Rw5G017850

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000865
Physical Location & Seq
Forward (+)
22820137 .. 22822945
2809 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G017850.1

Sequence Viewer

Length: 2652 bp
ATGGCTGAACTTGCAATTCCTTTATCATTGTTGGTGGCCGAGAAGGCGATGAGCTTGTGCGACCGAAGAAACAAGTCGGAGGACCCAAGTAATAATATCCATGATGATGTTGAAAGAGCCTGGAAATGTCTAAAGAGGATGCAAGCCTATCTAGGGGACTCGGAAGAGATTGATGAAGGCAGCGGCAGCCTAATGCTTCAAAATCGTGTCGCAGAAGTCCAAAGTCTGGCTTATGAAATAGAGGATGCTCTTGAGAAGTTCATATCTCAGATTCCACATCATTTCCATAGCAACTTATTCTCTCAGAAGCTGCACGACGTTACGCACTATCGCGAAGAAAAGAGAGCAAGAGTTGAGTTATCCACCAAGATTAAAGACATTGAAAAGAAAATTAGTTCCTTCTCAACTTTGGATTCACTTCTTGAGCCACCAAGTAGTTCCAGCACAAGGAAGAGGGATAGTTTGATCCACCAAGTTCTTGAGGAAGATGAAATGGTAGGCCTTGAGGAGCCTAAAGAAAGACTCTCCAAACAGTTGAGGGAAGAAGATTCAAGGCTCAAGACTATTTTGGTTGTAGGTCCTGGCGGCTCCGGCAAAACCACAGTTGTGAAGAATGTTTTTGACAGCACCATGGTCCAAATGTTTTTTGATTGCCATGCTTGGATCGACGTGTCACGGCCTCCTAAAGCAGAAGATCAGAAAGCTGAACTGATGCATCTGAAGGAAAAGAGATTTTTGGTTGTTTTGGATAATGTTTGGACAGCACAAGATCTGGAACTCATTACAAATGCTTTGCCAAGAGGATTGCCTGGGAGTAAAATAATCATAACTACTCGCAACTCTGATGTAGCTTCTTCTCGTGTTGAGTCCTCCGAGTATATCCATGATTTGAGTAGCGGGTTATCATGGAAAAAAGGCTGGTCTCTACTTTGCAAGAAGGCTTTTAGAAAAACTGCAGGATATTGTCCACCTGAACTTGAAGAGTGGGCTAAAAAGATCCTGAAAAGGTGTGAAGGTCTGCCCCTTGCAATTTCAGCTGTTGCTACTTTGCTGGCAAAGAAGCCACAAACTCCAGTTGAGTGGAAGAAGTTACATGAAAGCCTTGGGTCTGAAATTAGACCCGACTCTAGTCTTTCGATTATTAGCCGTGTCTTGCAGCCAAGTTACATGCATCTTCCGAGCCATCTGAAGATTTGTTTTTTGTACTTCGGCATGTTTCCTGAAGACTATTCAATCAGCCGTGACAGGCTGATTCGGTTGTGGTTAGCCGAAGGATTTGTGGTGCCAGGAAGAGGACAAACAATGGAGGAGGCTGCAGAGAGTTATCTGAATGAACTCATTGGAAGAAATTTGGTACATGCTACCACAAGGGAGATTAATGGTCGAGTAAGGAGCTGTCGTGTTTTGAACCTTGTTCGAGAGTTCATCATTTCAAAAGCAGAGAACTTCATCACGATTTTGGATCCAAACTGCCCTATTTCTACTCGTTCAAGTGAGAAAATTCGACGTCTTTCAATTCAAAATGTCAATATCAATTTATTAAGGGGTAGAGACTTTAGTCATGTTCGTACCTTACTAGTGTTTGGAGAGGAGTGTTCCCCTTCTGAGCTTGACAATGTGTTTAAGACCTCTAAGTTTTTAAGGGTGTTAGATTTTCAAGGTGTACCTTTGAAGAACTTTCCTGACACTGTTATTGGTCTCCGCCTCTTGAGGTACTTATGTCTGAGCCAAACTGATATTAAATCAGTTCCAAAGTCCATTGGCAATCTTGCATTCTTGCAAACCTTAGATCTTAAGCGCACACAGGTGACCAACTTACCAAAACAGATTTATGCGCTCCAGGATATGCGTCATCTTTTAGTTTATAGCTGTGATGTGACTTCTGGAGTGGCACAAGGAGTTCAGATTTCTGCAGGCAATATTGGAGCTTTATCATCCCTACAGAAAAAAGTAGTCCTTAAAGCCTTGGGAGAGTTAATTGCTCTCCGTAAGTTGGGGCTGGTAGACCTTGAAAGAGAACATGGAAGAGAATTGTGTTGTTCCATTCAAAAAATGCCAGAGCTTTCAACACTTGATGTACGATCAACAAGCGAAGATGAGTATCTTGATTTGGATCAATTGAACTTCCCTCCCGGGCCTCCCCGCTATCTCCAACGTCTCTGTTTGACAGGGCGCCTAGAAAGTCTACCACAGTGGATTCCCCAGCTTCACAGTCTAGCTCGGATTGCTTTGAAGTGGTCTAAACTGAATGCTGATGTCAACTCACTAGAGGCGCTCCAAGATTTGCCTAATCTGGTGGAGCTAGAGTTGGTGGGCTATTACACTGGGGATTCAATGAAGTTCAAAGCCAAAAAGTTCAAGAAACTGAAGGTATTACGTATTGAGCAACTTGATCAGCTAGATATGGTGGTAGTCGAGGATGGAGCAATGCCCGAGCTTAAGAAGTTAACATTGTCTAGATGTCAGAATCTGAAGTTACTTCCATTTGGCATTGATAGGCTTATCTTGCTTGAAGAATTGATTCTGTATGACATGCCCGAAGAATTCATTGCTAGGCTTCAGAAGGATAGCGAAGACCGTTACTTGGTTGAGAATCTTCGAGTCATTCAATCTTTCTATTCTGGGAGCACTGGATGCCAAAATCTTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

883

Amino Acids

100.3

Weight (kDa)

7.52

Isoelectric Point (pI)

51.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 31 - 95 7.3e-07 Rx N-terminal domain
NB-ARC PF00931 169 - 228 3.9e-10 NB-ARC domain
nSTAND_NTPase5 PF25199 174 - 287 2.6e-07 Novel STAND NTPase 5
NB-ARC PF00931 239 - 319 3.8e-15 NB-ARC domain
WHD_DRP PF23559 405 - 476 8.5e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 521 - 840 3.1e-40 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1510
AccB1I GGYRCC 2 cut(s) 1282, 2172
AccB7I CCANNNNNTGG 1 cut(s) 226
AccI GTMKAC 2 cut(s) 2004, 2185
AccII CGCG 1 cut(s) 333
AciI CCGC 5 cut(s) 183, 585, 897, 1702, 2143
AclWI GGATC 6 cut(s) 460, 671, 991, 1457, 1470, 2121
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 3 cut(s) 1348, 1500, 2543
AcuI CTGAAG 6 cut(s) 740, 1208, 1242, 2387, 2492, 2543
AcyI GRCGYC 2 cut(s) 1507, 2173
AfaI GTAC 6 cut(s) 1205, 1356, 1570, 1665, 1715, 2079
AfiI CCNNNNNNNGG 6 cut(s) 153, 226, 447, 1292, 1993, 2584
AflII CTTAAG 2 cut(s) 1793, 2438
AflIII ACRYGT 1 cut(s) 669
AhlI ACTAGT 1 cut(s) 1576
AjiI CACGTC 1 cut(s) 670
AjnI CCWGG 5 cut(s) 119, 580, 808, 1285, 1839
AleI CACNNNNGTG 2 cut(s) 605, 1805
Alw21I GWGCWC 1 cut(s) 2630
Alw26I GTCTC 4 cut(s) 927, 1545, 1703, 2162
