Rorug05G0101600

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000865
Physical Location & Seq
Forward (+)
8931974 .. 8934244
2271 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0101600.1

Sequence Viewer

Length: 954 bp
ATGCCCCTATCCCCTGTCAAGGAAGACCCTGATGGCCCGTCGTCCTTGTTTGAAGACCCTAGCCAGGACCAGAACCTAACCCTAACCCCTGTCACGGAAGACCCTGTTGTCCACGACCCGGACCCAGCCATGCCCCTATCCCCTATCGTGGTCCGCCTGAAGCGGTACCGTTCTTCCTTGTTTGAAGACCCTAGCAAGGTCCAGAACCTAACCCTAACCCCTGTCACGGAAGACCCTGATGTTGTGAAGCGGCCCCGTTCGTCTTTGTCCTCTGAAGACCCTGATGTCCAGGGAGGCAAGCACCAGGACCCGGACCCGGTCCAGCCCGCGAAGCCCTTAAAACCAGTAAGCCGTACGCTGAAGGAGATTCTCGATGAGTCCGGCCCGTACATGTCTAAAGAGCAAGAGCTCGAGTATGCTATGCTTTTTGCTAAGTGGAAGGAGGCCGAACCTATTACCAATCGTGTGATGGCCTCCATTCCGGAGCCTCTTTTTGGTGGACGCATTAACGTAGTACAGAGTTATGGTCCTGATACGCTATCCGTAGATATGGCAACAATTGATGGATACTGTCAGAGTCTGGCGATGTTGTCAATGGAGTCTCTGAGACGTAGAAGGGCAGAAAACCCAAAGTATTGTGGAGAAGCAGCATTGAACGAATACATCGAACGTCAAAAGAGTACATGGGGGCCGTGTGAGATTTGTTTTAAGGCTGGTGACCATATTAGTTTGTACTGCCCATATCGTGACTATGTCCCGGAAGGCGCATCTGTTGGCCCTGGCGTTGAACTAGTTTGTAGGAATTGTGGACTTGCGGACCAGCACCCTGGTGCTAAACGTGCTATTCTCAAGTCGTGTTTCATTTGTGCTTCGGACCACTGGGGTACCGATTGCCCGCGTGCTAAACAGCATAAAACGGTTCCATATAATCCCTTTCCCTTCAGCGACCGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04626 map04626
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

317

Amino Acids

35.29

Weight (kDa)

5.2

Isoelectric Point (pI)

