Rw5G016930

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000484
Physical Location & Seq
Reverse (-)
21684454 .. 21688535
4082 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G016930.1

Sequence Viewer

Length: 2433 bp
ATGGATGTAACAATAACTCCATTGAAGTGTTTAGTTAGGATCATGTTCTTGCATCTTTTTTCCTGTTTTGTGCTGCTCATGATCACAGCAATGGTTTCCATTGCAGATGACACATACACAAGTACCTTTCAGTCCCTCAGAGATGGCCAGACCCATGTTTCAACTGGTGGAACCTTTGAGCTTGGATTTTTCACTCCTGAAAATATACCCGTTAACACTGTTGTATGGGTTGCCAACAGAGACACACCCCTCACTGACTCATCAGGTGTTCTGAAGATCACCAGTCCTGGAATTCTTGTCCTTGTCGACCATAACAAGACAGTTTGGTCCTCCAACACTTCAAGTCCAGCACAGAATCCAGTGGCTCAGCTTTTGGATTCAGGAAATCTTGTTGTGATAGATAAGAGTGATAATGTTGACCCATCTGCAGAGAGCTTTCTGTGGCAAAGTTTTGACTACCCAGGTGATACATTGCTACCAGGTATGAAACTTGGTAGAAACACAATTACAGGTTTCAAATGGTTTATGTCATCATGGAAAAGTCCCCAGGATCCTTCCCAAGGCAACTATACATTTCAACATGGTCCCAAAGGATATGCAGAGCAGATTATGATGGAGGGTTCGGTCATGAAATTTCGGACTGGACCATGGAATGGCATCCGCTTCAGTGGAGCGCCTCATTTAAGTCCAAATCCAATATACACATACAATCTTCTTTTTGATCATGGTGAAGAATATTATTCTTATAAACTTCTTAACAGGTCAATTCTTTCCAGGCTGGTGTTGGCTCAAGATGGGCTTGTGCACCGATACACATGGATTGATAGAACCCGAGAATGGTTCCTTCATCTAACAGCACAACTTGATAGCTGTGATACCTATGCACTTTGTGGTGCATATGGTGTTGGGTGCTTGAAAGGATTTTCACCTAAATATCCGAAAGATTGGGAAGTGATGGATTGGTCAAATGGTTGTGTCAGAAAGACTCCACTGAGTTGCAATGGAGACATATTTCAGATGTACTATGGGGTGAAATTGCCAAGCACAGAACAATCCTGGTTCAACACAAGTATGAACATCAAGGAATGTGAAATGATGTGCACAAAGAACTGTTCCTGCACAGCTTATTCAAATTTGTATATCAGTCAAGGAGGGACTGGGTGCCGGATGTGGTTTGGTGACCTGGTTGATATTACTATTAGAAATTTCACTAAAAATGGGGAAAATCTCTATATACAAATGGCCGCATCAGAACTACCTACAGACAATACAAAGATCAATGGTCAGAAAGTGAGAATCGTATTAATAATCACTTCTATGTTGTCTACTAGCCTGCTGATATTCTTGACCTTATATCTTCGGAAGAAGCAGCACCAAAAACATGGAAAAGTATGGAGCAGCAAAAAGGAAGATTTGGAACTTCCATTATTTGACTTGGCATCTATTATTTATGCAACCAATAACTTTTCAGAGAACAACAAACTAGGACAGGGAGGTTTTGGATCAGTCTTTAAGACTGATGATTTCAGGGTGTTGAGAGATGGACGTGAAGTAGCTGTGAAAAGGCTCTCAAAGCATTCTAGACAAGGACTAGATGAATTCAAGAATGAGATTATGCATATTGCAAAACTTCAGCACAGGAATCTAGTGAAGCTTCACGGATGCTGCATTCAAGAAGATGAAATGATGCTGATCTATGAATTCATGCCCAACAAAAGCTTGGACTTCTTTATTTTTGATCATACAAAAAGCATGTCACTAGACTGGCGTAAGCGGTTTGACATTATTAATGGCATTGCTCGAGGGCTCCTCTATCTTCATCAAGACTCCAGACTCAGAGTAATTCATCGAGATCTCAAAGCCGGAAATATTTTATTAGACAGTGAATTTATCCCAAAAATATCTGACTTTGGCCTGGCTAGAAGTTTGGGAGGAAATGAAACTAGAGCAGAAACGAATAGAGTGGTTGGCACGTATGGTTATATGTCTCCAGAATATGCAATTGATGGGTTCTACTCTACCAAATCCGACGTCTTTAGCTTTGGTGTTTCGGTGTTGGAGATAATAAGTGGGAAGAGAAACAGAGGATTCTATAATCCAGACCACAGCCTAAACCTCCTTGGACATGCATGGAAGCTATACACACAAGGCAGGTCCATTGAACTGCTTGATACATCAGTAGGGGACTCGACTATTCGACTAGAGGAAGTTCTGCGATCGGTTCATGTAGGACTCTTATGTGTGCAGCAAAATCCAGAAGATAGGCCAAGCATGTCAGCTGCTGTTCTAATGTTGAGTGGTGAAGGTGCATTGCCTCAACCCCAGAAACCAGGTTTTTATACTGAAAGGAATCTGAGTGAGATCAAAGTCGACGCCGCTCCTAAACCATGTTCAGCTAATGAAGTCACTTTTACACTATTGGAGGCCCGATAA

