Rh5BG194100

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000865
Physical Location & Seq
Forward (+)
21421010 .. 21423817
2808 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG194100.1

Sequence Viewer

Length: 2808 bp
ATGGCTGAACTTGCCATTCCTGTAGCACTGGCCGTAATTGACAAGGTGATAATCATCCTCCAGCAAAACTTTCACCGTCAAACTAAAGTCAAGGATGATGTTGAAAAAGCCCAGAAATATTTGAGGAGGATGAAAGCGTATCTAGCCGACACATCAGAAGATATCGATGAGGGTACTCTAGTGTTTCAGACTCGAGTAAGAGATATCCAAGAGTTGGCTTATGAATTTGAGGATGCTCTCGACAAGTTCATGCTTCAGGTTCCTCATCACATCCATAGCAATATATCCTCTCAGACGATACACAAAGCTGTTCATTATTTCCCAACCAGGAATGCAGGCAAGGAGTTGTCGACTGAGATTAGTGGTATTATCGAAAAAAATGTTGGTTTGATTACAGCACTAGATGCTCTTCATAACCAAGCTCCAGCACAAAGCCGCCCAAGTTCCAATTCCGGGGAAGGTTTGACCCACTACGACCATGTTCTTGAAGAAGAAGAAATAGTGGGCTTTGAGGAGCATGAAGAAAAACTCTTGGCACAGTTGATGGAAGAAAATTCAAGGCTTTTGACGATTTCGATTGTAGGCCCGGGCGGTTCGGGCAAAACCACTCTGGCAAAGAAGGTTTTCGACAACAAGCGGGTCCAAGGGTTTTTTGACTGCCAAGCTTGGGTTGATGTGCCACGCGCATTTGTACTCCATAAGGTTTTGCTAAACATTCTAAGCCAAATTGAGGCAAAAGGGCAGAAGGAACCAGTAATTCATGGAGAAAATGATCCAAAGGATAAATTGAGACTACTTCTCAAGGACAAGCGGTTTTTGGTCGTTTTGGATAATGTTTGGAACAGACCAGATTTGGAATACGTCGTAAAGGCATTACAACATGGTTCGAATGGCAGTAGAATCATCATCACTACTCCTAAAGTAATTTGTATTTCCTCTAATCTATATGTCCATGATTTGAGTCGTGGTTTACAAGGGAAAAAAGCCAAGTACCTATTCTGCAAGAAGGTTTTTCAAAGCAGTGAAGGAAGTTGTCCATTCGAGCTCGATGACTGGGCCCAACAAATTCTGAAGCGGTGTGAAGGCCTGCCCCTTGCAATTTCAGCTATTGGCACTCATCTTTCCACTAAGCCGAAAACTCCAGTTGAGTGGAAGAATTTCCATAACAGCCTTGGATCAGAAATTAGACCAGACTCAAATCTTTCGGTTATTAGCCGTATTTTACAGCCAAGTTACAAGGATCTTCCAACCCATCTGAAATCTTGTTTCCTGTACTTCAGCATATTTCCTGAAGACTACTCCATCGGGCGTCAAAGACTAATTCGTTTGTGGATAGCTGAAGGATTTGTGAAGAGCAAAAGAGGAATGACAATGGAACAGGTGGCAGAAGGTTATCTGAATGAGCTTGTTGGAAGAAATTTGGCGCATACCAGCGCCAGGGAAATCAATGGACGAGTAAGGAGTTGTCGTGTTTCGAACCTTGTTCGTGACTTCATCATTTCAAAAGCCGAGAATTTCATTAGTGTTTTGGATCAAACTGGTACTAGCGGTATTTCAGGTGAGAAAATACGCCGCCTTTCTATTCATATCAAATTGCTAGAGTCTAGTTATGACTTGAGTCATATTCGTACCCTATTACTATTCCAGCAGCAAAGTTCTCATTCTGTGCTTGAAAGGGTAGTTGAAAGATCTCGATATTTAAGGGTTTTAGATTTGCAAGGTGTGCCCTTAGAGGATTTTCCCAACAGTGTTCTTGCTCTCACTCTCTTAAAATACTTGAGTCTCAGAGAAACTAACATTAAAACAGTTCCAAAGTCCATAAAGAATCTAGCACGCTTGGAAACCTTGGATCTTAAGCACACTCAAGTCACCACTTTACCAAAAGAGATTTATGAGCTCCACAGTATGCGCCATCTTTTAGTGTATCGCTATGATGTCCAGAATTATGTGATGTTTGGAGATGCCCAAGGAGTGGAGGTTTCTCAAAGCATGCTTTCTGCAGCGAATATTGGAGCTTTATCATCTCTACAAAAGTTGTCACTAATGAAGGTGAAAAACAACAAAAAACTCATTAAAGCCTTGGGAAGGCTAACTGATCTTAGAAAGCTGGGGCTGACAGATCTAGAAAGACAAGACGGAAGGGATCTGTGTTTAGCCATTCAAAAGATGAAGCATCTTTCAACACTTGATGTAAGATCAACAAGTGTGGACGAGCCTCTTGAGTTGGATCATATGCAATCTGGATCCCCTTCACCACTCTATCTAGAGAGATTATATTTGAAAGGGAGTCTAGAAAGGTTACCACGGTGGATTCCTGAGCTTAATAGTCTAGCAAAGATTGGTTTGAAGTGGTCAAAGCTGAATGCTAAGTTTATTCACCCACTTGACGCCCTTCAAGGGTTGCCTAATCTAATGCAGCTCGAGTTGGTTGACTATTACCGAGGGGAAGTGCTAGAATTCAGTACCAATACGTTCAAGAAACTAAAGATATTACATATTGAACAATTTGAAGGGCTACATAGGATGAAAGTCCAGAGTGAAGCAATGCCTATGCTTGAGAAGCTAACCATGTGTAAATGTAGGAATTTGAAGTTTCTTCCAGAATTTGGCCCTGATGATCTGAAATCACTGGAGGAACTGCTTCTCTATGACATGCACAAGGAATTCATTGCCAAGCTTCAGAATGATTCCGAAGACCGTCTGGGGTATAAGCATGTTAGAGTCATACATTCTTTCAGTCTCGGCAGCAACCATTCTTTCACTGATTTCAAGAATCTTTCTCCATCACAGTGGCTTGTGGAACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

935

Amino Acids

106.67

Weight (kDa)

8.77

Isoelectric Point (pI)

45.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 11 - 94 3.7e-12 Rx N-terminal domain
NB-ARC PF00931 171 - 341 2.6e-30 NB-ARC domain
WHD_DRP PF23559 428 - 499 5.5e-22 Disease resistance protein Winged helix domain
LRR_14 PF23598 541 - 880 1.2e-38 Leucine-rich repeat region
LRR_8 PF13855 566 - 622 1.7e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 214, 1972, 2606
AccI GTMKAC 1 cut(s) 350
AccII CGCG 1 cut(s) 684
AciI CCGC 7 cut(s) 436, 591, 637, 811, 1075, 1548, 1573
AclWI GGATC 9 cut(s) 767, 1183, 1248, 1539, 1857, 2151, 2235, 2238, 2251
AcoI YGGCCR 1 cut(s) 30
AcuI CTGAAG 6 cut(s) 239, 1091, 1261, 1311, 1359, 2663
AcyI GRCGYC 2 cut(s) 1309, 2388
AfaI GTAC 7 cut(s) 175, 693, 992, 1274, 1543, 1630, 2464
AfiI CCNNNNNNNGG 9 cut(s) 214, 453, 667, 730, 1437, 1972, 2085, 2397, 2606
AflII CTTAAG 1 cut(s) 1853
AjnI CCWGG 2 cut(s) 326, 1436
AjuI GAANNNNNNNTTGG 6 cut(s) 366, 398, 433, 465, 980, 1012
