Rh2CG059900

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000865
Physical Location & Seq
Reverse (-)
4871402 .. 4872403
1002 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG059900.1

Sequence Viewer

Length: 588 bp
ATGCTGTCACTGATTAAGGTGCCAAACACAGATTTCATTAAATCCTTGAGAAAACTGAGACTTCTCAGGAAGTTGGGGCTGAAAGACCTTCAAACAACAGATGGTAAAGAATTGTGCAGTGTCCTTGAGCAAATGAAGGGTCTCTTAACCTTGAATGCAAGAGTGAAAGACAAGGAGTGTCTCGATTTAGATCATTGTGCCAGCCCTCCTCGATTTCTCCGGCATCTATGTTTGAGAGGCCCCCTAGCAGGGATACCAAGCTGGATCTCCAAGCTTAGTAGTCTGGAAAAGGTTGGCTTTTATTGGTCAAACCTAAGTTCTAACCCGCTTCAAATTCTTCAAGATTTGCCCTATCTTTTGGAGCTCAAAATGGTGCAGTATTACCAAGGGGAAAAGTTGGTATTTGAAGCTGGAAAATTCAAGAACAGTACACTGATCTTAGAAGAATTTGATCAGCTTAAATCATTGGAGGCTGAGGATGGGGCATTGCATGAACTTAGGGAATTGAAAGTGTCTAGATGTCCTAATCTAAAGTCTGTTCCAAAAGAGATTAGATCTCTCACAACTTTTTGCTTGTTTTGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

195

Amino Acids

22.43

Weight (kDa)

8.99

Isoelectric Point (pI)

