Rmu_sc0003033.1_g000048

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003033.1
OG0000865
Physical Location & Seq
Reverse (-)
194580 .. 198778
4199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003033.1_g000048.1.cds

Sequence Viewer

Length: 3105 bp
atgcttgctagcatgacggggatgatggatggacagtggaatggtcagcacttgcttggagcggcgggcacgccaacggtgataaggagaggtggtgatgctcgccgaaaactggagctgagattagggaggaggaaaagaaggccgaagagcagagaggagggagaggaagaagagagcccatcttcctatagttttccgaatcatccgtcaagtctttccattaccctctccttgtccaaaatcttcatccaatccgtttctgtagcaatattattagttgagaaggtgataagctctttggaccaaaagatcactagtaatgtgcatggtgatgttgaaaaggcctggaaatgtttgaggaggatgcaagcctatctaagggactcggaagagatttacgtaggtagtcaaatgtttcaaaatcgtgtcaaagaggtccaaagtttagcctatgaaattgaggatgctattgataagtttatggttcagattccacatcacttccacagcaatatgttctctgaggcgattcacgacattgcctactttttcatagaaagaagagcaataagtgagttctcctcaaaaattaaagatattgaaacgagagttggtagcttttcagccttggattcactacttgttgagccaacaagttctagcacaagaacagaagaaaacctgacccaccaagttctcgaggaagatgaggtagtgggctttgaagagcgtaaaggaacactcctcaaacagttgacagaagcaaattcaaagctcttcacgattttgattgtaggtccaggcggctccggcaaaaccacaatcgtgaagaatgtttatgacagtaaactggtacaaggattttttgattgccattcttggattgatgtgtcacgtcctcctaaacttggaaagctcttgcgtaatatgttaatcgatttcagactgaaaaagaataacagcgcttggagtaaatatgaagctgttagacagattgttccaagatttccgcagcaaaaactgaaaagacaaaaaccagttcatgatgaagaagaagaagaagaagaagaagacgaagaagaagaaaaaaaggaagaagaagaagcagctgaaaatccaaaagcagaattgaataattttctgaaagctaagaggtttctgcttgtgttagataatgtttggagagtacaagatctggaatccattgtaaatgctctaccaaaaggattgcctgggagcaaaataatcataactacacggaaatctgatgtagctccggttgccacatatatccatgatttgagttgtggtttgtcctggaaatatgcctggtatctgttctgcaagaaggcttttcaaagtagtgaagaaaattgtccacgcgagctcgagcactgggctaaaaaaatcctgaagaagtgtgaaggcttgccccttgctatttcagctgttggtactttgctttggaagaagccacaaactccagttgagtggaagaagttacatgaaagccttggatctgaaattagacccgattctagtctttctgttattagccgcattttgcagcccagttatatggatcttccaagccatctgaaaacttgtttcttatactttggcatgtttccagaagactatttaatcagtcgcgagaagctgactcggttgtggttagccgaagggtttgtgatgccaggaagaggaaaaacaatggaggaggttgcagagagttatctcaatgaactcattgcaagaaacttggttaatgccaccaggagagagatagatggtcgagtgaggagttgtcgtgttttaagccttgtcagagagttcatcattaccaaggcagaaaactttatcactgttttggagcccaactgcagtactgcaacttcaggtgagaaaattcgtcgcctttctgttcaaaatgtcgaaatcaatttgctattaagagatagggacttgagtcacattcgtaccttgctagtgtttgggcaggcatgttccccttctgatcttgaaatggtacttaaaacctctaaatttttgagggtgtttgatcttcaaggagtacctttgaagatttttcccaaaagtattattggtctccaccttttgaggtacttatgtctgaggcaaactaaaattagatcagttccaaagtccataaaaaatcttgcattcttgcaaactttagatctgagggccaccaaggtgacaaaattaccaaaaacgatctacaaattccagaatatgcgtcatcttttagcttatagctgtgatatgaaatctggagtggcacgaggagtggacgtgtctgcgggcaatattggatcgttatcttccatacagaagttgttactagtcaaggtgcaaaaaaacagaaaagccataaaagccttgagagagttgattggtctacggaagttggggctggtcgaccttggaagagaacatggaagagaattgtgttgttccattcaaaagatgtcagagctttcaacacttgtggtacgatcaacaagcgaggaggagtatctcgatttggatcatatgaattccccccctggtcctcctctctatctccaacgtctatctttgacagggcgcttaaaaattctaccgcagtggatagcgcagcttcatagtctgactaggattgctttgaagggttcaaaactgaacgctggtgccaacagtactgttgaggtccttcaagatttgcctaatctgatggaactagaattggttgactcattcactggggtatctttgatattcaaagctgaaaagttcaagaaactaaaggccttgcgtattgagaaatttgatcagctagatttgatggtcgtcgaggttggagcaatgcctcagctcaagaagttaaccatgtctaaatgccagaatctgaagttacttccatttggcattggtcatctaatcttcctcgaagaactgctcttgattgacatgcaccatgaattccttgctaggctacagaaggatagcgaagaccgttacttggttgagaatattcgagttatccattccttctctgggagctaccaatcttacactggattccaaaatctatcttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1034

Amino Acids

117.86

Weight (kDa)

9.06

Isoelectric Point (pI)

50.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2696
AccBSI CCGCTC 1 cut(s) 62
AccI GTMKAC 2 cut(s) 2425, 2445
AccII CGCG 2 cut(s) 1386, 1656
AciI CCGC 7 cut(s) 62, 65, 807, 1013, 1561, 2327, 2630
AclWI GGATC 4 cut(s) 1528, 1593, 2347, 2562
AcsI RAATTY 8 cut(s) 769, 1911, 2048, 2249, 2563, 2622, 2831, 2987
AcuI CTGAAG 3 cut(s) 1436, 1884, 2936
AfeI AGCGCT 1 cut(s) 967
AfiI CCNNNNNNNGG 6 cut(s) 112, 381, 911, 1706, 3028, 3063
AflIII ACRYGT 1 cut(s) 2319
AhlI ACTAGT 2 cut(s) 317, 2368
AjiI CACGTC 2 cut(s) 899, 2320
AjnI CCWGG 8 cut(s) 347, 802, 1234, 1319, 1331, 1699, 1778, 2572
AjuI GAANNNNNNNTTGG 2 cut(s) 597, 629
AleI CACNNNNGTG 2 cut(s) 827, 2219
Alw21I GWGCWC 2 cut(s) 1392, 1398
Alw26I GTCTC 1 cut(s) 2117
AlwI GGATC 4 cut(s) 1528, 1593, 2347, 2562
AlwNI CAGNNNCTG 1 cut(s) 2914
Ama87I CYCGRG 2 cut(s) 701, 1391
Aor51HI AGCGCT 1 cut(s) 967
AoxI GGCC 4 cut(s) 143, 345, 2211, 2814
ApeKI GCWGC 4 cut(s) 1015, 1109, 1570, 2644
ApoI RAATTY 8 cut(s) 769, 1911, 2048, 2249, 2563, 2622, 2831, 2987
ArsI GACNNNNNNTTYG 2 cut(s) 194, 226
AspLEI GCGC 3 cut(s) 968, 2616, 2644
AspS9I GGNCC 6 cut(s) 304, 439, 800, 2211, 2576, 2716
AsuHPI GGTGA 6 cut(s) 91, 107, 301, 344, 1916, 2233
AsuNHI GCTAGC 1 cut(s) 8
AvaI CYCGRG 2 cut(s) 701, 1391
AvaII GGWCC 5 cut(s) 304, 439, 800, 2576, 2716
BaeGI GKGCMC 1 cut(s) 71