Alw21I GWGCWC 2 cut(s) 1047, 1899
Alw26I GTCTC 3 cut(s) 784, 1787, 2744
AlwI GGATC 9 cut(s) 767, 1183, 1248, 1539, 1857, 2151, 2235, 2238, 2251
Ama87I CYCGRG 3 cut(s) 192, 586, 2420
AoxI GGCC 5 cut(s) 30, 583, 1056, 1084, 2608
ApaI GGGCCC 1 cut(s) 1060
ApeKI GCWGC 4 cut(s) 1648, 2000, 2416, 2745
Asp700I GAANNNNTTC 3 cut(s) 623, 1157, 2640
AspLEI GCGC 4 cut(s) 686, 1426, 1436, 1911
AspS9I GGNCC 5 cut(s) 584, 640, 1056, 1057, 2609
AsuC2I CCSGG 3 cut(s) 454, 587, 588
AsuHPI GGTGA 7 cut(s) 58, 65, 1571, 1861, 2062, 2244, 2369
AsuII TTCGAA 2 cut(s) 887, 1475
AvaI CYCGRG 3 cut(s) 192, 586, 2420
AvaII GGWCC 1 cut(s) 640
BaeGI GKGCMC 2 cut(s) 1060, 1728
BaeI ACNNNNGTAYC 2 cut(s) 165, 198
BamHI GGATCC 1 cut(s) 2243
BanII GRGCYC 3 cut(s) 1047, 1060, 1899
BbsI GAAGAC 2 cut(s) 1299, 2700
Bbv12I GWGCWC 2 cut(s) 1047, 1899
BbvI GCAGC 4 cut(s) 1660, 2012, 2428, 2757
BccI CCATC 5 cut(s) 538, 1260, 1310, 1920, 2791
BceAI ACGGC 2 cut(s) 17, 1200
BciT130I CCWGG 2 cut(s) 328, 1438
BcnI CCSGG 3 cut(s) 454, 587, 588
BcoDI GTCTC 3 cut(s) 784, 1787, 2744
BfmI CTRYAG 2 cut(s) 21, 1998
BfoI RGCGCY 1 cut(s) 1437
BfrI CTTAAG 1 cut(s) 1853
BglII AGATCT 2 cut(s) 1688, 2119
BisI GCNGC 6 cut(s) 436, 1573, 1649, 2001, 2417, 2746
BlsI GCNGC 6 cut(s) 437, 1574, 1650, 2002, 2418, 2747
Bme1390I CCNGG 5 cut(s) 328, 454, 587, 588, 1438
Bme18I GGWCC 1 cut(s) 640
BmeT110I CYCGRG 3 cut(s) 192, 586, 2420
BmgT120I GGNCC 5 cut(s) 584, 640, 1056, 1057, 2609
BmiI GGNNCC 5 cut(s) 261, 641, 750, 1058, 2245
BmrFI CCNGG 5 cut(s) 328, 454, 587, 588, 1438
BmrI ACTGGG 1 cut(s) 1063
BmsI GCATC 4 cut(s) 223, 394, 1951, 2182
BmuI ACTGGG 1 cut(s) 1063
BoxI GACNNNNGTC 1 cut(s) 1617
BpiI GAAGAC 2 cut(s) 1299, 2700
BpmI CTGGAG 4 cut(s) 44, 408, 1125, 2651
Bpu10I CCTNAGC 1 cut(s) 2316
Bpu14I TTCGAA 2 cut(s) 887, 1475
BpuEI CTTGAG 6 cut(s) 785, 1636, 1798, 1848, 2240, 2576
BpuMI CCSGG 3 cut(s) 454, 587, 588
Bsa29I ATCGAT 1 cut(s) 165
BsaBI GATNNNNATC 1 cut(s) 53
BsaHI GRCGYC 2 cut(s) 1309, 2388
BsaXI ACNNNNNCTCC 4 cut(s) 1453, 1483, 1962, 1992
