pycom05g20370

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000484
Physical Location & Seq
Reverse (-)
23135890 .. 23139197
3308 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g20370.2

Sequence Viewer

Length: 2451 bp
ATGAAGTTTATGAAAAATCCGTCGAAAGCTTCAGCTAAGATGTGCTTACTTCTTTTCTACTCTTCTTTGCTGCTCATTGTCGCAGCAGTATTCTCCACAGCTGATGACGCCACTAGTACATTTCAGTCCATTAGAGACGGTGAGACTGTAGTTTCAACTGGCGGTACGTTTGAGCTAGGATTTTACAGTCCTGATGCTTCTAATAGATGGTATGTGGCGATATGGTACAAGCAGATATCTGTTACAACCATTGTATGGGTTGCCAACAGAGACAAGCCCCTCGCTGATTTGTTCGGCGTTTTGAAGGTCACCAACCCCGGAATTCTCATCCTCAACCACAACATGAGCACAGTTTGGTCCTCCAACACATCGAGAACTGCACAGAATCCAGTGGCACGTCTTTTGGATTCGGGTAATCTTGTCGTGATAGATAGGAGTGATGATGACCCTGAGAACTTTCTGTGGCAAAGTTTTGATTACCCTGGCGATACATTCTTACCAGGTATGAAGATTGGTAGGAACACAGTTACAGGCTTCAATTGTCATCTTAGATCACGGAAAAGTCCTCAGGATCCATCTCAAGGCAATTATACATATCAACTTGGTCCCAAGGGATATGAAGAAATATTTGTGATCCGGTTCAGTGGAACGCCTCAGTTAAATCCAAACCCTATATACACATACGGTCTTGTTTCTGAGCCTAATGAAATGTACTACAGTTACATGCTTCACAACAGCTCAATCTTTTCGAGGGTGGTGTTAACTTCAGATGGACTTGTGCAGCGCTACACATGGATTGATAGAACCAAAGGTTGGGTTCTTTACCTAACAGCCCAAATTGATAACTGTGACAAGTATGCATTCTGTGGTGCAAATGGTGCATGTAACATTGAAAAGTCCTCGGTTTGTAACTGTTTGAAAGGATTTACACCAAAGTTTCCCAAAGAATGGGATTTGATAGATTGGTCTATTGGTTGTGTGAGAGAGACTTCTCCAGATTGCACCGGAGACATGTTCCAAAAGTACTCGGGGTTGAAATTGCCTAGCACAGAACAATCTTGGCTTAACAGAAGTGTGCTCTTCCGATCTCTCAAGGAATGTGAGATGGTGTGCATGAAGAACTGCTCCTGCACGGCTTATACAAATTTGGATATCCGGGATGGAGGAACTGGGTACTTGTTGTGGTACGGCGATCTGATTGATATGAGATACTTTTCTGAAAACGGGCAAGATATTTATATAAGAATGGCCGCAGCAGAACTAGATTATGAAGACGATGCAAAGATCGATGCTAAATACTCTGAATCCAATGCGAAGAAAATGAGAATCATAATAAGCACTACTGTGTTGGCTACCGGACTGCTGATCCTGGGCCTTGCCCTGCTGTTTTTTTTTTGGAAGAAGCAGCACCAAAAAGTTGGAAAACTGGGACACAGCCAAACGGAGGATCTGGAATTACCGTTATTTGACTTGATGACTATAGTTTCTGCAACCAATAACTTTTCAATTGAAAACAAACTTGGTGAAGGCGACGCTGATGGATTTCAGGGTACGATGGAAGATGGACAAGAAATTGCAGTGAAAAGGCTTTCAAAAAGTTCTAGACAAGGACTCGACGAGTTCAAGAATGAAGTTACACATATTGCCAAACTTCAGCACAGGTATCTAGTGAAGCTTCTTGGATGCTGCATTCAAGAAGACGAGATGATACTGATCTACGAGTTCATGCCCAACAAGAGCTTAGACTTCTTTATTTTCAATCAAAGAAGAAGGATGTTATTAGACTGGCCGAAGCGCTTTGATATTATTAATGGGATAGCTCGAGGGCTCCTCTATCTTCATCAAGATTCTAGATTGAGAGTTATTCATAGAGATCTCAAAGCAAGTAACATTTTATTAGGCAGTGAATTTAACCCGAAAATCTCAGACTTTGGCCTGGCTAGAAGCTTTGGAGGAAATGAAACGAAAGCAGAGACGAAGAAAGTGGTTGGAACATACGGTTACATGTCCCCAGAATATGCAATCGATGGTTTCTACTCTATAAAGTCCGACGTCTACAGCTTTGGTGTTATGGTGCTAGAGATAGTGAGTGGGAGCAGAAATAGAGGATTCTCTCATCCAGACCACAAACTCAACCTTCTTGGACATGCATGGATGCTACACACAAAAGGCAGGCCTCTCGAACTGCTTGATTCATCGGTAGAGGACTCCATCACTCTGCTTGAAGTTGTAAGAACTATTCATGTGGGTCTTTTGTGTGTGCAGCGGAATCCAGAGGACAGGCCAAGCATGTCAGCTACAGTTCTAATGTTGAGTGGTGAAGGTGCATTGCCTCAACCTCTAAAACCTGGTTTTTACAGTGAAAGGGATCTAACTGAACTCGAAGCCGGTCATTCTTCTAAAGCATGCTCGGCTAATGAAGTCACTATTTCAGTACTTGAGGCTCGATAA

Protein Analysis

817

Amino Acids

91.96

Weight (kDa)

6.08

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2055
AccB7I CCANNNNNTGG 3 cut(s) 255, 813, 948
AccI GTMKAC 1 cut(s) 2055
AciI CCGC 3 cut(s) 162, 1251, 2266
AclWI GGATC 6 cut(s) 566, 579, 628, 1360, 1455, 2376
AcoI YGGCCR 2 cut(s) 1248, 1787
AcsI RAATTY 3 cut(s) 321, 1144, 1907
AcuI CTGAAG 3 cut(s) 15, 750, 1637
AcyI GRCGYC 2 cut(s) 108, 2052
AfaI GTAC 9 cut(s) 118, 166, 227, 713, 1025, 1175, 1187, 1552, 2436
AfeI AGCGCT 2 cut(s) 785, 1796
AfiI CCNNNNNNNGG 5 cut(s) 255, 581, 813, 948, 1444
AflIII ACRYGT 2 cut(s) 1011, 2004
AhlI ACTAGT 1 cut(s) 113
AjiI CACGTC 1 cut(s) 398
AjnI CCWGG 5 cut(s) 481, 499, 1368, 1935, 2347
AjuI GAANNNNNNNTTGG 4 cut(s) 796, 828, 1503, 1535
AleI CACNNNNGTG 1 cut(s) 1343
Alw21I GWGCWC 2 cut(s) 350, 1080
Alw26I GTCTC 6 cut(s) 129, 137, 264, 980, 1002, 1967
AlwI GGATC 6 cut(s) 566, 579, 628, 1360, 1455, 2376
Ama87I CYCGRG 2 cut(s) 1027, 1821
Aor51HI AGCGCT 2 cut(s) 785, 1796
AoxI GGCC 6 cut(s) 1248, 1372, 1787, 1933, 2174, 2282
ApeKI GCWGC 7 cut(s) 70, 83, 781, 1253, 1405, 1686, 2263
ApoI RAATTY 3 cut(s) 321, 1144, 1907
AseI ATTAAT 1 cut(s) 1809
AspLEI GCGC 2 cut(s) 786, 1797
AspS9I GGNCC 3 cut(s) 357, 605, 1372
AsuC2I CCSGG 2 cut(s) 318, 1157
AsuHPI GGTGA 4 cut(s) 152, 301, 1535, 2330
AvaI CYCGRG 2 cut(s) 1027, 1821
AvaII GGWCC 2 cut(s) 357, 605
AxyI CCTNAGG 1 cut(s) 567
BamHI GGATCC 1 cut(s) 571
BanII GRGCYC 1 cut(s) 1830
BbsI GAAGAC 2 cut(s) 1278, 1704
Bbv12I GWGCWC 2 cut(s) 350, 1080
BbvI GCAGC 7 cut(s) 57, 95, 793, 1265, 1417, 1673, 2275
BceAI ACGGC 2 cut(s) 1149, 1204
BcgI CGANNNNNNTGC 2 cut(s) 2161, 2195
BciT130I CCWGG 5 cut(s) 483, 501, 1370, 1937, 2349
BcnI CCSGG 2 cut(s) 318, 1157
