MD05G1233000.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000484
Physical Location & Seq
Forward (+)
36576148 .. 36579808
3661 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1233000.v1.1.491

Sequence Viewer

Length: 2472 bp
ATGAAAAATCCATTGAAAGCTTCAACCAAGATGTGCTTGCTTCTTTTCTACTCTACTTTGCTGCTCATTGTTGCAGCAGTATTCTCCATAGCTGATGACGGCACAAGTACATTTCAGTCCATTAGTGACGGTGAGACTGTAGTTTCAACCGGCGGAACATTTGAGCTAGGATTCTACAGTCCCGATGCTTCTAATAGACGGTACGTGGGGATATGGTACAAGAAGATTTCTGTTAAAACCATTGTATGGGTTGCCAACAGAGACACACCCCTCACTGATTTGTCTGGCGTCCTGAAGGTCACCAACCCCGGAATACTTGTCCTCAACCACAACATGAACACAATTTGGTCCTCCAATACATCGAGAACTGCACAGAATCCAGTGGCACGTCTTTTGGATTCGGGTAATCTTGTCGTGATAGATAGGAGTGATGATGACCCTGAAAACTTTCTGTGGCAAAGTTTTGATTACCCTGGCAATATAGTCTTACCAGGTGCGAAGCTTGGTAGGAACACAGTTACAGGCTTCAGTTGGCATGTTAGATCATGGAAAAGTCCTCAGGATCCTTCTCAAGGTAATTATACATATCAATTTGGTCCCAAAGGATATGCAGAAATGTTTCTGAGGGAAGGTTCGGTCATAAAATTTCGGAGTGGACCGTGGAATGGAGTCCGGTTCAGTGGAATGCCTCAGTTAAATCCAAACCCTATGTACACATACGGTCTTGTTTTTGAGCCTGATGAATTGTACTACAGTTACAAGCCTCGCAACAGCTCAATTCTTTCGAACTTGGTGTTAACTTCAGATGGACTTCTGCGGCGCTATACATGGATTGATAGAACCCAAAGTTGGGTTCTTTACCTAACAGTCCAGATCGATAACTGTGACAACTATGCATTCTGTGGTGTACATGGTACATGTAACATTGAAAAGTCCCCGGTTTGTAGCTGTTTGAAAGGATTTACACCGAAGTTTCCGAAAGAATGGGATTTGGTAGATTGGTCAAATGGTTGTGTGAGAAAGACTTCTCTAAATTGCACCGGAGACATGTTCCAAAAGTACTCGGGTTTGAAATTGCCTAGCACAGAACAATCCTGGTTTGACAGAAGTATGAACCTCAAGGAATGTGAGATGGTGTGCTTGAAGAACTGCTCCTGCACGGCGTATACAAATTTGGATATCCGAGATGGAGGAAGCGGATGTTTGCTATGGTACGGCGATCTAATTGATATAAGATACTTCGCTGAAAACGGGCAAGATATTTATATAAGAATGGCCTCATCGGAACTAGACAATACAAAGATCGACGCTAAATACTCTGAATCCAAAGCGAAGAAAATGAGAATCATAATAAGCACAATTGTGTTGTCTACCGGACTGTTGATCATGGGCCTGGCCCTGCTGTTTTATGTTTGGAAGAAGCAGCACCAGATAGGTGGAAAACTGGGATGCGGCCAAAAGGAGGATCTAGAATTACCGTTATTTGATTTGATGACTTTAGTTTCTGCAACCAGTAACTTTTCAATTGAAAACAAACTTGGTGAAGGCGGTTTCGGATCTGTCTTTAAGGGTACAATGAAAGATGGACAAGAAATCGCAGTGAAAAAGCTTTCAAAAAGTTCTCAACAAGGACTCAACGAGTTCAAGAATGAAGTTACACATATTGCCAAACTTCAGCACAGGAATCTAGTGAAGCTTCTCGGATACTGCATTCAAGAAGATGAGATGATGCTGATCTACGAGTTCATGCCTAACAAGAGCTTAGACTTCTTTATATTCGATCAAAGAAGAAGGATGTTGTTAGACTGGCCGAAGCGCTTTGAAATTATTAATGGGATAGCTCGAGGGATCCTCTATCTTCATCAAGATTCTAGACTGAGAGTTATCCATAGAGATCTCAAAGCAAGTAACATTTTGTTAGGCAGTGAATTTAATCCGAAAATCTCAGACTTTGGCCTGGCTAGAAGCTTTGGAGGAAATGAAACGAAAGCAAAAACGAAGAAAGTGGTCGGAACATACGGTTACATGTCCCCAGAATATGCAATTGATGGTTTCTACTCTATAAAGTCCGACGTCTATAGCTTTGGTGTTATGGTGTTAGAGATAGTGAGTGGGAGCAAAAATAGAGGATTCTCTCATCCAGACCACAAACTCAACCTTCTTGGACATGCGTGGATGCTACACACAGAAGGCAGGCCTCTCGAATTGCTTGATACATCGATAGAGGACTCCATCACTTTGCATGAAGTTGAACGAACGATTCTTGTGGGTCTTTTGTGCGTGCAGCAGAATCCAAAAGATAGGCCGAGCATGTCAGCTACAATTCTAATGTTGGGTGGTGAAGGTGCATTACCCTCACCCCTAAAACCGGGTTTCTACAGTGAAAGGGATCTGACTGAACTCGAAGCCAGTCATTCTTCTAAAGCATGCTCAACTAATGAAGTCACTATTTCACTACTCGAGGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

824

Amino Acids

92.53

Weight (kDa)

7.97

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 80 - 184 5.4e-35 D-mannose binding lectin
S_locus_glycop PF00954 216 - 325 3.6e-31 S-locus glycoprotein domain
PAN_2 PF08276 346 - 411 2.3e-21 PAN-like domain
PK_Tyr_Ser-Thr PF07714 508 - 773 2.3e-48 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 508 - 772 8.4e-46 Protein kinase domain
DUF3403 PF11883 782 - 823 2.8e-07 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2076
AccB7I CCANNNNNTGG 1 cut(s) 246
AccI GTMKAC 2 cut(s) 1166, 1370
AciI CCGC 5 cut(s) 153, 817, 1197, 1452, 1548
AclWI GGATC 7 cut(s) 557, 570, 1473, 1564, 1843, 1856, 2397
AcoI YGGCCR 2 cut(s) 1453, 1808
AcsI RAATTY 3 cut(s) 644, 1171, 1928
AcuI CTGAAG 4 cut(s) 314, 511, 786, 1658
AcyI GRCGYC 2 cut(s) 288, 2073
AfeI AGCGCT 1 cut(s) 1817
AfiI CCNNNNNNNGG 7 cut(s) 246, 572, 665, 849, 850, 1462, 2368
AflIII ACRYGT 3 cut(s) 917, 1047, 2025
AjiI CACGTC 1 cut(s) 389
AjnI CCWGG 5 cut(s) 472, 490, 1094, 1392, 1956
AjuI GAANNNNNNNTTGG 6 cut(s) 832, 837, 864, 869, 1521, 1553
AleI CACNNNNGTG 1 cut(s) 1361
Alw26I GTCTC 3 cut(s) 128, 255, 1038
AlwI GGATC 7 cut(s) 557, 570, 1473, 1564, 1843, 1856, 2397
Ama87I CYCGRG 3 cut(s) 1063, 1842, 2459
Aor51HI AGCGCT 1 cut(s) 1817
AoxI GGCC 8 cut(s) 1275, 1390, 1395, 1453, 1808, 1954, 2195, 2303
ApeKI GCWGC 4 cut(s) 61, 74, 1423, 2284
ApoI RAATTY 3 cut(s) 644, 1171, 1928
AseI ATTAAT 1 cut(s) 1830
Asp700I GAANNNNTTC 3 cut(s) 447, 618, 1024
AspLEI GCGC 2 cut(s) 822, 1818
AspS9I GGNCC 5 cut(s) 348, 596, 656, 1390, 1396
AsuC2I CCSGG 3 cut(s) 309, 938, 2370
