RLG00000032934

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000484
Physical Location & Seq
Reverse (-)
21162757 .. 21166776
4020 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032934

Sequence Viewer

Length: 2184 bp
ATGACACACACAAGTACCTTTCAGTTCCTCAGAGATGGCCAGACCCATGTTTCAACTGGTGGAACCTTTGAGCTTGGATTTTTCACTCCTCAAGGTGCTTCTAATAAGCGATATGTTGGAATATGGTACAAGAATATACCCGTTAACACTGTTGTATGGGTTGCCAACAGAGACACGCCCCTCACTGACTCATCAGGTGTTCTGAAGATCACCAGTCCTGGAATTCTTGTCCTTGTCGACCATAACAAGACAGTTTGGTCCTCCAACACTTCAAGTCCAGCACAGAATCCAGTGGCACTGCTTTTGGATTCAGGAAATCTTGTTGTGACAGATAAGAGTGACAATGTTGACCCATCTGCAGAGAGCTTTCTGTGGCAAAGTTTTGACCACCCAGGTAATACATTGCTACCAGGTATGAAACTTGGTAGAAACACAATTACAGGTTTCAAATGGTTTATGTCATCATGGAAAAGTCCCCAGGATCCTTCCCAAGGCAAATATACATTTCAACATGGTCCCAAAGGATATGCAGAGCAGATTATGATGGAGGGTTCGGTCATGAAATTTCGGACTGGACCATGGAATGGAATCCGCTTCAGTGGAGCGCCTCATTTAAGTCCAAATCCAATATACACATACAATCTTCTTTTTGATCATGATGAAGAATATTATTCTTATAAGCTTCTTAACAGGTCAATTCTTTCCAGGCTGGTGTTGGCTCAAGATGGGCTTGTGCACCGATACACATGGATTGATAGAACCCGAGAATGGTTCCTTCATCTAACAGCACAACTTGATAGCTGTGATACCTATGCACTTTGTGGTGCCTATGGTGTATGTAATATTGAGAACTCCCCAGCATGTGGGTGCTTGAAAGGATTTTCACCTAAATATCCGAAAGATTGGGAAGTGATGGATTGGTCAAATGGTTGTGTCAGGAAGACTCCACTGAGTTGCAATGGAGACATATTTCAAAAGTACTATGGGGTGAAATTGCCAAACACAGAACAATCCTGGTTCAACACAAGTATGAACATCAAGGAATGTGAAATGATGTGCACAAAGAACTGTTCCTGCACAGCTTATTCAAATTTATATATCAGCCAAGGAGGGACTGGGTGCCGGATGTGGTTTGGTGACCTGATTGATATTAGTATTAGAAATTTCACTAAAAATGGGGAAGATCTCTATATACGAATGGCCGCATCAGAACTACCTACAGAGCAGCTCCGGATTTTTGAGGGTTTTGAGGTTTTTTATTTTGACATTTCCATTCCAGGTATGTTGAGAGATGGAGGTGAAGTAGCTGTGAAAAGGCTCTCAAAGCATTCTAAACAAGGACTAGATGAATTCAAGAATGAGGTTATGCATATTGCAAAACTTCAGCACAGGAATCTAGTGAAGCTTCACGGATGCTACATTCAAGAAGATGAAATGATGCTGATCTATGAATTCATGCCCAACAAAAGCTTGGACTTCTTTATTTTTGATCATACAAAACGCATGTCACTAGACTGGCGTATGCGGTTTGACATTATTAATGGCATTGCTCGAGGGCTCCTCTATCTTCATCAAGACTCCAGACTCAGAGTAATTCATCGAGATCTCAAAGCAGGAAATATTTTATTAGACAGTGAATTTATCCCAAAAATATCTGACTTTGGCCTGGCTAGAAGTTTGGGAGGAAATGAAACTAAAGCAGAAACGAACAGAGTGGTTGGCACGTATGGTTTTATGTCTCCAGAATATGCAATTGATGGGTTCTACTCCACCAAATCCGACGTCTTTAGCTTTGGTGTTTCGGTGTTGGAGATAATAAGTGGGAAGAGAAACAGAGGATTCTATAATCCAGACCACAGCCTAAACCTCCTTGGACATGCATGGAAGCTTTACACACAAGGCAGGTCCATTGAACTGCTTGATACATCAGTAGGGGACTCGACTAATCTACTAGAGGAAGTTCTGCGATCGGTTCATGTAGGACTCTTGTGTGTGCAGCATAATCCAGAAGATAGGCCAAGCATGTCAGCTGCTGTTCTAATGTTGAGTGGTGAAGGTGCATTGCCTCAACCCCAGAAACCAGGTTTTTATACTGAAACGAATCTGAGTGAGATCAAAGTCGACGCCGCTCCTAAAGCATGCTCAGCTAATGAAGTCACTTTTACACTCTTGGAGGCCCGATAA