BcoDI GTCTC 6 cut(s) 129, 137, 264, 980, 1002, 1967
BcuI ACTAGT 1 cut(s) 113
BfaI CTAG 9 cut(s) 114, 176, 1044, 1262, 1602, 1667, 1851, 1941, 2078
BfmI CTRYAG 5 cut(s) 147, 715, 1479, 2056, 2298
BfoI RGCGCY 2 cut(s) 787, 1798
BglII AGATCT 1 cut(s) 1873
BisI GCNGC 8 cut(s) 71, 84, 782, 1251, 1254, 1406, 1687, 2264
BlsI GCNGC 8 cut(s) 72, 85, 783, 1252, 1255, 1407, 1688, 2265
BmcAI AGTACT 2 cut(s) 1025, 2436
Bme1390I CCNGG 7 cut(s) 318, 483, 501, 1157, 1370, 1937, 2349
Bme18I GGWCC 2 cut(s) 357, 605
BmeT110I CYCGRG 2 cut(s) 1027, 1821
BmgBI CACGTC 1 cut(s) 398
BmgT120I GGNCC 3 cut(s) 357, 605, 1372
BmiI GGNNCC 3 cut(s) 573, 607, 1829
BmrFI CCNGG 7 cut(s) 318, 483, 501, 1157, 1370, 1937, 2349
BmrI ACTGGG 2 cut(s) 1179, 1436
BmsI GCATC 5 cut(s) 184, 1267, 1279, 1673, 2145
BmuI ACTGGG 2 cut(s) 1179, 1436
BpiI GAAGAC 2 cut(s) 1278, 1704
BplI GAGNNNNNCTC 2 cut(s) 1815, 1847
BpmI CTGGAG 1 cut(s) 978
BpuEI CTTGAG 2 cut(s) 564, 1076
BpuMI CCSGG 2 cut(s) 318, 1157
Bsa29I ATCGAT 2 cut(s) 1287, 2025
BsaBI GATNNNNATC 1 cut(s) 1712
BsaHI GRCGYC 2 cut(s) 108, 2052
BsaJI CCNNGG 5 cut(s) 316, 481, 609, 900, 1369
BsaWI WCCGGW 3 cut(s) 636, 1004, 1355
Bsc4I CCNNNNNNNGG 5 cut(s) 255, 581, 813, 948, 1444
Bse118I RCCGGY 1 cut(s) 2387
Bse1I ACTGG 5 cut(s) 163, 389, 1174, 1431, 1790
Bse21I CCTNAGG 1 cut(s) 567
Bse3DI GCAATG 1 cut(s) 2327
Bse8I GATNNNNATC 1 cut(s) 1712
BseBI CCWGG 5 cut(s) 483, 501, 1370, 1937, 2349
BseCI ATCGAT 2 cut(s) 1287, 2025
BseDI CCNNGG 5 cut(s) 316, 481, 609, 900, 1369
BseGI GGATG 6 cut(s) 327, 1165, 1688, 1779, 2116, 2160
BseJI GATNNNNATC 1 cut(s) 1712
BseLI CCNNNNNNNGG 5 cut(s) 255, 581, 813, 948, 1444
BseMI GCAATG 1 cut(s) 2327
BseMII CTCAG 5 cut(s) 441, 581, 668, 687, 1938
BseNI ACTGG 5 cut(s) 163, 389, 1174, 1431, 1790
BseRI GAGGAG 1 cut(s) 1820
BseXI GCAGC 7 cut(s) 57, 95, 793, 1265, 1417, 1673, 2275
BsgI GTGCAG 4 cut(s) 363, 800, 1114, 2282
BshFI GGCC 6 cut(s) 1250, 1374, 1789, 1935, 2176, 2284
BshVI ATCGAT 2 cut(s) 1287, 2025
BsiHKAI GWGCWC 2 cut(s) 350, 1080
BsiHKCI CYCGRG 2 cut(s) 1027, 1821
BsiSI CCGG 6 cut(s) 318, 637, 1005, 1156, 1356, 2388
BslFI GGGAC 3 cut(s) 591, 1443, 1993
BslI CCNNNNNNNGG 5 cut(s) 255, 581, 813, 948, 1444
