RchiOBHm_Chr2g0168061

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000865
Physical Location & Seq
Forward (+)
82523244 .. 82524416
1173 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53578

Sequence Viewer

Length: 1173 bp
ATGACTCATACTCGTACCTTACTAGTTTTCCGGCAGACAGCCAATAGGTCTGAGCTTCATATGGTGCTTAAAGCCTGTAAGCTCTTACGGGTCTTCGGTTTGCAAGGTGTACCTGAATTGGTGAACTTTCCGGAATCTGTAATTAGTCTCACCTTATTAAGGTACTTGAGTGTCAGGCAAACCAACATTGAAAGTGTTCCAAGATCCATAGAGAAGCTTGGATTGTTGGAAACCTTAGAACTTAAAGACACCCTGGTGACCAATTTGCCAAAAGGGATTGGTAAACTCCACCATTTGCGCCACCTTTTGGTGTATTGCAATGCTGTCAACAATAATATGATGTTTGGAGCTGCACAAGGAGTGGAGTTTTCTACTAGCAAAATAAGTGCTTTATCTATTTCAAATTCGTTACAAAAGTTGTCTCTGATTAAGGTAAACACCAACAAAAAATTCTTGAAAGCCTTAGGAGAGTTGAAAGATCTTAGAAGGTTGGGGCTGGTAGATCTTCATAGAGAAGATGGAAGAGAATTGTGTTGCACCATTCAGAAAATGGATGATCTTTCGACATTGGATGTAAGAGCAACAAGCGAGGATGAATATCTTGACTTGGATCACATGCAATCCGGCTCCCCGCCTCTCCTTCTCCAACATCTATATTTAAAAGGGCGGCTAAAAAGGGTACCAGAGTGGATTCCCAAGCTTACCAGTCTCAAAAAGATTGGTTTAAAATGGTCAAAGATGGATGCTGACGCTGAGCCACTTACGGCCCTTCAAGATTTGCCCAACCTCATGGAGCTCGATTTGGTTGACTATTACACTGGCCAGGTGTTGGGTTTCGGAGATGAAACTTTCAAGAAACTAGTGGCATTAAGTATAGACAAATTTGATAAGCTAAATTTGATCTCAATAGGTGAGAAGGCAATGCCTATGCTCAAGAAGCTAACCATCTCGAGATGTCCGGATTTGAAGTTACTTCCATTTTCAAGTGGTGATCTTGAATTGCTGGAAGAATTGCTTCTATTTGACATGCCCAATGAATTCATTGCTGAGTTTGAAACAGACAGCGAACGACGTGAGGATTTAAAGCATGTTGGAGTCATTCATTATTCTCGGCATCCAGGAAGCTACCAATCTGATGGATTCAAAGATCTTTCTCCATCAAAATGGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

390

Amino Acids

44.3

Weight (kDa)

7.67

Isoelectric Point (pI)

