RchiOBHm_Chr4g0399061

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000865
Physical Location & Seq
Reverse (-)
16163005 .. 16166711
3707 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37120

Sequence Viewer

Length: 2880 bp
ATGGCTGATCTAGCCGTTTCTGTAGCAATATTATTAGTTGAGAAGGTGATAAGCTCTTTGGACCAAAAGATCACTAGTAATGTCCATGGTGATGTTGAAAAGGCCTGGAAATGTTTGAGGAGGATGCAAGCCTATCTAAGGGACTCGGAAGAGATTGACGTAGGTAGTCAAATGTTTCAAAATCGTGTCAAAGAGGTCCAAAGTTTAGCTTATGAAATTGAGGATGCTATTGATAAGTTTATGGTTCAGATTCCACATCACTTCCACAGCAATATGTTCTCTGAGGCGATTCACGACATTGCCTACTTTTTCACAGAAAGAAGAGCAATAAGTGAGTTCTCCTCAAAAATTAAAGATATTGAAACGAGAGTTGGTAGCTTTTCAGCCTTGGATTCACTACTTCTTGAGCCAACAAGTTCTAGCACAAGAACAGAAGAAAACCTGACCCACCAAGTTCTCGAGGAAGATGAGGTAGTGGGCTTTGAAGAGCGTAAAGGAACACTCCTCAAACAGTTGACAGAAGCAAATTCAAAGCTCTTGACGATTTTGATTGTAGGTCCAGGCGGCTCCGGCAAAACCACAATCGTGAAGAATGTTTATGACAGTAAACTGGTACAAGGATTTTTTGATTGCCATTCTTGGATTGATGTGTCACGTCCTCCTAAACTTGGAAAGCTCTTGCGTAATATGTTAATCGATTTCAGACTGAAAAAGAATAACAGCGCTTGGAGTAAATATGAAGCTGTTAGACAGATTGTTCCAAGAGCGGGCATGAGACTATTTCCGCAGCAAAAACTGAAAAGACAAAAACCAGTTCATGATGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGCAGCTGAAAATCCAAAAGCAGAATTGAATAATTTTCTGAAAGCTAAGAGGTTTCTGCTTGTGTTAGATAATGTTTGGAGAGTACAAGATCTGGAATCCATTGTAAATGCTCTACCAAAAGGATTGCCTGGGAGTAAAATAATCATAACTACACGGAAGTCTGATGTAGCTCCGGTTGCCACATATATCCATGATTTGAGTTGTGGTTTGTCATGGAAATATGCCTGGTATCTGTTCTGCAAGAAGGCTTTTCAAAGTAGTGACGAAAATTGTCCGCGCGAGCTCGAGCACTGGGCTAAAAAAATCCTGAAGAAGTGTGAAGGCTTGCCCCTTGCTATTTCAGCTGTTGGTACTTTGCTCTGGAAGAAGCCACAAACTCCAGTTGAGTGGAAGAAGTTACATGAAAGCCTTGGATCTGAAATTAGACCCGATTCTAGTCTTTCTGTTATTAGCCGCATTTTGCAGCCCAGTTATATGGATCTTCCAAGCCATCTGAAAACTTGTTTCTTATACTTTGGCATGTTTCCAGAAGACTATTTAATCAGTCGCGAGAAGCTGACTCGGTTGTGGTTAGCCGAAGGGTTTGTGATGCCAGGAAGAGGAAAAACAATGGAGGAAGTTGCAGAGAGTTATCTCAATGAACTCATTGCAAGAAACTTGGTTAATGCCACCAGAAGAGAGATAGATGGTCGAGTGAGGAGTTGTCGTGTTTTAAGCCTTGTCAGAGAGTTCATCATTACCAAGGCAGAAAACTTCATCACTGTTTTGGAGCCCAACTGCAGTACTACAACGTCAGGTGAGAAAATTCGTCGCCTTTCTGTTCAAAATGTCGAAATCAATTTGCTATTAAGAGATAGGGACTTGAGTCACATTCGTACCTTGCTAGTGTTTGGGCAGGCATGTTCCCCTTCTGATCTTGAAATGGTACTTAAAACCTCTAAATTTTTGAGGGTGTTTGATCTTCAAGGAGTACCTTTGAAGATTTTTCCCAAAAGTATAATTGGTCTCCGCCTTTTGAGGTACTTATGTCTGAGGCAAACTAAAATTAGATCAGTTCCAAAGTCCATAAAAAATCTTGCATTCTTGCAAACTTTAGATCTGAGGTCCACCAAGGTGACAAAATTACCAAAAACGATCTACAAATTCCAGAATATGCGTCATCTTTTAGCTTATAGCTGTGATATGAAATCTGGAGTGGCACGAGGAGTGGACGTGTCTGCGGGCAATATTGGATCGTTATCTTCCATACAGAAGTTGTTACTAGTCAAGGTGCAAAAAAACAGAAAAGCCATAAAAGCCTTGAGAGAGTTGATTGGTCTCCGGAAGTTGGGGCTGGTCGACCTTGGAAGAGAACATGGAAGAGAATTGTGTTGTTCCATTCAAAAGATGTCAGAGCTTTCAACACTTGAGGTACGATCAACAAGCGAGGAGGAGTATCTCGATTTGGATCATATGAATTCCCCCCCTGGTCCTCCTCTCTATCTCCAACGTCTATCTTTGACAGGGCGCTTAAAAATTCTACCGCAGTGGATAGCGCAGCTTCATAGTCTGATTAGGATTGCTTTGAAGGGTTCAAAACTGAATGCTGGTGCCAACATTGTTGAGGTCCTTCAAGATTTGCCTAATCTGATGGAACTAGAATTGGTTGACTCATTCACTGGGGTATCTTTGATATTCAAAGCTGAAAAGTTCAAGAAACTAAAGGCCTTGCGTATTGAGAAATTTGATCAGCTAGATTTGATGGTCGTCGAGGGTGGAGCAATGCCTCAGCTCAAGAAGTTAACCATGTCTAAATGCCAGAATCTGAAGTTACTTCCATTTGGCATTGATCATCTAATCTTCCTCCAAGAACTGCTCTTGATTGACATGCACCATGAATTCCTTGCTAGGCTACAGAAGGATAGCGAAGACCGTTACTTGGTTGAGAATATTCGAGTTATCCATTCCTTCTCTGGGAGCTACCAATCTTACACTGGATTCCAAAATCTCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

959

Amino Acids

109.55

Weight (kDa)

8.54

Isoelectric Point (pI)

50.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 12 - 88 1.3e-12 Rx N-terminal domain
NB-ARC PF00931 162 - 242 7.6e-12 NB-ARC domain
NB-ARC PF00931 287 - 383 4.4e-16 NB-ARC domain
WHD_DRP PF23559 469 - 540 1e-19 Disease resistance protein Winged helix domain
LRR_14 PF23598 585 - 913 3.7e-42 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 2474
AccBSI CCGCTC 1 cut(s) 767
AccI GTMKAC 1 cut(s) 2223
AccII CGCG 3 cut(s) 1162, 1164, 1434
AccIII TCCGGA 1 cut(s) 2205
AciI CCGC 8 cut(s) 564, 767, 785, 1160, 1339, 1894, 2105, 2408
AclWI GGATC 4 cut(s) 1306, 1371, 2125, 2340
AcsI RAATTY 8 cut(s) 526, 1689, 1826, 2027, 2341, 2400, 2606, 2762
AcuI CTGAAG 2 cut(s) 1214, 2711
AfaI GTAC 9 cut(s) 615, 969, 1237, 1669, 1762, 1812, 1857, 1907, 2298
AfeI AGCGCT 1 cut(s) 724
AfiI CCNNNNNNNGG 7 cut(s) 138, 668, 767, 1484, 2212, 2803, 2838
AflIII ACRYGT 1 cut(s) 2097
AhlI ACTAGT 2 cut(s) 74, 2146
AjiI CACGTC 2 cut(s) 656, 2098
AjnI CCWGG 6 cut(s) 104, 559, 1012, 1109, 1477, 2350
AjuI GAANNNNNNNTTGG 2 cut(s) 354, 386
AleI CACNNNNGTG 2 cut(s) 584, 1997
Alw21I GWGCWC 2 cut(s) 1170, 1176
Alw26I GTCTC 3 cut(s) 769, 1895, 2207