Protein Analysis

810

Amino Acids

91.47

Weight (kDa)

7.03

Isoelectric Point (pI)

39.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 73 - 180 2.6e-37 D-mannose binding lectin
S_locus_glycop PF00954 212 - 309 2e-21 S-locus glycoprotein domain
PAN_2 PF08276 333 - 398 3.8e-18 PAN-like domain
Pkinase PF00069 490 - 695 5.3e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 492 - 761 5e-46 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 768 - 810 7.9e-08 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 747
AasI GACNNNNNNGTC 1 cut(s) 325
AatII GACGTC 1 cut(s) 2034
Acc36I ACCTGC 1 cut(s) 2142
AccB1I GGYRCC 1 cut(s) 1161
AccB7I CCANNNNNTGG 1 cut(s) 653
AccBSI CCGCTC 1 cut(s) 2378
AccI GTMKAC 3 cut(s) 306, 1325, 2370
AciI CCGC 4 cut(s) 661, 1245, 1774, 2376
AclWI GGATC 4 cut(s) 47, 545, 558, 1510
AcoI YGGCCR 2 cut(s) 145, 1242
AcsI RAATTY 7 cut(s) 291, 632, 1132, 1204, 1598, 1700, 1886
AcuI CTGAAG 3 cut(s) 293, 649, 1616
AcyI GRCGYC 2 cut(s) 2031, 2373
AdeI CACNNNGTG 1 cut(s) 890
AfaI GTAC 2 cut(s) 124, 1022
AfiI CCNNNNNNNGG 3 cut(s) 560, 653, 837
AjiI CACGTC 1 cut(s) 1547
AjnI CCWGG 9 cut(s) 286, 460, 478, 546, 773, 1055, 1182, 1914, 2329
Alw21I GWGCWC 2 cut(s) 807, 1103
Alw26I GTCTC 3 cut(s) 234, 999, 1992
Alw44I GTGCAC 2 cut(s) 803, 1099
AlwI GGATC 4 cut(s) 47, 545, 558, 1510
AlwNI CAGNNNCTG 1 cut(s) 2282
Ama87I CYCGRG 2 cut(s) 831, 1800
AoxI GGCC 5 cut(s) 145, 1242, 1912, 2264, 2424
ApaLI GTGCAC 2 cut(s) 803, 1099
ApeKI GCWGC 6 cut(s) 73, 1369, 1398, 1665, 2245, 2279
ApoI RAATTY 7 cut(s) 291, 632, 1132, 1204, 1598, 1700, 1886
ArsI GACNNNNNNTTYG 4 cut(s) 1739, 1771, 2016, 2048
AseI ATTAAT 2 cut(s) 1304, 1788
AspLEI GCGC 1 cut(s) 676
AspS9I GGNCC 5 cut(s) 327, 584, 644, 2154, 2425
AsuHPI GGTGA 7 cut(s) 271, 476, 740, 918, 1042, 1190, 2312
AvaI CYCGRG 2 cut(s) 831, 1800
AvaII GGWCC 4 cut(s) 327, 584, 644, 2154
BaeGI GKGCMC 2 cut(s) 807, 1103
BaeI ACNNNNGTAYC 4 cut(s) 106, 139, 2163, 2196
BalI TGGCCA 1 cut(s) 147
BamHI GGATCC 1 cut(s) 550
