Rmu_sc0004851.1_g000010

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004851.1
OG0000865
Physical Location & Seq
Forward (+)
38794 .. 41472
2679 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004851.1_g000010.1.cds

Sequence Viewer

Length: 2679 bp
atggctgaattttccgttcaagaagcattagccctagcttggagggtgagaagcctcctgtgctataagttcgaacgtccaacttatctacataaagatatagatagagtcgagagagttttgaatgtgatgaatgcctatctaatcgacttcgaaggtggcatttatgaaaacagacaccaaatccaagagatagctactgatatcgtggaacttcttgacaaattgatgcataagctggtttcacacgatcatggtgtagacaagttgatgcataagcaggttttgaaattgatgaaacaagtcgcattttgggtcacaagtctcaatttgtccgcaagaattgatagaattgatgtgaaaaacactgattttgtcagaaaactagcttcacagccgtacgactcgcgacgcgtctcgaaatcgagactaggcggctgttcaccacaggatagtgaacttgcaaactataacgaagtacagggctttgaagcgcttgcacaaaaactcttcgaaaatttgatagatgaagaaccacagctttcaacaatttgtgttgtcggtcctggtggatctggaaaatccaccctggtaaattttgttttcaagtggaaggttgtagagttgtttgagtgcaagacttgggttcgtgtgacgcgtcggccacttccactcaaggaactcctgcaacacatgctgactgattttgggtataaaaattcagaagaaatcgataatccaggagcaaaattgagactttttctgcagcaaaggaggtatctggttgtcttagacgatgtttggagtacacaagatttcgattgcattgtaaaagcgttaccaaaaggtgctcccggaagtaagatggtaataactagtcgtaactctagtgtcgtctcttttctctcctccaagtatatccatgatttgagcagtgggttgtcacctgaagcggccaagaatctattcaccaggaaggttctccggaatcatctaagaaatttgcaacccgaggtggaggagtgcgctgataaaattcttcaatggtgcgggaaccagccctttgcaatttcagcggtcggtagtttgctagcagagaagccacaaactcgagcagagtgggaggatatttataacaatcttggatctaaaactggaccgggctctattcttgaaattgttagcaaaattttgcagccgagttttatggatcttccgaaccatcttaaatcctgttttctgtacttgagcatttttcctcccaactatgccatccagcctgagaggttaattcacttatgggttgcggaaggacttgcggaacgttatgcctcctccagtaagatgccgaaggagtatgttgcacaaagttatttgaatgaacttatctctagaaatctggttcttgccactgaaagagaaatcaatggacaactacagagttgttgtgttgttttgcaccatgtccgtgattacatcctttcaaaagaggagaatttcgttactgttttggagtctgacgcggaccggaggactaggcctgcatttgagaaaattcgacgcctttcagtgttaaaagctgattgtgtcagtctgcggtactctttcaacttgaatcatttgtataccttagtggtctttggtagttggtcttattctgagcttgaatgggtacttgaaaactgtaaatatttgagggttttagatttgcaaggagtaactaatttgaaggagtttcccgaaaacttttccattaagggtctcattctcttgaagtacttgagtctgcggggatcaaggaatacaacagttccaaagtccataagaaagtatcaatgcttggagaccttagttcttgaagacaccatggtgatcaaattgccagaaaaagagatgcgtgtactcaagaatctgcgccatttttcagtctcgtccaatcatactggcggagtgatgttatcggctggcaatattaaagctttatcttccatacacgggttgtcactgattaaggtgaaaaacaacagagcaatcataaaagccttgggagagttgaaaaatgttaggaatttaggactggtagatcttgaaacacaagatggaaaagaactatgtactgcccttgagaaaatgaagcatctttcgacacttgatataagagcagaacgcctggagttccttgatttggatcatatgcgatcccctcctcattttctccaaggtctacatttggaaggacgcctagaagggctacctaagtggattctccagcttagtagtctagagaagattggtttacatggttcagagctgagggctaacgttaacccacttgagctccttgaagctctgccttatctgcttgagcttgacttggtggcctattacaaaggggaaaagttgatattcacagctggtacattcaagcgtctaatgatattaaaaatggaacaatttactgagctgaattccatttcatttgacgacggagcaatgccgcggcttagggagctaaccatctctagatgtccgaatctgagttctcttccaaacggcattcgtggtcttagttcactctacaagctggatttggattacatgccccataaacttattcaattgtccgcaccgtcagatggttcagcatattcccgactccaattttacaactgtttctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

892

Amino Acids

101.81

Weight (kDa)

8.61

Isoelectric Point (pI)

42.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1134
Acc36I ACCTGC 1 cut(s) 271
AccI GTMKAC 3 cut(s) 261, 1625, 2224
AccII CGCG 5 cut(s) 409, 414, 658, 1523, 2500
AccIII TCCGGA 1 cut(s) 984
AclI AACGTT 2 cut(s) 1324, 2322
AclWI GGATC 6 cut(s) 580, 1153, 1218, 1801, 2193, 2196
AcoI YGGCCR 2 cut(s) 662, 954
AcuI CTGAAG 1 cut(s) 969
AcyI GRCGYC 2 cut(s) 1561, 2239
AfeI AGCGCT 1 cut(s) 495
AfiI CCNNNNNNNGG 4 cut(s) 39, 1995, 2185, 2636
AflIII ACRYGT 2 cut(s) 412, 656
AhlI ACTAGT 1 cut(s) 875
AjnI CCWGG 5 cut(s) 565, 588, 739, 971, 2169
AjuI GAANNNNNNNTTGG 2 cut(s) 950, 982
AleI CACNNNNGTG 1 cut(s) 1868
Alw21I GWGCWC 2 cut(s) 853, 2340
Alw26I GTCTC 8 cut(s) 329, 421, 421, 748, 901, 1766, 1838, 1933
AlwI GGATC 6 cut(s) 580, 1153, 1218, 1801, 2193, 2196
Ama87I CYCGRG 2 cut(s) 1010, 1110
Aor13HI TCCGGA 1 cut(s) 984
Aor51HI AGCGCT 1 cut(s) 495
AoxI GGCC 4 cut(s) 662, 954, 1538, 2379
ApeKI GCWGC 2 cut(s) 766, 1195
Asp700I GAANNNNTTC 1 cut(s) 965
AspLEI GCGC 3 cut(s) 496, 1028, 1917
AspS9I GGNCC 3 cut(s) 563, 1157, 1525
AsuC2I CCSGG 2 cut(s) 855, 1161
AsuHPI GGTGA 6 cut(s) 58, 435, 936, 961, 1882, 2026
AsuII TTCGAA 3 cut(s) 72, 153, 513
AsuNHI GCTAGC 1 cut(s) 1090
AvaI CYCGRG 2 cut(s) 1010, 1110
