Rw5G016870

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000484
Physical Location & Seq
Forward (+)
21510824 .. 21515136
4313 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G016870.1

Sequence Viewer

Length: 2361 bp
ATGACCCTTTTTGGATCAATGCTTGCCACAGCAGAAGCCAGCACAAGTACATTTCAGTCCATCAAAGACGGCGAGACCATAGTTTCAACTGATGGAACCTTTGAGCTCGGATTCTTCACTCCTGCAGGCGCTTCTGGTAATCGATACGTAGGCATATGGTACAAGAAGATATCGGTTACAACAGTTGTGTGGGTTGCCAACAGAGAAACACCCCTCACAGATTCATCAGGTATTCTCAAAATCACTAACCCTGGAATTCTTGTCCTTGTCAACCAGAACAAGACCACAGTTTGGTCCTCCAACACATCAACAACCGCACCGAATGCAGTGGCACAGCTTTTGGATTCAGGAAACCTTGTTGTGAAAGATGGCAGTGACAAAGTTGATCCAGAGAATGAGAAGTACTTGTGGCAGAGTTTTGACTACCCTGGTGATACATTGCTAGCAGGTATGAAGCTTGGTAGGAACACAGTTACAGGCTTCAATTGGCATCTTACATCATGGAGAAGTCCCTCTGATCCTTCTCAAGGCAACTTCACATTTCAACATGGTCCCAGGGGATATGCAGAACAAGTTCTGAGGGAGGGTTCAGACATAAGATTTCGGACTGGACCATGGAATGGAATCCGGTTCAGTGGAACGCCTCATTTAGGTCCAAACTATGTTTACACATATCAGCTAGTTTTTGATGATCATGATCATGAAGAATACTATAGCTATAAGCTTCTCAACAGCTCAGTTCTTTCCAGGCTGGTGTTAACTCAAGATGGGCTTCTGCAGCGCTACACATGGATCGATAGAACCCAAAGTTGGTTCCTTTACTTAACAGCACAGATGGATAACTGTGACAATTATGCACTTTGTGGTGCATATGGTGCTTGCAATATTGAGAACTCCCCAGTATGCGGCTGCTTGAAAGGTTTTGTGCCAAAATTTCCAAAAGATTGGGACACAATGGATTGGTCAAATGGGTGTGTGAGAAAGACTCCACTAGCTTGCAATGGCGACAAGTTTCTGAAGTACTCTGGGGTCAAATTTCCAAACACAGAACAATCCTGGTTTAACAAAAGTATGAGCCTCAAGGAATGCGAGACGGACGAAGGAGGAACTGGGTGCTTGATGTGGTTTGGCGATCTGATTGATATGAGAAATCTCACTGAAAATGGACAAGATATTTACATAAGGATGGCAGCATCAGAACAAGATCCTGAAGACCACACAAAGATCAATGGTAAACTTGCTAATTTCAATGTGAAGAAAAGGAGGATCATATTAAGCTGTATCGTGCCATCTACTGGACTGCTGATCCTGGGCCTAGCCTTTTGTCTATACATTTGCCAGAAGAAACATCAAAAAGATGGAAAACTGACGCACAGCAAGAAGGAAGATCTTGAATTACCATTGTTTGACTTGGCTACTATTGTCTCTGCAACCAGCAACTTTTCAAACGACAATAAGCTTGGAGAAGGTGGTTTTGGATGCGTCTTTAAGGGTATGTTGAGAAATGGGCAAGAAATAGCTGTCAAAAGGCTCTCAAAACATTCTTCACAAGGAGTAAATGAGTTCAAAAATGAGGTTACGCATATTGCCAAACTCCAGCACAGGAATCTAGTGAAGCTTCTTGGATGTTGCATTCAAGCCGATGAAATGATGCTCATCTACGAATGCATGCCTAACAAAAGCTTGGACTTCTTTATTTTTGATCAAAGAAAAAGCATGCTACTAGATTGGCCTAAGCGCTTTGACATCATCAATGGTATTGCTCGGGGGCTCCTCTATCTTCATCAAGATTCTAGACTGAGAGTAATTCATAGAGATCTCAAAGCAGGCAATATTTTATTAGACAATGAATTTAACCCCAAGATCTCAGACTTTGGTCTAGCTAGAATGGTTGGAACCTATGGTTACATGTCCCCAGAATATGCAAGTGACGGGTTCTACTCTACCAAATCCGACGTCTTCAGCTTTGGTGTTTCTTTGTTGGAGATAATAAGTGGGAAGAGAAATAGAGGATTCTCTCATCCAGACCACGACCTCAACCTTCTTGGACATGCATGGAATCTATACACGGAAGGCAGGTCCATAGAATTGCTTGATACATCAGTAGGGAACTCCACTAATCCATCTGAGGAAGTTCTGCGCTCAATTCATGTGGGTCTTTTATGTGTGCAGAAAGATCCAGAAGATAGGCCAAGCATGTCAGATGTTGTTCTAATGTTGAGTGGTGAAGGTACATTGCCTCAACCTGAAAAACCTGGGTTTTATAGTAAAAGTAGTGATAGGTCTGAAAACCAAGTCAATCCTTTATCTAGAGCATGCTCGGCTAATGAAGTTAGTTTTACGCTACTTGAGGCTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

786

Amino Acids

88.01

Weight (kDa)

6.14

Isoelectric Point (pI)

37.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 61 - 169 3.6e-38 D-mannose binding lectin
S_locus_glycop PF00954 201 - 312 3.1e-33 S-locus glycoprotein domain
Pkinase PF00069 482 - 737 3.4e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 484 - 738 9.2e-44 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1959
Acc36I ACCTGC 2 cut(s) 437, 2067
AccB7I CCANNNNNTGG 3 cut(s) 291, 620, 1295
AciI CCGC 2 cut(s) 315, 906
AclWI GGATC 8 cut(s) 22, 380, 512, 800, 1199, 1274, 1300, 2171
AcsI RAATTY 4 cut(s) 255, 932, 1034, 1850
AcuI CTGAAG 3 cut(s) 1037, 1230, 1945
AcyI GRCGYC 1 cut(s) 1956
AdeI CACNNNGTG 1 cut(s) 863
AfaI GTAC 5 cut(s) 49, 161, 404, 1022, 2233
AfeI AGCGCT 2 cut(s) 782, 1739
AfiI CCNNNNNNNGG 7 cut(s) 291, 527, 620, 650, 810, 905, 1295
AflIII ACRYGT 1 cut(s) 1908
AgsI TTSAA 9 cut(s) 87, 484, 545, 916, 1249, 1394, 1446, 1567, 1637
AjnI CCWGG 7 cut(s) 250, 427, 554, 746, 1055, 1308, 2254
AjuI GAANNNNNNNTTGG 4 cut(s) 793, 825, 1458, 1490
Alw21I GWGCWC 1 cut(s) 108
Alw26I GTCTC 3 cut(s) 68, 1085, 1429
AlwI GGATC 8 cut(s) 22, 380, 512, 800, 1199, 1274, 1300, 2171
Ama87I CYCGRG 1 cut(s) 1764
Aor51HI AGCGCT 2 cut(s) 782, 1739
AoxI GGCC 3 cut(s) 1312, 1730, 2189
ApeKI GCWGC 3 cut(s) 778, 909, 1190
ApoI RAATTY 4 cut(s) 255, 932, 1034, 1850
ArsI GACNNNNNNTTYG 2 cut(s) 1941, 1973
Asp700I GAANNNNTTC 1 cut(s) 573
AspLEI GCGC 4 cut(s) 131, 783, 1740, 2142
AspS9I GGNCC 6 cut(s) 294, 551, 611, 653, 1312, 2079
AsuHPI GGTGA 2 cut(s) 443, 2237
AsuNHI GCTAGC 1 cut(s) 442
AvaI CYCGRG 1 cut(s) 1764
AvaII GGWCC 5 cut(s) 294, 551, 611, 653, 2079
BaeI ACNNNNGTAYC 2 cut(s) 2088, 2121
BanII GRGCYC 2 cut(s) 108, 1773
BbsI GAAGAC 2 cut(s) 1218, 1951
Bbv12I GWGCWC 1 cut(s) 108