BanI GGYRCC 1 cut(s) 1161
BanII GRGCYC 1 cut(s) 1809
Bbv12I GWGCWC 2 cut(s) 807, 1103
BbvI GCAGC 6 cut(s) 60, 1381, 1410, 1652, 2257, 2266
BccI CCATC 7 cut(s) 137, 430, 607, 788, 949, 1535, 2000
BciT130I CCWGG 9 cut(s) 288, 462, 480, 548, 775, 1057, 1184, 1916, 2331
BclI TGATCA 3 cut(s) 81, 721, 1738
BcoDI GTCTC 3 cut(s) 234, 999, 1992
BfaI CTAG 9 cut(s) 1329, 1484, 1581, 1592, 1646, 1760, 1920, 1944, 2201
BfmI CTRYAG 2 cut(s) 426, 1260
BfoI RGCGCY 1 cut(s) 677
BfuAI ACCTGC 1 cut(s) 2142
BglII AGATCT 1 cut(s) 1852
BisI GCNGC 8 cut(s) 74, 1245, 1370, 1399, 1666, 2246, 2280, 2376
BlpI GCTNAGC 1 cut(s) 366
BlsI GCNGC 8 cut(s) 75, 1246, 1371, 1400, 1667, 2247, 2281, 2377
Bme1390I CCNGG 9 cut(s) 288, 462, 480, 548, 775, 1057, 1184, 1916, 2331
Bme18I GGWCC 4 cut(s) 327, 584, 644, 2154
BmeT110I CYCGRG 2 cut(s) 831, 1800
BmgBI CACGTC 1 cut(s) 1547
BmgT120I GGNCC 5 cut(s) 327, 584, 644, 2154, 2425
BmiI GGNNCC 6 cut(s) 172, 552, 586, 842, 1163, 1808
BmrFI CCNGG 9 cut(s) 288, 462, 480, 548, 775, 1057, 1184, 1916, 2331
BmrI ACTGGG 1 cut(s) 1167
BmsI GCATC 6 cut(s) 61, 666, 1256, 1448, 1652, 1677
BmuI ACTGGG 1 cut(s) 1167
BplI GAGNNNNNCTC 2 cut(s) 1794, 1826
BpmI CTGGAG 2 cut(s) 1813, 1974
Bpu1102I GCTNAGC 1 cut(s) 366
BpuEI CTTGAG 1 cut(s) 774
BsaAI YACGTR 1 cut(s) 1974
BsaBI GATNNNNATC 1 cut(s) 1691
BsaHI GRCGYC 2 cut(s) 2031, 2373
BsaJI CCNNGG 5 cut(s) 460, 546, 559, 647, 2119
BsaXI ACNNNNNCTCC 1 cut(s) 31
Bsc4I CCNNNNNNNGG 3 cut(s) 560, 653, 837
Bse1I ACTGG 6 cut(s) 169, 282, 359, 646, 1162, 1769
Bse3DI GCAATG 6 cut(s) 96, 99, 470, 1006, 1794, 2309
Bse8I GATNNNNATC 1 cut(s) 1691
BseBI CCWGG 9 cut(s) 288, 462, 480, 548, 775, 1057, 1184, 1916, 2331
BseDI CCNNGG 5 cut(s) 460, 546, 559, 647, 2119
BseGI GGATG 4 cut(s) 10, 657, 1173, 1667
BseJI GATNNNNATC 1 cut(s) 1691
BseLI CCNNNNNNNGG 3 cut(s) 560, 653, 837
BseMI GCAATG 6 cut(s) 96, 99, 470, 1006, 1794, 2309
BseMII CTCAG 5 cut(s) 151, 380, 983, 1849, 2345
BseNI ACTGG 6 cut(s) 169, 282, 359, 646, 1162, 1769
BseRI GAGGAG 1 cut(s) 1799
BseSI GKGCMC 2 cut(s) 807, 1103
BseXI GCAGC 6 cut(s) 60, 1381, 1410, 1652, 2257, 2266
BsgI GTGCAG 2 cut(s) 1102, 2264
Bsh1285I CGRYCG 1 cut(s) 2219
BshFI GGCC 5 cut(s) 147, 1244, 1914, 2266, 2426
BshNI GGYRCC 1 cut(s) 1161
BsiEI CGRYCG 1 cut(s) 2219
BsiHKAI GWGCWC 2 cut(s) 807, 1103
BsiHKCI CYCGRG 2 cut(s) 831, 1800
