Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5C

Orthogroup ID

OG0000484

Physical Location & Seq

Genomic Coordinates Forward (+)

20323952 .. 20352915

Gene Structure 28964 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5CG202200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1797 bp

ATGTGCTTGCCTGGAGGTGGTGATCAGCAAGGGACTGCAACGCTTGCCACAGCAGAAGCCAGCACAAGTACATTTCAGTCCATCAAAGACGGCGAGACCATAGTTTCAACTGATGGAACCTTTGAGCTCGGATTCTTCACTCCTGCAGGCGCTTCTGGTAATCGATACGTAGGCATATGGTACAAGAAGATATCGGTTACAACAGTTGTGTGGGTTGCCAACAGAGAAACACCCCTCACAGATTCATCAGGTATTCTCAAAATCACTAACCCTGGAATTCTTGTCCTTGTCAACCAGAACAAGACCACAGTTTGGTCCTCCAACACATCAACAACCGCACCGAATGCAGTGGCACAGCTTTTGGATTCAGGAAACCTTGTTGTGAAAGATGGCAGTGACAAAGTTGATGCAGAGGATGAGAAGTACTTGTGGCAGAGTTTTGACTACCCGGGTGATACATTGCTAGCAGGTATGAAGCTTGGTAGGAACACAGTTACAGGCTTCAATTGGCATCTTACATCATGGAGAAGTCCCTCTGATCCTTCTCAAGGCAACTTCACATTTCAACATGGTCCCAGGGGATATGCAGAACAAGTTCTGAGGGAGGGTTCAGACATAACATTTCGGACTGGACCATGGAATGGAATCCGGTTCAGTGGAACGCCTCATTTAGGTCCAAACTATGTTTACACATATCAGCTAGTTTTTGATGATCATGATCATGAAGAATACTATAGCTATAAGCTTCTCAACAGCTCAGTTCTTTCCAGGCTGGTGTTAACTCAAGATGGGCTTCTGCAGCGCTACACATGGATCGATAGAACCCAAAGTTGGTTTCTTTACTTAACAGCACAGATGGATAACTGTGACAATTATGCACTTTGTGGTGCATATGGTGCTTGCAATATTGAGAACTCCCCAGTATGCGGCTGCTTGAAAGGTTTTGTGCCAAAATTTCCAAAAGATTGGGACACAATGGATTGGTCAAATGGGTGTGTGAGAAAGACTCCACTAGCTTGCAATGGCGACAAGTTTCTGAAGTACTCTGGGGTCAAATTTCCAAACACAGAACAATCCTGGTTTAACAAAAGTATGAGCCTCAAGGAATGCGAGATGCAGTGCACCAGAAACTGTTCCTGCACAGCTTATTCAAATTTGTATATTAGCGAAGGAGGAACTGGGTGCTTGATGTGGTTTGGCGATCTGATTGATATGAGAAATCTCACTGAAAATGGACAAGATATATACATAAGGATGGCAGCATCAGAACAAGATCCTGAAGACTATACAAAGATCAATGGTAAACTTGCTAATTTCAATGTGAAGAAAAGGAGGATCATATTAAGCTGTATCGTGCCATCTACTGGACTGCTGATCCTGGGCCTAGCCTTCTGTCTATACATTTTCCAGAAGAAACATAAAAAAGATGGTAAATTGTTTGCATGGAATCTATACACAGAAGGCAGGTCCATAGAATTGCTTGATACATCAGTAGGGGACTCCACTAATCCATCCGAGGAAGTTCTGCGCTCGATTCATGTGGGTCTTTTATGTGTGCAGAAAGATCCAGAAGATAGGCCAAGCATGTCAGATGTTGTTCGAATGTTGAGTGGTGAAGGTGCATTGTCTCAACCTGAAAAACCTGGGTTTTATAGTAAAAGTAGTGATAGGTCTGAAAACCAAGTCAATCATTTATCTAGAGCATGCTCGGCTAATGAAGCTCCTTTGAATTCTCAGTTAGCTTTTAGGTCAAAGTTGGGGCCAAAACCCTCACGCATGTGGGACACTCTCATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

