RchiOBHm_Chr5g0026351

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000484
Physical Location & Seq
Forward (+)
20367194 .. 20370751
3558 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ30593

Sequence Viewer

Length: 2643 bp
ATGGAAATGAGGTGTTCGAGTATTACAGTTATGAAAAATCCATTGAAGGTTAGGATGTGCTTACATCCTATTTGCTCTTTTCTGCTTATCATCACAGCGATATTTTCCACAGCGGGAGGCAGCACAAGTACATTTCAGTCCATTAGAGATGGCCAGACTATAGTTTCAACTACTGGAAACTTTGAGCTTGGATTTTTTAGTCCTCCTACTGGTTCTAATAATCGATATGTCGGGATATGGTACAAGAAGATATCGTCTACAATAGTATGGGTTGCCAACAGAGAAGCACCCCTTACTGATTCATCAGGTGTTCTGCAGGTCACCAACCCTGGAATTCTTGTCCTCCTCAACTATACCAAGAGCATAGTTTGGTCCTCAAACACATCAAGATCTGCAGAGAATCCAGTGGCCCAGCTTTTGGATTCAGGAAATCTTGTTGTAACAGATGGCAGTGATACAGACCCTGAGAACTTCCTGTGGCAAAGTTTTGATTACCCAAGTGATACACTCCTACCAGGTATGAAGCTTGGTTGGAACAAAGTTACAGGCTTCAGTAGGCGTCTTACATCATGGAATAGTCCACAGGATCCTTCTCTAGGAAAATATACATTTGAACTTGGTCGCAAAGGATATGGAGAAGAAATTTTGAAGATGGAGTCAGTTATGATATGGCGAACTGGACCATGGAATGGAATCCGATTCAGTGGCTTGCCTCATTTAAGTCCAAACTCCATATTCACATACAATCTTGTTTTAGATGATCATGGTGAAGATTATTATTTTTTTGAGCTTCGTAACAGCTCACTTTATTCAAGATTGACTCTCAATCCAGATGGGCGTGTGCAGCGCTACACATGGATTGATACAAACCAAACTTGGGGATTCCTTTATCAAACAGCTTTAATTGATAACTGCGAAAGTTATGCATTCTGTGGTCCAAATGGTGCATGCTGTATTGACAGTTTCCCAATATGTCGCTGCTTGAATGGATTTCAACCCAAATATCCAAAGGACTGGGATGTAGTGGATTGGTCAAATGGCTGTGTGAGAAGGACTCCACTAAATTGCAGCGGTGACTTGTTTGAGAAGTACTCTGCAGTGAAATTGCCAAACACAGAGCAGTCCTGGTTCAACAAAAGTATGAATCTCAAGGAATGTCAGATGGCGTGCCTGAAGGACTGTTCCTGCACAGCTTATGCAAATTTGGATATCACGGATGGAGGATCTGGCTGCTTGCTGTGGTTTGGCAACCTAATTGATATTAGAAATTTAACTGCAAATGGGCAAGATATTTATATAAGAATGGCTGCATCAGAACAAGGTACAGACTCCTCAACCTCAAACTCAATGTGTAGTATGTTCATGCAAAATTTTCTGAGAGGGACAGTATTGTTCGGTTTACGCCTGGATCATGACAATACAGTTATCAATGCTAAAGACTCTGAATCTAAGAAGGTTAGAATCATACTATGTGGTACTCTGTTGCCTACTGGACTGCTGTTCGTGGGCATAGCCCTGGTATTTTATTGCCGGAAGAAGCAGAGCAAAAAATATGGGAGAATGAGGAGCGGCAAAAAAGAAGGTTCAGAACTTACCTCAGGAGAAGAAGGTTTAGAACTTCCCTCCAAAAAGGAAGATTTTGAACTTCCATTATTTGACTTGGCTACTGTCATTTGTGCCACTGATAACTTCTCAGAGAACAGAAAACTTGGACAAGGTGGATTCGGATCCGTCTTTAAGGGTATATTGCAAGATGGAAAGGAAATAGCTGTGAAGAGGCTCTCGAAGCATTCTAAACAAGGACTGGATGAGTTCAAGAATGAGGTTACACATATTGTCAAACTTCAGCACAGGAATCTAGTGAAGCTTCTGGGATGCTGCATTCAGGAAGATGAAATGATGCTGATCTACGAATGCATGCCTAACAAAAGCTTGGACTTCTTTATTTTTGATCAAAGAAGAAGCTTGTTACTAGATTGGCCTAAGCGCTTTGACATTATTAATGGTATTGCTCGGGGGATCCTTTATCTTCATCAAGATTCTAGACTGAGAGTAATTCATAGAGATCTCAAAGCAGGCAATATCTTATTAGACAATGAATTCAACCCAAAAATCTCAGACTTTGGCCTGGCTAGAAGTTTTGGAGGAAATGAAACTAAAGCAGAAACGAATAAAGTGGTCGGGACATATGGTTACATGTCCCCAGAATATGCAATTGACGGGTTCTACTCGATAAAATCAGACGTGTATAGCTTTGGTGTTTTGATGCTGGAGATAATAAGTGGGAAGAGAAACAGAGGATTCCTTCATCCAGGCCACAACCTCAACCTGCTTGGACATGCATGGAACTTACACACAGAAGGCAGGTCCATTGAATTGCTTGCTACATCAGTAGGGGACTCCATTAATCTACAAGAAGTTCTGCGAACGATTCATGTGGGTCTTTTATGTGTTCAGCAAAATCCACAAGACAGGCCAAGCATGTCAGCTGCAGTACTAAAGTTAAGTGGCGAAGGTGTGCTGCCTGTACCTCAGAAACCTGGTTTTTATAATGAGAGGGCTCTAACTGAACTCCAAGTCCATTCTTCTTCACAATCATGTTCAGTTAATACACTCACTTCTAGCCTATTCGTGCCTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

880

Amino Acids

98.53

Weight (kDa)

7.68

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 87 - 191 3.2e-40 D-mannose binding lectin
S_locus_glycop PF00954 224 - 334 9.5e-28 S-locus glycoprotein domain
PAN_2 PF08276 356 - 421 2e-20 PAN-like domain
Pkinase PF00069 565 - 764 2.4e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 567 - 831 7.1e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2550
Acc36I ACCTGC 3 cut(s) 307, 2337, 2355
AccB7I CCANNNNNTGG 2 cut(s) 418, 689
AccBSI CCGCTC 1 cut(s) 1569
AccI GTMKAC 1 cut(s) 257
AciI CCGC 3 cut(s) 113, 1071, 1569
AclWI GGATC 8 cut(s) 581, 594, 1231, 1416, 1722, 1735, 2014, 2027
AcoI YGGCCR 1 cut(s) 151
AcsI RAATTY 6 cut(s) 333, 642, 1201, 1267, 1369, 2099
AcuI CTGAAG 3 cut(s) 535, 1193, 1829
AcyI GRCGYC 1 cut(s) 559
AfaI GTAC 7 cut(s) 130, 242, 1091, 1324, 1477, 2496, 2529
AfeI AGCGCT 2 cut(s) 848, 1988
AfiI CCNNNNNNNGG 5 cut(s) 209, 418, 596, 689, 877
AflIII ACRYGT 2 cut(s) 2196, 2244
AjiI CACGTC 1 cut(s) 2245
AjnI CCWGG 8 cut(s) 328, 514, 1124, 1404, 1515, 2127, 2311, 2539
AloI GAACNNNNNNTCC 2 cut(s) 2562, 2594
AlwI GGATC 8 cut(s) 581, 594, 1231, 1416, 1722, 1735, 2014, 2027
AlwNI CAGNNNCTG 1 cut(s) 464
Ama87I CYCGRG 1 cut(s) 2013
Aor51HI AGCGCT 2 cut(s) 848, 1988
AoxI GGCC 6 cut(s) 151, 408, 1979, 2125, 2314, 2474
ApeKI GCWGC 9 cut(s) 120, 844, 978, 1068, 1230, 1307, 1878, 2489, 2521
ApoI RAATTY 6 cut(s) 333, 642, 1201, 1267, 1369, 2099
AseI ATTAAT 2 cut(s) 2001, 2406