BsiSI CCGG 2 cut(s) 1165, 1863
BslFI GGGAC 5 cut(s) 118, 528, 570, 1168, 2198
BslI CCNNNNNNNGG 3 cut(s) 560, 653, 837
BsmAI GTCTC 3 cut(s) 234, 999, 1992
BsmFI GGGAC 5 cut(s) 118, 528, 570, 1168, 2198
BsmI GAATGC 2 cut(s) 1576, 1668
BsnI GGCC 5 cut(s) 147, 1244, 1914, 2266, 2426
BsoBI CYCGRG 2 cut(s) 831, 1800
Bsp1286I GDGCHC 3 cut(s) 807, 1103, 1809
Bsp1720I GCTNAGC 1 cut(s) 366
Bsp19I CCATGG 1 cut(s) 647
BspACI CCGC 4 cut(s) 661, 1245, 1774, 2376
BspANI GGCC 5 cut(s) 147, 1244, 1914, 2266, 2426
BspCNI CTCAG 5 cut(s) 150, 379, 984, 1848, 2346
BspHI TCATGA 2 cut(s) 78, 627
BspLI GGNNCC 6 cut(s) 172, 552, 586, 842, 1163, 1808
BspMAI CTGCAG 1 cut(s) 430
BspMI ACCTGC 1 cut(s) 2142
BspPI GGATC 4 cut(s) 47, 545, 558, 1510
BspT107I GGYRCC 1 cut(s) 1161
BsrBI CCGCTC 1 cut(s) 2378
BsrDI GCAATG 6 cut(s) 96, 99, 470, 1006, 1794, 2309
BsrI ACTGG 6 cut(s) 169, 282, 359, 646, 1162, 1769
BssECI CCNNGG 5 cut(s) 460, 546, 559, 647, 2119
BssNI GRCGYC 2 cut(s) 2031, 2373
BssT1I CCWWGG 3 cut(s) 559, 647, 2119
Bst2UI CCWGG 9 cut(s) 288, 462, 480, 548, 775, 1057, 1184, 1916, 2331
Bst4CI ACNGT 4 cut(s) 220, 322, 1112, 1883
Bst6I CTCTTC 1 cut(s) 2069
BstACI GRCGYC 2 cut(s) 2031, 2373
BstBAI YACGTR 1 cut(s) 1974
BstC8I GCNNGC 1 cut(s) 1334
BstDEI CTNAG 5 cut(s) 137, 366, 992, 1835, 2354
BstDSI CCRYGG 1 cut(s) 647
BstEII GGTNACC 1 cut(s) 1178
BstENI CCTNNNNNAGG 1 cut(s) 558
BstF5I GGATG 4 cut(s) 10, 657, 1173, 1667
BstH2I RGCGCY 1 cut(s) 677
BstHHI GCGC 1 cut(s) 676
BstMAI GTCTC 3 cut(s) 234, 999, 1992
BstMCI CGRYCG 1 cut(s) 2219
BstMWI GCNNNNNNNGC 1 cut(s) 1573
BstNI CCWGG 9 cut(s) 288, 462, 480, 548, 775, 1057, 1184, 1916, 2331
BstNSI RCATGY 3 cut(s) 1756, 2129, 2275
BstPI GGTNACC 1 cut(s) 1178
BstSCI CCNGG 9 cut(s) 286, 460, 478, 546, 773, 1055, 1182, 1914, 2329
BstSFI CTRYAG 2 cut(s) 426, 1260
BstSLI GKGCMC 2 cut(s) 807, 1103
BstV1I GCAGC 6 cut(s) 60, 1381, 1410, 1652, 2257, 2266
BstX2I RGATCY 2 cut(s) 550, 1852
BstXI CCANNNNNNTGG 1 cut(s) 1382
BstYI RGATCY 2 cut(s) 550, 1852
BsuRI GGCC 5 cut(s) 147, 1244, 1914, 2266, 2426
BtgI CCRYGG 1 cut(s) 647
BtrI CACGTC 1 cut(s) 1547
BtsCI GGATG 4 cut(s) 10, 657, 1173, 1667
BtsIMutI CAGTG 6 cut(s) 216, 252, 366, 673, 989, 1888
BveI ACCTGC 1 cut(s) 2142
Cac8I GCNNGC 1 cut(s) 1334
CaiI CAGNNNCTG 1 cut(s) 2282
CciI TCATGA 2 cut(s) 78, 627
CfoI GCGC 1 cut(s) 676