Protein Analysis

728

Amino Acids

82.09

Weight (kDa)

6.39

Isoelectric Point (pI)

44.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 50 - 157 4.9e-36 D-mannose binding lectin
S_locus_glycop PF00954 189 - 297 2.6e-31 S-locus glycoprotein domain
PAN_2 PF08276 319 - 384 1.2e-18 PAN-like domain
Pkinase PF00069 431 - 613 5.4e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 433 - 678 6.1e-41 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 685 - 727 1.7e-07 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 678
AasI GACNNNNNNGTC 1 cut(s) 256
AatII GACGTC 1 cut(s) 1785
Acc36I ACCTGC 1 cut(s) 1893
AccB1I GGYRCC 2 cut(s) 824, 1119
AccB7I CCANNNNNTGG 2 cut(s) 584, 863
AccBSI CCGCTC 1 cut(s) 2129
AccI GTMKAC 2 cut(s) 237, 2121
AccIII TCCGGA 1 cut(s) 1230
AciI CCGC 4 cut(s) 592, 1203, 1525, 2127
AclWI GGATC 2 cut(s) 476, 489
AcoI YGGCCR 2 cut(s) 37, 1200
AcsI RAATTY 7 cut(s) 222, 563, 1090, 1162, 1349, 1451, 1637
AcuI CTGAAG 3 cut(s) 224, 580, 1367
AcyI GRCGYC 2 cut(s) 1782, 2124
AdeI CACNNNGTG 1 cut(s) 821
AfaI GTAC 3 cut(s) 16, 128, 980
AfiI CCNNNNNNNGG 4 cut(s) 491, 584, 768, 863
AjnI CCWGG 9 cut(s) 217, 391, 409, 477, 704, 1013, 1276, 1665, 2080
Alw21I GWGCWC 2 cut(s) 738, 1061
Alw26I GTCTC 3 cut(s) 165, 957, 1743
Alw44I GTGCAC 2 cut(s) 734, 1057
AlwI GGATC 2 cut(s) 476, 489
AlwNI CAGNNNCTG 1 cut(s) 2033
Ama87I CYCGRG 2 cut(s) 762, 1551
Aor13HI TCCGGA 1 cut(s) 1230
AoxI GGCC 5 cut(s) 37, 1200, 1663, 2015, 2175
ApaLI GTGCAC 2 cut(s) 734, 1057
ApeKI GCWGC 3 cut(s) 1225, 1996, 2030
ApoI RAATTY 7 cut(s) 222, 563, 1090, 1162, 1349, 1451, 1637
ArsI GACNNNNNNTTYG 4 cut(s) 1490, 1522, 1767, 1799
AseI ATTAAT 1 cut(s) 1539
AspLEI GCGC 1 cut(s) 607
AspS9I GGNCC 5 cut(s) 258, 515, 575, 1905, 2176
AsuHPI GGTGA 6 cut(s) 202, 876, 1000, 1148, 1310, 2063
AvaI CYCGRG 2 cut(s) 762, 1551