AvaII GGWCC 3 cut(s) 563, 1157, 1525
BanII GRGCYC 2 cut(s) 1166, 2340
BbsI GAAGAC 1 cut(s) 1866
Bbv12I GWGCWC 2 cut(s) 853, 2340
BbvCI CCTCAGC 1 cut(s) 2312
BbvI GCAGC 2 cut(s) 778, 1207
BccI CCATC 6 cut(s) 859, 1230, 1280, 2093, 2525, 2630
BceAI ACGGC 2 cut(s) 382, 2569
BciT130I CCWGG 5 cut(s) 567, 590, 741, 973, 2171
BclI TGATCA 1 cut(s) 1872
BcnI CCSGG 2 cut(s) 855, 1161
BcoDI GTCTC 8 cut(s) 329, 421, 421, 748, 901, 1766, 1838, 1933
BcuI ACTAGT 1 cut(s) 875
BfmI CTRYAG 2 cut(s) 764, 1436
BfoI RGCGCY 1 cut(s) 497
BfuAI ACCTGC 1 cut(s) 271
BglII AGATCT 1 cut(s) 2083
BisI GCNGC 6 cut(s) 436, 767, 954, 1196, 2498, 2501
BlsI GCNGC 6 cut(s) 437, 768, 955, 1197, 2499, 2502
BmcAI AGTACT 1 cut(s) 1778
Bme1390I CCNGG 7 cut(s) 567, 590, 741, 855, 973, 1161, 2171
Bme18I GGWCC 3 cut(s) 563, 1157, 1525
BmeT110I CYCGRG 2 cut(s) 1010, 1110
BmgT120I GGNCC 3 cut(s) 563, 1157, 1525
BmiI GGNNCC 1 cut(s) 1055
BmrFI CCNGG 7 cut(s) 567, 590, 741, 855, 973, 1161, 2171
BmsI GCATC 5 cut(s) 219, 261, 1335, 1884, 2146
BmtI GCTAGC 1 cut(s) 1094
BpiI GAAGAC 1 cut(s) 1866
BpmI CTGGAG 3 cut(s) 1321, 2192, 2252
Bpu10I CCTNAGC 2 cut(s) 2312, 2504
Bpu14I TTCGAA 3 cut(s) 72, 153, 513
BpuEI CTTGAG 7 cut(s) 659, 1267, 1801, 1889, 2144, 2354, 2384
BpuMI CCSGG 2 cut(s) 855, 1161
Bsa29I ATCGAT 1 cut(s) 732
BsaBI GATNNNNATC 1 cut(s) 2187
BsaHI GRCGYC 2 cut(s) 1561, 2239
BsaI GGTCTC 2 cut(s) 1766, 1838
BsaJI CCNNGG 6 cut(s) 588, 1011, 1866, 2043, 2218, 2498
BsaWI WCCGGW 2 cut(s) 984, 1527
BsaXI ACNNNNNCTCC 2 cut(s) 2322, 2352
Bsc4I CCNNNNNNNGG 4 cut(s) 39, 1995, 2185, 2636
Bse1I ACTGG 4 cut(s) 1159, 1338, 1948, 2082
Bse3DI GCAATG 1 cut(s) 2499
Bse8I GATNNNNATC 1 cut(s) 2187
BseAI TCCGGA 1 cut(s) 984
BseBI CCWGG 5 cut(s) 567, 590, 741, 973, 2171
BseCI ATCGAT 1 cut(s) 732
BseDI CCNNGG 6 cut(s) 588, 1011, 1866, 2043, 2218, 2498
BseGI GGATG 2 cut(s) 1272, 1476
BseJI GATNNNNATC 1 cut(s) 2187
BseLI CCNNNNNNNGG 4 cut(s) 39, 1995, 2185, 2636
BseMI GCAATG 1 cut(s) 2499
BseMII CTCAG 5 cut(s) 1272, 1650, 2303, 2451, 2528
BseNI ACTGG 4 cut(s) 1159, 1338, 1948, 2082
BseRI GAGGAG 5 cut(s) 898, 1034, 1324, 1505, 2196
BseXI GCAGC 2 cut(s) 778, 1207
Bsh1236I CGCG 5 cut(s) 409, 414, 658, 1523, 2500