AlwI GGATC 6 cut(s) 460, 671, 991, 1457, 1470, 2121
AlwNI CAGNNNCTG 2 cut(s) 310, 2470
Ama87I CYCGRG 2 cut(s) 2132, 2432
AoxI GGCC 4 cut(s) 36, 499, 677, 2135
ApeKI GCWGC 5 cut(s) 180, 186, 310, 1156, 1313
ApoI RAATTY 3 cut(s) 1348, 1500, 2543
AseI ATTAAT 1 cut(s) 1377
Asp700I GAANNNNTTC 1 cut(s) 2520
AspLEI GCGC 4 cut(s) 1800, 1837, 2175, 2275
AspS9I GGNCC 4 cut(s) 82, 578, 634, 2135
AsuC2I CCSGG 2 cut(s) 2133, 2134
AsuHPI GGTGA 1 cut(s) 1819
AvaI CYCGRG 2 cut(s) 2132, 2432
AvaII GGWCC 3 cut(s) 82, 578, 634
BaeI ACNNNNGTAYC 2 cut(s) 1705, 1738
BamHI GGATCC 1 cut(s) 1462
BanI GGYRCC 2 cut(s) 1282, 2172
BauI CACGAG 1 cut(s) 858
BbsI GAAGAC 2 cut(s) 1230, 2580
Bbv12I GWGCWC 1 cut(s) 2630
BbvI GCAGC 5 cut(s) 192, 198, 297, 1168, 1300
BccI CCATC 2 cut(s) 1191, 2414
BceAI ACGGC 3 cut(s) 692, 1131, 1224
BciT130I CCWGG 5 cut(s) 121, 582, 810, 1287, 1841
BclI TGATCA 1 cut(s) 2392
BcnI CCSGG 2 cut(s) 2133, 2134
BcoDI GTCTC 4 cut(s) 927, 1545, 1703, 2162
BcuI ACTAGT 1 cut(s) 1576
BfmI CTRYAG 4 cut(s) 954, 1314, 1911, 1940
BfoI RGCGCY 2 cut(s) 2176, 2276
BfrI CTTAAG 2 cut(s) 1793, 2438
BglI GCCNNNNNGGC 1 cut(s) 44
BglII AGATCT 2 cut(s) 769, 1789
BisI GCNGC 7 cut(s) 181, 184, 187, 311, 586, 1157, 1314
BlsI GCNGC 7 cut(s) 182, 185, 188, 312, 587, 1158, 1315
Bme1390I CCNGG 7 cut(s) 121, 582, 810, 1287, 1841, 2133, 2134
Bme18I GGWCC 3 cut(s) 82, 578, 634
BmeT110I CYCGRG 2 cut(s) 2132, 2432
BmgBI CACGTC 1 cut(s) 670
BmgT120I GGNCC 4 cut(s) 82, 578, 634, 2135
BmiI GGNNCC 6 cut(s) 84, 510, 589, 1284, 1464, 2174
BmrFI CCNGG 7 cut(s) 121, 582, 810, 1287, 1841, 2133, 2134
BmrI ACTGGG 1 cut(s) 2334
BmsI GCATC 6 cut(s) 129, 235, 702, 724, 1180, 2624
BmuI ACTGGG 1 cut(s) 2334
BoxI GACNNNNGTC 2 cut(s) 1128, 1557
BpiI GAAGAC 2 cut(s) 1230, 2580
BpmI CTGGAG 3 cut(s) 1056, 1823, 1905
BpuEI CTTGAG 6 cut(s) 272, 443, 500, 524, 542, 1729
BpuMI CCSGG 2 cut(s) 2133, 2134
BsaAI YACGTR 1 cut(s) 2378
BsaBI GATNNNNATC 2 cut(s) 2100, 2112
BsaHI GRCGYC 2 cut(s) 1507, 2173
BsaI GGTCTC 2 cut(s) 927, 1703
BsaJI CCNNGG 5 cut(s) 630, 809, 1102, 1965, 2132
BsaXI ACNNNNNCTCC 4 cut(s) 1384, 1414, 2291, 2321
