pycom05g20170

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000484
Physical Location & Seq
Reverse (-)
23031098 .. 23034353
3256 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g20170.5

Sequence Viewer

Length: 2448 bp
ATGTGCTTGATCCTCTTCTACTCTTCTCTGCTGCTCATCTTCGCAGCAGTATTCTCCATAGCTGATGACGCCACGAGTACATTTCAGTCCATTAGAGACGGTGAGACTGTAGTTTCAACTGGCGGTACGTTTGAGCTAGGATTTTGCAGTCCTGATGCTTCTAATAGACGGTACGTGGGGATATGGTACAAGCACATATCTGTTACAACCATTGTATGGGTTGCCAACAGAGACAAGCCCCTCGCTGATTTGTCCGGCGTTCTGAAGGTCACCAACCCTGGAATTCTTGTCCTCAACCACAACATGAGCACAGTTTGGTCCTCCAACACATCGAGAACTGCACAGAATCCAGTGGCACGTCTTTTGGATTCGGGTAATCTTGTCGTGATAGATAGGAGTGATGATGACTCTGAGAACTTTCTGTGGCAAAGTTTTGATTACCCTGGCGACACATTCTTACCAGGTATGAAGATTGGTAGGAACACGGTTACAGGCTTCAATTGGCATCTTAGATCGTGGAAAACTCCTCAGGATCCTTCTCAAGGCAATTGTACAAATCAACTTGGTCCCAAAGGATATGCAGAATTATTTGTGAGGGAAGGTTCGGTCATTAAATTTCGGTCTGGACCATGGAATGGAGTCTGGTTCAGTGGAAACCCTCAGTTAAATCAAAACTCTATATACACATACAGTCTTGTTTCTGAGCGTGATGAAATGTACTACAGTTACAAGCTTCACAACAGCTCAATCTTTTCAAGGTTGATGTTAACTTCAGATGGACTTCTGCAGCGCTACACATGGATTGATAGAACCAAAGGTTGGGTTCTTTACCAAACAGTCCAAATTGATGACTGTGACAACTATGCATTGTGTGGTGTACATGGTGCATGTAACATTCAAAAGTCCCCGGTTTGTAGCTGTTTGAAAGGATTTACACCAAAGTTTCCGAAAGAATGGGATTTGGCAGATTGGTCTAATGGTTGTGTGAGAAAGACTTCTCTAAATTGCACCGGAGACGTGTTCCAAAAGTACTCGGGGTTGAAATTGCCTAGCACAGAACAATCCTGGTTTAACAAAAGTATGAACCTCAAGGAATGTGAGATGGTGTGCATGAAGAACTGCTCCTGCACGGCTTATTCAAATTTGGATATCCGGGATGGAGGAACTGGGTGCTTGCTGTGGTACGGTGATCTAATTGATATAGTATACATTGCTGAAAACGTGCAAGATATTTATATAAGAATGGCCGCAGCAGAAAAAGATCATGAAGACGATACAAAGATCAACGCTAAATACTCTGAAACCAATGCAAAGAAAATGAGAATCATAATAAGCACTACTATGTTGTCTATCGGACTGCTGATCATGGGCCTTACCCTGTTGTTTTATGTTTGGAAGAAGCAGCACCAAAAAGGAAAACTGGGACGCAACCAAAATGAGGATCTGGAATTACCGTTATTTGATTTGATGACTGTAGTTTCTGCAACCGGTAACTTTTCAATTGAAAACAAACTTGGTGAAGGCGGTTTCGGACCTGTCTTTAAGGGTACGATGGAAGATGGACAAGAAATCGCAGTGAAAAGGCTTTCAAAAAGTTCTAGACAAGGACTCGACGAGTTCAAGAATGAAGTTACACATATTGCCAAACTTCAGCACAGGAATCTAGTGAAGCTTCTTGGATGCTGCATTCAAGAAGACGAGATGATACTGATCTACGAGTTCATGCCCAACAAGAGCTTAGACTTATTTATTTTCGATCAAAGAAGAAGGATGTTATTAGACTGGCCGAAGCGCTTTGAAATTATTAATGGGATAGCTCGAGGGCTCCTCTATCTTCATCAAGATTCTAGATTGAGAGTTATTCATAGAGATCTCAAAGCAAGTAACATTTTATTAGGCAATGAATTTAACCCGAAAATCTCAGACTTTGGCCTGGCTAGAAGCTTTGGAGGAAATGAAACGAAAGCAAAAACAAAGAAAGTGGTCGGAACATATGGTTACATGTCCCCAGAATATACAATTGATGGTTTCTACTCTATAAAGTCCGATGTCTATAGCTTTGGTGTTCTAGTGCTAGAGATAGTGAGCGGGAGCAGAAATAGAGGATTCTCTCATCCAGACCACAAACTCAACCTTCTTGGACATGCATGGATGCTACACACAGAAGGCAGGCCTCTCAAACTGCTTGATGCATCAGTAGAGGACTCTGTCACTCTGCATGAAGTTGTAAGAACGATTCATGTGGGTCTTTTGTGCGTGCAGCGGAATCCAGAAGACAGACCGAGCATGTCAGCTATAGTTCTAATGTTGGGTGGTGAAGGTGTGTTGCCCCCACCTCTAAAACCTGGTTTTTATAGTGAAAGGGATCTAACTGAACTCGAAACCAATTATTCTTCTAAAGCATGCTCAGCTAATGAAGTCACTATTTCACTAGTTGAGGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

816

Amino Acids

91.83

Weight (kDa)

6.79

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 216, 635, 819
AccBSI CCGCTC 1 cut(s) 2088
AccI GTMKAC 1 cut(s) 1206
AciI CCGC 5 cut(s) 123, 1248, 1524, 2088, 2263
AclWI GGATC 5 cut(s) 4, 527, 540, 1449, 2373
AcoI YGGCCR 2 cut(s) 1245, 1784
AcsI RAATTY 4 cut(s) 282, 614, 1141, 1904
AcuI CTGAAG 3 cut(s) 284, 756, 1634
AcyI GRCGYC 1 cut(s) 69
AfeI AGCGCT 2 cut(s) 791, 1793
AfiI CCNNNNNNNGG 5 cut(s) 216, 542, 635, 819, 1438
AflIII ACRYGT 2 cut(s) 1017, 2001
AgeI ACCGGT 1 cut(s) 1487
AhlI ACTAGT 1 cut(s) 2431
AjiI CACGTC 2 cut(s) 359, 1018
AjnI CCWGG 6 cut(s) 277, 442, 460, 1064, 1932, 2344
AjuI GAANNNNNNNTTGG 4 cut(s) 802, 834, 1497, 1529
Alw21I GWGCWC 1 cut(s) 311
Alw26I GTCTC 4 cut(s) 90, 98, 225, 1008
AlwI GGATC 5 cut(s) 4, 527, 540, 1449, 2373
Ama87I CYCGRG 2 cut(s) 1033, 1818
Aor51HI AGCGCT 2 cut(s) 791, 1793
AoxI GGCC 5 cut(s) 1245, 1369, 1784, 1930, 2171
ApeKI GCWGC 7 cut(s) 31, 44, 787, 1250, 1402, 1683, 2260
ApoI RAATTY 4 cut(s) 282, 614, 1141, 1904
AseI ATTAAT 1 cut(s) 1806
AsiGI ACCGGT 1 cut(s) 1487
Asp700I GAANNNNTTC 1 cut(s) 994
AspLEI GCGC 2 cut(s) 792, 1794
AspS9I GGNCC 5 cut(s) 318, 566, 626, 1369, 1532
AsuC2I CCSGG 2 cut(s) 908, 1154
AsuHPI GGTGA 5 cut(s) 113, 262, 1199, 1529, 2327
AvaI CYCGRG 2 cut(s) 1033, 1818
AvaII GGWCC 4 cut(s) 318, 566, 626, 1532
AxyI CCTNAGG 1 cut(s) 528
BamHI GGATCC 1 cut(s) 532
BanII GRGCYC 1 cut(s) 1827
BauI CACGAG 1 cut(s) 73
BbsI GAAGAC 3 cut(s) 1275, 1701, 2280
Bbv12I GWGCWC 1 cut(s) 311
BbvI GCAGC 7 cut(s) 18, 56, 799, 1262, 1414, 1670, 2272
BccI CCATC 6 cut(s) 770, 1096, 1151, 1546, 1553, 2018
BceAI ACGGC 1 cut(s) 1146
BciT130I CCWGG 6 cut(s) 279, 444, 462, 1066, 1934, 2346
BclI TGATCA 1 cut(s) 1362
BcnI CCSGG 2 cut(s) 908, 1154
BcoDI GTCTC 4 cut(s) 90, 98, 225, 1008
BcuI ACTAGT 1 cut(s) 2431