AlwI GGATC 4 cut(s) 1306, 1371, 2125, 2340
AlwNI CAGNNNCTG 1 cut(s) 2689
Ama87I CYCGRG 2 cut(s) 458, 1169
Aor13HI TCCGGA 1 cut(s) 2205
Aor51HI AGCGCT 1 cut(s) 724
AoxI GGCC 2 cut(s) 102, 2589
ApeKI GCWGC 4 cut(s) 787, 887, 1348, 2422
ApoI RAATTY 8 cut(s) 526, 1689, 1826, 2027, 2341, 2400, 2606, 2762
AspLEI GCGC 4 cut(s) 725, 1164, 2394, 2422
AspS9I GGNCC 6 cut(s) 61, 196, 557, 1989, 2354, 2491
AsuHPI GGTGA 4 cut(s) 58, 101, 1694, 2011
AvaI CYCGRG 2 cut(s) 458, 1169
AvaII GGWCC 6 cut(s) 61, 196, 557, 1989, 2354, 2491
BaeI ACNNNNGTAYC 6 cut(s) 1744, 1777, 1897, 1930, 2532, 2565
BanI GGYRCC 1 cut(s) 2474
BanII GRGCYC 2 cut(s) 1170, 1659
BauI CACGAG 1 cut(s) 2085
BbsI GAAGAC 2 cut(s) 1422, 2799
Bbv12I GWGCWC 2 cut(s) 1170, 1176
BbvCI CCTCAGC 1 cut(s) 2652
BbvI GCAGC 4 cut(s) 799, 899, 1360, 2434
BccI CCATC 4 cut(s) 1383, 1565, 2509, 2620
BciT130I CCWGG 6 cut(s) 106, 561, 1014, 1111, 1479, 2352
BclI TGATCA 2 cut(s) 2611, 2713
BcoDI GTCTC 3 cut(s) 769, 1895, 2207
BcuI ACTAGT 2 cut(s) 74, 2146
BfaI CTAG 9 cut(s) 11, 75, 420, 1320, 1769, 2147, 2522, 2618, 2772
BfmI CTRYAG 3 cut(s) 21, 1663, 2777
BfoI RGCGCY 2 cut(s) 726, 2395
BglII AGATCT 2 cut(s) 973, 1981
BisI GCNGC 6 cut(s) 565, 788, 888, 1339, 1349, 2423
BlsI GCNGC 6 cut(s) 566, 789, 889, 1340, 1350, 2424
BmcAI AGTACT 1 cut(s) 1669
Bme1390I CCNGG 6 cut(s) 106, 561, 1014, 1111, 1479, 2352
Bme18I GGWCC 6 cut(s) 61, 196, 557, 1989, 2354, 2491
BmeT110I CYCGRG 2 cut(s) 458, 1169
BmgBI CACGTC 2 cut(s) 656, 2098
BmgT120I GGNCC 6 cut(s) 61, 196, 557, 1989, 2354, 2491
BmiI GGNNCC 3 cut(s) 568, 1656, 2476
BmrFI CCNGG 6 cut(s) 106, 561, 1014, 1111, 1479, 2352
BmrI ACTGGG 3 cut(s) 1186, 1347, 2553
BmsI GCATC 3 cut(s) 114, 214, 1464
BmuI ACTGGG 3 cut(s) 1186, 1347, 2553
BoxI GACNNNNGTC 1 cut(s) 1749
BpiI GAAGAC 2 cut(s) 1422, 2799
BplI GAGNNNNNCTC 2 cut(s) 326, 358
BpmI CTGGAG 2 cut(s) 1248, 2097
Bpu10I CCTNAGC 1 cut(s) 2652
BpuEI CTTGAG 5 cut(s) 425, 1768, 2206, 2312, 2642
Bsa29I ATCGAT 1 cut(s) 696
BsaBI GATNNNNATC 2 cut(s) 2122, 2331
BsaI GGTCTC 2 cut(s) 1895, 2207
BsaJI CCNNGG 8 cut(s) 85, 387, 1013, 1294, 1626, 1995, 2227, 2350
BsaWI WCCGGW 2 cut(s) 1057, 2205
BsaXI ACNNNNNCTCC 4 cut(s) 1576, 1606, 2082, 2112
