Rh4CG100000

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000865
Physical Location & Seq
Reverse (-)
18416927 .. 18420652
3726 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG100000.1

Sequence Viewer

Length: 2751 bp
ATGGCTGATCTAGCCGTTTCTGTAGCAATATTATTAGTTGAGAAGGTGATAAGCTCTTTGGACCAAAAGATCACTAGTAATGTCCATGGTGATGTTGAAAAGGCCTGGAAATGTTTGAGGAGGATGCAAGCCTATCTAAGGGACTCGGAAGAGATTGACGTAGGTAGTCAAATCTTTCAAAATCGTGTCAAAGAGGTCCAAAGTTTAGCTTATGAAATTGAGGATGCTATTGATAAGTTTATGGTTCAGATTCCACATCACTTCCACAGCAATATGTTCTCTGAGGCGATTCACGACATTGCCTACTTTTTCACAGAAAGAAGAGCAATAAGTGAGTTCTCCTCAAAAATTAAAGATATTGAAACGAGGGTTGGTAGCTTTTCAGCCTTGGATTCACTACTTCTTGAGCCAACAAGTTCTAGCGCAAGAACAGAAGAAAACCTGACCCACCAAGTTCTCGAGGAAGATGAGGTAGTGGGCTTTGAAGAGCGTAAAGGAACACTCCTCAAACAGTTGACAGAAGCAAATTCAAAGCTCTTGACGATTTTGATTGTAGGTCCAGGCGGCTCCGGCAAAACCACAATCGTGAAGAATGTTTATGACAGTAAACTGGTACAAGGATTTTTTGATTGCCATTCTTGGATTGATGTATCACGTCCTCCTAAACTTGGAAAGCTCTTGCGAAATATGTTAATCGATTTCAGACTGAAAAAGAATAACAGCGCTTGGAGTAAATATGAAGCTGTTAGACAGATTGTTCCAAGAGCGGGATTGAGACTATTTCCGCAGCAAAAACTGAAAAGACAAAAACCAGTTCATGATGATGAAGAAGAAGAAGAAGAAGAAGACGAAGAAGAAGAAGAAAAGGAAGAAGAAGAAGCAGCTGAAAATCCAAAAGCAGAATTGAATAATTTTCTGAAAGCTAAGAGGTTTCTGCTTGTGTTAGATAATGTTTGGAGAGTACAAGATCTGGAATCCATTGTAAATGCTCTACCAAAAGGATTGCCTGGGAGTAAAATAATCATAACTACACGGAAGTCTGATGTAGCTCCGGTTGCCACATATATCCATGATTTGAGTTGTGGTTTGCCATGGAAATATGCCTGGTATCTGTTCTGCAAGAAGGCTTTTCAAAGTAGTGAAGAAAATTGTCCACGCGAGCTCGAGCACTGGGCTAAGAAAATCCTGAAGAAGTGTGAAGGCTTGCCCCTTGCTATTTCAGCTGTGGGTACTTTGCTTTGGAAGAAGCCACAAACTCCAGTTGAGTGGAAGAAGTTACATGAAAGCCTTGGATCTGAAATTAGACCCGATTCTAGTCTTTCTGTTATTAGCCGCATTTTGCAGCCCAGTTATATGGATCTTCCAAGCCATCTGAAAACTTGTTTCTTATACTTTGGCATGTTTCCAGAAGACTATTTAATCAGTCGCGAGAAGCTGACTCGGTTGTGGTTAGCCGAAGGGTTTGTGATGCCAGGAAGAGGAAAAACAATGGAGGAGGTTGCAGAGAGTTATCTCAATGAACTCATTGCAAGAAACTTGGTTAATGCCACCAGAAGAGAGATAGATGGTCGAGTGAGGAGTTGTCGTGTTTTAAGCCTTGTCAGAGAGTTCATCATTACCAAGGCAGAAAACTTCATCACTGTTTTGGAGCCCAACTGCAGTACTACAACTTCAGGTGAGAAAATTCGTCGCCTTTCTGTTCAAAATGTCGAAATCAATTTGCTATTAAGAGATAGGGACTTGAGTCACATTCGTACCTTGCTAGTGTTTGGGCAGGCATGTTCCCCTTCTGATCTTGAAATGGTACTTAAAACCTCTAAATTTTTTAGGGTGTTTGATCTTCAAGGAGTACCTTTGAAGATTTTTCCCAAAAGTATTATTGGTCTCCGCCTTTTGAGGTACTTATGTCTGAGGCAAACTAAAATTAGATCAGTTCCACAGTCCATAAAAAATCTTTCATTCTTGCAAACTTTAGATCTGAGGTCCACCAAGGTGACAAAATTACCAAATACGATCTACAAATTCCAGAATATGCGTCATCTTTTAGCTTATAGCTGTGATATGAAATCTGGAGTGGCACGAGGAGTGGACGTGTCTGCGGGCAATATTGGATCGTTATCTTCCATACAGAAGTTGTTACTAGTCAAGGTGCAAAAAAACAGAAAAGCCATAAAAGCCTTGAGAGAGTTGATTGGTCTCCGGAAGTTGGGGCTGGTCGACCTTGGAAGAGAACATGGAAGAGAATTGTGTTGTTCCATTCAAAAGATGTCAGAGCTTTCATCACTTGTGGTACGATCAACAAGCGAGGAGGAGTATCTCGATTTGGATCATATGAATTCCCCCCCTGGTCCTCCTCTCTATCTCCAACGTCTATCTTTGACAGGGCGCTTAAAAATTCTACCGCAGTGGATAGCGCAGCTTCATAGTCTGACTAGGATTGCTTTGAAGGGTTCAAAACTGAATGCTGATGCCAACAGTACTGTTGAGGTCCTTCAAGATTTGCCTAATCTGATGGAACTAGAATTGGTTGACTCATTCACTGGGGTATCTTTGATATTCAAAGCTGAAAAGTTCAAGAAACTAAAGGCCTTGCGTATTGAGAAATTTGATCAGCTAGATTTGATGGTCGTCGAGGTTGGAGCAATGCCTCAGCTCAAGAAGTTAACCATGTCTAAATGCCAGAATCTGAAGTTACTTCCATTTGGCATTGGTCATCTAATCTTCCTCGAAGAACTGCATGCTCTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

916

Amino Acids

104.31

Weight (kDa)

8.74

Isoelectric Point (pI)

48.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 88 3.5e-12 Rx N-terminal domain
NB-ARC PF00931 162 - 242 7.2e-12 NB-ARC domain
NB-ARC PF00931 283 - 380 1.4e-15 NB-ARC domain
WHD_DRP PF23559 467 - 538 9.6e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 583 - 913 7.1e-42 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 767
AccI GTMKAC 1 cut(s) 2217
AccII CGCG 2 cut(s) 1158, 1428
AccIII TCCGGA 1 cut(s) 2199
AciI CCGC 7 cut(s) 564, 767, 785, 1333, 1888, 2099, 2402
AclWI GGATC 4 cut(s) 1300, 1365, 2119, 2334
AcsI RAATTY 7 cut(s) 526, 1683, 1820, 2021, 2335, 2394, 2603
AcuI CTGAAG 3 cut(s) 1208, 1656, 2708
AfeI AGCGCT 1 cut(s) 724
AfiI CCNNNNNNNGG 5 cut(s) 138, 668, 767, 1478, 2206
AflIII ACRYGT 1 cut(s) 2091
AhlI ACTAGT 2 cut(s) 74, 2140
AjiI CACGTC 2 cut(s) 656, 2092
AjnI CCWGG 6 cut(s) 104, 559, 1006, 1103, 1471, 2344
AjuI GAANNNNNNNTTGG 2 cut(s) 354, 386
AleI CACNNNNGTG 2 cut(s) 584, 1991
Alw21I GWGCWC 2 cut(s) 1164, 1170
Alw26I GTCTC 3 cut(s) 769, 1889, 2201
AlwI GGATC 4 cut(s) 1300, 1365, 2119, 2334
AlwNI CAGNNNCTG 1 cut(s) 2686
Ama87I CYCGRG 2 cut(s) 458, 1163
