RchiOBHm_Chr2g0127041

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000865
Physical Location & Seq
Reverse (-)
41771291 .. 41772160
870 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49894

Sequence Viewer

Length: 870 bp
ATGTCACAGAAGTTTGTCACTGATTCCGAAACAATCTGGTGCAGACAAACAGAAGTTGACAAAGTTCGACGCCTCTCTGCTCCTAATCTGATTATCAAATATCTGTCAAACTCTGGAATTGACTTGAATGGTATTCGTACATTACAGAGTTACAAGAAGGGAAGTTCTGGACTTGACTTGAATGTTATTCGTACCTTACAAGGTCACAAGAAGGAAAATTCTGATTCTCAGTTTGCCATGGTACTTAAAACCACTAGATTTTTAAGGGTATTAGATTTGCAAGGTGTACCTTTGGAAAAATTTCCTTCTAGTGTTGTTGGCCTCACCCTCTTAAAGTATCTAAGTCTAAGGAAATCCCAGATCAAAACAGTTCCCCAGTCCATAGAAAAGTTGTTATTCTTAGAAACCTTGGATCTTAAATACACCCGGGTGACCCATTTGCCAGAAGAGATTTGTAAACTTGTAAGGTTGCGCCATCTATTAGTATGCAATAATCCCGGTAGTGGAGGAGTGACTCTTTCTGCAGGCAATCTTGGAGCCCTATCTTCCATACAAAATTTGTCATTAGTTAAGGTGGAAAACGACAGAAGAATCCTAAAAGCCTTGGGAAAGCTAATAGGTCTCAGAAAGTTGGGGCTGACAGATGTGAGAAAAGAAGATGGAGTAGAATTGTGTTGTTCTCTTCAGAAAATGGAGAATCTTTCATCATTTGATGTAGCATCAACAAGCAAGGATGAGTATCTTGATTTGGATCACATGCAATCTCCTCTAGTGGCTCCTCTCTATCTTGAACGTTTATTTTTGATAGGAAGGCTAGAAAGGGTACCTAAGTGGATTCCTGAGCTTACGAGTCTAACAAAGATTCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

289

Amino Acids

32.5

Weight (kDa)

9.5

Isoelectric Point (pI)