BaeI ACNNNNGTAYC 6 cut(s) 1966, 1999, 2119, 2152, 2757, 2790
BanI GGYRCC 1 cut(s) 2696
BanII GRGCYC 3 cut(s) 182, 1392, 1881
BarI GAAGNNNNNNTAC 2 cut(s) 1882, 1914
BauI CACGAG 1 cut(s) 2307
BbsI GAAGAC 3 cut(s) 1080, 1644, 3024
Bbv12I GWGCWC 2 cut(s) 1392, 1398
BbvCI CCTCAGC 1 cut(s) 2877
BbvI GCAGC 4 cut(s) 1027, 1121, 1582, 2656
BccI CCATC 7 cut(s) 19, 23, 190, 1605, 1787, 2734, 2845
BciT130I CCWGG 8 cut(s) 349, 804, 1236, 1321, 1333, 1701, 1780, 2574
BclI TGATCA 1 cut(s) 2836
BcoDI GTCTC 1 cut(s) 2117
BcuI ACTAGT 2 cut(s) 317, 2368
BfmI CTRYAG 4 cut(s) 190, 264, 1885, 3002
BfoI RGCGCY 2 cut(s) 969, 2617
BglII AGATCT 2 cut(s) 1195, 2203
BisI GCNGC 7 cut(s) 63, 808, 1016, 1110, 1561, 1571, 2645
BlsI GCNGC 7 cut(s) 64, 809, 1017, 1111, 1562, 1572, 2646
BmcAI AGTACT 2 cut(s) 1891, 2707
Bme1390I CCNGG 8 cut(s) 349, 804, 1236, 1321, 1333, 1701, 1780, 2574
Bme18I GGWCC 5 cut(s) 304, 439, 800, 2576, 2716
BmeT110I CYCGRG 2 cut(s) 701, 1391
BmgBI CACGTC 2 cut(s) 899, 2320
BmgT120I GGNCC 6 cut(s) 304, 439, 800, 2211, 2576, 2716
BmiI GGNNCC 3 cut(s) 811, 1878, 2698
BmrFI CCNGG 8 cut(s) 349, 804, 1236, 1321, 1333, 1701, 1780, 2574
BmrI ACTGGG 3 cut(s) 1408, 1569, 2778
BmsI GCATC 4 cut(s) 88, 357, 457, 1686
BmtI GCTAGC 1 cut(s) 12
BmuI ACTGGG 3 cut(s) 1408, 1569, 2778
BoxI GACNNNNGTC 1 cut(s) 1971
BpiI GAAGAC 3 cut(s) 1080, 1644, 3024
BplI GAGNNNNNCTC 2 cut(s) 569, 601
BpmI CTGGAG 3 cut(s) 134, 1470, 2319
Bpu10I CCTNAGC 1 cut(s) 2877
BpuEI CTTGAG 3 cut(s) 1990, 2428, 2867
Bsa29I ATCGAT 1 cut(s) 939
BsaAI YACGTR 1 cut(s) 403
BsaBI GATNNNNATC 2 cut(s) 2344, 2553
BsaI GGTCTC 1 cut(s) 2117
BsaJI CCNNGG 7 cut(s) 630, 1235, 1516, 1848, 2217, 2449, 2572
BsaWI WCCGGW 1 cut(s) 1279
BsaXI ACNNNNNCTCC 6 cut(s) 79, 109, 1798, 1828, 2304, 2334
Bsc4I CCNNNNNNNGG 6 cut(s) 112, 381, 911, 1706, 3028, 3063
Bse1I ACTGG 8 cut(s) 117, 858, 1040, 1403, 1487, 1575, 2773, 3088
Bse3DI GCAATG 3 cut(s) 540, 1752, 2877
Bse8I GATNNNNATC 2 cut(s) 2344, 2553
BseBI CCWGG 8 cut(s) 349, 804, 1236, 1321, 1333, 1701, 1780, 2574
BseCI ATCGAT 1 cut(s) 939
BseDI CCNNGG 7 cut(s) 630, 1235, 1516, 1848, 2217, 2449, 2572
BseGI GGATG 6 cut(s) 27, 34, 205, 249, 372, 472
BseJI GATNNNNATC 2 cut(s) 2344, 2553
BseLI CCNNNNNNNGG 6 cut(s) 112, 381, 911, 1706, 3028, 3063
BseMI GCAATG 3 cut(s) 540, 1752, 2877
BseMII CTCAG 5 cut(s) 110, 516, 2129, 2198, 2891
BseNI ACTGG 8 cut(s) 117, 858, 1040, 1403, 1487, 1575, 2773, 3088
BseSI GKGCMC 1 cut(s) 71