40.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 3 - 317 2.7e-36 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 679
AccB1I GGYRCC 1 cut(s) 679
AccB7I CCANNNNNTGG 2 cut(s) 307, 829
AccIII TCCGGA 2 cut(s) 130, 958
AciI CCGC 2 cut(s) 632, 667
AclWI GGATC 2 cut(s) 198, 618
AcoI YGGCCR 1 cut(s) 820
AcsI RAATTY 5 cut(s) 403, 449, 881, 895, 1037
AfaI GTAC 4 cut(s) 16, 111, 164, 681
AfiI CCNNNNNNNGG 3 cut(s) 159, 307, 829
AhlI ACTAGT 2 cut(s) 22, 859
AjiI CACGTC 1 cut(s) 1073
AjnI CCWGG 3 cut(s) 252, 822, 1117
AjuI GAANNNNNNNTTGG 2 cut(s) 434, 466
AleI CACNNNNGTG 1 cut(s) 254
AluBI AGCT 9 cut(s) 55, 82, 217, 350, 700, 796, 892, 940, 1125
AluI AGCT 9 cut(s) 55, 82, 217, 350, 700, 796, 892, 940, 1125
Alw21I GWGCWC 1 cut(s) 798
Alw26I GTCTC 3 cut(s) 152, 426, 713
AlwI GGATC 2 cut(s) 198, 618
Ama87I CYCGRG 1 cut(s) 949
Aor13HI TCCGGA 2 cut(s) 130, 958
AoxI GGCC 2 cut(s) 765, 820
ApeKI GCWGC 1 cut(s) 350
ApoI RAATTY 5 cut(s) 403, 449, 881, 895, 1037
Asp700I GAANNNNTTC 2 cut(s) 195, 1014
Asp718I GGTACC 1 cut(s) 679
AspLEI GCGC 1 cut(s) 300
AspS9I GGNCC 1 cut(s) 766
AsuHPI GGTGA 5 cut(s) 133, 142, 268, 923, 1001
AvaI CYCGRG 1 cut(s) 949
AxyI CCTNAGG 1 cut(s) 463
BaeI ACNNNNGTAYC 4 cut(s) 154, 187, 671, 704
BalI TGGCCA 1 cut(s) 822
BanI GGYRCC 1 cut(s) 679
BanII GRGCYC 1 cut(s) 798
BbsI GAAGAC 1 cut(s) 85
Bbv12I GWGCWC 1 cut(s) 798
BbvI GCAGC 1 cut(s) 337
BccI CCATC 5 cut(s) 512, 733, 953, 1130, 1165
BceAI ACGGC 1 cut(s) 780
BciT130I CCWGG 3 cut(s) 254, 824, 1119
BcoDI GTCTC 3 cut(s) 152, 426, 713
BcuI ACTAGT 2 cut(s) 22, 859
BfaI CTAG 3 cut(s) 23, 375, 860
BglII AGATCT 3 cut(s) 478, 502, 1147
BisI GCNGC 2 cut(s) 351, 668
BlpI GCTNAGC 1 cut(s) 753
BlsI GCNGC 2 cut(s) 352, 669
Bme1390I CCNGG 3 cut(s) 254, 824, 1119
BmeT110I CYCGRG 1 cut(s) 949
BmgBI CACGTC 1 cut(s) 1073
BmgT120I GGNCC 1 cut(s) 766
BmiI GGNNCC 2 cut(s) 628, 681
BmrFI CCNGG 3 cut(s) 254, 824, 1119
BmsI GCATC 2 cut(s) 733, 1123
BpiI GAAGAC 1 cut(s) 85
Bpu1102I GCTNAGC 1 cut(s) 753
BpuEI CTTGAG 2 cut(s) 187, 917
BsaBI GATNNNNATC 1 cut(s) 597
BsaJI CCNNGG 1 cut(s) 252
BsaWI WCCGGW 2 cut(s) 130, 958
Bsc4I CCNNNNNNNGG 3 cut(s) 159, 307, 829
Bse1I ACTGG 2 cut(s) 705, 823
Bse21I CCTNAGG 1 cut(s) 463
Bse3DI GCAATG 3 cut(s) 325, 927, 1041
Bse8I GATNNNNATC 1 cut(s) 597
BseAI TCCGGA 2 cut(s) 130, 958
BseBI CCWGG 3 cut(s) 254, 824, 1119
BseDI CCNNGG 1 cut(s) 252
BseGI GGATG 5 cut(s) 559, 577, 598, 748, 1114
BseJI GATNNNNATC 1 cut(s) 597