598

Amino Acids

66.55

Weight (kDa)

5.87

Isoelectric Point (pI)

41.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
B_lectin	PF01453	68 - 176	1.7e-38	D-mannose binding lectin
S_locus_glycop	PF00954	208 - 319	2e-33	S-locus glycoprotein domain
PAN_2	PF08276	340 - 405	3.1e-20	PAN-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000484)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27290 AT4G27290
fragaria_vesca	FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica	MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica	Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis	pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis	RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata	RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora	Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii	Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa	Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha	Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana	Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	458, 1455
AccB7I	CCANNNNNTGG	3 cut(s)	312, 641, 1364
AciI	CCGC	2 cut(s)	336, 927
AclWI	GGATC	6 cut(s)	533, 821, 1268, 1343, 1369, 1559
AcsI	RAATTY	5 cut(s)	276, 953, 1055, 1153, 1729
AcuI	CTGAAG	2 cut(s)	1058, 1299
AdeI	CACNNNGTG	1 cut(s)	884
AfaI	GTAC	4 cut(s)	70, 182, 425, 1043
AfeI	AGCGCT	1 cut(s)	803
AfiI	CCNNNNNNNGG	8 cut(s)	17, 312, 548, 641, 671, 831, 926, 1364
AgsI	TTSAA	7 cut(s)	108, 505, 566, 937, 1152, 1318, 1729
AjnI	CCWGG	7 cut(s)	10, 271, 575, 767, 1076, 1377, 1642
AjuI	GAANNNNNNNTTGG	4 cut(s)	814, 819, 846, 851
AleI	CACNNNNGTG	1 cut(s)	1777
Alw21I	GWGCWC	2 cut(s)	129, 1124
Alw26I	GTCTC	2 cut(s)	89, 1632
Alw44I	GTGCAC	1 cut(s)	1120
AlwI	GGATC	6 cut(s)	533, 821, 1268, 1343, 1369, 1559
AlwNI	CAGNNNCTG	1 cut(s)	1131
Ama87I	CYCGRG	1 cut(s)	448
Aor51HI	AGCGCT	1 cut(s)	803
AoxI	GGCC	3 cut(s)	1381, 1577, 1760
ApaLI	GTGCAC	1 cut(s)	1120
ApeKI	GCWGC	3 cut(s)	799, 930, 1259
ApoI	RAATTY	5 cut(s)	276, 953, 1055, 1153, 1729
Asp700I	GAANNNNTTC	2 cut(s)	594, 1132
AspLEI	GCGC	3 cut(s)	152, 804, 1530
AspS9I	GGNCC	7 cut(s)	315, 572, 632, 674, 1381, 1467, 1760
AsuC2I	CCSGG	2 cut(s)	449, 450
AsuHPI	GGTGA	3 cut(s)	32, 464, 1625