BfaI CTAG 9 cut(s) 137, 1050, 1599, 1664, 1848, 1938, 2069, 2075, 2432
BfmI CTRYAG 6 cut(s) 108, 721, 785, 1473, 2053, 2295
BfoI RGCGCY 2 cut(s) 793, 1795
BglII AGATCT 1 cut(s) 1870
BisI GCNGC 8 cut(s) 32, 45, 788, 1248, 1251, 1403, 1684, 2261
BlpI GCTNAGC 1 cut(s) 2407
BlsI GCNGC 8 cut(s) 33, 46, 789, 1249, 1252, 1404, 1685, 2262
BmcAI AGTACT 1 cut(s) 1031
Bme1390I CCNGG 8 cut(s) 279, 444, 462, 908, 1066, 1154, 1934, 2346
Bme18I GGWCC 4 cut(s) 318, 566, 626, 1532
BmeT110I CYCGRG 2 cut(s) 1033, 1818
BmgBI CACGTC 2 cut(s) 359, 1018
BmgT120I GGNCC 5 cut(s) 318, 566, 626, 1369, 1532
BmiI GGNNCC 3 cut(s) 534, 568, 1826
BmrFI CCNGG 8 cut(s) 279, 444, 462, 908, 1066, 1154, 1934, 2346
BmrI ACTGGG 2 cut(s) 1176, 1430
BmsI GCATC 6 cut(s) 145, 514, 1670, 2142, 2179, 2201
BmuI ACTGGG 2 cut(s) 1176, 1430
BpiI GAAGAC 3 cut(s) 1275, 1701, 2280
BplI GAGNNNNNCTC 2 cut(s) 1812, 1844
Bpu1102I GCTNAGC 1 cut(s) 2407
BpuEI CTTGAG 2 cut(s) 525, 1073
BpuMI CCSGG 2 cut(s) 908, 1154
BsaAI YACGTR 1 cut(s) 175
BsaBI GATNNNNATC 1 cut(s) 1709
BsaHI GRCGYC 1 cut(s) 69
BsaJI CCNNGG 4 cut(s) 277, 442, 629, 906
BsaWI WCCGGW 2 cut(s) 1010, 1487
Bsc4I CCNNNNNNNGG 5 cut(s) 216, 542, 635, 819, 1438
Bse118I RCCGGY 1 cut(s) 1487
Bse1I ACTGG 5 cut(s) 124, 350, 1171, 1425, 1787
Bse21I CCTNAGG 1 cut(s) 528
Bse3DI GCAATG 2 cut(s) 1209, 1906
Bse8I GATNNNNATC 1 cut(s) 1709
BseBI CCWGG 6 cut(s) 279, 444, 462, 1066, 1934, 2346
BseDI CCNNGG 4 cut(s) 277, 442, 629, 906
BseGI GGATG 5 cut(s) 1162, 1685, 1776, 2113, 2157
BseJI GATNNNNATC 1 cut(s) 1709
BseLI CCNNNNNNNGG 5 cut(s) 216, 542, 635, 819, 1438
BseMI GCAATG 2 cut(s) 1209, 1906
BseMII CTCAG 6 cut(s) 402, 542, 674, 693, 1935, 2421
BseNI ACTGG 5 cut(s) 124, 350, 1171, 1425, 1787
BseRI GAGGAG 2 cut(s) 516, 1817
BseXI GCAGC 7 cut(s) 18, 56, 799, 1262, 1414, 1670, 2272
BsgI GTGCAG 3 cut(s) 324, 1111, 2279
BshFI GGCC 5 cut(s) 1247, 1371, 1786, 1932, 2173
BshTI ACCGGT 1 cut(s) 1487
BsiHKAI GWGCWC 1 cut(s) 311
BsiHKCI CYCGRG 2 cut(s) 1033, 1818
BsiSI CCGG 5 cut(s) 255, 908, 1011, 1153, 1488
BslFI GGGAC 4 cut(s) 552, 889, 1437, 1990
BslI CCNNNNNNNGG 5 cut(s) 216, 542, 635, 819, 1438
BsmAI GTCTC 4 cut(s) 90, 98, 225, 1008
BsmBI CGTCTC 2 cut(s) 90, 1008
BsmFI GGGAC 4 cut(s) 552, 889, 1437, 1990
BsmI GAATGC 1 cut(s) 1686
