Rw5G016850

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000484
Physical Location & Seq
Forward (+)
21495805 .. 21500606
4802 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G016850.1

Sequence Viewer

Length: 2547 bp
ATGACACTAGCTCCATTCCAAGAGGACCATTGTGGTGGGAAAGGATTACGCATTCCTAATTGCCATCCGAAGTTGAATGGCATTGGAATGGGAGGCAGCACAAGTACATTTCAGTCCGTTAGAGATGGCCAGACTATAGTTTCAACTACTGGAAACTTTGAGCTTGGATTTTTTAGTCCTGCTACTGGTTCTAATAACCGATACCTTGGGATATGGTACAAGAAGATATCGGCTACCATAGTGTGGGTTGCCAACAGAGAAGCACCCCTTACTGATTCATCAGGTGTTCTGCAGGTCACCGACCCTGGAATTCTTGTCCTCCTCAACCATAACAAGAGCATAGTTTGGTCCTCAAACACATCAAGATCTGCAGAGAATCCAGTGGCCCAGCTTTTGGATTCAGGAAATCTTGTTGTAACAGACGGCAGTGATACAGACCCTGAGAACTTCTTGTGGCAAAGTTTTGATTACCCAAGTGATACGCTCCTACCAGGTATGAAGCTTGGTTGGAACAAAGTTACTGGCTTCAATAGGCATCTTACATCATGGAACAGTCCACAGGATCCTTCTCTAGGAAAATATACATATGAACTTGATCGCAGAGGATATGGAGAAGAAATTTTGAAGAAGGATTCAGTTATGATATGGCGAACTGGACCATGGAATGGAATCCGATTCAGTGGCACGCCTCATTTAACTCCAAACTCCATATTCACATACAATCTTGTTTTTGATGATCATGATGAAGAATATTATATTTTTGAGCTTCTTAACAGCTCAGTTTATTCAAGAGTGATCCTCACTCCAGATGGGCGTGTGCAGCGCTACACATGGATTGATACAACCCAAACTTGGGGATTTCTTTATCAAACAGCTTTAATTGATAACTGCGACAGTTATGCATTCTGTGGTCCAAATGGTGCATGCAGTATTGACAGTTCCCCAGTATGTCGCTGCTTGAATGGATTTCAACCCAAAAATCCAAAGGACTGGGATGTAGTGGATTGGTCAAATGGCTGTGTGAGAAGGACTCCACTAAATTCAGTAAATTGCAGCGGTGACTTGTTTGAGAAGTACTCTGCAGTGAAATTGCCAAACACAGAGCAGTCCTGGTTCAACAGAAGTATGAATCTGAAGGAATGTGAGATGGTGTGCCTGAAGAACTGTTCCTGCACAGCTTATGCAAATTTGGATATCACGGATGGAGGATCTGGCTGCTTGCTGTGGTTTGGCAACCTAATTGATATTAGAAATTTAACTGTAAATGGGCAAGATATTTATATAAGAATGGCTGCATCAGAACTAGGCCTGGATCATGACAACACAGTTATCAATGCTAAAGACTCTGAATCTAAGAAGGTTAGAATCATACTATGTGGTACTCTGTTGCCTACTGGACTGCTGATCGTGGGCATAGCCCTGGTATTTTATTGCCGGAAGAAGCAGCGCAAAAAATATGGGAAAATGAGGAGCAGCAAAAAAGAAGATTCAGAACTTACCTCAAGAGAAGAAGATTTAGGACTTCCCTCAAAGAAGGAAGATTTTGAACTTCCATTATTTGACTTGGCTACTGTCGTTTGTGCCACCGATAACTTCTCAGAGAGCAGCAAACTTGGAGAAGGTGGATTCGGATCCGTCTTTAAGGGTATGTTGAAAGATGGACAGGAAATAGCTGTGAAGAGGCTCTCGAAACATTCTGCTCAAGGATTGGATGAGTTCAAGAATGAGGTTACACATATTGTCAAACTTCAGCACAGGAATCTAGTGAGGCTTCTAGGATGCTGCATTCAGGAAGATGAAATGATGCTGATCTACGAATGCATGCCTAACAAAAGCTTGGACTTCTTTATTTTTGATCAAAGAAGAAGCTTCTTACTAGATTGGTCTAAGCGCTTTGACATTATTAATGGTATTGCTCGGGGGATCCTTTATCTTCATCAAGATTCTAGACTGAGGGTAATTCATAGAGATCTCAAAGCAGGCAATATCTTATTAGACAGTGAATTCAACCCAAAAATCTCAGACTTCGGCCTGGCTAGAAGTTTTGGAGGAAATGAAACTAAAGCAGAAACGAATAAAGTGGTCGGGACATATGGTTACATGTCCCCAGAATATGCAATTGACGGGTTCTACTCGATCAAATCTGACGTGTATAGCTTTGGTGTTTTGATGCTGGAGATAATAAGTGGGAAGAGAAACAGAGGATTCCTTCATCCAGGCCACAACCTCAACCTGCTTGGACATGCATGGAACTTACACACAGAAGGCAGGTCCATTGAATTGCTTGCTACATCAGTAGGGGACTCCATTAATCTACAAGAAGTTCTGCGAGCGATTCATGTGGGTCTTTTATGTGTTCAGCAAAATCCACAAGACAGGCCAAGCATGTCAGCTGCAGTTCTAATGTTAAGTGGTGAAGGTGTGCTGCCTGTACCTCAGAAACCTGGTTTTTATAATGAGCGGGCTCTAACTGAACTCCAAGTCCATTCTTCTTCACAAGCATGTTCAGTTAATATGCTCACTTCCACCCTATTCGAGCCTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

848

Amino Acids

94.78

Weight (kDa)

6.1

Isoelectric Point (pI)

45.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 79 - 183 7.4e-41 D-mannose binding lectin
S_locus_glycop PF00954 216 - 327 3.5e-29 S-locus glycoprotein domain
PAN_2 PF08276 351 - 416 9.4e-21 PAN-like domain
Pkinase PF00069 533 - 732 2.8e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 535 - 798 2.2e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2454
Acc36I ACCTGC 3 cut(s) 283, 2241, 2259
AccB7I CCANNNNNTGG 3 cut(s) 243, 394, 665
AccBSI CCGCTC 1 cut(s) 2461
AciI CCGC 2 cut(s) 1056, 2461
AclWI GGATC 9 cut(s) 557, 570, 790, 1216, 1320, 1626, 1639, 1918, 1931
AcoI YGGCCR 1 cut(s) 127
AcsI RAATTY 6 cut(s) 309, 618, 1039, 1186, 1252, 2003
AcuI CTGAAG 3 cut(s) 1154, 1178, 1733
AfaI GTAC 5 cut(s) 106, 218, 1076, 1381, 2433
AfeI AGCGCT 2 cut(s) 824, 1892
AfiI CCNNNNNNNGG 7 cut(s) 185, 243, 394, 572, 665, 852, 853
AflIII ACRYGT 2 cut(s) 2100, 2148
AjiI CACGTC 1 cut(s) 2149
AjnI CCWGG 8 cut(s) 304, 490, 1109, 1308, 1419, 2031, 2215, 2443
AloI GAACNNNNNNTCC 2 cut(s) 2466, 2498
AlwI GGATC 9 cut(s) 557, 570, 790, 1216, 1320, 1626, 1639, 1918, 1931
AlwNI CAGNNNCTG 1 cut(s) 440
Ama87I CYCGRG 1 cut(s) 1917
Aor51HI AGCGCT 2 cut(s) 824, 1892
AoxI GGCC 6 cut(s) 127, 384, 1306, 2029, 2218, 2378