Bsc4I CCNNNNNNNGG 9 cut(s) 214, 453, 667, 730, 1437, 1972, 2085, 2397, 2606
Bse1I ACTGG 6 cut(s) 33, 752, 1058, 1142, 1543, 2634
Bse3DI GCAATG 2 cut(s) 2550, 2667
Bse8I GATNNNNATC 1 cut(s) 53
BseBI CCWGG 2 cut(s) 328, 1438
BseCI ATCGAT 1 cut(s) 165
BseGI GGATG 6 cut(s) 54, 100, 135, 238, 270, 2529
BseJI GATNNNNATC 1 cut(s) 53
BseLI CCNNNNNNNGG 9 cut(s) 214, 453, 667, 730, 1437, 1972, 2085, 2397, 2606
BseMI GCAATG 2 cut(s) 2550, 2667
BseMII CTCAG 4 cut(s) 305, 345, 1798, 2307
BseNI ACTGG 6 cut(s) 33, 752, 1058, 1142, 1543, 2634
BseRI GAGGAG 2 cut(s) 139, 527
BseSI GKGCMC 2 cut(s) 1060, 1728
BseXI GCAGC 4 cut(s) 1660, 2012, 2428, 2757
BseYI CCCAGC 1 cut(s) 2107
Bsh1236I CGCG 1 cut(s) 684
BshFI GGCC 5 cut(s) 32, 585, 1058, 1086, 2610
BshVI ATCGAT 1 cut(s) 165
BsiHKAI GWGCWC 2 cut(s) 1047, 1899
BsiHKCI CYCGRG 3 cut(s) 192, 586, 2420
BsiSI CCGG 2 cut(s) 453, 587
BslI CCNNNNNNNGG 9 cut(s) 214, 453, 667, 730, 1437, 1972, 2085, 2397, 2606
BsmAI GTCTC 3 cut(s) 784, 1787, 2744
BsmI GAATGC 2 cut(s) 337, 2368
BsnI GGCC 5 cut(s) 32, 585, 1058, 1086, 2610
BsoBI CYCGRG 3 cut(s) 192, 586, 2420
Bsp119I TTCGAA 2 cut(s) 887, 1475
Bsp120I GGGCCC 1 cut(s) 1056
Bsp1286I GDGCHC 4 cut(s) 1047, 1060, 1728, 1899
BspACI CCGC 7 cut(s) 436, 591, 637, 811, 1075, 1548, 1573
BspANI GGCC 5 cut(s) 32, 585, 1058, 1086, 2610
BspCNI CTCAG 4 cut(s) 304, 346, 1797, 2308
BspDI ATCGAT 1 cut(s) 165
BspFNI CGCG 1 cut(s) 684
BspLI GGNNCC 5 cut(s) 261, 641, 750, 1058, 2245
BspMAI CTGCAG 1 cut(s) 2002
BspPI GGATC 9 cut(s) 767, 1183, 1248, 1539, 1857, 2151, 2235, 2238, 2251
BspQI GCTCTTC 2 cut(s) 414, 1346
BspT104I TTCGAA 2 cut(s) 887, 1475
BspTI CTTAAG 1 cut(s) 1853
BsrDI GCAATG 2 cut(s) 2550, 2667
BsrI ACTGG 6 cut(s) 33, 752, 1058, 1142, 1543, 2634
BssNI GRCGYC 2 cut(s) 1309, 2388
BssT1I CCWWGG 5 cut(s) 643, 1171, 1845, 1966, 2079
Bst2UI CCWGG 2 cut(s) 328, 1438
Bst4CI ACNGT 8 cut(s) 77, 540, 1748, 1807, 1904, 2307, 2699, 2790
Bst6I CTCTTC 2 cut(s) 414, 1346
BstACI GRCGYC 2 cut(s) 1309, 2388
BstAFI CTTAAG 1 cut(s) 1853
BstAPI GCANNNNNTGC 2 cut(s) 404, 1723
BstBI TTCGAA 2 cut(s) 887, 1475