Aor13HI TCCGGA 1 cut(s) 2199
Aor51HI AGCGCT 1 cut(s) 724
AoxI GGCC 2 cut(s) 102, 2586
ApeKI GCWGC 4 cut(s) 787, 881, 1342, 2416
ApoI RAATTY 7 cut(s) 526, 1683, 1820, 2021, 2335, 2394, 2603
AspLEI GCGC 4 cut(s) 425, 725, 2388, 2416
AspS9I GGNCC 6 cut(s) 61, 196, 557, 1983, 2348, 2488
AsuHPI GGTGA 4 cut(s) 58, 101, 1688, 2005
AvaI CYCGRG 2 cut(s) 458, 1163
AvaII GGWCC 6 cut(s) 61, 196, 557, 1983, 2348, 2488
BaeI ACNNNNGTAYC 6 cut(s) 1738, 1771, 1891, 1924, 2529, 2562
BanII GRGCYC 2 cut(s) 1164, 1653
BarI GAAGNNNNNNTAC 2 cut(s) 1654, 1686
BauI CACGAG 1 cut(s) 2079
BbsI GAAGAC 2 cut(s) 852, 1416
Bbv12I GWGCWC 2 cut(s) 1164, 1170
BbvCI CCTCAGC 1 cut(s) 2649
BbvI GCAGC 4 cut(s) 799, 893, 1354, 2428
BccI CCATC 4 cut(s) 1377, 1559, 2506, 2617
BciT130I CCWGG 6 cut(s) 106, 561, 1008, 1105, 1473, 2346
BclI TGATCA 1 cut(s) 2608
BcoDI GTCTC 3 cut(s) 769, 1889, 2201
BcuI ACTAGT 2 cut(s) 74, 2140
BfaI CTAG 9 cut(s) 11, 75, 420, 1314, 1763, 2141, 2433, 2519, 2615
BfmI CTRYAG 2 cut(s) 21, 1657
BfoI RGCGCY 2 cut(s) 726, 2389
BglII AGATCT 2 cut(s) 967, 1975
BisI GCNGC 6 cut(s) 565, 788, 882, 1333, 1343, 2417
BlsI GCNGC 6 cut(s) 566, 789, 883, 1334, 1344, 2418
BmcAI AGTACT 2 cut(s) 1663, 2479
Bme1390I CCNGG 6 cut(s) 106, 561, 1008, 1105, 1473, 2346
Bme18I GGWCC 6 cut(s) 61, 196, 557, 1983, 2348, 2488
BmeT110I CYCGRG 2 cut(s) 458, 1163
BmgBI CACGTC 2 cut(s) 656, 2092
BmgT120I GGNCC 6 cut(s) 61, 196, 557, 1983, 2348, 2488
BmiI GGNNCC 2 cut(s) 568, 1650
BmrFI CCNGG 6 cut(s) 106, 561, 1008, 1105, 1473, 2346
BmrI ACTGGG 3 cut(s) 1180, 1341, 2550
BmsI GCATC 4 cut(s) 114, 214, 1458, 2458
BmuI ACTGGG 3 cut(s) 1180, 1341, 2550
BoxI GACNNNNGTC 1 cut(s) 1743
BpiI GAAGAC 2 cut(s) 852, 1416
BplI GAGNNNNNCTC 2 cut(s) 326, 358
BpmI CTGGAG 2 cut(s) 1242, 2091
Bpu10I CCTNAGC 1 cut(s) 2649
BpuEI CTTGAG 4 cut(s) 425, 1762, 2200, 2639
Bsa29I ATCGAT 1 cut(s) 696
BsaBI GATNNNNATC 2 cut(s) 2116, 2325
BsaI GGTCTC 2 cut(s) 1889, 2201
BsaJI CCNNGG 9 cut(s) 85, 387, 1007, 1091, 1288, 1620, 1989, 2221, 2344
BsaWI WCCGGW 2 cut(s) 1051, 2199
BsaXI ACNNNNNCTCC 4 cut(s) 1570, 1600, 2076, 2106