ApoI RAATTY 6 cut(s) 309, 618, 1039, 1186, 1252, 2003
AseI ATTAAT 2 cut(s) 1905, 2310
AspLEI GCGC 3 cut(s) 825, 1449, 1893
AspS9I GGNCC 6 cut(s) 25, 348, 385, 656, 911, 2271
AsuHPI GGTGA 3 cut(s) 289, 1070, 2426
AvaI CYCGRG 1 cut(s) 1917
AvaII GGWCC 5 cut(s) 25, 348, 656, 911, 2271
BalI TGGCCA 1 cut(s) 129
BamHI GGATCC 3 cut(s) 562, 1631, 1923
BanII GRGCYC 1 cut(s) 2467
BccI CCATC 6 cut(s) 72, 119, 803, 1141, 1196, 1652
BceAI ACGGC 1 cut(s) 439
BcgI CGANNNNNNTGC 2 cut(s) 881, 915
BciT130I CCWGG 8 cut(s) 306, 492, 1111, 1310, 1421, 2033, 2217, 2445
BclI TGATCA 2 cut(s) 736, 1855
BfaI CTAG 8 cut(s) 8, 572, 1304, 1763, 1775, 1877, 1947, 2037
BfmI CTRYAG 5 cut(s) 135, 290, 369, 1080, 2394
BfoI RGCGCY 2 cut(s) 826, 1894
BfuAI ACCTGC 3 cut(s) 283, 2241, 2259
BglII AGATCT 2 cut(s) 365, 1969
BmcAI AGTACT 1 cut(s) 1076
Bme1390I CCNGG 8 cut(s) 306, 492, 1111, 1310, 1421, 2033, 2217, 2445
Bme18I GGWCC 5 cut(s) 25, 348, 656, 911, 2271
BmeT110I CYCGRG 1 cut(s) 1917
BmgBI CACGTC 1 cut(s) 2149
BmgT120I GGNCC 6 cut(s) 25, 348, 385, 656, 911, 2271
BmiI GGNNCC 3 cut(s) 564, 1633, 1925
BmrFI CCNGG 8 cut(s) 306, 492, 1111, 1310, 1421, 2033, 2217, 2445
BmrI ACTGGG 2 cut(s) 938, 1000
BmsI GCATC 5 cut(s) 544, 1304, 1769, 1794, 2160
BmuI ACTGGG 2 cut(s) 938, 1000
BplI GAGNNNNNCTC 6 cut(s) 783, 815, 1015, 1047, 1061, 1093
BpmI CTGGAG 2 cut(s) 789, 2195
BpuEI CTTGAG 2 cut(s) 1486, 1686
BsaBI GATNNNNATC 2 cut(s) 1630, 1808
BsaJI CCNNGG 4 cut(s) 205, 304, 659, 1419
BsaXI ACNNNNNCTCC 1 cut(s) 25
Bsc4I CCNNNNNNNGG 7 cut(s) 185, 243, 394, 572, 665, 852, 853
Bse1I ACTGG 8 cut(s) 154, 190, 380, 526, 658, 944, 995, 1399
Bse8I GATNNNNATC 2 cut(s) 1630, 1808
BseBI CCWGG 8 cut(s) 306, 492, 1111, 1310, 1421, 2033, 2217, 2445
BseDI CCNNGG 4 cut(s) 205, 304, 659, 1419
BseGI GGATG 6 cut(s) 64, 1000, 1207, 1717, 1784, 2212
BseJI GATNNNNATC 2 cut(s) 1630, 1808
BseLI CCNNNNNNNGG 7 cut(s) 185, 243, 394, 572, 665, 852, 853
BseMII CTCAG 6 cut(s) 432, 792, 1611, 1943, 2034, 2450
BseNI ACTGG 8 cut(s) 154, 190, 380, 526, 658, 944, 995, 1399
