MD05G1232100.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000484
Physical Location & Seq
Forward (+)
36515102 .. 36521698
6597 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1232100.v1.1.491

Sequence Viewer

Length: 2457 bp
ATGCAAGATGGAATGGAACTCTGGTTGTGGGAGGAAGAAGCCAGTTCCTTACTATTTTTAGCAGTATTCTCCATAGCTGATGACGGCACAAGTACATTTCAGTCCATTAGTGACGGTGAGACTGTAGTTTCAACTGGCGGAACATTTGAGCTAGGATTTTACAGTCCCGATGCTTCTAATAGACGGTATGTGGGGATATGGTACAAGAAGATTTCTGTTAAAACCATTGTATGGGTTGCCAACAGAGACACACCCCTCACTGATTTGTTTGGCGTCCTGAAGATCACCAACCCCGGAATACTTGTCCTCAACCACAACATGAGCACAATTTGGTCCTCCAACACATCGAGAACTGCACAGAATCCAGTGGCACGTCTTTTGGACTCGGGTAATCTTGTTGTGGTAGATAGGAGTGATGATGACCCTGAGAACTTTCTGTGGCAAAGTTTTGATTACCCTGGCGATACATGCCTACCAGGTGCGAAGCTTGGTAGGAACACAGTTACAGGCTTCAATTGGCATCTTAGATCATGGAAAAGTCCTCAGGATCCTTCTCCAGGTAATTATACATATCAATTTGGTCCCAAAGGATATGCAGAAATGTTTCTGAGGGAAGGTTCTGTCATAAAATTTCGGAGTGGACCATGGAATGGAGTCCGGTTCAGTGGAATGCCGCAGTTAAGTCCAAACCCTATATACACATACGGTCTTGTTTTTGAGACTGATGAAATGTACTACAGTTACAAGCTTCGCAACAACTCAATCCTTTCGAACATGGTGTTAACTTCAGAGGGGCTTTCACGGCGCTATATATGGATTGATAGAACCAAAGATTGGTTTCTTTACCTAACAGCCCAGATTGATAACTGTAACAACTATGCATTCTGTGGTGTATATGGCACATGTAACATTGAAAAGTCCCCGGTATGTAGCTGTTTGAAAGGATTTACCCCAAAGTTTCCAAACGAATGGGATTTGGTGGATTGGTCAAATGGCTGTGTGAGAAAGACTTCTCTAAATTGCACCGGAGACGTGTTCCAAAAGTACTCGGGGTTGAAATTGCCTAGCACAGAACAATCCTGGTTTAACAGAAGTATGAACCTCAAGGAATGTGAGATGGTGTGCATGAAGAACTGCTCCTGCACGGCTTATACAAATTTGGATATCCGAGATGGAGGAAGCGGGTGTTTGCTGTGGTACGGCGATCTAATTGATATAAGATACTTGGCTGAAAACGGGCAAGATATTTATATAAGAATGGCTTCATCGGAACTAGACAATACAAAGATCGACGCTAAATACTCTGAATCCAATGTGAAGAAAATGAGAATCATAATAAGCACTACTGTGTTGTCTACCGGACTGCTGATCATGGGCCTGGCCCTGCTGTTTTATGTTTGGAAGAAGCAGCACCAGAAAGGTGGAAAACTGGGATGCGGCCAAAAGGAGGATCTTGAATTACCGTTATTTGATTTGATGACTGTAGTTTCTGCAACCAGTAACTTTTCAAGTGAAAACAAACTTGGTGAAGGAGGTTTCGGATCTGTCTTTAAGGGTACAATGAAAGATGGACAAGAAATCGCAGTGAAAAAGCTTTCAAAAAGTTCTCAACAAGGGCTCGACGAGTTCAAGAATGAAGTTACACATATTGCCAAACTTCAGCACAGGAATCTAGTGAAGCTTCTTGGATACTGCATTCAAGAAGACGAGATGATGCTGATCTACGAGTACATGCCTAACAAGAGCTTAGACTTCTTTATATTCGATCAAAGAAGAAGGATGTTATTAGACTGGCCGAAGCGCTTTGAAATTATTAATGGGATAGCTCGAGGGATCCTCTATCTTCATCAAGATTCTAGACTGAGAGTTATCCATAGAGATCTCAAAGCAAGTAACATTTTGTTAGGCAGTGAATTTAATCCGAAAATCTCAGACTTTGGCCTGGCTAGAAGCTTTGGAGGAAATGAAACGAAAACAAAAACGAAGAAAGTGGTCGGAACATACGGTTACATGTCCCCAGAATATGCAATTGATGGTTTCTACTCTATAAAGTCCGACGTCTATAGCTTTGGTGTTATGGTGCTAGAGATAGTGAGTGGGAGCAGAAATAGAGGATTCTCTCATCCAGACCACAAACTCAACCTTCTTGGACATGCGTGGATGCTACACACAGAAGGCAGGCCTCTCGAATTGCTTGATACATCGGTAGAGGACTCCATCACTTTGCATGAAGTTGTACGAACAATTCATGTGGGTCTTTTGTGTGTGCAGAGGAATCCAGAAGATAGGCCAAGCATGTCGGCTGCAGTTCTAATGTTGGGTGGTGAAGGTGCATTGCCCCCACCTCTAAAACCTGGTTTCTACAGTGAAAGGGATTTGACTGAACTTGATGCCGGTCATTCTTCTAAAGCATGCTCAGCTAATGATGTCACTATTTCACTACTTGAGGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

819

Amino Acids

92.17

Weight (kDa)

6.22

Isoelectric Point (pI)

43.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 75 - 179 2.5e-36 D-mannose binding lectin
S_locus_glycop PF00954 211 - 320 3e-31 S-locus glycoprotein domain
PAN_2 PF08276 341 - 406 4.1e-20 PAN-like domain
Pkinase PF00069 503 - 769 3.4e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 505 - 768 1e-48 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 777 - 818 6.5e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2061
AccB7I CCANNNNNTGG 3 cut(s) 231, 650, 834
AccI GTMKAC 1 cut(s) 1355
AciI CCGC 4 cut(s) 138, 674, 1182, 1437
AclWI GGATC 6 cut(s) 542, 555, 1458, 1549, 1828, 1841
AcoI YGGCCR 2 cut(s) 1438, 1793
AcsI RAATTY 3 cut(s) 629, 1156, 1913
AcuI CTGAAG 3 cut(s) 299, 771, 1643
AcyI GRCGYC 2 cut(s) 273, 2058
AfaI GTAC 8 cut(s) 94, 203, 734, 1046, 1199, 1558, 1730, 2238
AfeI AGCGCT 1 cut(s) 1802
AfiI CCNNNNNNNGG 5 cut(s) 231, 557, 650, 834, 1447
AflIII ACRYGT 3 cut(s) 902, 1032, 2010
AjiI CACGTC 2 cut(s) 374, 1033
AjnI CCWGG 7 cut(s) 457, 475, 556, 1079, 1377, 1941, 2353
AjuI GAANNNNNNNTTGG 4 cut(s) 817, 849, 1506, 1538
AleI CACNNNNGTG 1 cut(s) 1346
Alw21I GWGCWC 1 cut(s) 326
Alw26I GTCTC 4 cut(s) 113, 240, 713, 1023
AlwI GGATC 6 cut(s) 542, 555, 1458, 1549, 1828, 1841
Ama87I CYCGRG 3 cut(s) 385, 1048, 1827
Aor51HI AGCGCT 1 cut(s) 1802
AoxI GGCC 7 cut(s) 1375, 1380, 1438, 1793, 1939, 2180, 2288
ApeKI GCWGC 2 cut(s) 1408, 2303
ApoI RAATTY 3 cut(s) 629, 1156, 1913
AseI ATTAAT 1 cut(s) 1815
Asp700I GAANNNNTTC 3 cut(s) 603, 1009, 1261
AspLEI GCGC 2 cut(s) 807, 1803
AspS9I GGNCC 5 cut(s) 333, 581, 641, 1375, 1381
AsuC2I CCSGG 2 cut(s) 294, 923
AsuHPI GGTGA 4 cut(s) 128, 277, 1538, 2336
AsuII TTCGAA 1 cut(s) 770
AvaI CYCGRG 3 cut(s) 385, 1048, 1827