Bsc4I CCNNNNNNNGG 7 cut(s) 138, 668, 767, 1484, 2212, 2803, 2838
Bse1I ACTGG 7 cut(s) 615, 812, 1181, 1265, 1353, 2548, 2863
Bse3DI GCAATG 3 cut(s) 297, 1530, 2652
Bse8I GATNNNNATC 2 cut(s) 2122, 2331
BseAI TCCGGA 1 cut(s) 2205
BseBI CCWGG 6 cut(s) 106, 561, 1014, 1111, 1479, 2352
BseCI ATCGAT 1 cut(s) 696
BseDI CCNNGG 8 cut(s) 85, 387, 1013, 1294, 1626, 1995, 2227, 2350
BseGI GGATG 2 cut(s) 129, 229
BseJI GATNNNNATC 2 cut(s) 2122, 2331
BseLI CCNNNNNNNGG 7 cut(s) 138, 668, 767, 1484, 2212, 2803, 2838
BseMI GCAATG 3 cut(s) 297, 1530, 2652
BseMII CTCAG 4 cut(s) 273, 1907, 1976, 2666
BseNI ACTGG 7 cut(s) 615, 812, 1181, 1265, 1353, 2548, 2863
BseRI GAGGAG 8 cut(s) 133, 331, 494, 1597, 2103, 2327, 2330, 2349
BseXI GCAGC 4 cut(s) 799, 899, 1360, 2434
Bsh1236I CGCG 3 cut(s) 1162, 1164, 1434
BshFI GGCC 2 cut(s) 104, 2591
BshNI GGYRCC 1 cut(s) 2474
BshVI ATCGAT 1 cut(s) 696
BsiHKAI GWGCWC 2 cut(s) 1170, 1176
BsiHKCI CYCGRG 2 cut(s) 458, 1169
BsiSI CCGG 3 cut(s) 570, 1058, 2206
BslFI GGGAC 2 cut(s) 155, 1757
BslI CCNNNNNNNGG 7 cut(s) 138, 668, 767, 1484, 2212, 2803, 2838
BsmAI GTCTC 3 cut(s) 769, 1895, 2207
BsmFI GGGAC 2 cut(s) 155, 1757
BsmI GAATGC 2 cut(s) 1964, 2473
BsnI GGCC 2 cut(s) 104, 2591
Bso31I GGTCTC 2 cut(s) 1895, 2207
BsoBI CYCGRG 2 cut(s) 458, 1169
Bsp1286I GDGCHC 3 cut(s) 1170, 1176, 1659
Bsp13I TCCGGA 1 cut(s) 2205
Bsp19I CCATGG 1 cut(s) 85
Bsp68I TCGCGA 1 cut(s) 1434
BspACI CCGC 8 cut(s) 564, 767, 785, 1160, 1339, 1894, 2105, 2408
BspANI GGCC 2 cut(s) 104, 2591
BspCNI CTCAG 4 cut(s) 274, 1908, 1977, 2665
BspDI ATCGAT 1 cut(s) 696
BspEI TCCGGA 1 cut(s) 2205
BspFNI CGCG 3 cut(s) 1162, 1164, 1434
BspHI TCATGA 1 cut(s) 817
BspLI GGNNCC 3 cut(s) 568, 1656, 2476
BspMAI CTGCAG 1 cut(s) 1667
BspPI GGATC 4 cut(s) 1306, 1371, 2125, 2340
BspQI GCTCTTC 2 cut(s) 316, 480
BspT107I GGYRCC 1 cut(s) 2474
BspTNI GGTCTC 2 cut(s) 1895, 2207
BsrBI CCGCTC 1 cut(s) 767
BsrDI GCAATG 3 cut(s) 297, 1530, 2652
BsrI ACTGG 7 cut(s) 615, 812, 1181, 1265, 1353, 2548, 2863
BssECI CCNNGG 8 cut(s) 85, 387, 1013, 1294, 1626, 1995, 2227, 2350
BssSI CACGAG 1 cut(s) 2085
BssT1I CCWWGG 6 cut(s) 85, 387, 1294, 1626, 1995, 2227
Bst2BI CACGAG 1 cut(s) 2085