BbvI GCAGC 3 cut(s) 790, 896, 1202
BccI CCATC 9 cut(s) 68, 86, 362, 761, 829, 1180, 1297, 1352, 2131
BceAI ACGGC 1 cut(s) 85
BciT130I CCWGG 7 cut(s) 252, 429, 556, 748, 1057, 1310, 2256
BclI TGATCA 3 cut(s) 691, 697, 1702
BcoDI GTCTC 3 cut(s) 68, 1085, 1429
BfmI CTRYAG 3 cut(s) 123, 712, 776
BfoI RGCGCY 3 cut(s) 132, 784, 1741
BfuAI ACCTGC 2 cut(s) 437, 2067
BglII AGATCT 3 cut(s) 1387, 1816, 1863
BisI GCNGC 4 cut(s) 779, 907, 910, 1191
BlsI GCNGC 4 cut(s) 780, 908, 911, 1192
BmcAI AGTACT 2 cut(s) 404, 1022
Bme1390I CCNGG 7 cut(s) 252, 429, 556, 748, 1057, 1310, 2256
Bme18I GGWCC 5 cut(s) 294, 551, 611, 653, 2079
BmeT110I CYCGRG 1 cut(s) 1764
BmgT120I GGNCC 6 cut(s) 294, 551, 611, 653, 1312, 2079
BmiI GGNNCC 5 cut(s) 97, 553, 815, 1772, 1897
BmrFI CCNGG 7 cut(s) 252, 429, 556, 748, 1057, 1310, 2256
BmrI ACTGGG 2 cut(s) 893, 1119
BmsI GCATC 4 cut(s) 499, 1202, 1469, 1641
BmtI GCTAGC 1 cut(s) 446
BmuI ACTGGG 2 cut(s) 893, 1119
BoxI GACNNNNGTC 1 cut(s) 1875
BpiI GAAGAC 2 cut(s) 1218, 1951
BplI GAGNNNNNCTC 2 cut(s) 970, 1002
BpmI CTGGAG 1 cut(s) 1580
Bpu10I CCTNAGC 1 cut(s) 1734
BpuEI CTTGAG 3 cut(s) 510, 747, 1064
Bsa29I ATCGAT 2 cut(s) 142, 795
BsaAI YACGTR 1 cut(s) 148
BsaBI GATNNNNATC 2 cut(s) 696, 1655
BsaHI GRCGYC 1 cut(s) 1956
BsaI GGTCTC 1 cut(s) 68
BsaJI CCNNGG 7 cut(s) 250, 427, 554, 555, 614, 1309, 2255
BsaWI WCCGGW 1 cut(s) 627
Bsc4I CCNNNNNNNGG 7 cut(s) 291, 527, 620, 650, 810, 905, 1295
Bse1I ACTGG 4 cut(s) 613, 899, 1114, 1300
Bse3DI GCAATG 3 cut(s) 437, 1006, 2234
Bse8I GATNNNNATC 2 cut(s) 696, 1655
BseBI CCWGG 7 cut(s) 252, 429, 556, 748, 1057, 1310, 2256
BseCI ATCGAT 2 cut(s) 142, 795
BseDI CCNNGG 7 cut(s) 250, 427, 554, 555, 614, 1309, 2255
BseGI GGATG 4 cut(s) 1191, 1484, 1631, 2020
BseJI GATNNNNATC 2 cut(s) 696, 1655
BseLI CCNNNNNNNGG 7 cut(s) 291, 527, 620, 650, 810, 905, 1295
BseMI GCAATG 3 cut(s) 437, 1006, 2234
BseMII CTCAG 5 cut(s) 569, 750, 1790, 1881, 2118
BseNI ACTGG 4 cut(s) 613, 899, 1114, 1300
BseRI GAGGAG 1 cut(s) 1763
BseXI GCAGC 3 cut(s) 790, 896, 1202
BsgI GTGCAG 1 cut(s) 2189
BshFI GGCC 3 cut(s) 1314, 1732, 2191
BshVI ATCGAT 2 cut(s) 142, 795
BsiHKAI GWGCWC 1 cut(s) 108
BsiHKCI CYCGRG 1 cut(s) 1764
BsiSI CCGG 1 cut(s) 628