40.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 2 - 172 6e-16 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 19
AciI CCGC 1 cut(s) 326
AclWI GGATC 1 cut(s) 272
AcsI RAATTY 3 cut(s) 333, 416, 446
AfaI GTAC 1 cut(s) 430
AfiI CCNNNNNNNGG 2 cut(s) 248, 249
AgsI TTSAA 7 cut(s) 92, 154, 332, 341, 407, 421, 508
AluBI AGCT 5 cut(s) 261, 274, 364, 410, 457
AluI AGCT 5 cut(s) 261, 274, 364, 410, 457
Alw21I GWGCWC 1 cut(s) 366
Alw26I GTCTC 3 cut(s) 52, 146, 185
AlwI GGATC 1 cut(s) 272
AoxI GGCC 1 cut(s) 238
ApoI RAATTY 3 cut(s) 333, 416, 446
ArsI GACNNNNNNTTYG 2 cut(s) 124, 156
AspS9I GGNCC 1 cut(s) 239
BanI GGYRCC 1 cut(s) 19
BanII GRGCYC 1 cut(s) 366
Bbv12I GWGCWC 1 cut(s) 366
BbvCI CCTCAGC 1 cut(s) 474
BccI CCATC 2 cut(s) 95, 473
BciVI GTATCC 1 cut(s) 246
BclI TGATCA 1 cut(s) 451
BcoDI GTCTC 3 cut(s) 52, 146, 185
BfaI CTAG 2 cut(s) 245, 516
BfuI GTATCC 1 cut(s) 246
BglII AGATCT 1 cut(s) 554
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 2 cut(s) 21, 241
BmsI GCATC 1 cut(s) 232
Bpu10I CCTNAGC 1 cut(s) 474
BpuEI CTTGAG 2 cut(s) 67, 146
BsaBI GATNNNNATC 1 cut(s) 189
BsaI GGTCTC 1 cut(s) 146
BsaJI CCNNGG 1 cut(s) 385
Bsc4I CCNNNNNNNGG 2 cut(s) 248, 249
Bse3DI GCAATG 1 cut(s) 485
Bse8I GATNNNNATC 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 385
BseGI GGATG 1 cut(s) 484
BseJI GATNNNNATC 1 cut(s) 189
BseLI CCNNNNNNNGG 2 cut(s) 248, 249
BseMI GCAATG 1 cut(s) 485
BseMII CTCAG 3 cut(s) 47, 79, 465
BseRI GAGGAG 1 cut(s) 198
BsgI GTGCAG 2 cut(s) 136, 395
BshFI GGCC 1 cut(s) 240
BshNI GGYRCC 1 cut(s) 19
BsiHKAI GWGCWC 1 cut(s) 366
BsiSI CCGG 1 cut(s) 220
BslI CCNNNNNNNGG 2 cut(s) 248, 249
BsmAI GTCTC 3 cut(s) 52, 146, 185
BsmI GAATGC 1 cut(s) 160
BsnI GGCC 1 cut(s) 240
Bso31I GGTCTC 1 cut(s) 146
Bsp1286I GDGCHC 1 cut(s) 366
Bsp143I GATC 5 cut(s) 190, 264, 435, 451, 554
BspACI CCGC 1 cut(s) 326
BspANI GGCC 1 cut(s) 240
BspCNI CTCAG 3 cut(s) 48, 78, 466
BspLI GGNNCC 2 cut(s) 21, 241
BspPI GGATC 1 cut(s) 272
BspT107I GGYRCC 1 cut(s) 19
BspTNI GGTCTC 1 cut(s) 146
BsrDI GCAATG 1 cut(s) 485
BssECI CCNNGG 1 cut(s) 385
BssMI GATC 5 cut(s) 190, 264, 435, 451, 554
BssT1I CCWWGG 1 cut(s) 385
Bst4CI ACNGT 1 cut(s) 428
BstC8I GCNNGC 1 cut(s) 202
BstDEI CTNAG 7 cut(s) 56, 65, 275, 314, 439, 474, 497
BstF5I GGATG 1 cut(s) 484
BstKTI GATC 5 cut(s) 193, 267, 438, 454, 557
BstMAI GTCTC 3 cut(s) 52, 146, 185
BstMBI GATC 5 cut(s) 190, 264, 435, 451, 554
BstX2I RGATCY 2 cut(s) 264, 554
BstYI RGATCY 2 cut(s) 264, 554
BsuI GTATCC 1 cut(s) 246
BsuRI GGCC 1 cut(s) 240
BtsCI GGATG 1 cut(s) 484
BtsI GCAGTG 1 cut(s) 124
BtsIMutI CAGTG 3 cut(s) 8, 124, 431
Cac8I GCNNGC 1 cut(s) 202
Cfr13I GGNCC 1 cut(s) 239
Csp6I GTAC 1 cut(s) 429
CviAII CATG 1 cut(s) 491
CviQI GTAC 1 cut(s) 429
DdeI CTNAG 7 cut(s) 56, 65, 275, 314, 439, 474, 497