BseRI GAGGAG 2 cut(s) 311, 1483
BseYI CCCAGC 1 cut(s) 387
BsgI GTGCAG 2 cut(s) 839, 1156
BshFI GGCC 6 cut(s) 129, 386, 1308, 2031, 2220, 2380
BsiHKCI CYCGRG 1 cut(s) 1917
BsiSI CCGG 1 cut(s) 1435
BslFI GGGAC 3 cut(s) 2089, 2101, 2315
BslI CCNNNNNNNGG 7 cut(s) 185, 243, 394, 572, 665, 852, 853
BsmFI GGGAC 3 cut(s) 2089, 2101, 2315
BsmI GAATGC 4 cut(s) 51, 902, 1785, 1823
BsnI GGCC 6 cut(s) 129, 386, 1308, 2031, 2220, 2380
BsoBI CYCGRG 1 cut(s) 1917
Bsp1286I GDGCHC 1 cut(s) 2467
Bsp19I CCATGG 1 cut(s) 659
BspACI CCGC 2 cut(s) 1056, 2461
BspANI GGCC 6 cut(s) 129, 386, 1308, 2031, 2220, 2380
BspCNI CTCAG 6 cut(s) 433, 791, 1610, 1944, 2033, 2449
BspHI TCATGA 2 cut(s) 739, 1315
BspLI GGNNCC 3 cut(s) 564, 1633, 1925
BspMAI CTGCAG 4 cut(s) 294, 373, 1084, 2398
BspMI ACCTGC 3 cut(s) 283, 2241, 2259
BspPI GGATC 9 cut(s) 557, 570, 790, 1216, 1320, 1626, 1639, 1918, 1931
BsrBI CCGCTC 1 cut(s) 2461
BsrI ACTGG 8 cut(s) 154, 190, 380, 526, 658, 944, 995, 1399
BssECI CCNNGG 4 cut(s) 205, 304, 659, 1419
BssT1I CCWWGG 2 cut(s) 205, 659
Bst2UI CCWGG 8 cut(s) 306, 492, 1111, 1310, 1421, 2033, 2217, 2445
Bst4CI ACNGT 8 cut(s) 554, 896, 938, 1166, 1261, 1327, 1573, 2000
Bst6I CTCTTC 2 cut(s) 1673, 2186
BstC8I GCNNGC 8 cut(s) 686, 925, 1220, 1823, 1981, 2286, 2331, 2463
BstDEI CTNAG 8 cut(s) 441, 778, 1353, 1597, 1887, 1952, 2020, 2436
BstDSI CCRYGG 1 cut(s) 659
BstEII GGTNACC 1 cut(s) 295
BstENI CCTNNNNNAGG 1 cut(s) 570
BstF5I GGATG 6 cut(s) 64, 1000, 1207, 1717, 1784, 2212
BstH2I RGCGCY 2 cut(s) 826, 1894
BstHHI GCGC 3 cut(s) 825, 1449, 1893
BstMWI GCNNNNNNNGC 1 cut(s) 820
BstNI CCWGG 8 cut(s) 306, 492, 1111, 1310, 1421, 2033, 2217, 2445
BstNSI RCATGY 6 cut(s) 927, 1825, 2104, 2246, 2389, 2505
BstPI GGTNACC 1 cut(s) 295
BstSCI CCNGG 8 cut(s) 304, 490, 1109, 1308, 1419, 2031, 2215, 2443
BstSFI CTRYAG 5 cut(s) 135, 290, 369, 1080, 2394
BstX2I RGATCY 6 cut(s) 365, 562, 1208, 1631, 1923, 1969
BstXI CCANNNNNNTGG 2 cut(s) 35, 990
BstYI RGATCY 6 cut(s) 365, 562, 1208, 1631, 1923, 1969
BsuRI GGCC 6 cut(s) 129, 386, 1308, 2031, 2220, 2380