AsuII	TTCGAA	1 cut(s)	1600
AsuNHI	GCTAGC	1 cut(s)	463
AvaI	CYCGRG	1 cut(s)	448
AvaII	GGWCC	5 cut(s)	315, 572, 632, 674, 1467
BaeGI	GKGCMC	1 cut(s)	1124
BaeI	ACNNNNGTAYC	2 cut(s)	1476, 1509
BanII	GRGCYC	1 cut(s)	129
BbsI	GAAGAC	1 cut(s)	1287
Bbv12I	GWGCWC	2 cut(s)	129, 1124
BbvI	GCAGC	3 cut(s)	811, 917, 1271
BccI	CCATC	9 cut(s)	89, 107, 383, 782, 850, 1249, 1366, 1421, 1519
BceAI	ACGGC	1 cut(s)	106
BciT130I	CCWGG	7 cut(s)	12, 273, 577, 769, 1078, 1379, 1644
BclI	TGATCA	3 cut(s)	22, 712, 718
BcnI	CCSGG	2 cut(s)	449, 450
BcoDI	GTCTC	2 cut(s)	89, 1632
BfaI	CTAG	5 cut(s)	464, 701, 1013, 1385, 1698
BfmI	CTRYAG	3 cut(s)	144, 733, 797
BfoI	RGCGCY	2 cut(s)	153, 805
BfuAI	ACCTGC	2 cut(s)	458, 1455
BisI	GCNGC	4 cut(s)	800, 928, 931, 1260
BlsI	GCNGC	4 cut(s)	801, 929, 932, 1261
BmcAI	AGTACT	2 cut(s)	425, 1043
Bme1390I	CCNGG	9 cut(s)	12, 273, 449, 450, 577, 769, 1078, 1379, 1644
Bme18I	GGWCC	5 cut(s)	315, 572, 632, 674, 1467
BmeT110I	CYCGRG	1 cut(s)	448
BmgT120I	GGNCC	7 cut(s)	315, 572, 632, 674, 1381, 1467, 1760
BmiI	GGNNCC	3 cut(s)	118, 574, 1761
BmrFI	CCNGG	9 cut(s)	12, 273, 449, 450, 577, 769, 1078, 1379, 1644
BmrI	ACTGGG	2 cut(s)	914, 1188
BmsI	GCATC	4 cut(s)	397, 520, 1104, 1271
BmtI	GCTAGC	1 cut(s)	467
BmuI	ACTGGG	2 cut(s)	914, 1188
BpiI	GAAGAC	1 cut(s)	1287
BplI	GAGNNNNNCTC	2 cut(s)	991, 1023
BpmI	CTGGAG	1 cut(s)	33
Bpu14I	TTCGAA	1 cut(s)	1600
BpuEI	CTTGAG	3 cut(s)	531, 768, 1085
BpuMI	CCSGG	2 cut(s)	449, 450
Bsa29I	ATCGAT	2 cut(s)	163, 816
BsaAI	YACGTR	1 cut(s)	169
BsaBI	GATNNNNATC	1 cut(s)	717
BsaI	GGTCTC	1 cut(s)	89
BsaJI	CCNNGG	8 cut(s)	271, 448, 575, 576, 635, 1378, 1515, 1643
BsaWI	WCCGGW	1 cut(s)	648
Bsc4I	CCNNNNNNNGG	8 cut(s)	17, 312, 548, 641, 671, 831, 926, 1364
Bse1I	ACTGG	4 cut(s)	634, 920, 1183, 1369
Bse3DI	GCAATG	2 cut(s)	458, 1027
Bse8I	GATNNNNATC	1 cut(s)	717
BseBI	CCWGG	7 cut(s)	12, 273, 577, 769, 1078, 1379, 1644
BseCI	ATCGAT	2 cut(s)	163, 816
BseDI	CCNNGG	8 cut(s)	271, 448, 575, 576, 635, 1378, 1515, 1643
BseGI	GGATG	3 cut(s)	421, 1260, 1511
BseJI	GATNNNNATC	1 cut(s)	717
BseLI	CCNNNNNNNGG	8 cut(s)	17, 312, 548, 641, 671, 831, 926, 1364
BseMI	GCAATG	2 cut(s)	458, 1027
BseMII	CTCAG	3 cut(s)	590, 771, 1748
BseNI	ACTGG	4 cut(s)	634, 920, 1183, 1369
