pycom05g20380

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000484
Physical Location & Seq
Reverse (-)
23144873 .. 23146303
1431 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g20380.1

Sequence Viewer

Length: 981 bp
ATGACTATAGTTTCTGCAACCAGTAACTTCTCAATTGAAAACAAACTTGGTGAAGGTGGTTTCGGATCTGTCTTCAAGGGTACGATGGAAGATGGACAAGAAATCGCAGTGAAAAGGCTTTCAAAAAGTTCTAGACAAGGACTTAACGAGTTCAAGAATGAAGTTACACATATTGCCAAACTTCAGCACAGGAATCTAGTGAAGCTTCTTGGATGCTGCATTCAAGAAGACGAGATGATACTGATCTACGAGTTCATGCCTAACAAGAGCTTAGACTTCTTTATTTTCGATCAAAGAAGAAGGATGTTATTAGACTGGCCGAAGCGCTTTGAAATTATTAATGGGATAGCTCGAGGGCTCCTCTATCTTCATCAAGATTCTAGATTGAGAGTTATTCATAGAGATCTCAAAGCAAGTAACATTTTATTAGGAAGTGAATTCAACCCGAAAATCTCAGACTTTGGCTTGGCTAGAAGCTTTGGAGGAAATGAAACGAAAGCAGAAACGAAGAAAGTGGTCGGAACATACGGTTACATGTCCCCAGAATATGCAATTGATGGTTTCTACTCTATAAAGTCCGACGTCTATAGCTTTGGTGTTCTGGTGCTAGAGATGGTGAGTGGGAGCAGAAATAGAGGATTCTCTCATCCAGACCACAAACTTAACCTTCTTGGACATGCATGGATGCTACACACAGAAGGCAGGCATCTTGAACTGCTTGATGCATCGATAGAGGACTCCATCACTTTGCATGAAGTTGTTAGAACGATTCATGTGGGTCTTTTGTGCGTGCAGCGGAATCCAGAAGACAGGCCGAGCATGTCAGCTGCAGTTCTAATGTTGGGTAGTGAAGGTGCGTTGCCCCCACCTCTAAAACCTGGTTTTTACAGTGAAAGGGATTTGACTGAACTCGAAGTCGGTCATTCTTCTAAAGCATGCTCAGCTAATGAAGTCACTATTTCACTAGTCGAGGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

327

Amino Acids

36.55

Weight (kDa)

