RchiOBHm_Chr2g0127051

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000865
Physical Location & Seq
Reverse (-)
41772289 .. 41773629
1341 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49895

Sequence Viewer

Length: 1341 bp
ATGACTGAAGTTGCTGTTCCAGCGTCGTTGGCGTCGGTGGAGAAGATGAAACTCTTCCTCTACCAACTATCTGACTGTCCCAGTAAAGTCCATGAGGAGGTAAAGAGTGCGAAGAGGTGTTTGGAGATACTGCAAGCGTATCTAGCCGACTCAAAAGATAATAATGTAGAAGACAGCCAGATGCTCAAAGATTGTTCGGATATAGCTCATGATATTGAGGACGCTCTTGATGAGTTCAATTCTGAAGTTGCACCACTGTTCCATAGCAACAAATTATCTCACAAGATTCAGCACGTGTCTCATATCCTCCAACTACGCAAAGCAAGCACTAAGTTGTCCTCGAAAATTAACGAACAGATTAAATTGGTCAAAAGATTACATTTTAAAGACTCCAGCTTCGAGGGCGACCTTGACCCAAAAGCAAGTATCTGGAAAGGCAGAACAGAATTGGATTTGACTCCAAAGATTCAAGAAGATGAAATGGTGGGCTATGAAGAATCTAAAGCAAGACTCCACAAACAGTTGATGGAAGAAGATTCAAGACTTCTGACGATTTCAATTGTAGGTCCTGGAGGCTCCGGCAAAACCACTCTCGTGAAGAATCTTTACAACATGGTCCAAGGGGTTTTTGATTCCCGTTATTGGATCGATGTGTCACGTCCCCTAAAAATGGAAGATCTCTTGCTTGCCATGTTAAACAATTTTGAGGCCACAACGCTAGATCTTCATCAAGAACAAGATCCAAGATCGAAATTGAGTCTACTGCTGCAGCACAAGAGGTTTCTGCTCGTCTTGGACAATGTTTGGAGTAAACAAGATTGGGAATTCTTTGTATGTTCTTTACCAAAAGGTTTGTGTGGGAGTAAGATAATCATTATTACTCGTAACTCTGACGTGGCTTCGTTTCCTGTAAAGTCCTCGGAGTATATCCATGATTTGAGTACTGGGTTGTCATTCAAAGAAGCCCATGACCTATTCTGTAAGAAGGCTTTTCAAGATGGGATTTGTCCCCCCGAGCTCGAGGAGTGCAGTAGAAAAATCCTGAACAAGTGTGAAGGCTCACCCTTTGCAATTTCAACTGTCGGTACTGTGCTAGCAACAAAGCCACAAACGAAAAATGAGTGGGAGGAGTTACATGACACCCTCACATCAAACTTTACAGCTGACCCAAAGCTTTGGTCCTTAGCCGTATTTTTCAGCCTTGTTACAGAGATCTTCAAAGCCATCTCAAAAGTTGCTTCTTGTACCTCGGCATATTTCCTGAAGACTACTACATCCGGCGAGGGAGGCTTTTTCGTTTGTGGGTTGCTGAAGGATTTGTGGAGCCGACAGCAGGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

446

Amino Acids

50.37

Weight (kDa)

5.86

Isoelectric Point (pI)