Bst2UI CCWGG 6 cut(s) 106, 561, 1014, 1111, 1479, 2352
Bst4CI ACNGT 4 cut(s) 513, 605, 1648, 2798
Bst6I CTCTTC 7 cut(s) 144, 316, 480, 1477, 1555, 2227, 2239
BstC8I GCNNGC 6 cut(s) 129, 769, 1166, 1211, 1782, 2107
BstDEI CTNAG 6 cut(s) 137, 282, 930, 1916, 1985, 2652
BstDSI CCRYGG 1 cut(s) 85
BstENI CCTNNNNNAGG 1 cut(s) 136
BstF5I GGATG 2 cut(s) 129, 229
BstFNI CGCG 3 cut(s) 1162, 1164, 1434
BstH2I RGCGCY 2 cut(s) 726, 2395
BstHHI GCGC 4 cut(s) 725, 1164, 2394, 2422
BstMAI GTCTC 3 cut(s) 769, 1895, 2207
BstMWI GCNNNNNNNGC 5 cut(s) 11, 570, 1061, 1226, 2180
BstNI CCWGG 6 cut(s) 106, 561, 1014, 1111, 1479, 2352
BstNSI RCATGY 3 cut(s) 1408, 1788, 2755
BstPAI GACNNNNGTC 1 cut(s) 1749
BstSCI CCNGG 6 cut(s) 104, 559, 1012, 1109, 1477, 2350
BstSFI CTRYAG 3 cut(s) 21, 1663, 2777
BstUI CGCG 3 cut(s) 1162, 1164, 1434
BstV1I GCAGC 4 cut(s) 799, 899, 1360, 2434
BstV2I GAAGAC 2 cut(s) 1422, 2799
BstX2I RGATCY 4 cut(s) 973, 1298, 1363, 1981
BstXI CCANNNNNNTGG 2 cut(s) 1272, 1360
BstYI RGATCY 4 cut(s) 973, 1298, 1363, 1981
Bsu15I ATCGAT 1 cut(s) 696
BsuRI GGCC 2 cut(s) 104, 2591
BsuTUI ATCGAT 1 cut(s) 696
BtgI CCRYGG 1 cut(s) 85
BtrI CACGTC 2 cut(s) 656, 2098
BtsCI GGATG 2 cut(s) 129, 229
BtsI GCAGTG 1 cut(s) 2417
BtsIMutI CAGTG 5 cut(s) 1174, 1644, 2417, 2541, 2856
BtuMI TCGCGA 1 cut(s) 1434
Cac8I GCNNGC 6 cut(s) 129, 769, 1166, 1211, 1782, 2107
CaiI CAGNNNCTG 1 cut(s) 2689
CciI TCATGA 1 cut(s) 817
CfoI GCGC 4 cut(s) 725, 1164, 2394, 2422
Cfr13I GGNCC 6 cut(s) 61, 196, 557, 1989, 2354, 2491
ClaI ATCGAT 1 cut(s) 696
CseI GACGC 1 cut(s) 2030
Csp6I GTAC 9 cut(s) 614, 968, 1236, 1668, 1761, 1811, 1856, 1906, 2297
CviQI GTAC 9 cut(s) 614, 968, 1236, 1668, 1761, 1811, 1856, 1906, 2297
DdeI CTNAG 6 cut(s) 137, 282, 930, 1916, 1985, 2652
Eam1104I CTCTTC 7 cut(s) 144, 316, 480, 1477, 1555, 2227, 2239
EarI CTCTTC 7 cut(s) 144, 316, 480, 1477, 1555, 2227, 2239
EciI GGCGGA 1 cut(s) 1883
Ecl136II GAGCTC 1 cut(s) 1168
Eco130I CCWWGG 6 cut(s) 85, 387, 1294, 1626, 1995, 2227
Eco147I AGGCCT 2 cut(s) 104, 2591
Eco24I GRGCYC 2 cut(s) 1170, 1659
Eco31I GGTCTC 2 cut(s) 1895, 2207
Eco47I GGWCC 6 cut(s) 61, 196, 557, 1989, 2354, 2491
Eco47III AGCGCT 1 cut(s) 724
Eco53kI GAGCTC 1 cut(s) 1168