AsuHPI GGTGA 5 cut(s) 143, 292, 1553, 2349, 2351
AsuII TTCGAA 1 cut(s) 785
AvaI CYCGRG 3 cut(s) 1063, 1842, 2459
AvaII GGWCC 3 cut(s) 348, 596, 656
AxyI CCTNAGG 1 cut(s) 558
BamHI GGATCC 2 cut(s) 562, 1848
BarI GAAGNNNNNNTAC 2 cut(s) 2334, 2366
BbvI GCAGC 4 cut(s) 48, 86, 1435, 2296
BccI CCATC 6 cut(s) 800, 1126, 1181, 1577, 2042, 2240
BceAI ACGGC 3 cut(s) 115, 1176, 1231
BcgI CGANNNNNNTGC 2 cut(s) 2182, 2216
BciT130I CCWGG 5 cut(s) 474, 492, 1096, 1394, 1958
BciVI GTATCC 1 cut(s) 1697
BclI TGATCA 1 cut(s) 1383
BcnI CCSGG 3 cut(s) 309, 938, 2370
BcoDI GTCTC 3 cut(s) 128, 255, 1038
BfaI CTAG 7 cut(s) 167, 1080, 1289, 1469, 1688, 1872, 1962
BfmI CTRYAG 5 cut(s) 138, 175, 751, 2077, 2377
BfoI RGCGCY 2 cut(s) 823, 1819
BfuI GTATCC 1 cut(s) 1697
BglII AGATCT 1 cut(s) 1894
BisI GCNGC 6 cut(s) 62, 75, 818, 1424, 1453, 2285
BlsI GCNGC 6 cut(s) 63, 76, 819, 1425, 1454, 2286
BmcAI AGTACT 1 cut(s) 1061
Bme1390I CCNGG 8 cut(s) 309, 474, 492, 938, 1096, 1394, 1958, 2370
Bme18I GGWCC 3 cut(s) 348, 596, 656
BmeT110I CYCGRG 3 cut(s) 1063, 1842, 2459
BmgBI CACGTC 1 cut(s) 389
BmgT120I GGNCC 5 cut(s) 348, 596, 656, 1390, 1396
BmiI GGNNCC 3 cut(s) 564, 598, 1850
BmrFI CCNGG 8 cut(s) 309, 474, 492, 938, 1096, 1394, 1958, 2370
BmrI ACTGGG 1 cut(s) 1454
BmsI GCATC 4 cut(s) 175, 1439, 1719, 2166
BmuI ACTGGG 1 cut(s) 1454
BplI GAGNNNNNCTC 2 cut(s) 1836, 1868
Bpu14I TTCGAA 1 cut(s) 785
BpuEI CTTGAG 2 cut(s) 555, 1103
BpuMI CCSGG 3 cut(s) 309, 938, 2370
Bsa29I ATCGAT 2 cut(s) 876, 2219
BsaAI YACGTR 1 cut(s) 205
BsaBI GATNNNNATC 1 cut(s) 1733
BsaHI GRCGYC 2 cut(s) 288, 2073
BsaJI CCNNGG 4 cut(s) 307, 472, 659, 936
BsaWI WCCGGW 3 cut(s) 672, 1040, 1373
Bsc4I CCNNNNNNNGG 7 cut(s) 246, 572, 665, 849, 850, 1462, 2368
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 5 cut(s) 380, 1449, 1512, 1811, 2409
Bse21I CCTNAGG 1 cut(s) 558
Bse8I GATNNNNATC 1 cut(s) 1733
BseBI CCWGG 5 cut(s) 474, 492, 1096, 1394, 1958
BseCI ATCGAT 2 cut(s) 876, 2219
BseDI CCNNGG 4 cut(s) 307, 472, 659, 936
BseGI GGATG 5 cut(s) 1205, 1454, 1800, 2137, 2181
BseJI GATNNNNATC 1 cut(s) 1733
BseLI CCNNNNNNNGG 7 cut(s) 246, 572, 665, 849, 850, 1462, 2368
BseMII CTCAG 5 cut(s) 572, 614, 704, 1868, 1959
