Rh5DG184400

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000484
Physical Location & Seq
Forward (+)
20415229 .. 20419225
3997 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG184400.1

Sequence Viewer

Length: 1929 bp
ATGTTGATTGGGAAAGGATTACGCGTTCCTAATTGCCATCTGAAGTTGAATGGCATTGGAACGATATTTTCCACAGCGGGAGGCAGCACAAGTACATTTCAGTCCATTAGAGATGGCCAGACTATAGTTTCAACTACTGGAAACTTTGAGCTTGGATTTTTTAGTCCTCCTACTGGTTCTAATAATCGATATGTCGGGATATGGTACAAGAAGATATCGTCTACAATAGTATGGGTTGCCAACAGAGAAGCACCCCTTACTGATTCATCAGGTGTTCTGCAGGTCACCAACCCTGGAATTCTTGTCCTCCTCAACTATACCAAGAGCATAGTTTGGTCCTCAAACACATCAAGATCTGCAGAGAATCCAGTGGCCCAGCTTTTGGATTCAGGAAATCTTGTTGTAACAGATGGCAGTGATACAGACCCTGAGAACTTCCTGTGGCAAAGTTTTGATTACCCAAGTGATACACTCCTACCAGGTATGAAGCTTGGTTGGAACAAAGTTACAGGCTTCAGTAGGCGTCTTACATCATGGAATAGTCCACAGGATCCTTCTCTAGGAAAATATACATTTGAACTTGGTCGCAAAGGATATGGAGAAGAAATTTTGAAGATGGAGTCAGTTATGATATGGCGAACTGGACCATGGAATGGAATCCGATTCAGTGGCTTGCCTCATTTAAGTCCAAACTCCATATTCACATACAATCTTGTTTTAGATGATCATGGTGAAGATTATTATTTTTTTGAGCTTCGTAACAGCTCACTTTATTCAAGATTGACTCTCAATCCAGATGGGCGTGTGCAGCGCTACACATGGATTGATACAAACCAAACTTGGGGATTCCTTTATCAAACAGCTTTAATTGATAACTGCGAAAGTTATGCATTCTGTGGTCCAAATGGTGCATGCTGTATTGACAGTTTCCCAATATGTCGCTGCTTGAATGGATTTCAACCCAAATATCCAAAGGACTGGGATGTAGTGGATTGGTCAAATGGCTGTGTGAGAAGGACTCCACTAAATTGCAGCGGTGACTTGTTTGAGAAGTACTCTGCAGTGAAATTGCCAAACACAGAGCAGTCCTGGTTCAACAAAAGTATGAATCTCAAGGAATGTCAGATGGCGTGCCTGAAGGACTGTTCCTGCACAGCTTATGCAAATTTGGATATCACGGATGGAGGATCTGGCTGCTTGCTGTGGTTTGGCAACCTAATTGATATTAGAAATTTAACTGCAAATGGGCAAGATATTTATATAAGAATGGCTGCATCAGAACAAGTACGCCTGGATCATGACAATACAGTTATCAATGCTAAAGACTCTGAATCTAAGAAGGTTAGAATCATACTATGTGGTACTCTGTTGCCTACTGGACTGCTGTTCGTGGGCATAGCCCTGGTATTTTATTGCCGGAAGAAGCAGAGCAAAAAATATGGGAGAATGAGGAGCGGCAAAAAAGAAGGTTCAGAACTTACCTCAGGAGAAGAAGGTTTAGAACTTCCCTCCAAAAAGGAAGATTTTGAACTTCCATTATTTGACTTGGCTACTGTCATTTGTGCCACTGATAACTTCTCAGAGAACAGAAAACTTGGACAAGGTGGATTCGGATCCGTCTTTAAGGCATGGAACTTACACACAGAAGGCAGGTCCATTGAATTGCTTGCTACATCAGTAGGGGACTCCATTAATCTACAAGAAGTTCTGCGAACGATTCATGTGGGTCTTTTATGTGTTCAGCAAAATCCACAAGACAGGCCAAGCATGTCAGCTGCAGTACTAAAGTTAAGTGGCGAAGGTGTGCTGCCTGTACCTCAGAAACCTGGTTTTTATAATGAGAGGGCTCTAACTGAACTCCAAGTCCATTCTTCTTCACAATCATGTTCAGTTAATACACTCACTTCTAGCCTATTCGTGCCTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

642

Amino Acids

71.27

Weight (kDa)

6.67

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 75 - 179 2e-40 D-mannose binding lectin