Bsh1285I CGRYCG 1 cut(s) 1080
BshFI GGCC 4 cut(s) 664, 956, 1540, 2381
BshVI ATCGAT 1 cut(s) 732
BsiEI CGRYCG 1 cut(s) 1080
BsiHKAI GWGCWC 2 cut(s) 853, 2340
BsiHKCI CYCGRG 2 cut(s) 1010, 1110
BsiSI CCGG 4 cut(s) 855, 985, 1160, 1528
BsiWI CGTACG 1 cut(s) 399
BslI CCNNNNNNNGG 4 cut(s) 39, 1995, 2185, 2636
BsmAI GTCTC 8 cut(s) 329, 421, 421, 748, 901, 1766, 1838, 1933
BsmBI CGTCTC 2 cut(s) 421, 901
BsmI GAATGC 2 cut(s) 139, 2556
BsnI GGCC 4 cut(s) 664, 956, 1540, 2381
Bso31I GGTCTC 2 cut(s) 1766, 1838
BsoBI CYCGRG 2 cut(s) 1010, 1110
Bsp119I TTCGAA 3 cut(s) 72, 153, 513
Bsp1286I GDGCHC 3 cut(s) 853, 1166, 2340
Bsp13I TCCGGA 1 cut(s) 984
Bsp143I GATC 9 cut(s) 250, 572, 1145, 1210, 1793, 1872, 2083, 2188, 2198
Bsp19I CCATGG 1 cut(s) 1866
Bsp68I TCGCGA 1 cut(s) 409
BspANI GGCC 4 cut(s) 664, 956, 1540, 2381
BspCNI CTCAG 5 cut(s) 1273, 1651, 2304, 2452, 2529
BspDI ATCGAT 1 cut(s) 732
BspEI TCCGGA 1 cut(s) 984
BspFNI CGCG 5 cut(s) 409, 414, 658, 1523, 2500
BspLI GGNNCC 1 cut(s) 1055
BspMAI CTGCAG 1 cut(s) 768
BspMI ACCTGC 1 cut(s) 271
BspOI GCTAGC 1 cut(s) 1094
BspPI GGATC 6 cut(s) 580, 1153, 1218, 1801, 2193, 2196
BspT104I TTCGAA 3 cut(s) 72, 153, 513
BspTNI GGTCTC 2 cut(s) 1766, 1838
BsrDI GCAATG 1 cut(s) 2499
BsrI ACTGG 4 cut(s) 1159, 1338, 1948, 2082
BssECI CCNNGG 6 cut(s) 588, 1011, 1866, 2043, 2218, 2498
BssMI GATC 9 cut(s) 250, 572, 1145, 1210, 1793, 1872, 2083, 2188, 2198
BssNAI GTATAC 1 cut(s) 1626
BssNI GRCGYC 2 cut(s) 1561, 2239
BssT1I CCWWGG 3 cut(s) 1866, 2043, 2218
Bst1107I GTATAC 1 cut(s) 1626
Bst2UI CCWGG 5 cut(s) 567, 590, 741, 973, 2171
Bst4CI ACNGT 5 cut(s) 1507, 1685, 1810, 2631, 2672
Bst6I CTCTTC 2 cut(s) 515, 2550
BstACI GRCGYC 2 cut(s) 1561, 2239
BstAPI GCANNNNNTGC 1 cut(s) 694
BstBI TTCGAA 3 cut(s) 72, 153, 513
BstC8I GCNNGC 4 cut(s) 498, 1092, 1542, 1966
BstDSI CCRYGG 2 cut(s) 1866, 2498
BstF5I GGATG 2 cut(s) 1272, 1476
BstFNI CGCG 5 cut(s) 409, 414, 658, 1523, 2500
BstH2I RGCGCY 1 cut(s) 497
BstHHI GCGC 3 cut(s) 496, 1028, 1917
BstKTI GATC 9 cut(s) 253, 575, 1148, 1213, 1796, 1875, 2086, 2191, 2201
BstMAI GTCTC 8 cut(s) 329, 421, 421, 748, 901, 1766, 1838, 1933