Bsc4I CCNNNNNNNGG 5 cut(s) 138, 668, 767, 1478, 2206
Bse1I ACTGG 6 cut(s) 615, 812, 1175, 1259, 1347, 2545
Bse3DI GCAATG 3 cut(s) 297, 1524, 2649
Bse8I GATNNNNATC 2 cut(s) 2116, 2325
BseAI TCCGGA 1 cut(s) 2199
BseBI CCWGG 6 cut(s) 106, 561, 1008, 1105, 1473, 2346
BseCI ATCGAT 1 cut(s) 696
BseDI CCNNGG 9 cut(s) 85, 387, 1007, 1091, 1288, 1620, 1989, 2221, 2344
BseGI GGATG 2 cut(s) 129, 229
BseJI GATNNNNATC 2 cut(s) 2116, 2325
BseLI CCNNNNNNNGG 5 cut(s) 138, 668, 767, 1478, 2206
BseMI GCAATG 3 cut(s) 297, 1524, 2649
BseMII CTCAG 4 cut(s) 273, 1901, 1970, 2663
BseNI ACTGG 6 cut(s) 615, 812, 1175, 1259, 1347, 2545
BseRI GAGGAG 9 cut(s) 133, 331, 494, 1508, 1591, 2097, 2321, 2324, 2343
BseXI GCAGC 4 cut(s) 799, 893, 1354, 2428
Bsh1236I CGCG 2 cut(s) 1158, 1428
BshFI GGCC 2 cut(s) 104, 2588
BshVI ATCGAT 1 cut(s) 696
BsiHKAI GWGCWC 2 cut(s) 1164, 1170
BsiHKCI CYCGRG 2 cut(s) 458, 1163
BsiSI CCGG 3 cut(s) 570, 1052, 2200
BslFI GGGAC 2 cut(s) 155, 1751
BslI CCNNNNNNNGG 5 cut(s) 138, 668, 767, 1478, 2206
BsmAI GTCTC 3 cut(s) 769, 1889, 2201
BsmFI GGGAC 2 cut(s) 155, 1751
BsmI GAATGC 1 cut(s) 2467
BsnI GGCC 2 cut(s) 104, 2588
Bso31I GGTCTC 2 cut(s) 1889, 2201
BsoBI CYCGRG 2 cut(s) 458, 1163
Bsp1286I GDGCHC 3 cut(s) 1164, 1170, 1653
Bsp13I TCCGGA 1 cut(s) 2199
Bsp19I CCATGG 2 cut(s) 85, 1091
Bsp68I TCGCGA 1 cut(s) 1428
BspACI CCGC 7 cut(s) 564, 767, 785, 1333, 1888, 2099, 2402
BspANI GGCC 2 cut(s) 104, 2588
BspCNI CTCAG 4 cut(s) 274, 1902, 1971, 2662
BspDI ATCGAT 1 cut(s) 696
BspEI TCCGGA 1 cut(s) 2199
BspFNI CGCG 2 cut(s) 1158, 1428
BspHI TCATGA 1 cut(s) 817
BspLI GGNNCC 2 cut(s) 568, 1650
BspMAI CTGCAG 1 cut(s) 1661
BspPI GGATC 4 cut(s) 1300, 1365, 2119, 2334
BspQI GCTCTTC 2 cut(s) 316, 480
BspTNI GGTCTC 2 cut(s) 1889, 2201
BsrBI CCGCTC 1 cut(s) 767
BsrDI GCAATG 3 cut(s) 297, 1524, 2649
BsrI ACTGG 6 cut(s) 615, 812, 1175, 1259, 1347, 2545
BssECI CCNNGG 9 cut(s) 85, 387, 1007, 1091, 1288, 1620, 1989, 2221, 2344
BssSI CACGAG 1 cut(s) 2079
BssT1I CCWWGG 7 cut(s) 85, 387, 1091, 1288, 1620, 1989, 2221
Bst2BI CACGAG 1 cut(s) 2079
Bst2UI CCWGG 6 cut(s) 106, 561, 1008, 1105, 1473, 2346
