MD05G1219900.v1.1

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000484
Physical Location & Seq
Reverse (-)
35278288 .. 35281559
3272 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1219900.v1.1.491

Sequence Viewer

Length: 2196 bp
ATGAATTTAATGAAAAAACCATTGAAAGCTTCAACTAAGATGTGCTTGCTTCTCTTCTACTCTTCTTTGCTGCTCATCTTCGCAGCAGTATTCTCCATAGCTGATGACGGTGAGACTGTAGTTTCAACTGGGGGAACATTTGAGCTAGGATTTTACAGTCCTGATGCTTCTAATAGACGGTACGTGGGGATATGGTACAAGAAGATTTCTGTTAAAACCATTGTATGGGTTGCCAACAGAGACACACCCCTCACTGATTTGTCTGGCGTTCTGAAGGTCACCAACCCCGGAATACTTGTGCTCAACCACAACATGAGCACAATTTGGTCCTCCAACACATCGAGAACTGCACAGAATCCAATGGCACGTCTTTTGGATTCGGGTAATCTTATCGTGATAGATATGAGTGATGATGACTCTGAGAACCTTCTGTGGCAAAGTTTTGATTACCCTGGCGATACATTCTTACCAGGTATGAAGCTTGGTAGGAACACAGTTACAGGCTTCAATTGGCATCTTAGATCGTCGAAAAGTCCTCAGGATCCTTCTCAAGGAAATCGTACATCTCAACTTGGTCCCAAAGGATATGCAGAATTATTTGTGAGGGAAGGTTCGGTCATTAAATATCGGACTGGACCATGGAATGGAGTAGGGTTCAGTGGAAGCCCTCAGTTCAATCCAAACCCTATATTCACATACATTTTTGTTTCTGAGCCTGATGAAATGTACTACAGTTACAAGCTTCACAACAGCTCAATCTTTTCGAGGGTGGTGTTAACTTCAGATGGACTTGTGCAGCGCGCTACACATGGATTGATAGAACCAAAGTCCCCGGTTTGTAGTTGTTTGAAAGGATTTACACCAAAGTTCCCGAAAGAATGGGATTTGGTGGATTGGTCTCATGGCTGTGTGAGAAAGACTTCTCTAAATTGCACTGGAGATGTGTTCCAAAAGTACTCGGGGGTGAAATTGCCTAGCACAGAACAATCATGGCATAACAAAAGTATGAACCTCAAGGAATGTGAGATGCTGTGCATGAAGAACTGCTCCTGCACGGCTTATACAAATTTGGATATCCGGGATGGAGGAACTGGGTGCTTGCTGTGGTACGGTGATCTAATTGATATAAGATACACTGCTGAAAACGGGCAAGATATTTATATAAGAATGGCCGCAGCAGAACAAGGAAAATTGGGACGCAGCCAAAATGAGGATCTGGAATTACCGTTATTTGACTTGATGACTATAGTTTCTGCAACCAATAACTTTTCAATTGAAAACAAACTTGGTGAAGGCGGTTTCGGATCTGTCTTTAAGGGTACGATGCAAGATGGACAAGAAATCGCAGTGAAAAGGCTTTCAAAAAATTCTAGACAAGGACTCAACGAGTTCAAGAATGAAGTTACACATATTGCCAAACTTCAGCACAGGAATCTAGTAAAACTTCTTGGATGTTGCATTCAAGAAGACGAGATGATACTGATCTACGAGTTCATGCCTAACAAGAGCTTAGACTTCTTTATTTTTGACTGGGCGAAGCGCTTTGAAATTATTAATGGGATAGCTCGAGGGCTCCTCTATCTTCATCAAGATTCTAGATTGAGAGTTATTCATAGAGATCTCAAAGCAAGTAACATTTTATTAGGCAGTGAATTTAACCCGAAAATCTCAGACTTTGGCCTGGCTAGAAGCTTTGGAGGAATTGAAACGAAAGCAGAAACGAAGAAAGTGGTCGGAACATACGGTTACATGTCCCCAGAATATGCAATTGATGGTTTCTACTCTATAAAGTCCGACGTCTATAGCTTTGGTGTTCTGGTGCTAGAGATAGTGAGTGGGAGTAGAAATAGAGGATTCTCTCATCCAGACCACAAACTCAACCTTCTTGGACATGCATGGATGCTACACACAGAAGGCAGGCCTCTCGAACTGCTTGATGCATCGATAGAGGACTCCGTCACTCTGCATGAAGTTGTGAGAATGATTCATGTGGGTCTTTTGTGTGTGCAGAGGAATCCAGAAGACAAGCCGAGCATGTCAGCCGCAGTTCTAATGTTGGGTAGTGAAGGTGCATTGCCCCCACCTCTAAAACCTGGTTTTTACAGTGAAAGGGATATGAGTGAACTCGAAGTCGGTCATTCTTCTAAAGCATGCTCAGCTAATGAAGTCACTATTTCACTAGTCGAGGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase_Tyr S_locus_glycop

