RLG00000033046

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000865
Physical Location & Seq
Forward (+)
22558135 .. 22559907
1773 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033046

Sequence Viewer

Length: 723 bp
ATGCCAGAGCTTTCAACACTTGATGTACAATCAACAAGCGAAGATGAGTATCTTGATTTGGATCAACTGAATTCCCCTCCAGGGCCTCCCCGCTATCTCCAACGTCTATCTTTGACAGGGCGCCTAGAAAGTCTACCACAGTGGATTCCCCAGCTTCACAGTCTAACTAGGATTGCTTTGAAGTGGTCTAAACTGAATGCTGATGTCAACTCACTTGAGGCGCTCCAAGATTTGCCTAATCTGTTGGAGCTACAGTTGGTGGACTATTTGACTGGGGATTCATTGAAATTCAAAGCCAAAAAGTTCAAGAAACTAAAGGTATTACGTATTGAGCAACTTGATCAGCTAGATATGGTGGTAGTCGAGGATGGAGCAATGCCCGAGCTTAAGAAGTTAACATTGTCTAGATGTCAGAATCTGAAGTTACTTCCATTTGGCATTGATAGGCTTATCTTGCTTGAAGAATTGATTCTGTATGACATGCCCGAAGAATTCATTGCTAGGCTTCAGAAGGATAGCGAAGACCGTTACTTGGTTGAGAATCTTCGATCCCAACAAAACAGATCATATTTCACTCGAAAGTATGATCCAAACCCCATTGAAGATGACCATCTACAATTTGGGTACAATATAACGCAACATGCTGTCTTAATCGGTGAGACATGGTGCCTCCACTTTTTGCAGATGCGGTGTCACAGGCTTTTGCAGATACACATGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

241

Amino Acids

28.14

Weight (kDa)

5.43

Isoelectric Point (pI)