43.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 78 - 286 1.2e-32 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 823
AccB1I GGYRCC 1 cut(s) 823
AclI AACGTT 1 cut(s) 793
AclWI GGATC 2 cut(s) 420, 759
AcsI RAATTY 3 cut(s) 217, 299, 556
AcuI CTGAAG 1 cut(s) 668
AcyI GRCGYC 1 cut(s) 70
AfaI GTAC 5 cut(s) 139, 193, 243, 288, 825
AfiI CCNNNNNNNGG 1 cut(s) 503
AgsI TTSAA 3 cut(s) 127, 181, 791
AleI CACNNNNGTG 1 cut(s) 428
AluBI AGCT 2 cut(s) 613, 844
AluI AGCT 2 cut(s) 613, 844
Alw26I GTCTC 1 cut(s) 626
AlwI GGATC 2 cut(s) 420, 759
Ama87I CYCGRG 1 cut(s) 426
AoxI GGCC 1 cut(s) 319
ApoI RAATTY 3 cut(s) 217, 299, 556
Asp700I GAANNNNTTC 1 cut(s) 300
Asp718I GGTACC 1 cut(s) 823
AspLEI GCGC 1 cut(s) 474
AsuC2I CCSGG 3 cut(s) 427, 428, 498
AsuHPI GGTGA 2 cut(s) 316, 442
AvaI CYCGRG 1 cut(s) 426
BaeI ACNNNNGTAYC 2 cut(s) 815, 848
BanI GGYRCC 1 cut(s) 823
BanII GRGCYC 1 cut(s) 541
BccI CCATC 2 cut(s) 483, 653
BcnI CCSGG 3 cut(s) 427, 428, 498
BcoDI GTCTC 1 cut(s) 626
BfaI CTAG 4 cut(s) 255, 309, 770, 815
BfmI CTRYAG 1 cut(s) 522
Bme1390I CCNGG 3 cut(s) 427, 428, 498
BmeT110I CYCGRG 1 cut(s) 426
BmiI GGNNCC 3 cut(s) 538, 777, 825
BmrFI CCNGG 3 cut(s) 427, 428, 498
BmrI ACTGGG 1 cut(s) 370
BmsI GCATC 1 cut(s) 728
BmuI ACTGGG 1 cut(s) 370
Bpu10I CCTNAGC 1 cut(s) 840
BpuMI CCSGG 3 cut(s) 427, 428, 498
BsaBI GATNNNNATC 2 cut(s) 738, 750
BsaHI GRCGYC 1 cut(s) 70
BsaI GGTCTC 1 cut(s) 626
BsaJI CCNNGG 4 cut(s) 237, 408, 426, 603
Bsc4I CCNNNNNNNGG 1 cut(s) 503
Bse1I ACTGG 1 cut(s) 376
Bse8I GATNNNNATC 2 cut(s) 738, 750
BseDI CCNNGG 4 cut(s) 237, 408, 426, 603
BseGI GGATG 1 cut(s) 739
BseJI GATNNNNATC 2 cut(s) 738, 750
BseLI CCNNNNNNNGG 1 cut(s) 503
BseMII CTCAG 3 cut(s) 242, 637, 831
BseNI ACTGG 1 cut(s) 376
BseRI GAGGAG 3 cut(s) 522, 756, 768
BsgI GTGCAG 1 cut(s) 61
BshFI GGCC 1 cut(s) 321
BshNI GGYRCC 1 cut(s) 823
BsiHKCI CYCGRG 1 cut(s) 426
BsiSI CCGG 2 cut(s) 427, 498
BslI CCNNNNNNNGG 1 cut(s) 503
BsmAI GTCTC 1 cut(s) 626
BsnI GGCC 1 cut(s) 321
Bso31I GGTCTC 1 cut(s) 626
BsoBI CYCGRG 1 cut(s) 426
Bsp1286I GDGCHC 1 cut(s) 541
Bsp143I GATC 3 cut(s) 360, 412, 751
Bsp19I CCATGG 1 cut(s) 237
BspANI GGCC 1 cut(s) 321
BspCNI CTCAG 3 cut(s) 241, 636, 832
BspLI GGNNCC 3 cut(s) 538, 777, 825
BspMAI CTGCAG 1 cut(s) 526
BspPI GGATC 2 cut(s) 420, 759
BspT107I GGYRCC 1 cut(s) 823
BspTNI GGTCTC 1 cut(s) 626
BsrI ACTGG 1 cut(s) 376
BssECI CCNNGG 4 cut(s) 237, 408, 426, 603
BssMI GATC 3 cut(s) 360, 412, 751
BssNI GRCGYC 1 cut(s) 70
BssT1I CCWWGG 3 cut(s) 237, 408, 603