BstC8I GCNNGC 4 cut(s) 337, 1088, 1834, 1991
BstDEI CTNAG 9 cut(s) 291, 354, 719, 1128, 1729, 1784, 2099, 2316, 2367
BstDSI CCRYGG 1 cut(s) 2303
BstEII GGTNACC 1 cut(s) 2298
BstENI CCTNNNNNAGG 1 cut(s) 2083
BstF5I GGATG 6 cut(s) 54, 100, 135, 238, 270, 2529
BstFNI CGCG 1 cut(s) 684
BstH2I RGCGCY 1 cut(s) 1437
BstHHI GCGC 4 cut(s) 686, 1426, 1436, 1911
BstMAI GTCTC 3 cut(s) 784, 1787, 2744
BstMWI GCNNNNNNNGC 7 cut(s) 11, 143, 404, 597, 1103, 1723, 2560
BstNI CCWGG 2 cut(s) 328, 1438
BstNSI RCATGY 3 cut(s) 1993, 2656, 2717
BstPAI GACNNNNGTC 1 cut(s) 1617
BstPI GGTNACC 1 cut(s) 2298
BstSCI CCNGG 5 cut(s) 326, 452, 585, 586, 1436
BstSFI CTRYAG 2 cut(s) 21, 1998
BstSLI GKGCMC 2 cut(s) 1060, 1728
BstUI CGCG 1 cut(s) 684
BstV1I GCAGC 4 cut(s) 1660, 2012, 2428, 2757
BstV2I GAAGAC 2 cut(s) 1299, 2700
BstX2I RGATCY 6 cut(s) 1240, 1688, 1849, 2119, 2143, 2243
BstXI CCANNNNNNTGG 2 cut(s) 1149, 2790
BstYI RGATCY 6 cut(s) 1240, 1688, 1849, 2119, 2143, 2243
Bsu15I ATCGAT 1 cut(s) 165
BsuRI GGCC 5 cut(s) 32, 585, 1058, 1086, 2610
BsuTUI ATCGAT 1 cut(s) 165
BtgI CCRYGG 1 cut(s) 2303
BtsCI GGATG 6 cut(s) 54, 100, 135, 238, 270, 2529
BtsI GCAGTG 1 cut(s) 1027
BtsIMutI CAGTG 6 cut(s) 26, 1027, 1753, 2627, 2760, 2795
Cac8I GCNNGC 4 cut(s) 337, 1088, 1834, 1991
CfoI GCGC 4 cut(s) 686, 1426, 1436, 1911
Cfr13I GGNCC 5 cut(s) 584, 640, 1056, 1057, 2609
Cfr9I CCCGGG 1 cut(s) 586
ClaI ATCGAT 1 cut(s) 165
CseI GACGC 2 cut(s) 1298, 2396
Csp6I GTAC 7 cut(s) 174, 692, 991, 1273, 1542, 1629, 2463
CspCI CAANNNNNGTGG 2 cut(s) 2187, 2222
CviQI GTAC 7 cut(s) 174, 692, 991, 1273, 1542, 1629, 2463
DdeI CTNAG 9 cut(s) 291, 354, 719, 1128, 1729, 1784, 2099, 2316, 2367
EaeI YGGCCR 1 cut(s) 30
Eam1104I CTCTTC 2 cut(s) 414, 1346
EarI CTCTTC 2 cut(s) 414, 1346
Ecl136II GAGCTC 2 cut(s) 1045, 1897
Eco130I CCWWGG 5 cut(s) 643, 1171, 1845, 1966, 2079
Eco147I AGGCCT 1 cut(s) 1086
Eco24I GRGCYC 3 cut(s) 1047, 1060, 1899
Eco32I GATATC 2 cut(s) 163, 205
Eco47I GGWCC 1 cut(s) 640
Eco53kI GAGCTC 2 cut(s) 1045, 1897
Eco57I CTGAAG 6 cut(s) 239, 1091, 1261, 1311, 1359, 2663