6.47

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 11 - 277 1e-48 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 11 - 277 6.8e-47 Protein kinase domain
DUF3403 PF11883 284 - 326 1.6e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 585
AciI CCGC 1 cut(s) 796
AclWI GGATC 1 cut(s) 73
AcoI YGGCCR 1 cut(s) 317
AcsI RAATTY 1 cut(s) 437
AcuI CTGAAG 1 cut(s) 167
AcyI GRCGYC 1 cut(s) 582
AfaI GTAC 1 cut(s) 82
AfeI AGCGCT 1 cut(s) 326
AflIII ACRYGT 1 cut(s) 534
AgsI TTSAA 8 cut(s) 38, 76, 123, 154, 224, 332, 442, 713
AhlI ACTAGT 1 cut(s) 964
AjnI CCWGG 1 cut(s) 877
AjuI GAANNNNNNNTTGG 2 cut(s) 30, 62
AluBI AGCT 7 cut(s) 205, 270, 350, 477, 591, 827, 944
AluI AGCT 7 cut(s) 205, 270, 350, 477, 591, 827, 944
AlwI GGATC 1 cut(s) 73
Ama87I CYCGRG 1 cut(s) 351
Aor51HI AGCGCT 1 cut(s) 326
AoxI GGCC 2 cut(s) 317, 812
ApeKI GCWGC 3 cut(s) 216, 793, 827
ApoI RAATTY 1 cut(s) 437
AseI ATTAAT 1 cut(s) 339
AspLEI GCGC 1 cut(s) 327
AsuHPI GGTGA 2 cut(s) 62, 628
AvaI CYCGRG 1 cut(s) 351
BanII GRGCYC 1 cut(s) 360
BbsI GAAGAC 3 cut(s) 64, 234, 813
BbvI GCAGC 3 cut(s) 203, 805, 814
BccI CCATC 5 cut(s) 79, 86, 551, 607, 749
BciT130I CCWGG 1 cut(s) 879
BcuI ACTAGT 1 cut(s) 964
BfaI CTAG 6 cut(s) 132, 197, 381, 471, 608, 965
BfmI CTRYAG 3 cut(s) 6, 586, 828
BfoI RGCGCY 1 cut(s) 328
BglII AGATCT 1 cut(s) 403
BisI GCNGC 3 cut(s) 217, 794, 828
BlpI GCTNAGC 1 cut(s) 940
BlsI GCNGC 3 cut(s) 218, 795, 829
Bme1390I CCNGG 1 cut(s) 879
BmeT110I CYCGRG 1 cut(s) 351
BmiI GGNNCC 1 cut(s) 359
BmrFI CCNGG 1 cut(s) 879
BmsI GCATC 5 cut(s) 203, 675, 712, 715, 734
BpiI GAAGAC 3 cut(s) 64, 234, 813
BplI GAGNNNNNCTC 2 cut(s) 345, 377
Bpu1102I GCTNAGC 1 cut(s) 940
Bsa29I ATCGAT 1 cut(s) 728
BsaBI GATNNNNATC 1 cut(s) 242
BsaHI GRCGYC 1 cut(s) 582
Bse1I ACTGG 2 cut(s) 21, 320
Bse8I GATNNNNATC 1 cut(s) 242
BseBI CCWGG 1 cut(s) 879
BseCI ATCGAT 1 cut(s) 728
BseGI GGATG 4 cut(s) 218, 309, 646, 690
BseJI GATNNNNATC 1 cut(s) 242
BseMII CTCAG 2 cut(s) 468, 954
BseNI ACTGG 2 cut(s) 21, 320
BseRI GAGGAG 1 cut(s) 350
BseXI GCAGC 3 cut(s) 203, 805, 814
BsgI GTGCAG 1 cut(s) 812
BshFI GGCC 2 cut(s) 319, 814
BshVI ATCGAT 1 cut(s) 728
BsiHKCI CYCGRG 1 cut(s) 351
BslFI GGGAC 1 cut(s) 523
BsmFI GGGAC 1 cut(s) 523
BsmI GAATGC 1 cut(s) 219
BsnI GGCC 2 cut(s) 319, 814
BsoBI CYCGRG 1 cut(s) 351
Bsp1286I GDGCHC 1 cut(s) 360
Bsp143I GATC 4 cut(s) 65, 243, 289, 403
Bsp1720I GCTNAGC 1 cut(s) 940
BspACI CCGC 1 cut(s) 796
BspANI GGCC 2 cut(s) 319, 814
BspCNI CTCAG 2 cut(s) 467, 953
BspDI ATCGAT 1 cut(s) 728
BspLI GGNNCC 1 cut(s) 359
BspMAI CTGCAG 1 cut(s) 832
BspPI GGATC 1 cut(s) 73
BsrI ACTGG 2 cut(s) 21, 320
BssMI GATC 4 cut(s) 65, 243, 289, 403
BssNI GRCGYC 1 cut(s) 582
Bst2UI CCWGG 1 cut(s) 879
Bst4CI ACNGT 2 cut(s) 530, 890
BstACI GRCGYC 1 cut(s) 582
