Rh5AG186300

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000484
Physical Location & Seq
Forward (+)
21350859 .. 21351218
360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG186300.1

Sequence Viewer

Length: 360 bp
ATGAAGCTTGGTAGGAACACAGTTACAGGCTTCAATTGGCATCTTACATTGTGGAGAAGTCCCTCTGATCCTTCTCAAGGCAACTTCACATTTCAACATGGTCCCAGAGGATATGCAGAACAAGTTCTGAGGGCGGGTTCTGACATAAGATTTCGGACCGGACGGTGGAATGGAATCCGATTCAGTGGAACGCCTCATTTAGGTCCAAACCATGTTTACACATATCAGCTAGTGTTTGATTATCATGATCATGAAGAAAACTATACCTATAAGCTTCTCAACAGCTCAGTTCTTTCCAGGCTGGTAACTCAAGATGGGCTTCTGCAGCGCTACACATGGATTGATAGAACCCAAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

119

Amino Acids

13.93

Weight (kDa)

9.69

Isoelectric Point (pI)

34.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S_locus_glycop PF00954 51 - 118 3.3e-09 S-locus glycoprotein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 134
AclWI GGATC 1 cut(s) 62
AfeI AGCGCT 1 cut(s) 329
AfiI CCNNNNNNNGG 3 cut(s) 77, 165, 200
AgsI TTSAA 2 cut(s) 34, 95
AjnI CCWGG 1 cut(s) 296
AluBI AGCT 4 cut(s) 7, 229, 274, 285
AluI AGCT 4 cut(s) 7, 229, 274, 285
AlwI GGATC 1 cut(s) 62
Aor51HI AGCGCT 1 cut(s) 329
ApeKI GCWGC 1 cut(s) 325
Asp700I GAANNNNTTC 1 cut(s) 123
AspLEI GCGC 1 cut(s) 330
AspS9I GGNCC 3 cut(s) 101, 156, 203
AvaII GGWCC 3 cut(s) 101, 156, 203
BbvI GCAGC 1 cut(s) 337
BccI CCATC 1 cut(s) 308
BciT130I CCWGG 1 cut(s) 298
BclI TGATCA 1 cut(s) 247
BfaI CTAG 1 cut(s) 230
BfmI CTRYAG 1 cut(s) 323
BfoI RGCGCY 1 cut(s) 331
BisI GCNGC 1 cut(s) 326
BlsI GCNGC 1 cut(s) 327
Bme1390I CCNGG 1 cut(s) 298
Bme18I GGWCC 3 cut(s) 101, 156, 203
BmgT120I GGNCC 3 cut(s) 101, 156, 203
BmiI GGNNCC 1 cut(s) 103
BmrFI CCNGG 1 cut(s) 298
BmsI GCATC 1 cut(s) 49
BpuEI CTTGAG 2 cut(s) 60, 294
BsaWI WCCGGW 1 cut(s) 158
Bsc4I CCNNNNNNNGG 3 cut(s) 77, 165, 200
BseBI CCWGG 1 cut(s) 298
BseLI CCNNNNNNNGG 3 cut(s) 77, 165, 200
BseMII CTCAG 2 cut(s) 119, 300
BseXI GCAGC 1 cut(s) 337
BsiSI CCGG 1 cut(s) 159
BslFI GGGAC 2 cut(s) 45, 87
BslI CCNNNNNNNGG 3 cut(s) 77, 165, 200
BsmFI GGGAC 2 cut(s) 45, 87
Bsp143I GATC 2 cut(s) 67, 247
BspACI CCGC 1 cut(s) 134
BspCNI CTCAG 2 cut(s) 120, 299
BspHI TCATGA 2 cut(s) 244, 250
BspLI GGNNCC 1 cut(s) 103
BspMAI CTGCAG 1 cut(s) 327
BspPI GGATC 1 cut(s) 62
BssMI GATC 2 cut(s) 67, 247
Bst2UI CCWGG 1 cut(s) 298
Bst4CI ACNGT 2 cut(s) 22, 165
BstDEI CTNAG 2 cut(s) 128, 286
BstENI CCTNNNNNAGG 2 cut(s) 75, 198
BstH2I RGCGCY 1 cut(s) 331
BstHHI GCGC 1 cut(s) 330
BstKTI GATC 2 cut(s) 70, 250
BstMBI GATC 2 cut(s) 67, 247
BstMWI GCNNNNNNNGC 1 cut(s) 325