BsmAI GTCTC 6 cut(s) 129, 137, 264, 980, 1002, 1967
BsmBI CGTCTC 2 cut(s) 129, 1967
BsmFI GGGAC 3 cut(s) 591, 1443, 1993
BsmI GAATGC 2 cut(s) 860, 1689
BsnI GGCC 6 cut(s) 1250, 1374, 1789, 1935, 2176, 2284
BsoBI CYCGRG 2 cut(s) 1027, 1821
Bsp1286I GDGCHC 3 cut(s) 350, 1080, 1830
BspACI CCGC 3 cut(s) 162, 1251, 2266
BspANI GGCC 6 cut(s) 1250, 1374, 1789, 1935, 2176, 2284
BspCNI CTCAG 5 cut(s) 442, 580, 667, 688, 1937
BspDI ATCGAT 2 cut(s) 1287, 2025
BspLI GGNNCC 3 cut(s) 573, 607, 1829
BspPI GGATC 6 cut(s) 566, 579, 628, 1360, 1455, 2376
BspQI GCTCTTC 1 cut(s) 1085
BsrDI GCAATG 1 cut(s) 2327
BsrFI RCCGGY 1 cut(s) 2387
BsrI ACTGG 5 cut(s) 163, 389, 1174, 1431, 1790
BssAI RCCGGY 1 cut(s) 2387
BssECI CCNNGG 5 cut(s) 316, 481, 609, 900, 1369
BssNI GRCGYC 2 cut(s) 108, 2052
BssT1I CCWWGG 1 cut(s) 609
Bst2UI CCWGG 5 cut(s) 483, 501, 1370, 1937, 2349
Bst6I CTCTTC 2 cut(s) 67, 1085
BstACI GRCGYC 2 cut(s) 108, 2052
BstAPI GCANNNNNTGC 1 cut(s) 878
BstC8I GCNNGC 2 cut(s) 2174, 2407
BstDEI CTNAG 8 cut(s) 36, 450, 548, 567, 654, 696, 1741, 1924
BstEII GGTNACC 1 cut(s) 307
BstF5I GGATG 6 cut(s) 327, 1165, 1688, 1779, 2116, 2160
BstH2I RGCGCY 2 cut(s) 787, 1798
BstHHI GCGC 2 cut(s) 786, 1797
BstMAI GTCTC 6 cut(s) 129, 137, 264, 980, 1002, 1967
BstMWI GCNNNNNNNGC 3 cut(s) 107, 878, 2411
BstNI CCWGG 5 cut(s) 483, 501, 1370, 1937, 2349
BstNSI RCATGY 7 cut(s) 727, 885, 1015, 2008, 2150, 2293, 2409
BstPI GGTNACC 1 cut(s) 307
BstSCI CCNGG 7 cut(s) 316, 481, 499, 1155, 1368, 1935, 2347
BstSFI CTRYAG 5 cut(s) 147, 715, 1479, 2056, 2298
BstV1I GCAGC 7 cut(s) 57, 95, 793, 1265, 1417, 1673, 2275
BstV2I GAAGAC 2 cut(s) 1278, 1704
BstX2I RGATCY 4 cut(s) 571, 1447, 1873, 2368
BstXI CCANNNNNNTGG 1 cut(s) 1418
BstYI RGATCY 4 cut(s) 571, 1447, 1873, 2368
Bsu15I ATCGAT 2 cut(s) 1287, 2025
Bsu36I CCTNAGG 1 cut(s) 567
BsuRI GGCC 6 cut(s) 1250, 1374, 1789, 1935, 2176, 2284
BsuTUI ATCGAT 2 cut(s) 1287, 2025
BtrI CACGTC 1 cut(s) 398
BtsCI GGATG 6 cut(s) 327, 1165, 1688, 1779, 2116, 2160
BtsI GCAGTG 2 cut(s) 1584, 1909
BtsIMutI CAGTG 5 cut(s) 396, 649, 1584, 1909, 2365
Cac8I GCNNGC 2 cut(s) 2174, 2407
CfoI GCGC 2 cut(s) 786, 1797
Cfr10I RCCGGY 1 cut(s) 2387
Cfr13I GGNCC 3 cut(s) 357, 605, 1372