BslFI GGGAC 4 cut(s) 495, 537, 962, 1897
BslI CCNNNNNNNGG 7 cut(s) 291, 527, 620, 650, 810, 905, 1295
BsmAI GTCTC 3 cut(s) 68, 1085, 1429
BsmBI CGTCTC 1 cut(s) 1085
BsmFI GGGAC 4 cut(s) 495, 537, 962, 1897
BsmI GAATGC 4 cut(s) 328, 1091, 1632, 1670
BsnI GGCC 3 cut(s) 1314, 1732, 2191
Bso31I GGTCTC 1 cut(s) 68
BsoBI CYCGRG 1 cut(s) 1764
Bsp1286I GDGCHC 2 cut(s) 108, 1773
Bsp19I CCATGG 1 cut(s) 614
BspACI CCGC 2 cut(s) 315, 906
BspANI GGCC 3 cut(s) 1314, 1732, 2191
BspCNI CTCAG 5 cut(s) 570, 749, 1791, 1880, 2119
BspDI ATCGAT 2 cut(s) 142, 795
BspHI TCATGA 2 cut(s) 694, 700
BspLI GGNNCC 5 cut(s) 97, 553, 815, 1772, 1897
BspMAI CTGCAG 2 cut(s) 127, 780
BspMI ACCTGC 2 cut(s) 437, 2067
BspOI GCTAGC 1 cut(s) 446
BspPI GGATC 8 cut(s) 22, 380, 512, 800, 1199, 1274, 1300, 2171
BspTNI GGTCTC 1 cut(s) 68
BsrDI GCAATG 3 cut(s) 437, 1006, 2234
BsrI ACTGG 4 cut(s) 613, 899, 1114, 1300
BssECI CCNNGG 7 cut(s) 250, 427, 554, 555, 614, 1309, 2255
BssNI GRCGYC 1 cut(s) 1956
BssT1I CCWWGG 1 cut(s) 614
Bst2UI CCWGG 7 cut(s) 252, 429, 556, 748, 1057, 1310, 2256
Bst4CI ACNGT 4 cut(s) 184, 289, 472, 845
Bst6I CTCTTC 1 cut(s) 1994
BstACI GRCGYC 1 cut(s) 1956
BstAPI GCANNNNNTGC 2 cut(s) 323, 875
BstBAI YACGTR 1 cut(s) 148
BstDEI CTNAG 6 cut(s) 578, 736, 1734, 1799, 1867, 2127
BstDSI CCRYGG 1 cut(s) 614
BstENI CCTNNNNNAGG 2 cut(s) 525, 648
BstF5I GGATG 4 cut(s) 1191, 1484, 1631, 2020
BstH2I RGCGCY 3 cut(s) 132, 784, 1741
BstHHI GCGC 4 cut(s) 131, 783, 1740, 2142
BstMAI GTCTC 3 cut(s) 68, 1085, 1429
BstMWI GCNNNNNNNGC 4 cut(s) 323, 778, 875, 2321
BstNI CCWGG 7 cut(s) 252, 429, 556, 748, 1057, 1310, 2256
BstNSI RCATGY 6 cut(s) 1672, 1720, 1912, 2054, 2200, 2319
BstPAI GACNNNNGTC 1 cut(s) 1875
BstSCI CCNGG 7 cut(s) 250, 427, 554, 746, 1055, 1308, 2254
BstSFI CTRYAG 3 cut(s) 123, 712, 776
BstSNI TACGTA 1 cut(s) 148
BstV1I GCAGC 3 cut(s) 790, 896, 1202
BstV2I GAAGAC 2 cut(s) 1218, 1951
BstX2I RGATCY 5 cut(s) 1204, 1387, 1816, 1863, 2176
BstXI CCANNNNNNTGG 1 cut(s) 945
BstYI RGATCY 5 cut(s) 1204, 1387, 1816, 1863, 2176
Bsu15I ATCGAT 2 cut(s) 142, 795
BsuRI GGCC 3 cut(s) 1314, 1732, 2191
BsuTUI ATCGAT 2 cut(s) 142, 795
BtgI CCRYGG 1 cut(s) 614
BtsCI GGATG 4 cut(s) 1191, 1484, 1631, 2020
BtsI GCAGTG 2 cut(s) 333, 379