AvaII GGWCC 3 cut(s) 333, 581, 641
AxyI CCTNAGG 1 cut(s) 543
BaeI ACNNNNGTAYC 2 cut(s) 2190, 2223
BamHI GGATCC 2 cut(s) 547, 1833
BanII GRGCYC 1 cut(s) 1620
BbsI GAAGAC 1 cut(s) 1710
Bbv12I GWGCWC 1 cut(s) 326
BbvI GCAGC 2 cut(s) 1420, 2290
BccI CCATC 6 cut(s) 2, 1111, 1166, 1562, 2027, 2225
BceAI ACGGC 4 cut(s) 100, 818, 1161, 1216
BcgI CGANNNNNNTGC 2 cut(s) 2167, 2201
BciT130I CCWGG 7 cut(s) 459, 477, 558, 1081, 1379, 1943, 2355
BciVI GTATCC 1 cut(s) 1682
BclI TGATCA 1 cut(s) 1368
BcnI CCSGG 2 cut(s) 294, 923
BcoDI GTCTC 4 cut(s) 113, 240, 713, 1023
BfaI CTAG 7 cut(s) 152, 1065, 1274, 1673, 1857, 1947, 2084
BfmI CTRYAG 6 cut(s) 123, 736, 1482, 2062, 2304, 2362
BfoI RGCGCY 2 cut(s) 808, 1804
BfuI GTATCC 1 cut(s) 1682
BglII AGATCT 1 cut(s) 1879
BisI GCNGC 4 cut(s) 674, 1409, 1438, 2304
BlpI GCTNAGC 1 cut(s) 2416
BlsI GCNGC 4 cut(s) 675, 1410, 1439, 2305
BmcAI AGTACT 1 cut(s) 1046
Bme1390I CCNGG 9 cut(s) 294, 459, 477, 558, 923, 1081, 1379, 1943, 2355
Bme18I GGWCC 3 cut(s) 333, 581, 641
BmeT110I CYCGRG 3 cut(s) 385, 1048, 1827
BmgBI CACGTC 2 cut(s) 374, 1033
BmgT120I GGNCC 5 cut(s) 333, 581, 641, 1375, 1381
BmiI GGNNCC 3 cut(s) 549, 583, 1835
BmrFI CCNGG 9 cut(s) 294, 459, 477, 558, 923, 1081, 1379, 1943, 2355
BmrI ACTGGG 1 cut(s) 1439
BmsI GCATC 6 cut(s) 160, 529, 1424, 1704, 2151, 2380
BmuI ACTGGG 1 cut(s) 1439
BpiI GAAGAC 1 cut(s) 1710
BplI GAGNNNNNCTC 2 cut(s) 1821, 1853
BpmI CTGGAG 1 cut(s) 540
Bpu1102I GCTNAGC 1 cut(s) 2416
Bpu14I TTCGAA 1 cut(s) 770
BpuEI CTTGAG 1 cut(s) 1088
BpuMI CCSGG 2 cut(s) 294, 923
BsaBI GATNNNNATC 1 cut(s) 1718
BsaHI GRCGYC 2 cut(s) 273, 2058
BsaJI CCNNGG 4 cut(s) 292, 457, 644, 921
BsaWI WCCGGW 3 cut(s) 657, 1025, 1358
Bsc4I CCNNNNNNNGG 5 cut(s) 231, 557, 650, 834, 1447
Bse118I RCCGGY 1 cut(s) 2393
Bse1I ACTGG 6 cut(s) 42, 139, 365, 1434, 1497, 1796
Bse21I CCTNAGG 1 cut(s) 543
Bse3DI GCAATG 1 cut(s) 2333
Bse8I GATNNNNATC 1 cut(s) 1718
BseBI CCWGG 7 cut(s) 459, 477, 558, 1081, 1379, 1943, 2355
BseDI CCNNGG 4 cut(s) 292, 457, 644, 921
BseGI GGATG 4 cut(s) 1439, 1785, 2122, 2166
BseJI GATNNNNATC 1 cut(s) 1718
BseLI CCNNNNNNNGG 5 cut(s) 231, 557, 650, 834, 1447
BseMI GCAATG 1 cut(s) 2333
BseMII CTCAG 6 cut(s) 417, 557, 599, 1853, 1944, 2430
BseNI ACTGG 6 cut(s) 42, 139, 365, 1434, 1497, 1796
BseXI GCAGC 2 cut(s) 1420, 2290