AspLEI GCGC 2 cut(s) 849, 1989
AspS9I GGNCC 5 cut(s) 372, 409, 680, 935, 2367
AsuHPI GGTGA 3 cut(s) 313, 779, 1085
AvaI CYCGRG 1 cut(s) 2013
AvaII GGWCC 4 cut(s) 372, 680, 935, 2367
AxyI CCTNAGG 1 cut(s) 1597
BalI TGGCCA 1 cut(s) 153
BamHI GGATCC 3 cut(s) 586, 1727, 2019
BanII GRGCYC 1 cut(s) 2563
BbvI GCAGC 9 cut(s) 132, 856, 965, 1080, 1217, 1294, 1865, 2476, 2508
BccI CCATC 7 cut(s) 143, 440, 646, 827, 1156, 1211, 1748
BcgI CGANNNNNNTGC 2 cut(s) 905, 939
BciT130I CCWGG 8 cut(s) 330, 516, 1126, 1406, 1517, 2129, 2313, 2541
BclI TGATCA 2 cut(s) 760, 1951
BfaI CTAG 6 cut(s) 596, 1859, 1973, 2043, 2133, 2622
BfmI CTRYAG 5 cut(s) 159, 314, 393, 1095, 2490
BfoI RGCGCY 2 cut(s) 850, 1990
BfuAI ACCTGC 3 cut(s) 307, 2337, 2355
BglII AGATCT 2 cut(s) 389, 2065
BmcAI AGTACT 2 cut(s) 1091, 2496
Bme1390I CCNGG 8 cut(s) 330, 516, 1126, 1406, 1517, 2129, 2313, 2541
Bme18I GGWCC 4 cut(s) 372, 680, 935, 2367
BmeT110I CYCGRG 1 cut(s) 2013
BmgBI CACGTC 1 cut(s) 2245
BmgT120I GGNCC 5 cut(s) 372, 409, 680, 935, 2367
BmiI GGNNCC 3 cut(s) 588, 1729, 2021
BmrFI CCNGG 8 cut(s) 330, 516, 1126, 1406, 1517, 2129, 2313, 2541
BmrI ACTGGG 1 cut(s) 1024
BmsI GCATC 4 cut(s) 1319, 1865, 1890, 2256
BmuI ACTGGG 1 cut(s) 1024
BplI GAGNNNNNCTC 4 cut(s) 1039, 1071, 1076, 1108
BpmI CTGGAG 1 cut(s) 2291
Bpu10I CCTNAGC 1 cut(s) 1983
BpuEI CTTGAG 1 cut(s) 1133
Bsa29I ATCGAT 1 cut(s) 223
BsaBI GATNNNNATC 2 cut(s) 1726, 1904
BsaHI GRCGYC 1 cut(s) 559
BsaJI CCNNGG 3 cut(s) 328, 683, 1515
Bsc4I CCNNNNNNNGG 5 cut(s) 209, 418, 596, 689, 877
Bse1I ACTGG 7 cut(s) 178, 214, 404, 682, 1019, 1495, 1809
Bse21I CCTNAGG 1 cut(s) 1597
Bse8I GATNNNNATC 2 cut(s) 1726, 1904
BseBI CCWGG 8 cut(s) 330, 516, 1126, 1406, 1517, 2129, 2313, 2541
BseCI ATCGAT 1 cut(s) 223
BseDI CCNNGG 3 cut(s) 328, 683, 1515
BseGI GGATG 7 cut(s) 60, 64, 1024, 1222, 1813, 1880, 2308
BseJI GATNNNNATC 2 cut(s) 1726, 1904
BseLI CCNNNNNNNGG 5 cut(s) 209, 418, 596, 689, 877
BseMII CTCAG 7 cut(s) 456, 1367, 1611, 1707, 2039, 2130, 2546
BseNI ACTGG 7 cut(s) 178, 214, 404, 682, 1019, 1495, 1809
BseRI GAGGAG 3 cut(s) 335, 1321, 1579
BseXI GCAGC 9 cut(s) 132, 856, 965, 1080, 1217, 1294, 1865, 2476, 2508
BseYI CCCAGC 1 cut(s) 411
BsgI GTGCAG 2 cut(s) 863, 1171