Bst4CI ACNGT 1 cut(s) 370
Bst6I CTCTTC 2 cut(s) 441, 687
BstACI GRCGYC 1 cut(s) 70
BstC8I GCNNGC 1 cut(s) 526
BstDEI CTNAG 7 cut(s) 228, 341, 347, 400, 623, 828, 840
BstDSI CCRYGG 1 cut(s) 237
BstEII GGTNACC 1 cut(s) 430
BstF5I GGATG 1 cut(s) 739
BstHHI GCGC 1 cut(s) 474
BstKTI GATC 3 cut(s) 363, 415, 754
BstMAI GTCTC 1 cut(s) 626
BstMBI GATC 3 cut(s) 360, 412, 751
BstNSI RCATGY 1 cut(s) 760
BstPI GGTNACC 1 cut(s) 430
BstSCI CCNGG 3 cut(s) 425, 426, 496
BstSFI CTRYAG 1 cut(s) 522
BstX2I RGATCY 1 cut(s) 412
BstYI RGATCY 1 cut(s) 412
BsuRI GGCC 1 cut(s) 321
BtgI CCRYGG 1 cut(s) 237
BtsCI GGATG 1 cut(s) 739
BtsIMutI CAGTG 1 cut(s) 18
Cac8I GCNNGC 1 cut(s) 526
CfoI GCGC 1 cut(s) 474
Cfr9I CCCGGG 1 cut(s) 426
CseI GACGC 1 cut(s) 78
Csp6I GTAC 5 cut(s) 138, 192, 242, 287, 824
CviAII CATG 2 cut(s) 238, 757
CviJI RGCY 8 cut(s) 321, 539, 602, 613, 637, 776, 814, 844
CviKI_1 RGCY 8 cut(s) 321, 539, 602, 613, 637, 776, 814, 844
CviQI GTAC 5 cut(s) 138, 192, 242, 287, 824
DdeI CTNAG 7 cut(s) 228, 341, 347, 400, 623, 828, 840
DpnI GATC 3 cut(s) 362, 414, 753
DpnII GATC 3 cut(s) 360, 412, 751
Eam1104I CTCTTC 2 cut(s) 441, 687
EarI CTCTTC 2 cut(s) 441, 687
Eco130I CCWWGG 3 cut(s) 237, 408, 603
Eco24I GRGCYC 1 cut(s) 541
Eco31I GGTCTC 1 cut(s) 626
Eco57I CTGAAG 1 cut(s) 668
Eco88I CYCGRG 1 cut(s) 426
Eco91I GGTNACC 1 cut(s) 430
EcoO65I GGTNACC 1 cut(s) 430
EcoT14I CCWWGG 3 cut(s) 237, 408, 603
EcoT38I GRGCYC 1 cut(s) 541
ErhI CCWWGG 3 cut(s) 237, 408, 603
FaeI CATG 2 cut(s) 241, 760
FaiI YATR 5 cut(s) 239, 383, 487, 551, 758
FatI CATG 2 cut(s) 237, 756
FokI GGATG 1 cut(s) 746
FriOI GRGCYC 1 cut(s) 541
FspBI CTAG 4 cut(s) 255, 309, 770, 815
GlaI GCGC 1 cut(s) 473
HaeIII GGCC 1 cut(s) 321
HapII CCGG 2 cut(s) 427, 498
HgaI GACGC 1 cut(s) 78
HhaI GCGC 1 cut(s) 474
Hin1I GRCGYC 1 cut(s) 70
Hin1II CATG 2 cut(s) 241, 760
Hin6I GCGC 1 cut(s) 472
HinP1I GCGC 1 cut(s) 472
HincII GTYRAC 1 cut(s) 58
HindII GTYRAC 1 cut(s) 58
HinfI GANTC 8 cut(s) 23, 224, 514, 591, 697, 835, 850, 862
HpaII CCGG 2 cut(s) 427, 498
HphI GGTGA 2 cut(s) 316, 442
Hpy166II GTNNAC 3 cut(s) 58, 287, 458
Hpy188I TCNGA 5 cut(s) 28, 90, 223, 626, 687
Hpy188III TCNNGA 5 cut(s) 114, 168, 743, 788, 839
Hpy8I GTNNAC 3 cut(s) 58, 287, 458
Hpy99I CGWCG 1 cut(s) 72
HpyAV CCTTC 4 cut(s) 151, 205, 315, 804
HpyCH4III ACNGT 1 cut(s) 370
HpyCH4IV ACGT 1 cut(s) 793
HpyCH4V TGCA 5 cut(s) 42, 280, 489, 524, 760
HpyF3I CTNAG 7 cut(s) 228, 341, 347, 400, 623, 828, 840
HpySE526I ACGT 1 cut(s) 793