Eco88I CYCGRG 3 cut(s) 192, 586, 2420
Eco91I GGTNACC 1 cut(s) 2298
EcoICRI GAGCTC 2 cut(s) 1045, 1897
EcoNI CCTNNNNNAGG 1 cut(s) 2083
EcoO65I GGTNACC 1 cut(s) 2298
EcoRI GAATTC 2 cut(s) 2456, 2663
EcoRII CCWGG 2 cut(s) 326, 1436
EcoRV GATATC 2 cut(s) 163, 205
EcoT14I CCWWGG 5 cut(s) 643, 1171, 1845, 1966, 2079
EcoT38I GRGCYC 3 cut(s) 1047, 1060, 1899
ErhI CCWWGG 5 cut(s) 643, 1171, 1845, 1966, 2079
FauI CCCGC 1 cut(s) 630
FauNDI CATATG 1 cut(s) 2232
FblI GTMKAC 1 cut(s) 350
Fnu4HI GCNGC 6 cut(s) 436, 1573, 1649, 2001, 2417, 2746
FokI GGATG 6 cut(s) 41, 107, 142, 245, 257, 2536
FriOI GRGCYC 3 cut(s) 1047, 1060, 1899
Fsp4HI GCNGC 6 cut(s) 436, 1573, 1649, 2001, 2417, 2746
GlaI GCGC 4 cut(s) 685, 1425, 1435, 1910
GluI GCNGC 6 cut(s) 436, 1573, 1649, 2001, 2417, 2746
GsaI CCCAGC 1 cut(s) 2111
GsuI CTGGAG 4 cut(s) 44, 408, 1125, 2651
HaeII RGCGCY 1 cut(s) 1437
HaeIII GGCC 5 cut(s) 32, 585, 1058, 1086, 2610
HapII CCGG 2 cut(s) 453, 587
HgaI GACGC 2 cut(s) 1298, 2396
HhaI GCGC 4 cut(s) 686, 1426, 1436, 1911
Hin1I GRCGYC 2 cut(s) 1309, 2388
Hin6I GCGC 4 cut(s) 684, 1424, 1434, 1909
HinP1I GCGC 4 cut(s) 684, 1424, 1434, 1909
HincII GTYRAC 2 cut(s) 351, 2431
HindII GTYRAC 2 cut(s) 351, 2431
HindIII AAGCTT 2 cut(s) 663, 2675
HpaII CCGG 2 cut(s) 453, 587
HphI GGTGA 7 cut(s) 58, 65, 1571, 1861, 2062, 2244, 2369
Hpy166II GTNNAC 4 cut(s) 351, 971, 2209, 2431
Hpy8I GTNNAC 4 cut(s) 351, 971, 2209, 2431
Hpy99I CGWCG 1 cut(s) 866
HpyCH4III ACNGT 8 cut(s) 77, 540, 1748, 1807, 1904, 2307, 2699, 2790
HpyCH4IV ACGT 2 cut(s) 861, 2471
HpyCH4V TGCA 8 cut(s) 335, 1002, 1097, 1717, 2000, 2236, 2416, 2656
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 143, 404, 597, 1103, 1723, 2560
HpyF3I CTNAG 9 cut(s) 291, 354, 719, 1128, 1729, 1784, 2099, 2316, 2367
HpySE526I ACGT 2 cut(s) 861, 2471
Hsp92I GRCGYC 2 cut(s) 1309, 2388
HspAI GCGC 4 cut(s) 684, 1424, 1434, 1909
LguI GCTCTTC 2 cut(s) 414, 1346
LmnI GCTCC 4 cut(s) 427, 514, 1902, 2012
Lsp1109I GCAGC 4 cut(s) 1660, 2012, 2428, 2757
LweI GCATC 4 cut(s) 223, 394, 1951, 2182