S_locus_glycop PF00954 212 - 322 6e-28 S-locus glycoprotein domain
PAN_2 PF08276 344 - 409 1.3e-20 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1836
Acc36I ACCTGC 2 cut(s) 271, 1641
AccB7I CCANNNNNTGG 2 cut(s) 382, 653
AccBSI CCGCTC 1 cut(s) 1455
AccI GTMKAC 1 cut(s) 221
AccII CGCG 1 cut(s) 24
AciI CCGC 3 cut(s) 77, 1035, 1455
AclWI GGATC 6 cut(s) 545, 558, 1195, 1302, 1608, 1621
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 4 cut(s) 297, 606, 1165, 1231
AcuI CTGAAG 3 cut(s) 62, 499, 1157
AcyI GRCGYC 1 cut(s) 523
AfaI GTAC 7 cut(s) 94, 206, 1055, 1287, 1363, 1782, 1815
AfeI AGCGCT 1 cut(s) 812
AfiI CCNNNNNNNGG 5 cut(s) 173, 382, 560, 653, 841
AflIII ACRYGT 1 cut(s) 22
AjnI CCWGG 6 cut(s) 292, 478, 1088, 1290, 1401, 1825
AloI GAACNNNNNNTCC 4 cut(s) 9, 41, 1848, 1880
AluBI AGCT 8 cut(s) 151, 379, 490, 754, 765, 863, 1157, 1775
AluI AGCT 8 cut(s) 151, 379, 490, 754, 765, 863, 1157, 1775
AlwI GGATC 6 cut(s) 545, 558, 1195, 1302, 1608, 1621
AlwNI CAGNNNCTG 1 cut(s) 428
Aor51HI AGCGCT 1 cut(s) 812
AoxI GGCC 3 cut(s) 115, 372, 1760
ApeKI GCWGC 8 cut(s) 84, 808, 942, 1032, 1194, 1271, 1775, 1807
ApoI RAATTY 4 cut(s) 297, 606, 1165, 1231
AseI ATTAAT 1 cut(s) 1692
AspLEI GCGC 1 cut(s) 813
AspS9I GGNCC 5 cut(s) 336, 373, 644, 899, 1653
AsuHPI GGTGA 3 cut(s) 277, 743, 1049
AvaII GGWCC 4 cut(s) 336, 644, 899, 1653
AxyI CCTNAGG 1 cut(s) 1483
BalI TGGCCA 1 cut(s) 117
BamHI GGATCC 2 cut(s) 550, 1613
BanII GRGCYC 1 cut(s) 1849
BbvI GCAGC 8 cut(s) 96, 820, 929, 1044, 1181, 1258, 1762, 1794
BccI CCATC 7 cut(s) 45, 107, 404, 610, 791, 1120, 1175
BcgI CGANNNNNNTGC 2 cut(s) 869, 903
BciT130I CCWGG 6 cut(s) 294, 480, 1090, 1292, 1403, 1827
BclI TGATCA 1 cut(s) 724
BfaI CTAG 2 cut(s) 560, 1908
BfmI CTRYAG 5 cut(s) 123, 278, 357, 1059, 1776
BfoI RGCGCY 1 cut(s) 814
BfuAI ACCTGC 2 cut(s) 271, 1641
BglII AGATCT 1 cut(s) 353
BisI GCNGC 9 cut(s) 85, 809, 943, 1033, 1195, 1272, 1456, 1776, 1808
BlsI GCNGC 9 cut(s) 86, 810, 944, 1034, 1196, 1273, 1457, 1777, 1809
BmcAI AGTACT 2 cut(s) 1055, 1782
Bme1390I CCNGG 6 cut(s) 294, 480, 1090, 1292, 1403, 1827
Bme18I GGWCC 4 cut(s) 336, 644, 899, 1653
BmgT120I GGNCC 5 cut(s) 336, 373, 644, 899, 1653
BmiI GGNNCC 2 cut(s) 552, 1615
BmrFI CCNGG 6 cut(s) 294, 480, 1090, 1292, 1403, 1827
BmrI ACTGGG 1 cut(s) 988
BmsI GCATC 1 cut(s) 1283
BmuI ACTGGG 1 cut(s) 988