BsnI GGCC 5 cut(s) 1247, 1371, 1786, 1932, 2173
BsoBI CYCGRG 2 cut(s) 1033, 1818
Bsp1286I GDGCHC 2 cut(s) 311, 1827
Bsp1407I TGTACA 2 cut(s) 551, 877
Bsp1720I GCTNAGC 1 cut(s) 2407
Bsp19I CCATGG 1 cut(s) 629
BspACI CCGC 5 cut(s) 123, 1248, 1524, 2088, 2263
BspANI GGCC 5 cut(s) 1247, 1371, 1786, 1932, 2173
BspCNI CTCAG 6 cut(s) 403, 541, 673, 694, 1934, 2420
BspHI TCATGA 1 cut(s) 1264
BspLI GGNNCC 3 cut(s) 534, 568, 1826
BspMAI CTGCAG 1 cut(s) 789
BspPI GGATC 5 cut(s) 4, 527, 540, 1449, 2373
BsrBI CCGCTC 1 cut(s) 2088
BsrDI GCAATG 2 cut(s) 1209, 1906
BsrFI RCCGGY 1 cut(s) 1487
BsrGI TGTACA 2 cut(s) 551, 877
BsrI ACTGG 5 cut(s) 124, 350, 1171, 1425, 1787
BssAI RCCGGY 1 cut(s) 1487
BssECI CCNNGG 4 cut(s) 277, 442, 629, 906
BssNAI GTATAC 1 cut(s) 1207
BssNI GRCGYC 1 cut(s) 69
BssSI CACGAG 1 cut(s) 73
BssT1I CCWWGG 1 cut(s) 629
Bst1107I GTATAC 1 cut(s) 1207
Bst2BI CACGAG 1 cut(s) 73
Bst2UI CCWGG 6 cut(s) 279, 444, 462, 1066, 1934, 2346
Bst6I CTCTTC 2 cut(s) 20, 28
BstACI GRCGYC 1 cut(s) 69
BstAUI TGTACA 2 cut(s) 551, 877
BstBAI YACGTR 1 cut(s) 175
BstC8I GCNNGC 4 cut(s) 1175, 2171, 2258, 2404
BstDEI CTNAG 8 cut(s) 411, 509, 528, 660, 702, 1738, 1921, 2407
BstDSI CCRYGG 1 cut(s) 629
BstEII GGTNACC 1 cut(s) 268
BstENI CCTNNNNNAGG 1 cut(s) 540
BstF5I GGATG 5 cut(s) 1162, 1685, 1776, 2113, 2157
BstH2I RGCGCY 2 cut(s) 793, 1795
BstHHI GCGC 2 cut(s) 792, 1794
BstMAI GTCTC 4 cut(s) 90, 98, 225, 1008
BstMWI GCNNNNNNNGC 2 cut(s) 68, 2408
BstNI CCWGG 6 cut(s) 279, 444, 462, 1066, 1934, 2346
BstNSI RCATGY 5 cut(s) 891, 2005, 2147, 2290, 2406
BstPI GGTNACC 1 cut(s) 268
BstSCI CCNGG 8 cut(s) 277, 442, 460, 906, 1064, 1152, 1932, 2344
BstSFI CTRYAG 6 cut(s) 108, 721, 785, 1473, 2053, 2295
BstV1I GCAGC 7 cut(s) 18, 56, 799, 1262, 1414, 1670, 2272
BstV2I GAAGAC 3 cut(s) 1275, 1701, 2280
BstX2I RGATCY 4 cut(s) 532, 1441, 1870, 2365
BstYI RGATCY 4 cut(s) 532, 1441, 1870, 2365
BstZ17I GTATAC 1 cut(s) 1207
Bsu36I CCTNAGG 1 cut(s) 528
BsuRI GGCC 5 cut(s) 1247, 1371, 1786, 1932, 2173
BtgI CCRYGG 1 cut(s) 629
BtrI CACGTC 2 cut(s) 359, 1018
BtsCI GGATG 5 cut(s) 1162, 1685, 1776, 2113, 2157
BtsI GCAGTG 1 cut(s) 1581
BtsIMutI CAGTG 3 cut(s) 357, 655, 1581
Cac8I GCNNGC 4 cut(s) 1175, 2171, 2258, 2404
CciI TCATGA 1 cut(s) 1264
CfoI GCGC 2 cut(s) 792, 1794