BseNI ACTGG 5 cut(s) 380, 1449, 1512, 1811, 2409
BseXI GCAGC 4 cut(s) 48, 86, 1435, 2296
BsgI GTGCAG 3 cut(s) 354, 1141, 2303
BshFI GGCC 8 cut(s) 1277, 1392, 1397, 1455, 1810, 1956, 2197, 2305
BshVI ATCGAT 2 cut(s) 876, 2219
BsiHKCI CYCGRG 3 cut(s) 1063, 1842, 2459
BsiSI CCGG 7 cut(s) 150, 309, 673, 938, 1041, 1374, 2369
BslFI GGGAC 4 cut(s) 165, 582, 919, 2014
BslI CCNNNNNNNGG 7 cut(s) 246, 572, 665, 849, 850, 1462, 2368
BsmAI GTCTC 3 cut(s) 128, 255, 1038
BsmFI GGGAC 4 cut(s) 165, 582, 919, 2014
BsmI GAATGC 3 cut(s) 690, 896, 1710
BsnI GGCC 8 cut(s) 1277, 1392, 1397, 1455, 1810, 1956, 2197, 2305
BsoBI CYCGRG 3 cut(s) 1063, 1842, 2459
Bsp119I TTCGAA 1 cut(s) 785
Bsp1407I TGTACA 2 cut(s) 711, 907
BspACI CCGC 5 cut(s) 153, 817, 1197, 1452, 1548
BspANI GGCC 8 cut(s) 1277, 1392, 1397, 1455, 1810, 1956, 2197, 2305
BspCNI CTCAG 5 cut(s) 571, 615, 703, 1869, 1958
BspDI ATCGAT 2 cut(s) 876, 2219
BspLI GGNNCC 3 cut(s) 564, 598, 1850
BspPI GGATC 7 cut(s) 557, 570, 1473, 1564, 1843, 1856, 2397
BspT104I TTCGAA 1 cut(s) 785
BsrFI RCCGGY 1 cut(s) 149
BsrGI TGTACA 2 cut(s) 711, 907
BsrI ACTGG 5 cut(s) 380, 1449, 1512, 1811, 2409
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 4 cut(s) 307, 472, 659, 936
BssNAI GTATAC 1 cut(s) 1167
BssNI GRCGYC 2 cut(s) 288, 2073
Bst1107I GTATAC 1 cut(s) 1167
Bst2UI CCWGG 5 cut(s) 474, 492, 1096, 1394, 1958
BstACI GRCGYC 2 cut(s) 288, 2073
BstAUI TGTACA 2 cut(s) 711, 907
BstBAI YACGTR 1 cut(s) 205
BstBI TTCGAA 1 cut(s) 785
BstC8I GCNNGC 4 cut(s) 38, 2195, 2282, 2428
BstDEI CTNAG 6 cut(s) 558, 623, 690, 1762, 1877, 1945
BstDSI CCRYGG 1 cut(s) 659
BstEII GGTNACC 1 cut(s) 298
BstENI CCTNNNNNAGG 1 cut(s) 570
BstF5I GGATG 5 cut(s) 1205, 1454, 1800, 2137, 2181
BstH2I RGCGCY 2 cut(s) 823, 1819
BstHHI GCGC 2 cut(s) 822, 1818
BstMAI GTCTC 3 cut(s) 128, 255, 1038
BstNI CCWGG 5 cut(s) 474, 492, 1096, 1394, 1958
BstNSI RCATGY 7 cut(s) 539, 921, 1051, 2029, 2171, 2314, 2430
BstPI GGTNACC 1 cut(s) 298
BstSCI CCNGG 8 cut(s) 307, 472, 490, 936, 1094, 1392, 1956, 2368
BstSFI CTRYAG 5 cut(s) 138, 175, 751, 2077, 2377
BstV1I GCAGC 4 cut(s) 48, 86, 1435, 2296
BstX2I RGATCY 6 cut(s) 562, 1465, 1556, 1848, 1894, 2389