AvaII GGWCC 4 cut(s) 258, 515, 575, 1905
BaeGI GKGCMC 2 cut(s) 738, 1061
BaeI ACNNNNGTAYC 3 cut(s) 31, 1914, 1947
BalI TGGCCA 1 cut(s) 39
BamHI GGATCC 1 cut(s) 481
BanI GGYRCC 2 cut(s) 824, 1119
BanII GRGCYC 1 cut(s) 1560
BbsI GAAGAC 1 cut(s) 947
Bbv12I GWGCWC 2 cut(s) 738, 1061
BbvI GCAGC 3 cut(s) 1237, 2008, 2017
BccI CCATC 7 cut(s) 29, 361, 538, 719, 907, 1286, 1751
BciT130I CCWGG 9 cut(s) 219, 393, 411, 479, 706, 1015, 1278, 1667, 2082
BclI TGATCA 2 cut(s) 652, 1489
BcoDI GTCTC 3 cut(s) 165, 957, 1743
BfaI CTAG 5 cut(s) 1343, 1397, 1511, 1671, 1952
BfmI CTRYAG 2 cut(s) 357, 1218
BfoI RGCGCY 1 cut(s) 608
BfuAI ACCTGC 1 cut(s) 1893
BglII AGATCT 2 cut(s) 1183, 1603
BisI GCNGC 5 cut(s) 1203, 1226, 1997, 2031, 2127
BlpI GCTNAGC 1 cut(s) 2143
BlsI GCNGC 5 cut(s) 1204, 1227, 1998, 2032, 2128
BmcAI AGTACT 1 cut(s) 980
Bme1390I CCNGG 9 cut(s) 219, 393, 411, 479, 706, 1015, 1278, 1667, 2082
Bme18I GGWCC 4 cut(s) 258, 515, 575, 1905
BmeT110I CYCGRG 2 cut(s) 762, 1551
BmgT120I GGNCC 5 cut(s) 258, 515, 575, 1905, 2176
BmiI GGNNCC 7 cut(s) 64, 483, 517, 773, 826, 1121, 1559
BmrFI CCNGG 9 cut(s) 219, 393, 411, 479, 706, 1015, 1278, 1667, 2082
BmrI ACTGGG 1 cut(s) 1125
BmsI GCATC 3 cut(s) 1214, 1403, 1428
BmuI ACTGGG 1 cut(s) 1125
BpiI GAAGAC 1 cut(s) 947
BplI GAGNNNNNCTC 2 cut(s) 1545, 1577
BpmI CTGGAG 2 cut(s) 1564, 1725
Bpu1102I GCTNAGC 1 cut(s) 2143
BpuEI CTTGAG 2 cut(s) 75, 705
BsaAI YACGTR 1 cut(s) 1725
BsaBI GATNNNNATC 1 cut(s) 1442
BsaHI GRCGYC 2 cut(s) 1782, 2124
BsaJI CCNNGG 6 cut(s) 391, 477, 490, 578, 1105, 1870
BsaWI WCCGGW 1 cut(s) 1230
BsaXI ACNNNNNCTCC 2 cut(s) 1287, 1317
Bsc4I CCNNNNNNNGG 4 cut(s) 491, 584, 768, 863
Bse1I ACTGG 6 cut(s) 61, 213, 290, 577, 1120, 1520
Bse3DI GCAATG 4 cut(s) 401, 964, 1545, 2060
Bse8I GATNNNNATC 1 cut(s) 1442
BseAI TCCGGA 1 cut(s) 1230
BseBI CCWGG 9 cut(s) 219, 393, 411, 479, 706, 1015, 1278, 1667, 2082
BseDI CCNNGG 6 cut(s) 391, 477, 490, 578, 1105, 1870
BseGI GGATG 2 cut(s) 1131, 1418
BseJI GATNNNNATC 1 cut(s) 1442
BseLI CCNNNNNNNGG 4 cut(s) 491, 584, 768, 863
BseMI GCAATG 4 cut(s) 401, 964, 1545, 2060