BplI GAGNNNNNCTC 4 cut(s) 1003, 1035, 1040, 1072
BpuEI CTTGAG 1 cut(s) 1097
Bsa29I ATCGAT 1 cut(s) 187
BsaBI GATNNNNATC 1 cut(s) 1612
BsaHI GRCGYC 1 cut(s) 523
BsaJI CCNNGG 3 cut(s) 292, 647, 1401
Bsc4I CCNNNNNNNGG 5 cut(s) 173, 382, 560, 653, 841
Bse1I ACTGG 6 cut(s) 142, 178, 368, 646, 983, 1381
Bse21I CCTNAGG 1 cut(s) 1483
Bse8I GATNNNNATC 1 cut(s) 1612
BseBI CCWGG 6 cut(s) 294, 480, 1090, 1292, 1403, 1827
BseCI ATCGAT 1 cut(s) 187
BseDI CCNNGG 3 cut(s) 292, 647, 1401
BseGI GGATG 2 cut(s) 988, 1186
BseJI GATNNNNATC 1 cut(s) 1612
BseLI CCNNNNNNNGG 5 cut(s) 173, 382, 560, 653, 841
BseMII CTCAG 4 cut(s) 420, 1497, 1593, 1832
BseNI ACTGG 6 cut(s) 142, 178, 368, 646, 983, 1381
BseRI GAGGAG 2 cut(s) 299, 1465
BseXI GCAGC 8 cut(s) 96, 820, 929, 1044, 1181, 1258, 1762, 1794
BseYI CCCAGC 1 cut(s) 375
BsgI GTGCAG 2 cut(s) 827, 1135
Bsh1236I CGCG 1 cut(s) 24
BshFI GGCC 3 cut(s) 117, 374, 1762
BshVI ATCGAT 1 cut(s) 187
BsiSI CCGG 1 cut(s) 1417
BslFI GGGAC 1 cut(s) 1697
BslI CCNNNNNNNGG 5 cut(s) 173, 382, 560, 653, 841
BsmFI GGGAC 1 cut(s) 1697
BsmI GAATGC 1 cut(s) 890
BsnI GGCC 3 cut(s) 117, 374, 1762
Bsp1286I GDGCHC 1 cut(s) 1849
Bsp143I GATC 6 cut(s) 353, 550, 724, 1187, 1294, 1613
Bsp19I CCATGG 1 cut(s) 647
BspACI CCGC 3 cut(s) 77, 1035, 1455
BspANI GGCC 3 cut(s) 117, 374, 1762
BspCNI CTCAG 4 cut(s) 421, 1496, 1592, 1831
BspDI ATCGAT 1 cut(s) 187
BspFNI CGCG 1 cut(s) 24
BspHI TCATGA 1 cut(s) 1297
BspLI GGNNCC 2 cut(s) 552, 1615
BspMAI CTGCAG 4 cut(s) 282, 361, 1063, 1780
BspMI ACCTGC 2 cut(s) 271, 1641
BspPI GGATC 6 cut(s) 545, 558, 1195, 1302, 1608, 1621
BsrBI CCGCTC 1 cut(s) 1455
BsrI ACTGG 6 cut(s) 142, 178, 368, 646, 983, 1381
BssECI CCNNGG 3 cut(s) 292, 647, 1401
BssMI GATC 6 cut(s) 353, 550, 724, 1187, 1294, 1613
BssNI GRCGYC 1 cut(s) 523
BssT1I CCWWGG 1 cut(s) 647
Bst2UI CCWGG 6 cut(s) 294, 480, 1090, 1292, 1403, 1827
Bst4CI ACNGT 4 cut(s) 926, 1145, 1309, 1555
BstACI GRCGYC 1 cut(s) 523
BstC8I GCNNGC 5 cut(s) 674, 913, 1132, 1199, 1668
BstDEI CTNAG 5 cut(s) 429, 1335, 1483, 1579, 1818
BstDSI CCRYGG 1 cut(s) 647
BstEII GGTNACC 1 cut(s) 283
BstENI CCTNNNNNAGG 1 cut(s) 558
BstF5I GGATG 2 cut(s) 988, 1186
BstFNI CGCG 1 cut(s) 24
BstH2I RGCGCY 1 cut(s) 814
BstHHI GCGC 1 cut(s) 813
BstKTI GATC 6 cut(s) 356, 553, 727, 1190, 1297, 1616
BstMBI GATC 6 cut(s) 353, 550, 724, 1187, 1294, 1613
