Rw7G030110

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000865
Physical Location & Seq
Forward (+)
42482202 .. 42485385
3184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G030110.1

Sequence Viewer

Length: 2754 bp
ATGGCTGATGCTATTTCAGCCGCATTGCTGTTAGTTGAGACGGCGATAATCTCCACCGACCAAATACACAGTCGTACGAGTAGTGTCCGTAAGGATCTTGAAAGGGCCCGGAATTGTTTGGCGAGGTTGCAACAGTGGGATGCAGGCAACTCAAATCCCCAAGCTAGGGACAACCAGGTCCAAGATTTGGCTTATAATATTGAGGATGCTATTAACGAGTACATGCTTCAGGTTCCACAGCATTACCATACCCATCCGGTCTCTAAGCTGGCGCACAAAATTTTTCATTTCATCCCCGACCAGAAAGCAAGCAGTAACTTATCCTCCAGCATTGATGATCTGGAACAAAACCTGAAACTTCTTACGTTACTAGATTCAGTACATTCAGATGGTTCCCAAGAAGGCCGCCCAAGCTCCAGCACAGGACCTCAGGGGGGGACTTTGACCCACACAGTTCTTGAAGACCATGAAATGGTGGGCTATGATGAAGAGAAAAAACGACTTTTCCAACACTTGGGAGAAACAAATCCAGAGCTTTTGACAATTGTGATTGTAGGTCCTGGAGGCTCCGGCAAGACTACACTCGTAAAGAATGTTTACCGAAGCCAAGGGGTCCGCAGCTTGTTTGATTTTCATGTTTGGATTGATGTGCCACCCAATGTTGACCAACTCCTGCCGAAGTTGTTAAGAAGTTTTGAGCCCCAAAGGCAGGAAGTAGTTCCTCATGAAGATATCAGTGATGATCAAGCAAAACTGAGACAAATTTTGAGGGATAAGAAGTTTTTGGTTGTCCTGGATAATGTTTGGAGCAACGAAGCTTTCAAGTCCATTGTAAATGCACTACCAAAAGCCGGGGGGAGTAAACTAATCGGAATTACTAGGAATATTGATGTAGCTCCGGTTTCCTCCAGGTATACGTATACCCATAATTTGAGTGGGTTATCACCGAAGAAAGCCAACCAGCTATTCTGCGAGAAGGCTTTTCCTGATGTTGAAGGAAATCTTTGTCCAGCCGATCTGGAGGACTGGGCTGAAAAGATCATAAAGAGGTGTGAAGGCCTTCCCTGGGCAATTTCACAAATCGGCACTTTGCTAGCAAGGACACCGAGAATAGCAGTTGAGTGGAAGAAGATGCATGATACACTTGGATCCGATCTCTCCGTTGCTAGTATTTCAAGTTACAGGGATCTGCCAAGCAATATAATACAATGTTTCTTGTACTTCGGCATGTTTCCAGAAGACGAAACTATCAGCCGAGAGAGACTAATTCGGTTGTGGGTAGCCGAAGGATTTGTGAGGCCAGACGACAGAGTGAGAACGGTGGAGGCGGTTGCGGAGAGTATTCTAACTGATCTCATTAACAGAAATTTGGTTGTGGTCAGTGCGAGGGATATCGATGGACGAGTCAAGAATTGTCGTGTTTCCAACATTGTTCGCGAGTTCATCATTTCTAATGCAACGCATTTCATCACTGTTTTGGAAGCCAATAGTGTTCAAAATATTGTTCGGAATTCAAGTCAGAAAATTCGACGCCTTTCAGTTCAGTGTGATATCAATTTGTTAAGGGGTATAAACAAGAGTCGTATCACTCATACTCGTAGCTTACTAGTTTTCCGGCAGACAGCCAATAGGCCCGAGCTTGATATGGTACTTAAAGCCTGTAAGCACTTACAGGTCTTAGGTTTGCAAGGTGTACCTGATTTGGTGCACTTTCCGGAATCTGTAATTAGTCTCGCCCTGTTAAGGTACGTGAGTCTCAGGCAAACCAAAATTAAAAATGTTCCAAAATCCATAAAGAAGCTTGGAGTGTTGGAAACATTAGAACTCAAAGACACCCTGGTGACGAATTTGCCAAAAGGGATTGCTAAACTCCACCATTTGCGCCACCTTTTGGTGTATCGCAATGCTGTCAACAATAATATGACGTTTGGAGCTGCGCAAGGAGTGGAGTTTTCTACTAGCAGAGTAAGTGCTTTATCTATTTCGAATTCGTTACAAAAATTGTCTTTGATTAAGATAAACACGAACCAAAAATTCCTGAAAGCCTTAGAAGAGTTGAAAGATCTTAGAAGGTTGGGGCTGGTAGATCTCAATGGAGAAAATGGAAAAGACTTGTGTAACGCCATTCAGAAAATGGAGAATCTTTCAACATTGGATGTAAGAGCAACAAGCGAGGATGAGTATCTTGAATTGGATCATATGCAACCCGGATCCCCCCCTCTCCTTCTCCAACATCTATATTTAAAAGGGCGGCTAAAAAGGGTACCACAGTGGATTCCGGACCTTACCAGTCTCAAAAAGATTGGTTTGAAGTGGTCAAAGATGGATGCTGAGCCACTTGAGGCGCTTCAAGATTTGCCCAACCTCATGGAGCTCGATTTGGTTGACTATTACACTGGCCAGGAGTTGTGTTTCGGAGTTGAAACTTTCAAGAAACTGGTGGCATTAAGTATAGACAAATTTGATAAGCTACATTTGATCTCAATACGTAAGAAGGCAATGCCTATGCTCAAGAAGCTAACCATCTCCAGATGTCAGAATTTGAAGTTACTTCCAGTGTTTTTAAGTGGTGATCTTGAATTGCTGGAAGAATTGCTTCTATTTGACATGCCCAATGAATTCATTGCTGAGTTTGAAACATACAGCGAACGACGAGAGGATTTAAGGCATGTTGGAGTTATCCATTATTCTCGGCATCCAGGAAGCAACCAATCTGATGGATTCAAAGATCTTTCTCCATCAAAATGGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

917

Amino Acids

103.67

Weight (kDa)

8.28

Isoelectric Point (pI)

39.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 163 - 329 2e-27 NB-ARC domain
WHD_DRP PF23559 410 - 482 1.1e-16 Disease resistance protein Winged helix domain
LRR_14 PF23598 531 - 846 9.1e-39 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 195
AasI GACNNNNNNGTC 1 cut(s) 176
Acc16I TGCGCA 1 cut(s) 1938
Acc65I GGTACC 1 cut(s) 2263
AccB1I GGYRCC 1 cut(s) 2263
AccB7I CCANNNNNTGG 4 cut(s) 187, 472, 514, 1891
AccI GTMKAC 2 cut(s) 914, 920
AccII CGCG 1 cut(s) 1437
AccIII TCCGGA 2 cut(s) 1714, 2278
AciI CCGC 6 cut(s) 21, 406, 616, 1328, 1334, 2251
AclWI GGATC 7 cut(s) 102, 1143, 1156, 1194, 2202, 2205, 2218
AcoI YGGCCR 1 cut(s) 2398
AcuI CTGAAG 1 cut(s) 212
AcyI GRCGYC 1 cut(s) 1531
AfaI GTAC 8 cut(s) 76, 221, 381, 1220, 1650, 1695, 1748, 2265
AhlI ACTAGT 1 cut(s) 1606
AjnI CCWGG 8 cut(s) 174, 559, 792, 908, 1064, 1836, 2400, 2698
AjuI GAANNNNNNNTTGG 2 cut(s) 950, 982
AleI CACNNNNGTG 1 cut(s) 1838
Alw21I GWGCWC 2 cut(s) 1710, 2376
Alw26I GTCTC 7 cut(s) 32, 265, 751, 1255, 1736, 1760, 2297
Alw44I GTGCAC 1 cut(s) 1706
AlwI GGATC 7 cut(s) 102, 1143, 1156, 1194, 2202, 2205, 2218
Ama87I CYCGRG 1 cut(s) 1634
Aor13HI TCCGGA 2 cut(s) 1714, 2278
AoxI GGCC 6 cut(s) 105, 403, 1057, 1298, 1631, 2398
ApaI GGGCCC 1 cut(s) 109
ApaLI GTGCAC 1 cut(s) 1706
ApeKI GCWGC 2 cut(s) 618, 1934
ArsI GACNNNNNNTTYG 2 cut(s) 55, 87
Asp700I GAANNNNTTC 3 cut(s) 717, 1059, 2595
Asp718I GGTACC 1 cut(s) 2263
AspLEI GCGC 4 cut(s) 274, 1884, 1939, 2347