BstXI CCANNNNNNTGG 1 cut(s) 1436
BstYI RGATCY 6 cut(s) 562, 1465, 1556, 1848, 1894, 2389
BstZ17I GTATAC 1 cut(s) 1167
Bsu15I ATCGAT 2 cut(s) 876, 2219
Bsu36I CCTNAGG 1 cut(s) 558
BsuI GTATCC 1 cut(s) 1697
BsuRI GGCC 8 cut(s) 1277, 1392, 1397, 1455, 1810, 1956, 2197, 2305
BsuTUI ATCGAT 2 cut(s) 876, 2219
BtgI CCRYGG 1 cut(s) 659
BtrI CACGTC 1 cut(s) 389
BtsCI GGATG 5 cut(s) 1205, 1454, 1800, 2137, 2181
BtsI GCAGTG 2 cut(s) 1605, 1930
BtsIMutI CAGTG 6 cut(s) 273, 387, 685, 1605, 1930, 2386
Cac8I GCNNGC 4 cut(s) 38, 2195, 2282, 2428
CfoI GCGC 2 cut(s) 822, 1818
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 5 cut(s) 348, 596, 656, 1390, 1396
ClaI ATCGAT 2 cut(s) 876, 2219
CseI GACGC 2 cut(s) 277, 1316
CsiI ACCWGGT 1 cut(s) 490
DdeI CTNAG 6 cut(s) 558, 623, 690, 1762, 1877, 1945
EaeI YGGCCR 2 cut(s) 1453, 1808
EciI GGCGGA 1 cut(s) 168
Eco147I AGGCCT 1 cut(s) 2197
Eco32I GATATC 1 cut(s) 1180
Eco47I GGWCC 3 cut(s) 348, 596, 656
Eco47III AGCGCT 1 cut(s) 1817
Eco57I CTGAAG 4 cut(s) 314, 511, 786, 1658
Eco81I CCTNAGG 1 cut(s) 558
Eco88I CYCGRG 3 cut(s) 1063, 1842, 2459
Eco91I GGTNACC 1 cut(s) 298
EcoNI CCTNNNNNAGG 1 cut(s) 570
EcoO65I GGTNACC 1 cut(s) 298
EcoRII CCWGG 5 cut(s) 472, 490, 1094, 1392, 1956
EcoRV GATATC 1 cut(s) 1180
EcoT22I ATGCAT 1 cut(s) 898
FalI AAGNNNNNCTT 2 cut(s) 20, 52
FaqI GGGAC 4 cut(s) 165, 582, 919, 2014
FbaI TGATCA 1 cut(s) 1383
FblI GTMKAC 2 cut(s) 1166, 1370
Fnu4HI GCNGC 6 cut(s) 62, 75, 818, 1424, 1453, 2285
FokI GGATG 5 cut(s) 1212, 1461, 1807, 2124, 2188
Fsp4HI GCNGC 6 cut(s) 62, 75, 818, 1424, 1453, 2285
FspBI CTAG 7 cut(s) 167, 1080, 1289, 1469, 1688, 1872, 1962
GlaI GCGC 2 cut(s) 821, 1817
GluI GCNGC 6 cut(s) 62, 75, 818, 1424, 1453, 2285
HaeII RGCGCY 2 cut(s) 823, 1819
HaeIII GGCC 8 cut(s) 1277, 1392, 1397, 1455, 1810, 1956, 2197, 2305
HapII CCGG 7 cut(s) 150, 309, 673, 938, 1041, 1374, 2369
HgaI GACGC 2 cut(s) 277, 1316
HhaI GCGC 2 cut(s) 822, 1818
Hin1I GRCGYC 2 cut(s) 288, 2073
Hin6I GCGC 2 cut(s) 820, 1816
HinP1I GCGC 2 cut(s) 820, 1816
HincII GTYRAC 1 cut(s) 798
HindII GTYRAC 1 cut(s) 798
HindIII AAGCTT 5 cut(s) 18, 500, 1607, 1694, 1966
HpaI GTTAAC 1 cut(s) 798
HpaII CCGG 7 cut(s) 150, 309, 673, 938, 1041, 1374, 2369