Cfr13I GGNCC 5 cut(s) 327, 584, 644, 2154, 2425
CseI GACGC 1 cut(s) 2381
CsiI ACCWGGT 3 cut(s) 478, 1182, 2329
Csp6I GTAC 2 cut(s) 123, 1021
CviQI GTAC 2 cut(s) 123, 1021
DdeI CTNAG 5 cut(s) 137, 366, 992, 1835, 2354
DraIII CACNNNGTG 1 cut(s) 890
DrdI GACNNNNNNGTC 1 cut(s) 325
DseDI GACNNNNNNGTC 1 cut(s) 325
EaeI YGGCCR 2 cut(s) 145, 1242
Eam1104I CTCTTC 1 cut(s) 2069
EarI CTCTTC 1 cut(s) 2069
Eco130I CCWWGG 3 cut(s) 559, 647, 2119
Eco24I GRGCYC 1 cut(s) 1809
Eco47I GGWCC 4 cut(s) 327, 584, 644, 2154
Eco57I CTGAAG 3 cut(s) 293, 649, 1616
Eco88I CYCGRG 2 cut(s) 831, 1800
Eco91I GGTNACC 1 cut(s) 1178
EcoNI CCTNNNNNAGG 1 cut(s) 558
EcoO65I GGTNACC 1 cut(s) 1178
EcoRI GAATTC 3 cut(s) 291, 1598, 1700
EcoRII CCWGG 9 cut(s) 286, 460, 478, 546, 773, 1055, 1182, 1914, 2329
EcoT14I CCWWGG 3 cut(s) 559, 647, 2119
EcoT22I ATGCAT 2 cut(s) 1620, 2131
EcoT38I GRGCYC 1 cut(s) 1809
ErhI CCWWGG 3 cut(s) 559, 647, 2119
FalI AAGNNNNNCTT 2 cut(s) 783, 815
FaqI GGGAC 5 cut(s) 118, 528, 570, 1168, 2198
FauNDI CATATG 1 cut(s) 898
FbaI TGATCA 3 cut(s) 81, 721, 1738
FblI GTMKAC 3 cut(s) 306, 1325, 2370
Fnu4HI GCNGC 8 cut(s) 74, 1245, 1370, 1399, 1666, 2246, 2280, 2376
FokI GGATG 4 cut(s) 17, 644, 1180, 1674
FriOI GRGCYC 1 cut(s) 1809
Fsp4HI GCNGC 8 cut(s) 74, 1245, 1370, 1399, 1666, 2246, 2280, 2376
FspBI CTAG 9 cut(s) 1329, 1484, 1581, 1592, 1646, 1760, 1920, 1944, 2201
GlaI GCGC 1 cut(s) 675
GluI GCNGC 8 cut(s) 74, 1245, 1370, 1399, 1666, 2246, 2280, 2376
GsuI CTGGAG 2 cut(s) 1813, 1974
HaeII RGCGCY 1 cut(s) 677
HaeIII GGCC 5 cut(s) 147, 1244, 1914, 2266, 2426
HapII CCGG 2 cut(s) 1165, 1863
HgaI GACGC 1 cut(s) 2381
HhaI GCGC 1 cut(s) 676
Hin1I GRCGYC 2 cut(s) 2031, 2373
Hin6I GCGC 1 cut(s) 674
HinP1I GCGC 1 cut(s) 674
HincII GTYRAC 4 cut(s) 214, 307, 418, 2371
HindII GTYRAC 4 cut(s) 214, 307, 418, 2371
HindIII AAGCTT 2 cut(s) 1652, 1717
HpaI GTTAAC 1 cut(s) 214
HpaII CCGG 2 cut(s) 1165, 1863
HphI GGTGA 7 cut(s) 271, 476, 740, 918, 1042, 1190, 2312
Hpy166II GTNNAC 7 cut(s) 214, 307, 418, 805, 1101, 1326, 2371
Hpy8I GTNNAC 7 cut(s) 214, 307, 418, 805, 1101, 1326, 2371
Hpy99I CGWCG 2 cut(s) 2033, 2375
HpyAV CCTTC 3 cut(s) 564, 854, 2297
HpyCH4III ACNGT 4 cut(s) 220, 322, 1112, 1883
HpyCH4IV ACGT 3 cut(s) 1546, 1973, 2031
HpyF10VI GCNNNNNNNGC 1 cut(s) 1573
HpyF3I CTNAG 5 cut(s) 137, 366, 992, 1835, 2354