BtsIMutI CAGTG 4 cut(s) 333, 379, 640, 1155
BveI ACCTGC 2 cut(s) 437, 2067
CciI TCATGA 2 cut(s) 694, 700
CfoI GCGC 4 cut(s) 131, 783, 1740, 2142
Cfr13I GGNCC 6 cut(s) 294, 551, 611, 653, 1312, 2079
ClaI ATCGAT 2 cut(s) 142, 795
CseI GACGC 2 cut(s) 1378, 1471
Csp6I GTAC 5 cut(s) 48, 160, 403, 1021, 2232
CspCI CAANNNNNGTGG 2 cut(s) 2133, 2168
CviQI GTAC 5 cut(s) 48, 160, 403, 1021, 2232
DdeI CTNAG 6 cut(s) 578, 736, 1734, 1799, 1867, 2127
DraIII CACNNNGTG 1 cut(s) 863
Eam1104I CTCTTC 1 cut(s) 1994
EarI CTCTTC 1 cut(s) 1994
Ecl136II GAGCTC 1 cut(s) 106
Eco105I TACGTA 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 614
Eco24I GRGCYC 2 cut(s) 108, 1773
Eco31I GGTCTC 1 cut(s) 68
Eco32I GATATC 1 cut(s) 171
Eco47I GGWCC 5 cut(s) 294, 551, 611, 653, 2079
Eco47III AGCGCT 2 cut(s) 782, 1739
Eco53kI GAGCTC 1 cut(s) 106
Eco57I CTGAAG 3 cut(s) 1037, 1230, 1945
Eco88I CYCGRG 1 cut(s) 1764
EcoICRI GAGCTC 1 cut(s) 106
EcoNI CCTNNNNNAGG 2 cut(s) 525, 648
EcoRI GAATTC 1 cut(s) 255
EcoRII CCWGG 7 cut(s) 250, 427, 554, 746, 1055, 1308, 2254
EcoRV GATATC 1 cut(s) 171
EcoT14I CCWWGG 1 cut(s) 614
EcoT22I ATGCAT 2 cut(s) 1670, 2056
EcoT38I GRGCYC 2 cut(s) 108, 1773
ErhI CCWWGG 1 cut(s) 614
Esp3I CGTCTC 1 cut(s) 1085
FalI AAGNNNNNCTT 2 cut(s) 756, 788
FaqI GGGAC 4 cut(s) 495, 537, 962, 1897
FauNDI CATATG 2 cut(s) 155, 871
FbaI TGATCA 3 cut(s) 691, 697, 1702
Fnu4HI GCNGC 4 cut(s) 779, 907, 910, 1191
FokI GGATG 4 cut(s) 1198, 1491, 1638, 2007
FriOI GRGCYC 2 cut(s) 108, 1773
Fsp4HI GCNGC 4 cut(s) 779, 907, 910, 1191
GlaI GCGC 4 cut(s) 130, 782, 1739, 2141
GluI GCNGC 4 cut(s) 779, 907, 910, 1191
GsuI CTGGAG 1 cut(s) 1580
HaeII RGCGCY 3 cut(s) 132, 784, 1741
HaeIII GGCC 3 cut(s) 1314, 1732, 2191
HapII CCGG 1 cut(s) 628
HgaI GACGC 2 cut(s) 1378, 1471
HhaI GCGC 4 cut(s) 131, 783, 1740, 2142
Hin1I GRCGYC 1 cut(s) 1956
Hin6I GCGC 4 cut(s) 129, 781, 1738, 2140
HinP1I GCGC 4 cut(s) 129, 781, 1738, 2140
HincII GTYRAC 2 cut(s) 271, 759
HindII GTYRAC 2 cut(s) 271, 759
HindIII AAGCTT 5 cut(s) 455, 722, 1457, 1616, 1681
HinfI GANTC 9 cut(s) 111, 221, 344, 624, 985, 1606, 1790, 2013, 2059
HpaI GTTAAC 1 cut(s) 759
HpaII CCGG 1 cut(s) 628
HphI GGTGA 2 cut(s) 443, 2237