BsgI GTGCAG 3 cut(s) 339, 1126, 2288
BshFI GGCC 7 cut(s) 1377, 1382, 1440, 1795, 1941, 2182, 2290
BsiHKAI GWGCWC 1 cut(s) 326
BsiHKCI CYCGRG 3 cut(s) 385, 1048, 1827
BsiSI CCGG 6 cut(s) 294, 658, 923, 1026, 1359, 2394
BslFI GGGAC 4 cut(s) 150, 567, 904, 1999
BslI CCNNNNNNNGG 5 cut(s) 231, 557, 650, 834, 1447
BsmAI GTCTC 4 cut(s) 113, 240, 713, 1023
BsmBI CGTCTC 1 cut(s) 1023
BsmFI GGGAC 4 cut(s) 150, 567, 904, 1999
BsmI GAATGC 3 cut(s) 675, 881, 1695
BsnI GGCC 7 cut(s) 1377, 1382, 1440, 1795, 1941, 2182, 2290
BsoBI CYCGRG 3 cut(s) 385, 1048, 1827
Bsp119I TTCGAA 1 cut(s) 770
Bsp1286I GDGCHC 2 cut(s) 326, 1620
Bsp1720I GCTNAGC 1 cut(s) 2416
Bsp19I CCATGG 1 cut(s) 644
BspACI CCGC 4 cut(s) 138, 674, 1182, 1437
BspANI GGCC 7 cut(s) 1377, 1382, 1440, 1795, 1941, 2182, 2290
BspCNI CTCAG 6 cut(s) 418, 556, 600, 1854, 1943, 2429
BspLI GGNNCC 3 cut(s) 549, 583, 1835
BspMAI CTGCAG 1 cut(s) 2308
BspPI GGATC 6 cut(s) 542, 555, 1458, 1549, 1828, 1841
BspT104I TTCGAA 1 cut(s) 770
BsrDI GCAATG 1 cut(s) 2333
BsrFI RCCGGY 1 cut(s) 2393
BsrI ACTGG 6 cut(s) 42, 139, 365, 1434, 1497, 1796
BssAI RCCGGY 1 cut(s) 2393
BssECI CCNNGG 4 cut(s) 292, 457, 644, 921
BssNI GRCGYC 2 cut(s) 273, 2058
BssT1I CCWWGG 1 cut(s) 644
Bst2UI CCWGG 7 cut(s) 459, 477, 558, 1081, 1379, 1943, 2355
BstACI GRCGYC 2 cut(s) 273, 2058
BstBI TTCGAA 1 cut(s) 770
BstC8I GCNNGC 2 cut(s) 2180, 2413
BstDEI CTNAG 8 cut(s) 426, 524, 543, 608, 1747, 1862, 1930, 2416
BstDSI CCRYGG 1 cut(s) 644
BstENI CCTNNNNNAGG 1 cut(s) 555
BstF5I GGATG 4 cut(s) 1439, 1785, 2122, 2166
BstH2I RGCGCY 2 cut(s) 808, 1804
BstHHI GCGC 2 cut(s) 807, 1803
BstMAI GTCTC 4 cut(s) 113, 240, 713, 1023
BstMWI GCNNNNNNNGC 3 cut(s) 468, 802, 2417
BstNI CCWGG 7 cut(s) 459, 477, 558, 1081, 1379, 1943, 2355
BstNSI RCATGY 7 cut(s) 471, 906, 1735, 2014, 2156, 2299, 2415
BstSCI CCNGG 9 cut(s) 292, 457, 475, 556, 921, 1079, 1377, 1941, 2353
BstSFI CTRYAG 6 cut(s) 123, 736, 1482, 2062, 2304, 2362
BstV1I GCAGC 2 cut(s) 1420, 2290
BstV2I GAAGAC 1 cut(s) 1710
BstX2I RGATCY 5 cut(s) 547, 1450, 1541, 1833, 1879
BstXI CCANNNNNNTGG 2 cut(s) 969, 1421
BstYI RGATCY 5 cut(s) 547, 1450, 1541, 1833, 1879
Bsu36I CCTNAGG 1 cut(s) 543
BsuI GTATCC 1 cut(s) 1682
BsuRI GGCC 7 cut(s) 1377, 1382, 1440, 1795, 1941, 2182, 2290