45.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 28 - 86 1e-07 Rx N-terminal domain
NB-ARC PF00931 165 - 333 2.5e-34 NB-ARC domain
AAA_22 PF13401 185 - 268 5.7e-06 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 760
AclWI GGATC 2 cut(s) 653, 734
AcsI RAATTY 1 cut(s) 824
AcuI CTGAAG 4 cut(s) 27, 264, 1283, 1331
AcvI CACGTG 1 cut(s) 295
AcyI GRCGYC 1 cut(s) 32
AfaI GTAC 3 cut(s) 943, 1087, 1246
AfiI CCNNNNNNNGG 4 cut(s) 97, 642, 670, 1333
AflIII ACRYGT 1 cut(s) 294
AgsI TTSAA 8 cut(s) 238, 470, 540, 558, 958, 995, 1077, 1219
AjiI CACGTC 2 cut(s) 659, 895
AjnI CCWGG 1 cut(s) 568
AjuI GAANNNNNNNTTGG 2 cut(s) 104, 136
AleI CACNNNNGTG 1 cut(s) 593
AluBI AGCT 5 cut(s) 206, 396, 1018, 1163, 1174
AluI AGCT 5 cut(s) 206, 396, 1018, 1163, 1174
Alw21I GWGCWC 1 cut(s) 1020
Alw26I GTCTC 1 cut(s) 303
AlwI GGATC 2 cut(s) 653, 734
Ama87I CYCGRG 2 cut(s) 1013, 1019
AoxI GGCC 1 cut(s) 708
ApeKI GCWGC 2 cut(s) 766, 769
ApoI RAATTY 1 cut(s) 824
ArsI GACNNNNNNTTYG 4 cut(s) 380, 412, 1163, 1195
Asp700I GAANNNNTTC 1 cut(s) 53
AspS9I GGNCC 3 cut(s) 566, 616, 1179
AsuHPI GGTGA 1 cut(s) 1053
AsuNHI GCTAGC 1 cut(s) 1093
AvaI CYCGRG 2 cut(s) 1013, 1019
AvaII GGWCC 3 cut(s) 566, 616, 1179
BanII GRGCYC 1 cut(s) 1020
BarI GAAGNNNNNNTAC 2 cut(s) 590, 622
BauI CACGAG 1 cut(s) 593
BbrPI CACGTG 1 cut(s) 295
BbsI GAAGAC 2 cut(s) 177, 1271
Bbv12I GWGCWC 1 cut(s) 1020
BbvI GCAGC 2 cut(s) 753, 781
BccI CCATC 3 cut(s) 520, 992, 1232
BceAI ACGGC 1 cut(s) 1172
BciT130I CCWGG 1 cut(s) 570
BcoDI GTCTC 1 cut(s) 303
BfaI CTAG 3 cut(s) 143, 719, 1094
BfmI CTRYAG 1 cut(s) 767
BglII AGATCT 3 cut(s) 676, 721, 1212
BisI GCNGC 2 cut(s) 767, 770
BlsI GCNGC 2 cut(s) 768, 771
BmcAI AGTACT 1 cut(s) 943
Bme1390I CCNGG 1 cut(s) 570
Bme18I GGWCC 3 cut(s) 566, 616, 1179
BmeT110I CYCGRG 2 cut(s) 1013, 1019
BmgBI CACGTC 2 cut(s) 659, 895
BmgT120I GGNCC 3 cut(s) 566, 616, 1179
BmiI GGNNCC 2 cut(s) 577, 1325
BmrFI CCNGG 1 cut(s) 570
BmrI ACTGGG 2 cut(s) 75, 954
BmsI GCATC 1 cut(s) 171
BmtI GCTAGC 1 cut(s) 1097
BmuI ACTGGG 2 cut(s) 75, 954
BpiI GAAGAC 2 cut(s) 177, 1271
BpmI CTGGAG 2 cut(s) 376, 591
Bpu10I CCTNAGC 1 cut(s) 1183
Bsa29I ATCGAT 1 cut(s) 648
BsaAI YACGTR 1 cut(s) 295
BsaBI GATNNNNATC 1 cut(s) 726
BsaHI GRCGYC 1 cut(s) 32
BsaJI CCNNGG 3 cut(s) 619, 918, 1248
Bsc4I CCNNNNNNNGG 4 cut(s) 97, 642, 670, 1333
Bse1I ACTGG 2 cut(s) 81, 949
Bse8I GATNNNNATC 1 cut(s) 726
BseBI CCWGG 1 cut(s) 570
BseCI ATCGAT 1 cut(s) 648