Protein Analysis

732

Amino Acids

81.62

Weight (kDa)

6.52

Isoelectric Point (pI)

43.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 73 - 177 9.8e-36 D-mannose binding lectin
S_locus_glycop PF00954 209 - 269 3.5e-09 S-locus glycoprotein domain
PAN_2 PF08276 311 - 376 1.1e-19 PAN-like domain
PK_Tyr_Ser-Thr PF07714 424 - 616 4.4e-44 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 424 - 615 8.5e-43 Protein kinase domain
DUF3403 PF11883 689 - 731 3.7e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1800
AccB7I CCANNNNNTGG 2 cut(s) 225, 644
AccII CGCG 1 cut(s) 801
AciI CCGC 3 cut(s) 1173, 1296, 2043
AclWI GGATC 4 cut(s) 536, 549, 1221, 1312
AcoI YGGCCR 1 cut(s) 1170
AcsI RAATTY 4 cut(s) 4, 1066, 1366, 1652
AcuI CTGAAG 3 cut(s) 293, 765, 1406
AcyI GRCGYC 1 cut(s) 1797
AfaI GTAC 7 cut(s) 182, 197, 562, 728, 956, 1109, 1321
AfeI AGCGCT 1 cut(s) 1541
AfiI CCNNNNNNNGG 4 cut(s) 225, 551, 644, 1210
AflIII ACRYGT 1 cut(s) 1749
AhlI ACTAGT 1 cut(s) 2179
AjiI CACGTC 1 cut(s) 368
AjnI CCWGG 4 cut(s) 451, 469, 1680, 2092
AjuI GAANNNNNNNTTGG 2 cut(s) 1269, 1301
Alw21I GWGCWC 2 cut(s) 303, 320
Alw26I GTCTC 3 cut(s) 107, 234, 903
AlwI GGATC 4 cut(s) 536, 549, 1221, 1312
Ama87I CYCGRG 2 cut(s) 958, 1566
Aor51HI AGCGCT 1 cut(s) 1541
AoxI GGCC 3 cut(s) 1170, 1678, 1919
ApeKI GCWGC 5 cut(s) 70, 83, 796, 1175, 1200
ApoI RAATTY 4 cut(s) 4, 1066, 1366, 1652
AseI ATTAAT 1 cut(s) 1554
Asp700I GAANNNNTTC 1 cut(s) 919
AspLEI GCGC 3 cut(s) 801, 803, 1542
AspS9I GGNCC 3 cut(s) 327, 575, 635
AsuC2I CCSGG 3 cut(s) 288, 833, 1079
AsuHPI GGTGA 5 cut(s) 122, 271, 976, 1124, 1301
AvaI CYCGRG 2 cut(s) 958, 1566
AvaII GGWCC 3 cut(s) 327, 575, 635
AxyI CCTNAGG 1 cut(s) 537
BamHI GGATCC 1 cut(s) 541
BanII GRGCYC 1 cut(s) 1575
BbsI GAAGAC 2 cut(s) 1473, 2028
Bbv12I GWGCWC 2 cut(s) 303, 320
BbvI GCAGC 5 cut(s) 57, 95, 808, 1187, 1212
BccI CCATC 4 cut(s) 779, 1076, 1325, 1766
BceAI ACGGC 1 cut(s) 1071
BcgI CGANNNNNNTGC 2 cut(s) 1906, 1940
BciT130I CCWGG 4 cut(s) 453, 471, 1682, 2094
BcnI CCSGG 3 cut(s) 288, 833, 1079
BcoDI GTCTC 3 cut(s) 107, 234, 903
BcuI ACTAGT 1 cut(s) 2179