Bst4CI ACNGT 6 cut(s) 513, 605, 1642, 1941, 2477, 2482
Bst6I CTCTTC 7 cut(s) 144, 316, 480, 1471, 1549, 2221, 2233
BstC8I GCNNGC 6 cut(s) 129, 1160, 1205, 1776, 2101, 2739
BstDEI CTNAG 7 cut(s) 137, 282, 924, 1176, 1910, 1979, 2649
BstDSI CCRYGG 2 cut(s) 85, 1091
BstENI CCTNNNNNAGG 1 cut(s) 136
BstF5I GGATG 2 cut(s) 129, 229
BstFNI CGCG 2 cut(s) 1158, 1428
BstH2I RGCGCY 2 cut(s) 726, 2389
BstHHI GCGC 4 cut(s) 425, 725, 2388, 2416
BstMAI GTCTC 3 cut(s) 769, 1889, 2201
BstMWI GCNNNNNNNGC 5 cut(s) 11, 570, 1055, 1220, 2174
BstNI CCWGG 6 cut(s) 106, 561, 1008, 1105, 1473, 2346
BstNSI RCATGY 3 cut(s) 1402, 1782, 2741
BstPAI GACNNNNGTC 1 cut(s) 1743
BstSCI CCNGG 6 cut(s) 104, 559, 1006, 1103, 1471, 2344
BstSFI CTRYAG 2 cut(s) 21, 1657
BstUI CGCG 2 cut(s) 1158, 1428
BstV1I GCAGC 4 cut(s) 799, 893, 1354, 2428
BstV2I GAAGAC 2 cut(s) 852, 1416
BstX2I RGATCY 4 cut(s) 967, 1292, 1357, 1975
BstXI CCANNNNNNTGG 2 cut(s) 1266, 1354
BstYI RGATCY 4 cut(s) 967, 1292, 1357, 1975
Bsu15I ATCGAT 1 cut(s) 696
BsuRI GGCC 2 cut(s) 104, 2588
BsuTUI ATCGAT 1 cut(s) 696
BtgI CCRYGG 2 cut(s) 85, 1091
BtrI CACGTC 2 cut(s) 656, 2092
BtsCI GGATG 2 cut(s) 129, 229
BtsI GCAGTG 1 cut(s) 2411
BtsIMutI CAGTG 4 cut(s) 1168, 1638, 2411, 2538
BtuMI TCGCGA 1 cut(s) 1428
Cac8I GCNNGC 6 cut(s) 129, 1160, 1205, 1776, 2101, 2739
CaiI CAGNNNCTG 1 cut(s) 2686
CciI TCATGA 1 cut(s) 817
CfoI GCGC 4 cut(s) 425, 725, 2388, 2416
Cfr13I GGNCC 6 cut(s) 61, 196, 557, 1983, 2348, 2488
ClaI ATCGAT 1 cut(s) 696
CseI GACGC 1 cut(s) 2024
DdeI CTNAG 7 cut(s) 137, 282, 924, 1176, 1910, 1979, 2649
Eam1104I CTCTTC 7 cut(s) 144, 316, 480, 1471, 1549, 2221, 2233
EarI CTCTTC 7 cut(s) 144, 316, 480, 1471, 1549, 2221, 2233
EciI GGCGGA 1 cut(s) 1877
Ecl136II GAGCTC 1 cut(s) 1162
Eco130I CCWWGG 7 cut(s) 85, 387, 1091, 1288, 1620, 1989, 2221
Eco147I AGGCCT 2 cut(s) 104, 2588
Eco24I GRGCYC 2 cut(s) 1164, 1653
Eco31I GGTCTC 2 cut(s) 1889, 2201
Eco47I GGWCC 6 cut(s) 61, 196, 557, 1983, 2348, 2488
Eco47III AGCGCT 1 cut(s) 724
Eco53kI GAGCTC 1 cut(s) 1162
Eco57I CTGAAG 3 cut(s) 1208, 1656, 2708
Eco88I CYCGRG 2 cut(s) 458, 1163
EcoICRI GAGCTC 1 cut(s) 1162