BseDI CCNNGG 3 cut(s) 619, 918, 1248
BseGI GGATG 1 cut(s) 1274
BseJI GATNNNNATC 1 cut(s) 726
BseLI CCNNNNNNNGG 4 cut(s) 97, 642, 670, 1333
BseNI ACTGG 2 cut(s) 81, 949
BseRI GAGGAG 3 cut(s) 110, 1037, 1142
BseXI GCAGC 2 cut(s) 753, 781
BsgI GTGCAG 1 cut(s) 1048
BshFI GGCC 1 cut(s) 710
BshVI ATCGAT 1 cut(s) 648
BsiHKAI GWGCWC 1 cut(s) 1020
BsiHKCI CYCGRG 2 cut(s) 1013, 1019
BsiSI CCGG 2 cut(s) 579, 1278
BslFI GGGAC 3 cut(s) 63, 645, 993
BslI CCNNNNNNNGG 4 cut(s) 97, 642, 670, 1333
BsmAI GTCTC 1 cut(s) 303
BsmFI GGGAC 3 cut(s) 63, 645, 993
BsnI GGCC 1 cut(s) 710
BsoBI CYCGRG 2 cut(s) 1013, 1019
Bsp1286I GDGCHC 1 cut(s) 1020
Bsp143I GATC 6 cut(s) 645, 676, 721, 739, 746, 1212
BspANI GGCC 1 cut(s) 710
BspDI ATCGAT 1 cut(s) 648
BspHI TCATGA 1 cut(s) 208
BspLI GGNNCC 2 cut(s) 577, 1325
BspMAI CTGCAG 1 cut(s) 771
BspOI GCTAGC 1 cut(s) 1097
BspPI GGATC 2 cut(s) 653, 734
BsrI ACTGG 2 cut(s) 81, 949
BssECI CCNNGG 3 cut(s) 619, 918, 1248
BssMI GATC 6 cut(s) 645, 676, 721, 739, 746, 1212
BssNI GRCGYC 1 cut(s) 32
BssSI CACGAG 1 cut(s) 593
BssT1I CCWWGG 1 cut(s) 619
Bst2BI CACGAG 1 cut(s) 593
Bst2UI CCWGG 1 cut(s) 570
Bst4CI ACNGT 5 cut(s) 77, 258, 522, 1081, 1090
Bst6I CTCTTC 2 cut(s) 59, 107
BstACI GRCGYC 1 cut(s) 32
BstBAI YACGTR 1 cut(s) 295
BstC8I GCNNGC 4 cut(s) 135, 325, 687, 1095
BstDEI CTNAG 2 cut(s) 330, 1183
BstF5I GGATG 1 cut(s) 1274
BstKTI GATC 6 cut(s) 648, 679, 724, 742, 749, 1215
BstMAI GTCTC 1 cut(s) 303
BstMBI GATC 6 cut(s) 645, 676, 721, 739, 746, 1212
BstMWI GCNNNNNNNGC 6 cut(s) 20, 29, 143, 324, 402, 1287
BstNI CCWGG 1 cut(s) 570
BstSCI CCNGG 1 cut(s) 568
BstSFI CTRYAG 1 cut(s) 767
BstV1I GCAGC 2 cut(s) 753, 781
BstV2I GAAGAC 2 cut(s) 177, 1271
BstX2I RGATCY 4 cut(s) 676, 721, 739, 1212
BstXI CCANNNNNNTGG 1 cut(s) 1176
BstYI RGATCY 4 cut(s) 676, 721, 739, 1212
Bsu15I ATCGAT 1 cut(s) 648
BsuRI GGCC 1 cut(s) 710
BsuTUI ATCGAT 1 cut(s) 648
BtrI CACGTC 2 cut(s) 659, 895
BtsCI GGATG 1 cut(s) 1274
BtsIMutI CAGTG 1 cut(s) 254
Cac8I GCNNGC 4 cut(s) 135, 325, 687, 1095
CciI TCATGA 1 cut(s) 208
Cfr13I GGNCC 3 cut(s) 566, 616, 1179
ClaI ATCGAT 1 cut(s) 648
CseI GACGC 3 cut(s) 12, 21, 230
Csp6I GTAC 3 cut(s) 942, 1086, 1245
CviAII CATG 7 cut(s) 92, 209, 613, 691, 932, 968, 1136
CviQI GTAC 3 cut(s) 942, 1086, 1245
DdeI CTNAG 2 cut(s) 330, 1183
DpnI GATC 6 cut(s) 647, 678, 723, 741, 748, 1214
DpnII GATC 6 cut(s) 645, 676, 721, 739, 746, 1212
DraI TTTAAA 1 cut(s) 385
Eam1104I CTCTTC 2 cut(s) 59, 107