58.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 107, 284
Acc65I GGTACC 2 cut(s) 165, 884
AccB1I GGYRCC 2 cut(s) 165, 884
AccII CGCG 2 cut(s) 329, 898
AccIII TCCGGA 1 cut(s) 481
AciI CCGC 7 cut(s) 154, 163, 250, 327, 815, 896, 949
AcuI CTGAAG 4 cut(s) 179, 294, 380, 925
AfaI GTAC 7 cut(s) 167, 355, 389, 516, 682, 734, 886
AflIII ACRYGT 1 cut(s) 390
AgsI TTSAA 4 cut(s) 53, 185, 655, 788
AhlI ACTAGT 1 cut(s) 790
AjnI CCWGG 5 cut(s) 63, 288, 303, 778, 826
AleI CACNNNNGTG 1 cut(s) 828
AluBI AGCT 1 cut(s) 409
AluI AGCT 1 cut(s) 409
Alw21I GWGCWC 1 cut(s) 411
Alw26I GTCTC 2 cut(s) 601, 606
AlwNI CAGNNNCTG 1 cut(s) 580
Ama87I CYCGRG 1 cut(s) 410
Aor13HI TCCGGA 1 cut(s) 481
AoxI GGCC 7 cut(s) 34, 251, 382, 444, 471, 689, 775
ApeKI GCWGC 1 cut(s) 647
Asp718I GGTACC 2 cut(s) 165, 884
AspLEI GCGC 1 cut(s) 767
AsuC2I CCSGG 4 cut(s) 119, 311, 317, 758
AsuHPI GGTGA 1 cut(s) 728
AvaI CYCGRG 1 cut(s) 410
BaeI ACNNNNGTAYC 2 cut(s) 868, 901
BanI GGYRCC 2 cut(s) 165, 884
BanII GRGCYC 1 cut(s) 411
BbsI GAAGAC 6 cut(s) 30, 60, 105, 192, 237, 282
Bbv12I GWGCWC 1 cut(s) 411
BbvI GCAGC 1 cut(s) 659
BccI CCATC 3 cut(s) 26, 463, 557
BceAI ACGGC 2 cut(s) 336, 676
BciT130I CCWGG 5 cut(s) 65, 290, 305, 780, 828
BciVI GTATCC 1 cut(s) 560
BcnI CCSGG 4 cut(s) 119, 311, 317, 758
BcoDI GTCTC 2 cut(s) 601, 606
BcuI ACTAGT 1 cut(s) 790
BfaI CTAG 3 cut(s) 60, 192, 791
BfuI GTATCC 1 cut(s) 560
BisI GCNGC 2 cut(s) 251, 648
BlsI GCNGC 2 cut(s) 252, 649
Bme1390I CCNGG 9 cut(s) 65, 119, 290, 305, 311, 317, 758, 780, 828
BmeT110I CYCGRG 1 cut(s) 410
BmiI GGNNCC 9 cut(s) 123, 167, 254, 309, 315, 486, 690, 886, 921
BmrFI CCNGG 9 cut(s) 65, 119, 290, 305, 311, 317, 758, 780, 828
BmrI ACTGGG 1 cut(s) 889
BmsI GCATC 1 cut(s) 776
BmuI ACTGGG 1 cut(s) 889
BpiI GAAGAC 6 cut(s) 30, 60, 105, 192, 237, 282
BpuEI CTTGAG 1 cut(s) 833
BpuMI CCSGG 4 cut(s) 119, 311, 317, 758
BsaJI CCNNGG 3 cut(s) 289, 778, 826
BsaWI WCCGGW 1 cut(s) 481
Bse1I ACTGG 2 cut(s) 344, 884
BseAI TCCGGA 1 cut(s) 481
BseBI CCWGG 5 cut(s) 65, 290, 305, 780, 828
BseDI CCNNGG 3 cut(s) 289, 778, 826
BseMII CTCAG 1 cut(s) 596
BseNI ACTGG 2 cut(s) 344, 884
BseXI GCAGC 1 cut(s) 659
BseYI CCCAGC 1 cut(s) 124
Bsh1236I CGCG 2 cut(s) 329, 898
Bsh1285I CGRYCG 1 cut(s) 949
BshFI GGCC 7 cut(s) 36, 253, 384, 446, 473, 691, 777