BtgI CCRYGG 1 cut(s) 644
BtrI CACGTC 2 cut(s) 374, 1033
BtsCI GGATG 4 cut(s) 1439, 1785, 2122, 2166
BtsI GCAGTG 2 cut(s) 1590, 1915
BtsIMutI CAGTG 6 cut(s) 258, 372, 670, 1590, 1915, 2371
Cac8I GCNNGC 2 cut(s) 2180, 2413
CfoI GCGC 2 cut(s) 807, 1803
Cfr10I RCCGGY 1 cut(s) 2393
Cfr13I GGNCC 5 cut(s) 333, 581, 641, 1375, 1381
CseI GACGC 2 cut(s) 262, 1301
CsiI ACCWGGT 2 cut(s) 475, 2353
Csp6I GTAC 8 cut(s) 93, 202, 733, 1045, 1198, 1557, 1729, 2237
CspCI CAANNNNNGTGG 2 cut(s) 2232, 2267
CviQI GTAC 8 cut(s) 93, 202, 733, 1045, 1198, 1557, 1729, 2237
DdeI CTNAG 8 cut(s) 426, 524, 543, 608, 1747, 1862, 1930, 2416
EaeI YGGCCR 2 cut(s) 1438, 1793
EciI GGCGGA 1 cut(s) 153
Eco130I CCWWGG 1 cut(s) 644
Eco147I AGGCCT 1 cut(s) 2182
Eco24I GRGCYC 1 cut(s) 1620
Eco32I GATATC 1 cut(s) 1165
Eco47I GGWCC 3 cut(s) 333, 581, 641
Eco47III AGCGCT 1 cut(s) 1802
Eco57I CTGAAG 3 cut(s) 299, 771, 1643
Eco81I CCTNAGG 1 cut(s) 543
Eco88I CYCGRG 3 cut(s) 385, 1048, 1827
EcoNI CCTNNNNNAGG 1 cut(s) 555
EcoRII CCWGG 7 cut(s) 457, 475, 556, 1079, 1377, 1941, 2353
EcoRV GATATC 1 cut(s) 1165
EcoT14I CCWWGG 1 cut(s) 644
EcoT22I ATGCAT 1 cut(s) 883
EcoT38I GRGCYC 1 cut(s) 1620
ErhI CCWWGG 1 cut(s) 644
Esp3I CGTCTC 1 cut(s) 1023
FalI AAGNNNNNCTT 2 cut(s) 1246, 1278
FaqI GGGAC 4 cut(s) 150, 567, 904, 1999
FauI CCCGC 1 cut(s) 1175
FbaI TGATCA 1 cut(s) 1368
FblI GTMKAC 1 cut(s) 1355
Fnu4HI GCNGC 4 cut(s) 674, 1409, 1438, 2304
FokI GGATG 4 cut(s) 1446, 1792, 2109, 2173
FriOI GRGCYC 1 cut(s) 1620
Fsp4HI GCNGC 4 cut(s) 674, 1409, 1438, 2304
FspBI CTAG 7 cut(s) 152, 1065, 1274, 1673, 1857, 1947, 2084
GlaI GCGC 2 cut(s) 806, 1802
GluI GCNGC 4 cut(s) 674, 1409, 1438, 2304
GsuI CTGGAG 1 cut(s) 540
HaeII RGCGCY 2 cut(s) 808, 1804
HaeIII GGCC 7 cut(s) 1377, 1382, 1440, 1795, 1941, 2182, 2290
HapII CCGG 6 cut(s) 294, 658, 923, 1026, 1359, 2394
HgaI GACGC 2 cut(s) 262, 1301
HhaI GCGC 2 cut(s) 807, 1803
Hin1I GRCGYC 2 cut(s) 273, 2058
Hin6I GCGC 2 cut(s) 805, 1801
HinP1I GCGC 2 cut(s) 805, 1801
HincII GTYRAC 1 cut(s) 783
HindII GTYRAC 1 cut(s) 783
HindIII AAGCTT 5 cut(s) 485, 746, 1592, 1679, 1951
HpaI GTTAAC 1 cut(s) 783
HpaII CCGG 6 cut(s) 294, 658, 923, 1026, 1359, 2394
HphI GGTGA 4 cut(s) 128, 277, 1538, 2336
Hpy166II GTNNAC 3 cut(s) 641, 783, 1356