BseLI CCNNNNNNNGG 3 cut(s) 159, 307, 829
BseMI GCAATG 3 cut(s) 325, 927, 1041
BseMII CTCAG 3 cut(s) 42, 744, 1038
BseNI ACTGG 2 cut(s) 705, 823
BseXI GCAGC 1 cut(s) 337
BsgI GTGCAG 1 cut(s) 336
BshFI GGCC 2 cut(s) 767, 822
BshNI GGYRCC 1 cut(s) 679
BsiHKAI GWGCWC 1 cut(s) 798
BsiHKCI CYCGRG 1 cut(s) 949
BsiSI CCGG 4 cut(s) 31, 131, 624, 959
BslI CCNNNNNNNGG 3 cut(s) 159, 307, 829
BsmAI GTCTC 3 cut(s) 152, 426, 713
BsnI GGCC 2 cut(s) 767, 822
BsoBI CYCGRG 1 cut(s) 949
Bsp1286I GDGCHC 1 cut(s) 798
Bsp13I TCCGGA 2 cut(s) 130, 958
Bsp143I GATC 8 cut(s) 203, 478, 502, 556, 610, 900, 991, 1147
Bsp1720I GCTNAGC 1 cut(s) 753
BspACI CCGC 2 cut(s) 632, 667
BspANI GGCC 2 cut(s) 767, 822
BspCNI CTCAG 3 cut(s) 43, 745, 1039
BspEI TCCGGA 2 cut(s) 130, 958
BspLI GGNNCC 2 cut(s) 628, 681
BspPI GGATC 2 cut(s) 198, 618
BspT107I GGYRCC 1 cut(s) 679
BsrDI GCAATG 3 cut(s) 325, 927, 1041
BsrI ACTGG 2 cut(s) 705, 823
BssECI CCNNGG 1 cut(s) 252
BssMI GATC 8 cut(s) 203, 478, 502, 556, 610, 900, 991, 1147
Bst2UI CCWGG 3 cut(s) 254, 824, 1119
Bst6I CTCTTC 1 cut(s) 517
BstDEI CTNAG 6 cut(s) 51, 235, 463, 482, 753, 1047
BstEII GGTNACC 1 cut(s) 256
BstENI CCTNNNNNAGG 1 cut(s) 157
BstF5I GGATG 5 cut(s) 559, 577, 598, 748, 1114
BstHHI GCGC 1 cut(s) 300
BstKTI GATC 8 cut(s) 206, 481, 505, 559, 613, 903, 994, 1150
BstMAI GTCTC 3 cut(s) 152, 426, 713
BstMBI GATC 8 cut(s) 203, 478, 502, 556, 610, 900, 991, 1147
BstMWI GCNNNNNNNGC 1 cut(s) 937
BstNI CCWGG 3 cut(s) 254, 824, 1119
BstNSI RCATGY 3 cut(s) 619, 1030, 1091
BstPI GGTNACC 1 cut(s) 256
BstSCI CCNGG 3 cut(s) 252, 822, 1117
BstV1I GCAGC 1 cut(s) 337
BstV2I GAAGAC 1 cut(s) 85
BstX2I RGATCY 4 cut(s) 203, 478, 502, 1147
BstXI CCANNNNNNTGG 3 cut(s) 790, 1136, 1164
BstYI RGATCY 4 cut(s) 203, 478, 502, 1147
Bsu36I CCTNAGG 1 cut(s) 463
BsuRI GGCC 2 cut(s) 767, 822
BtrI CACGTC 1 cut(s) 1073
BtsCI GGATG 5 cut(s) 559, 577, 598, 748, 1114
BtsIMutI CAGTG 1 cut(s) 816
CfoI GCGC 1 cut(s) 300
Cfr13I GGNCC 1 cut(s) 766
CseI GACGC 1 cut(s) 758
Csp6I GTAC 4 cut(s) 15, 110, 163, 680
CviAII CATG 4 cut(s) 616, 790, 1027, 1088
CviQI GTAC 4 cut(s) 15, 110, 163, 680
DdeI CTNAG 6 cut(s) 51, 235, 463, 482, 753, 1047
DpnI GATC 8 cut(s) 205, 480, 504, 558, 612, 902, 993, 1149
DpnII GATC 8 cut(s) 203, 478, 502, 556, 610, 900, 991, 1147
DraI TTTAAA 3 cut(s) 660, 726, 1083
EaeI YGGCCR 1 cut(s) 820
Eam1104I CTCTTC 1 cut(s) 517
EarI CTCTTC 1 cut(s) 517
Ecl136II GAGCTC 1 cut(s) 796
Eco24I GRGCYC 1 cut(s) 798