BshNI GGYRCC 2 cut(s) 165, 884
BsiEI CGRYCG 1 cut(s) 949
BsiHKAI GWGCWC 1 cut(s) 411
BsiHKCI CYCGRG 1 cut(s) 410
BsiSI CCGG 6 cut(s) 119, 311, 317, 381, 482, 758
BsiWI CGTACG 1 cut(s) 353
BslFI GGGAC 1 cut(s) 740
BsmAI GTCTC 2 cut(s) 601, 606
BsmBI CGTCTC 1 cut(s) 601
BsmFI GGGAC 1 cut(s) 740
BsnI GGCC 7 cut(s) 36, 253, 384, 446, 473, 691, 777
BsoBI CYCGRG 1 cut(s) 410
Bsp1286I GDGCHC 1 cut(s) 411
Bsp13I TCCGGA 1 cut(s) 481
BspACI CCGC 7 cut(s) 154, 163, 250, 327, 815, 896, 949
BspANI GGCC 7 cut(s) 36, 253, 384, 446, 473, 691, 777
BspCNI CTCAG 1 cut(s) 597
BspEI TCCGGA 1 cut(s) 481
BspFNI CGCG 2 cut(s) 329, 898
BspLI GGNNCC 9 cut(s) 123, 167, 254, 309, 315, 486, 690, 886, 921
BspT107I GGYRCC 2 cut(s) 165, 884
BsrI ACTGG 2 cut(s) 344, 884
BssECI CCNNGG 3 cut(s) 289, 778, 826
Bst2UI CCWGG 5 cut(s) 65, 290, 305, 780, 828
Bst4CI ACNGT 3 cut(s) 170, 572, 919
BstC8I GCNNGC 4 cut(s) 299, 327, 896, 900
BstDEI CTNAG 2 cut(s) 432, 605
BstEII GGTNACC 1 cut(s) 716
BstFNI CGCG 2 cut(s) 329, 898
BstHHI GCGC 1 cut(s) 767
BstMAI GTCTC 2 cut(s) 601, 606
BstMCI CGRYCG 1 cut(s) 949
BstMWI GCNNNNNNNGC 2 cut(s) 331, 839
BstNI CCWGG 5 cut(s) 65, 290, 305, 780, 828
BstNSI RCATGY 1 cut(s) 394
BstPI GGTNACC 1 cut(s) 716
BstSCI CCNGG 9 cut(s) 63, 117, 288, 303, 309, 315, 756, 778, 826
BstUI CGCG 2 cut(s) 329, 898
BstV1I GCAGC 1 cut(s) 659
BstV2I GAAGAC 6 cut(s) 30, 60, 105, 192, 237, 282
BstXI CCANNNNNNTGG 1 cut(s) 827
BsuI GTATCC 1 cut(s) 560
BsuRI GGCC 7 cut(s) 36, 253, 384, 446, 473, 691, 777
BtgZI GCGATG 1 cut(s) 599
BtsIMutI CAGTG 1 cut(s) 877
Cac8I GCNNGC 4 cut(s) 299, 327, 896, 900
CaiI CAGNNNCTG 1 cut(s) 580
CfoI GCGC 1 cut(s) 767
CseI GACGC 1 cut(s) 510
Csp6I GTAC 7 cut(s) 166, 354, 388, 515, 681, 733, 885
CviAII CATG 3 cut(s) 130, 391, 684
CviQI GTAC 7 cut(s) 166, 354, 388, 515, 681, 733, 885
DdeI CTNAG 2 cut(s) 432, 605
DrdI GACNNNNNNGTC 2 cut(s) 107, 284
DseDI GACNNNNNNGTC 2 cut(s) 107, 284
EciI GGCGGA 1 cut(s) 143
Ecl136II GAGCTC 1 cut(s) 409
Eco24I GRGCYC 1 cut(s) 411
Eco53kI GAGCTC 1 cut(s) 409
Eco57I CTGAAG 4 cut(s) 179, 294, 380, 925
Eco88I CYCGRG 1 cut(s) 410
Eco91I GGTNACC 1 cut(s) 716
EcoICRI GAGCTC 1 cut(s) 409
EcoO109I RGGNCCY 1 cut(s) 307
EcoO65I GGTNACC 1 cut(s) 716
EcoRII CCWGG 5 cut(s) 63, 288, 303, 778, 826