AspS9I GGNCC 8 cut(s) 105, 106, 178, 425, 557, 613, 1632, 2281
AsuC2I CCSGG 3 cut(s) 109, 853, 2208
AsuHPI GGTGA 3 cut(s) 936, 1852, 2582
AsuII TTCGAA 1 cut(s) 1985
AsuNHI GCTAGC 1 cut(s) 1093
AvaI CYCGRG 1 cut(s) 1634
AvaII GGWCC 5 cut(s) 178, 425, 557, 613, 2281
AxyI CCTNAGG 1 cut(s) 429
BaeGI GKGCMC 2 cut(s) 109, 1710
BaeI ACNNNNGTAYC 4 cut(s) 1738, 1771, 2255, 2288
BalI TGGCCA 1 cut(s) 2400
BamHI GGATCC 2 cut(s) 1148, 2210
BanI GGYRCC 1 cut(s) 2263
BanII GRGCYC 3 cut(s) 109, 702, 2376
BbsI GAAGAC 2 cut(s) 468, 1245
Bbv12I GWGCWC 2 cut(s) 1710, 2376
BbvI GCAGC 2 cut(s) 630, 1921
BccI CCATC 7 cut(s) 261, 383, 1391, 2317, 2531, 2711, 2746
BceAI ACGGC 1 cut(s) 57
BciT130I CCWGG 8 cut(s) 176, 561, 794, 910, 1066, 1838, 2402, 2700
BclI TGATCA 1 cut(s) 742
BcnI CCSGG 3 cut(s) 109, 853, 2208
BcoDI GTCTC 7 cut(s) 32, 265, 751, 1255, 1736, 1760, 2297
BcuI ACTAGT 1 cut(s) 1606
BfaI CTAG 7 cut(s) 165, 371, 879, 1094, 1167, 1607, 1959
BfoI RGCGCY 1 cut(s) 2348
BglI GCCNNNNNGGC 1 cut(s) 706
BglII AGATCT 3 cut(s) 2062, 2086, 2728
BisI GCNGC 5 cut(s) 21, 406, 619, 1935, 2252
BlpI GCTNAGC 1 cut(s) 2331
BlsI GCNGC 5 cut(s) 22, 407, 620, 1936, 2253
Bme18I GGWCC 5 cut(s) 178, 425, 557, 613, 2281
BmeT110I CYCGRG 1 cut(s) 1634
BmgT120I GGNCC 8 cut(s) 105, 106, 178, 425, 557, 613, 1632, 2281
BmiI GGNNCC 8 cut(s) 107, 234, 394, 568, 614, 1150, 2212, 2265
BmrI ACTGGG 1 cut(s) 1036
BmsI GCATC 5 cut(s) 130, 196, 1122, 2317, 2704
BmtI GCTAGC 1 cut(s) 1097
BmuI ACTGGG 1 cut(s) 1036
BpiI GAAGAC 2 cut(s) 468, 1245
BpmI CTGGAG 6 cut(s) 310, 400, 582, 892, 1040, 2512
Bpu1102I GCTNAGC 1 cut(s) 2331
Bpu14I TTCGAA 1 cut(s) 1985
BpuEI CTTGAG 2 cut(s) 2360, 2495
BpuMI CCSGG 3 cut(s) 109, 853, 2208
Bsa29I ATCGAT 1 cut(s) 1395
BsaAI YACGTR 3 cut(s) 918, 1750, 2489
BsaBI GATNNNNATC 1 cut(s) 2181
BsaHI GRCGYC 1 cut(s) 1531
BsaI GGTCTC 1 cut(s) 265
BsaJI CCNNGG 5 cut(s) 607, 852, 1064, 1065, 1836
BsaWI WCCGGW 4 cut(s) 256, 898, 1714, 2278
BsaXI ACNNNNNCTCC 2 cut(s) 308, 338
Bse1I ACTGG 5 cut(s) 1031, 2289, 2401, 2442, 2555
Bse21I CCTNAGG 1 cut(s) 429
Bse3DI GCAATG 4 cut(s) 23, 1909, 2505, 2622
Bse8I GATNNNNATC 1 cut(s) 2181
BseAI TCCGGA 2 cut(s) 1714, 2278
BseBI CCWGG 8 cut(s) 176, 561, 794, 910, 1066, 1838, 2402, 2700
BseCI ATCGAT 1 cut(s) 1395