BstMWI GCNNNNNNNGC 1 cut(s) 808
BstNI CCWGG 6 cut(s) 294, 480, 1090, 1292, 1403, 1827
BstNSI RCATGY 2 cut(s) 915, 1771
BstPI GGTNACC 1 cut(s) 283
BstSCI CCNGG 6 cut(s) 292, 478, 1088, 1290, 1401, 1825
BstSFI CTRYAG 5 cut(s) 123, 278, 357, 1059, 1776
BstUI CGCG 1 cut(s) 24
BstV1I GCAGC 8 cut(s) 96, 820, 929, 1044, 1181, 1258, 1762, 1794
BstX2I RGATCY 4 cut(s) 353, 550, 1187, 1613
BstXI CCANNNNNNTGG 1 cut(s) 978
BstYI RGATCY 4 cut(s) 353, 550, 1187, 1613
Bsu15I ATCGAT 1 cut(s) 187
Bsu36I CCTNAGG 1 cut(s) 1483
BsuRI GGCC 3 cut(s) 117, 374, 1762
BsuTUI ATCGAT 1 cut(s) 187
BtgI CCRYGG 1 cut(s) 647
BtsCI GGATG 2 cut(s) 988, 1186
BtsI GCAGTG 2 cut(s) 421, 1068
BtsIMutI CAGTG 5 cut(s) 375, 421, 673, 1068, 1566
BveI ACCTGC 2 cut(s) 271, 1641
Cac8I GCNNGC 5 cut(s) 674, 913, 1132, 1199, 1668
CaiI CAGNNNCTG 1 cut(s) 428
CciI TCATGA 1 cut(s) 1297
CfoI GCGC 1 cut(s) 813
Cfr13I GGNCC 5 cut(s) 336, 373, 644, 899, 1653
ClaI ATCGAT 1 cut(s) 187
CseI GACGC 1 cut(s) 512
CsiI ACCWGGT 2 cut(s) 478, 1825
Csp6I GTAC 7 cut(s) 93, 205, 1054, 1286, 1362, 1781, 1814
CviQI GTAC 7 cut(s) 93, 205, 1054, 1286, 1362, 1781, 1814
DdeI CTNAG 5 cut(s) 429, 1335, 1483, 1579, 1818
DpnI GATC 6 cut(s) 355, 552, 726, 1189, 1296, 1615
DpnII GATC 6 cut(s) 353, 550, 724, 1187, 1294, 1613
EaeI YGGCCR 1 cut(s) 115
Eco130I CCWWGG 1 cut(s) 647
Eco24I GRGCYC 1 cut(s) 1849
Eco32I GATATC 2 cut(s) 216, 1174
Eco47I GGWCC 4 cut(s) 336, 644, 899, 1653
Eco47III AGCGCT 1 cut(s) 812
Eco57I CTGAAG 3 cut(s) 62, 499, 1157
Eco81I CCTNAGG 1 cut(s) 1483
Eco91I GGTNACC 1 cut(s) 283
EcoNI CCTNNNNNAGG 1 cut(s) 558
EcoO65I GGTNACC 1 cut(s) 283
EcoRI GAATTC 1 cut(s) 297
EcoRII CCWGG 6 cut(s) 292, 478, 1088, 1290, 1401, 1825
EcoRV GATATC 2 cut(s) 216, 1174
EcoT14I CCWWGG 1 cut(s) 647
EcoT22I ATGCAT 1 cut(s) 892
EcoT38I GRGCYC 1 cut(s) 1849
ErhI CCWWGG 1 cut(s) 647
FalI AAGNNNNNCTT 2 cut(s) 240, 272
FaqI GGGAC 1 cut(s) 1697
FauI CCCGC 1 cut(s) 70
FbaI TGATCA 1 cut(s) 724
FblI GTMKAC 1 cut(s) 221
Fnu4HI GCNGC 9 cut(s) 85, 809, 943, 1033, 1195, 1272, 1456, 1776, 1808
FokI GGATG 2 cut(s) 995, 1193
FriOI GRGCYC 1 cut(s) 1849
Fsp4HI GCNGC 9 cut(s) 85, 809, 943, 1033, 1195, 1272, 1456, 1776, 1808
FspBI CTAG 2 cut(s) 560, 1908
GlaI GCGC 1 cut(s) 812
GluI GCNGC 9 cut(s) 85, 809, 943, 1033, 1195, 1272, 1456, 1776, 1808
GsaI CCCAGC 1 cut(s) 379
HaeII RGCGCY 1 cut(s) 814