BstMBI GATC 9 cut(s) 250, 572, 1145, 1210, 1793, 1872, 2083, 2188, 2198
BstMCI CGRYCG 1 cut(s) 1080
BstMWI GCNNNNNNNGC 5 cut(s) 60, 694, 1073, 2359, 2509
BstNI CCWGG 5 cut(s) 567, 590, 741, 973, 2171
BstNSI RCATGY 2 cut(s) 697, 2602
BstSCI CCNGG 7 cut(s) 565, 588, 739, 853, 971, 1159, 2169
BstSFI CTRYAG 2 cut(s) 764, 1436
BstUI CGCG 5 cut(s) 409, 414, 658, 1523, 2500
BstV1I GCAGC 2 cut(s) 778, 1207
BstV2I GAAGAC 1 cut(s) 1866
BstX2I RGATCY 4 cut(s) 572, 1145, 1210, 2083
BstYI RGATCY 4 cut(s) 572, 1145, 1210, 2083
BstZ17I GTATAC 1 cut(s) 1626
Bsu15I ATCGAT 1 cut(s) 732
BsuRI GGCC 4 cut(s) 664, 956, 1540, 2381
BsuTUI ATCGAT 1 cut(s) 732
BtgI CCRYGG 2 cut(s) 1866, 2498
BtsCI GGATG 2 cut(s) 1272, 1476
BtsI GCAGTG 1 cut(s) 940
BtsIMutI CAGTG 5 cut(s) 366, 940, 1410, 1575, 2003
BtuMI TCGCGA 1 cut(s) 409
BveI ACCTGC 1 cut(s) 271
Cac8I GCNNGC 4 cut(s) 498, 1092, 1542, 1966
CfoI GCGC 3 cut(s) 496, 1028, 1917
Cfr13I GGNCC 3 cut(s) 563, 1157, 1525
Cfr42I CCGCGG 1 cut(s) 2501
ClaI ATCGAT 1 cut(s) 732
CpoI CGGWCCG 1 cut(s) 1525
CseI GACGC 8 cut(s) 403, 420, 647, 664, 1529, 1569, 2247, 2417
CspI CGGWCCG 1 cut(s) 1525
CviAII CATG 7 cut(s) 254, 694, 923, 1463, 1867, 2300, 2599
DpnI GATC 9 cut(s) 252, 574, 1147, 1212, 1795, 1874, 2085, 2190, 2200
DpnII GATC 9 cut(s) 250, 572, 1145, 1210, 1793, 1872, 2083, 2188, 2198
EaeI YGGCCR 2 cut(s) 662, 954
Eam1104I CTCTTC 2 cut(s) 515, 2550
EarI CTCTTC 2 cut(s) 515, 2550
EciI GGCGGA 1 cut(s) 1962
Ecl136II GAGCTC 1 cut(s) 2338
Eco130I CCWWGG 3 cut(s) 1866, 2043, 2218
Eco147I AGGCCT 1 cut(s) 1540
Eco24I GRGCYC 2 cut(s) 1166, 2340
Eco31I GGTCTC 2 cut(s) 1766, 1838
Eco32I GATATC 1 cut(s) 205
Eco47I GGWCC 3 cut(s) 563, 1157, 1525
Eco47III AGCGCT 1 cut(s) 495
Eco53kI GAGCTC 1 cut(s) 2338
Eco57I CTGAAG 1 cut(s) 969
Eco88I CYCGRG 2 cut(s) 1010, 1110
EcoICRI GAGCTC 1 cut(s) 2338
EcoRI GAATTC 1 cut(s) 2467
EcoRII CCWGG 5 cut(s) 565, 588, 739, 971, 2169
EcoRV GATATC 1 cut(s) 205
EcoT14I CCWWGG 3 cut(s) 1866, 2043, 2218
EcoT22I ATGCAT 2 cut(s) 234, 276
EcoT38I GRGCYC 2 cut(s) 1166, 2340
ErhI CCWWGG 3 cut(s) 1866, 2043, 2218
Esp3I CGTCTC 2 cut(s) 421, 901
FaeI CATG 7 cut(s) 257, 697, 926, 1466, 1870, 2303, 2602