EarI CTCTTC 2 cut(s) 59, 107
Ecl136II GAGCTC 1 cut(s) 1018
Eco130I CCWWGG 1 cut(s) 619
Eco24I GRGCYC 1 cut(s) 1020
Eco47I GGWCC 3 cut(s) 566, 616, 1179
Eco53kI GAGCTC 1 cut(s) 1018
Eco57I CTGAAG 4 cut(s) 27, 264, 1283, 1331
Eco72I CACGTG 1 cut(s) 295
Eco88I CYCGRG 2 cut(s) 1013, 1019
EcoICRI GAGCTC 1 cut(s) 1018
EcoO109I RGGNCCY 1 cut(s) 566
EcoRI GAATTC 1 cut(s) 824
EcoRII CCWGG 1 cut(s) 568
EcoT14I CCWWGG 1 cut(s) 619
EcoT38I GRGCYC 1 cut(s) 1020
ErhI CCWWGG 1 cut(s) 619
FaeI CATG 7 cut(s) 95, 212, 616, 694, 935, 971, 1139
FaqI GGGAC 3 cut(s) 63, 645, 993
FatI CATG 7 cut(s) 91, 208, 612, 690, 931, 967, 1135
FblI GTMKAC 1 cut(s) 760
Fnu4HI GCNGC 2 cut(s) 767, 770
FokI GGATG 1 cut(s) 1261
FriOI GRGCYC 1 cut(s) 1020
Fsp4HI GCNGC 2 cut(s) 767, 770
FspBI CTAG 3 cut(s) 143, 719, 1094
GluI GCNGC 2 cut(s) 767, 770
GsuI CTGGAG 2 cut(s) 376, 591
HaeIII GGCC 1 cut(s) 710
HapII CCGG 2 cut(s) 579, 1278
HgaI GACGC 3 cut(s) 12, 21, 230
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 7 cut(s) 95, 212, 616, 694, 935, 971, 1139
HindIII AAGCTT 1 cut(s) 1172
HpaII CCGG 2 cut(s) 579, 1278
HphI GGTGA 1 cut(s) 1053
Hpy166II GTNNAC 2 cut(s) 761, 812
Hpy188I TCNGA 6 cut(s) 73, 199, 244, 549, 892, 922
Hpy8I GTNNAC 2 cut(s) 761, 812
Hpy99I CGWCG 2 cut(s) 28, 37
HpyAV CCTTC 3 cut(s) 979, 1049, 1306
HpyCH4III ACNGT 5 cut(s) 77, 258, 522, 1081, 1090
HpyCH4IV ACGT 3 cut(s) 294, 658, 894
HpyCH4V TGCA 5 cut(s) 133, 251, 769, 1029, 1070
HpyF10VI GCNNNNNNNGC 6 cut(s) 20, 29, 143, 324, 402, 1287
HpyF3I CTNAG 2 cut(s) 330, 1183
HpySE526I ACGT 3 cut(s) 294, 658, 894
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 7 cut(s) 95, 212, 616, 694, 935, 971, 1139
Kzo9I GATC 6 cut(s) 645, 676, 721, 739, 746, 1212
LmnI GCTCC 2 cut(s) 581, 1323
Lsp1109I GCAGC 2 cut(s) 753, 781
LweI GCATC 1 cut(s) 171
MaeI CTAG 3 cut(s) 143, 719, 1094
MaeII ACGT 3 cut(s) 294, 658, 894
MaeIII GTNAC 4 cut(s) 654, 884, 1131, 1204
MalI GATC 6 cut(s) 647, 678, 723, 741, 748, 1214
MboI GATC 6 cut(s) 645, 676, 721, 739, 746, 1212
MfeI CAATTG 1 cut(s) 558
MflI RGATCY 4 cut(s) 676, 721, 739, 1212
MhlI GDGCHC 1 cut(s) 1020
MlyI GAGTC 5 cut(s) 143, 383, 451, 504, 766
MmeI TCCRAC 1 cut(s) 334
MroXI GAANNNNTTC 1 cut(s) 53
MseI TTAA 4 cut(s) 348, 360, 384, 695
MslI CAYNNNNRTG 1 cut(s) 593
MspA1I CMGCKG 1 cut(s) 1163
MspI CCGG 2 cut(s) 579, 1278
MspR9I CCNGG 1 cut(s) 570
MunI CAATTG 1 cut(s) 558
MvaI CCWGG 1 cut(s) 570
MwoI GCNNNNNNNGC 6 cut(s) 20, 29, 143, 324, 402, 1287