Hpy166II GTNNAC 4 cut(s) 271, 667, 759, 1235
Hpy8I GTNNAC 4 cut(s) 271, 667, 759, 1235
Hpy99I CGWCG 1 cut(s) 1958
HpyAV CCTTC 7 cut(s) 531, 1094, 1375, 1460, 2051, 2066, 2222
HpyCH4III ACNGT 4 cut(s) 184, 289, 472, 845
HpyCH4IV ACGT 2 cut(s) 147, 1956
HpyF10VI GCNNNNNNNGC 4 cut(s) 323, 778, 875, 2321
HpyF3I CTNAG 6 cut(s) 578, 736, 1734, 1799, 1867, 2127
HpySE526I ACGT 2 cut(s) 147, 1956
Hsp92I GRCGYC 1 cut(s) 1956
HspAI GCGC 4 cut(s) 129, 781, 1738, 2140
Ksp22I TGATCA 3 cut(s) 691, 697, 1702
KspAI GTTAAC 1 cut(s) 759
LmnI GCTCC 1 cut(s) 1776
Lsp1109I GCAGC 3 cut(s) 790, 896, 1202
LweI GCATC 4 cut(s) 499, 1202, 1469, 1641
MaeII ACGT 2 cut(s) 147, 1956
MaeIII GTNAC 7 cut(s) 175, 374, 472, 845, 1576, 1904, 1928
MfeI CAATTG 1 cut(s) 484
MflI RGATCY 5 cut(s) 1204, 1387, 1816, 1863, 2176
MhlI GDGCHC 2 cut(s) 108, 1773
MlyI GAGTC 1 cut(s) 979
MmeI TCCRAC 4 cut(s) 324, 1873, 1962, 1977
Mph1103I ATGCAT 2 cut(s) 1670, 2056
MroXI GAANNNNTTC 1 cut(s) 573
MseI TTAA 6 cut(s) 758, 824, 1062, 1274, 1488, 1854
MslI CAYNNNNRTG 2 cut(s) 699, 1184
MspI CCGG 1 cut(s) 628
MspR9I CCNGG 7 cut(s) 252, 429, 556, 748, 1057, 1310, 2256
MunI CAATTG 1 cut(s) 484
Mva1269I GAATGC 4 cut(s) 328, 1091, 1632, 1670
MvaI CCWGG 7 cut(s) 252, 429, 556, 748, 1057, 1310, 2256
MwoI GCNNNNNNNGC 4 cut(s) 323, 778, 875, 2321
NcoI CCATGG 1 cut(s) 614
NdeI CATATG 2 cut(s) 155, 871
NheI GCTAGC 1 cut(s) 442
NlaIV GGNNCC 5 cut(s) 97, 553, 815, 1772, 1897
NmeAIII GCCGAG 1 cut(s) 2300
NmuCI GTSAC 3 cut(s) 374, 845, 1928
NsiI ATGCAT 2 cut(s) 1670, 2056
NspI RCATGY 6 cut(s) 1672, 1720, 1912, 2054, 2200, 2319
PaeI GCATGC 3 cut(s) 1672, 1720, 2319
PagI TCATGA 2 cut(s) 694, 700
PasI CCCWGGG 1 cut(s) 555
PciI ACATGT 1 cut(s) 1908
PctI GAATGC 4 cut(s) 328, 1091, 1632, 1670
PdmI GAANNNNTTC 1 cut(s) 573
PfeI GAWTC 8 cut(s) 111, 221, 344, 624, 1606, 1790, 2013, 2059
PflMI CCANNNNNTGG 3 cut(s) 291, 620, 1295
PkrI GCNGC 4 cut(s) 780, 908, 911, 1192
PleI GAGTC 1 cut(s) 979
PpsI GAGTC 1 cut(s) 979
Ppu21I YACGTR 1 cut(s) 148
PscI ACATGT 1 cut(s) 1908
PshAI GACNNNNGTC 1 cut(s) 1875
Psp124BI GAGCTC 1 cut(s) 108
Psp6I CCWGG 7 cut(s) 250, 427, 554, 746, 1055, 1308, 2254
PspGI CCWGG 7 cut(s) 250, 427, 554, 746, 1055, 1308, 2254
PspN4I GGNNCC 5 cut(s) 97, 553, 815, 1772, 1897