BseMII CTCAG 5 cut(s) 43, 941, 1600, 2096, 2157
BseNI ACTGG 6 cut(s) 61, 213, 290, 577, 1120, 1520
BseRI GAGGAG 2 cut(s) 78, 1550
BseSI GKGCMC 2 cut(s) 738, 1061
BseXI GCAGC 3 cut(s) 1237, 2008, 2017
BseYI CCCAGC 1 cut(s) 856
BsgI GTGCAG 2 cut(s) 1060, 2015
Bsh1285I CGRYCG 1 cut(s) 1970
BshFI GGCC 5 cut(s) 39, 1202, 1665, 2017, 2177
BshNI GGYRCC 2 cut(s) 824, 1119
BsiEI CGRYCG 1 cut(s) 1970
BsiHKAI GWGCWC 2 cut(s) 738, 1061
BsiHKCI CYCGRG 2 cut(s) 762, 1551
BsiSI CCGG 2 cut(s) 1123, 1231
BslFI GGGAC 4 cut(s) 459, 501, 1126, 1949
BslI CCNNNNNNNGG 4 cut(s) 491, 584, 768, 863
BsmAI GTCTC 3 cut(s) 165, 957, 1743
BsmFI GGGAC 4 cut(s) 459, 501, 1126, 1949
BsmI GAATGC 1 cut(s) 1327
BsnI GGCC 5 cut(s) 39, 1202, 1665, 2017, 2177
BsoBI CYCGRG 2 cut(s) 762, 1551
Bsp1286I GDGCHC 3 cut(s) 738, 1061, 1560
Bsp13I TCCGGA 1 cut(s) 1230
Bsp143I GATC 9 cut(s) 207, 481, 652, 1183, 1443, 1489, 1603, 1967, 2112
Bsp1720I GCTNAGC 1 cut(s) 2143
Bsp19I CCATGG 1 cut(s) 578
BspACI CCGC 4 cut(s) 592, 1203, 1525, 2127
BspANI GGCC 5 cut(s) 39, 1202, 1665, 2017, 2177
BspCNI CTCAG 5 cut(s) 42, 942, 1599, 2097, 2156
BspEI TCCGGA 1 cut(s) 1230
BspHI TCATGA 2 cut(s) 558, 655
BspLI GGNNCC 7 cut(s) 64, 483, 517, 773, 826, 1121, 1559
BspMAI CTGCAG 1 cut(s) 361
BspMI ACCTGC 1 cut(s) 1893
BspPI GGATC 2 cut(s) 476, 489
BspT107I GGYRCC 2 cut(s) 824, 1119
BsrBI CCGCTC 1 cut(s) 2129
BsrDI GCAATG 4 cut(s) 401, 964, 1545, 2060
BsrI ACTGG 6 cut(s) 61, 213, 290, 577, 1120, 1520
BssECI CCNNGG 6 cut(s) 391, 477, 490, 578, 1105, 1870
BssMI GATC 9 cut(s) 207, 481, 652, 1183, 1443, 1489, 1603, 1967, 2112
BssNI GRCGYC 2 cut(s) 1782, 2124
BssT1I CCWWGG 4 cut(s) 490, 578, 1105, 1870
Bst2UI CCWGG 9 cut(s) 219, 393, 411, 479, 706, 1015, 1278, 1667, 2082
Bst4CI ACNGT 4 cut(s) 151, 253, 1070, 1634
Bst6I CTCTTC 1 cut(s) 1820
BstACI GRCGYC 2 cut(s) 1782, 2124
BstBAI YACGTR 1 cut(s) 1725
BstC8I GCNNGC 1 cut(s) 2140
BstDEI CTNAG 5 cut(s) 29, 950, 1586, 2105, 2143
BstDSI CCRYGG 1 cut(s) 578
BstEII GGTNACC 1 cut(s) 1136
BstENI CCTNNNNNAGG 1 cut(s) 489
BstF5I GGATG 2 cut(s) 1131, 1418
BstH2I RGCGCY 1 cut(s) 608
BstHHI GCGC 1 cut(s) 607