Cfr10I RCCGGY 1 cut(s) 1487
Cfr13I GGNCC 5 cut(s) 318, 566, 626, 1369, 1532
CseI GACGC 2 cut(s) 77, 1434
CsiI ACCWGGT 2 cut(s) 460, 2344
CspAI ACCGGT 1 cut(s) 1487
CspCI CAANNNNNGTGG 2 cut(s) 1962, 1997
DdeI CTNAG 8 cut(s) 411, 509, 528, 660, 702, 1738, 1921, 2407
EaeI YGGCCR 2 cut(s) 1245, 1784
Eam1104I CTCTTC 2 cut(s) 20, 28
EarI CTCTTC 2 cut(s) 20, 28
Eco130I CCWWGG 1 cut(s) 629
Eco147I AGGCCT 1 cut(s) 2173
Eco24I GRGCYC 1 cut(s) 1827
Eco32I GATATC 1 cut(s) 1150
Eco47I GGWCC 4 cut(s) 318, 566, 626, 1532
Eco47III AGCGCT 2 cut(s) 791, 1793
Eco57I CTGAAG 3 cut(s) 284, 756, 1634
Eco81I CCTNAGG 1 cut(s) 528
Eco88I CYCGRG 2 cut(s) 1033, 1818
Eco91I GGTNACC 1 cut(s) 268
EcoNI CCTNNNNNAGG 1 cut(s) 540
EcoO65I GGTNACC 1 cut(s) 268
EcoRI GAATTC 1 cut(s) 282
EcoRII CCWGG 6 cut(s) 277, 442, 460, 1064, 1932, 2344
EcoRV GATATC 1 cut(s) 1150
EcoT14I CCWWGG 1 cut(s) 629
EcoT22I ATGCAT 3 cut(s) 868, 2149, 2194
EcoT38I GRGCYC 1 cut(s) 1827
ErhI CCWWGG 1 cut(s) 629
Esp3I CGTCTC 2 cut(s) 90, 1008
FaqI GGGAC 4 cut(s) 552, 889, 1437, 1990
FauI CCCGC 1 cut(s) 2081
FauNDI CATATG 1 cut(s) 1993
FbaI TGATCA 1 cut(s) 1362
FblI GTMKAC 1 cut(s) 1206
Fnu4HI GCNGC 8 cut(s) 32, 45, 788, 1248, 1251, 1403, 1684, 2261
FokI GGATG 5 cut(s) 1169, 1692, 1783, 2100, 2164
FriOI GRGCYC 1 cut(s) 1827
Fsp4HI GCNGC 8 cut(s) 32, 45, 788, 1248, 1251, 1403, 1684, 2261
FspBI CTAG 9 cut(s) 137, 1050, 1599, 1664, 1848, 1938, 2069, 2075, 2432
GlaI GCGC 2 cut(s) 791, 1793
GluI GCNGC 8 cut(s) 32, 45, 788, 1248, 1251, 1403, 1684, 2261
HaeII RGCGCY 2 cut(s) 793, 1795
HaeIII GGCC 5 cut(s) 1247, 1371, 1786, 1932, 2173
HapII CCGG 5 cut(s) 255, 908, 1011, 1153, 1488
HgaI GACGC 2 cut(s) 77, 1434
HhaI GCGC 2 cut(s) 792, 1794
Hin1I GRCGYC 1 cut(s) 69
Hin6I GCGC 2 cut(s) 790, 1792
HinP1I GCGC 2 cut(s) 790, 1792
HincII GTYRAC 1 cut(s) 768
HindII GTYRAC 1 cut(s) 768
HindIII AAGCTT 3 cut(s) 731, 1670, 1942
HpaI GTTAAC 1 cut(s) 768
HpaII CCGG 5 cut(s) 255, 908, 1011, 1153, 1488
HphI GGTGA 5 cut(s) 113, 262, 1199, 1529, 2327
Hpy166II GTNNAC 3 cut(s) 768, 878, 1207
Hpy8I GTNNAC 3 cut(s) 768, 878, 1207
Hpy99I CGWCG 1 cut(s) 1616
HpyAV CCTTC 8 cut(s) 259, 546, 593, 1514, 1761, 2144, 2159, 2312
HpyCH4IV ACGT 5 cut(s) 128, 174, 358, 1017, 1221
HpyF10VI GCNNNNNNNGC 2 cut(s) 68, 2408