BstC8I GCNNGC 3 cut(s) 704, 791, 937
BstDEI CTNAG 3 cut(s) 271, 454, 940
BstF5I GGATG 4 cut(s) 218, 309, 646, 690
BstH2I RGCGCY 1 cut(s) 328
BstHHI GCGC 1 cut(s) 327
BstKTI GATC 4 cut(s) 68, 246, 292, 406
BstMBI GATC 4 cut(s) 65, 243, 289, 403
BstMWI GCNNNNNNNGC 1 cut(s) 941
BstNI CCWGG 1 cut(s) 879
BstNSI RCATGY 4 cut(s) 538, 680, 823, 939
BstSCI CCNGG 1 cut(s) 877
BstSFI CTRYAG 3 cut(s) 6, 586, 828
BstV1I GCAGC 3 cut(s) 203, 805, 814
BstV2I GAAGAC 3 cut(s) 64, 234, 813
BstX2I RGATCY 2 cut(s) 65, 403
BstYI RGATCY 2 cut(s) 65, 403
Bsu15I ATCGAT 1 cut(s) 728
BsuRI GGCC 2 cut(s) 319, 814
BsuTUI ATCGAT 1 cut(s) 728
BtsCI GGATG 4 cut(s) 218, 309, 646, 690
BtsI GCAGTG 1 cut(s) 114
BtsIMutI CAGTG 2 cut(s) 114, 895
Cac8I GCNNGC 3 cut(s) 704, 791, 937
CfoI GCGC 1 cut(s) 327
ClaI ATCGAT 1 cut(s) 728
CsiI ACCWGGT 1 cut(s) 877
Csp6I GTAC 1 cut(s) 81
CviAII CATG 8 cut(s) 256, 535, 677, 681, 752, 773, 820, 936
CviQI GTAC 1 cut(s) 81
DdeI CTNAG 3 cut(s) 271, 454, 940
DpnI GATC 4 cut(s) 67, 245, 291, 405
DpnII GATC 4 cut(s) 65, 243, 289, 403
EaeI YGGCCR 1 cut(s) 317
Eco24I GRGCYC 1 cut(s) 360
Eco47III AGCGCT 1 cut(s) 326
Eco57I CTGAAG 1 cut(s) 167
Eco88I CYCGRG 1 cut(s) 351
EcoRI GAATTC 1 cut(s) 437
EcoRII CCWGG 1 cut(s) 877
EcoT22I ATGCAT 2 cut(s) 682, 727
EcoT38I GRGCYC 1 cut(s) 360
FaeI CATG 8 cut(s) 259, 538, 680, 684, 755, 776, 823, 939
FaqI GGGAC 1 cut(s) 523
FatI CATG 8 cut(s) 255, 534, 676, 680, 751, 772, 819, 935
Fnu4HI GCNGC 3 cut(s) 217, 794, 828
FokI GGATG 4 cut(s) 225, 316, 633, 697
FriOI GRGCYC 1 cut(s) 360
Fsp4HI GCNGC 3 cut(s) 217, 794, 828
FspBI CTAG 6 cut(s) 132, 197, 381, 471, 608, 965
GlaI GCGC 1 cut(s) 326
GluI GCNGC 3 cut(s) 217, 794, 828
HaeII RGCGCY 1 cut(s) 328
HaeIII GGCC 2 cut(s) 319, 814
HhaI GCGC 1 cut(s) 327
Hin1I GRCGYC 1 cut(s) 582
Hin1II CATG 8 cut(s) 259, 538, 680, 684, 755, 776, 823, 939
Hin6I GCGC 1 cut(s) 325
HinP1I GCGC 1 cut(s) 325
HindIII AAGCTT 2 cut(s) 203, 475
HinfI GANTC 6 cut(s) 193, 377, 639, 737, 769, 799
HphI GGTGA 2 cut(s) 62, 628
Hpy188I TCNGA 4 cut(s) 65, 457, 521, 580
Hpy188III TCNNGA 8 cut(s) 132, 154, 224, 374, 381, 650, 710, 803
Hpy99I CGWCG 1 cut(s) 584
HpyAV CCTTC 5 cut(s) 47, 294, 677, 692, 845
HpyCH4III ACNGT 2 cut(s) 530, 890
HpyCH4IV ACGT 1 cut(s) 582
HpyCH4V TGCA 8 cut(s) 17, 219, 551, 680, 725, 751, 793, 830
HpyF10VI GCNNNNNNNGC 1 cut(s) 941
HpyF3I CTNAG 3 cut(s) 271, 454, 940
HpySE526I ACGT 1 cut(s) 582
Hsp92I GRCGYC 1 cut(s) 582
Hsp92II CATG 8 cut(s) 259, 538, 680, 684, 755, 776, 823, 939
HspAI GCGC 1 cut(s) 325
Kzo9I GATC 4 cut(s) 65, 243, 289, 403
LmnI GCTCC 2 cut(s) 363, 624
Lsp1109I GCAGC 3 cut(s) 203, 805, 814
LweI GCATC 5 cut(s) 203, 675, 712, 715, 734
MabI ACCWGGT 1 cut(s) 877
MaeI CTAG 6 cut(s) 132, 197, 381, 471, 608, 965
MaeII ACGT 1 cut(s) 582