BfaI CTAG 8 cut(s) 146, 975, 1371, 1436, 1596, 1686, 1823, 2180
BfmI CTRYAG 4 cut(s) 117, 730, 1245, 1801
BfoI RGCGCY 1 cut(s) 1543
BglII AGATCT 1 cut(s) 1618
BisI GCNGC 7 cut(s) 71, 84, 797, 1173, 1176, 1201, 2043
BlpI GCTNAGC 1 cut(s) 2155
BlsI GCNGC 7 cut(s) 72, 85, 798, 1174, 1177, 1202, 2044
BmcAI AGTACT 1 cut(s) 956
Bme1390I CCNGG 7 cut(s) 288, 453, 471, 833, 1079, 1682, 2094
Bme18I GGWCC 3 cut(s) 327, 575, 635
BmeT110I CYCGRG 2 cut(s) 958, 1566
BmgBI CACGTC 1 cut(s) 368
BmgT120I GGNCC 3 cut(s) 327, 575, 635
BmiI GGNNCC 3 cut(s) 543, 577, 1574
BmrFI CCNGG 7 cut(s) 288, 453, 471, 833, 1079, 1682, 2094
BmrI ACTGGG 3 cut(s) 138, 1101, 1540
BmsI GCATC 7 cut(s) 154, 523, 1017, 1314, 1890, 1927, 1949
BmuI ACTGGG 3 cut(s) 138, 1101, 1540
BpiI GAAGAC 2 cut(s) 1473, 2028
BplI GAGNNNNNCTC 2 cut(s) 1560, 1592
BpmI CTGGAG 1 cut(s) 957
Bpu1102I GCTNAGC 1 cut(s) 2155
BpuEI CTTGAG 2 cut(s) 534, 998
BpuMI CCSGG 3 cut(s) 288, 833, 1079
Bsa29I ATCGAT 1 cut(s) 1943
BsaAI YACGTR 1 cut(s) 184
BsaBI GATNNNNATC 1 cut(s) 1481
BsaHI GRCGYC 1 cut(s) 1797
BsaI GGTCTC 1 cut(s) 903
BsaJI CCNNGG 4 cut(s) 286, 451, 638, 831
Bsc4I CCNNNNNNNGG 4 cut(s) 225, 551, 644, 1210
Bse1I ACTGG 5 cut(s) 133, 637, 940, 1096, 1535
Bse21I CCTNAGG 1 cut(s) 537
Bse3DI GCAATG 1 cut(s) 2072
Bse8I GATNNNNATC 1 cut(s) 1481
BseBI CCWGG 4 cut(s) 453, 471, 1682, 2094
BseCI ATCGAT 1 cut(s) 1943
BseDI CCNNGG 4 cut(s) 286, 451, 638, 831
BseGI GGATG 4 cut(s) 1087, 1457, 1861, 1905
BseJI GATNNNNATC 1 cut(s) 1481
BseLI CCNNNNNNNGG 4 cut(s) 225, 551, 644, 1210
BseMI GCAATG 1 cut(s) 2072
BseMII CTCAG 6 cut(s) 411, 551, 683, 702, 1683, 2169
BseNI ACTGG 5 cut(s) 133, 637, 940, 1096, 1535
BsePI GCGCGC 1 cut(s) 799
BseRI GAGGAG 1 cut(s) 1565
BseXI GCAGC 5 cut(s) 57, 95, 808, 1187, 1212
BsgI GTGCAG 4 cut(s) 333, 815, 1036, 2027
Bsh1236I CGCG 1 cut(s) 801
BshFI GGCC 3 cut(s) 1172, 1680, 1921
BshVI ATCGAT 1 cut(s) 1943
BsiHKAI GWGCWC 2 cut(s) 303, 320
BsiHKCI CYCGRG 2 cut(s) 958, 1566
BsiSI CCGG 3 cut(s) 288, 833, 1078
BslFI GGGAC 4 cut(s) 561, 814, 1209, 1738
BslI CCNNNNNNNGG 4 cut(s) 225, 551, 644, 1210
BsmAI GTCTC 3 cut(s) 107, 234, 903
BsmFI GGGAC 4 cut(s) 561, 814, 1209, 1738
BsmI GAATGC 1 cut(s) 1458
BsnI GGCC 3 cut(s) 1172, 1680, 1921
Bso31I GGTCTC 1 cut(s) 903
BsoBI CYCGRG 2 cut(s) 958, 1566