EcoT38I GRGCYC 1 cut(s) 411
Esp3I CGTCTC 1 cut(s) 601
FaeI CATG 3 cut(s) 133, 394, 687
FaqI GGGAC 1 cut(s) 740
FatI CATG 3 cut(s) 129, 390, 683
FauI CCCGC 2 cut(s) 334, 903
Fnu4HI GCNGC 2 cut(s) 251, 648
FriOI GRGCYC 1 cut(s) 411
Fsp4HI GCNGC 2 cut(s) 251, 648
FspBI CTAG 3 cut(s) 60, 192, 791
GlaI GCGC 1 cut(s) 766
GluI GCNGC 2 cut(s) 251, 648
GsaI CCCAGC 1 cut(s) 128
HaeIII GGCC 7 cut(s) 36, 253, 384, 446, 473, 691, 777
HapII CCGG 6 cut(s) 119, 311, 317, 381, 482, 758
HgaI GACGC 1 cut(s) 510
HhaI GCGC 1 cut(s) 767
Hin1II CATG 3 cut(s) 133, 394, 687
Hin6I GCGC 1 cut(s) 765
HinP1I GCGC 1 cut(s) 765
HinfI GANTC 4 cut(s) 367, 377, 577, 599
HpaII CCGG 6 cut(s) 119, 311, 317, 381, 482, 758
HphI GGTGA 1 cut(s) 728
Hpy166II GTNNAC 3 cut(s) 112, 500, 809
Hpy188I TCNGA 4 cut(s) 274, 576, 606, 874
Hpy188III TCNNGA 5 cut(s) 202, 371, 482, 530, 746
Hpy8I GTNNAC 3 cut(s) 112, 500, 809
Hpy99I CGWCG 1 cut(s) 43
HpyAV CCTTC 5 cut(s) 355, 433, 609, 755, 949
HpyCH4III ACNGT 3 cut(s) 170, 572, 919
HpyCH4IV ACGT 4 cut(s) 510, 610, 670, 838
HpyF10VI GCNNNNNNNGC 2 cut(s) 331, 839
HpyF3I CTNAG 2 cut(s) 432, 605
HpySE526I ACGT 4 cut(s) 510, 610, 670, 838
Hsp92II CATG 3 cut(s) 133, 394, 687
HspAI GCGC 1 cut(s) 765
Kpn2I TCCGGA 1 cut(s) 481
KpnI GGTACC 2 cut(s) 169, 888
LmnI GCTCC 1 cut(s) 484
Lsp1109I GCAGC 1 cut(s) 659
LweI GCATC 1 cut(s) 776
MaeI CTAG 3 cut(s) 60, 192, 791
MaeII ACGT 4 cut(s) 510, 610, 670, 838
MaeIII GTNAC 4 cut(s) 91, 223, 716, 746
MboII GAAGA 7 cut(s) 35, 65, 110, 165, 197, 242, 287
MfeI CAATTG 1 cut(s) 558
MhlI GDGCHC 1 cut(s) 411
MluCI AATT 2 cut(s) 558, 802
MlyI GAGTC 3 cut(s) 386, 586, 608
MnlI CCTC 5 cut(s) 280, 287, 436, 484, 498
MroI TCCGGA 1 cut(s) 481
MseI TTAA 3 cut(s) 338, 507, 708
MslI CAYNNNNRTG 1 cut(s) 828
MspI CCGG 6 cut(s) 119, 311, 317, 381, 482, 758
MspR9I CCNGG 9 cut(s) 65, 119, 290, 305, 311, 317, 758, 780, 828
MunI CAATTG 1 cut(s) 558
MvaI CCWGG 5 cut(s) 65, 290, 305, 780, 828
MvnI CGCG 2 cut(s) 329, 898
MwoI GCNNNNNNNGC 2 cut(s) 331, 839
NciI CCSGG 4 cut(s) 119, 311, 317, 758
NlaIII CATG 3 cut(s) 133, 394, 687
NlaIV GGNNCC 9 cut(s) 123, 167, 254, 309, 315, 486, 690, 886, 921
NmuCI GTSAC 4 cut(s) 91, 223, 716, 746
NspI RCATGY 1 cut(s) 394
OliI CACNNNNGTG 1 cut(s) 828