NdeII GATC 6 cut(s) 645, 676, 721, 739, 746, 1212
NheI GCTAGC 1 cut(s) 1093
NlaIII CATG 7 cut(s) 95, 212, 616, 694, 935, 971, 1139
NlaIV GGNNCC 2 cut(s) 577, 1325
NmeAIII GCCGAG 1 cut(s) 1229
NmuCI GTSAC 1 cut(s) 654
OliI CACNNNNGTG 1 cut(s) 593
PaeR7I CTCGAG 1 cut(s) 1019
PagI TCATGA 1 cut(s) 208
PdmI GAANNNNTTC 1 cut(s) 53
PfeI GAWTC 6 cut(s) 286, 466, 497, 536, 601, 632
PfoI TCCNGGA 1 cut(s) 568
PkrI GCNGC 2 cut(s) 768, 771
PleI GAGTC 5 cut(s) 143, 383, 451, 504, 765
PmaCI CACGTG 1 cut(s) 295
PmlI CACGTG 1 cut(s) 295
PpsI GAGTC 5 cut(s) 143, 383, 451, 504, 765
Ppu21I YACGTR 1 cut(s) 295
PpuMI RGGWCCY 1 cut(s) 566
Psp124BI GAGCTC 1 cut(s) 1020
Psp5II RGGWCCY 1 cut(s) 566
Psp6I CCWGG 1 cut(s) 568
PspCI CACGTG 1 cut(s) 295
PspGI CCWGG 1 cut(s) 568
PspN4I GGNNCC 2 cut(s) 577, 1325
PspPI GGNCC 3 cut(s) 566, 616, 1179
PspPPI RGGWCCY 1 cut(s) 566
PspXI VCTCGAGB 1 cut(s) 1019
PstI CTGCAG 1 cut(s) 771
PsuI RGATCY 4 cut(s) 676, 721, 739, 1212
PvuII CAGCTG 1 cut(s) 1163
RsaI GTAC 3 cut(s) 943, 1087, 1246
RsaNI GTAC 3 cut(s) 942, 1086, 1245
RseI CAYNNNNRTG 1 cut(s) 593
SacI GAGCTC 1 cut(s) 1020
SaqAI TTAA 4 cut(s) 348, 360, 384, 695
SatI GCNGC 2 cut(s) 767, 770
Sau3AI GATC 6 cut(s) 645, 676, 721, 739, 746, 1212
Sau96I GGNCC 3 cut(s) 566, 616, 1179
ScaI AGTACT 1 cut(s) 943
SchI GAGTC 5 cut(s) 143, 383, 451, 504, 766
ScrFI CCNGG 1 cut(s) 570
SduI GDGCHC 1 cut(s) 1020
SfaNI GCATC 1 cut(s) 171
SfcI CTRYAG 1 cut(s) 767
Sfr274I CTCGAG 1 cut(s) 1019
SinI GGWCC 3 cut(s) 566, 616, 1179
SlaI CTCGAG 1 cut(s) 1019
SmiMI CAYNNNNRTG 1 cut(s) 593
SmlI CTYRAG 1 cut(s) 1019
SmoI CTYRAG 1 cut(s) 1019
SspMI CTAG 3 cut(s) 143, 719, 1094
SstI GAGCTC 1 cut(s) 1020
StyD4I CCNGG 1 cut(s) 568
StyI CCWWGG 1 cut(s) 619
TaaI ACNGT 5 cut(s) 77, 258, 522, 1081, 1090
TaiI ACGT 3 cut(s) 297, 661, 897
TaqI TCGA 5 cut(s) 341, 399, 648, 749, 1020
TatI WGTACW 1 cut(s) 941
TfiI GAWTC 6 cut(s) 286, 466, 497, 536, 601, 632
Tru1I TTAA 4 cut(s) 348, 360, 384, 695
Tru9I TTAA 4 cut(s) 348, 360, 384, 695
TscAI CASTG 1 cut(s) 261
TseFI GTSAC 1 cut(s) 654
TseI GCWGC 2 cut(s) 766, 769
Tsp45I GTSAC 1 cut(s) 654
TspDTI ATGAA 4 cut(s) 62, 492, 507, 716
TspRI CASTG 1 cut(s) 261
VpaK11BI GGWCC 3 cut(s) 566, 616, 1179
XapI RAATTY 1 cut(s) 824
XhoI CTCGAG 1 cut(s) 1019
XmiI GTMKAC 1 cut(s) 760
XmnI GAANNNNTTC 1 cut(s) 53
XspI CTAG 3 cut(s) 143, 719, 1094
ZrmI AGTACT 1 cut(s) 943
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.