Bsp1286I GDGCHC 3 cut(s) 303, 320, 1575
Bsp143I GATC 7 cut(s) 521, 541, 1114, 1213, 1304, 1482, 1618
Bsp1720I GCTNAGC 1 cut(s) 2155
Bsp19I CCATGG 1 cut(s) 638
BspACI CCGC 3 cut(s) 1173, 1296, 2043
BspANI GGCC 3 cut(s) 1172, 1680, 1921
BspCNI CTCAG 6 cut(s) 412, 550, 682, 703, 1682, 2168
BspDI ATCGAT 1 cut(s) 1943
BspFNI CGCG 1 cut(s) 801
BspLI GGNNCC 3 cut(s) 543, 577, 1574
BspPI GGATC 4 cut(s) 536, 549, 1221, 1312
BspTNI GGTCTC 1 cut(s) 903
BsrDI GCAATG 1 cut(s) 2072
BsrI ACTGG 5 cut(s) 133, 637, 940, 1096, 1535
BssECI CCNNGG 4 cut(s) 286, 451, 638, 831
BssHII GCGCGC 1 cut(s) 799
BssMI GATC 7 cut(s) 521, 541, 1114, 1213, 1304, 1482, 1618
BssNI GRCGYC 1 cut(s) 1797
BssT1I CCWWGG 1 cut(s) 638
Bst2UI CCWGG 4 cut(s) 453, 471, 1682, 2094
Bst6I CTCTTC 2 cut(s) 59, 67
BstACI GRCGYC 1 cut(s) 1797
BstBAI YACGTR 1 cut(s) 184
BstC8I GCNNGC 5 cut(s) 47, 801, 1100, 1919, 2152
BstDEI CTNAG 9 cut(s) 36, 420, 518, 537, 669, 711, 1510, 1669, 2155
BstDSI CCRYGG 1 cut(s) 638
BstEII GGTNACC 1 cut(s) 277
BstENI CCTNNNNNAGG 1 cut(s) 549
BstF5I GGATG 4 cut(s) 1087, 1457, 1861, 1905
BstFNI CGCG 1 cut(s) 801
BstH2I RGCGCY 1 cut(s) 1543
BstHHI GCGC 3 cut(s) 801, 803, 1542
BstKTI GATC 7 cut(s) 524, 544, 1117, 1216, 1307, 1485, 1621
BstMAI GTCTC 3 cut(s) 107, 234, 903
BstMBI GATC 7 cut(s) 521, 541, 1114, 1213, 1304, 1482, 1618
BstMWI GCNNNNNNNGC 1 cut(s) 2156
BstNI CCWGG 4 cut(s) 453, 471, 1682, 2094
BstNSI RCATGY 4 cut(s) 1753, 1895, 2038, 2154
BstPI GGTNACC 1 cut(s) 277
BstSCI CCNGG 7 cut(s) 286, 451, 469, 831, 1077, 1680, 2092
BstSFI CTRYAG 4 cut(s) 117, 730, 1245, 1801
BstUI CGCG 1 cut(s) 801
BstV1I GCAGC 5 cut(s) 57, 95, 808, 1187, 1212
BstV2I GAAGAC 2 cut(s) 1473, 2028
BstX2I RGATCY 4 cut(s) 541, 1213, 1304, 1618
BstYI RGATCY 4 cut(s) 541, 1213, 1304, 1618
Bsu15I ATCGAT 1 cut(s) 1943
Bsu36I CCTNAGG 1 cut(s) 537
BsuRI GGCC 3 cut(s) 1172, 1680, 1921
BsuTUI ATCGAT 1 cut(s) 1943
BtgI CCRYGG 1 cut(s) 638
BtrI CACGTC 1 cut(s) 368
BtsCI GGATG 4 cut(s) 1087, 1457, 1861, 1905
BtsI GCAGTG 3 cut(s) 1134, 1353, 1654
BtsIMutI CAGTG 7 cut(s) 252, 664, 933, 1134, 1353, 1654, 2110
Cac8I GCNNGC 5 cut(s) 47, 801, 1100, 1919, 2152
CfoI GCGC 3 cut(s) 801, 803, 1542
Cfr13I GGNCC 3 cut(s) 327, 575, 635
ClaI ATCGAT 1 cut(s) 1943
CseI GACGC 1 cut(s) 1206
CsiI ACCWGGT 2 cut(s) 469, 2092