Hpy8I GTNNAC 3 cut(s) 641, 783, 1356
Hpy99I CGWCG 3 cut(s) 1295, 1625, 2060
HpyAV CCTTC 7 cut(s) 561, 608, 1523, 1770, 2153, 2168, 2321
HpyCH4IV ACGT 3 cut(s) 373, 1032, 2058
HpyF10VI GCNNNNNNNGC 3 cut(s) 468, 802, 2417
HpyF3I CTNAG 8 cut(s) 426, 524, 543, 608, 1747, 1862, 1930, 2416
HpySE526I ACGT 3 cut(s) 373, 1032, 2058
Hsp92I GRCGYC 2 cut(s) 273, 2058
HspAI GCGC 2 cut(s) 805, 1801
Ksp22I TGATCA 1 cut(s) 1368
KspAI GTTAAC 1 cut(s) 783
LmnI GCTCC 2 cut(s) 1142, 2100
Lsp1109I GCAGC 2 cut(s) 1420, 2290
LweI GCATC 6 cut(s) 160, 529, 1424, 1704, 2151, 2380
MabI ACCWGGT 2 cut(s) 475, 2353
MaeI CTAG 7 cut(s) 152, 1065, 1274, 1673, 1857, 1947, 2084
MaeII ACGT 3 cut(s) 373, 1032, 2058
MfeI CAATTG 2 cut(s) 514, 2028
MflI RGATCY 5 cut(s) 547, 1450, 1541, 1833, 1879
MhlI GDGCHC 2 cut(s) 326, 1620
MlyI GAGTC 3 cut(s) 377, 663, 2207
MmeI TCCRAC 3 cut(s) 363, 1975, 2079
Mph1103I ATGCAT 1 cut(s) 883
MroXI GAANNNNTTC 3 cut(s) 603, 1009, 1261
MseI TTAA 7 cut(s) 219, 680, 782, 1086, 1551, 1815, 1917
MslI CAYNNNNRTG 1 cut(s) 1346
MspI CCGG 6 cut(s) 294, 658, 923, 1026, 1359, 2394
MspR9I CCNGG 9 cut(s) 294, 459, 477, 558, 923, 1081, 1379, 1943, 2355
MunI CAATTG 2 cut(s) 514, 2028
Mva1269I GAATGC 3 cut(s) 675, 881, 1695
MvaI CCWGG 7 cut(s) 459, 477, 558, 1081, 1379, 1943, 2355
MwoI GCNNNNNNNGC 3 cut(s) 468, 802, 2417
NciI CCSGG 2 cut(s) 294, 923
NcoI CCATGG 1 cut(s) 644
NlaIV GGNNCC 3 cut(s) 549, 583, 1835
NmuCI GTSAC 2 cut(s) 110, 2428
NsiI ATGCAT 1 cut(s) 883
NspI RCATGY 7 cut(s) 471, 906, 1735, 2014, 2156, 2299, 2415
NspV TTCGAA 1 cut(s) 770
OliI CACNNNNGTG 1 cut(s) 1346
PaeI GCATGC 1 cut(s) 2415
PaeR7I CTCGAG 1 cut(s) 1827
PceI AGGCCT 1 cut(s) 2182
PciI ACATGT 2 cut(s) 902, 2010
PctI GAATGC 3 cut(s) 675, 881, 1695
PdmI GAANNNNTTC 3 cut(s) 603, 1009, 1261
PfeI GAWTC 7 cut(s) 361, 1307, 1329, 1669, 1853, 2115, 2275
PflMI CCANNNNNTGG 3 cut(s) 231, 650, 834
PkrI GCNGC 4 cut(s) 675, 1410, 1439, 2305
PleI GAGTC 3 cut(s) 377, 662, 2207
PpsI GAGTC 3 cut(s) 377, 662, 2207
PscI ACATGT 2 cut(s) 902, 2010
PshBI ATTAAT 1 cut(s) 1815
Psp6I CCWGG 7 cut(s) 457, 475, 556, 1079, 1377, 1941, 2353
PspGI CCWGG 7 cut(s) 457, 475, 556, 1079, 1377, 1941, 2353
PspN4I GGNNCC 3 cut(s) 549, 583, 1835
PspPI GGNCC 5 cut(s) 333, 581, 641, 1375, 1381
PspXI VCTCGAGB 1 cut(s) 1827