BseXI GCAGC 4 cut(s) 1027, 1121, 1582, 2656
Bsh1236I CGCG 2 cut(s) 1386, 1656
BshFI GGCC 4 cut(s) 145, 347, 2213, 2816
BshNI GGYRCC 1 cut(s) 2696
BshVI ATCGAT 1 cut(s) 939
BsiHKAI GWGCWC 2 cut(s) 1392, 1398
BsiHKCI CYCGRG 2 cut(s) 701, 1391
BsiSI CCGG 2 cut(s) 813, 1280
BslFI GGGAC 2 cut(s) 398, 1979
BslI CCNNNNNNNGG 6 cut(s) 112, 381, 911, 1706, 3028, 3063
BsmAI GTCTC 1 cut(s) 2117
BsmFI GGGAC 2 cut(s) 398, 1979
BsmI GAATGC 1 cut(s) 2186
BsnI GGCC 4 cut(s) 145, 347, 2213, 2816
Bso31I GGTCTC 1 cut(s) 2117
BsoBI CYCGRG 2 cut(s) 701, 1391
Bsp1286I GDGCHC 5 cut(s) 71, 182, 1392, 1398, 1881
Bsp68I TCGCGA 1 cut(s) 1656
BspACI CCGC 7 cut(s) 62, 65, 807, 1013, 1561, 2327, 2630
BspANI GGCC 4 cut(s) 145, 347, 2213, 2816
BspCNI CTCAG 5 cut(s) 111, 517, 2130, 2199, 2890
BspDI ATCGAT 1 cut(s) 939
BspFNI CGCG 2 cut(s) 1386, 1656
BspHI TCATGA 1 cut(s) 1045
BspLI GGNNCC 3 cut(s) 811, 1878, 2698
BspMAI CTGCAG 1 cut(s) 1889
BspOI GCTAGC 1 cut(s) 12
BspPI GGATC 4 cut(s) 1528, 1593, 2347, 2562
BspQI GCTCTTC 4 cut(s) 143, 559, 723, 785
BspT107I GGYRCC 1 cut(s) 2696
BspTNI GGTCTC 1 cut(s) 2117
BsrBI CCGCTC 1 cut(s) 62
BsrDI GCAATG 3 cut(s) 540, 1752, 2877
BsrI ACTGG 8 cut(s) 117, 858, 1040, 1403, 1487, 1575, 2773, 3088
BssECI CCNNGG 7 cut(s) 630, 1235, 1516, 1848, 2217, 2449, 2572
BssSI CACGAG 1 cut(s) 2307
BssT1I CCWWGG 5 cut(s) 630, 1516, 1848, 2217, 2449
Bst2BI CACGAG 1 cut(s) 2307
Bst2UI CCWGG 8 cut(s) 349, 804, 1236, 1321, 1333, 1701, 1780, 2574
Bst4CI ACNGT 8 cut(s) 36, 79, 756, 848, 1870, 2705, 2710, 3023
Bst6I CTCTTC 9 cut(s) 143, 168, 387, 559, 723, 785, 1699, 2449, 2461
BstBAI YACGTR 1 cut(s) 403
BstDEI CTNAG 7 cut(s) 119, 380, 525, 1152, 2138, 2207, 2877
BstENI CCTNNNNNAGG 1 cut(s) 379
BstF5I GGATG 6 cut(s) 27, 34, 205, 249, 372, 472
BstFNI CGCG 2 cut(s) 1386, 1656
BstH2I RGCGCY 2 cut(s) 969, 2617
BstHHI GCGC 3 cut(s) 968, 2616, 2644
BstMAI GTCTC 1 cut(s) 2117
BstMWI GCNNNNNNNGC 4 cut(s) 813, 1283, 1448, 2402
BstNI CCWGG 8 cut(s) 349, 804, 1236, 1321, 1333, 1701, 1780, 2574
BstNSI RCATGY 3 cut(s) 1630, 2010, 2980
BstPAI GACNNNNGTC 1 cut(s) 1971
BstSCI CCNGG 8 cut(s) 347, 802, 1234, 1319, 1331, 1699, 1778, 2572
BstSFI CTRYAG 4 cut(s) 190, 264, 1885, 3002
BstSLI GKGCMC 1 cut(s) 71
BstSNI TACGTA 1 cut(s) 403
BstUI CGCG 2 cut(s) 1386, 1656
BstV1I GCAGC 4 cut(s) 1027, 1121, 1582, 2656
BstV2I GAAGAC 3 cut(s) 1080, 1644, 3024
BstX2I RGATCY 4 cut(s) 1195, 1520, 1585, 2203
BstXI CCANNNNNNTGG 2 cut(s) 1494, 1582
