Rh5CG202100

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000484
Physical Location & Seq
Forward (+)
20299465 .. 20315990
16526 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG202100.1

Sequence Viewer

Length: 2685 bp
ATGAAAGGGATAGAGAAAAGGATGGGACTTGACTTCAAGAATAATGCCAAAGAGTACCCAAATCAGAAATCTGCAGTTTTGTTAGAGTTGAGGGATTTGGATGACAAGATTTGTCCTGTGGAGTTTGGATTACGCATTCCTAATTGCCATCCGAAGTTGAATGGCATTGGAATGGGAGGCAGCACAAGTACATTTCAGTCCGTTAGAGATGGCCAGACTATAGTTTCAACTACTGGAAACTTTGAGCTTGGATTTTTTAGTCCTGCTACTGGTTCTAATAACCGATACCTTGGGATATGGTACAAGAAGATATCGGCTACCATAGTGTGGGTTGCCAACAGAAAAGCACCCCTTACTGATTCATCAGGTGTTCTGCAGGTCACCGACCCTGGAATTCTTGTCCTCCTCAACCATAACAAGAGCATAGTTTGGTCCTCAAACACATCAAGATCTGCAGAGAATCCAGTGGCCCAGCTTTTGGATTCAGGAAATCTTGTTGTAACAGACGGCAGTGATACAGACCCTGAGAACTTCTTGTGGCAAAGTTTTGATTACCCAAGTGATACACTCCTACCAGGTATGAAGCTTGGTTGGAACAAAGTTACTGGCTTCAGTAGGCATCTTACATCATGGAGCAGTCCACAGGATCCTTCTCTAGGAAAATATACATATGAACTTGATCGCAGAGGATATGGAGAAGAAATTTTGAAGAAGGATTCAGTTATGATATGGCGAACTGGACCATGGAATGGAATCCGATTCAGTGGCACGCCTCATTTAACTCCAAACTCCATATTCACATACAATCTTGTTTTTGATGATCATGATGAAGAATATTATATTTTTGAGCTTCTTAACAGCTCAGTTTATTCAAGATTGATCCTCACTCCAGATGGGCGTGTTCAGCGCTACACTTGGATTGATACAACCCAAACTTGGGGATTTCTTTATCAAACAGCTTTAATTGATAACTGCGACAGTTATGCATTCTGTGGTCCAAATGGTGCATGCAGTATTGACAGTTCCCCAGTATGTCGCTGCTTGAATGGATTTCAACCCAAAAATCCAAAGGACTGGGATGTAGTGGATTGGTCAAATGGCTGTGTGAGAAGGACTCCACTAAATTCAGTAAATTGCAGCGGTGACTTGTTTGAGAAGTACTCTGCAGTGAAATTGCCAAACACAGAGCAGTCCTGGTTCAACAGAAGTATGAATCTGAAGGAATGTGAAATGGTGTGCTTGAAGAACTGTTCCTGCACAGCTTATGCAAATTTGGATATCACGGATGGAGGATCTGGCTGCTTGCTGTGGTTTGGCAACCTAATTGATATTAGAAATTTAACTGTAAATGGGCAAGATATTTATATAAGAATGGCTGCATCAGAACTAGGTCTGGATCATGACAACACAGTTATCAATGCTAAAGACTCTGAATCTAAGAAGGTTAGAATCATACTATGTGGTACTCTGTTGCCTACTGGACTGCTGATCGTGGGCATAGGCCTGGTATTTTATTGCCGGAAGAAGCAGCCCAAAAAATATGGGAAAATGAGGAGCAGCAAAAAAGAAGATTCAGAACTTACCTCAAGAGAAGAAGATTTAGGACTTCCCTCAAAGAAAGAAGATTTTGAACTTCCATTATTTGACTTGGCTACTGTCGCTCTAAACATTGAAGGGCTTACAGCTGTATTAATCATCCTAAACCTTATAGCTCGAAGGGCATATCCTATTATCGTTGTACATTTCAAGCTTAATCCTAACATCAATGTACATTTTGCAACTTTCCAATCTCCTGAAAGCCTTCCAACACAACTTCCCGGCCGTCGCTATGCGTCCCCCAATCACCCTCCTACCAATTTAAGCCACATTCTGATCGGAATGGTTGGCTCCGTCAACACATTGAAGCACAGAACATCATACATTGAAGCATGGTGGCGGCCCAATGTCACTCGAGGATGCCTCTTTTTGGATCCTCCTACTGCAGCGTTTTTGCCTTGGCCTTCATCCTATCCTCCTTTTCGTGTCAATGAGGACATCACTAGATTAAAAGTGTACCCGAAAATTTTGAATACGGTCCAAGCACGCATAGTGCGTTCAATTTTGGAGATATTTACAGAAGGTGACAAAGATGCAAGATGGTCGCTGAAATCCTCCGGCTCCGATCCGGTGAAGCCGTTATCGGAGAGACCTTTGTCTAAGGAACCGGAGAAGGAGTTGTTGGAGAGGTCGAGTTTGTTTTTTGAATCATATAATGGCGGAGGAGTCACGGGAGTGGCGGTCGACAAGGGATTGGGAGTGGTGAGGGGAGTCAACTGGGCTGAGGATGGGGCTGGACTGAAAAGACGTGGTGGTCTTCTCGCCGTTGTGGGAGTCGCAGGAGAGGCTTTAGATGTAGTAGATTGGGCGACAGGGGAATCGGGCGATGACGACAATGACGACTTAAGTCAACTTGCTGCTATGGTGGTGGTTCCTGCTGCCGCCCAGCCCAAGGGTCTTCTTCAAGTGAAAAAGACTTTGCAGAAAGATTTGGGGAGAGAACCAAATAAGGGTGAACTAGCTGAGGCAACAAGTATGAGTGTGGTTCAAGTGAAAAAACACTTGGAGGTCGGTCAAGCTGCCATAAACAAAGTCGTCAAGGTTAAGGCATCAGATGATTTTATGTTGTTCAACTGCAGGGTAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

894

Amino Acids

99.17

Weight (kDa)

6.67

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 107 - 211 4e-40 D-mannose binding lectin
S_locus_glycop PF00954 244 - 355 2.9e-29 S-locus glycoprotein domain
PAN_2 PF08276 379 - 444 1e-20 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2349
Acc36I ACCTGC 1 cut(s) 367
AccB7I CCANNNNNTGG 3 cut(s) 327, 478, 749
AccI GTMKAC 2 cut(s) 2280, 2679
AciI CCGC 5 cut(s) 1140, 1936, 2256, 2276, 2478
AclWI GGATC 8 cut(s) 641, 654, 874, 1300, 1404, 1964, 1977, 2156
AcoI YGGCCR 2 cut(s) 211, 1819
AcsI RAATTY 6 cut(s) 393, 702, 1123, 1270, 1336, 2061
AcuI CTGAAG 2 cut(s) 595, 1238
AfaI GTAC 8 cut(s) 56, 190, 302, 1160, 1465, 1740, 1770, 2054
AfeI AGCGCT 1 cut(s) 908
AflII CTTAAG 1 cut(s) 2440
AjiI CACGTC 1 cut(s) 2345
AjnI CCWGG 4 cut(s) 388, 574, 1193, 1503
AjuI GAANNNNNNNTTGG 4 cut(s) 2201, 2233, 2582, 2614
AleI CACNNNNGTG 1 cut(s) 2270
Alw26I GTCTC 1 cut(s) 2179
AlwI GGATC 8 cut(s) 641, 654, 874, 1300, 1404, 1964, 1977, 2156
AlwNI CAGNNNCTG 1 cut(s) 524
Ama87I CYCGRG 1 cut(s) 1950
Aor51HI AGCGCT 1 cut(s) 908
AoxI GGCC 6 cut(s) 211, 468, 1501, 1819, 1937, 1997
ApoI RAATTY 6 cut(s) 393, 702, 1123, 1270, 1336, 2061
AseI ATTAAT 1 cut(s) 1691
Asp700I GAANNNNTTC 1 cut(s) 1800
AspLEI GCGC 1 cut(s) 909
AspS9I GGNCC 6 cut(s) 432, 469, 740, 995, 1938, 2074
AsuC2I CCSGG 1 cut(s) 1818
AsuHPI GGTGA 7 cut(s) 373, 1154, 1835, 2132, 2179, 2311, 2561
AvaI CYCGRG 1 cut(s) 1950
AvaII GGWCC 4 cut(s) 432, 740, 995, 2074