EcoNI CCTNNNNNAGG 1 cut(s) 136
EcoO109I RGGNCCY 1 cut(s) 2488
EcoRI GAATTC 1 cut(s) 2335
EcoRII CCWGG 6 cut(s) 104, 559, 1006, 1103, 1471, 2344
EcoT14I CCWWGG 7 cut(s) 85, 387, 1091, 1288, 1620, 1989, 2221
EcoT38I GRGCYC 2 cut(s) 1164, 1653
ErhI CCWWGG 7 cut(s) 85, 387, 1091, 1288, 1620, 1989, 2221
FalI AAGNNNNNCTT 2 cut(s) 193, 225
FaqI GGGAC 2 cut(s) 155, 1751
FauI CCCGC 2 cut(s) 760, 2092
FauNDI CATATG 1 cut(s) 2331
FbaI TGATCA 1 cut(s) 2608
FblI GTMKAC 1 cut(s) 2217
Fnu4HI GCNGC 6 cut(s) 565, 788, 882, 1333, 1343, 2417
FokI GGATG 2 cut(s) 136, 236
FriOI GRGCYC 2 cut(s) 1164, 1653
Fsp4HI GCNGC 6 cut(s) 565, 788, 882, 1333, 1343, 2417
FspBI CTAG 9 cut(s) 11, 75, 420, 1314, 1763, 2141, 2433, 2519, 2615
GlaI GCGC 4 cut(s) 424, 724, 2387, 2415
GluI GCNGC 6 cut(s) 565, 788, 882, 1333, 1343, 2417
GsuI CTGGAG 2 cut(s) 1242, 2091
HaeII RGCGCY 2 cut(s) 726, 2389
HaeIII GGCC 2 cut(s) 104, 2588
HapII CCGG 3 cut(s) 570, 1052, 2200
HgaI GACGC 1 cut(s) 2024
HhaI GCGC 4 cut(s) 425, 725, 2388, 2416
Hin6I GCGC 4 cut(s) 423, 723, 2386, 2414
HinP1I GCGC 4 cut(s) 423, 723, 2386, 2414
HincII GTYRAC 4 cut(s) 516, 2218, 2530, 2664
HindII GTYRAC 4 cut(s) 516, 2218, 2530, 2664
HpaI GTTAAC 1 cut(s) 2664
HpaII CCGG 3 cut(s) 570, 1052, 2200
HphI GGTGA 4 cut(s) 58, 101, 1688, 2005
Hpy166II GTNNAC 8 cut(s) 516, 608, 1154, 1986, 2089, 2218, 2530, 2664
Hpy8I GTNNAC 8 cut(s) 516, 608, 1154, 1986, 2089, 2218, 2530, 2664
Hpy99I CGWCG 2 cut(s) 1692, 2633
HpyAV CCTTC 7 cut(s) 37, 1117, 1193, 1451, 1797, 2440, 2501
HpyCH4III ACNGT 6 cut(s) 513, 605, 1642, 1941, 2477, 2482
HpyCH4IV ACGT 4 cut(s) 159, 655, 2091, 2368
HpyCH4V TGCA 9 cut(s) 127, 1119, 1342, 1502, 1529, 1659, 1966, 2152, 2737
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 570, 1055, 1220, 2174
HpyF3I CTNAG 7 cut(s) 137, 282, 924, 1176, 1910, 1979, 2649
HpySE526I ACGT 4 cut(s) 159, 655, 2091, 2368
HspAI GCGC 4 cut(s) 423, 723, 2386, 2414
Kpn2I TCCGGA 1 cut(s) 2199
Ksp22I TGATCA 1 cut(s) 2608
KspAI GTTAAC 1 cut(s) 2664
LguI GCTCTTC 2 cut(s) 316, 480
LmnI GCTCC 4 cut(s) 572, 1054, 1648, 2639
Lsp1109I GCAGC 4 cut(s) 799, 893, 1354, 2428
