574, 802, 1279, 1833
PspEI GGTNACC 1 cut(s) 1801
PspFI CCCAGC 1 cut(s) 2226
PspGI CCWGG 5 cut(s) 119, 574, 802, 1279, 1833
PspN4I GGNNCC 6 cut(s) 84, 504, 583, 1278, 1458, 2198
PspPI GGNCC 5 cut(s) 82, 211, 572, 628, 2159
PspPPI RGGWCCY 2 cut(s) 82, 572
PstI CTGCAG 3 cut(s) 952, 1312, 1909
PstNI CAGNNNCTG 2 cut(s) 304, 2494
PsuI RGATCY 4 cut(s) 763, 990, 1456, 1783
PvuII CAGCTG 1 cut(s) 1031
RruI TCGCGA 1 cut(s) 327
RsaI GTAC 6 cut(s) 1199, 1350, 1564, 1659, 1709, 2103
RsaNI GTAC 6 cut(s) 1198, 1349, 1563, 1658, 1708, 2102
RseI CAYNNNNRTG 3 cut(s) 105, 599, 1799
SatI GCNGC 5 cut(s) 181, 305, 580, 1151, 1308
Sau96I GGNCC 5 cut(s) 82, 211, 572, 628, 2159
SchI GAGTC 5 cut(s) 152, 510, 869, 1112, 2634
ScrFI CCNGG 7 cut(s) 121, 576, 804, 1281, 1835, 2157, 2158
SduI GDGCHC 2 cut(s) 1796, 2654
SfaNI GCATC 6 cut(s) 129, 229, 696, 718, 1174, 2648
SfcI CTRYAG 4 cut(s) 948, 1308, 1905, 1934
SfoI GGCGCC 1 cut(s) 2198
SinI GGWCC 4 cut(s) 82, 211, 572, 628
SmaI CCCGGG 1 cut(s) 2158
SmiMI CAYNNNNRTG 3 cut(s) 105, 599, 1799
SmlI CTYRAG 7 cut(s) 416, 473, 497, 551, 1702, 1787, 2462
SmoI CTYRAG 7 cut(s) 416, 473, 497, 551, 1702, 1787, 2462
SnaBI TACGTA 1 cut(s) 2402
SpeI ACTAGT 1 cut(s) 1570
SseBI AGGCCT 1 cut(s) 495
SsiI CCGC 3 cut(s) 579, 891, 2167
SspDI GGCGCC 1 cut(s) 2196
SspI AATATT 1 cut(s) 1915
StuI AGGCCT 1 cut(s) 495
StyD4I CCNGG 7 cut(s) 119, 574, 802, 1279, 1833, 2155, 2156
StyI CCWWGG 3 cut(s) 78, 1096, 1989
TaaI ACNGT 8 cut(s) 528, 598, 636, 1450, 1682, 2217, 2237, 2603
TaiI ACGT 4 cut(s) 315, 1504, 2182, 2404
TaqI TCGA 7 cut(s) 660, 1130, 1378, 1411, 1498, 2439, 2623
TatI WGTACW 1 cut(s) 1197
TauI GCSGC 1 cut(s) 582
TfiI GAWTC 9 cut(s) 265, 407, 542, 1246, 2221, 2354, 2491, 2545, 2617
TscAI CASTG 3 cut(s) 2222, 2353, 2659
TseFI GTSAC 4 cut(s) 666, 1235, 1801, 1870
TseI GCWGC 4 cut(s) 180, 304, 1150, 1307
Tsp45I GTSAC 4 cut(s) 666, 1235, 1801, 1870
TspGWI ACGGA 1 cut(s) 2000
TspMI CCCGGG 1 cut(s) 2156
TspRI CASTG 3 cut(s) 2222, 2353, 2659
Van91I CCANNNNNTGG 1 cut(s) 220
Vha464I CTTAAG 2 cut(s) 1787, 2462
VpaK11BI GGWCC 4 cut(s) 82, 211, 572, 628
VspI ATTAAT 1 cut(s) 1371
XapI RAATTY 3 cut(s) 1342, 1494, 2567
XbaI TCTAGA 1 cut(s) 2480
XceI RCATGY 4 cut(s) 1165, 1210, 1355, 2560
XcmI CCANNNNNNNNNTGG 1 cut(s) 529
XmaI CCCGGG 1 cut(s) 2156
XmiI GTMKAC 2 cut(s) 2028, 2209
XmnI GAANNNNTTC 1 cut(s) 2544
ZraI GACGTC 1 cut(s) 1502
Zsp2I ATGCAT 2 cut(s) 711, 1167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.