Bsc4I CCNNNNNNNGG 6 cut(s) 153, 226, 447, 1292, 1993, 2584
Bse1I ACTGG 3 cut(s) 1073, 2329, 2635
Bse3DI GCAATG 2 cut(s) 2433, 2547
Bse8I GATNNNNATC 2 cut(s) 2100, 2112
BseBI CCWGG 5 cut(s) 121, 582, 810, 1287, 1841
BseDI CCNNGG 5 cut(s) 630, 809, 1102, 1965, 2132
BseGI GGATG 5 cut(s) 144, 250, 1934, 2425, 2639
BseJI GATNNNNATC 2 cut(s) 2100, 2112
BseLI CCNNNNNNNGG 6 cut(s) 153, 226, 447, 1292, 1993, 2584
BseMI GCAATG 2 cut(s) 2433, 2547
BseMII CTCAG 4 cut(s) 281, 317, 1596, 1715
BseNI ACTGG 3 cut(s) 1073, 2329, 2635
BseRI GAGGAG 3 cut(s) 521, 1322, 1604
BseXI GCAGC 5 cut(s) 192, 198, 297, 1168, 1300
BseYI CCCAGC 1 cut(s) 2202
BsgI GTGCAG 1 cut(s) 296
Bsh1236I CGCG 1 cut(s) 333
Bsh1285I CGRYCG 1 cut(s) 64
BshFI GGCC 4 cut(s) 38, 501, 679, 2137
BshNI GGYRCC 2 cut(s) 1282, 2172
BsiEI CGRYCG 1 cut(s) 64
BsiHKAI GWGCWC 1 cut(s) 2630
BsiHKCI CYCGRG 2 cut(s) 2132, 2432
BsiSI CCGG 2 cut(s) 591, 2133
BslFI GGGAC 1 cut(s) 170
BslI CCNNNNNNNGG 6 cut(s) 153, 226, 447, 1292, 1993, 2584
BsmAI GTCTC 4 cut(s) 927, 1545, 1703, 2162
BsmBI CGTCTC 1 cut(s) 2162
BsmFI GGGAC 1 cut(s) 170
BsmI GAATGC 2 cut(s) 1772, 2254
BsnI GGCC 4 cut(s) 38, 501, 679, 2137
Bso31I GGTCTC 2 cut(s) 927, 1703
BsoBI CYCGRG 2 cut(s) 2132, 2432
Bsp1286I GDGCHC 1 cut(s) 2630
Bsp19I CCATGG 1 cut(s) 630
Bsp68I TCGCGA 1 cut(s) 333
BspACI CCGC 5 cut(s) 183, 585, 897, 1702, 2143
BspANI GGCC 4 cut(s) 38, 501, 679, 2137
BspCNI CTCAG 4 cut(s) 280, 316, 1597, 1716
BspFNI CGCG 1 cut(s) 333
BspLI GGNNCC 6 cut(s) 84, 510, 589, 1284, 1464, 2174
BspMAI CTGCAG 3 cut(s) 958, 1318, 1915
BspPI GGATC 6 cut(s) 460, 671, 991, 1457, 1470, 2121
BspT107I GGYRCC 2 cut(s) 1282, 2172
BspTI CTTAAG 2 cut(s) 1793, 2438
BspTNI GGTCTC 2 cut(s) 927, 1703
BsrDI GCAATG 2 cut(s) 2433, 2547
BsrI ACTGG 3 cut(s) 1073, 2329, 2635
BssECI CCNNGG 5 cut(s) 630, 809, 1102, 1965, 2132
BssNI GRCGYC 2 cut(s) 1507, 2173
BssSI CACGAG 1 cut(s) 858
BssT1I CCWWGG 3 cut(s) 630, 1102, 1965
Bst2BI CACGAG 1 cut(s) 858
Bst2UI CCWGG 5 cut(s) 121, 582, 810, 1287, 1841
Bst4CI ACNGT 6 cut(s) 534, 604, 1690, 2193, 2213, 2579
Bst6I CTCTTC 5 cut(s) 159, 446, 975, 1285, 2020
BstACI GRCGYC 2 cut(s) 1507, 2173
BstAFI CTTAAG 2 cut(s) 1793, 2438
BstAPI GCANNNNNTGC 1 cut(s) 2634
BstBAI YACGTR 1 cut(s) 2378
BstC8I GCNNGC 3 cut(s) 144, 1053, 1915
BstDEI CTNAG 6 cut(s) 267, 303, 1605, 1632, 1724, 1786
BstDSI CCRYGG 1 cut(s) 630
BstEII GGTNACC 1 cut(s) 1807
BstF5I GGATG 5 cut(s) 144, 250, 1934, 2425, 2639
BstFNI CGCG 1 cut(s) 333
BstH2I RGCGCY 2 cut(s) 2176, 2276
BstHHI GCGC 4 cut(s) 1800, 1837, 2175, 2275
BstMAI GTCTC 4 cut(s) 927, 1545, 1703, 2162
BstMCI CGRYCG 1 cut(s) 64
BstMWI GCNNNNNNNGC 8 cut(s) 11, 44, 186, 591, 1034, 2225, 2506, 2634
BstNI CCWGG 5 cut(s) 121, 582, 810, 1287, 1841
BstNSI RCATGY 4 cut(s) 1171, 1216, 1361, 2536
BstPAI GACNNNNGTC 2 cut(s) 1128, 1557
BstPI GGTNACC 1 cut(s) 1807
BstSCI CCNGG 7 cut(s) 119, 580, 808, 1285, 1839, 2131, 2132
BstSFI CTRYAG 4 cut(s) 954, 1314, 1911, 1940
BstSNI TACGTA 1 cut(s) 2378
BstUI CGCG 1 cut(s) 333
BstV1I GCAGC 5 cut(s) 192, 198, 297, 1168, 1300
BstV2I GAAGAC 2 cut(s) 1230, 2580
BstX2I RGATCY 4 cut(s) 769, 996, 1462, 1789
BstXI CCANNNNNNTGG 1 cut(s) 1080
BstYI RGATCY 4 cut(s) 769, 996, 1462, 1789
BsuRI GGCC 4 cut(s) 38, 501, 679, 2137
BtgI CCRYGG 1 cut(s) 630
BtgZI GCGATG 1 cut(s) 62
BtrI CACGTC 1 cut(s) 670
BtsCI GGATG 5 cut(s) 144, 250, 1934, 2425, 2639
BtsIMutI CAGTG 4 cut(s) 1686, 2198, 2322, 2628
BtuMI TCGCGA 1 cut(s) 333
Cac8I GCNNGC 3 cut(s) 144, 1053, 1915
CaiI CAGNNNCTG 2 cut(s) 310, 2470
CfoI GCGC 4 cut(s) 1800, 1837, 2175, 2275
Cfr13I GGNCC 4 cut(s) 82, 578, 634, 2135
Cfr9I CCCGGG 1 cut(s) 2132
CseI GACGC 1 cut(s) 1838
Csp6I GTAC 6 cut(s) 1204, 1355, 1569, 1664, 1714, 2078
CviQI GTAC 6 cut(s) 1204, 1355, 1569, 1664, 1714, 2078
DdeI CTNAG 6 cut(s) 267, 303, 1605, 1632, 1724, 1786
DinI GGCGCC 1 cut(s) 2174
EaeI YGGCCR 1 cut(s) 36
Eam1104I CTCTTC 5 cut(s) 159, 446, 975, 1285, 2020
EarI CTCTTC 5 cut(s) 159, 446, 975, 1285, 2020
EciI GGCGGA 1 cut(s) 1691
Eco105I TACGTA 1 cut(s) 2378
Eco130I CCWWGG 3 cut(s) 630, 1102, 1965
Eco147I AGGCCT 1 cut(s) 501
Eco31I GGTCTC 2 cut(s) 927, 1703
Eco47I GGWCC 3 cut(s) 82, 578, 634
Eco57I CTGAAG 6 cut(s) 740, 1208, 1242, 2387, 2492, 2543