Csp6I GTAC 7 cut(s) 181, 196, 561, 727, 955, 1108, 1320
CviQI GTAC 7 cut(s) 181, 196, 561, 727, 955, 1108, 1320
DdeI CTNAG 9 cut(s) 36, 420, 518, 537, 669, 711, 1510, 1669, 2155
DpnI GATC 7 cut(s) 523, 543, 1116, 1215, 1306, 1484, 1620
DpnII GATC 7 cut(s) 521, 541, 1114, 1213, 1304, 1482, 1618
EaeI YGGCCR 1 cut(s) 1170
Eam1104I CTCTTC 2 cut(s) 59, 67
EarI CTCTTC 2 cut(s) 59, 67
Eco130I CCWWGG 1 cut(s) 638
Eco147I AGGCCT 1 cut(s) 1921
Eco24I GRGCYC 1 cut(s) 1575
Eco31I GGTCTC 1 cut(s) 903
Eco32I GATATC 1 cut(s) 1075
Eco47I GGWCC 3 cut(s) 327, 575, 635
Eco47III AGCGCT 1 cut(s) 1541
Eco57I CTGAAG 3 cut(s) 293, 765, 1406
Eco81I CCTNAGG 1 cut(s) 537
Eco88I CYCGRG 2 cut(s) 958, 1566
Eco91I GGTNACC 1 cut(s) 277
EcoNI CCTNNNNNAGG 1 cut(s) 549
EcoO65I GGTNACC 1 cut(s) 277
EcoRII CCWGG 4 cut(s) 451, 469, 1680, 2092
EcoRV GATATC 1 cut(s) 1075
EcoT14I CCWWGG 1 cut(s) 638
EcoT22I ATGCAT 2 cut(s) 1897, 1942
EcoT38I GRGCYC 1 cut(s) 1575
ErhI CCWWGG 1 cut(s) 638
FalI AAGNNNNNCTT 2 cut(s) 29, 61
FaqI GGGAC 4 cut(s) 561, 814, 1209, 1738
Fnu4HI GCNGC 7 cut(s) 71, 84, 797, 1173, 1176, 1201, 2043
FokI GGATG 4 cut(s) 1094, 1464, 1848, 1912
FriOI GRGCYC 1 cut(s) 1575
Fsp4HI GCNGC 7 cut(s) 71, 84, 797, 1173, 1176, 1201, 2043
FspBI CTAG 8 cut(s) 146, 975, 1371, 1436, 1596, 1686, 1823, 2180
GlaI GCGC 3 cut(s) 800, 802, 1541
GluI GCNGC 7 cut(s) 71, 84, 797, 1173, 1176, 1201, 2043
GsuI CTGGAG 1 cut(s) 957
HaeII RGCGCY 1 cut(s) 1543
HaeIII GGCC 3 cut(s) 1172, 1680, 1921
HapII CCGG 3 cut(s) 288, 833, 1078
HgaI GACGC 1 cut(s) 1206
HhaI GCGC 3 cut(s) 801, 803, 1542
Hin1I GRCGYC 1 cut(s) 1797
Hin6I GCGC 3 cut(s) 799, 801, 1540
HinP1I GCGC 3 cut(s) 799, 801, 1540
HincII GTYRAC 1 cut(s) 777
HindII GTYRAC 1 cut(s) 777
HindIII AAGCTT 4 cut(s) 27, 479, 740, 1690
HpaI GTTAAC 1 cut(s) 777
HpaII CCGG 3 cut(s) 288, 833, 1078
HphI GGTGA 5 cut(s) 122, 271, 976, 1124, 1301
Hpy166II GTNNAC 2 cut(s) 777, 2123
Hpy188I TCNGA 9 cut(s) 273, 421, 630, 712, 784, 1304, 1672, 1736, 1795
Hpy8I GTNNAC 2 cut(s) 777, 2123
Hpy99I CGWCG 2 cut(s) 529, 1799
HpyAV CCTTC 8 cut(s) 268, 437, 555, 602, 1286, 1892, 1907, 2060
HpyCH4IV ACGT 3 cut(s) 183, 367, 1797
HpyF10VI GCNNNNNNNGC 1 cut(s) 2156
HpyF3I CTNAG 9 cut(s) 36, 420, 518, 537, 669, 711, 1510, 1669, 2155
HpySE526I ACGT 3 cut(s) 183, 367, 1797
Hsp92I GRCGYC 1 cut(s) 1797