MaeII ACGT 2 cut(s) 861, 2471
MaeIII GTNAC 5 cut(s) 1232, 1487, 1867, 2037, 2298
MflI RGATCY 6 cut(s) 1240, 1688, 1849, 2119, 2143, 2243
MhlI GDGCHC 4 cut(s) 1047, 1060, 1728, 1899
MlyI GAGTC 8 cut(s) 184, 970, 1187, 1610, 1627, 1789, 2296, 2730
MmeI TCCRAC 3 cut(s) 1271, 1390, 2205
MroXI GAANNNNTTC 3 cut(s) 623, 1157, 2640
MseI TTAA 6 cut(s) 1700, 1769, 1800, 1854, 2073, 2322
MslI CAYNNNNRTG 1 cut(s) 2788
MspCI CTTAAG 1 cut(s) 1853
MspI CCGG 2 cut(s) 453, 587
MspR9I CCNGG 5 cut(s) 328, 454, 587, 588, 1438
Mva1269I GAATGC 2 cut(s) 337, 2368
MvaI CCWGG 2 cut(s) 328, 1438
MvnI CGCG 1 cut(s) 684
MwoI GCNNNNNNNGC 7 cut(s) 11, 143, 404, 597, 1103, 1723, 2560
NciI CCSGG 3 cut(s) 454, 587, 588
NdeI CATATG 1 cut(s) 2232
NlaIV GGNNCC 5 cut(s) 261, 641, 750, 1058, 2245
NmeAIII GCCGAG 2 cut(s) 1534, 2721
NmuCI GTSAC 3 cut(s) 1487, 1867, 2037
NspI RCATGY 3 cut(s) 1993, 2656, 2717
NspV TTCGAA 2 cut(s) 887, 1475
PaeI GCATGC 1 cut(s) 1993
PaeR7I CTCGAG 2 cut(s) 192, 2420
PceI AGGCCT 1 cut(s) 1086
PciSI GCTCTTC 2 cut(s) 414, 1346
PctI GAATGC 2 cut(s) 337, 2368
PdmI GAANNNNTTC 3 cut(s) 623, 1157, 2640
PfeI GAWTC 5 cut(s) 900, 1825, 2311, 2687, 2773
PflMI CCANNNNNTGG 3 cut(s) 214, 1972, 2606
PkrI GCNGC 6 cut(s) 437, 1574, 1650, 2002, 2418, 2747
PleI GAGTC 8 cut(s) 184, 969, 1187, 1609, 1626, 1788, 2295, 2729
PpsI GAGTC 8 cut(s) 184, 969, 1187, 1609, 1626, 1788, 2295, 2729
PshAI GACNNNNGTC 1 cut(s) 1617
Psp124BI GAGCTC 2 cut(s) 1047, 1899
Psp6I CCWGG 2 cut(s) 326, 1436
PspEI GGTNACC 1 cut(s) 2298
PspFI CCCAGC 1 cut(s) 2107
PspGI CCWGG 2 cut(s) 326, 1436
PspN4I GGNNCC 5 cut(s) 261, 641, 750, 1058, 2245
PspOMI GGGCCC 1 cut(s) 1056
PspPI GGNCC 5 cut(s) 584, 640, 1056, 1057, 2609
PspXI VCTCGAGB 2 cut(s) 192, 2420
PstI CTGCAG 1 cut(s) 2002
PsuI RGATCY 6 cut(s) 1240, 1688, 1849, 2119, 2143, 2243
RsaI GTAC 7 cut(s) 175, 693, 992, 1274, 1543, 1630, 2464
RsaNI GTAC 7 cut(s) 174, 692, 991, 1273, 1542, 1629, 2463
RseI CAYNNNNRTG 1 cut(s) 2788
SacI GAGCTC 2 cut(s) 1047, 1899
SalI GTCGAC 1 cut(s) 349
SapI GCTCTTC 2 cut(s) 414, 1346