MaeIII GTNAC 5 cut(s) 23, 163, 416, 530, 952
MalI GATC 4 cut(s) 67, 245, 291, 405
MboI GATC 4 cut(s) 65, 243, 289, 403
MboII GAAGA 8 cut(s) 64, 101, 239, 309, 359, 520, 818, 918
MfeI CAATTG 2 cut(s) 33, 552
MflI RGATCY 2 cut(s) 65, 403
MhlI GDGCHC 1 cut(s) 360
MluCI AATT 4 cut(s) 33, 333, 437, 552
MlyI GAGTC 1 cut(s) 731
MmeI TCCRAC 2 cut(s) 499, 603
MnlI CCTC 7 cut(s) 347, 371, 476, 629, 727, 879, 964
Mph1103I ATGCAT 2 cut(s) 682, 727
MseI TTAA 3 cut(s) 144, 339, 663
MspA1I CMGCKG 2 cut(s) 796, 827
MspR9I CCNGG 1 cut(s) 879
MunI CAATTG 2 cut(s) 33, 552
Mva1269I GAATGC 1 cut(s) 219
MvaI CCWGG 1 cut(s) 879
MwoI GCNNNNNNNGC 1 cut(s) 941
NdeII GATC 4 cut(s) 65, 243, 289, 403
NlaIII CATG 8 cut(s) 259, 538, 680, 684, 755, 776, 823, 939
NlaIV GGNNCC 1 cut(s) 359
NmeAIII GCCGAG 1 cut(s) 840
NmuCI GTSAC 1 cut(s) 952
NsiI ATGCAT 2 cut(s) 682, 727
NspI RCATGY 4 cut(s) 538, 680, 823, 939
PaeI GCATGC 1 cut(s) 939
PaeR7I CTCGAG 1 cut(s) 351
PciI ACATGT 1 cut(s) 534
PctI GAATGC 1 cut(s) 219
PfeI GAWTC 5 cut(s) 193, 377, 639, 769, 799
PkrI GCNGC 3 cut(s) 218, 795, 829
PleI GAGTC 1 cut(s) 731
PpsI GAGTC 1 cut(s) 731
PscI ACATGT 1 cut(s) 534
PshBI ATTAAT 1 cut(s) 339
Psp6I CCWGG 1 cut(s) 877
PspGI CCWGG 1 cut(s) 877
PspN4I GGNNCC 1 cut(s) 359
PspXI VCTCGAGB 1 cut(s) 351
PstI CTGCAG 1 cut(s) 832
PsuI RGATCY 2 cut(s) 65, 403
PvuII CAGCTG 1 cut(s) 827
RsaI GTAC 1 cut(s) 82
RsaNI GTAC 1 cut(s) 81
SaqAI TTAA 3 cut(s) 144, 339, 663
SatI GCNGC 3 cut(s) 217, 794, 828
Sau3AI GATC 4 cut(s) 65, 243, 289, 403
SchI GAGTC 1 cut(s) 731
ScrFI CCNGG 1 cut(s) 879
SduI GDGCHC 1 cut(s) 360
SexAI ACCWGGT 1 cut(s) 877
SfaNI GCATC 5 cut(s) 203, 675, 712, 715, 734
SfcI CTRYAG 3 cut(s) 6, 586, 828
Sfr274I CTCGAG 1 cut(s) 351
SlaI CTCGAG 1 cut(s) 351
SmlI CTYRAG 1 cut(s) 351
SmoI CTYRAG 1 cut(s) 351
SpeI ACTAGT 1 cut(s) 964
SphI GCATGC 1 cut(s) 939
Sse9I AATT 4 cut(s) 33, 333, 437, 552
SsiI CCGC 1 cut(s) 796
SspMI CTAG 6 cut(s) 132, 197, 381, 471, 608, 965
StyD4I CCNGG 1 cut(s) 877
TaaI ACNGT 2 cut(s) 530, 890
TaiI ACGT 1 cut(s) 585
TaqI TCGA 6 cut(s) 288, 352, 728, 912, 969, 976
TaqII GACCGA 1 cut(s) 908
TasI AATT 4 cut(s) 33, 333, 437, 552
TfiI GAWTC 5 cut(s) 193, 377, 639, 769, 799
Tru1I TTAA 3 cut(s) 144, 339, 663
Tru9I TTAA 3 cut(s) 144, 339, 663
TscAI CASTG 2 cut(s) 114, 895
TseFI GTSAC 1 cut(s) 952
TseI GCWGC 3 cut(s) 216, 793, 827
Tsp45I GTSAC 1 cut(s) 952
TspDTI ATGAA 8 cut(s) 174, 244, 359, 386, 504, 761, 768, 963
TspRI CASTG 2 cut(s) 114, 895
VspI ATTAAT 1 cut(s) 339
XapI RAATTY 1 cut(s) 437
XbaI TCTAGA 2 cut(s) 131, 380
XceI RCATGY 4 cut(s) 538, 680, 823, 939
XhoI CTCGAG 1 cut(s) 351
XspI CTAG 6 cut(s) 132, 197, 381, 471, 608, 965
ZraI GACGTC 1 cut(s) 583
Zsp2I ATGCAT 2 cut(s) 682, 727
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.