HspAI GCGC 3 cut(s) 799, 801, 1540
KspAI GTTAAC 1 cut(s) 777
Kzo9I GATC 7 cut(s) 521, 541, 1114, 1213, 1304, 1482, 1618
LmnI GCTCC 2 cut(s) 1052, 1578
Lsp1109I GCAGC 5 cut(s) 57, 95, 808, 1187, 1212
LweI GCATC 7 cut(s) 154, 523, 1017, 1314, 1890, 1927, 1949
MabI ACCWGGT 2 cut(s) 469, 2092
MaeI CTAG 8 cut(s) 146, 975, 1371, 1436, 1596, 1686, 1823, 2180
MaeII ACGT 3 cut(s) 183, 367, 1797
MaeIII GTNAC 8 cut(s) 277, 496, 734, 1402, 1631, 1745, 1957, 2167
MalI GATC 7 cut(s) 523, 543, 1116, 1215, 1306, 1484, 1620
MboI GATC 7 cut(s) 521, 541, 1114, 1213, 1304, 1482, 1618
MfeI CAATTG 3 cut(s) 508, 1272, 1767
MflI RGATCY 4 cut(s) 541, 1213, 1304, 1618
MhlI GDGCHC 3 cut(s) 303, 320, 1575
MlyI GAGTC 3 cut(s) 410, 1374, 1946
MmeI TCCRAC 3 cut(s) 357, 1714, 1818
Mph1103I ATGCAT 2 cut(s) 1897, 1942
MroXI GAANNNNTTC 1 cut(s) 919
MseI TTAA 7 cut(s) 8, 213, 621, 776, 1314, 1554, 1656
MslI CAYNNNNRTG 1 cut(s) 906
MspI CCGG 3 cut(s) 288, 833, 1078
MspR9I CCNGG 7 cut(s) 288, 453, 471, 833, 1079, 1682, 2094
MunI CAATTG 3 cut(s) 508, 1272, 1767
Mva1269I GAATGC 1 cut(s) 1458
MvaI CCWGG 4 cut(s) 453, 471, 1682, 2094
MvnI CGCG 1 cut(s) 801
MwoI GCNNNNNNNGC 1 cut(s) 2156
NciI CCSGG 3 cut(s) 288, 833, 1079
NcoI CCATGG 1 cut(s) 638
NdeII GATC 7 cut(s) 521, 541, 1114, 1213, 1304, 1482, 1618
NlaIV GGNNCC 3 cut(s) 543, 577, 1574
NmeAIII GCCGAG 1 cut(s) 2055
NmuCI GTSAC 3 cut(s) 277, 1957, 2167
NsiI ATGCAT 2 cut(s) 1897, 1942
NspI RCATGY 4 cut(s) 1753, 1895, 2038, 2154
PaeI GCATGC 1 cut(s) 2154
PaeR7I CTCGAG 1 cut(s) 1566
PauI GCGCGC 1 cut(s) 799
PceI AGGCCT 1 cut(s) 1921
PciI ACATGT 1 cut(s) 1749
PctI GAATGC 1 cut(s) 1458
PdmI GAANNNNTTC 1 cut(s) 919
PfeI GAWTC 7 cut(s) 355, 377, 1432, 1592, 1854, 1984, 2014
PflFI GACNNNGTC 1 cut(s) 1955
PflMI CCANNNNNTGG 2 cut(s) 225, 644
PfoI TCCNGGA 1 cut(s) 1077
PkrI GCNGC 7 cut(s) 72, 85, 798, 1174, 1177, 1202, 2044
PleI GAGTC 3 cut(s) 410, 1374, 1946
PpsI GAGTC 3 cut(s) 410, 1374, 1946
Ppu21I YACGTR 1 cut(s) 184
PscI ACATGT 1 cut(s) 1749
PshBI ATTAAT 1 cut(s) 1554
Psp6I CCWGG 4 cut(s) 451, 469, 1680, 2092
PspEI GGTNACC 1 cut(s) 277
PspGI CCWGG 4 cut(s) 451, 469, 1680, 2092
PspN4I GGNNCC 3 cut(s) 543, 577, 1574
PspPI GGNCC 3 cut(s) 327, 575, 635
PspXI VCTCGAGB 1 cut(s) 1566
PsuI RGATCY 4 cut(s) 541, 1213, 1304, 1618
PsyI GACNNNGTC 1 cut(s) 1955
PteI GCGCGC 1 cut(s) 799