ClaI ATCGAT 2 cut(s) 1287, 2025
CseI GACGC 2 cut(s) 116, 1541
CsiI ACCWGGT 2 cut(s) 499, 2347
Csp6I GTAC 9 cut(s) 117, 165, 226, 712, 1024, 1174, 1186, 1551, 2435
CviQI GTAC 9 cut(s) 117, 165, 226, 712, 1024, 1174, 1186, 1551, 2435
DdeI CTNAG 8 cut(s) 36, 450, 548, 567, 654, 696, 1741, 1924
EaeI YGGCCR 2 cut(s) 1248, 1787
Eam1104I CTCTTC 2 cut(s) 67, 1085
EarI CTCTTC 2 cut(s) 67, 1085
Eco130I CCWWGG 1 cut(s) 609
Eco147I AGGCCT 1 cut(s) 2176
Eco24I GRGCYC 1 cut(s) 1830
Eco32I GATATC 2 cut(s) 237, 1153
Eco47I GGWCC 2 cut(s) 357, 605
Eco47III AGCGCT 2 cut(s) 785, 1796
Eco57I CTGAAG 3 cut(s) 15, 750, 1637
Eco81I CCTNAGG 1 cut(s) 567
Eco88I CYCGRG 2 cut(s) 1027, 1821
Eco91I GGTNACC 1 cut(s) 307
EcoO65I GGTNACC 1 cut(s) 307
EcoRI GAATTC 1 cut(s) 321
EcoRII CCWGG 5 cut(s) 481, 499, 1368, 1935, 2347
EcoRV GATATC 2 cut(s) 237, 1153
EcoT14I CCWWGG 1 cut(s) 609
EcoT22I ATGCAT 2 cut(s) 862, 2152
EcoT38I GRGCYC 1 cut(s) 1830
ErhI CCWWGG 1 cut(s) 609
Esp3I CGTCTC 2 cut(s) 129, 1967
FalI AAGNNNNNCTT 2 cut(s) 29, 61
FaqI GGGAC 3 cut(s) 591, 1443, 1993
FblI GTMKAC 1 cut(s) 2055
Fnu4HI GCNGC 8 cut(s) 71, 84, 782, 1251, 1254, 1406, 1687, 2264
FokI GGATG 6 cut(s) 314, 1172, 1695, 1786, 2103, 2167
FriOI GRGCYC 1 cut(s) 1830
Fsp4HI GCNGC 8 cut(s) 71, 84, 782, 1251, 1254, 1406, 1687, 2264
FspBI CTAG 9 cut(s) 114, 176, 1044, 1262, 1602, 1667, 1851, 1941, 2078
GlaI GCGC 2 cut(s) 785, 1796
GluI GCNGC 8 cut(s) 71, 84, 782, 1251, 1254, 1406, 1687, 2264
GsuI CTGGAG 1 cut(s) 978
HaeII RGCGCY 2 cut(s) 787, 1798
HaeIII GGCC 6 cut(s) 1250, 1374, 1789, 1935, 2176, 2284
HapII CCGG 6 cut(s) 318, 637, 1005, 1156, 1356, 2388
HgaI GACGC 2 cut(s) 116, 1541
HhaI GCGC 2 cut(s) 786, 1797
Hin1I GRCGYC 2 cut(s) 108, 2052
Hin6I GCGC 2 cut(s) 784, 1795
HinP1I GCGC 2 cut(s) 784, 1795
HincII GTYRAC 1 cut(s) 762
HindII GTYRAC 1 cut(s) 762
HindIII AAGCTT 3 cut(s) 27, 1673, 1945
HpaI GTTAAC 1 cut(s) 762
HpaII CCGG 6 cut(s) 318, 637, 1005, 1156, 1356, 2388
HphI GGTGA 4 cut(s) 152, 301, 1535, 2330
Hpy166II GTNNAC 2 cut(s) 762, 2056
Hpy188I TCNGA 8 cut(s) 697, 769, 1085, 1197, 1219, 1303, 1927, 2050
Hpy8I GTNNAC 2 cut(s) 762, 2056
Hpy99I CGWCG 4 cut(s) 25, 1535, 1619, 2054
HpyAV CCTTC 5 cut(s) 298, 1520, 1764, 2147, 2315