PaeR7I CTCGAG 1 cut(s) 410
PciI ACATGT 1 cut(s) 390
PcsI WCGNNNNNNNCGW 1 cut(s) 663
PfeI GAWTC 1 cut(s) 367
Pfl23II CGTACG 1 cut(s) 353
PflFI GACNNNGTC 2 cut(s) 317, 752
PfoI TCCNGGA 1 cut(s) 756
PkrI GCNGC 2 cut(s) 252, 649
PleI GAGTC 3 cut(s) 385, 585, 607
PpsI GAGTC 3 cut(s) 385, 585, 607
PpuMI RGGWCCY 1 cut(s) 307
PscI ACATGT 1 cut(s) 390
Psp124BI GAGCTC 1 cut(s) 411
Psp5II RGGWCCY 1 cut(s) 307
Psp6I CCWGG 5 cut(s) 63, 288, 303, 778, 826
PspEI GGTNACC 1 cut(s) 716
PspFI CCCAGC 1 cut(s) 124
PspGI CCWGG 5 cut(s) 63, 288, 303, 778, 826
PspLI CGTACG 1 cut(s) 353
PspN4I GGNNCC 9 cut(s) 123, 167, 254, 309, 315, 486, 690, 886, 921
PspPPI RGGWCCY 1 cut(s) 307
PspXI VCTCGAGB 1 cut(s) 410
PstNI CAGNNNCTG 1 cut(s) 580
PsyI GACNNNGTC 2 cut(s) 317, 752
RsaI GTAC 7 cut(s) 167, 355, 389, 516, 682, 734, 886
RsaNI GTAC 7 cut(s) 166, 354, 388, 515, 681, 733, 885
RseI CAYNNNNRTG 1 cut(s) 828
SacI GAGCTC 1 cut(s) 411
SaqAI TTAA 3 cut(s) 338, 507, 708
SatI GCNGC 2 cut(s) 251, 648
SchI GAGTC 3 cut(s) 386, 586, 608
ScrFI CCNGG 9 cut(s) 65, 119, 290, 305, 311, 317, 758, 780, 828
SduI GDGCHC 1 cut(s) 411
SetI ASST 9 cut(s) 78, 201, 210, 411, 454, 513, 613, 673, 841
SfaNI GCATC 1 cut(s) 776
Sfr274I CTCGAG 1 cut(s) 410
SlaI CTCGAG 1 cut(s) 410
SmiMI CAYNNNNRTG 1 cut(s) 828
SmlI CTYRAG 2 cut(s) 410, 848
SmoI CTYRAG 2 cut(s) 410, 848
SpeI ACTAGT 1 cut(s) 790
Sse9I AATT 2 cut(s) 558, 802
SsiI CCGC 7 cut(s) 154, 163, 250, 327, 815, 896, 949
SspMI CTAG 3 cut(s) 60, 192, 791
SstI GAGCTC 1 cut(s) 411
StyD4I CCNGG 9 cut(s) 63, 117, 288, 303, 309, 315, 756, 778, 826
TaaI ACNGT 3 cut(s) 170, 572, 919
TaiI ACGT 4 cut(s) 513, 613, 673, 841
TaqI TCGA 3 cut(s) 372, 411, 666
TasI AATT 2 cut(s) 558, 802
TatI WGTACW 3 cut(s) 514, 680, 732
TauI GCSGC 1 cut(s) 253
TfiI GAWTC 1 cut(s) 367
Tru1I TTAA 3 cut(s) 338, 507, 708
Tru9I TTAA 3 cut(s) 338, 507, 708
TscAI CASTG 1 cut(s) 884
TseFI GTSAC 4 cut(s) 91, 223, 716, 746
TseI GCWGC 1 cut(s) 647
Tsp45I GTSAC 4 cut(s) 91, 223, 716, 746
TspDTI ATGAA 1 cut(s) 850
TspGWI ACGGA 3 cut(s) 110, 242, 532
TspRI CASTG 1 cut(s) 884
Tth111I GACNNNGTC 2 cut(s) 317, 752
XceI RCATGY 1 cut(s) 394
XcmI CCANNNNNNNNNTGG 1 cut(s) 466
XhoI CTCGAG 1 cut(s) 410
XspI CTAG 3 cut(s) 60, 192, 791
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.