LweI GCATC 4 cut(s) 114, 214, 1458, 2458
MaeI CTAG 9 cut(s) 11, 75, 420, 1314, 1763, 2141, 2433, 2519, 2615
MaeII ACGT 4 cut(s) 159, 655, 2091, 2368
MaeIII GTNAC 5 cut(s) 1275, 1745, 1993, 2136, 2691
MbiI CCGCTC 1 cut(s) 767
MflI RGATCY 4 cut(s) 967, 1292, 1357, 1975
MhlI GDGCHC 3 cut(s) 1164, 1170, 1653
MlyI GAGTC 4 cut(s) 137, 1432, 1753, 2525
MmeI TCCRAC 2 cut(s) 2389, 2617
MroI TCCGGA 1 cut(s) 2199
MseI TTAA 9 cut(s) 351, 692, 1418, 1542, 1592, 1727, 1809, 2390, 2663
MslI CAYNNNNRTG 4 cut(s) 90, 584, 822, 1991
MspA1I CMGCKG 2 cut(s) 884, 1223
MspI CCGG 3 cut(s) 570, 1052, 2200
MspR9I CCNGG 6 cut(s) 106, 561, 1008, 1105, 1473, 2346
Mva1269I GAATGC 1 cut(s) 2467
MvaI CCWGG 6 cut(s) 106, 561, 1008, 1105, 1473, 2346
MvnI CGCG 2 cut(s) 1158, 1428
MwoI GCNNNNNNNGC 5 cut(s) 11, 570, 1055, 1220, 2174
NcoI CCATGG 2 cut(s) 85, 1091
NdeI CATATG 1 cut(s) 2331
NlaIV GGNNCC 2 cut(s) 568, 1650
NmuCI GTSAC 2 cut(s) 1745, 1993
NruI TCGCGA 1 cut(s) 1428
NspI RCATGY 3 cut(s) 1402, 1782, 2741
OliI CACNNNNGTG 2 cut(s) 584, 1991
PaeI GCATGC 1 cut(s) 2741
PaeR7I CTCGAG 2 cut(s) 458, 1163
PagI TCATGA 1 cut(s) 817
PceI AGGCCT 2 cut(s) 104, 2588
PciSI GCTCTTC 2 cut(s) 316, 480
PctI GAATGC 1 cut(s) 2467
PfeI GAWTC 6 cut(s) 250, 289, 392, 974, 1310, 2683
PkrI GCNGC 6 cut(s) 566, 789, 883, 1334, 1344, 2418
PleI GAGTC 4 cut(s) 137, 1432, 1752, 2525
PpsI GAGTC 4 cut(s) 137, 1432, 1752, 2525
PpuMI RGGWCCY 1 cut(s) 2488
PshAI GACNNNNGTC 1 cut(s) 1743
Psp124BI GAGCTC 1 cut(s) 1164
Psp5II RGGWCCY 1 cut(s) 2488
Psp6I CCWGG 6 cut(s) 104, 559, 1006, 1103, 1471, 2344
PspGI CCWGG 6 cut(s) 104, 559, 1006, 1103, 1471, 2344
PspN4I GGNNCC 2 cut(s) 568, 1650
PspPI GGNCC 6 cut(s) 61, 196, 557, 1983, 2348, 2488
PspPPI RGGWCCY 1 cut(s) 2488
PspXI VCTCGAGB 1 cut(s) 1163
PstI CTGCAG 1 cut(s) 1661
PstNI CAGNNNCTG 1 cut(s) 2686
PsuI RGATCY 4 cut(s) 967, 1292, 1357, 1975
PvuII CAGCTG 2 cut(s) 884, 1223
RruI TCGCGA 1 cut(s) 1428
RseI CAYNNNNRTG 4 cut(s) 90, 584, 822, 1991
SacI GAGCTC 1 cut(s) 1164
SalI GTCGAC 1 cut(s) 2216
SapI GCTCTTC 2 cut(s) 316, 480
SaqAI TTAA 9 cut(s) 351, 692, 1418, 1542, 1592, 1727, 1809, 2390, 2663