PstI CTGCAG 1 cut(s) 2308
PsuI RGATCY 5 cut(s) 547, 1450, 1541, 1833, 1879
RsaI GTAC 8 cut(s) 94, 203, 734, 1046, 1199, 1558, 1730, 2238
RsaNI GTAC 8 cut(s) 93, 202, 733, 1045, 1198, 1557, 1729, 2237
RseI CAYNNNNRTG 1 cut(s) 1346
SaqAI TTAA 7 cut(s) 219, 680, 782, 1086, 1551, 1815, 1917
SatI GCNGC 4 cut(s) 674, 1409, 1438, 2304
Sau96I GGNCC 5 cut(s) 333, 581, 641, 1375, 1381
ScaI AGTACT 1 cut(s) 1046
SchI GAGTC 3 cut(s) 377, 663, 2207
ScrFI CCNGG 9 cut(s) 294, 459, 477, 558, 923, 1081, 1379, 1943, 2355
SduI GDGCHC 2 cut(s) 326, 1620
SexAI ACCWGGT 2 cut(s) 475, 2353
SfaNI GCATC 6 cut(s) 160, 529, 1424, 1704, 2151, 2380
SfcI CTRYAG 6 cut(s) 123, 736, 1482, 2062, 2304, 2362
Sfr274I CTCGAG 1 cut(s) 1827
SfuI TTCGAA 1 cut(s) 770
SinI GGWCC 3 cut(s) 333, 581, 641
SlaI CTCGAG 1 cut(s) 1827
SmiMI CAYNNNNRTG 1 cut(s) 1346
SmlI CTYRAG 3 cut(s) 1103, 1827, 2444
SmoI CTYRAG 3 cut(s) 1103, 1827, 2444
SphI GCATGC 1 cut(s) 2415
SseBI AGGCCT 1 cut(s) 2182
SsiI CCGC 4 cut(s) 138, 674, 1182, 1437
SspMI CTAG 7 cut(s) 152, 1065, 1274, 1673, 1857, 1947, 2084
StuI AGGCCT 1 cut(s) 2182
StyD4I CCNGG 9 cut(s) 292, 457, 475, 556, 921, 1079, 1377, 1941, 2353
StyI CCWWGG 1 cut(s) 644
TaiI ACGT 3 cut(s) 376, 1035, 2061
TaqI TCGA 8 cut(s) 347, 770, 1290, 1620, 1764, 1828, 2187, 2452
TatI WGTACW 4 cut(s) 92, 732, 1044, 1728
TauI GCSGC 2 cut(s) 676, 1440
TfiI GAWTC 7 cut(s) 361, 1307, 1329, 1669, 1853, 2115, 2275
Tru1I TTAA 7 cut(s) 219, 680, 782, 1086, 1551, 1815, 1917
Tru9I TTAA 7 cut(s) 219, 680, 782, 1086, 1551, 1815, 1917
TscAI CASTG 6 cut(s) 265, 372, 670, 1590, 1915, 2371
TseFI GTSAC 2 cut(s) 110, 2428
TseI GCWGC 2 cut(s) 1408, 2303
Tsp45I GTSAC 2 cut(s) 110, 2428
TspRI CASTG 6 cut(s) 265, 372, 670, 1590, 1915, 2371
Van91I CCANNNNNTGG 3 cut(s) 231, 650, 834
VpaK11BI GGWCC 3 cut(s) 333, 581, 641
VspI ATTAAT 1 cut(s) 1815
XagI CCTNNNNNAGG 1 cut(s) 555
XapI RAATTY 3 cut(s) 629, 1156, 1913
XbaI TCTAGA 1 cut(s) 1856
XceI RCATGY 7 cut(s) 471, 906, 1735, 2014, 2156, 2299, 2415
XhoI CTCGAG 1 cut(s) 1827
XmiI GTMKAC 1 cut(s) 1355
XmnI GAANNNNTTC 3 cut(s) 603, 1009, 1261
XspI CTAG 7 cut(s) 152, 1065, 1274, 1673, 1857, 1947, 2084
ZraI GACGTC 1 cut(s) 2059
ZrmI AGTACT 1 cut(s) 1046
Zsp2I ATGCAT 1 cut(s) 883
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.