HpyF3I CTNAG 8 cut(s) 411, 509, 528, 660, 702, 1738, 1921, 2407
HpySE526I ACGT 5 cut(s) 128, 174, 358, 1017, 1221
Hsp92I GRCGYC 1 cut(s) 69
HspAI GCGC 2 cut(s) 790, 1792
Ksp22I TGATCA 1 cut(s) 1362
KspAI GTTAAC 1 cut(s) 768
LmnI GCTCC 3 cut(s) 1127, 1830, 2091
Lsp1109I GCAGC 7 cut(s) 18, 56, 799, 1262, 1414, 1670, 2272
LweI GCATC 6 cut(s) 145, 514, 1670, 2142, 2179, 2201
MabI ACCWGGT 2 cut(s) 460, 2344
MaeI CTAG 9 cut(s) 137, 1050, 1599, 1664, 1848, 1938, 2069, 2075, 2432
MaeII ACGT 5 cut(s) 128, 174, 358, 1017, 1221
MbiI CCGCTC 1 cut(s) 2088
MfeI CAATTG 4 cut(s) 499, 547, 1500, 2019
MflI RGATCY 4 cut(s) 532, 1441, 1870, 2365
MhlI GDGCHC 2 cut(s) 311, 1827
MlyI GAGTC 4 cut(s) 401, 648, 1602, 2198
MmeI TCCRAC 2 cut(s) 348, 1966
Mph1103I ATGCAT 3 cut(s) 868, 2149, 2194
MroXI GAANNNNTTC 1 cut(s) 994
MseI TTAA 7 cut(s) 612, 665, 767, 1071, 1542, 1806, 1908
MslI CAYNNNNRTG 1 cut(s) 1340
MspA1I CMGCKG 1 cut(s) 2263
MspI CCGG 5 cut(s) 255, 908, 1011, 1153, 1488
MspR9I CCNGG 8 cut(s) 279, 444, 462, 908, 1066, 1154, 1934, 2346
MunI CAATTG 4 cut(s) 499, 547, 1500, 2019
Mva1269I GAATGC 1 cut(s) 1686
MvaI CCWGG 6 cut(s) 279, 444, 462, 1066, 1934, 2346
MwoI GCNNNNNNNGC 2 cut(s) 68, 2408
NciI CCSGG 2 cut(s) 908, 1154
NcoI CCATGG 1 cut(s) 629
NdeI CATATG 1 cut(s) 1993
NlaIV GGNNCC 3 cut(s) 534, 568, 1826
NmuCI GTSAC 4 cut(s) 268, 854, 2209, 2419
NsiI ATGCAT 3 cut(s) 868, 2149, 2194
NspI RCATGY 5 cut(s) 891, 2005, 2147, 2290, 2406
PaeI GCATGC 1 cut(s) 2406
PaeR7I CTCGAG 1 cut(s) 1818
PagI TCATGA 1 cut(s) 1264
PceI AGGCCT 1 cut(s) 2173
PciI ACATGT 1 cut(s) 2001
PctI GAATGC 1 cut(s) 1686
PdmI GAANNNNTTC 1 cut(s) 994
PfeI GAWTC 8 cut(s) 346, 368, 1323, 1660, 1844, 2106, 2236, 2266
PflFI GACNNNGTC 1 cut(s) 2207
PflMI CCANNNNNTGG 3 cut(s) 216, 635, 819
PfoI TCCNGGA 1 cut(s) 1152
PinAI ACCGGT 1 cut(s) 1487
PkrI GCNGC 8 cut(s) 33, 46, 789, 1249, 1252, 1404, 1685, 2262
PleI GAGTC 4 cut(s) 401, 647, 1602, 2198
PpsI GAGTC 4 cut(s) 401, 647, 1602, 2198
Ppu21I YACGTR 1 cut(s) 175
PscI ACATGT 1 cut(s) 2001
PshBI ATTAAT 1 cut(s) 1806
Psp6I CCWGG 6 cut(s) 277, 442, 460, 1064, 1932, 2344
PspEI GGTNACC 1 cut(s) 268
PspGI CCWGG 6 cut(s) 277, 442, 460, 1064, 1932, 2344
PspN4I GGNNCC 3 cut(s) 534, 568, 1826
PspPI GGNCC 5 cut(s) 318, 566, 626, 1369, 1532
PspXI VCTCGAGB 1 cut(s) 1818