BalI TGGCCA 1 cut(s) 213
BamHI GGATCC 2 cut(s) 646, 1969
BbsI GAAGAC 2 cut(s) 2345, 2486
BbvCI CCTCAGC 2 cut(s) 2319, 2559
BccI CCATC 7 cut(s) 16, 156, 203, 887, 1280, 2130, 2318
BceAI ACGGC 4 cut(s) 523, 1806, 2158, 2345
BcgI CGANNNNNNTGC 4 cut(s) 965, 999, 2121, 2155
BciT130I CCWGG 4 cut(s) 390, 576, 1195, 1505
BclI TGATCA 1 cut(s) 820
BcnI CCSGG 1 cut(s) 1818
BcoDI GTCTC 1 cut(s) 2179
BfaI CTAG 4 cut(s) 656, 1388, 2040, 2555
BfmI CTRYAG 7 cut(s) 72, 219, 374, 453, 1164, 1980, 2671
BfoI RGCGCY 1 cut(s) 910
BfrI CTTAAG 1 cut(s) 2440
BfuAI ACCTGC 1 cut(s) 367
BglII AGATCT 1 cut(s) 449
BmcAI AGTACT 1 cut(s) 1160
Bme1390I CCNGG 5 cut(s) 390, 576, 1195, 1505, 1818
Bme18I GGWCC 4 cut(s) 432, 740, 995, 2074
BmeT110I CYCGRG 1 cut(s) 1950
BmgBI CACGTC 1 cut(s) 2345
BmgT120I GGNCC 6 cut(s) 432, 469, 740, 995, 1938, 2074
BmiI GGNNCC 6 cut(s) 648, 1888, 1971, 2158, 2202, 2469
BmrFI CCNGG 5 cut(s) 390, 576, 1195, 1505, 1818
BmrI ACTGGG 3 cut(s) 1022, 1084, 2323
BmsI GCATC 5 cut(s) 628, 1388, 1946, 2119, 2654
BmuI ACTGGG 3 cut(s) 1022, 1084, 2323
BoxI GACNNNNGTC 2 cut(s) 2191, 2442
BpiI GAAGAC 2 cut(s) 2345, 2486
BplI GAGNNNNNCTC 6 cut(s) 1099, 1131, 1145, 1177, 1944, 1976
BpmI CTGGAG 1 cut(s) 873
Bpu10I CCTNAGC 2 cut(s) 2319, 2559
BpuEI CTTGAG 1 cut(s) 1570
BpuMI CCSGG 1 cut(s) 1818
BsaI GGTCTC 1 cut(s) 2179
BsaJI CCNNGG 5 cut(s) 289, 388, 743, 1994, 2487
BsaWI WCCGGW 2 cut(s) 2164, 2203
BsaXI ACNNNNNCTCC 4 cut(s) 2198, 2228, 2262, 2292
Bse1I ACTGG 9 cut(s) 238, 274, 464, 610, 742, 1028, 1079, 1483, 2318
BseBI CCWGG 4 cut(s) 390, 576, 1195, 1505
BseDI CCNNGG 5 cut(s) 289, 388, 743, 1994, 2487
BseGI GGATG 9 cut(s) 27, 106, 148, 1084, 1291, 1695, 1961, 2003, 2329
BseMII CTCAG 4 cut(s) 516, 876, 2310, 2550
BseNI ACTGG 9 cut(s) 238, 274, 464, 610, 742, 1028, 1079, 1483, 2318
BseRI GAGGAG 3 cut(s) 395, 1567, 2274
BseX3I CGGCCG 1 cut(s) 1819
BseYI CCCAGC 2 cut(s) 471, 2481
BsgI GTGCAG 1 cut(s) 1240
Bsh1285I CGRYCG 2 cut(s) 1822, 2280
BshFI GGCC 6 cut(s) 213, 470, 1503, 1821, 1939, 1999
BsiEI CGRYCG 2 cut(s) 1822, 2280
BsiHKCI CYCGRG 1 cut(s) 1950
BsiSI CCGG 5 cut(s) 1519, 1818, 2154, 2165, 2204
BslFI GGGAC 2 cut(s) 39, 1819
BsmAI GTCTC 1 cut(s) 2179
BsmFI GGGAC 2 cut(s) 39, 1819
BsmI GAATGC 2 cut(s) 135, 986