BshFI GGCC 6 cut(s) 153, 410, 1981, 2127, 2316, 2476
BshVI ATCGAT 1 cut(s) 223
BsiHKCI CYCGRG 1 cut(s) 2013
BsiSI CCGG 1 cut(s) 1531
BslFI GGGAC 4 cut(s) 1396, 2185, 2197, 2411
BslI CCNNNNNNNGG 5 cut(s) 209, 418, 596, 689, 877
BsmFI GGGAC 4 cut(s) 1396, 2185, 2197, 2411
BsmI GAATGC 4 cut(s) 926, 1789, 1881, 1919
BsnI GGCC 6 cut(s) 153, 410, 1981, 2127, 2316, 2476
BsoBI CYCGRG 1 cut(s) 2013
Bsp1286I GDGCHC 1 cut(s) 2563
Bsp19I CCATGG 1 cut(s) 683
BspACI CCGC 3 cut(s) 113, 1071, 1569
BspANI GGCC 6 cut(s) 153, 410, 1981, 2127, 2316, 2476
BspCNI CTCAG 7 cut(s) 457, 1368, 1610, 1706, 2040, 2129, 2545
BspDI ATCGAT 1 cut(s) 223
BspHI TCATGA 1 cut(s) 1411
BspLI GGNNCC 3 cut(s) 588, 1729, 2021
BspMAI CTGCAG 4 cut(s) 318, 397, 1099, 2494
BspMI ACCTGC 3 cut(s) 307, 2337, 2355
BspPI GGATC 8 cut(s) 581, 594, 1231, 1416, 1722, 1735, 2014, 2027
BsrBI CCGCTC 1 cut(s) 1569
BsrI ACTGG 7 cut(s) 178, 214, 404, 682, 1019, 1495, 1809
BssECI CCNNGG 3 cut(s) 328, 683, 1515
BssNI GRCGYC 1 cut(s) 559
BssT1I CCWWGG 1 cut(s) 683
Bst2UI CCWGG 8 cut(s) 330, 516, 1126, 1406, 1517, 2129, 2313, 2541
Bst4CI ACNGT 6 cut(s) 28, 962, 1181, 1387, 1423, 1669
Bst6I CTCTTC 2 cut(s) 1769, 2282
BstACI GRCGYC 1 cut(s) 559
BstC8I GCNNGC 7 cut(s) 710, 949, 1168, 1235, 1919, 2077, 2382
BstDEI CTNAG 9 cut(s) 465, 1376, 1449, 1597, 1693, 1983, 2048, 2116, 2532
BstDSI CCRYGG 1 cut(s) 683
BstEII GGTNACC 1 cut(s) 319
BstENI CCTNNNNNAGG 1 cut(s) 594
BstF5I GGATG 7 cut(s) 60, 64, 1024, 1222, 1813, 1880, 2308
BstH2I RGCGCY 2 cut(s) 850, 1990
BstHHI GCGC 2 cut(s) 849, 1989
BstMWI GCNNNNNNNGC 2 cut(s) 844, 1786
BstNI CCWGG 8 cut(s) 330, 516, 1126, 1406, 1517, 2129, 2313, 2541
BstNSI RCATGY 5 cut(s) 951, 1921, 2200, 2342, 2485
BstPI GGTNACC 1 cut(s) 319
BstSCI CCNGG 8 cut(s) 328, 514, 1124, 1404, 1515, 2127, 2311, 2539
BstSFI CTRYAG 5 cut(s) 159, 314, 393, 1095, 2490
BstV1I GCAGC 9 cut(s) 132, 856, 965, 1080, 1217, 1294, 1865, 2476, 2508
BstX2I RGATCY 6 cut(s) 389, 586, 1223, 1727, 2019, 2065
BstXI CCANNNNNNTGG 1 cut(s) 1014
BstYI RGATCY 6 cut(s) 389, 586, 1223, 1727, 2019, 2065
Bsu15I ATCGAT 1 cut(s) 223
Bsu36I CCTNAGG 1 cut(s) 1597
BsuRI GGCC 6 cut(s) 153, 410, 1981, 2127, 2316, 2476
BsuTUI ATCGAT 1 cut(s) 223