FatI CATG 7 cut(s) 253, 693, 922, 1462, 1866, 2299, 2598
FauI CCCGC 2 cut(s) 1043, 1782
FauNDI CATATG 1 cut(s) 2193
FbaI TGATCA 1 cut(s) 1872
FblI GTMKAC 3 cut(s) 261, 1625, 2224
Fnu4HI GCNGC 6 cut(s) 436, 767, 954, 1196, 2498, 2501
FokI GGATG 2 cut(s) 1259, 1463
FriOI GRGCYC 2 cut(s) 1166, 2340
Fsp4HI GCNGC 6 cut(s) 436, 767, 954, 1196, 2498, 2501
GlaI GCGC 3 cut(s) 495, 1027, 1916
GluI GCNGC 6 cut(s) 436, 767, 954, 1196, 2498, 2501
GsuI CTGGAG 3 cut(s) 1321, 2192, 2252
HaeII RGCGCY 1 cut(s) 497
HaeIII GGCC 4 cut(s) 664, 956, 1540, 2381
HapII CCGG 4 cut(s) 855, 985, 1160, 1528
HgaI GACGC 8 cut(s) 403, 420, 647, 664, 1529, 1569, 2247, 2417
HhaI GCGC 3 cut(s) 496, 1028, 1917
Hin1I GRCGYC 2 cut(s) 1561, 2239
Hin1II CATG 7 cut(s) 257, 697, 926, 1466, 1870, 2303, 2602
Hin6I GCGC 3 cut(s) 494, 1026, 1915
HinP1I GCGC 3 cut(s) 494, 1026, 1915
HincII GTYRAC 1 cut(s) 2326
HindII GTYRAC 1 cut(s) 2326
HindIII AAGCTT 1 cut(s) 1977
HpaI GTTAAC 1 cut(s) 2326
HpaII CCGG 4 cut(s) 855, 985, 1160, 1528
HphI GGTGA 6 cut(s) 58, 435, 936, 961, 1882, 2026
Hpy188I TCNGA 9 cut(s) 380, 724, 1218, 1519, 1660, 2308, 2532, 2538, 2635
Hpy99I CGWCG 4 cut(s) 414, 663, 1563, 2489
HpyAV CCTTC 8 cut(s) 149, 607, 970, 1304, 1345, 1723, 2228, 2240
HpyCH4III ACNGT 5 cut(s) 1507, 1685, 1810, 2631, 2672
HpyCH4IV ACGT 3 cut(s) 76, 1324, 2322
HpyF10VI GCNNNNNNNGC 5 cut(s) 60, 694, 1073, 2359, 2509
HpySE526I ACGT 3 cut(s) 76, 1324, 2322
Hsp92I GRCGYC 2 cut(s) 1561, 2239
Hsp92II CATG 7 cut(s) 257, 697, 926, 1466, 1870, 2303, 2602
HspAI GCGC 3 cut(s) 494, 1026, 1915
Kpn2I TCCGGA 1 cut(s) 984
Ksp22I TGATCA 1 cut(s) 1872
KspAI GTTAAC 1 cut(s) 2326
KspI CCGCGG 1 cut(s) 2501
Kzo9I GATC 9 cut(s) 250, 572, 1145, 1210, 1793, 1872, 2083, 2188, 2198
LmnI GCTCC 5 cut(s) 743, 856, 2343, 2489, 2509
Lsp1109I GCAGC 2 cut(s) 778, 1207
LweI GCATC 5 cut(s) 219, 261, 1335, 1884, 2146
MaeII ACGT 3 cut(s) 76, 1324, 2322
MaeIII GTNAC 8 cut(s) 316, 652, 837, 881, 942, 1501, 1717, 2001
MalI GATC 9 cut(s) 252, 574, 1147, 1212, 1795, 1874, 2085, 2190, 2200
MboI GATC 9 cut(s) 250, 572, 1145, 1210, 1793, 1872, 2083, 2188, 2198
MboII GAAGA 9 cut(s) 502, 542, 737, 1031, 1205, 1871, 1977, 2299, 2537