BstKTI GATC 9 cut(s) 210, 484, 655, 1186, 1446, 1492, 1606, 1970, 2115
BstMAI GTCTC 3 cut(s) 165, 957, 1743
BstMBI GATC 9 cut(s) 207, 481, 652, 1183, 1443, 1489, 1603, 1967, 2112
BstMCI CGRYCG 1 cut(s) 1970
BstMWI GCNNNNNNNGC 3 cut(s) 1324, 2135, 2144
BstNI CCWGG 9 cut(s) 219, 393, 411, 479, 706, 1015, 1278, 1667, 2082
BstNSI RCATGY 5 cut(s) 864, 1507, 1880, 2026, 2142
BstPI GGTNACC 1 cut(s) 1136
BstSCI CCNGG 9 cut(s) 217, 391, 409, 477, 704, 1013, 1276, 1665, 2080
BstSFI CTRYAG 2 cut(s) 357, 1218
BstSLI GKGCMC 2 cut(s) 738, 1061
BstV1I GCAGC 3 cut(s) 1237, 2008, 2017
BstV2I GAAGAC 1 cut(s) 947
BstX2I RGATCY 3 cut(s) 481, 1183, 1603
BstYI RGATCY 3 cut(s) 481, 1183, 1603
BsuRI GGCC 5 cut(s) 39, 1202, 1665, 2017, 2177
BtgI CCRYGG 1 cut(s) 578
BtsCI GGATG 2 cut(s) 1131, 1418
BtsI GCAGTG 1 cut(s) 296
BtsIMutI CAGTG 7 cut(s) 147, 183, 296, 297, 604, 947, 1639
BveI ACCTGC 1 cut(s) 1893
Cac8I GCNNGC 1 cut(s) 2140
CaiI CAGNNNCTG 1 cut(s) 2033
CciI TCATGA 2 cut(s) 558, 655
CfoI GCGC 1 cut(s) 607
Cfr13I GGNCC 5 cut(s) 258, 515, 575, 1905, 2176
CseI GACGC 1 cut(s) 2132
CsiI ACCWGGT 2 cut(s) 409, 2080
Csp6I GTAC 3 cut(s) 15, 127, 979
CviQI GTAC 3 cut(s) 15, 127, 979
DdeI CTNAG 5 cut(s) 29, 950, 1586, 2105, 2143
DpnI GATC 9 cut(s) 209, 483, 654, 1185, 1445, 1491, 1605, 1969, 2114
DpnII GATC 9 cut(s) 207, 481, 652, 1183, 1443, 1489, 1603, 1967, 2112
DraIII CACNNNGTG 1 cut(s) 821
DrdI GACNNNNNNGTC 1 cut(s) 256
DseDI GACNNNNNNGTC 1 cut(s) 256
EaeI YGGCCR 2 cut(s) 37, 1200
Eam1104I CTCTTC 1 cut(s) 1820
EarI CTCTTC 1 cut(s) 1820
Eco130I CCWWGG 4 cut(s) 490, 578, 1105, 1870
Eco24I GRGCYC 1 cut(s) 1560
Eco47I GGWCC 4 cut(s) 258, 515, 575, 1905
Eco57I CTGAAG 3 cut(s) 224, 580, 1367
Eco88I CYCGRG 2 cut(s) 762, 1551
Eco91I GGTNACC 1 cut(s) 1136
EcoNI CCTNNNNNAGG 1 cut(s) 489
EcoO65I GGTNACC 1 cut(s) 1136
EcoRI GAATTC 3 cut(s) 222, 1349, 1451
EcoRII CCWGG 9 cut(s) 217, 391, 409, 477, 704, 1013, 1276, 1665, 2080
EcoT14I CCWWGG 4 cut(s) 490, 578, 1105, 1870
EcoT22I ATGCAT 2 cut(s) 1371, 1882
EcoT38I GRGCYC 1 cut(s) 1560
ErhI CCWWGG 4 cut(s) 490, 578, 1105, 1870
FalI AAGNNNNNCTT 2 cut(s) 714, 746
FaqI GGGAC 4 cut(s) 459, 501, 1126, 1949