RsaI GTAC 7 cut(s) 182, 197, 562, 728, 956, 1109, 1321
RsaNI GTAC 7 cut(s) 181, 196, 561, 727, 955, 1108, 1320
RseI CAYNNNNRTG 1 cut(s) 906
SaqAI TTAA 7 cut(s) 8, 213, 621, 776, 1314, 1554, 1656
SatI GCNGC 7 cut(s) 71, 84, 797, 1173, 1176, 1201, 2043
Sau3AI GATC 7 cut(s) 521, 541, 1114, 1213, 1304, 1482, 1618
Sau96I GGNCC 3 cut(s) 327, 575, 635
ScaI AGTACT 1 cut(s) 956
SchI GAGTC 3 cut(s) 410, 1374, 1946
ScrFI CCNGG 7 cut(s) 288, 453, 471, 833, 1079, 1682, 2094
SduI GDGCHC 3 cut(s) 303, 320, 1575
SexAI ACCWGGT 2 cut(s) 469, 2092
SfaNI GCATC 7 cut(s) 154, 523, 1017, 1314, 1890, 1927, 1949
SfcI CTRYAG 4 cut(s) 117, 730, 1245, 1801
Sfr274I CTCGAG 1 cut(s) 1566
SinI GGWCC 3 cut(s) 327, 575, 635
SlaI CTCGAG 1 cut(s) 1566
SmiMI CAYNNNNRTG 1 cut(s) 906
SmlI CTYRAG 3 cut(s) 549, 1013, 1566
SmoI CTYRAG 3 cut(s) 549, 1013, 1566
SpeI ACTAGT 1 cut(s) 2179
SphI GCATGC 1 cut(s) 2154
SseBI AGGCCT 1 cut(s) 1921
SsiI CCGC 3 cut(s) 1173, 1296, 2043
SspMI CTAG 8 cut(s) 146, 975, 1371, 1436, 1596, 1686, 1823, 2180
StuI AGGCCT 1 cut(s) 1921
StyD4I CCNGG 7 cut(s) 286, 451, 469, 831, 1077, 1680, 2092
StyI CCWWGG 1 cut(s) 638
TaiI ACGT 3 cut(s) 186, 370, 1800
TaqI TCGA 9 cut(s) 341, 527, 764, 1567, 1926, 1943, 2127, 2184, 2191
TaqII GACCGA 2 cut(s) 604, 2123
TatI WGTACW 2 cut(s) 726, 954
TauI GCSGC 2 cut(s) 1175, 2045
TfiI GAWTC 7 cut(s) 355, 377, 1432, 1592, 1854, 1984, 2014
Tru1I TTAA 7 cut(s) 8, 213, 621, 776, 1314, 1554, 1656
Tru9I TTAA 7 cut(s) 8, 213, 621, 776, 1314, 1554, 1656
TscAI CASTG 7 cut(s) 259, 664, 940, 1141, 1353, 1654, 2110
TseFI GTSAC 3 cut(s) 277, 1957, 2167
TseI GCWGC 5 cut(s) 70, 83, 796, 1175, 1200
Tsp45I GTSAC 3 cut(s) 277, 1957, 2167
TspGWI ACGGA 1 cut(s) 1945
TspRI CASTG 7 cut(s) 259, 664, 940, 1141, 1353, 1654, 2110
Tth111I GACNNNGTC 1 cut(s) 1955
Van91I CCANNNNNTGG 2 cut(s) 225, 644
VpaK11BI GGWCC 3 cut(s) 327, 575, 635
VspI ATTAAT 1 cut(s) 1554
XagI CCTNNNNNAGG 1 cut(s) 549
XapI RAATTY 4 cut(s) 4, 1066, 1366, 1652
XbaI TCTAGA 2 cut(s) 1370, 1595
XceI RCATGY 4 cut(s) 1753, 1895, 2038, 2154
XhoI CTCGAG 1 cut(s) 1566
XmnI GAANNNNTTC 1 cut(s) 919
XspI CTAG 8 cut(s) 146, 975, 1371, 1436, 1596, 1686, 1823, 2180
ZraI GACGTC 1 cut(s) 1798
ZrmI AGTACT 1 cut(s) 956
Zsp2I ATGCAT 2 cut(s) 1897, 1942
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.