BtgI CCRYGG 1 cut(s) 659
BtrI CACGTC 1 cut(s) 2149
BtsCI GGATG 6 cut(s) 64, 1000, 1207, 1717, 1784, 2212
BtsI GCAGTG 2 cut(s) 433, 1089
BtsIMutI CAGTG 5 cut(s) 387, 433, 685, 1089, 2005
BveI ACCTGC 3 cut(s) 283, 2241, 2259
Cac8I GCNNGC 8 cut(s) 686, 925, 1220, 1823, 1981, 2286, 2331, 2463
CaiI CAGNNNCTG 1 cut(s) 440
CciI TCATGA 2 cut(s) 739, 1315
CfoI GCGC 3 cut(s) 825, 1449, 1893
Cfr13I GGNCC 6 cut(s) 25, 348, 385, 656, 911, 2271
CsiI ACCWGGT 2 cut(s) 490, 2443
Csp6I GTAC 5 cut(s) 105, 217, 1075, 1380, 2432
CviQI GTAC 5 cut(s) 105, 217, 1075, 1380, 2432
DdeI CTNAG 8 cut(s) 441, 778, 1353, 1597, 1887, 1952, 2020, 2436
EaeI YGGCCR 1 cut(s) 127
Eam1104I CTCTTC 2 cut(s) 1673, 2186
EarI CTCTTC 2 cut(s) 1673, 2186
Eco130I CCWWGG 2 cut(s) 205, 659
Eco147I AGGCCT 1 cut(s) 1308
Eco24I GRGCYC 1 cut(s) 2467
Eco32I GATATC 2 cut(s) 228, 1195
Eco47I GGWCC 5 cut(s) 25, 348, 656, 911, 2271
Eco47III AGCGCT 2 cut(s) 824, 1892
Eco57I CTGAAG 3 cut(s) 1154, 1178, 1733
Eco88I CYCGRG 1 cut(s) 1917
Eco91I GGTNACC 1 cut(s) 295
EcoNI CCTNNNNNAGG 1 cut(s) 570
EcoO65I GGTNACC 1 cut(s) 295
EcoRI GAATTC 2 cut(s) 309, 2003
EcoRII CCWGG 8 cut(s) 304, 490, 1109, 1308, 1419, 2031, 2215, 2443
EcoRV GATATC 2 cut(s) 228, 1195
EcoT14I CCWWGG 2 cut(s) 205, 659
EcoT22I ATGCAT 3 cut(s) 904, 1823, 2248
EcoT38I GRGCYC 1 cut(s) 2467
ErhI CCWWGG 2 cut(s) 205, 659
FalI AAGNNNNNCTT 2 cut(s) 252, 284
FaqI GGGAC 3 cut(s) 2089, 2101, 2315
FauI CCCGC 1 cut(s) 2454
FauNDI CATATG 2 cut(s) 586, 2092
FbaI TGATCA 2 cut(s) 736, 1855
FokI GGATG 6 cut(s) 51, 1007, 1214, 1724, 1791, 2199
FriOI GRGCYC 1 cut(s) 2467
FspBI CTAG 8 cut(s) 8, 572, 1304, 1763, 1775, 1877, 1947, 2037
GlaI GCGC 3 cut(s) 824, 1448, 1892
GsaI CCCAGC 1 cut(s) 391
GsuI CTGGAG 2 cut(s) 789, 2195
HaeII RGCGCY 2 cut(s) 826, 1894
HaeIII GGCC 6 cut(s) 129, 386, 1308, 2031, 2220, 2380
HapII CCGG 1 cut(s) 1435
HhaI GCGC 3 cut(s) 825, 1449, 1893
Hin6I GCGC 3 cut(s) 823, 1447, 1891
HinP1I GCGC 3 cut(s) 823, 1447, 1891
HindIII AAGCTT 3 cut(s) 500, 1834, 1867
HpaII CCGG 1 cut(s) 1435