HaeIII GGCC 3 cut(s) 117, 374, 1762
HapII CCGG 1 cut(s) 1417
HgaI GACGC 1 cut(s) 512
HhaI GCGC 1 cut(s) 813
Hin1I GRCGYC 1 cut(s) 523
Hin6I GCGC 1 cut(s) 811
HinP1I GCGC 1 cut(s) 811
HindIII AAGCTT 1 cut(s) 488
HpaII CCGG 1 cut(s) 1417
HphI GGTGA 3 cut(s) 277, 743, 1049
Hpy166II GTNNAC 2 cut(s) 222, 545
Hpy188I TCNGA 9 cut(s) 42, 662, 1125, 1279, 1330, 1474, 1582, 1613, 1821
Hpy188III TCNNGA 7 cut(s) 196, 351, 390, 777, 794, 1298, 1485
Hpy8I GTNNAC 2 cut(s) 222, 545
HpyAV CCTTC 8 cut(s) 564, 1008, 1132, 1333, 1460, 1487, 1640, 1793
HpyCH4III ACNGT 4 cut(s) 926, 1145, 1309, 1555
HpyF10VI GCNNNNNNNGC 1 cut(s) 808
HpyF3I CTNAG 5 cut(s) 429, 1335, 1483, 1579, 1818
Hsp92I GRCGYC 1 cut(s) 523
HspAI GCGC 1 cut(s) 811
Ksp22I TGATCA 1 cut(s) 724
Kzo9I GATC 6 cut(s) 353, 550, 724, 1187, 1294, 1613
LmnI GCTCC 1 cut(s) 1452
Lsp1109I GCAGC 8 cut(s) 96, 820, 929, 1044, 1181, 1258, 1762, 1794
LweI GCATC 1 cut(s) 1283
MabI ACCWGGT 2 cut(s) 478, 1825
MaeI CTAG 2 cut(s) 560, 1908
MaeIII GTNAC 5 cut(s) 283, 403, 505, 758, 1037
MalI GATC 6 cut(s) 355, 552, 726, 1189, 1296, 1615
MbiI CCGCTC 1 cut(s) 1455
MboI GATC 6 cut(s) 353, 550, 724, 1187, 1294, 1613
MboII GAAGA 9 cut(s) 223, 614, 625, 746, 1432, 1502, 1532, 1863, 1866
MflI RGATCY 4 cut(s) 353, 550, 1187, 1613
MhlI GDGCHC 1 cut(s) 1849
MlsI TGGCCA 1 cut(s) 117
MluI ACGCGT 1 cut(s) 22
MluNI TGGCCA 1 cut(s) 117
MlyI GAGTC 5 cut(s) 629, 778, 1012, 1319, 1679
MmeI TCCRAC 1 cut(s) 476
Mox20I TGGCCA 1 cut(s) 117
Mph1103I ATGCAT 1 cut(s) 892
MscI TGGCCA 1 cut(s) 117
MseI TTAA 8 cut(s) 683, 866, 1235, 1623, 1692, 1790, 1893, 1927
MslI CAYNNNNRTG 1 cut(s) 1882
Msp20I TGGCCA 1 cut(s) 117
MspA1I CMGCKG 3 cut(s) 77, 1035, 1775
MspI CCGG 1 cut(s) 1417
MspR9I CCNGG 6 cut(s) 294, 480, 1090, 1292, 1403, 1827
Mva1269I GAATGC 1 cut(s) 890
MvaI CCWGG 6 cut(s) 294, 480, 1090, 1292, 1403, 1827
MvnI CGCG 1 cut(s) 24
MwoI GCNNNNNNNGC 1 cut(s) 808
NcoI CCATGG 1 cut(s) 647
NdeII GATC 6 cut(s) 353, 550, 724, 1187, 1294, 1613
NlaIV GGNNCC 2 cut(s) 552, 1615
NmuCI GTSAC 2 cut(s) 283, 1037
NsiI ATGCAT 1 cut(s) 892
NspI RCATGY 2 cut(s) 915, 1771
PaeI GCATGC 1 cut(s) 915
PagI TCATGA 1 cut(s) 1297
PctI GAATGC 1 cut(s) 890
PflMI CCANNNNNTGG 2 cut(s) 382, 653
PkrI GCNGC 9 cut(s) 86, 810, 944, 1034, 1196, 1273, 1457, 1777, 1809
PleI GAGTC 5 cut(s) 628, 778, 1012, 1319, 1679