HpySE526I ACGT 3 cut(s) 1546, 1973, 2031
Hsp92I GRCGYC 2 cut(s) 2031, 2373
HspAI GCGC 1 cut(s) 674
Ksp22I TGATCA 3 cut(s) 81, 721, 1738
KspAI GTTAAC 1 cut(s) 214
LmnI GCTCC 4 cut(s) 671, 1395, 1812, 2383
Lsp1109I GCAGC 6 cut(s) 60, 1381, 1410, 1652, 2257, 2266
LweI GCATC 6 cut(s) 61, 666, 1256, 1448, 1652, 1677
MabI ACCWGGT 3 cut(s) 478, 1182, 2329
MaeI CTAG 9 cut(s) 1329, 1484, 1581, 1592, 1646, 1760, 1920, 1944, 2201
MaeII ACGT 3 cut(s) 1546, 1973, 2031
MaeIII GTNAC 4 cut(s) 7, 1178, 1755, 2404
MbiI CCGCTC 1 cut(s) 2378
MfeI CAATTG 1 cut(s) 2001
MflI RGATCY 2 cut(s) 550, 1852
MhlI GDGCHC 3 cut(s) 807, 1103, 1809
MlsI TGGCCA 1 cut(s) 147
MluNI TGGCCA 1 cut(s) 147
MlyI GAGTC 6 cut(s) 251, 979, 1820, 1827, 2180, 2226
MmeI TCCRAC 3 cut(s) 357, 2037, 2052
Mox20I TGGCCA 1 cut(s) 147
Mph1103I ATGCAT 2 cut(s) 1620, 2131
MscI TGGCCA 1 cut(s) 147
MseI TTAA 6 cut(s) 213, 683, 756, 1304, 1512, 1788
MslI CAYNNNNRTG 4 cut(s) 25, 89, 1070, 1316
Msp20I TGGCCA 1 cut(s) 147
MspA1I CMGCKG 1 cut(s) 2279
MspI CCGG 2 cut(s) 1165, 1863
MspR9I CCNGG 9 cut(s) 288, 462, 480, 548, 775, 1057, 1184, 1916, 2331
MunI CAATTG 1 cut(s) 2001
Mva1269I GAATGC 2 cut(s) 1576, 1668
MvaI CCWGG 9 cut(s) 288, 462, 480, 548, 775, 1057, 1184, 1916, 2331
MwoI GCNNNNNNNGC 1 cut(s) 1573
NcoI CCATGG 1 cut(s) 647
NdeI CATATG 1 cut(s) 898
NlaIV GGNNCC 6 cut(s) 172, 552, 586, 842, 1163, 1808
NmuCI GTSAC 3 cut(s) 1178, 1755, 2404
NsiI ATGCAT 2 cut(s) 1620, 2131
NspI RCATGY 3 cut(s) 1756, 2129, 2275
PaeR7I CTCGAG 1 cut(s) 1800
PagI TCATGA 2 cut(s) 78, 627
PctI GAATGC 2 cut(s) 1576, 1668
PfeI GAWTC 6 cut(s) 355, 377, 1296, 1642, 2088, 2350
PflMI CCANNNNNTGG 1 cut(s) 653
PfoI TCCNGGA 1 cut(s) 286
PkrI GCNGC 8 cut(s) 75, 1246, 1371, 1400, 1667, 2247, 2281, 2377
Ple19I CGATCG 1 cut(s) 2219
PleI GAGTC 6 cut(s) 251, 979, 1820, 1827, 2180, 2226
PpsI GAGTC 6 cut(s) 251, 979, 1820, 1827, 2180, 2226
Ppu21I YACGTR 1 cut(s) 1974
PshBI ATTAAT 2 cut(s) 1304, 1788
PsiI TTATAA 1 cut(s) 747
Psp6I CCWGG 9 cut(s) 286, 460, 478, 546, 773, 1055, 1182, 1914, 2329
PspEI GGTNACC 1 cut(s) 1178
PspGI CCWGG 9 cut(s) 286, 460, 478, 546, 773, 1055, 1182, 1914, 2329
PspN4I GGNNCC 6 cut(s) 172, 552, 586, 842, 1163, 1808
PspPI GGNCC 5 cut(s) 327, 584, 644, 2154, 2425
PspXI VCTCGAGB 1 cut(s) 1800
PstI CTGCAG 1 cut(s) 430
PstNI CAGNNNCTG 1 cut(s) 2282