BsnI GGCC 6 cut(s) 213, 470, 1503, 1821, 1939, 1999
Bso31I GGTCTC 1 cut(s) 2179
BsoBI CYCGRG 1 cut(s) 1950
Bsp1407I TGTACA 2 cut(s) 1738, 1768
Bsp19I CCATGG 1 cut(s) 743
BspACI CCGC 5 cut(s) 1140, 1936, 2256, 2276, 2478
BspANI GGCC 6 cut(s) 213, 470, 1503, 1821, 1939, 1999
BspCNI CTCAG 4 cut(s) 517, 875, 2311, 2551
BspHI TCATGA 2 cut(s) 823, 1399
BspLI GGNNCC 6 cut(s) 648, 1888, 1971, 2158, 2202, 2469
BspMAI CTGCAG 6 cut(s) 76, 378, 457, 1168, 1984, 2675
BspMI ACCTGC 1 cut(s) 367
BspPI GGATC 8 cut(s) 641, 654, 874, 1300, 1404, 1964, 1977, 2156
BspTI CTTAAG 1 cut(s) 2440
BspTNI GGTCTC 1 cut(s) 2179
BsrGI TGTACA 2 cut(s) 1738, 1768
BsrI ACTGG 9 cut(s) 238, 274, 464, 610, 742, 1028, 1079, 1483, 2318
BssECI CCNNGG 5 cut(s) 289, 388, 743, 1994, 2487
BssT1I CCWWGG 4 cut(s) 289, 743, 1994, 2487
Bst2UI CCWGG 4 cut(s) 390, 576, 1195, 1505
Bst4CI ACNGT 7 cut(s) 980, 1022, 1250, 1345, 1411, 1657, 2074
BstAFI CTTAAG 1 cut(s) 2440
BstAUI TGTACA 2 cut(s) 1738, 1768
BstC8I GCNNGC 4 cut(s) 770, 1009, 1304, 2083
BstDEI CTNAG 6 cut(s) 525, 862, 1437, 2196, 2319, 2559
BstDSI CCRYGG 1 cut(s) 743
BstEII GGTNACC 1 cut(s) 379
BstENI CCTNNNNNAGG 1 cut(s) 654
BstF5I GGATG 9 cut(s) 27, 106, 148, 1084, 1291, 1695, 1961, 2003, 2329
BstH2I RGCGCY 1 cut(s) 910
BstHHI GCGC 1 cut(s) 909
BstMAI GTCTC 1 cut(s) 2179
BstMCI CGRYCG 2 cut(s) 1822, 2280
BstMWI GCNNNNNNNGC 4 cut(s) 904, 1658, 1718, 2381
BstNI CCWGG 4 cut(s) 390, 576, 1195, 1505
BstNSI RCATGY 1 cut(s) 1011
BstPAI GACNNNNGTC 2 cut(s) 2191, 2442
BstPI GGTNACC 1 cut(s) 379
BstSCI CCNGG 5 cut(s) 388, 574, 1193, 1503, 1816
BstSFI CTRYAG 7 cut(s) 72, 219, 374, 453, 1164, 1980, 2671
BstV2I GAAGAC 2 cut(s) 2345, 2486
BstX2I RGATCY 4 cut(s) 449, 646, 1292, 1969
BstXI CCANNNNNNTGG 1 cut(s) 1074
BstYI RGATCY 4 cut(s) 449, 646, 1292, 1969
BstZI CGGCCG 1 cut(s) 1819
BsuRI GGCC 6 cut(s) 213, 470, 1503, 1821, 1939, 1999
BtgI CCRYGG 1 cut(s) 743
BtgZI GCGATG 1 cut(s) 2436
BtrI CACGTC 1 cut(s) 2345
BtsCI GGATG 9 cut(s) 27, 106, 148, 1084, 1291, 1695, 1961, 2003, 2329
BtsI GCAGTG 2 cut(s) 517, 1173
BtsIMutI CAGTG 4 cut(s) 471, 517, 769, 1173
BveI ACCTGC 1 cut(s) 367
Cac8I GCNNGC 4 cut(s) 770, 1009, 1304, 2083
CaiI CAGNNNCTG 1 cut(s) 524
CciI TCATGA 2 cut(s) 823, 1399