56.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 3 - 157 9.8e-14 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 120, 666
AccI GTMKAC 1 cut(s) 133
AciI CCGC 2 cut(s) 91, 688
AclWI GGATC 3 cut(s) 69, 543, 581
AcsI RAATTY 3 cut(s) 70, 287, 491
AcuI CTGAAG 2 cut(s) 440, 491
AcyI GRCGYC 1 cut(s) 121
AfaI GTAC 2 cut(s) 27, 626
AfiI CCNNNNNNNGG 2 cut(s) 81, 532
AflII CTTAAG 1 cut(s) 386
AgsI TTSAA 7 cut(s) 15, 181, 286, 292, 307, 461, 602
AjnI CCWGG 1 cut(s) 79
AluBI AGCT 5 cut(s) 10, 154, 250, 346, 385
AluI AGCT 5 cut(s) 10, 154, 250, 346, 385
Alw26I GTCTC 1 cut(s) 653
AlwI GGATC 3 cut(s) 69, 543, 581
AlwNI CAGNNNCTG 1 cut(s) 418
Ama87I CYCGRG 1 cut(s) 380
AoxI GGCC 1 cut(s) 83
ApoI RAATTY 3 cut(s) 70, 287, 491
Asp700I GAANNNNTTC 1 cut(s) 468
AspLEI GCGC 2 cut(s) 123, 223
AspS9I GGNCC 1 cut(s) 83
AsuHPI GGTGA 1 cut(s) 668
AvaI CYCGRG 1 cut(s) 380
BanI GGYRCC 2 cut(s) 120, 666
BbsI GAAGAC 1 cut(s) 528
BccI CCATC 2 cut(s) 362, 618
BciT130I CCWGG 1 cut(s) 81
BclI TGATCA 1 cut(s) 340
BcoDI GTCTC 1 cut(s) 653
BfaI CTAG 6 cut(s) 125, 168, 347, 405, 501, 721
BfmI CTRYAG 1 cut(s) 251
BfoI RGCGCY 2 cut(s) 124, 224
BfrI CTTAAG 1 cut(s) 386
Bme1390I CCNGG 1 cut(s) 81
BmeT110I CYCGRG 1 cut(s) 380
BmgT120I GGNCC 1 cut(s) 83
BmiI GGNNCC 2 cut(s) 122, 668
BmrFI CCNGG 1 cut(s) 81
BmrI ACTGGG 1 cut(s) 282
BmsI GCATC 1 cut(s) 675
BmuI ACTGGG 1 cut(s) 282
BpiI GAAGAC 1 cut(s) 528
BpmI CTGGAG 1 cut(s) 63
BpuEI CTTGAG 1 cut(s) 236
BsaAI YACGTR 1 cut(s) 326
BsaBI GATNNNNATC 3 cut(s) 48, 60, 609
BsaHI GRCGYC 1 cut(s) 121
BsaJI CCNNGG 1 cut(s) 80
BsaXI ACNNNNNCTCC 2 cut(s) 239, 269
Bsc4I CCNNNNNNNGG 2 cut(s) 81, 532
Bse1I ACTGG 1 cut(s) 277
Bse3DI GCAATG 2 cut(s) 381, 495
Bse8I GATNNNNATC 3 cut(s) 48, 60, 609
BseBI CCWGG 1 cut(s) 81
BseDI CCNNGG 1 cut(s) 80
BseGI GGATG 1 cut(s) 373
BseJI GATNNNNATC 3 cut(s) 48, 60, 609
BseLI CCNNNNNNNGG 2 cut(s) 81, 532
BseMI GCAATG 2 cut(s) 381, 495
BseNI ACTGG 1 cut(s) 277
BseYI CCCAGC 1 cut(s) 150
BshFI GGCC 1 cut(s) 85
BshNI GGYRCC 2 cut(s) 120, 666
BsiHKCI CYCGRG 1 cut(s) 380
BslI CCNNNNNNNGG 2 cut(s) 81, 532
BsmAI GTCTC 1 cut(s) 653
BsmI GAATGC 1 cut(s) 202
BsnI GGCC 1 cut(s) 85
BsoBI CYCGRG 1 cut(s) 380
Bsp1407I TGTACA 1 cut(s) 25
Bsp143I GATC 5 cut(s) 61, 340, 548, 563, 586
BspACI CCGC 2 cut(s) 91, 688
BspANI GGCC 1 cut(s) 85
BspLI GGNNCC 2 cut(s) 122, 668
BspPI GGATC 3 cut(s) 69, 543, 581
BspT107I GGYRCC 2 cut(s) 120, 666
BspTI CTTAAG 1 cut(s) 386
BsrDI GCAATG 2 cut(s) 381, 495
BsrGI TGTACA 1 cut(s) 25
BsrI ACTGG 1 cut(s) 277
BssECI CCNNGG 1 cut(s) 80