HpyCH4IV ACGT 3 cut(s) 167, 397, 2052
HpyF10VI GCNNNNNNNGC 3 cut(s) 107, 878, 2411
HpyF3I CTNAG 8 cut(s) 36, 450, 548, 567, 654, 696, 1741, 1924
HpySE526I ACGT 3 cut(s) 167, 397, 2052
Hsp92I GRCGYC 2 cut(s) 108, 2052
HspAI GCGC 2 cut(s) 784, 1795
KspAI GTTAAC 1 cut(s) 762
LguI GCTCTTC 1 cut(s) 1085
LmnI GCTCC 3 cut(s) 1130, 1833, 2094
Lsp1109I GCAGC 7 cut(s) 57, 95, 793, 1265, 1417, 1673, 2275
LweI GCATC 5 cut(s) 184, 1267, 1279, 1673, 2145
MabI ACCWGGT 2 cut(s) 499, 2347
MaeI CTAG 9 cut(s) 114, 176, 1044, 1262, 1602, 1667, 1851, 1941, 2078
MaeII ACGT 3 cut(s) 167, 397, 2052
MfeI CAATTG 2 cut(s) 538, 1506
MflI RGATCY 4 cut(s) 571, 1447, 1873, 2368
MhlI GDGCHC 3 cut(s) 350, 1080, 1830
MlyI GAGTC 2 cut(s) 1605, 2201
MmeI TCCRAC 4 cut(s) 387, 1399, 1969, 2073
Mph1103I ATGCAT 2 cut(s) 862, 2152
MseI TTAA 5 cut(s) 659, 761, 1065, 1809, 1911
MslI CAYNNNNRTG 1 cut(s) 1343
MspA1I CMGCKG 2 cut(s) 101, 2266
MspI CCGG 6 cut(s) 318, 637, 1005, 1156, 1356, 2388
MspR9I CCNGG 7 cut(s) 318, 483, 501, 1157, 1370, 1937, 2349
MunI CAATTG 2 cut(s) 538, 1506
Mva1269I GAATGC 2 cut(s) 860, 1689
MvaI CCWGG 5 cut(s) 483, 501, 1370, 1937, 2349
MwoI GCNNNNNNNGC 3 cut(s) 107, 878, 2411
NciI CCSGG 2 cut(s) 318, 1157
NlaIV GGNNCC 3 cut(s) 573, 607, 1829
NmeAIII GCCGAG 1 cut(s) 2390
NmuCI GTSAC 3 cut(s) 307, 848, 2422
NsiI ATGCAT 2 cut(s) 862, 2152
NspI RCATGY 7 cut(s) 727, 885, 1015, 2008, 2150, 2293, 2409
OliI CACNNNNGTG 1 cut(s) 1343
PaeI GCATGC 1 cut(s) 2409
PaeR7I CTCGAG 1 cut(s) 1821
PceI AGGCCT 1 cut(s) 2176
PciI ACATGT 2 cut(s) 1011, 2004
PciSI GCTCTTC 1 cut(s) 1085
PctI GAATGC 2 cut(s) 860, 1689
PfeI GAWTC 8 cut(s) 385, 407, 1304, 1326, 1847, 2109, 2192, 2269
PflMI CCANNNNNTGG 3 cut(s) 255, 813, 948
PfoI TCCNGGA 1 cut(s) 1155
PkrI GCNGC 8 cut(s) 72, 85, 783, 1252, 1255, 1407, 1688, 2265
PleI GAGTC 2 cut(s) 1605, 2201
PpsI GAGTC 2 cut(s) 1605, 2201
PscI ACATGT 2 cut(s) 1011, 2004
PshBI ATTAAT 1 cut(s) 1809
Psp6I CCWGG 5 cut(s) 481, 499, 1368, 1935, 2347
PspEI GGTNACC 1 cut(s) 307
PspGI CCWGG 5 cut(s) 481, 499, 1368, 1935, 2347
PspN4I GGNNCC 3 cut(s) 573, 607, 1829
PspPI GGNCC 3 cut(s) 357, 605, 1372
PspXI VCTCGAGB 1 cut(s) 1821
PsuI RGATCY 4 cut(s) 571, 1447, 1873, 2368