BstYI RGATCY 4 cut(s) 1195, 1520, 1585, 2203
Bsu15I ATCGAT 1 cut(s) 939
BsuRI GGCC 4 cut(s) 145, 347, 2213, 2816
BsuTUI ATCGAT 1 cut(s) 939
BtrI CACGTC 2 cut(s) 899, 2320
BtsCI GGATG 6 cut(s) 27, 34, 205, 249, 372, 472
BtsI GCAGTG 1 cut(s) 2639
BtsIMutI CAGTG 6 cut(s) 41, 1396, 1866, 2639, 2766, 3081
BtuMI TCGCGA 1 cut(s) 1656
CaiI CAGNNNCTG 1 cut(s) 2914
CciI TCATGA 1 cut(s) 1045
CfoI GCGC 3 cut(s) 968, 2616, 2644
Cfr13I GGNCC 6 cut(s) 304, 439, 800, 2211, 2576, 2716
ClaI ATCGAT 1 cut(s) 939
CseI GACGC 1 cut(s) 2252
CspCI CAANNNNNGTGG 2 cut(s) 2496, 2531
DdeI CTNAG 7 cut(s) 119, 380, 525, 1152, 2138, 2207, 2877
Eam1104I CTCTTC 9 cut(s) 143, 168, 387, 559, 723, 785, 1699, 2449, 2461
EarI CTCTTC 9 cut(s) 143, 168, 387, 559, 723, 785, 1699, 2449, 2461
Ecl136II GAGCTC 1 cut(s) 1390
Eco105I TACGTA 1 cut(s) 403
Eco130I CCWWGG 5 cut(s) 630, 1516, 1848, 2217, 2449
Eco147I AGGCCT 2 cut(s) 347, 2816
Eco24I GRGCYC 3 cut(s) 182, 1392, 1881
Eco31I GGTCTC 1 cut(s) 2117
Eco47I GGWCC 5 cut(s) 304, 439, 800, 2576, 2716
Eco47III AGCGCT 1 cut(s) 967
Eco53kI GAGCTC 1 cut(s) 1390
Eco57I CTGAAG 3 cut(s) 1436, 1884, 2936
Eco88I CYCGRG 2 cut(s) 701, 1391
EcoICRI GAGCTC 1 cut(s) 1390
EcoNI CCTNNNNNAGG 1 cut(s) 379
EcoO109I RGGNCCY 1 cut(s) 2716
EcoRI GAATTC 2 cut(s) 2563, 2987
EcoRII CCWGG 8 cut(s) 347, 802, 1234, 1319, 1331, 1699, 1778, 2572
EcoT14I CCWWGG 5 cut(s) 630, 1516, 1848, 2217, 2449
EcoT38I GRGCYC 3 cut(s) 182, 1392, 1881
ErhI CCWWGG 5 cut(s) 630, 1516, 1848, 2217, 2449
FaqI GGGAC 2 cut(s) 398, 1979
FauI CCCGC 2 cut(s) 58, 2320
FauNDI CATATG 1 cut(s) 2559
FbaI TGATCA 1 cut(s) 2836
FblI GTMKAC 2 cut(s) 2425, 2445
Fnu4HI GCNGC 7 cut(s) 63, 808, 1016, 1110, 1561, 1571, 2645
FokI GGATG 6 cut(s) 34, 41, 192, 236, 379, 479
FriOI GRGCYC 3 cut(s) 182, 1392, 1881
Fsp4HI GCNGC 7 cut(s) 63, 808, 1016, 1110, 1561, 1571, 2645
GlaI GCGC 3 cut(s) 967, 2615, 2643
GluI GCNGC 7 cut(s) 63, 808, 1016, 1110, 1561, 1571, 2645
GsuI CTGGAG 3 cut(s) 134, 1470, 2319
HaeII RGCGCY 2 cut(s) 969, 2617
HaeIII GGCC 4 cut(s) 145, 347, 2213, 2816
HapII CCGG 2 cut(s) 813, 1280
HgaI GACGC 1 cut(s) 2252
HhaI GCGC 3 cut(s) 968, 2616, 2644
Hin6I GCGC 3 cut(s) 966, 2614, 2642
HinP1I GCGC 3 cut(s) 966, 2614, 2642
HincII GTYRAC 4 cut(s) 759, 2446, 2758, 2892
HindII GTYRAC 4 cut(s) 759, 2446, 2758, 2892
HpaI GTTAAC 1 cut(s) 2892
HpaII CCGG 2 cut(s) 813, 1280
HphI GGTGA 6 cut(s) 91, 107, 301, 344, 1916, 2233