BssMI GATC 5 cut(s) 61, 340, 548, 563, 586
BssNI GRCGYC 1 cut(s) 121
Bst2UI CCWGG 1 cut(s) 81
Bst4CI ACNGT 4 cut(s) 141, 161, 255, 527
BstACI GRCGYC 1 cut(s) 121
BstAFI CTTAAG 1 cut(s) 386
BstAUI TGTACA 1 cut(s) 25
BstBAI YACGTR 1 cut(s) 326
BstF5I GGATG 1 cut(s) 373
BstH2I RGCGCY 2 cut(s) 124, 224
BstHHI GCGC 2 cut(s) 123, 223
BstKTI GATC 5 cut(s) 64, 343, 551, 566, 589
BstMAI GTCTC 1 cut(s) 653
BstMBI GATC 5 cut(s) 61, 340, 548, 563, 586
BstMWI GCNNNNNNNGC 1 cut(s) 454
BstNI CCWGG 1 cut(s) 81
BstNSI RCATGY 3 cut(s) 484, 644, 718
BstSCI CCNGG 1 cut(s) 79
BstSFI CTRYAG 1 cut(s) 251
BstSNI TACGTA 1 cut(s) 326
BstV2I GAAGAC 1 cut(s) 528
BsuRI GGCC 1 cut(s) 85
BtsCI GGATG 1 cut(s) 373
BtsIMutI CAGTG 1 cut(s) 146
CaiI CAGNNNCTG 1 cut(s) 418
CfoI GCGC 2 cut(s) 123, 223
Cfr13I GGNCC 1 cut(s) 83
Csp6I GTAC 2 cut(s) 26, 625
CviAII CATG 4 cut(s) 481, 641, 663, 715
CviQI GTAC 2 cut(s) 26, 625
DinI GGCGCC 1 cut(s) 122
DpnI GATC 5 cut(s) 63, 342, 550, 565, 588
DpnII GATC 5 cut(s) 61, 340, 548, 563, 586
Eco105I TACGTA 1 cut(s) 326
Eco57I CTGAAG 2 cut(s) 440, 491
Eco88I CYCGRG 1 cut(s) 380
EcoO109I RGGNCCY 1 cut(s) 83
EcoRI GAATTC 2 cut(s) 70, 491
EcoRII CCWGG 1 cut(s) 79
EgeI GGCGCC 1 cut(s) 122
EheI GGCGCC 1 cut(s) 122
FaeI CATG 4 cut(s) 484, 644, 666, 718
FaiI YATR 9 cut(s) 353, 477, 482, 568, 585, 632, 642, 664, 716
FatI CATG 4 cut(s) 480, 640, 662, 714
FauI CCCGC 1 cut(s) 98
FbaI TGATCA 1 cut(s) 340
FblI GTMKAC 1 cut(s) 133
FokI GGATG 1 cut(s) 380
FspBI CTAG 6 cut(s) 125, 168, 347, 405, 501, 721
GlaI GCGC 2 cut(s) 122, 222
GsaI CCCAGC 1 cut(s) 154
GsuI CTGGAG 1 cut(s) 63
HaeII RGCGCY 2 cut(s) 124, 224
HaeIII GGCC 1 cut(s) 85
HhaI GCGC 2 cut(s) 123, 223
Hin1I GRCGYC 1 cut(s) 121
Hin1II CATG 4 cut(s) 484, 644, 666, 718
Hin6I GCGC 2 cut(s) 121, 221
HinP1I GCGC 2 cut(s) 121, 221
HincII GTYRAC 2 cut(s) 208, 396
HindII GTYRAC 2 cut(s) 208, 396
HinfI GANTC 5 cut(s) 145, 278, 415, 469, 541
HpaI GTTAAC 1 cut(s) 396
HphI GGTGA 1 cut(s) 668
Hpy166II GTNNAC 4 cut(s) 134, 208, 262, 396
Hpy188I TCNGA 3 cut(s) 414, 420, 510
Hpy188III TCNNGA 3 cut(s) 53, 307, 405
Hpy8I GTNNAC 4 cut(s) 134, 208, 262, 396
HpyAV CCTTC 1 cut(s) 505
HpyCH4III ACNGT 4 cut(s) 141, 161, 255, 527
HpyCH4IV ACGT 2 cut(s) 103, 325
HpyCH4V TGCA 2 cut(s) 682, 706
HpyF10VI GCNNNNNNNGC 1 cut(s) 454
HpySE526I ACGT 2 cut(s) 103, 325
Hsp92I GRCGYC 1 cut(s) 121
Hsp92II CATG 4 cut(s) 484, 644, 666, 718
HspAI GCGC 2 cut(s) 121, 221
KasI GGCGCC 1 cut(s) 120
Ksp22I TGATCA 1 cut(s) 340
KspAI GTTAAC 1 cut(s) 396