Eco53kI GAGCTC 1 cut(s) 796
Eco81I CCTNAGG 1 cut(s) 463
Eco88I CYCGRG 1 cut(s) 949
Eco91I GGTNACC 1 cut(s) 256
EcoICRI GAGCTC 1 cut(s) 796
EcoNI CCTNNNNNAGG 1 cut(s) 157
EcoO65I GGTNACC 1 cut(s) 256
EcoRI GAATTC 1 cut(s) 1037
EcoRII CCWGG 3 cut(s) 252, 822, 1117
EcoT38I GRGCYC 1 cut(s) 798
FaeI CATG 4 cut(s) 619, 793, 1030, 1091
FalI AAGNNNNNCTT 2 cut(s) 999, 1031
FatI CATG 4 cut(s) 615, 789, 1026, 1087
FauI CCCGC 1 cut(s) 639
FauNDI CATATG 1 cut(s) 60
Fnu4HI GCNGC 2 cut(s) 351, 668
FokI GGATG 5 cut(s) 566, 584, 605, 755, 1101
FriOI GRGCYC 1 cut(s) 798
Fsp4HI GCNGC 2 cut(s) 351, 668
FspBI CTAG 3 cut(s) 23, 375, 860
GlaI GCGC 1 cut(s) 299
GluI GCNGC 2 cut(s) 351, 668
HaeIII GGCC 2 cut(s) 767, 822
HapII CCGG 4 cut(s) 31, 131, 624, 959
HgaI GACGC 1 cut(s) 758
HhaI GCGC 1 cut(s) 300
Hin1II CATG 4 cut(s) 619, 793, 1030, 1091
Hin6I GCGC 1 cut(s) 298
HinP1I GCGC 1 cut(s) 298
HincII GTYRAC 2 cut(s) 328, 808
HindII GTYRAC 2 cut(s) 328, 808
HindIII AAGCTT 2 cut(s) 215, 698
HinfI GANTC 5 cut(s) 4, 134, 691, 1095, 1140
HpaII CCGG 4 cut(s) 31, 131, 624, 959
HphI GGTGA 5 cut(s) 133, 142, 268, 923, 1001
Hpy166II GTNNAC 6 cut(s) 110, 124, 284, 328, 436, 808
Hpy188I TCNGA 5 cut(s) 52, 426, 546, 839, 1135
Hpy8I GTNNAC 6 cut(s) 110, 124, 284, 328, 436, 808
Hpy99I CGWCG 1 cut(s) 1074
HpyAV CCTTC 4 cut(s) 480, 650, 779, 910
HpyCH4IV ACGT 1 cut(s) 1072
HpyCH4V TGCA 5 cut(s) 103, 318, 353, 537, 619
HpyF10VI GCNNNNNNNGC 1 cut(s) 937
HpyF3I CTNAG 6 cut(s) 51, 235, 463, 482, 753, 1047
HpySE526I ACGT 1 cut(s) 1072
Hsp92II CATG 4 cut(s) 619, 793, 1030, 1091
HspAI GCGC 1 cut(s) 298
Kpn2I TCCGGA 2 cut(s) 130, 958
KpnI GGTACC 1 cut(s) 683
Kzo9I GATC 8 cut(s) 203, 478, 502, 556, 610, 900, 991, 1147
LmnI GCTCC 3 cut(s) 347, 632, 793
Lsp1109I GCAGC 1 cut(s) 337
LweI GCATC 2 cut(s) 733, 1123
MaeI CTAG 3 cut(s) 23, 375, 860
MaeII ACGT 1 cut(s) 1072
MaeIII GTNAC 3 cut(s) 256, 408, 969
MalI GATC 8 cut(s) 205, 480, 504, 558, 612, 902, 993, 1149
MboI GATC 8 cut(s) 203, 478, 502, 556, 610, 900, 991, 1147
MboII GAAGA 5 cut(s) 85, 497, 527, 534, 1019
MflI RGATCY 4 cut(s) 203, 478, 502, 1147
MhlI GDGCHC 1 cut(s) 798
MlsI TGGCCA 1 cut(s) 822
MluNI TGGCCA 1 cut(s) 822
MlyI GAGTC 1 cut(s) 1104
MmeI TCCRAC 3 cut(s) 207, 670, 1072
MnlI CCTC 5 cut(s) 583, 645, 797, 1069, 1161
Mox20I TGGCCA 1 cut(s) 822
MroI TCCGGA 2 cut(s) 130, 958
MroXI GAANNNNTTC 2 cut(s) 195, 1014
MscI TGGCCA 1 cut(s) 822
MseI TTAA 8 cut(s) 69, 158, 243, 429, 659, 725, 869, 1082