HphI GGTGA 3 cut(s) 289, 1070, 2426
Hpy166II GTNNAC 1 cut(s) 557
Hpy8I GTNNAC 1 cut(s) 557
HpyCH4III ACNGT 8 cut(s) 554, 896, 938, 1166, 1261, 1327, 1573, 2000
HpyCH4IV ACGT 1 cut(s) 2148
HpyF10VI GCNNNNNNNGC 1 cut(s) 820
HpyF3I CTNAG 8 cut(s) 441, 778, 1353, 1597, 1887, 1952, 2020, 2436
HpySE526I ACGT 1 cut(s) 2148
HspAI GCGC 3 cut(s) 823, 1447, 1891
Ksp22I TGATCA 2 cut(s) 736, 1855
LmnI GCTCC 3 cut(s) 16, 489, 1470
LweI GCATC 5 cut(s) 544, 1304, 1769, 1794, 2160
MabI ACCWGGT 2 cut(s) 490, 2443
MaeI CTAG 8 cut(s) 8, 572, 1304, 1763, 1775, 1877, 1947, 2037
MaeII ACGT 1 cut(s) 2148
MaeIII GTNAC 6 cut(s) 295, 415, 517, 1058, 1729, 2096
MbiI CCGCTC 1 cut(s) 2461
MfeI CAATTG 1 cut(s) 2118
MflI RGATCY 6 cut(s) 365, 562, 1208, 1631, 1923, 1969
MhlI GDGCHC 1 cut(s) 2467
MlsI TGGCCA 1 cut(s) 129
MluNI TGGCCA 1 cut(s) 129
MlyI GAGTC 3 cut(s) 1024, 1337, 2297
MmeI TCCRAC 1 cut(s) 488
Mox20I TGGCCA 1 cut(s) 129
Mph1103I ATGCAT 3 cut(s) 904, 1823, 2248
MscI TGGCCA 1 cut(s) 129
MslI CAYNNNNRTG 3 cut(s) 33, 86, 2500
Msp20I TGGCCA 1 cut(s) 129
MspA1I CMGCKG 2 cut(s) 1056, 2393
MspI CCGG 1 cut(s) 1435
MspR9I CCNGG 8 cut(s) 306, 492, 1111, 1310, 1421, 2033, 2217, 2445
MunI CAATTG 1 cut(s) 2118
Mva1269I GAATGC 4 cut(s) 51, 902, 1785, 1823
MvaI CCWGG 8 cut(s) 306, 492, 1111, 1310, 1421, 2033, 2217, 2445
MwoI GCNNNNNNNGC 1 cut(s) 820
NcoI CCATGG 1 cut(s) 659
NdeI CATATG 2 cut(s) 586, 2092
NlaIV GGNNCC 3 cut(s) 564, 1633, 1925
NmuCI GTSAC 2 cut(s) 295, 1058
NsiI ATGCAT 3 cut(s) 904, 1823, 2248
NspI RCATGY 6 cut(s) 927, 1825, 2104, 2246, 2389, 2505
PaeI GCATGC 2 cut(s) 927, 1825
PagI TCATGA 2 cut(s) 739, 1315
PceI AGGCCT 1 cut(s) 1308
PciI ACATGT 1 cut(s) 2100
PctI GAATGC 4 cut(s) 51, 902, 1785, 1823
PflMI CCANNNNNTGG 3 cut(s) 243, 394, 665
PleI GAGTC 3 cut(s) 1024, 1337, 2297
PpsI GAGTC 3 cut(s) 1024, 1337, 2297
PscI ACATGT 1 cut(s) 2100
PshBI ATTAAT 2 cut(s) 1905, 2310
PsiI TTATAA 1 cut(s) 2454
Psp6I CCWGG 8 cut(s) 304, 490, 1109, 1308, 1419, 2031, 2215, 2443
PspEI GGTNACC 1 cut(s) 295
PspFI CCCAGC 1 cut(s) 387