FbaI TGATCA 2 cut(s) 652, 1489
FblI GTMKAC 2 cut(s) 237, 2121
Fnu4HI GCNGC 5 cut(s) 1203, 1226, 1997, 2031, 2127
FokI GGATG 2 cut(s) 1138, 1425
FriOI GRGCYC 1 cut(s) 1560
Fsp4HI GCNGC 5 cut(s) 1203, 1226, 1997, 2031, 2127
FspBI CTAG 5 cut(s) 1343, 1397, 1511, 1671, 1952
GlaI GCGC 1 cut(s) 606
GluI GCNGC 5 cut(s) 1203, 1226, 1997, 2031, 2127
GsaI CCCAGC 1 cut(s) 860
GsuI CTGGAG 2 cut(s) 1564, 1725
HaeII RGCGCY 1 cut(s) 608
HaeIII GGCC 5 cut(s) 39, 1202, 1665, 2017, 2177
HapII CCGG 2 cut(s) 1123, 1231
HgaI GACGC 1 cut(s) 2132
HhaI GCGC 1 cut(s) 607
Hin1I GRCGYC 2 cut(s) 1782, 2124
Hin6I GCGC 1 cut(s) 605
HinP1I GCGC 1 cut(s) 605
HincII GTYRAC 4 cut(s) 145, 238, 349, 2122
HindII GTYRAC 4 cut(s) 145, 238, 349, 2122
HindIII AAGCTT 4 cut(s) 680, 1403, 1468, 1886
HpaI GTTAAC 1 cut(s) 145
HpaII CCGG 2 cut(s) 1123, 1231
HphI GGTGA 6 cut(s) 202, 876, 1000, 1148, 1310, 2063
Hpy166II GTNNAC 6 cut(s) 145, 238, 349, 736, 1059, 2122
Hpy188I TCNGA 9 cut(s) 32, 204, 570, 897, 1210, 1589, 1657, 1780, 2106
Hpy8I GTNNAC 6 cut(s) 145, 238, 349, 736, 1059, 2122
Hpy99I CGWCG 2 cut(s) 1784, 2126
HpyAV CCTTC 3 cut(s) 495, 785, 2048
HpyCH4III ACNGT 4 cut(s) 151, 253, 1070, 1634
HpyCH4IV ACGT 2 cut(s) 1724, 1782
HpyF10VI GCNNNNNNNGC 3 cut(s) 1324, 2135, 2144
HpyF3I CTNAG 5 cut(s) 29, 950, 1586, 2105, 2143
HpySE526I ACGT 2 cut(s) 1724, 1782
Hsp92I GRCGYC 2 cut(s) 1782, 2124
HspAI GCGC 1 cut(s) 605
Kpn2I TCCGGA 1 cut(s) 1230
Ksp22I TGATCA 2 cut(s) 652, 1489
KspAI GTTAAC 1 cut(s) 145
Kzo9I GATC 9 cut(s) 207, 481, 652, 1183, 1443, 1489, 1603, 1967, 2112
LmnI GCTCC 4 cut(s) 602, 1233, 1563, 2134
Lsp1109I GCAGC 3 cut(s) 1237, 2008, 2017
LweI GCATC 3 cut(s) 1214, 1403, 1428
MabI ACCWGGT 2 cut(s) 409, 2080
MaeI CTAG 5 cut(s) 1343, 1397, 1511, 1671, 1952
MaeII ACGT 2 cut(s) 1724, 1782
MaeIII GTNAC 5 cut(s) 325, 338, 1136, 1506, 2155
MalI GATC 9 cut(s) 209, 483, 654, 1185, 1445, 1491, 1605, 1969, 2114
MbiI CCGCTC 1 cut(s) 2129
MboI GATC 9 cut(s) 207, 481, 652, 1183, 1443, 1489, 1603, 1967, 2112
MboII GAAGA 9 cut(s) 217, 635, 674, 952, 1193, 1439, 1559, 1837, 2021
MfeI CAATTG 1 cut(s) 1752