CfoI GCGC 1 cut(s) 909
Cfr13I GGNCC 6 cut(s) 432, 469, 740, 995, 1938, 2074
CseI GACGC 1 cut(s) 1821
CsiI ACCWGGT 1 cut(s) 574
Csp6I GTAC 8 cut(s) 55, 189, 301, 1159, 1464, 1739, 1769, 2053
CviAII CATG 6 cut(s) 630, 744, 824, 1008, 1400, 1929
CviQI GTAC 8 cut(s) 55, 189, 301, 1159, 1464, 1739, 1769, 2053
DdeI CTNAG 6 cut(s) 525, 862, 1437, 2196, 2319, 2559
DrdI GACNNNNNNGTC 1 cut(s) 2349
DseDI GACNNNNNNGTC 1 cut(s) 2349
EaeI YGGCCR 2 cut(s) 211, 1819
EagI CGGCCG 1 cut(s) 1819
EciI GGCGGA 1 cut(s) 2271
EclXI CGGCCG 1 cut(s) 1819
Eco130I CCWWGG 4 cut(s) 289, 743, 1994, 2487
Eco147I AGGCCT 1 cut(s) 1503
Eco31I GGTCTC 1 cut(s) 2179
Eco32I GATATC 2 cut(s) 312, 1279
Eco47I GGWCC 4 cut(s) 432, 740, 995, 2074
Eco47III AGCGCT 1 cut(s) 908
Eco52I CGGCCG 1 cut(s) 1819
Eco57I CTGAAG 2 cut(s) 595, 1238
Eco88I CYCGRG 1 cut(s) 1950
Eco91I GGTNACC 1 cut(s) 379
EcoNI CCTNNNNNAGG 1 cut(s) 654
EcoO65I GGTNACC 1 cut(s) 379
EcoRI GAATTC 1 cut(s) 393
EcoRII CCWGG 4 cut(s) 388, 574, 1193, 1503
EcoRV GATATC 2 cut(s) 312, 1279
EcoT14I CCWWGG 4 cut(s) 289, 743, 1994, 2487
EcoT22I ATGCAT 1 cut(s) 988
ErhI CCWWGG 4 cut(s) 289, 743, 1994, 2487
FaeI CATG 6 cut(s) 633, 747, 827, 1011, 1403, 1932
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FaqI GGGAC 2 cut(s) 39, 1819
FatI CATG 6 cut(s) 629, 743, 823, 1007, 1399, 1928
FauNDI CATATG 1 cut(s) 670
FbaI TGATCA 1 cut(s) 820
FblI GTMKAC 2 cut(s) 2280, 2679
FokI GGATG 9 cut(s) 34, 113, 135, 1091, 1298, 1682, 1968, 1990, 2336
FspBI CTAG 4 cut(s) 656, 1388, 2040, 2555
GlaI GCGC 1 cut(s) 908
GsaI CCCAGC 2 cut(s) 475, 2485
GsuI CTGGAG 1 cut(s) 873
HaeII RGCGCY 1 cut(s) 910
HaeIII GGCC 6 cut(s) 213, 470, 1503, 1821, 1939, 1999
HapII CCGG 5 cut(s) 1519, 1818, 2154, 2165, 2204
HgaI GACGC 1 cut(s) 1821
HhaI GCGC 1 cut(s) 909
Hin1II CATG 6 cut(s) 633, 747, 827, 1011, 1403, 1932
Hin6I GCGC 1 cut(s) 907
HinP1I GCGC 1 cut(s) 907
HincII GTYRAC 4 cut(s) 1894, 2281, 2311, 2447
HindII GTYRAC 4 cut(s) 1894, 2281, 2311, 2447
HindIII AAGCTT 2 cut(s) 584, 1748
HpaII CCGG 5 cut(s) 1519, 1818, 2154, 2165, 2204
HphI GGTGA 7 cut(s) 373, 1154, 1835, 2132, 2179, 2311, 2561
Hpy166II GTNNAC 8 cut(s) 641, 1894, 2053, 2281, 2311, 2447, 2552, 2680
Hpy8I GTNNAC 8 cut(s) 641, 1894, 2053, 2281, 2311, 2447, 2552, 2680