MfeI CAATTG 1 cut(s) 2618
MflI RGATCY 4 cut(s) 572, 1145, 1210, 2083
MhlI GDGCHC 3 cut(s) 853, 1166, 2340
MluI ACGCGT 2 cut(s) 412, 656
MlyI GAGTC 5 cut(s) 117, 398, 1523, 1792, 2649
MmeI TCCRAC 1 cut(s) 104
Mph1103I ATGCAT 2 cut(s) 234, 276
MroI TCCGGA 1 cut(s) 984
MroXI GAANNNNTTC 1 cut(s) 965
MseI TTAA 8 cut(s) 1227, 1289, 1574, 1755, 1974, 2010, 2325, 2441
MslI CAYNNNNRTG 3 cut(s) 252, 1467, 1868
MspA1I CMGCKG 3 cut(s) 1076, 2414, 2500
MspI CCGG 4 cut(s) 855, 985, 1160, 1528
MspR9I CCNGG 7 cut(s) 567, 590, 741, 855, 973, 1161, 2171
MunI CAATTG 1 cut(s) 2618
Mva1269I GAATGC 2 cut(s) 139, 2556
MvaI CCWGG 5 cut(s) 567, 590, 741, 973, 2171
MvnI CGCG 5 cut(s) 409, 414, 658, 1523, 2500
MwoI GCNNNNNNNGC 5 cut(s) 60, 694, 1073, 2359, 2509
NciI CCSGG 2 cut(s) 855, 1161
NcoI CCATGG 1 cut(s) 1866
NdeI CATATG 1 cut(s) 2193
NdeII GATC 9 cut(s) 250, 572, 1145, 1210, 1793, 1872, 2083, 2188, 2198
NheI GCTAGC 1 cut(s) 1090
NlaIII CATG 7 cut(s) 257, 697, 926, 1466, 1870, 2303, 2602
NlaIV GGNNCC 1 cut(s) 1055
NmeAIII GCCGAG 1 cut(s) 1224
NmuCI GTSAC 4 cut(s) 316, 652, 942, 2001
NruI TCGCGA 1 cut(s) 409
NsiI ATGCAT 2 cut(s) 234, 276
NspI RCATGY 2 cut(s) 697, 2602
NspV TTCGAA 3 cut(s) 72, 153, 513
OliI CACNNNNGTG 1 cut(s) 1868
PaeR7I CTCGAG 1 cut(s) 1110
PceI AGGCCT 1 cut(s) 1540
PcsI WCGNNNNNNNCGW 1 cut(s) 655
PctI GAATGC 2 cut(s) 139, 2556
PdmI GAANNNNTTC 1 cut(s) 965
PfeI GAWTC 6 cut(s) 961, 988, 1615, 1909, 2263, 2533
Pfl23II CGTACG 1 cut(s) 399
PfoI TCCNGGA 2 cut(s) 739, 853
PkrI GCNGC 6 cut(s) 437, 768, 955, 1197, 2499, 2502
PleI GAGTC 5 cut(s) 116, 398, 1522, 1791, 2649
PpsI GAGTC 5 cut(s) 116, 398, 1522, 1791, 2649
PsiI TTATAA 1 cut(s) 1134
Psp124BI GAGCTC 1 cut(s) 2340
Psp1406I AACGTT 2 cut(s) 1324, 2322
Psp6I CCWGG 5 cut(s) 565, 588, 739, 971, 2169
PspGI CCWGG 5 cut(s) 565, 588, 739, 971, 2169
PspLI CGTACG 1 cut(s) 399
PspN4I GGNNCC 1 cut(s) 1055
PspPI GGNCC 3 cut(s) 563, 1157, 1525
PspXI VCTCGAGB 1 cut(s) 1110
PstI CTGCAG 1 cut(s) 768
PsuI RGATCY 4 cut(s) 572, 1145, 1210, 2083
PvuII CAGCTG 1 cut(s) 2414
RruI TCGCGA 1 cut(s) 409
RseI CAYNNNNRTG 3 cut(s) 252, 1467, 1868
Rsr2I CGGWCCG 1 cut(s) 1525