Eco88I CYCGRG 2 cut(s) 2132, 2432
Eco91I GGTNACC 1 cut(s) 1807
EcoO109I RGGNCCY 2 cut(s) 82, 578
EcoO65I GGTNACC 1 cut(s) 1807
EcoRI GAATTC 1 cut(s) 2543
EcoRII CCWGG 5 cut(s) 119, 580, 808, 1285, 1839
EcoT14I CCWWGG 3 cut(s) 630, 1102, 1965
EcoT22I ATGCAT 2 cut(s) 717, 1173
EgeI GGCGCC 1 cut(s) 2174
EheI GGCGCC 1 cut(s) 2174
ErhI CCWWGG 3 cut(s) 630, 1102, 1965
Esp3I CGTCTC 1 cut(s) 2162
FalI AAGNNNNNCTT 4 cut(s) 214, 246, 1941, 1973
FaqI GGGAC 1 cut(s) 170
FauI CCCGC 2 cut(s) 890, 2150
FbaI TGATCA 1 cut(s) 2392
FblI GTMKAC 2 cut(s) 2004, 2185
Fnu4HI GCNGC 7 cut(s) 181, 184, 187, 311, 586, 1157, 1314
FokI GGATG 5 cut(s) 151, 257, 1921, 2432, 2646
Fsp4HI GCNGC 7 cut(s) 181, 184, 187, 311, 586, 1157, 1314
GlaI GCGC 4 cut(s) 1799, 1836, 2174, 2274
GluI GCNGC 7 cut(s) 181, 184, 187, 311, 586, 1157, 1314
GsaI CCCAGC 1 cut(s) 2206
GsuI CTGGAG 3 cut(s) 1056, 1823, 1905
HaeII RGCGCY 2 cut(s) 2176, 2276
HaeIII GGCC 4 cut(s) 38, 501, 679, 2137
HapII CCGG 2 cut(s) 591, 2133
HgaI GACGC 1 cut(s) 1838
HhaI GCGC 4 cut(s) 1800, 1837, 2175, 2275
Hin1I GRCGYC 2 cut(s) 1507, 2173
Hin6I GCGC 4 cut(s) 1798, 1835, 2173, 2273
HinP1I GCGC 4 cut(s) 1798, 1835, 2173, 2273
HincII GTYRAC 2 cut(s) 2260, 2448
HindII GTYRAC 2 cut(s) 2260, 2448
HpaI GTTAAC 1 cut(s) 2448
HpaII CCGG 2 cut(s) 591, 2133
HphI GGTGA 1 cut(s) 1819
Hpy166II GTNNAC 6 cut(s) 968, 1664, 2005, 2186, 2260, 2448
Hpy8I GTNNAC 6 cut(s) 968, 1664, 2005, 2186, 2260, 2448
Hpy99I CGWCG 3 cut(s) 320, 671, 1509
HpyCH4III ACNGT 6 cut(s) 534, 604, 1690, 2193, 2213, 2579
HpyCH4IV ACGT 5 cut(s) 318, 669, 1507, 2155, 2377
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 44, 186, 591, 1034, 2225, 2506, 2634
HpyF3I CTNAG 6 cut(s) 267, 303, 1605, 1632, 1724, 1786
HpySE526I ACGT 5 cut(s) 318, 669, 1507, 2155, 2377
Hsp92I GRCGYC 2 cut(s) 1507, 2173
HspAI GCGC 4 cut(s) 1798, 1835, 2173, 2273
KasI GGCGCC 1 cut(s) 2172
Ksp22I TGATCA 1 cut(s) 2392
KspAI GTTAAC 1 cut(s) 2448
LmnI GCTCC 9 cut(s) 508, 593, 1392, 1842, 1925, 2280, 2299, 2423, 2625
Lsp1109I GCAGC 5 cut(s) 192, 198, 297, 1168, 1300