Hpy99I CGWCG 1 cut(s) 1827
HpyCH4III ACNGT 7 cut(s) 980, 1022, 1250, 1345, 1411, 1657, 2074
HpyCH4IV ACGT 1 cut(s) 2344
HpyF10VI GCNNNNNNNGC 4 cut(s) 904, 1658, 1718, 2381
HpyF3I CTNAG 6 cut(s) 525, 862, 1437, 2196, 2319, 2559
HpySE526I ACGT 1 cut(s) 2344
Hsp92II CATG 6 cut(s) 633, 747, 827, 1011, 1403, 1932
HspAI GCGC 1 cut(s) 907
Ksp22I TGATCA 1 cut(s) 820
LmnI GCTCC 4 cut(s) 633, 1554, 1892, 2162
LweI GCATC 5 cut(s) 628, 1388, 1946, 2119, 2654
MabI ACCWGGT 1 cut(s) 574
MaeI CTAG 4 cut(s) 656, 1388, 2040, 2555
MaeII ACGT 1 cut(s) 2344
MaeIII GTNAC 7 cut(s) 379, 499, 601, 1142, 1945, 2120, 2263
MflI RGATCY 4 cut(s) 449, 646, 1292, 1969
MlsI TGGCCA 1 cut(s) 213
MluNI TGGCCA 1 cut(s) 213
MlyI GAGTC 5 cut(s) 1108, 1421, 2271, 2316, 2379
MmeI TCCRAC 3 cut(s) 572, 1829, 2199
Mox20I TGGCCA 1 cut(s) 213
Mph1103I ATGCAT 1 cut(s) 988
MroXI GAANNNNTTC 1 cut(s) 1800
MscI TGGCCA 1 cut(s) 213
MslI CAYNNNNRTG 2 cut(s) 170, 2270
Msp20I TGGCCA 1 cut(s) 213
MspA1I CMGCKG 2 cut(s) 1140, 1685
MspCI CTTAAG 1 cut(s) 2440
MspI CCGG 5 cut(s) 1519, 1818, 2154, 2165, 2204
MspR9I CCNGG 5 cut(s) 390, 576, 1195, 1505, 1818
Mva1269I GAATGC 2 cut(s) 135, 986
MvaI CCWGG 4 cut(s) 390, 576, 1195, 1505
MwoI GCNNNNNNNGC 4 cut(s) 904, 1658, 1718, 2381
NciI CCSGG 1 cut(s) 1818
NcoI CCATGG 1 cut(s) 743
NdeI CATATG 1 cut(s) 670
NlaIII CATG 6 cut(s) 633, 747, 827, 1011, 1403, 1932
NlaIV GGNNCC 6 cut(s) 648, 1888, 1971, 2158, 2202, 2469
NmuCI GTSAC 5 cut(s) 379, 1142, 1945, 2120, 2263
NsiI ATGCAT 1 cut(s) 988
NspI RCATGY 1 cut(s) 1011
OliI CACNNNNGTG 1 cut(s) 2270
PaeI GCATGC 1 cut(s) 1011
PaeR7I CTCGAG 1 cut(s) 1950
PagI TCATGA 2 cut(s) 823, 1399
PceI AGGCCT 1 cut(s) 1503
PcsI WCGNNNNNNNCGW 2 cut(s) 2089, 2433
PctI GAATGC 2 cut(s) 135, 986
PdmI GAANNNNTTC 1 cut(s) 1800
PflMI CCANNNNNTGG 3 cut(s) 327, 478, 749
PleI GAGTC 5 cut(s) 1108, 1421, 2270, 2315, 2378
PpsI GAGTC 5 cut(s) 1108, 1421, 2270, 2315, 2378
PshAI GACNNNNGTC 2 cut(s) 2191, 2442
PshBI ATTAAT 1 cut(s) 1691
Psp6I CCWGG 4 cut(s) 388, 574, 1193, 1503
PspEI GGTNACC 1 cut(s) 379
PspFI CCCAGC 2 cut(s) 471, 2481
PspGI CCWGG 4 cut(s) 388, 574, 1193, 1503
PspN4I GGNNCC 6 cut(s) 648, 1888, 1971, 2158, 2202, 2469
PspPI GGNCC 6 cut(s) 432, 469, 740, 995, 1938, 2074