BseDI CCNNGG 5 cut(s) 607, 852, 1064, 1065, 1836
BseGI GGATG 8 cut(s) 145, 211, 253, 291, 2161, 2182, 2332, 2695
BseJI GATNNNNATC 1 cut(s) 2181
BseMI GCAATG 4 cut(s) 23, 1909, 2505, 2622
BseMII CTCAG 5 cut(s) 443, 746, 1771, 2322, 2619
BseNI ACTGG 5 cut(s) 1031, 2289, 2401, 2442, 2555
BseSI GKGCMC 2 cut(s) 109, 1710
BseXI GCAGC 2 cut(s) 630, 1921
Bsh1236I CGCG 1 cut(s) 1437
BshFI GGCC 6 cut(s) 107, 405, 1059, 1300, 1633, 2400
BshNI GGYRCC 1 cut(s) 2263
BshVI ATCGAT 1 cut(s) 1395
BsiHKAI GWGCWC 2 cut(s) 1710, 2376
BsiHKCI CYCGRG 1 cut(s) 1634
BsiSI CCGG 9 cut(s) 109, 257, 570, 852, 899, 1615, 1715, 2208, 2279
BsiWI CGTACG 1 cut(s) 74
BslFI GGGAC 2 cut(s) 182, 451
BsmAI GTCTC 7 cut(s) 32, 265, 751, 1255, 1736, 1760, 2297
BsmBI CGTCTC 1 cut(s) 32
BsmFI GGGAC 2 cut(s) 182, 451
BsnI GGCC 6 cut(s) 107, 405, 1059, 1300, 1633, 2400
Bso31I GGTCTC 1 cut(s) 265
BsoBI CYCGRG 1 cut(s) 1634
Bsp119I TTCGAA 1 cut(s) 1985
Bsp120I GGGCCC 1 cut(s) 105
Bsp1286I GDGCHC 4 cut(s) 109, 702, 1710, 2376
Bsp13I TCCGGA 2 cut(s) 1714, 2278
Bsp1720I GCTNAGC 1 cut(s) 2331
Bsp68I TCGCGA 1 cut(s) 1437
BspACI CCGC 6 cut(s) 21, 406, 616, 1328, 1334, 2251
BspANI GGCC 6 cut(s) 107, 405, 1059, 1300, 1633, 2400
BspCNI CTCAG 5 cut(s) 442, 747, 1770, 2323, 2620
BspDI ATCGAT 1 cut(s) 1395
BspEI TCCGGA 2 cut(s) 1714, 2278
BspFNI CGCG 1 cut(s) 1437
BspHI TCATGA 1 cut(s) 724
BspLI GGNNCC 8 cut(s) 107, 234, 394, 568, 614, 1150, 2212, 2265
BspOI GCTAGC 1 cut(s) 1097
BspPI GGATC 7 cut(s) 102, 1143, 1156, 1194, 2202, 2205, 2218
BspT104I TTCGAA 1 cut(s) 1985
BspT107I GGYRCC 1 cut(s) 2263
BspTNI GGTCTC 1 cut(s) 265
BsrDI GCAATG 4 cut(s) 23, 1909, 2505, 2622
BsrI ACTGG 5 cut(s) 1031, 2289, 2401, 2442, 2555
BssECI CCNNGG 5 cut(s) 607, 852, 1064, 1065, 1836
BssNAI GTATAC 2 cut(s) 915, 921
BssNI GRCGYC 1 cut(s) 1531
BssT1I CCWWGG 1 cut(s) 607
Bst1107I GTATAC 2 cut(s) 915, 921
Bst2UI CCWGG 8 cut(s) 176, 561, 794, 910, 1066, 1838, 2402, 2700
Bst4CI ACNGT 6 cut(s) 71, 135, 454, 1321, 1474, 2271
Bst6I CTCTTC 2 cut(s) 483, 2046
BstACI GRCGYC 1 cut(s) 1531
BstBAI YACGTR 3 cut(s) 918, 1750, 2489
BstBI TTCGAA 1 cut(s) 1985
BstC8I GCNNGC 4 cut(s) 145, 270, 310, 1095
BstDEI CTNAG 9 cut(s) 264, 429, 755, 1678, 1757, 2047, 2066, 2331, 2628
BstF5I GGATG 8 cut(s) 145, 211, 253, 291, 2161, 2182, 2332, 2695
BstFNI CGCG 1 cut(s) 1437