PvuII CAGCTG 1 cut(s) 101
RsaI GTAC 9 cut(s) 118, 166, 227, 713, 1025, 1175, 1187, 1552, 2436
RsaNI GTAC 9 cut(s) 117, 165, 226, 712, 1024, 1174, 1186, 1551, 2435
RseI CAYNNNNRTG 1 cut(s) 1343
SapI GCTCTTC 1 cut(s) 1085
SaqAI TTAA 5 cut(s) 659, 761, 1065, 1809, 1911
SatI GCNGC 8 cut(s) 71, 84, 782, 1251, 1254, 1406, 1687, 2264
Sau96I GGNCC 3 cut(s) 357, 605, 1372
ScaI AGTACT 2 cut(s) 1025, 2436
SchI GAGTC 2 cut(s) 1605, 2201
ScrFI CCNGG 7 cut(s) 318, 483, 501, 1157, 1370, 1937, 2349
SduI GDGCHC 3 cut(s) 350, 1080, 1830
SexAI ACCWGGT 2 cut(s) 499, 2347
SfaNI GCATC 5 cut(s) 184, 1267, 1279, 1673, 2145
SfcI CTRYAG 5 cut(s) 147, 715, 1479, 2056, 2298
Sfr274I CTCGAG 1 cut(s) 1821
SinI GGWCC 2 cut(s) 357, 605
SlaI CTCGAG 1 cut(s) 1821
SmiMI CAYNNNNRTG 1 cut(s) 1343
SmlI CTYRAG 4 cut(s) 579, 1091, 1821, 2438
SmoI CTYRAG 4 cut(s) 579, 1091, 1821, 2438
SpeI ACTAGT 1 cut(s) 113
SphI GCATGC 1 cut(s) 2409
SseBI AGGCCT 1 cut(s) 2176
SsiI CCGC 3 cut(s) 162, 1251, 2266
SspI AATATT 1 cut(s) 627
SspMI CTAG 9 cut(s) 114, 176, 1044, 1262, 1602, 1667, 1851, 1941, 2078
StuI AGGCCT 1 cut(s) 2176
StyD4I CCNGG 7 cut(s) 316, 481, 499, 1155, 1368, 1935, 2347
StyI CCWWGG 1 cut(s) 609
TaiI ACGT 3 cut(s) 170, 400, 2055
TatI WGTACW 4 cut(s) 116, 711, 1023, 2434
TauI GCSGC 1 cut(s) 1253
TfiI GAWTC 8 cut(s) 385, 407, 1304, 1326, 1847, 2109, 2192, 2269
Tru1I TTAA 5 cut(s) 659, 761, 1065, 1809, 1911
Tru9I TTAA 5 cut(s) 659, 761, 1065, 1809, 1911
TscAI CASTG 5 cut(s) 396, 649, 1584, 1909, 2365
TseFI GTSAC 3 cut(s) 307, 848, 2422
TseI GCWGC 7 cut(s) 70, 83, 781, 1253, 1405, 1686, 2263
Tsp45I GTSAC 3 cut(s) 307, 848, 2422
TspGWI ACGGA 3 cut(s) 9, 571, 1457
TspRI CASTG 5 cut(s) 396, 649, 1584, 1909, 2365
Van91I CCANNNNNTGG 3 cut(s) 255, 813, 948
VpaK11BI GGWCC 2 cut(s) 357, 605
VspI ATTAAT 1 cut(s) 1809
XapI RAATTY 3 cut(s) 321, 1144, 1907
XbaI TCTAGA 2 cut(s) 1601, 1850
XceI RCATGY 7 cut(s) 727, 885, 1015, 2008, 2150, 2293, 2409
XhoI CTCGAG 1 cut(s) 1821
XmiI GTMKAC 1 cut(s) 2055
XspI CTAG 9 cut(s) 114, 176, 1044, 1262, 1602, 1667, 1851, 1941, 2078
ZraI GACGTC 1 cut(s) 2053
ZrmI AGTACT 2 cut(s) 1025, 2436
Zsp2I ATGCAT 2 cut(s) 862, 2152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.