PspXI VCTCGAGB 1 cut(s) 1950
PsrI GAACNNNNNNTAC 2 cut(s) 1006, 1038
PstI CTGCAG 6 cut(s) 76, 378, 457, 1168, 1984, 2675
PstNI CAGNNNCTG 1 cut(s) 524
PsuI RGATCY 4 cut(s) 449, 646, 1292, 1969
PvuII CAGCTG 1 cut(s) 1685
RsaI GTAC 8 cut(s) 56, 190, 302, 1160, 1465, 1740, 1770, 2054
RsaNI GTAC 8 cut(s) 55, 189, 301, 1159, 1464, 1739, 1769, 2053
RseI CAYNNNNRTG 2 cut(s) 170, 2270
SalI GTCGAC 1 cut(s) 2279
Sau96I GGNCC 6 cut(s) 432, 469, 740, 995, 1938, 2074
ScaI AGTACT 1 cut(s) 1160
SchI GAGTC 5 cut(s) 1108, 1421, 2271, 2316, 2379
ScrFI CCNGG 5 cut(s) 390, 576, 1195, 1505, 1818
SexAI ACCWGGT 1 cut(s) 574
SfaNI GCATC 5 cut(s) 628, 1388, 1946, 2119, 2654
SfcI CTRYAG 7 cut(s) 72, 219, 374, 453, 1164, 1980, 2671
Sfr274I CTCGAG 1 cut(s) 1950
SinI GGWCC 4 cut(s) 432, 740, 995, 2074
SlaI CTCGAG 1 cut(s) 1950
SmiMI CAYNNNNRTG 2 cut(s) 170, 2270
SmlI CTYRAG 3 cut(s) 1585, 1950, 2440
SmoI CTYRAG 3 cut(s) 1585, 1950, 2440
SphI GCATGC 1 cut(s) 1011
SseBI AGGCCT 1 cut(s) 1503
SsiI CCGC 5 cut(s) 1140, 1936, 2256, 2276, 2478
SspI AATATT 1 cut(s) 836
SspMI CTAG 4 cut(s) 656, 1388, 2040, 2555
StuI AGGCCT 1 cut(s) 1503
StyD4I CCNGG 5 cut(s) 388, 574, 1193, 1503, 1816
StyI CCWWGG 4 cut(s) 289, 743, 1994, 2487
TaaI ACNGT 7 cut(s) 980, 1022, 1250, 1345, 1411, 1657, 2074
TaiI ACGT 1 cut(s) 2347
TaqI TCGA 4 cut(s) 1714, 1951, 2228, 2280
TaqII GACCGA 1 cut(s) 2597
TatI WGTACW 4 cut(s) 188, 1158, 1738, 1768
TauI GCSGC 2 cut(s) 1939, 2480
TscAI CASTG 4 cut(s) 471, 517, 769, 1173
TseFI GTSAC 5 cut(s) 379, 1142, 1945, 2120, 2263
Tsp45I GTSAC 5 cut(s) 379, 1142, 1945, 2120, 2263
TspDTI ATGAA 7 cut(s) 17, 351, 596, 687, 843, 1226, 1992
TspGWI ACGGA 3 cut(s) 190, 1298, 1879
TspRI CASTG 4 cut(s) 471, 517, 769, 1173
Van91I CCANNNNNTGG 3 cut(s) 327, 478, 749
Vha464I CTTAAG 1 cut(s) 2440
VpaK11BI GGWCC 4 cut(s) 432, 740, 995, 2074
VspI ATTAAT 1 cut(s) 1691
XagI CCTNNNNNAGG 1 cut(s) 654
XapI RAATTY 6 cut(s) 393, 702, 1123, 1270, 1336, 2061
XceI RCATGY 1 cut(s) 1011
XhoI CTCGAG 1 cut(s) 1950
XmiI GTMKAC 2 cut(s) 2280, 2679
XmnI GAANNNNTTC 1 cut(s) 1800
XspI CTAG 4 cut(s) 656, 1388, 2040, 2555
ZrmI AGTACT 1 cut(s) 1160
Zsp2I ATGCAT 1 cut(s) 988
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.