PpsI GAGTC 5 cut(s) 628, 778, 1012, 1319, 1679
PshBI ATTAAT 1 cut(s) 1692
PsiI TTATAA 1 cut(s) 1836
Psp6I CCWGG 6 cut(s) 292, 478, 1088, 1290, 1401, 1825
PspEI GGTNACC 1 cut(s) 283
PspFI CCCAGC 1 cut(s) 375
PspGI CCWGG 6 cut(s) 292, 478, 1088, 1290, 1401, 1825
PspN4I GGNNCC 2 cut(s) 552, 1615
PspPI GGNCC 5 cut(s) 336, 373, 644, 899, 1653
PstI CTGCAG 4 cut(s) 282, 361, 1063, 1780
PstNI CAGNNNCTG 1 cut(s) 428
PsuI RGATCY 4 cut(s) 353, 550, 1187, 1613
PvuII CAGCTG 1 cut(s) 1775
RsaI GTAC 7 cut(s) 94, 206, 1055, 1287, 1363, 1782, 1815
RsaNI GTAC 7 cut(s) 93, 205, 1054, 1286, 1362, 1781, 1814
RseI CAYNNNNRTG 1 cut(s) 1882
SaqAI TTAA 8 cut(s) 683, 866, 1235, 1623, 1692, 1790, 1893, 1927
SatI GCNGC 9 cut(s) 85, 809, 943, 1033, 1195, 1272, 1456, 1776, 1808
Sau3AI GATC 6 cut(s) 353, 550, 724, 1187, 1294, 1613
Sau96I GGNCC 5 cut(s) 336, 373, 644, 899, 1653
ScaI AGTACT 2 cut(s) 1055, 1782
SchI GAGTC 5 cut(s) 629, 778, 1012, 1319, 1679
ScrFI CCNGG 6 cut(s) 294, 480, 1090, 1292, 1403, 1827
SduI GDGCHC 1 cut(s) 1849
SexAI ACCWGGT 2 cut(s) 478, 1825
SfaNI GCATC 1 cut(s) 1283
SfcI CTRYAG 5 cut(s) 123, 278, 357, 1059, 1776
SinI GGWCC 4 cut(s) 336, 644, 899, 1653
SmiMI CAYNNNNRTG 1 cut(s) 1882
SmlI CTYRAG 1 cut(s) 1112
SmoI CTYRAG 1 cut(s) 1112
SphI GCATGC 1 cut(s) 915
SsiI CCGC 3 cut(s) 77, 1035, 1455
SspMI CTAG 2 cut(s) 560, 1908
StyD4I CCNGG 6 cut(s) 292, 478, 1088, 1290, 1401, 1825
StyI CCWWGG 1 cut(s) 647
TaaI ACNGT 4 cut(s) 926, 1145, 1309, 1555
TaqI TCGA 1 cut(s) 187
TatI WGTACW 3 cut(s) 92, 1053, 1780
TauI GCSGC 1 cut(s) 1458
Tru1I TTAA 8 cut(s) 683, 866, 1235, 1623, 1692, 1790, 1893, 1927
Tru9I TTAA 8 cut(s) 683, 866, 1235, 1623, 1692, 1790, 1893, 1927
TscAI CASTG 5 cut(s) 375, 421, 673, 1068, 1573
TseFI GTSAC 2 cut(s) 283, 1037
TseI GCWGC 8 cut(s) 84, 808, 942, 1032, 1194, 1271, 1775, 1807
Tsp45I GTSAC 2 cut(s) 283, 1037
TspDTI ATGAA 4 cut(s) 255, 500, 1121, 1709
TspGWI ACGGA 2 cut(s) 1193, 1606
TspRI CASTG 5 cut(s) 375, 421, 673, 1068, 1573
Van91I CCANNNNNTGG 2 cut(s) 382, 653
VpaK11BI GGWCC 4 cut(s) 336, 644, 899, 1653
VspI ATTAAT 1 cut(s) 1692
XagI CCTNNNNNAGG 1 cut(s) 558
XapI RAATTY 4 cut(s) 297, 606, 1165, 1231
XceI RCATGY 2 cut(s) 915, 1771
XmiI GTMKAC 1 cut(s) 221
XspI CTAG 2 cut(s) 560, 1908
ZrmI AGTACT 2 cut(s) 1055, 1782
Zsp2I ATGCAT 1 cut(s) 892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.