BtgI CCRYGG 1 cut(s) 683
BtrI CACGTC 1 cut(s) 2245
BtsCI GGATG 7 cut(s) 60, 64, 1024, 1222, 1813, 1880, 2308
BtsI GCAGTG 2 cut(s) 457, 1104
BtsIMutI CAGTG 5 cut(s) 411, 457, 709, 1104, 1680
BveI ACCTGC 3 cut(s) 307, 2337, 2355
Cac8I GCNNGC 7 cut(s) 710, 949, 1168, 1235, 1919, 2077, 2382
CaiI CAGNNNCTG 1 cut(s) 464
CciI TCATGA 1 cut(s) 1411
CfoI GCGC 2 cut(s) 849, 1989
Cfr13I GGNCC 5 cut(s) 372, 409, 680, 935, 2367
ClaI ATCGAT 1 cut(s) 223
CseI GACGC 1 cut(s) 548
CsiI ACCWGGT 2 cut(s) 514, 2539
Csp6I GTAC 7 cut(s) 129, 241, 1090, 1323, 1476, 2495, 2528
CviQI GTAC 7 cut(s) 129, 241, 1090, 1323, 1476, 2495, 2528
DdeI CTNAG 9 cut(s) 465, 1376, 1449, 1597, 1693, 1983, 2048, 2116, 2532
EaeI YGGCCR 1 cut(s) 151
Eam1104I CTCTTC 2 cut(s) 1769, 2282
EarI CTCTTC 2 cut(s) 1769, 2282
Eco130I CCWWGG 1 cut(s) 683
Eco24I GRGCYC 1 cut(s) 2563
Eco32I GATATC 2 cut(s) 252, 1210
Eco47I GGWCC 4 cut(s) 372, 680, 935, 2367
Eco47III AGCGCT 2 cut(s) 848, 1988
Eco57I CTGAAG 3 cut(s) 535, 1193, 1829
Eco81I CCTNAGG 1 cut(s) 1597
Eco88I CYCGRG 1 cut(s) 2013
Eco91I GGTNACC 1 cut(s) 319
EcoNI CCTNNNNNAGG 1 cut(s) 594
EcoO65I GGTNACC 1 cut(s) 319
EcoRI GAATTC 2 cut(s) 333, 2099
EcoRII CCWGG 8 cut(s) 328, 514, 1124, 1404, 1515, 2127, 2311, 2539
EcoRV GATATC 2 cut(s) 252, 1210
EcoT14I CCWWGG 1 cut(s) 683
EcoT22I ATGCAT 3 cut(s) 928, 1919, 2344
EcoT38I GRGCYC 1 cut(s) 2563
ErhI CCWWGG 1 cut(s) 683
FalI AAGNNNNNCTT 2 cut(s) 276, 308
FaqI GGGAC 4 cut(s) 1396, 2185, 2197, 2411
FauI CCCGC 1 cut(s) 106
FauNDI CATATG 1 cut(s) 2188
FbaI TGATCA 2 cut(s) 760, 1951
FblI GTMKAC 1 cut(s) 257
FokI GGATG 7 cut(s) 51, 67, 1031, 1229, 1820, 1887, 2295
FriOI GRGCYC 1 cut(s) 2563
FspBI CTAG 6 cut(s) 596, 1859, 1973, 2043, 2133, 2622
GlaI GCGC 2 cut(s) 848, 1988
GsaI CCCAGC 1 cut(s) 415
GsuI CTGGAG 1 cut(s) 2291
HaeII RGCGCY 2 cut(s) 850, 1990
HaeIII GGCC 6 cut(s) 153, 410, 1981, 2127, 2316, 2476
HapII CCGG 1 cut(s) 1531
HgaI GACGC 1 cut(s) 548
HhaI GCGC 2 cut(s) 849, 1989
Hin1I GRCGYC 1 cut(s) 559
Hin6I GCGC 2 cut(s) 847, 1987
HinP1I GCGC 2 cut(s) 847, 1987
HindIII AAGCTT 4 cut(s) 524, 1865, 1930, 1963
HpaII CCGG 1 cut(s) 1531
HphI GGTGA 3 cut(s) 313, 779, 1085
Hpy166II GTNNAC 3 cut(s) 258, 581, 1400