LweI GCATC 6 cut(s) 129, 235, 702, 724, 1180, 2624
MaeII ACGT 5 cut(s) 318, 669, 1507, 2155, 2377
MaeIII GTNAC 9 cut(s) 319, 672, 1089, 1163, 1241, 1807, 1876, 2475, 2579
MfeI CAATTG 1 cut(s) 2117
MflI RGATCY 4 cut(s) 769, 996, 1462, 1789
MhlI GDGCHC 1 cut(s) 2630
Mly113I GGCGCC 1 cut(s) 2173
MlyI GAGTC 5 cut(s) 152, 516, 875, 1118, 2610
MmeI TCCRAC 2 cut(s) 57, 2176
Mph1103I ATGCAT 2 cut(s) 717, 1173
MroXI GAANNNNTTC 1 cut(s) 2520
MslI CAYNNNNRTG 3 cut(s) 105, 605, 1805
MspA1I CMGCKG 2 cut(s) 183, 1037
MspCI CTTAAG 2 cut(s) 1793, 2438
MspI CCGG 2 cut(s) 591, 2133
MspR9I CCNGG 7 cut(s) 121, 582, 810, 1287, 1841, 2133, 2134
MunI CAATTG 1 cut(s) 2117
Mva1269I GAATGC 2 cut(s) 1772, 2254
MvaI CCWGG 5 cut(s) 121, 582, 810, 1287, 1841
MvnI CGCG 1 cut(s) 333
MwoI GCNNNNNNNGC 8 cut(s) 11, 44, 186, 591, 1034, 2225, 2506, 2634
NarI GGCGCC 1 cut(s) 2173
NciI CCSGG 2 cut(s) 2133, 2134
NcoI CCATGG 1 cut(s) 630
NlaIV GGNNCC 6 cut(s) 84, 510, 589, 1284, 1464, 2174
NmeAIII GCCGAG 1 cut(s) 64
NmuCI GTSAC 4 cut(s) 672, 1241, 1807, 1876
NruI TCGCGA 1 cut(s) 333
NsiI ATGCAT 2 cut(s) 717, 1173
NspI RCATGY 4 cut(s) 1171, 1216, 1361, 2536
OliI CACNNNNGTG 2 cut(s) 605, 1805
PceI AGGCCT 1 cut(s) 501
PctI GAATGC 2 cut(s) 1772, 2254
PdmI GAANNNNTTC 1 cut(s) 2520
PfeI GAWTC 9 cut(s) 271, 413, 548, 1252, 2197, 2330, 2467, 2521, 2593
PflMI CCANNNNNTGG 1 cut(s) 226
PfoI TCCNGGA 1 cut(s) 1839
PkrI GCNGC 7 cut(s) 182, 185, 188, 312, 587, 1158, 1315
PleI GAGTC 5 cut(s) 152, 516, 874, 1118, 2609
PluTI GGCGCC 1 cut(s) 2176
PpsI GAGTC 5 cut(s) 152, 516, 874, 1118, 2609
Ppu21I YACGTR 1 cut(s) 2378
PpuMI RGGWCCY 2 cut(s) 82, 578
PshAI GACNNNNGTC 2 cut(s) 1128, 1557
PshBI ATTAAT 1 cut(s) 1377
Psp5II RGGWCCY 2 cut(s) 82, 578
Psp6I CCWGG 5 cut(s) 119, 580, 808, 1285, 1839
PspEI GGTNACC 1 cut(s) 1807
PspFI CCCAGC 1 cut(s) 2202
PspGI CCWGG 5 cut(s) 119, 580, 808, 1285, 1839
PspN4I GGNNCC 6 cut(s) 84, 510, 589, 1284, 1464, 2174
PspPI GGNCC 4 cut(s) 82, 578, 634, 2135
PspPPI RGGWCCY 2 cut(s) 82, 578
PstI CTGCAG 3 cut(s) 958, 1318, 1915
PstNI CAGNNNCTG 2 cut(s) 310, 2470
PsuI RGATCY 4 cut(s) 769, 996, 1462, 1789