Eco57I CTGAAG 2 cut(s) 1214, 2711
Eco88I CYCGRG 2 cut(s) 458, 1169
EcoICRI GAGCTC 1 cut(s) 1168
EcoNI CCTNNNNNAGG 1 cut(s) 136
EcoO109I RGGNCCY 1 cut(s) 2491
EcoRI GAATTC 2 cut(s) 2341, 2762
EcoRII CCWGG 6 cut(s) 104, 559, 1012, 1109, 1477, 2350
EcoT14I CCWWGG 6 cut(s) 85, 387, 1294, 1626, 1995, 2227
EcoT38I GRGCYC 2 cut(s) 1170, 1659
ErhI CCWWGG 6 cut(s) 85, 387, 1294, 1626, 1995, 2227
FalI AAGNNNNNCTT 2 cut(s) 193, 225
FaqI GGGAC 2 cut(s) 155, 1757
FauI CCCGC 2 cut(s) 760, 2098
FauNDI CATATG 1 cut(s) 2337
FbaI TGATCA 2 cut(s) 2611, 2713
FblI GTMKAC 1 cut(s) 2223
Fnu4HI GCNGC 6 cut(s) 565, 788, 888, 1339, 1349, 2423
FokI GGATG 2 cut(s) 136, 236
FriOI GRGCYC 2 cut(s) 1170, 1659
Fsp4HI GCNGC 6 cut(s) 565, 788, 888, 1339, 1349, 2423
FspBI CTAG 9 cut(s) 11, 75, 420, 1320, 1769, 2147, 2522, 2618, 2772
GlaI GCGC 4 cut(s) 724, 1163, 2393, 2421
GluI GCNGC 6 cut(s) 565, 788, 888, 1339, 1349, 2423
GsuI CTGGAG 2 cut(s) 1248, 2097
HaeII RGCGCY 2 cut(s) 726, 2395
HaeIII GGCC 2 cut(s) 104, 2591
HapII CCGG 3 cut(s) 570, 1058, 2206
HgaI GACGC 1 cut(s) 2030
HhaI GCGC 4 cut(s) 725, 1164, 2394, 2422
Hin6I GCGC 4 cut(s) 723, 1162, 2392, 2420
HinP1I GCGC 4 cut(s) 723, 1162, 2392, 2420
HincII GTYRAC 4 cut(s) 516, 2224, 2533, 2667
HindII GTYRAC 4 cut(s) 516, 2224, 2533, 2667
HpaI GTTAAC 1 cut(s) 2667
HpaII CCGG 3 cut(s) 570, 1058, 2206
HphI GGTGA 4 cut(s) 58, 101, 1694, 2011
Hpy166II GTNNAC 7 cut(s) 516, 608, 1992, 2095, 2224, 2533, 2667
Hpy8I GTNNAC 7 cut(s) 516, 608, 1992, 2095, 2224, 2533, 2667
Hpy99I CGWCG 2 cut(s) 1698, 2636
HpyAV CCTTC 9 cut(s) 37, 1123, 1199, 1457, 1803, 2446, 2504, 2776, 2842
HpyCH4III ACNGT 4 cut(s) 513, 605, 1648, 2798
HpyCH4IV ACGT 5 cut(s) 159, 655, 1676, 2097, 2374
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 570, 1061, 1226, 2180
HpyF3I CTNAG 6 cut(s) 137, 282, 930, 1916, 1985, 2652
HpySE526I ACGT 5 cut(s) 159, 655, 1676, 2097, 2374
HspAI GCGC 4 cut(s) 723, 1162, 2392, 2420
Kpn2I TCCGGA 1 cut(s) 2205
Ksp22I TGATCA 2 cut(s) 2611, 2713
KspAI GTTAAC 1 cut(s) 2667
LguI GCTCTTC 2 cut(s) 316, 480
LmnI GCTCC 5 cut(s) 572, 1060, 1654, 2642, 2841
Lsp1109I GCAGC 4 cut(s) 799, 899, 1360, 2434
LweI GCATC 3 cut(s) 114, 214, 1464