Hpy8I GTNNAC 3 cut(s) 258, 581, 1400
HpyCH4III ACNGT 6 cut(s) 28, 962, 1181, 1387, 1423, 1669
HpyCH4IV ACGT 1 cut(s) 2244
HpyF10VI GCNNNNNNNGC 2 cut(s) 844, 1786
HpyF3I CTNAG 9 cut(s) 465, 1376, 1449, 1597, 1693, 1983, 2048, 2116, 2532
HpySE526I ACGT 1 cut(s) 2244
Hsp92I GRCGYC 1 cut(s) 559
HspAI GCGC 2 cut(s) 847, 1987
Ksp22I TGATCA 2 cut(s) 760, 1951
LmnI GCTCC 1 cut(s) 1566
Lsp1109I GCAGC 9 cut(s) 132, 856, 965, 1080, 1217, 1294, 1865, 2476, 2508
LweI GCATC 4 cut(s) 1319, 1865, 1890, 2256
MabI ACCWGGT 2 cut(s) 514, 2539
MaeI CTAG 6 cut(s) 596, 1859, 1973, 2043, 2133, 2622
MaeII ACGT 1 cut(s) 2244
MaeIII GTNAC 8 cut(s) 319, 439, 541, 794, 1073, 1825, 1968, 2192
MbiI CCGCTC 1 cut(s) 1569
MfeI CAATTG 1 cut(s) 2214
MflI RGATCY 6 cut(s) 389, 586, 1223, 1727, 2019, 2065
MhlI GDGCHC 1 cut(s) 2563
MlsI TGGCCA 1 cut(s) 153
MluNI TGGCCA 1 cut(s) 153
MlyI GAGTC 6 cut(s) 665, 814, 1048, 1322, 1433, 2393
MmeI TCCRAC 1 cut(s) 512
Mox20I TGGCCA 1 cut(s) 153
Mph1103I ATGCAT 3 cut(s) 928, 1919, 2344
MscI TGGCCA 1 cut(s) 153
MseI TTAA 9 cut(s) 719, 902, 1271, 1737, 2001, 2406, 2504, 2607, 2641
MslI CAYNNNNRTG 1 cut(s) 2596
Msp20I TGGCCA 1 cut(s) 153
MspA1I CMGCKG 3 cut(s) 113, 1071, 2489
MspI CCGG 1 cut(s) 1531
MspR9I CCNGG 8 cut(s) 330, 516, 1126, 1406, 1517, 2129, 2313, 2541
MunI CAATTG 1 cut(s) 2214
Mva1269I GAATGC 4 cut(s) 926, 1789, 1881, 1919
MvaI CCWGG 8 cut(s) 330, 516, 1126, 1406, 1517, 2129, 2313, 2541
MwoI GCNNNNNNNGC 2 cut(s) 844, 1786
NcoI CCATGG 1 cut(s) 683
NdeI CATATG 1 cut(s) 2188
NlaIV GGNNCC 3 cut(s) 588, 1729, 2021
NmuCI GTSAC 2 cut(s) 319, 1073
NsiI ATGCAT 3 cut(s) 928, 1919, 2344
NspI RCATGY 5 cut(s) 951, 1921, 2200, 2342, 2485
PaeI GCATGC 2 cut(s) 951, 1921
PagI TCATGA 1 cut(s) 1411
PciI ACATGT 1 cut(s) 2196
PctI GAATGC 4 cut(s) 926, 1789, 1881, 1919
PflMI CCANNNNNTGG 2 cut(s) 418, 689
PleI GAGTC 6 cut(s) 664, 814, 1048, 1322, 1433, 2393
PpsI GAGTC 6 cut(s) 664, 814, 1048, 1322, 1433, 2393
PscI ACATGT 1 cut(s) 2196
PshBI ATTAAT 2 cut(s) 2001, 2406
PsiI TTATAA 1 cut(s) 2550
Psp6I CCWGG 8 cut(s) 328, 514, 1124, 1404, 1515, 2127, 2311, 2539
PspEI GGTNACC 1 cut(s) 319
PspFI CCCAGC 1 cut(s) 411
PspGI CCWGG 8 cut(s) 328, 514, 1124, 1404, 1515, 2127, 2311, 2539