Hpy166II GTNNAC 8 cut(s) 759, 851, 1382, 2317, 2426, 2446, 2758, 2892
Hpy8I GTNNAC 8 cut(s) 759, 851, 1382, 2317, 2426, 2446, 2758, 2892
Hpy99I CGWCG 2 cut(s) 1920, 2861
HpyCH4III ACNGT 8 cut(s) 36, 79, 756, 848, 1870, 2705, 2710, 3023
HpyCH4IV ACGT 4 cut(s) 402, 898, 2319, 2596
HpyF10VI GCNNNNNNNGC 4 cut(s) 813, 1283, 1448, 2402
HpyF3I CTNAG 7 cut(s) 119, 380, 525, 1152, 2138, 2207, 2877
HpySE526I ACGT 4 cut(s) 402, 898, 2319, 2596
HspAI GCGC 3 cut(s) 966, 2614, 2642
Ksp22I TGATCA 1 cut(s) 2836
KspAI GTTAAC 1 cut(s) 2892
LguI GCTCTTC 4 cut(s) 143, 559, 723, 785
LmnI GCTCC 8 cut(s) 59, 115, 815, 1239, 1282, 1876, 2867, 3066
Lsp1109I GCAGC 4 cut(s) 1027, 1121, 1582, 2656
LweI GCATC 4 cut(s) 88, 357, 457, 1686
MaeII ACGT 4 cut(s) 402, 898, 2319, 2596
MaeIII GTNAC 7 cut(s) 894, 1503, 1973, 2221, 2364, 2919, 3023
MbiI CCGCTC 1 cut(s) 62
MflI RGATCY 4 cut(s) 1195, 1520, 1585, 2203
MhlI GDGCHC 5 cut(s) 71, 182, 1392, 1398, 1881
MlyI GAGTC 4 cut(s) 380, 1660, 1981, 2753
MmeI TCCRAC 2 cut(s) 2617, 2845
MseI TTAA 9 cut(s) 594, 935, 1646, 1770, 1820, 1955, 2037, 2618, 2891
MslI CAYNNNNRTG 3 cut(s) 333, 827, 2219
MspA1I CMGCKG 2 cut(s) 1112, 1451
MspI CCGG 2 cut(s) 813, 1280
MspR9I CCNGG 8 cut(s) 349, 804, 1236, 1321, 1333, 1701, 1780, 2574
Mva1269I GAATGC 1 cut(s) 2186
MvaI CCWGG 8 cut(s) 349, 804, 1236, 1321, 1333, 1701, 1780, 2574
MvnI CGCG 2 cut(s) 1386, 1656
MwoI GCNNNNNNNGC 4 cut(s) 813, 1283, 1448, 2402
NdeI CATATG 1 cut(s) 2559
NheI GCTAGC 1 cut(s) 8
NlaIV GGNNCC 3 cut(s) 811, 1878, 2698
NmuCI GTSAC 3 cut(s) 894, 1973, 2221
NruI TCGCGA 1 cut(s) 1656
NspI RCATGY 3 cut(s) 1630, 2010, 2980
OliI CACNNNNGTG 2 cut(s) 827, 2219
PaeR7I CTCGAG 2 cut(s) 701, 1391
PagI TCATGA 1 cut(s) 1045
PceI AGGCCT 2 cut(s) 347, 2816
PciSI GCTCTTC 4 cut(s) 143, 559, 723, 785
PctI GAATGC 1 cut(s) 2186
PfeI GAWTC 8 cut(s) 202, 493, 532, 635, 1202, 1538, 2911, 3087
PfoI TCCNGGA 1 cut(s) 1319
PkrI GCNGC 7 cut(s) 64, 809, 1017, 1111, 1562, 1572, 2646
PleI GAGTC 4 cut(s) 380, 1660, 1980, 2753
PpsI GAGTC 4 cut(s) 380, 1660, 1980, 2753
Ppu21I YACGTR 1 cut(s) 403
PpuMI RGGWCCY 1 cut(s) 2716
PshAI GACNNNNGTC 1 cut(s) 1971
Psp124BI GAGCTC 1 cut(s) 1392
Psp5II RGGWCCY 1 cut(s) 2716
Psp6I CCWGG 8 cut(s) 347, 802, 1234, 1319, 1331, 1699, 1778, 2572
PspGI CCWGG 8 cut(s) 347, 802, 1234, 1319, 1331, 1699, 1778, 2572
PspN4I GGNNCC 3 cut(s) 811, 1878, 2698
PspPI GGNCC 6 cut(s) 304, 439, 800, 2211, 2576, 2716