MaeI CTAG 9 cut(s) 11, 75, 420, 1320, 1769, 2147, 2522, 2618, 2772
MaeII ACGT 5 cut(s) 159, 655, 1676, 2097, 2374
MaeIII GTNAC 8 cut(s) 651, 1145, 1281, 1751, 1999, 2142, 2694, 2798
MbiI CCGCTC 1 cut(s) 767
MflI RGATCY 4 cut(s) 973, 1298, 1363, 1981
MhlI GDGCHC 3 cut(s) 1170, 1176, 1659
MlyI GAGTC 4 cut(s) 137, 1438, 1759, 2528
MmeI TCCRAC 1 cut(s) 2395
MroI TCCGGA 1 cut(s) 2205
MseI TTAA 9 cut(s) 351, 692, 1424, 1548, 1598, 1733, 1815, 2396, 2666
MslI CAYNNNNRTG 3 cut(s) 90, 584, 1997
MspA1I CMGCKG 2 cut(s) 890, 1229
MspI CCGG 3 cut(s) 570, 1058, 2206
MspR9I CCNGG 6 cut(s) 106, 561, 1014, 1111, 1479, 2352
Mva1269I GAATGC 2 cut(s) 1964, 2473
MvaI CCWGG 6 cut(s) 106, 561, 1014, 1111, 1479, 2352
MvnI CGCG 3 cut(s) 1162, 1164, 1434
MwoI GCNNNNNNNGC 5 cut(s) 11, 570, 1061, 1226, 2180
NcoI CCATGG 1 cut(s) 85
NdeI CATATG 1 cut(s) 2337
NlaIV GGNNCC 3 cut(s) 568, 1656, 2476
NmuCI GTSAC 4 cut(s) 651, 1145, 1751, 1999
NruI TCGCGA 1 cut(s) 1434
NspI RCATGY 3 cut(s) 1408, 1788, 2755
OliI CACNNNNGTG 2 cut(s) 584, 1997
PaeR7I CTCGAG 2 cut(s) 458, 1169
PagI TCATGA 1 cut(s) 817
PceI AGGCCT 2 cut(s) 104, 2591
PciSI GCTCTTC 2 cut(s) 316, 480
PctI GAATGC 2 cut(s) 1964, 2473
PfeI GAWTC 7 cut(s) 250, 289, 392, 980, 1316, 2686, 2862
PkrI GCNGC 6 cut(s) 566, 789, 889, 1340, 1350, 2424
PleI GAGTC 4 cut(s) 137, 1438, 1758, 2528
PpsI GAGTC 4 cut(s) 137, 1438, 1758, 2528
PpuMI RGGWCCY 1 cut(s) 2491
PshAI GACNNNNGTC 1 cut(s) 1749
Psp124BI GAGCTC 1 cut(s) 1170
Psp5II RGGWCCY 1 cut(s) 2491
Psp6I CCWGG 6 cut(s) 104, 559, 1012, 1109, 1477, 2350
PspGI CCWGG 6 cut(s) 104, 559, 1012, 1109, 1477, 2350
PspN4I GGNNCC 3 cut(s) 568, 1656, 2476
PspPI GGNCC 6 cut(s) 61, 196, 557, 1989, 2354, 2491
PspPPI RGGWCCY 1 cut(s) 2491
PspXI VCTCGAGB 1 cut(s) 1169
PstI CTGCAG 1 cut(s) 1667
PstNI CAGNNNCTG 1 cut(s) 2689
PsuI RGATCY 4 cut(s) 973, 1298, 1363, 1981
PvuII CAGCTG 2 cut(s) 890, 1229
RruI TCGCGA 1 cut(s) 1434
RsaI GTAC 9 cut(s) 615, 969, 1237, 1669, 1762, 1812, 1857, 1907, 2298
RsaNI GTAC 9 cut(s) 614, 968, 1236, 1668, 1761, 1811, 1856, 1906, 2297
RseI CAYNNNNRTG 3 cut(s) 90, 584, 1997
SacI GAGCTC 1 cut(s) 1170
SalI GTCGAC 1 cut(s) 2222
SapI GCTCTTC 2 cut(s) 316, 480