RsrII CGGWCCG 1 cut(s) 1525
SacI GAGCTC 1 cut(s) 2340
SacII CCGCGG 1 cut(s) 2501
SaqAI TTAA 8 cut(s) 1227, 1289, 1574, 1755, 1974, 2010, 2325, 2441
SatI GCNGC 6 cut(s) 436, 767, 954, 1196, 2498, 2501
Sau3AI GATC 9 cut(s) 250, 572, 1145, 1210, 1793, 1872, 2083, 2188, 2198
Sau96I GGNCC 3 cut(s) 563, 1157, 1525
ScaI AGTACT 1 cut(s) 1778
SchI GAGTC 5 cut(s) 117, 398, 1523, 1792, 2649
ScrFI CCNGG 7 cut(s) 567, 590, 741, 855, 973, 1161, 2171
SduI GDGCHC 3 cut(s) 853, 1166, 2340
SfaNI GCATC 5 cut(s) 219, 261, 1335, 1884, 2146
SfcI CTRYAG 2 cut(s) 764, 1436
Sfr274I CTCGAG 1 cut(s) 1110
Sfr303I CCGCGG 1 cut(s) 2501
SfuI TTCGAA 3 cut(s) 72, 153, 513
SgrBI CCGCGG 1 cut(s) 2501
SinI GGWCC 3 cut(s) 563, 1157, 1525
SlaI CTCGAG 1 cut(s) 1110
SmiMI CAYNNNNRTG 3 cut(s) 252, 1467, 1868
SmlI CTYRAG 8 cut(s) 674, 1110, 1246, 1780, 1904, 2123, 2333, 2363
SmoI CTYRAG 8 cut(s) 674, 1110, 1246, 1780, 1904, 2123, 2333, 2363
SpeI ACTAGT 1 cut(s) 875
SseBI AGGCCT 1 cut(s) 1540
SspI AATATT 2 cut(s) 1691, 1972
SstI GAGCTC 1 cut(s) 2340
StuI AGGCCT 1 cut(s) 1540
StyD4I CCNGG 7 cut(s) 565, 588, 739, 853, 971, 1159, 2169
StyI CCWWGG 3 cut(s) 1866, 2043, 2218
TaaI ACNGT 5 cut(s) 1507, 1685, 1810, 2631, 2672
TaiI ACGT 3 cut(s) 79, 1327, 2325
TaqII GACCGA 1 cut(s) 551
TatI WGTACW 6 cut(s) 478, 806, 1242, 1776, 1900, 2114
TauI GCSGC 4 cut(s) 438, 956, 2500, 2503
TfiI GAWTC 6 cut(s) 961, 988, 1615, 1909, 2263, 2533
Tru1I TTAA 8 cut(s) 1227, 1289, 1574, 1755, 1974, 2010, 2325, 2441
Tru9I TTAA 8 cut(s) 1227, 1289, 1574, 1755, 1974, 2010, 2325, 2441
TscAI CASTG 5 cut(s) 373, 940, 1417, 1575, 2010
TseFI GTSAC 4 cut(s) 316, 652, 942, 2001
TseI GCWGC 2 cut(s) 766, 1195
Tsp45I GTSAC 4 cut(s) 316, 652, 942, 2001
TspDTI ATGAA 7 cut(s) 146, 183, 311, 543, 1395, 2147, 2466
TspGWI ACGGA 3 cut(s) 4, 1457, 2502
TspRI CASTG 5 cut(s) 373, 940, 1417, 1575, 2010
VpaK11BI GGWCC 3 cut(s) 563, 1157, 1525
XbaI TCTAGA 3 cut(s) 1391, 2281, 2522
XceI RCATGY 2 cut(s) 697, 2602
XhoI CTCGAG 1 cut(s) 1110
XmiI GTMKAC 3 cut(s) 261, 1625, 2224
XmnI GAANNNNTTC 1 cut(s) 965
ZrmI AGTACT 1 cut(s) 1778
Zsp2I ATGCAT 2 cut(s) 234, 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.