PspN4I GGNNCC 3 cut(s) 588, 1729, 2021
PspPI GGNCC 5 cut(s) 372, 409, 680, 935, 2367
PstI CTGCAG 4 cut(s) 318, 397, 1099, 2494
PstNI CAGNNNCTG 1 cut(s) 464
PsuI RGATCY 6 cut(s) 389, 586, 1223, 1727, 2019, 2065
PvuII CAGCTG 1 cut(s) 2489
RsaI GTAC 7 cut(s) 130, 242, 1091, 1324, 1477, 2496, 2529
RsaNI GTAC 7 cut(s) 129, 241, 1090, 1323, 1476, 2495, 2528
RseI CAYNNNNRTG 1 cut(s) 2596
SaqAI TTAA 9 cut(s) 719, 902, 1271, 1737, 2001, 2406, 2504, 2607, 2641
Sau96I GGNCC 5 cut(s) 372, 409, 680, 935, 2367
ScaI AGTACT 2 cut(s) 1091, 2496
SchI GAGTC 6 cut(s) 665, 814, 1048, 1322, 1433, 2393
ScrFI CCNGG 8 cut(s) 330, 516, 1126, 1406, 1517, 2129, 2313, 2541
SduI GDGCHC 1 cut(s) 2563
SexAI ACCWGGT 2 cut(s) 514, 2539
SfaNI GCATC 4 cut(s) 1319, 1865, 1890, 2256
SfcI CTRYAG 5 cut(s) 159, 314, 393, 1095, 2490
SinI GGWCC 4 cut(s) 372, 680, 935, 2367
SmiMI CAYNNNNRTG 1 cut(s) 2596
SmlI CTYRAG 1 cut(s) 1148
SmoI CTYRAG 1 cut(s) 1148
SphI GCATGC 2 cut(s) 951, 1921
SsiI CCGC 3 cut(s) 113, 1071, 1569
SspMI CTAG 6 cut(s) 596, 1859, 1973, 2043, 2133, 2622
StyD4I CCNGG 8 cut(s) 328, 514, 1124, 1404, 1515, 2127, 2311, 2539
StyI CCWWGG 1 cut(s) 683
TaaI ACNGT 6 cut(s) 28, 962, 1181, 1387, 1423, 1669
TaiI ACGT 1 cut(s) 2247
TaqI TCGA 4 cut(s) 17, 223, 1784, 2231
TatI WGTACW 3 cut(s) 128, 1089, 2494
TauI GCSGC 1 cut(s) 1572
Tru1I TTAA 9 cut(s) 719, 902, 1271, 1737, 2001, 2406, 2504, 2607, 2641
Tru9I TTAA 9 cut(s) 719, 902, 1271, 1737, 2001, 2406, 2504, 2607, 2641
TscAI CASTG 5 cut(s) 411, 457, 709, 1104, 1687
TseFI GTSAC 2 cut(s) 319, 1073
TseI GCWGC 9 cut(s) 120, 844, 978, 1068, 1230, 1307, 1878, 2489, 2521
Tsp45I GTSAC 2 cut(s) 319, 1073
TspGWI ACGGA 2 cut(s) 1229, 1720
TspRI CASTG 5 cut(s) 411, 457, 709, 1104, 1687
Van91I CCANNNNNTGG 2 cut(s) 418, 689
VpaK11BI GGWCC 4 cut(s) 372, 680, 935, 2367
VspI ATTAAT 2 cut(s) 2001, 2406
XagI CCTNNNNNAGG 1 cut(s) 594
XapI RAATTY 6 cut(s) 333, 642, 1201, 1267, 1369, 2099
XbaI TCTAGA 1 cut(s) 2042
XceI RCATGY 5 cut(s) 951, 1921, 2200, 2342, 2485
XmiI GTMKAC 1 cut(s) 257
XspI CTAG 6 cut(s) 596, 1859, 1973, 2043, 2133, 2622
ZrmI AGTACT 2 cut(s) 1091, 2496
Zsp2I ATGCAT 3 cut(s) 928, 1919, 2344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.