PspPPI RGGWCCY 1 cut(s) 2716
PspXI VCTCGAGB 1 cut(s) 1391
PstI CTGCAG 1 cut(s) 1889
PstNI CAGNNNCTG 1 cut(s) 2914
PsuI RGATCY 4 cut(s) 1195, 1520, 1585, 2203
PvuII CAGCTG 2 cut(s) 1112, 1451
RruI TCGCGA 1 cut(s) 1656
RseI CAYNNNNRTG 3 cut(s) 333, 827, 2219
SacI GAGCTC 1 cut(s) 1392
SalI GTCGAC 1 cut(s) 2444
SapI GCTCTTC 4 cut(s) 143, 559, 723, 785
SaqAI TTAA 9 cut(s) 594, 935, 1646, 1770, 1820, 1955, 2037, 2618, 2891
SatI GCNGC 7 cut(s) 63, 808, 1016, 1110, 1561, 1571, 2645
Sau96I GGNCC 6 cut(s) 304, 439, 800, 2211, 2576, 2716
ScaI AGTACT 2 cut(s) 1891, 2707
SchI GAGTC 4 cut(s) 380, 1660, 1981, 2753
ScrFI CCNGG 8 cut(s) 349, 804, 1236, 1321, 1333, 1701, 1780, 2574
SduI GDGCHC 5 cut(s) 71, 182, 1392, 1398, 1881
SfaNI GCATC 4 cut(s) 88, 357, 457, 1686
SfcI CTRYAG 4 cut(s) 190, 264, 1885, 3002
Sfr274I CTCGAG 2 cut(s) 701, 1391
SinI GGWCC 5 cut(s) 304, 439, 800, 2576, 2716
SlaI CTCGAG 2 cut(s) 701, 1391
SmiMI CAYNNNNRTG 3 cut(s) 333, 827, 2219
SmlI CTYRAG 5 cut(s) 701, 1391, 1969, 2407, 2882
SmoI CTYRAG 5 cut(s) 701, 1391, 1969, 2407, 2882
SnaBI TACGTA 1 cut(s) 403
SpeI ACTAGT 2 cut(s) 317, 2368
SseBI AGGCCT 2 cut(s) 347, 2816
SsiI CCGC 7 cut(s) 62, 65, 807, 1013, 1561, 2327, 2630
SspI AATATT 3 cut(s) 273, 2335, 3040
SstI GAGCTC 1 cut(s) 1392
StuI AGGCCT 2 cut(s) 347, 2816
StyD4I CCNGG 8 cut(s) 347, 802, 1234, 1319, 1331, 1699, 1778, 2572
StyI CCWWGG 5 cut(s) 630, 1516, 1848, 2217, 2449
TaaI ACNGT 8 cut(s) 36, 79, 756, 848, 1870, 2705, 2710, 3023
TaiI ACGT 4 cut(s) 405, 901, 2322, 2599
TatI WGTACW 3 cut(s) 1189, 1889, 2705
TauI GCSGC 3 cut(s) 65, 810, 1563
TfiI GAWTC 8 cut(s) 202, 493, 532, 635, 1202, 1538, 2911, 3087
Tru1I TTAA 9 cut(s) 594, 935, 1646, 1770, 1820, 1955, 2037, 2618, 2891
Tru9I TTAA 9 cut(s) 594, 935, 1646, 1770, 1820, 1955, 2037, 2618, 2891
TscAI CASTG 6 cut(s) 41, 1403, 1873, 2639, 2773, 3088
TseFI GTSAC 3 cut(s) 894, 1973, 2221
TseI GCWGC 4 cut(s) 1015, 1109, 1570, 2644
Tsp45I GTSAC 3 cut(s) 894, 1973, 2221
TspGWI ACGGA 4 cut(s) 198, 247, 1276, 2443
TspRI CASTG 6 cut(s) 41, 1403, 1873, 2639, 2773, 3088
VpaK11BI GGWCC 5 cut(s) 304, 439, 800, 2576, 2716
XagI CCTNNNNNAGG 1 cut(s) 379
XapI RAATTY 8 cut(s) 769, 1911, 2048, 2249, 2563, 2622, 2831, 2987
XceI RCATGY 3 cut(s) 1630, 2010, 2980
XcmI CCANNNNNNNNNTGG 2 cut(s) 3059, 3080
XhoI CTCGAG 2 cut(s) 701, 1391
XmiI GTMKAC 2 cut(s) 2425, 2445
ZrmI AGTACT 2 cut(s) 1891, 2707
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.