BstH2I RGCGCY 1 cut(s) 2348
BstHHI GCGC 4 cut(s) 274, 1884, 1939, 2347
BstMAI GTCTC 7 cut(s) 32, 265, 751, 1255, 1736, 1760, 2297
BstMWI GCNNNNNNNGC 4 cut(s) 17, 411, 706, 2515
BstNI CCWGG 8 cut(s) 176, 561, 794, 910, 1066, 1838, 2402, 2700
BstNSI RCATGY 4 cut(s) 226, 1231, 2611, 2672
BstSLI GKGCMC 2 cut(s) 109, 1710
BstSNI TACGTA 2 cut(s) 918, 2489
BstUI CGCG 1 cut(s) 1437
BstV1I GCAGC 2 cut(s) 630, 1921
BstV2I GAAGAC 2 cut(s) 468, 1245
BstX2I RGATCY 7 cut(s) 94, 1148, 1186, 2062, 2086, 2210, 2728
BstXI CCANNNNNNTGG 3 cut(s) 2368, 2717, 2745
BstYI RGATCY 7 cut(s) 94, 1148, 1186, 2062, 2086, 2210, 2728
BstZ17I GTATAC 2 cut(s) 915, 921
Bsu15I ATCGAT 1 cut(s) 1395
Bsu36I CCTNAGG 1 cut(s) 429
BsuRI GGCC 6 cut(s) 107, 405, 1059, 1300, 1633, 2400
BsuTUI ATCGAT 1 cut(s) 1395
BtsCI GGATG 8 cut(s) 145, 211, 253, 291, 2161, 2182, 2332, 2695
BtsIMutI CAGTG 8 cut(s) 140, 742, 1387, 1470, 1550, 2276, 2394, 2562
BtuMI TCGCGA 1 cut(s) 1437
Cac8I GCNNGC 4 cut(s) 145, 270, 310, 1095
CciI TCATGA 1 cut(s) 724
CfoI GCGC 4 cut(s) 274, 1884, 1939, 2347
Cfr13I GGNCC 8 cut(s) 105, 106, 178, 425, 557, 613, 1632, 2281
ClaI ATCGAT 1 cut(s) 1395
CseI GACGC 1 cut(s) 1539
CsiI ACCWGGT 1 cut(s) 174
Csp6I GTAC 8 cut(s) 75, 220, 380, 1219, 1649, 1694, 1747, 2264
CviAII CATG 9 cut(s) 223, 467, 635, 725, 1136, 1228, 2368, 2608, 2669
CviQI GTAC 8 cut(s) 75, 220, 380, 1219, 1649, 1694, 1747, 2264
DdeI CTNAG 9 cut(s) 264, 429, 755, 1678, 1757, 2047, 2066, 2331, 2628
DraI TTTAAA 1 cut(s) 2244
DrdI GACNNNNNNGTC 1 cut(s) 176
DseDI GACNNNNNNGTC 1 cut(s) 176
EaeI YGGCCR 1 cut(s) 2398
Eam1104I CTCTTC 2 cut(s) 483, 2046
EarI CTCTTC 2 cut(s) 483, 2046
Ecl136II GAGCTC 1 cut(s) 2374
Eco105I TACGTA 2 cut(s) 918, 2489
Eco130I CCWWGG 1 cut(s) 607
Eco147I AGGCCT 1 cut(s) 1059
Eco24I GRGCYC 3 cut(s) 109, 702, 2376
Eco31I GGTCTC 1 cut(s) 265
Eco32I GATATC 3 cut(s) 733, 1393, 1552
Eco47I GGWCC 5 cut(s) 178, 425, 557, 613, 2281
Eco53kI GAGCTC 1 cut(s) 2374
Eco57I CTGAAG 1 cut(s) 212
Eco81I CCTNAGG 1 cut(s) 429
Eco88I CYCGRG 1 cut(s) 1634
EcoICRI GAGCTC 1 cut(s) 2374
EcoO109I RGGNCCY 3 cut(s) 105, 425, 557
EcoRI GAATTC 3 cut(s) 1510, 1987, 2618
EcoRII CCWGG 8 cut(s) 174, 559, 792, 908, 1064, 1836, 2400, 2698
EcoRV GATATC 3 cut(s) 733, 1393, 1552
EcoT14I CCWWGG 1 cut(s) 607