MslI CAYNNNNRTG 2 cut(s) 254, 1162
Msp20I TGGCCA 1 cut(s) 822
MspI CCGG 4 cut(s) 31, 131, 624, 959
MspR9I CCNGG 3 cut(s) 254, 824, 1119
MvaI CCWGG 3 cut(s) 254, 824, 1119
MwoI GCNNNNNNNGC 1 cut(s) 937
NdeI CATATG 1 cut(s) 60
NdeII GATC 8 cut(s) 203, 478, 502, 556, 610, 900, 991, 1147
NlaIII CATG 4 cut(s) 619, 793, 1030, 1091
NlaIV GGNNCC 2 cut(s) 628, 681
NmeAIII GCCGAG 1 cut(s) 1090
NmuCI GTSAC 1 cut(s) 256
NspI RCATGY 3 cut(s) 619, 1030, 1091
OliI CACNNNNGTG 1 cut(s) 254
PaeR7I CTCGAG 1 cut(s) 949
PdmI GAANNNNTTC 2 cut(s) 195, 1014
PfeI GAWTC 3 cut(s) 134, 691, 1140
PflMI CCANNNNNTGG 2 cut(s) 307, 829
PfoI TCCNGGA 1 cut(s) 1117
PkrI GCNGC 2 cut(s) 352, 669
PleI GAGTC 1 cut(s) 1103
PpsI GAGTC 1 cut(s) 1103
Psp124BI GAGCTC 1 cut(s) 798
Psp6I CCWGG 3 cut(s) 252, 822, 1117
PspEI GGTNACC 1 cut(s) 256
PspGI CCWGG 3 cut(s) 252, 822, 1117
PspN4I GGNNCC 2 cut(s) 628, 681
PspPI GGNCC 1 cut(s) 766
PsuI RGATCY 4 cut(s) 203, 478, 502, 1147
RsaI GTAC 4 cut(s) 16, 111, 164, 681
RsaNI GTAC 4 cut(s) 15, 110, 163, 680
RseI CAYNNNNRTG 2 cut(s) 254, 1162
SacI GAGCTC 1 cut(s) 798
SaqAI TTAA 8 cut(s) 69, 158, 243, 429, 659, 725, 869, 1082
SatI GCNGC 2 cut(s) 351, 668
Sau3AI GATC 8 cut(s) 203, 478, 502, 556, 610, 900, 991, 1147
Sau96I GGNCC 1 cut(s) 766
SchI GAGTC 1 cut(s) 1104
ScrFI CCNGG 3 cut(s) 254, 824, 1119
SduI GDGCHC 1 cut(s) 798
SfaNI GCATC 2 cut(s) 733, 1123
Sfr274I CTCGAG 1 cut(s) 949
SlaI CTCGAG 1 cut(s) 949
SmiMI CAYNNNNRTG 2 cut(s) 254, 1162
SmlI CTYRAG 3 cut(s) 166, 932, 949
SmoI CTYRAG 3 cut(s) 166, 932, 949
SpeI ACTAGT 2 cut(s) 22, 859
SsiI CCGC 2 cut(s) 632, 667
SspMI CTAG 3 cut(s) 23, 375, 860
SstI GAGCTC 1 cut(s) 798
StyD4I CCNGG 3 cut(s) 252, 822, 1117
TaiI ACGT 1 cut(s) 1075
TaqI TCGA 3 cut(s) 563, 798, 950
TauI GCSGC 1 cut(s) 670
TfiI GAWTC 3 cut(s) 134, 691, 1140
Tru1I TTAA 8 cut(s) 69, 158, 243, 429, 659, 725, 869, 1082
Tru9I TTAA 8 cut(s) 69, 158, 243, 429, 659, 725, 869, 1082
TscAI CASTG 1 cut(s) 823
TseFI GTSAC 1 cut(s) 256
TseI GCWGC 1 cut(s) 350
Tsp45I GTSAC 1 cut(s) 256
TspDTI ATGAA 7 cut(s) 47, 497, 609, 858, 1030, 1050, 1091
TspRI CASTG 1 cut(s) 823
Van91I CCANNNNNTGG 2 cut(s) 307, 829
XagI CCTNNNNNAGG 1 cut(s) 157
XapI RAATTY 5 cut(s) 403, 449, 881, 895, 1037
XceI RCATGY 3 cut(s) 619, 1030, 1091
XcmI CCANNNNNNNNNTGG 1 cut(s) 547
XhoI CTCGAG 1 cut(s) 949
XmnI GAANNNNTTC 2 cut(s) 195, 1014
XspI CTAG 3 cut(s) 23, 375, 860
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.