Hsp92I GRCGYC 1 cut(s) 70
Hsp92II CATG 2 cut(s) 241, 760
HspAI GCGC 1 cut(s) 472
KpnI GGTACC 1 cut(s) 827
Kzo9I GATC 3 cut(s) 360, 412, 751
LmnI GCTCC 3 cut(s) 85, 536, 781
LweI GCATC 1 cut(s) 728
MaeI CTAG 4 cut(s) 255, 309, 770, 815
MaeII ACGT 1 cut(s) 793
MaeIII GTNAC 6 cut(s) 3, 16, 149, 203, 430, 511
MalI GATC 3 cut(s) 362, 414, 753
MboI GATC 3 cut(s) 360, 412, 751
MboII GAAGA 5 cut(s) 458, 537, 600, 668, 674
MflI RGATCY 1 cut(s) 412
MhlI GDGCHC 1 cut(s) 541
MluCI AATT 5 cut(s) 117, 217, 299, 556, 668
MlyI GAGTC 2 cut(s) 508, 859
MnlI CCTC 6 cut(s) 83, 332, 338, 500, 777, 789
MroXI GAANNNNTTC 1 cut(s) 300
MseI TTAA 6 cut(s) 246, 263, 332, 417, 570, 868
MslI CAYNNNNRTG 1 cut(s) 428
MspI CCGG 2 cut(s) 427, 498
MspR9I CCNGG 3 cut(s) 427, 428, 498
NciI CCSGG 3 cut(s) 427, 428, 498
NcoI CCATGG 1 cut(s) 237
NdeII GATC 3 cut(s) 360, 412, 751
NlaIII CATG 2 cut(s) 241, 760
NlaIV GGNNCC 3 cut(s) 538, 777, 825
NmuCI GTSAC 5 cut(s) 3, 16, 203, 430, 511
NspI RCATGY 1 cut(s) 760
OliI CACNNNNGTG 1 cut(s) 428
PdmI GAANNNNTTC 1 cut(s) 300
PfeI GAWTC 6 cut(s) 23, 224, 591, 697, 835, 862
PleI GAGTC 2 cut(s) 508, 858
PpsI GAGTC 2 cut(s) 508, 858
Psp1406I AACGTT 1 cut(s) 793
PspEI GGTNACC 1 cut(s) 430
PspN4I GGNNCC 3 cut(s) 538, 777, 825
PstI CTGCAG 1 cut(s) 526
PsuI RGATCY 1 cut(s) 412
RsaI GTAC 5 cut(s) 139, 193, 243, 288, 825
RsaNI GTAC 5 cut(s) 138, 192, 242, 287, 824
RseI CAYNNNNRTG 1 cut(s) 428
SaqAI TTAA 6 cut(s) 246, 263, 332, 417, 570, 868
Sau3AI GATC 3 cut(s) 360, 412, 751
SchI GAGTC 2 cut(s) 508, 859
ScrFI CCNGG 3 cut(s) 427, 428, 498
SduI GDGCHC 1 cut(s) 541
SfaNI GCATC 1 cut(s) 728
SfcI CTRYAG 1 cut(s) 522
SmaI CCCGGG 1 cut(s) 428
SmiMI CAYNNNNRTG 1 cut(s) 428
Sse9I AATT 5 cut(s) 117, 217, 299, 556, 668
SspMI CTAG 4 cut(s) 255, 309, 770, 815
StyD4I CCNGG 3 cut(s) 425, 426, 496
StyI CCWWGG 3 cut(s) 237, 408, 603
TaaI ACNGT 1 cut(s) 370
TaiI ACGT 1 cut(s) 796
TaqI TCGA 1 cut(s) 67
TasI AATT 5 cut(s) 117, 217, 299, 556, 668
TfiI GAWTC 6 cut(s) 23, 224, 591, 697, 835, 862
Tru1I TTAA 6 cut(s) 246, 263, 332, 417, 570, 868
Tru9I TTAA 6 cut(s) 246, 263, 332, 417, 570, 868
TscAI CASTG 1 cut(s) 25
TseFI GTSAC 5 cut(s) 3, 16, 203, 430, 511
Tsp45I GTSAC 5 cut(s) 3, 16, 203, 430, 511
TspDTI ATGAA 1 cut(s) 693
TspMI CCCGGG 1 cut(s) 426
TspRI CASTG 1 cut(s) 25
XapI RAATTY 3 cut(s) 217, 299, 556
XceI RCATGY 1 cut(s) 760
XmaI CCCGGG 1 cut(s) 426
XmnI GAANNNNTTC 1 cut(s) 300
XspI CTAG 4 cut(s) 255, 309, 770, 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.