MflI RGATCY 3 cut(s) 481, 1183, 1603
MhlI GDGCHC 3 cut(s) 738, 1061, 1560
MlsI TGGCCA 1 cut(s) 39
MluNI TGGCCA 1 cut(s) 39
MlyI GAGTC 6 cut(s) 182, 937, 1571, 1578, 1931, 1977
MmeI TCCRAC 4 cut(s) 97, 288, 1788, 1803
Mox20I TGGCCA 1 cut(s) 39
Mph1103I ATGCAT 2 cut(s) 1371, 1882
MroI TCCGGA 1 cut(s) 1230
MscI TGGCCA 1 cut(s) 39
MseI TTAA 4 cut(s) 144, 614, 687, 1539
MslI CAYNNNNRTG 2 cut(s) 865, 1028
Msp20I TGGCCA 1 cut(s) 39
MspA1I CMGCKG 1 cut(s) 2030
MspI CCGG 2 cut(s) 1123, 1231
MspR9I CCNGG 9 cut(s) 219, 393, 411, 479, 706, 1015, 1278, 1667, 2082
MunI CAATTG 1 cut(s) 1752
Mva1269I GAATGC 1 cut(s) 1327
MvaI CCWGG 9 cut(s) 219, 393, 411, 479, 706, 1015, 1278, 1667, 2082
MwoI GCNNNNNNNGC 3 cut(s) 1324, 2135, 2144
NcoI CCATGG 1 cut(s) 578
NdeII GATC 9 cut(s) 207, 481, 652, 1183, 1443, 1489, 1603, 1967, 2112
NlaIV GGNNCC 7 cut(s) 64, 483, 517, 773, 826, 1121, 1559
NmuCI GTSAC 5 cut(s) 325, 338, 1136, 1506, 2155
NsiI ATGCAT 2 cut(s) 1371, 1882
NspI RCATGY 5 cut(s) 864, 1507, 1880, 2026, 2142
PaeI GCATGC 1 cut(s) 2142
PaeR7I CTCGAG 1 cut(s) 1551
PagI TCATGA 2 cut(s) 558, 655
PctI GAATGC 1 cut(s) 1327
PfeI GAWTC 6 cut(s) 286, 308, 588, 1393, 1839, 2101
PflMI CCANNNNNTGG 2 cut(s) 584, 863
PfoI TCCNGGA 1 cut(s) 217
PkrI GCNGC 5 cut(s) 1204, 1227, 1998, 2032, 2128
Ple19I CGATCG 1 cut(s) 1970
PleI GAGTC 6 cut(s) 182, 937, 1571, 1578, 1931, 1977
PpsI GAGTC 6 cut(s) 182, 937, 1571, 1578, 1931, 1977
Ppu21I YACGTR 1 cut(s) 1725
PshBI ATTAAT 1 cut(s) 1539
PsiI TTATAA 1 cut(s) 678
Psp6I CCWGG 9 cut(s) 217, 391, 409, 477, 704, 1013, 1276, 1665, 2080
PspEI GGTNACC 1 cut(s) 1136
PspFI CCCAGC 1 cut(s) 856
PspGI CCWGG 9 cut(s) 217, 391, 409, 477, 704, 1013, 1276, 1665, 2080
PspN4I GGNNCC 7 cut(s) 64, 483, 517, 773, 826, 1121, 1559
PspPI GGNCC 5 cut(s) 258, 515, 575, 1905, 2176
PspXI VCTCGAGB 1 cut(s) 1551
PstI CTGCAG 1 cut(s) 361
PstNI CAGNNNCTG 1 cut(s) 2033
PsuI RGATCY 3 cut(s) 481, 1183, 1603
PvuI CGATCG 1 cut(s) 1970
PvuII CAGCTG 1 cut(s) 2030
RsaI GTAC 3 cut(s) 16, 128, 980
RsaNI GTAC 3 cut(s) 15, 127, 979
RseI CAYNNNNRTG 2 cut(s) 865, 1028
SalI GTCGAC 2 cut(s) 236, 2120
SaqAI TTAA 4 cut(s) 144, 614, 687, 1539