PspPI GGNCC 6 cut(s) 294, 551, 611, 653, 1312, 2079
PstI CTGCAG 2 cut(s) 127, 780
PsuI RGATCY 5 cut(s) 1204, 1387, 1816, 1863, 2176
RsaI GTAC 5 cut(s) 49, 161, 404, 1022, 2233
RsaNI GTAC 5 cut(s) 48, 160, 403, 1021, 2232
RseI CAYNNNNRTG 2 cut(s) 699, 1184
SacI GAGCTC 1 cut(s) 108
SaqAI TTAA 6 cut(s) 758, 824, 1062, 1274, 1488, 1854
SatI GCNGC 4 cut(s) 779, 907, 910, 1191
Sau96I GGNCC 6 cut(s) 294, 551, 611, 653, 1312, 2079
SbfI CCTGCAGG 1 cut(s) 127
ScaI AGTACT 2 cut(s) 404, 1022
SchI GAGTC 1 cut(s) 979
ScrFI CCNGG 7 cut(s) 252, 429, 556, 748, 1057, 1310, 2256
SdaI CCTGCAGG 1 cut(s) 127
SduI GDGCHC 2 cut(s) 108, 1773
SfaNI GCATC 4 cut(s) 499, 1202, 1469, 1641
SfcI CTRYAG 3 cut(s) 123, 712, 776
SinI GGWCC 5 cut(s) 294, 551, 611, 653, 2079
SmiMI CAYNNNNRTG 2 cut(s) 699, 1184
SmlI CTYRAG 4 cut(s) 525, 762, 1079, 2348
SmoI CTYRAG 4 cut(s) 525, 762, 1079, 2348
SnaBI TACGTA 1 cut(s) 148
SphI GCATGC 3 cut(s) 1672, 1720, 2319
Sse8387I CCTGCAGG 1 cut(s) 127
SsiI CCGC 2 cut(s) 315, 906
SspI AATATT 2 cut(s) 886, 1834
SstI GAGCTC 1 cut(s) 108
StyD4I CCNGG 7 cut(s) 250, 427, 554, 746, 1055, 1308, 2254
StyI CCWWGG 1 cut(s) 614
TaaI ACNGT 4 cut(s) 184, 289, 472, 845
TaiI ACGT 2 cut(s) 150, 1959
TaqI TCGA 2 cut(s) 142, 795
TatI WGTACW 3 cut(s) 47, 402, 1020
TauI GCSGC 1 cut(s) 909
TfiI GAWTC 8 cut(s) 111, 221, 344, 624, 1606, 1790, 2013, 2059
Tru1I TTAA 6 cut(s) 758, 824, 1062, 1274, 1488, 1854
Tru9I TTAA 6 cut(s) 758, 824, 1062, 1274, 1488, 1854
TscAI CASTG 4 cut(s) 333, 379, 640, 1162
TseFI GTSAC 3 cut(s) 374, 845, 1928
TseI GCWGC 3 cut(s) 778, 909, 1190
Tsp45I GTSAC 3 cut(s) 374, 845, 1928
TspDTI ATGAA 9 cut(s) 213, 467, 717, 1659, 1772, 1799, 1863, 2138, 2343
TspGWI ACGGA 2 cut(s) 1109, 2084
TspRI CASTG 4 cut(s) 333, 379, 640, 1162
Van91I CCANNNNNTGG 3 cut(s) 291, 620, 1295
VpaK11BI GGWCC 5 cut(s) 294, 551, 611, 653, 2079
XagI CCTNNNNNAGG 2 cut(s) 525, 648
XapI RAATTY 4 cut(s) 255, 932, 1034, 1850
XbaI TCTAGA 2 cut(s) 1793, 2309
XceI RCATGY 6 cut(s) 1672, 1720, 1912, 2054, 2200, 2319
XmnI GAANNNNTTC 1 cut(s) 573
ZraI GACGTC 1 cut(s) 1957
ZrmI AGTACT 2 cut(s) 404, 1022
Zsp2I ATGCAT 2 cut(s) 1670, 2056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.