SaqAI TTAA 6 cut(s) 1700, 1769, 1800, 1854, 2073, 2322
SatI GCNGC 6 cut(s) 436, 1573, 1649, 2001, 2417, 2746
Sau96I GGNCC 5 cut(s) 584, 640, 1056, 1057, 2609
SchI GAGTC 8 cut(s) 184, 970, 1187, 1610, 1627, 1789, 2296, 2730
ScrFI CCNGG 5 cut(s) 328, 454, 587, 588, 1438
SduI GDGCHC 4 cut(s) 1047, 1060, 1728, 1899
SfaNI GCATC 4 cut(s) 223, 394, 1951, 2182
SfcI CTRYAG 2 cut(s) 21, 1998
Sfr274I CTCGAG 2 cut(s) 192, 2420
SfuI TTCGAA 2 cut(s) 887, 1475
SinI GGWCC 1 cut(s) 640
SlaI CTCGAG 2 cut(s) 192, 2420
SmaI CCCGGG 1 cut(s) 588
SmiMI CAYNNNNRTG 1 cut(s) 2788
SmlI CTYRAG 9 cut(s) 192, 800, 1615, 1777, 1853, 1863, 2219, 2420, 2555
SmoI CTYRAG 9 cut(s) 192, 800, 1615, 1777, 1853, 1863, 2219, 2420, 2555
SphI GCATGC 1 cut(s) 1993
SrfI GCCCGGGC 1 cut(s) 588
SseBI AGGCCT 1 cut(s) 1086
SsiI CCGC 7 cut(s) 436, 591, 637, 811, 1075, 1548, 1573
SspI AATATT 2 cut(s) 119, 2008
SstI GAGCTC 2 cut(s) 1047, 1899
StuI AGGCCT 1 cut(s) 1086
StyD4I CCNGG 5 cut(s) 326, 452, 585, 586, 1436
StyI CCWWGG 5 cut(s) 643, 1171, 1845, 1966, 2079
TaaI ACNGT 8 cut(s) 77, 540, 1748, 1807, 1904, 2307, 2699, 2790
TaiI ACGT 2 cut(s) 864, 2474
TatI WGTACW 2 cut(s) 691, 1272
TauI GCSGC 2 cut(s) 438, 1575
TfiI GAWTC 5 cut(s) 900, 1825, 2311, 2687, 2773
Tru1I TTAA 6 cut(s) 1700, 1769, 1800, 1854, 2073, 2322
Tru9I TTAA 6 cut(s) 1700, 1769, 1800, 1854, 2073, 2322
TscAI CASTG 6 cut(s) 33, 1027, 1753, 2634, 2767, 2795
TseFI GTSAC 3 cut(s) 1487, 1867, 2037
TseI GCWGC 4 cut(s) 1648, 2000, 2416, 2745
Tsp45I GTSAC 3 cut(s) 1487, 1867, 2037
TspGWI ACGGA 1 cut(s) 2151
TspMI CCCGGG 1 cut(s) 586
TspRI CASTG 6 cut(s) 33, 1027, 1753, 2634, 2767, 2795
Van91I CCANNNNNTGG 3 cut(s) 214, 1972, 2606
Vha464I CTTAAG 1 cut(s) 1853
VpaK11BI GGWCC 1 cut(s) 640
XagI CCTNNNNNAGG 1 cut(s) 2083
XbaI TCTAGA 3 cut(s) 2122, 2263, 2290
XceI RCATGY 3 cut(s) 1993, 2656, 2717
XcmI CCANNNNNNNNNTGG 1 cut(s) 1169
XhoI CTCGAG 2 cut(s) 192, 2420
XmaI CCCGGG 1 cut(s) 586
XmiI GTMKAC 1 cut(s) 350
XmnI GAANNNNTTC 3 cut(s) 623, 1157, 2640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.