BseSI	GKGCMC	1 cut(s)	1124
BseXI	GCAGC	3 cut(s)	811, 917, 1271
BsgI	GTGCAG	2 cut(s)	1123, 1577
BshFI	GGCC	3 cut(s)	1383, 1579, 1762
BshVI	ATCGAT	2 cut(s)	163, 816
BsiHKAI	GWGCWC	2 cut(s)	129, 1124
BsiHKCI	CYCGRG	1 cut(s)	448
BsiSI	CCGG	2 cut(s)	449, 649
BslFI	GGGAC	5 cut(s)	46, 516, 558, 983, 1511
BslI	CCNNNNNNNGG	8 cut(s)	17, 312, 548, 641, 671, 831, 926, 1364
BsmAI	GTCTC	2 cut(s)	89, 1632
BsmFI	GGGAC	5 cut(s)	46, 516, 558, 983, 1511
BsmI	GAATGC	2 cut(s)	349, 1112
BsnI	GGCC	3 cut(s)	1383, 1579, 1762
Bso31I	GGTCTC	1 cut(s)	89
BsoBI	CYCGRG	1 cut(s)	448
Bsp119I	TTCGAA	1 cut(s)	1600
Bsp1286I	GDGCHC	2 cut(s)	129, 1124
Bsp19I	CCATGG	1 cut(s)	635
BspACI	CCGC	2 cut(s)	336, 927
BspANI	GGCC	3 cut(s)	1383, 1579, 1762
BspCNI	CTCAG	3 cut(s)	591, 770, 1747
BspDI	ATCGAT	2 cut(s)	163, 816
BspHI	TCATGA	2 cut(s)	715, 721
BspLI	GGNNCC	3 cut(s)	118, 574, 1761
BspMAI	CTGCAG	2 cut(s)	148, 801
BspMI	ACCTGC	2 cut(s)	458, 1455
BspOI	GCTAGC	1 cut(s)	467
BspPI	GGATC	6 cut(s)	533, 821, 1268, 1343, 1369, 1559
BspT104I	TTCGAA	1 cut(s)	1600
BspTNI	GGTCTC	1 cut(s)	89
BsrDI	GCAATG	2 cut(s)	458, 1027
BsrI	ACTGG	4 cut(s)	634, 920, 1183, 1369
BssECI	CCNNGG	8 cut(s)	271, 448, 575, 576, 635, 1378, 1515, 1643
BssT1I	CCWWGG	1 cut(s)	635
Bst2UI	CCWGG	7 cut(s)	12, 273, 577, 769, 1078, 1379, 1644
Bst4CI	ACNGT	5 cut(s)	205, 310, 493, 866, 1133
BstAPI	GCANNNNNTGC	3 cut(s)	44, 344, 896
BstBAI	YACGTR	1 cut(s)	169
BstBI	TTCGAA	1 cut(s)	1600
BstC8I	GCNNGC	8 cut(s)	8, 45, 61, 148, 465, 901, 1018, 1705
BstDEI	CTNAG	3 cut(s)	599, 757, 1734
BstDSI	CCRYGG	1 cut(s)	635
BstENI	CCTNNNNNAGG	2 cut(s)	546, 669
BstF5I	GGATG	3 cut(s)	421, 1260, 1511
BstH2I	RGCGCY	2 cut(s)	153, 805
BstHHI	GCGC	3 cut(s)	152, 804, 1530
BstMAI	GTCTC	2 cut(s)	89, 1632
BstMWI	GCNNNNNNNGC	6 cut(s)	44, 344, 799, 896, 1709, 1718
BstNI	CCWGG	7 cut(s)	12, 273, 577, 769, 1078, 1379, 1644
BstNSI	RCATGY	3 cut(s)	1588, 1707, 1780
BstSCI	CCNGG	9 cut(s)	10, 271, 447, 448, 575, 767, 1076, 1377, 1642
BstSFI	CTRYAG	3 cut(s)	144, 733, 797
BstSLI	GKGCMC	1 cut(s)	1124
BstSNI	TACGTA	1 cut(s)	169
BstV1I	GCAGC	3 cut(s)	811, 917, 1271
BstV2I	GAAGAC	1 cut(s)	1287
BstX2I	RGATCY	2 cut(s)	1273, 1564
BstXI	CCANNNNNNTGG	1 cut(s)	966
BstYI	RGATCY	2 cut(s)	1273, 1564
Bsu15I	ATCGAT	2 cut(s)	163, 816