HphI GGTGA 5 cut(s) 143, 292, 1553, 2349, 2351
Hpy166II GTNNAC 6 cut(s) 656, 714, 798, 908, 1167, 1371
Hpy8I GTNNAC 6 cut(s) 656, 714, 798, 908, 1167, 1371
Hpy99I CGWCG 2 cut(s) 1310, 2075
HpyAV CCTTC 8 cut(s) 289, 576, 623, 1538, 1785, 2168, 2183, 2336
HpyCH4IV ACGT 3 cut(s) 204, 388, 2073
HpyF3I CTNAG 6 cut(s) 558, 623, 690, 1762, 1877, 1945
HpySE526I ACGT 3 cut(s) 204, 388, 2073
Hsp92I GRCGYC 2 cut(s) 288, 2073
HspAI GCGC 2 cut(s) 820, 1816
Ksp22I TGATCA 1 cut(s) 1383
KspAI GTTAAC 1 cut(s) 798
LmnI GCTCC 2 cut(s) 1157, 2115
Lsp1109I GCAGC 4 cut(s) 48, 86, 1435, 2296
LweI GCATC 4 cut(s) 175, 1439, 1719, 2166
MabI ACCWGGT 1 cut(s) 490
MaeI CTAG 7 cut(s) 167, 1080, 1289, 1469, 1688, 1872, 1962
MaeII ACGT 3 cut(s) 204, 388, 2073
MboII GAAGA 9 cut(s) 235, 1156, 1345, 1429, 1730, 1800, 1850, 2011, 2409
MfeI CAATTG 3 cut(s) 1359, 1524, 2043
MflI RGATCY 6 cut(s) 562, 1465, 1556, 1848, 1894, 2389
MlyI GAGTC 3 cut(s) 678, 1626, 2222
MmeI TCCRAC 2 cut(s) 1990, 2094
Mph1103I ATGCAT 1 cut(s) 898
MroXI GAANNNNTTC 3 cut(s) 447, 618, 1024
MseI TTAA 6 cut(s) 234, 695, 797, 1566, 1830, 1932
MslI CAYNNNNRTG 1 cut(s) 1361
MspI CCGG 7 cut(s) 150, 309, 673, 938, 1041, 1374, 2369
MspR9I CCNGG 8 cut(s) 309, 474, 492, 938, 1096, 1394, 1958, 2370
MunI CAATTG 3 cut(s) 1359, 1524, 2043
Mva1269I GAATGC 3 cut(s) 690, 896, 1710
MvaI CCWGG 5 cut(s) 474, 492, 1096, 1394, 1958
NciI CCSGG 3 cut(s) 309, 938, 2370
NlaIV GGNNCC 3 cut(s) 564, 598, 1850
NmeAIII GCCGAG 1 cut(s) 2331
NmuCI GTSAC 4 cut(s) 125, 298, 884, 2443
NsiI ATGCAT 1 cut(s) 898
NspI RCATGY 7 cut(s) 539, 921, 1051, 2029, 2171, 2314, 2430
NspV TTCGAA 1 cut(s) 785
OliI CACNNNNGTG 1 cut(s) 1361
PaeI GCATGC 1 cut(s) 2430
PaeR7I CTCGAG 2 cut(s) 1842, 2459
PceI AGGCCT 1 cut(s) 2197
PciI ACATGT 3 cut(s) 917, 1047, 2025
PctI GAATGC 3 cut(s) 690, 896, 1710
PdmI GAANNNNTTC 3 cut(s) 447, 618, 1024
PflMI CCANNNNNTGG 1 cut(s) 246
PkrI GCNGC 6 cut(s) 63, 76, 819, 1425, 1454, 2286
PleI GAGTC 3 cut(s) 677, 1626, 2222
PpsI GAGTC 3 cut(s) 677, 1626, 2222
Ppu21I YACGTR 1 cut(s) 205
PscI ACATGT 3 cut(s) 917, 1047, 2025
PshBI ATTAAT 1 cut(s) 1830
Psp6I CCWGG 5 cut(s) 472, 490, 1094, 1392, 1956