SatI GCNGC 6 cut(s) 565, 788, 882, 1333, 1343, 2417
Sau96I GGNCC 6 cut(s) 61, 196, 557, 1983, 2348, 2488
ScaI AGTACT 2 cut(s) 1663, 2479
SchI GAGTC 4 cut(s) 137, 1432, 1753, 2525
ScrFI CCNGG 6 cut(s) 106, 561, 1008, 1105, 1473, 2346
SduI GDGCHC 3 cut(s) 1164, 1170, 1653
SfaNI GCATC 4 cut(s) 114, 214, 1458, 2458
SfcI CTRYAG 2 cut(s) 21, 1657
Sfr274I CTCGAG 2 cut(s) 458, 1163
SinI GGWCC 6 cut(s) 61, 196, 557, 1983, 2348, 2488
SlaI CTCGAG 2 cut(s) 458, 1163
SmiMI CAYNNNNRTG 4 cut(s) 90, 584, 822, 1991
SmlI CTYRAG 6 cut(s) 404, 458, 1163, 1741, 2179, 2654
SmoI CTYRAG 6 cut(s) 404, 458, 1163, 1741, 2179, 2654
SpeI ACTAGT 2 cut(s) 74, 2140
SphI GCATGC 1 cut(s) 2741
SseBI AGGCCT 2 cut(s) 104, 2588
SsiI CCGC 7 cut(s) 564, 767, 785, 1333, 1888, 2099, 2402
SspI AATATT 2 cut(s) 30, 2107
SspMI CTAG 9 cut(s) 11, 75, 420, 1314, 1763, 2141, 2433, 2519, 2615
SstI GAGCTC 1 cut(s) 1164
StuI AGGCCT 2 cut(s) 104, 2588
StyD4I CCNGG 6 cut(s) 104, 559, 1006, 1103, 1471, 2344
StyI CCWWGG 7 cut(s) 85, 387, 1091, 1288, 1620, 1989, 2221
TaaI ACNGT 6 cut(s) 513, 605, 1642, 1941, 2477, 2482
TaiI ACGT 4 cut(s) 162, 658, 2094, 2371
TaqI TCGA 9 cut(s) 459, 696, 1164, 1570, 1710, 2217, 2319, 2631, 2727
TatI WGTACW 3 cut(s) 961, 1661, 2477
TauI GCSGC 2 cut(s) 567, 1335
TfiI GAWTC 6 cut(s) 250, 289, 392, 974, 1310, 2683
Tru1I TTAA 9 cut(s) 351, 692, 1418, 1542, 1592, 1727, 1809, 2390, 2663
Tru9I TTAA 9 cut(s) 351, 692, 1418, 1542, 1592, 1727, 1809, 2390, 2663
TscAI CASTG 4 cut(s) 1175, 1645, 2411, 2545
TseFI GTSAC 2 cut(s) 1745, 1993
TseI GCWGC 4 cut(s) 787, 881, 1342, 2416
Tsp45I GTSAC 2 cut(s) 1745, 1993
TspGWI ACGGA 1 cut(s) 1048
TspRI CASTG 4 cut(s) 1175, 1645, 2411, 2545
VpaK11BI GGWCC 6 cut(s) 61, 196, 557, 1983, 2348, 2488
XagI CCTNNNNNAGG 1 cut(s) 136
XapI RAATTY 7 cut(s) 526, 1683, 1820, 2021, 2335, 2394, 2603
XceI RCATGY 3 cut(s) 1402, 1782, 2741
XhoI CTCGAG 2 cut(s) 458, 1163
XmiI GTMKAC 1 cut(s) 2217
XspI CTAG 9 cut(s) 11, 75, 420, 1314, 1763, 2141, 2433, 2519, 2615
ZrmI AGTACT 2 cut(s) 1663, 2479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.