PvuII CAGCTG 1 cut(s) 1037
RruI TCGCGA 1 cut(s) 333
RsaI GTAC 6 cut(s) 1205, 1356, 1570, 1665, 1715, 2079
RsaNI GTAC 6 cut(s) 1204, 1355, 1569, 1664, 1714, 2078
RseI CAYNNNNRTG 3 cut(s) 105, 605, 1805
SatI GCNGC 7 cut(s) 181, 184, 187, 311, 586, 1157, 1314
Sau96I GGNCC 4 cut(s) 82, 578, 634, 2135
SchI GAGTC 5 cut(s) 152, 516, 875, 1118, 2610
ScrFI CCNGG 7 cut(s) 121, 582, 810, 1287, 1841, 2133, 2134
SduI GDGCHC 1 cut(s) 2630
SfaNI GCATC 6 cut(s) 129, 235, 702, 724, 1180, 2624
SfcI CTRYAG 4 cut(s) 954, 1314, 1911, 1940
SfoI GGCGCC 1 cut(s) 2174
SinI GGWCC 3 cut(s) 82, 578, 634
SmaI CCCGGG 1 cut(s) 2134
SmiMI CAYNNNNRTG 3 cut(s) 105, 605, 1805
SmlI CTYRAG 8 cut(s) 251, 422, 479, 503, 557, 1708, 1793, 2438
SmoI CTYRAG 8 cut(s) 251, 422, 479, 503, 557, 1708, 1793, 2438
SnaBI TACGTA 1 cut(s) 2378
SpeI ACTAGT 1 cut(s) 1576
SseBI AGGCCT 1 cut(s) 501
SsiI CCGC 5 cut(s) 183, 585, 897, 1702, 2143
SspDI GGCGCC 1 cut(s) 2172
SspI AATATT 1 cut(s) 1921
StuI AGGCCT 1 cut(s) 501
StyD4I CCNGG 7 cut(s) 119, 580, 808, 1285, 1839, 2131, 2132
StyI CCWWGG 3 cut(s) 630, 1102, 1965
TaaI ACNGT 6 cut(s) 534, 604, 1690, 2193, 2213, 2579
TaiI ACGT 5 cut(s) 321, 672, 1510, 2158, 2380
TaqI TCGA 7 cut(s) 666, 1136, 1384, 1417, 1504, 2415, 2599
TaqII GACCGA 1 cut(s) 78
TatI WGTACW 1 cut(s) 1203
TauI GCSGC 2 cut(s) 186, 588
TfiI GAWTC 9 cut(s) 271, 413, 548, 1252, 2197, 2330, 2467, 2521, 2593
TscAI CASTG 4 cut(s) 1693, 2198, 2329, 2635
TseFI GTSAC 4 cut(s) 672, 1241, 1807, 1876
TseI GCWGC 5 cut(s) 180, 186, 310, 1156, 1313
Tsp45I GTSAC 4 cut(s) 672, 1241, 1807, 1876
TspGWI ACGGA 1 cut(s) 1976
TspMI CCCGGG 1 cut(s) 2132
TspRI CASTG 4 cut(s) 1693, 2198, 2329, 2635
Van91I CCANNNNNTGG 1 cut(s) 226
Vha464I CTTAAG 2 cut(s) 1793, 2438
VpaK11BI GGWCC 3 cut(s) 82, 578, 634
VspI ATTAAT 1 cut(s) 1377
XapI RAATTY 3 cut(s) 1348, 1500, 2543
XbaI TCTAGA 1 cut(s) 2456
XceI RCATGY 4 cut(s) 1171, 1216, 1361, 2536
XmaI CCCGGG 1 cut(s) 2132
XmiI GTMKAC 2 cut(s) 2004, 2185
XmnI GAANNNNTTC 1 cut(s) 2520
ZraI GACGTC 1 cut(s) 1508
Zsp2I ATGCAT 2 cut(s) 717, 1173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.