BsuRI	GGCC	3 cut(s)	1383, 1579, 1762
BsuTUI	ATCGAT	2 cut(s)	163, 816
BtgI	CCRYGG	1 cut(s)	635
BtsCI	GGATG	3 cut(s)	421, 1260, 1511
BtsI	GCAGTG	3 cut(s)	354, 400, 1124
BtsIMutI	CAGTG	5 cut(s)	354, 400, 661, 1124, 1224
BveI	ACCTGC	2 cut(s)	458, 1455
Cac8I	GCNNGC	8 cut(s)	8, 45, 61, 148, 465, 901, 1018, 1705
CaiI	CAGNNNCTG	1 cut(s)	1131
CciI	TCATGA	2 cut(s)	715, 721
CfoI	GCGC	3 cut(s)	152, 804, 1530
Cfr13I	GGNCC	7 cut(s)	315, 572, 632, 674, 1381, 1467, 1760
Cfr9I	CCCGGG	1 cut(s)	448
ClaI	ATCGAT	2 cut(s)	163, 816
Csp6I	GTAC	4 cut(s)	69, 181, 424, 1042
CviQI	GTAC	4 cut(s)	69, 181, 424, 1042
DdeI	CTNAG	3 cut(s)	599, 757, 1734
DraIII	CACNNNGTG	1 cut(s)	884
Ecl136II	GAGCTC	1 cut(s)	127
Eco105I	TACGTA	1 cut(s)	169
Eco130I	CCWWGG	1 cut(s)	635
Eco24I	GRGCYC	1 cut(s)	129
Eco31I	GGTCTC	1 cut(s)	89
Eco32I	GATATC	1 cut(s)	192
Eco47I	GGWCC	5 cut(s)	315, 572, 632, 674, 1467
Eco47III	AGCGCT	1 cut(s)	803
Eco53kI	GAGCTC	1 cut(s)	127
Eco57I	CTGAAG	2 cut(s)	1058, 1299
Eco88I	CYCGRG	1 cut(s)	448
EcoICRI	GAGCTC	1 cut(s)	127
EcoNI	CCTNNNNNAGG	2 cut(s)	546, 669
EcoRI	GAATTC	2 cut(s)	276, 1729
EcoRII	CCWGG	7 cut(s)	10, 271, 575, 767, 1076, 1377, 1642
EcoRV	GATATC	1 cut(s)	192
EcoT14I	CCWWGG	1 cut(s)	635
EcoT38I	GRGCYC	1 cut(s)	129
ErhI	CCWWGG	1 cut(s)	635
FalI	AAGNNNNNCTT	2 cut(s)	777, 809
FaqI	GGGAC	5 cut(s)	46, 516, 558, 983, 1511
FauNDI	CATATG	2 cut(s)	176, 892
FbaI	TGATCA	3 cut(s)	22, 712, 718
Fnu4HI	GCNGC	4 cut(s)	800, 928, 931, 1260
FokI	GGATG	3 cut(s)	428, 1267, 1498
FriOI	GRGCYC	1 cut(s)	129
Fsp4HI	GCNGC	4 cut(s)	800, 928, 931, 1260
FspBI	CTAG	5 cut(s)	464, 701, 1013, 1385, 1698
GlaI	GCGC	3 cut(s)	151, 803, 1529
GluI	GCNGC	4 cut(s)	800, 928, 931, 1260
GsuI	CTGGAG	1 cut(s)	33
HaeII	RGCGCY	2 cut(s)	153, 805
HaeIII	GGCC	3 cut(s)	1383, 1579, 1762
HapII	CCGG	2 cut(s)	449, 649
HhaI	GCGC	3 cut(s)	152, 804, 1530
Hin6I	GCGC	3 cut(s)	150, 802, 1528
HinP1I	GCGC	3 cut(s)	150, 802, 1528
HincII	GTYRAC	2 cut(s)	292, 780
HindII	GTYRAC	2 cut(s)	292, 780
HindIII	AAGCTT	2 cut(s)	476, 743
HinfI	GANTC	8 cut(s)	132, 242, 365, 645, 1006, 1447, 1499, 1534
HpaI	GTTAAC	1 cut(s)	780
HpaII	CCGG	2 cut(s)	449, 649
HphI	GGTGA	3 cut(s)	32, 464, 1625
Hpy166II	GTNNAC	5 cut(s)	292, 688, 780, 1122, 1304