SatI GCNGC 5 cut(s) 1203, 1226, 1997, 2031, 2127
Sau3AI GATC 9 cut(s) 207, 481, 652, 1183, 1443, 1489, 1603, 1967, 2112
Sau96I GGNCC 5 cut(s) 258, 515, 575, 1905, 2176
ScaI AGTACT 1 cut(s) 980
SchI GAGTC 6 cut(s) 182, 937, 1571, 1578, 1931, 1977
ScrFI CCNGG 9 cut(s) 219, 393, 411, 479, 706, 1015, 1278, 1667, 2082
SduI GDGCHC 3 cut(s) 738, 1061, 1560
SexAI ACCWGGT 2 cut(s) 409, 2080
SfaNI GCATC 3 cut(s) 1214, 1403, 1428
SfcI CTRYAG 2 cut(s) 357, 1218
Sfr274I CTCGAG 1 cut(s) 1551
SinI GGWCC 4 cut(s) 258, 515, 575, 1905
SlaI CTCGAG 1 cut(s) 1551
SmiMI CAYNNNNRTG 2 cut(s) 865, 1028
SmlI CTYRAG 3 cut(s) 90, 720, 1551
SmoI CTYRAG 3 cut(s) 90, 720, 1551
SphI GCATGC 1 cut(s) 2142
SsiI CCGC 4 cut(s) 592, 1203, 1525, 2127
SspI AATATT 3 cut(s) 668, 844, 1621
SspMI CTAG 5 cut(s) 1343, 1397, 1511, 1671, 1952
StyD4I CCNGG 9 cut(s) 217, 391, 409, 477, 704, 1013, 1276, 1665, 2080
StyI CCWWGG 4 cut(s) 490, 578, 1105, 1870
TaaI ACNGT 4 cut(s) 151, 253, 1070, 1634
TaiI ACGT 2 cut(s) 1727, 1785
TaqI TCGA 5 cut(s) 237, 1552, 1600, 1940, 2121
TaqII GACCGA 1 cut(s) 544
TatI WGTACW 1 cut(s) 978
TauI GCSGC 2 cut(s) 1205, 2129
TfiI GAWTC 6 cut(s) 286, 308, 588, 1393, 1839, 2101
Tru1I TTAA 4 cut(s) 144, 614, 687, 1539
Tru9I TTAA 4 cut(s) 144, 614, 687, 1539
TscAI CASTG 7 cut(s) 154, 190, 297, 303, 604, 954, 1639
TseFI GTSAC 5 cut(s) 325, 338, 1136, 1506, 2155
TseI GCWGC 3 cut(s) 1225, 1996, 2030
Tsp45I GTSAC 5 cut(s) 325, 338, 1136, 1506, 2155
TspGWI ACGGA 1 cut(s) 1425
TspRI CASTG 7 cut(s) 154, 190, 297, 303, 604, 954, 1639
Van91I CCANNNNNTGG 2 cut(s) 584, 863
VneI GTGCAC 2 cut(s) 734, 1057
VpaK11BI GGWCC 4 cut(s) 258, 515, 575, 1905
VspI ATTAAT 1 cut(s) 1539
XagI CCTNNNNNAGG 1 cut(s) 489
XapI RAATTY 7 cut(s) 222, 563, 1090, 1162, 1349, 1451, 1637
XceI RCATGY 5 cut(s) 864, 1507, 1880, 2026, 2142
XcmI CCANNNNNNNNNTGG 4 cut(s) 53, 712, 1112, 1468
XhoI CTCGAG 1 cut(s) 1551
XmiI GTMKAC 2 cut(s) 237, 2121
XspI CTAG 5 cut(s) 1343, 1397, 1511, 1671, 1952
ZraI GACGTC 1 cut(s) 1783
ZrmI AGTACT 1 cut(s) 980
Zsp2I ATGCAT 2 cut(s) 1371, 1882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.