DpnI GATC 5 cut(s) 192, 266, 437, 453, 556
DpnII GATC 5 cut(s) 190, 264, 435, 451, 554
Ecl136II GAGCTC 1 cut(s) 364
Eco130I CCWWGG 1 cut(s) 385
Eco24I GRGCYC 1 cut(s) 366
Eco31I GGTCTC 1 cut(s) 146
Eco53kI GAGCTC 1 cut(s) 364
EcoICRI GAGCTC 1 cut(s) 364
EcoO109I RGGNCCY 1 cut(s) 239
EcoT14I CCWWGG 1 cut(s) 385
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 1 cut(s) 385
FaeI CATG 1 cut(s) 494
FaiI YATR 2 cut(s) 229, 492
FalI AAGNNNNNCTT 4 cut(s) 128, 160, 281, 313
FatI CATG 1 cut(s) 490
FauI CCCGC 1 cut(s) 333
FbaI TGATCA 1 cut(s) 451
FokI GGATG 1 cut(s) 491
FriOI GRGCYC 1 cut(s) 366
FspBI CTAG 2 cut(s) 245, 516
HaeIII GGCC 1 cut(s) 240
HapII CCGG 1 cut(s) 220
Hin1II CATG 1 cut(s) 494
HindIII AAGCTT 1 cut(s) 272
HpaII CCGG 1 cut(s) 220
Hpy166II GTNNAC 1 cut(s) 431
Hpy188III TCNNGA 6 cut(s) 67, 182, 284, 341, 421, 516
Hpy8I GTNNAC 1 cut(s) 431
HpyAV CCTTC 2 cut(s) 98, 130
HpyCH4III ACNGT 1 cut(s) 428
HpyCH4V TGCA 4 cut(s) 117, 158, 376, 490
HpyF3I CTNAG 7 cut(s) 56, 65, 275, 314, 439, 474, 497
Hsp92II CATG 1 cut(s) 494
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 5 cut(s) 190, 264, 435, 451, 554
LmnI GCTCC 1 cut(s) 361
LpnPI CCDG 7 cut(s) 52, 214, 233, 234, 247, 269, 396
LweI GCATC 1 cut(s) 232
MaeI CTAG 2 cut(s) 245, 516
MaeIII GTNAC 1 cut(s) 6
MalI GATC 5 cut(s) 192, 266, 437, 453, 556
MboI GATC 5 cut(s) 190, 264, 435, 451, 554
MboII GAAGA 2 cut(s) 329, 455
MflI RGATCY 2 cut(s) 264, 554
MhlI GDGCHC 1 cut(s) 366
MluCI AATT 5 cut(s) 110, 333, 416, 446, 503
MnlI CCTC 5 cut(s) 216, 219, 230, 463, 469
MseI TTAA 4 cut(s) 15, 39, 146, 459
MspI CCGG 1 cut(s) 220
Mva1269I GAATGC 1 cut(s) 160
NdeII GATC 5 cut(s) 190, 264, 435, 451, 554
NlaIII CATG 1 cut(s) 494
NlaIV GGNNCC 2 cut(s) 21, 241
NmuCI GTSAC 1 cut(s) 6
PctI GAATGC 1 cut(s) 160
Psp124BI GAGCTC 1 cut(s) 366
PspN4I GGNNCC 2 cut(s) 21, 241
PspPI GGNCC 1 cut(s) 239
PsuI RGATCY 2 cut(s) 264, 554
RsaI GTAC 1 cut(s) 430
RsaNI GTAC 1 cut(s) 429
SacI GAGCTC 1 cut(s) 366
SaqAI TTAA 4 cut(s) 15, 39, 146, 459
Sau3AI GATC 5 cut(s) 190, 264, 435, 451, 554
Sau96I GGNCC 1 cut(s) 239
SduI GDGCHC 1 cut(s) 366
SfaNI GCATC 1 cut(s) 232
SmlI CTYRAG 2 cut(s) 46, 125
SmoI CTYRAG 2 cut(s) 46, 125
Sse9I AATT 5 cut(s) 110, 333, 416, 446, 503
SsiI CCGC 1 cut(s) 326
SspMI CTAG 2 cut(s) 245, 516
SstI GAGCTC 1 cut(s) 366
StyI CCWWGG 1 cut(s) 385
TaaI ACNGT 1 cut(s) 428
TaqI TCGA 2 cut(s) 183, 211
TasI AATT 5 cut(s) 110, 333, 416, 446, 503
TatI WGTACW 1 cut(s) 428
Tru1I TTAA 4 cut(s) 15, 39, 146, 459
Tru9I TTAA 4 cut(s) 15, 39, 146, 459
TscAI CASTG 3 cut(s) 15, 124, 438
TseFI GTSAC 1 cut(s) 6
Tsp45I GTSAC 1 cut(s) 6
TspDTI ATGAA 3 cut(s) 25, 149, 507
TspRI CASTG 3 cut(s) 15, 124, 438
XapI RAATTY 3 cut(s) 333, 416, 446
XbaI TCTAGA 1 cut(s) 515
XspI CTAG 2 cut(s) 245, 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.