Hpy188III	TCNNGA	8 cut(s)	369, 716, 722, 785, 1277, 1408, 1568, 1698
Hpy8I	GTNNAC	5 cut(s)	292, 688, 780, 1122, 1304
HpyAV	CCTTC	5 cut(s)	552, 1163, 1399, 1454, 1610
HpyCH4III	ACNGT	5 cut(s)	205, 310, 493, 866, 1133
HpyCH4IV	ACGT	1 cut(s)	168
HpyF10VI	GCNNNNNNNGC	6 cut(s)	44, 344, 799, 896, 1709, 1718
HpyF3I	CTNAG	3 cut(s)	599, 757, 1734
HpySE526I	ACGT	1 cut(s)	168
HspAI	GCGC	3 cut(s)	150, 802, 1528
Ksp22I	TGATCA	3 cut(s)	22, 712, 718
KspAI	GTTAAC	1 cut(s)	780
LmnI	GCTCC	1 cut(s)	1726
Lsp1109I	GCAGC	3 cut(s)	811, 917, 1271
LweI	GCATC	4 cut(s)	397, 520, 1104, 1271
MaeI	CTAG	5 cut(s)	464, 701, 1013, 1385, 1698
MaeII	ACGT	1 cut(s)	168
MaeIII	GTNAC	4 cut(s)	196, 395, 493, 866
MboII	GAAGA	7 cut(s)	127, 199, 737, 1292, 1336, 1423, 1583
MfeI	CAATTG	1 cut(s)	505
MflI	RGATCY	2 cut(s)	1273, 1564
MhlI	GDGCHC	2 cut(s)	129, 1124
MlyI	GAGTC	2 cut(s)	1000, 1493
MmeI	TCCRAC	1 cut(s)	345
MroXI	GAANNNNTTC	2 cut(s)	594, 1132
MseI	TTAA	5 cut(s)	779, 845, 1083, 1343, 1795
MslI	CAYNNNNRTG	3 cut(s)	720, 1253, 1777
MspI	CCGG	2 cut(s)	449, 649
MspR9I	CCNGG	9 cut(s)	12, 273, 449, 450, 577, 769, 1078, 1379, 1644
MunI	CAATTG	1 cut(s)	505
Mva1269I	GAATGC	2 cut(s)	349, 1112
MvaI	CCWGG	7 cut(s)	12, 273, 577, 769, 1078, 1379, 1644
MwoI	GCNNNNNNNGC	6 cut(s)	44, 344, 799, 896, 1709, 1718
NciI	CCSGG	2 cut(s)	449, 450
NcoI	CCATGG	1 cut(s)	635
NdeI	CATATG	2 cut(s)	176, 892
NheI	GCTAGC	1 cut(s)	463
NlaIV	GGNNCC	3 cut(s)	118, 574, 1761
NmeAIII	GCCGAG	1 cut(s)	1688
NmuCI	GTSAC	2 cut(s)	395, 866
NspI	RCATGY	3 cut(s)	1588, 1707, 1780
NspV	TTCGAA	1 cut(s)	1600
OliI	CACNNNNGTG	1 cut(s)	1777
PaeI	GCATGC	1 cut(s)	1707
PagI	TCATGA	2 cut(s)	715, 721
PasI	CCCWGGG	1 cut(s)	576
PctI	GAATGC	2 cut(s)	349, 1112
PdmI	GAANNNNTTC	2 cut(s)	594, 1132
PfeI	GAWTC	6 cut(s)	132, 242, 365, 645, 1447, 1534
PflMI	CCANNNNNTGG	3 cut(s)	312, 641, 1364
PkrI	GCNGC	4 cut(s)	801, 929, 932, 1261
PleI	GAGTC	2 cut(s)	1000, 1493
PpsI	GAGTC	2 cut(s)	1000, 1493
Ppu21I	YACGTR	1 cut(s)	169
Psp124BI	GAGCTC	1 cut(s)	129
Psp6I	CCWGG	7 cut(s)	10, 271, 575, 767, 1076, 1377, 1642
PspGI	CCWGG	7 cut(s)	10, 271, 575, 767, 1076, 1377, 1642
PspN4I	GGNNCC	3 cut(s)	118, 574, 1761
PspPI	GGNCC	7 cut(s)	315, 572, 632, 674, 1381, 1467, 1760
PstI	CTGCAG	2 cut(s)	148, 801