PspGI CCWGG 8 cut(s) 304, 490, 1109, 1308, 1419, 2031, 2215, 2443
PspN4I GGNNCC 3 cut(s) 564, 1633, 1925
PspPI GGNCC 6 cut(s) 25, 348, 385, 656, 911, 2271
PsrI GAACNNNNNNTAC 2 cut(s) 922, 954
PstI CTGCAG 4 cut(s) 294, 373, 1084, 2398
PstNI CAGNNNCTG 1 cut(s) 440
PsuI RGATCY 6 cut(s) 365, 562, 1208, 1631, 1923, 1969
PvuII CAGCTG 1 cut(s) 2393
RsaI GTAC 5 cut(s) 106, 218, 1076, 1381, 2433
RsaNI GTAC 5 cut(s) 105, 217, 1075, 1380, 2432
RseI CAYNNNNRTG 3 cut(s) 33, 86, 2500
Sau96I GGNCC 6 cut(s) 25, 348, 385, 656, 911, 2271
ScaI AGTACT 1 cut(s) 1076
SchI GAGTC 3 cut(s) 1024, 1337, 2297
ScrFI CCNGG 8 cut(s) 306, 492, 1111, 1310, 1421, 2033, 2217, 2445
SduI GDGCHC 1 cut(s) 2467
SexAI ACCWGGT 2 cut(s) 490, 2443
SfaNI GCATC 5 cut(s) 544, 1304, 1769, 1794, 2160
SfcI CTRYAG 5 cut(s) 135, 290, 369, 1080, 2394
SinI GGWCC 5 cut(s) 25, 348, 656, 911, 2271
SmiMI CAYNNNNRTG 3 cut(s) 33, 86, 2500
SmlI CTYRAG 2 cut(s) 1501, 1701
SmoI CTYRAG 2 cut(s) 1501, 1701
SphI GCATGC 2 cut(s) 927, 1825
SseBI AGGCCT 1 cut(s) 1308
SsiI CCGC 2 cut(s) 1056, 2461
SspI AATATT 1 cut(s) 752
SspMI CTAG 8 cut(s) 8, 572, 1304, 1763, 1775, 1877, 1947, 2037
StuI AGGCCT 1 cut(s) 1308
StyD4I CCNGG 8 cut(s) 304, 490, 1109, 1308, 1419, 2031, 2215, 2443
StyI CCWWGG 2 cut(s) 205, 659
TaaI ACNGT 8 cut(s) 554, 896, 938, 1166, 1261, 1327, 1573, 2000
TaiI ACGT 1 cut(s) 2151
TaqI TCGA 3 cut(s) 1688, 2135, 2535
TatI WGTACW 2 cut(s) 104, 1074
TscAI CASTG 5 cut(s) 387, 433, 685, 1089, 2005
TseFI GTSAC 2 cut(s) 295, 1058
Tsp45I GTSAC 2 cut(s) 295, 1058
TspGWI ACGGA 3 cut(s) 106, 1214, 1624
TspRI CASTG 5 cut(s) 387, 433, 685, 1089, 2005
Van91I CCANNNNNTGG 3 cut(s) 243, 394, 665
VpaK11BI GGWCC 5 cut(s) 25, 348, 656, 911, 2271
VspI ATTAAT 2 cut(s) 1905, 2310
XagI CCTNNNNNAGG 1 cut(s) 570
XapI RAATTY 6 cut(s) 309, 618, 1039, 1186, 1252, 2003
XbaI TCTAGA 1 cut(s) 1946
XceI RCATGY 6 cut(s) 927, 1825, 2104, 2246, 2389, 2505
XspI CTAG 8 cut(s) 8, 572, 1304, 1763, 1775, 1877, 1947, 2037
ZrmI AGTACT 1 cut(s) 1076
Zsp2I ATGCAT 3 cut(s) 904, 1823, 2248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.