EcoT22I ATGCAT 1 cut(s) 1137
EcoT38I GRGCYC 3 cut(s) 109, 702, 2376
ErhI CCWWGG 1 cut(s) 607
Esp3I CGTCTC 1 cut(s) 32
FaeI CATG 9 cut(s) 226, 470, 638, 728, 1139, 1231, 2371, 2611, 2672
FalI AAGNNNNNCTT 4 cut(s) 987, 1019, 2580, 2612
FaqI GGGAC 2 cut(s) 182, 451
FatI CATG 9 cut(s) 222, 466, 634, 724, 1135, 1227, 2367, 2607, 2668
FauNDI CATATG 1 cut(s) 2199
FbaI TGATCA 1 cut(s) 742
FblI GTMKAC 2 cut(s) 914, 920
Fnu4HI GCNGC 5 cut(s) 21, 406, 619, 1935, 2252
FokI GGATG 8 cut(s) 152, 218, 240, 278, 2168, 2189, 2339, 2682
FriOI GRGCYC 3 cut(s) 109, 702, 2376
Fsp4HI GCNGC 5 cut(s) 21, 406, 619, 1935, 2252
FspBI CTAG 7 cut(s) 165, 371, 879, 1094, 1167, 1607, 1959
FspI TGCGCA 1 cut(s) 1938
GlaI GCGC 4 cut(s) 273, 1883, 1938, 2346
GluI GCNGC 5 cut(s) 21, 406, 619, 1935, 2252
GsuI CTGGAG 6 cut(s) 310, 400, 582, 892, 1040, 2512
HaeII RGCGCY 1 cut(s) 2348
HaeIII GGCC 6 cut(s) 107, 405, 1059, 1300, 1633, 2400
HapII CCGG 9 cut(s) 109, 257, 570, 852, 899, 1615, 1715, 2208, 2279
HgaI GACGC 1 cut(s) 1539
HhaI GCGC 4 cut(s) 274, 1884, 1939, 2347
Hin1I GRCGYC 1 cut(s) 1531
Hin1II CATG 9 cut(s) 226, 470, 638, 728, 1139, 1231, 2371, 2611, 2672
Hin6I GCGC 4 cut(s) 272, 1882, 1937, 2345
HinP1I GCGC 4 cut(s) 272, 1882, 1937, 2345
HincII GTYRAC 3 cut(s) 664, 1912, 2386
HindII GTYRAC 3 cut(s) 664, 1912, 2386
HindIII AAGCTT 2 cut(s) 816, 1799
HinfI GANTC 8 cut(s) 374, 1404, 1579, 1718, 1753, 2140, 2275, 2721
HpaII CCGG 9 cut(s) 109, 257, 570, 852, 899, 1615, 1715, 2208, 2279
HphI GGTGA 3 cut(s) 936, 1852, 2582
Hpy166II GTNNAC 9 cut(s) 598, 664, 863, 915, 921, 1694, 1708, 1912, 2386
Hpy188I TCNGA 9 cut(s) 388, 872, 1153, 1509, 1521, 2130, 2417, 2538, 2716
Hpy8I GTNNAC 9 cut(s) 598, 664, 863, 915, 921, 1694, 1708, 1912, 2386
Hpy99I CGWCG 2 cut(s) 1533, 2655
HpyAV CCTTC 9 cut(s) 395, 970, 989, 1049, 1070, 1280, 2064, 2234, 2488
HpyCH4III ACNGT 6 cut(s) 71, 135, 454, 1321, 1474, 2271
HpyCH4IV ACGT 5 cut(s) 365, 917, 1749, 1925, 2488
HpyCH4V TGCA 8 cut(s) 130, 143, 839, 1135, 1457, 1687, 1708, 2203
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 411, 706, 2515
HpyF3I CTNAG 9 cut(s) 264, 429, 755, 1678, 1757, 2047, 2066, 2331, 2628
HpySE526I ACGT 5 cut(s) 365, 917, 1749, 1925, 2488
Hsp92I GRCGYC 1 cut(s) 1531
Hsp92II CATG 9 cut(s) 226, 470, 638, 728, 1139, 1231, 2371, 2611, 2672