PstNI	CAGNNNCTG	1 cut(s)	1131
PsuI	RGATCY	2 cut(s)	1273, 1564
RsaI	GTAC	4 cut(s)	70, 182, 425, 1043
RsaNI	GTAC	4 cut(s)	69, 181, 424, 1042
RseI	CAYNNNNRTG	3 cut(s)	720, 1253, 1777
SacI	GAGCTC	1 cut(s)	129
SaqAI	TTAA	5 cut(s)	779, 845, 1083, 1343, 1795
SatI	GCNGC	4 cut(s)	800, 928, 931, 1260
Sau96I	GGNCC	7 cut(s)	315, 572, 632, 674, 1381, 1467, 1760
SbfI	CCTGCAGG	1 cut(s)	148
ScaI	AGTACT	2 cut(s)	425, 1043
SchI	GAGTC	2 cut(s)	1000, 1493
ScrFI	CCNGG	9 cut(s)	12, 273, 449, 450, 577, 769, 1078, 1379, 1644
SdaI	CCTGCAGG	1 cut(s)	148
SduI	GDGCHC	2 cut(s)	129, 1124
SfaNI	GCATC	4 cut(s)	397, 520, 1104, 1271
SfcI	CTRYAG	3 cut(s)	144, 733, 797
SfuI	TTCGAA	1 cut(s)	1600
SinI	GGWCC	5 cut(s)	315, 572, 632, 674, 1467
SmaI	CCCGGG	1 cut(s)	450
SmiMI	CAYNNNNRTG	3 cut(s)	720, 1253, 1777
SmlI	CTYRAG	3 cut(s)	546, 783, 1100
SmoI	CTYRAG	3 cut(s)	546, 783, 1100
SnaBI	TACGTA	1 cut(s)	169
SphI	GCATGC	1 cut(s)	1707
Sse8387I	CCTGCAGG	1 cut(s)	148
SsiI	CCGC	2 cut(s)	336, 927
SspI	AATATT	1 cut(s)	907
SspMI	CTAG	5 cut(s)	464, 701, 1013, 1385, 1698
SstI	GAGCTC	1 cut(s)	129
StyD4I	CCNGG	9 cut(s)	10, 271, 447, 448, 575, 767, 1076, 1377, 1642
StyI	CCWWGG	1 cut(s)	635
TaaI	ACNGT	5 cut(s)	205, 310, 493, 866, 1133
TaiI	ACGT	1 cut(s)	171
TaqI	TCGA	4 cut(s)	163, 816, 1532, 1600
TatI	WGTACW	3 cut(s)	68, 423, 1041
TauI	GCSGC	1 cut(s)	930
TfiI	GAWTC	6 cut(s)	132, 242, 365, 645, 1447, 1534
Tru1I	TTAA	5 cut(s)	779, 845, 1083, 1343, 1795
Tru9I	TTAA	5 cut(s)	779, 845, 1083, 1343, 1795
TscAI	CASTG	5 cut(s)	354, 400, 661, 1124, 1231
TseFI	GTSAC	2 cut(s)	395, 866
TseI	GCWGC	3 cut(s)	799, 930, 1259
Tsp45I	GTSAC	2 cut(s)	395, 866
TspDTI	ATGAA	5 cut(s)	234, 488, 738, 1526, 1731
TspMI	CCCGGG	1 cut(s)	448
TspRI	CASTG	5 cut(s)	354, 400, 661, 1124, 1231
Van91I	CCANNNNNTGG	3 cut(s)	312, 641, 1364
VneI	GTGCAC	1 cut(s)	1120
VpaK11BI	GGWCC	5 cut(s)	315, 572, 632, 674, 1467
XagI	CCTNNNNNAGG	2 cut(s)	546, 669
XapI	RAATTY	5 cut(s)	276, 953, 1055, 1153, 1729
XbaI	TCTAGA	1 cut(s)	1697
XceI	RCATGY	3 cut(s)	1588, 1707, 1780
XmaI	CCCGGG	1 cut(s)	448
XmnI	GAANNNNTTC	2 cut(s)	594, 1132
XspI	CTAG	5 cut(s)	464, 701, 1013, 1385, 1698
ZrmI	AGTACT	2 cut(s)	425, 1043

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.