Kzo9I GATC 5 cut(s) 61, 340, 548, 563, 586
LmnI GCTCC 3 cut(s) 228, 247, 371
LpnPI CCDG 7 cut(s) 18, 66, 93, 102, 164, 258, 682
LweI GCATC 1 cut(s) 675
MaeI CTAG 6 cut(s) 125, 168, 347, 405, 501, 721
MaeII ACGT 2 cut(s) 103, 325
MaeIII GTNAC 3 cut(s) 423, 527, 692
MalI GATC 5 cut(s) 63, 342, 550, 565, 588
MboI GATC 5 cut(s) 61, 340, 548, 563, 586
MboII GAAGA 6 cut(s) 53, 473, 500, 533, 536, 614
MluCI AATT 5 cut(s) 70, 287, 464, 491, 617
Mly113I GGCGCC 1 cut(s) 121
MmeI TCCRAC 2 cut(s) 124, 225
MnlI CCTC 5 cut(s) 87, 96, 211, 358, 680
MroXI GAANNNNTTC 1 cut(s) 468
MseI TTAA 3 cut(s) 387, 395, 650
MspCI CTTAAG 1 cut(s) 386
MspR9I CCNGG 1 cut(s) 81
Mva1269I GAATGC 1 cut(s) 202
MvaI CCWGG 1 cut(s) 81
MwoI GCNNNNNNNGC 1 cut(s) 454
NarI GGCGCC 1 cut(s) 121
NdeII GATC 5 cut(s) 61, 340, 548, 563, 586
NlaIII CATG 4 cut(s) 484, 644, 666, 718
NlaIV GGNNCC 2 cut(s) 122, 668
NmuCI GTSAC 1 cut(s) 692
NspI RCATGY 3 cut(s) 484, 644, 718
PctI GAATGC 1 cut(s) 202
PdmI GAANNNNTTC 1 cut(s) 468
PfeI GAWTC 5 cut(s) 145, 278, 415, 469, 541
PluTI GGCGCC 1 cut(s) 124
Ppu21I YACGTR 1 cut(s) 326
Psp6I CCWGG 1 cut(s) 79
PspFI CCCAGC 1 cut(s) 150
PspGI CCWGG 1 cut(s) 79
PspN4I GGNNCC 2 cut(s) 122, 668
PspPI GGNCC 1 cut(s) 83
PstNI CAGNNNCTG 1 cut(s) 418
RsaI GTAC 2 cut(s) 27, 626
RsaNI GTAC 2 cut(s) 26, 625
SaqAI TTAA 3 cut(s) 387, 395, 650
Sau3AI GATC 5 cut(s) 61, 340, 548, 563, 586
Sau96I GGNCC 1 cut(s) 83
ScrFI CCNGG 1 cut(s) 81
SetI ASST 8 cut(s) 12, 106, 156, 252, 321, 328, 348, 387
SfaNI GCATC 1 cut(s) 675
SfcI CTRYAG 1 cut(s) 251
SfoI GGCGCC 1 cut(s) 122
SmlI CTYRAG 2 cut(s) 215, 386
SmoI CTYRAG 2 cut(s) 215, 386
SnaBI TACGTA 1 cut(s) 326
Sse9I AATT 5 cut(s) 70, 287, 464, 491, 617
SsiI CCGC 2 cut(s) 91, 688
SspDI GGCGCC 1 cut(s) 120
SspMI CTAG 6 cut(s) 125, 168, 347, 405, 501, 721
StyD4I CCNGG 1 cut(s) 79
TaaI ACNGT 4 cut(s) 141, 161, 255, 527
TaiI ACGT 2 cut(s) 106, 328
TaqI TCGA 3 cut(s) 363, 547, 577
TasI AATT 5 cut(s) 70, 287, 464, 491, 617
TatI WGTACW 1 cut(s) 25
TfiI GAWTC 5 cut(s) 145, 278, 415, 469, 541
Tru1I TTAA 3 cut(s) 387, 395, 650
Tru9I TTAA 3 cut(s) 387, 395, 650
TscAI CASTG 1 cut(s) 146
TseFI GTSAC 1 cut(s) 692
Tsp45I GTSAC 1 cut(s) 692
TspDTI ATGAA 2 cut(s) 270, 484
TspRI CASTG 1 cut(s) 146
Vha464I CTTAAG 1 cut(s) 386
XapI RAATTY 3 cut(s) 70, 287, 491
XbaI TCTAGA 1 cut(s) 404
XceI RCATGY 3 cut(s) 484, 644, 718
XcmI CCANNNNNNNNNTGG 1 cut(s) 617
XmiI GTMKAC 1 cut(s) 133
XmnI GAANNNNTTC 1 cut(s) 468
XspI CTAG 6 cut(s) 125, 168, 347, 405, 501, 721
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.