HspAI GCGC 4 cut(s) 272, 1882, 1937, 2345
Kpn2I TCCGGA 2 cut(s) 1714, 2278
KpnI GGTACC 1 cut(s) 2267
Ksp22I TGATCA 1 cut(s) 742
LmnI GCTCC 6 cut(s) 419, 572, 807, 901, 1931, 2371
Lsp1109I GCAGC 2 cut(s) 630, 1921
LweI GCATC 5 cut(s) 130, 196, 1122, 2317, 2704
MabI ACCWGGT 1 cut(s) 174
MaeI CTAG 7 cut(s) 165, 371, 879, 1094, 1167, 1607, 1959
MaeII ACGT 5 cut(s) 365, 917, 1749, 1925, 2488
MaeIII GTNAC 7 cut(s) 314, 366, 1178, 1840, 1992, 2117, 2547
MboII GAAGA 9 cut(s) 473, 500, 740, 961, 1138, 1141, 1250, 2063, 2600
MfeI CAATTG 1 cut(s) 543
MflI RGATCY 7 cut(s) 94, 1148, 1186, 2062, 2086, 2210, 2728
MhlI GDGCHC 4 cut(s) 109, 702, 1710, 2376
MlsI TGGCCA 1 cut(s) 2400
MluNI TGGCCA 1 cut(s) 2400
MlyI GAGTC 3 cut(s) 1413, 1588, 1762
MmeI TCCRAC 5 cut(s) 532, 1449, 1791, 2254, 2653
Mox20I TGGCCA 1 cut(s) 2400
Mph1103I ATGCAT 1 cut(s) 1137
MroI TCCGGA 2 cut(s) 1714, 2278
MroXI GAANNNNTTC 3 cut(s) 717, 1059, 2595
MscI TGGCCA 1 cut(s) 2400
MslI CAYNNNNRTG 3 cut(s) 387, 1838, 2743
Msp20I TGGCCA 1 cut(s) 2400
MspI CCGG 9 cut(s) 109, 257, 570, 852, 899, 1615, 1715, 2208, 2279
MunI CAATTG 1 cut(s) 543
MvaI CCWGG 8 cut(s) 176, 561, 794, 910, 1066, 1838, 2402, 2700
MvnI CGCG 1 cut(s) 1437
MwoI GCNNNNNNNGC 4 cut(s) 17, 411, 706, 2515
NciI CCSGG 3 cut(s) 109, 853, 2208
NdeI CATATG 1 cut(s) 2199
NheI GCTAGC 1 cut(s) 1093
NlaIII CATG 9 cut(s) 226, 470, 638, 728, 1139, 1231, 2371, 2611, 2672
NlaIV GGNNCC 8 cut(s) 107, 234, 394, 568, 614, 1150, 2212, 2265
NmeAIII GCCGAG 2 cut(s) 1280, 2671
NmuCI GTSAC 1 cut(s) 1840
NruI TCGCGA 1 cut(s) 1437
NsbI TGCGCA 1 cut(s) 1938
NsiI ATGCAT 1 cut(s) 1137
NspI RCATGY 4 cut(s) 226, 1231, 2611, 2672
NspV TTCGAA 1 cut(s) 1985
OliI CACNNNNGTG 1 cut(s) 1838
PagI TCATGA 1 cut(s) 724
PasI CCCWGGG 1 cut(s) 1065
PceI AGGCCT 1 cut(s) 1059
PdmI GAANNNNTTC 3 cut(s) 717, 1059, 2595
PfeI GAWTC 5 cut(s) 374, 1718, 2140, 2275, 2721
Pfl23II CGTACG 1 cut(s) 74
PflMI CCANNNNNTGG 4 cut(s) 187, 472, 514, 1891
PfoI TCCNGGA 3 cut(s) 559, 792, 2698
PkrI GCNGC 5 cut(s) 22, 407, 620, 1936, 2253
PleI GAGTC 3 cut(s) 1412, 1587, 1761
PpsI GAGTC 3 cut(s) 1412, 1587, 1761
Ppu21I YACGTR 3 cut(s) 918, 1750, 2489
PpuMI RGGWCCY 2 cut(s) 425, 557
PsiI TTATAA 1 cut(s) 195
Psp124BI GAGCTC 1 cut(s) 2376
Psp5II RGGWCCY 2 cut(s) 425, 557