PsuI RGATCY 2 cut(s) 550, 1852
PvuI CGATCG 1 cut(s) 2219
PvuII CAGCTG 1 cut(s) 2279
RsaI GTAC 2 cut(s) 124, 1022
RsaNI GTAC 2 cut(s) 123, 1021
RseI CAYNNNNRTG 4 cut(s) 25, 89, 1070, 1316
SalI GTCGAC 2 cut(s) 305, 2369
SaqAI TTAA 6 cut(s) 213, 683, 756, 1304, 1512, 1788
SatI GCNGC 8 cut(s) 74, 1245, 1370, 1399, 1666, 2246, 2280, 2376
Sau96I GGNCC 5 cut(s) 327, 584, 644, 2154, 2425
SchI GAGTC 6 cut(s) 251, 979, 1820, 1827, 2180, 2226
ScrFI CCNGG 9 cut(s) 288, 462, 480, 548, 775, 1057, 1184, 1916, 2331
SduI GDGCHC 3 cut(s) 807, 1103, 1809
SexAI ACCWGGT 3 cut(s) 478, 1182, 2329
SfaNI GCATC 6 cut(s) 61, 666, 1256, 1448, 1652, 1677
SfcI CTRYAG 2 cut(s) 426, 1260
Sfr274I CTCGAG 1 cut(s) 1800
SinI GGWCC 4 cut(s) 327, 584, 644, 2154
SlaI CTCGAG 1 cut(s) 1800
SmiMI CAYNNNNRTG 4 cut(s) 25, 89, 1070, 1316
SmlI CTYRAG 2 cut(s) 789, 1800
SmoI CTYRAG 2 cut(s) 789, 1800
SsiI CCGC 4 cut(s) 661, 1245, 1774, 2376
SspI AATATT 2 cut(s) 737, 1870
SspMI CTAG 9 cut(s) 1329, 1484, 1581, 1592, 1646, 1760, 1920, 1944, 2201
StyD4I CCNGG 9 cut(s) 286, 460, 478, 546, 773, 1055, 1182, 1914, 2329
StyI CCWWGG 3 cut(s) 559, 647, 2119
TaaI ACNGT 4 cut(s) 220, 322, 1112, 1883
TaiI ACGT 3 cut(s) 1549, 1976, 2034
TaqI TCGA 6 cut(s) 306, 1801, 1849, 2189, 2197, 2370
TaqII GACCGA 1 cut(s) 613
TatI WGTACW 1 cut(s) 1020
TauI GCSGC 2 cut(s) 1247, 2378
TfiI GAWTC 6 cut(s) 355, 377, 1296, 1642, 2088, 2350
Tru1I TTAA 6 cut(s) 213, 683, 756, 1304, 1512, 1788
Tru9I TTAA 6 cut(s) 213, 683, 756, 1304, 1512, 1788
TscAI CASTG 6 cut(s) 223, 259, 366, 673, 996, 1888
TseFI GTSAC 3 cut(s) 1178, 1755, 2404
TseI GCWGC 6 cut(s) 73, 1369, 1398, 1665, 2245, 2279
Tsp45I GTSAC 3 cut(s) 1178, 1755, 2404
TspGWI ACGGA 1 cut(s) 1674
TspRI CASTG 6 cut(s) 223, 259, 366, 673, 996, 1888
Van91I CCANNNNNTGG 1 cut(s) 653
VneI GTGCAC 2 cut(s) 803, 1099
VpaK11BI GGWCC 4 cut(s) 327, 584, 644, 2154
VspI ATTAAT 2 cut(s) 1304, 1788
XagI CCTNNNNNAGG 1 cut(s) 558
XapI RAATTY 7 cut(s) 291, 632, 1132, 1204, 1598, 1700, 1886
XbaI TCTAGA 1 cut(s) 1580
XceI RCATGY 3 cut(s) 1756, 2129, 2275
XcmI CCANNNNNNNNNTGG 3 cut(s) 161, 781, 1717
XhoI CTCGAG 1 cut(s) 1800
XmiI GTMKAC 3 cut(s) 306, 1325, 2370
XspI CTAG 9 cut(s) 1329, 1484, 1581, 1592, 1646, 1760, 1920, 1944, 2201
ZraI GACGTC 1 cut(s) 2032
Zsp2I ATGCAT 2 cut(s) 1620, 2131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.