SaqAI TTAA 9 cut(s) 351, 692, 1424, 1548, 1598, 1733, 1815, 2396, 2666
SatI GCNGC 6 cut(s) 565, 788, 888, 1339, 1349, 2423
Sau96I GGNCC 6 cut(s) 61, 196, 557, 1989, 2354, 2491
ScaI AGTACT 1 cut(s) 1669
SchI GAGTC 4 cut(s) 137, 1438, 1759, 2528
ScrFI CCNGG 6 cut(s) 106, 561, 1014, 1111, 1479, 2352
SduI GDGCHC 3 cut(s) 1170, 1176, 1659
SfaNI GCATC 3 cut(s) 114, 214, 1464
SfcI CTRYAG 3 cut(s) 21, 1663, 2777
Sfr274I CTCGAG 2 cut(s) 458, 1169
SinI GGWCC 6 cut(s) 61, 196, 557, 1989, 2354, 2491
SlaI CTCGAG 2 cut(s) 458, 1169
SmiMI CAYNNNNRTG 3 cut(s) 90, 584, 1997
SmlI CTYRAG 7 cut(s) 404, 458, 1169, 1747, 2185, 2291, 2657
SmoI CTYRAG 7 cut(s) 404, 458, 1169, 1747, 2185, 2291, 2657
SpeI ACTAGT 2 cut(s) 74, 2146
SseBI AGGCCT 2 cut(s) 104, 2591
SsiI CCGC 8 cut(s) 564, 767, 785, 1160, 1339, 1894, 2105, 2408
SspI AATATT 3 cut(s) 30, 2113, 2815
SspMI CTAG 9 cut(s) 11, 75, 420, 1320, 1769, 2147, 2522, 2618, 2772
SstI GAGCTC 1 cut(s) 1170
StuI AGGCCT 2 cut(s) 104, 2591
StyD4I CCNGG 6 cut(s) 104, 559, 1012, 1109, 1477, 2350
StyI CCWWGG 6 cut(s) 85, 387, 1294, 1626, 1995, 2227
TaaI ACNGT 4 cut(s) 513, 605, 1648, 2798
TaiI ACGT 5 cut(s) 162, 658, 1679, 2100, 2377
TaqI TCGA 9 cut(s) 459, 696, 1170, 1576, 1716, 2223, 2325, 2634, 2818
TatI WGTACW 2 cut(s) 967, 1667
TauI GCSGC 2 cut(s) 567, 1341
TfiI GAWTC 7 cut(s) 250, 289, 392, 980, 1316, 2686, 2862
Tru1I TTAA 9 cut(s) 351, 692, 1424, 1548, 1598, 1733, 1815, 2396, 2666
Tru9I TTAA 9 cut(s) 351, 692, 1424, 1548, 1598, 1733, 1815, 2396, 2666
TscAI CASTG 5 cut(s) 1181, 1651, 2417, 2548, 2863
TseFI GTSAC 4 cut(s) 651, 1145, 1751, 1999
TseI GCWGC 4 cut(s) 787, 887, 1348, 2422
Tsp45I GTSAC 4 cut(s) 651, 1145, 1751, 1999
TspGWI ACGGA 1 cut(s) 1054
TspRI CASTG 5 cut(s) 1181, 1651, 2417, 2548, 2863
VpaK11BI GGWCC 6 cut(s) 61, 196, 557, 1989, 2354, 2491
XagI CCTNNNNNAGG 1 cut(s) 136
XapI RAATTY 8 cut(s) 526, 1689, 1826, 2027, 2341, 2400, 2606, 2762
XceI RCATGY 3 cut(s) 1408, 1788, 2755
XcmI CCANNNNNNNNNTGG 2 cut(s) 2834, 2855
XhoI CTCGAG 2 cut(s) 458, 1169
XmiI GTMKAC 1 cut(s) 2223
XspI CTAG 9 cut(s) 11, 75, 420, 1320, 1769, 2147, 2522, 2618, 2772
ZrmI AGTACT 1 cut(s) 1669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.