Psp6I CCWGG 8 cut(s) 174, 559, 792, 908, 1064, 1836, 2400, 2698
PspGI CCWGG 8 cut(s) 174, 559, 792, 908, 1064, 1836, 2400, 2698
PspLI CGTACG 1 cut(s) 74
PspN4I GGNNCC 8 cut(s) 107, 234, 394, 568, 614, 1150, 2212, 2265
PspOMI GGGCCC 1 cut(s) 105
PspPI GGNCC 8 cut(s) 105, 106, 178, 425, 557, 613, 1632, 2281
PspPPI RGGWCCY 2 cut(s) 425, 557
PsuI RGATCY 7 cut(s) 94, 1148, 1186, 2062, 2086, 2210, 2728
RruI TCGCGA 1 cut(s) 1437
RsaI GTAC 8 cut(s) 76, 221, 381, 1220, 1650, 1695, 1748, 2265
RsaNI GTAC 8 cut(s) 75, 220, 380, 1219, 1649, 1694, 1747, 2264
RseI CAYNNNNRTG 3 cut(s) 387, 1838, 2743
SacI GAGCTC 1 cut(s) 2376
SatI GCNGC 5 cut(s) 21, 406, 619, 1935, 2252
Sau96I GGNCC 8 cut(s) 105, 106, 178, 425, 557, 613, 1632, 2281
SchI GAGTC 3 cut(s) 1413, 1588, 1762
SduI GDGCHC 4 cut(s) 109, 702, 1710, 2376
SexAI ACCWGGT 1 cut(s) 174
SfaNI GCATC 5 cut(s) 130, 196, 1122, 2317, 2704
SfuI TTCGAA 1 cut(s) 1985
SinI GGWCC 5 cut(s) 178, 425, 557, 613, 2281
SmiMI CAYNNNNRTG 3 cut(s) 387, 1838, 2743
SmlI CTYRAG 2 cut(s) 2339, 2510
SmoI CTYRAG 2 cut(s) 2339, 2510
SnaBI TACGTA 2 cut(s) 918, 2489
SpeI ACTAGT 1 cut(s) 1606
SseBI AGGCCT 1 cut(s) 1059
SsiI CCGC 6 cut(s) 21, 406, 616, 1328, 1334, 2251
SspI AATATT 3 cut(s) 199, 886, 1501
SspMI CTAG 7 cut(s) 165, 371, 879, 1094, 1167, 1607, 1959
SstI GAGCTC 1 cut(s) 2376
StuI AGGCCT 1 cut(s) 1059
StyI CCWWGG 1 cut(s) 607
TaaI ACNGT 6 cut(s) 71, 135, 454, 1321, 1474, 2271
TaiI ACGT 5 cut(s) 368, 920, 1752, 1928, 2491
TaqI TCGA 4 cut(s) 1395, 1528, 1985, 2376
TatI WGTACW 3 cut(s) 219, 379, 1218
TauI GCSGC 3 cut(s) 23, 408, 2254
TfiI GAWTC 5 cut(s) 374, 1718, 2140, 2275, 2721
TscAI CASTG 8 cut(s) 140, 742, 1387, 1477, 1550, 2276, 2401, 2562
TseFI GTSAC 1 cut(s) 1840
TseI GCWGC 2 cut(s) 618, 1934
Tsp45I GTSAC 1 cut(s) 1840
TspGWI ACGGA 2 cut(s) 77, 1150
TspRI CASTG 8 cut(s) 140, 742, 1387, 1477, 1550, 2276, 2401, 2562
Van91I CCANNNNNTGG 4 cut(s) 187, 472, 514, 1891
VneI GTGCAC 1 cut(s) 1706
VpaK11BI GGWCC 5 cut(s) 178, 425, 557, 613, 2281
XceI RCATGY 4 cut(s) 226, 1231, 2611, 2672
XcmI CCANNNNNNNNNTGG 2 cut(s) 932, 2131
XmiI GTMKAC 2 cut(s) 914, 920
XmnI GAANNNNTTC 3 cut(s) 717, 1059, 2595
XspI CTAG 7 cut(s) 165, 371, 879, 1094, 1167, 1607, 1959
Zsp2I ATGCAT 1 cut(s) 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.