PspEI GGTNACC 1 cut(s) 298
PspGI CCWGG 5 cut(s) 472, 490, 1094, 1392, 1956
PspN4I GGNNCC 3 cut(s) 564, 598, 1850
PspPI GGNCC 5 cut(s) 348, 596, 656, 1390, 1396
PspXI VCTCGAGB 2 cut(s) 1842, 2459
PsuI RGATCY 6 cut(s) 562, 1465, 1556, 1848, 1894, 2389
RseI CAYNNNNRTG 1 cut(s) 1361
SaqAI TTAA 6 cut(s) 234, 695, 797, 1566, 1830, 1932
SatI GCNGC 6 cut(s) 62, 75, 818, 1424, 1453, 2285
Sau96I GGNCC 5 cut(s) 348, 596, 656, 1390, 1396
ScaI AGTACT 1 cut(s) 1061
SchI GAGTC 3 cut(s) 678, 1626, 2222
ScrFI CCNGG 8 cut(s) 309, 474, 492, 938, 1096, 1394, 1958, 2370
SexAI ACCWGGT 1 cut(s) 490
SfaNI GCATC 4 cut(s) 175, 1439, 1719, 2166
SfcI CTRYAG 5 cut(s) 138, 175, 751, 2077, 2377
Sfr274I CTCGAG 2 cut(s) 1842, 2459
SfuI TTCGAA 1 cut(s) 785
SinI GGWCC 3 cut(s) 348, 596, 656
SlaI CTCGAG 2 cut(s) 1842, 2459
SmiMI CAYNNNNRTG 1 cut(s) 1361
SmlI CTYRAG 4 cut(s) 570, 1118, 1842, 2459
SmoI CTYRAG 4 cut(s) 570, 1118, 1842, 2459
SphI GCATGC 1 cut(s) 2430
SseBI AGGCCT 1 cut(s) 2197
SsiI CCGC 5 cut(s) 153, 817, 1197, 1452, 1548
SspMI CTAG 7 cut(s) 167, 1080, 1289, 1469, 1688, 1872, 1962
StuI AGGCCT 1 cut(s) 2197
StyD4I CCNGG 8 cut(s) 307, 472, 490, 936, 1094, 1392, 1956, 2368
TaiI ACGT 3 cut(s) 207, 391, 2076
TaqII GACCGA 1 cut(s) 625
TatI WGTACW 5 cut(s) 107, 711, 747, 907, 1059
TauI GCSGC 2 cut(s) 820, 1455
Tru1I TTAA 6 cut(s) 234, 695, 797, 1566, 1830, 1932
Tru9I TTAA 6 cut(s) 234, 695, 797, 1566, 1830, 1932
TscAI CASTG 6 cut(s) 280, 387, 685, 1605, 1930, 2386
TseFI GTSAC 4 cut(s) 125, 298, 884, 2443
TseI GCWGC 4 cut(s) 61, 74, 1423, 2284
Tsp45I GTSAC 4 cut(s) 125, 298, 884, 2443
TspRI CASTG 6 cut(s) 280, 387, 685, 1605, 1930, 2386
Van91I CCANNNNNTGG 1 cut(s) 246
VpaK11BI GGWCC 3 cut(s) 348, 596, 656
VspI ATTAAT 1 cut(s) 1830
XagI CCTNNNNNAGG 1 cut(s) 570
XapI RAATTY 3 cut(s) 644, 1171, 1928
XbaI TCTAGA 2 cut(s) 1468, 1871
XceI RCATGY 7 cut(s) 539, 921, 1051, 2029, 2171, 2314, 2430
XhoI CTCGAG 2 cut(s) 1842, 2459
XmiI GTMKAC 2 cut(s) 1166, 1370
XmnI GAANNNNTTC 3 cut(s) 447, 618, 1024
XspI CTAG 7 cut(s) 167, 1080, 1289, 1469, 1688, 1872, 1962
ZraI GACGTC 1 cut(s) 2074
ZrmI AGTACT 1 cut(s) 1061
Zsp2I ATGCAT 1 cut(s) 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.