PstI CTGCAG 1 cut(s) 789
PsuI RGATCY 4 cut(s) 532, 1441, 1870, 2365
PsyI GACNNNGTC 1 cut(s) 2207
RseI CAYNNNNRTG 1 cut(s) 1340
SaqAI TTAA 7 cut(s) 612, 665, 767, 1071, 1542, 1806, 1908
SatI GCNGC 8 cut(s) 32, 45, 788, 1248, 1251, 1403, 1684, 2261
Sau96I GGNCC 5 cut(s) 318, 566, 626, 1369, 1532
ScaI AGTACT 1 cut(s) 1031
SchI GAGTC 4 cut(s) 401, 648, 1602, 2198
ScrFI CCNGG 8 cut(s) 279, 444, 462, 908, 1066, 1154, 1934, 2346
SduI GDGCHC 2 cut(s) 311, 1827
SexAI ACCWGGT 2 cut(s) 460, 2344
SfaNI GCATC 6 cut(s) 145, 514, 1670, 2142, 2179, 2201
SfcI CTRYAG 6 cut(s) 108, 721, 785, 1473, 2053, 2295
Sfr274I CTCGAG 1 cut(s) 1818
SinI GGWCC 4 cut(s) 318, 566, 626, 1532
SlaI CTCGAG 1 cut(s) 1818
SmiMI CAYNNNNRTG 1 cut(s) 1340
SmlI CTYRAG 3 cut(s) 540, 1088, 1818
SmoI CTYRAG 3 cut(s) 540, 1088, 1818
SpeI ACTAGT 1 cut(s) 2431
SphI GCATGC 1 cut(s) 2406
SseBI AGGCCT 1 cut(s) 2173
SsiI CCGC 5 cut(s) 123, 1248, 1524, 2088, 2263
SspMI CTAG 9 cut(s) 137, 1050, 1599, 1664, 1848, 1938, 2069, 2075, 2432
StuI AGGCCT 1 cut(s) 2173
StyD4I CCNGG 8 cut(s) 277, 442, 460, 906, 1064, 1152, 1932, 2344
StyI CCWWGG 1 cut(s) 629
TaiI ACGT 5 cut(s) 131, 177, 361, 1020, 1224
TaqI TCGA 6 cut(s) 332, 1611, 1755, 1819, 2379, 2443
TaqII GACCGA 3 cut(s) 595, 609, 2296
TatI WGTACW 5 cut(s) 77, 551, 717, 877, 1029
TauI GCSGC 1 cut(s) 1250
TfiI GAWTC 8 cut(s) 346, 368, 1323, 1660, 1844, 2106, 2236, 2266
Tru1I TTAA 7 cut(s) 612, 665, 767, 1071, 1542, 1806, 1908
Tru9I TTAA 7 cut(s) 612, 665, 767, 1071, 1542, 1806, 1908
TscAI CASTG 3 cut(s) 357, 655, 1581
TseFI GTSAC 4 cut(s) 268, 854, 2209, 2419
TseI GCWGC 7 cut(s) 31, 44, 787, 1250, 1402, 1683, 2260
Tsp45I GTSAC 4 cut(s) 268, 854, 2209, 2419
TspRI CASTG 3 cut(s) 357, 655, 1581
Tth111I GACNNNGTC 1 cut(s) 2207
Van91I CCANNNNNTGG 3 cut(s) 216, 635, 819
VpaK11BI GGWCC 4 cut(s) 318, 566, 626, 1532
VspI ATTAAT 1 cut(s) 1806
XagI CCTNNNNNAGG 1 cut(s) 540
XapI RAATTY 4 cut(s) 282, 614, 1141, 1904
XbaI TCTAGA 2 cut(s) 1598, 1847
XceI RCATGY 5 cut(s) 891, 2005, 2147, 2290, 2406
XhoI CTCGAG 1 cut(s) 1818
XmiI GTMKAC 1 cut(s) 1206
XmnI GAANNNNTTC 1 cut(s) 994
XspI CTAG 9 cut(s) 137, 1050, 1599, 1664, 1848, 1938, 2069, 2075, 2432
ZrmI AGTACT 1 cut(s) 1031
Zsp2I ATGCAT 3 cut(s) 868, 2149, 2194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.