BstNI CCWGG 1 cut(s) 298
BstSCI CCNGG 1 cut(s) 296
BstSFI CTRYAG 1 cut(s) 323
BstV1I GCAGC 1 cut(s) 337
BtsIMutI CAGTG 1 cut(s) 190
CciI TCATGA 2 cut(s) 244, 250
CfoI GCGC 1 cut(s) 330
Cfr13I GGNCC 3 cut(s) 101, 156, 203
CpoI CGGWCCG 1 cut(s) 156
CspI CGGWCCG 1 cut(s) 156
CviAII CATG 5 cut(s) 98, 212, 245, 251, 336
CviJI RGCY 7 cut(s) 7, 30, 229, 274, 285, 301, 319
CviKI_1 RGCY 7 cut(s) 7, 30, 229, 274, 285, 301, 319
DdeI CTNAG 2 cut(s) 128, 286
DpnI GATC 2 cut(s) 69, 249
DpnII GATC 2 cut(s) 67, 247
Eco47I GGWCC 3 cut(s) 101, 156, 203
Eco47III AGCGCT 1 cut(s) 329
EcoNI CCTNNNNNAGG 2 cut(s) 75, 198
EcoRII CCWGG 1 cut(s) 296
FaeI CATG 5 cut(s) 101, 215, 248, 254, 339
FalI AAGNNNNNCTT 2 cut(s) 303, 335
FaqI GGGAC 2 cut(s) 45, 87
FatI CATG 5 cut(s) 97, 211, 244, 250, 335
FauI CCCGC 1 cut(s) 127
FbaI TGATCA 1 cut(s) 247
Fnu4HI GCNGC 1 cut(s) 326
Fsp4HI GCNGC 1 cut(s) 326
FspBI CTAG 1 cut(s) 230
GlaI GCGC 1 cut(s) 329
GluI GCNGC 1 cut(s) 326
HaeII RGCGCY 1 cut(s) 331
HapII CCGG 1 cut(s) 159
HhaI GCGC 1 cut(s) 330
Hin1II CATG 5 cut(s) 101, 215, 248, 254, 339
Hin6I GCGC 1 cut(s) 328
HinP1I GCGC 1 cut(s) 328
HindIII AAGCTT 2 cut(s) 5, 272
HinfI GANTC 2 cut(s) 174, 180
HpaII CCGG 1 cut(s) 159
Hpy166II GTNNAC 1 cut(s) 217
Hpy188I TCNGA 5 cut(s) 67, 129, 142, 156, 179
Hpy188III TCNNGA 3 cut(s) 245, 251, 311
Hpy8I GTNNAC 1 cut(s) 217
HpyAV CCTTC 1 cut(s) 81
HpyCH4III ACNGT 2 cut(s) 22, 165
HpyCH4V TGCA 2 cut(s) 116, 325
HpyF10VI GCNNNNNNNGC 1 cut(s) 325
HpyF3I CTNAG 2 cut(s) 128, 286
Hsp92II CATG 5 cut(s) 101, 215, 248, 254, 339
HspAI GCGC 1 cut(s) 328
Ksp22I TGATCA 1 cut(s) 247
Kzo9I GATC 2 cut(s) 67, 247
LpnPI CCDG 6 cut(s) 12, 118, 172, 283, 287, 310
Lsp1109I GCAGC 1 cut(s) 337
LweI GCATC 1 cut(s) 49
MaeI CTAG 1 cut(s) 230
MaeIII GTNAC 2 cut(s) 22, 304
MalI GATC 2 cut(s) 69, 249
MboI GATC 2 cut(s) 67, 247
MboII GAAGA 1 cut(s) 266
MfeI CAATTG 1 cut(s) 34
MluCI AATT 1 cut(s) 34
MnlI CCTC 4 cut(s) 73, 101, 123, 204
MroXI GAANNNNTTC 1 cut(s) 123
MslI CAYNNNNRTG 1 cut(s) 249
MspI CCGG 1 cut(s) 159
MspR9I CCNGG 1 cut(s) 298
MunI CAATTG 1 cut(s) 34
MvaI CCWGG 1 cut(s) 298
MwoI GCNNNNNNNGC 1 cut(s) 325
NdeII GATC 2 cut(s) 67, 247
NlaIII CATG 5 cut(s) 101, 215, 248, 254, 339
NlaIV GGNNCC 1 cut(s) 103
PagI TCATGA 2 cut(s) 244, 250
PdmI GAANNNNTTC 1 cut(s) 123
PfeI GAWTC 2 cut(s) 174, 180
PkrI GCNGC 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 296
PspGI CCWGG 1 cut(s) 296
PspN4I GGNNCC 1 cut(s) 103
PspPI GGNCC 3 cut(s) 101, 156, 203
PstI CTGCAG 1 cut(s) 327
RseI CAYNNNNRTG 1 cut(s) 249
Rsr2I CGGWCCG 1 cut(s) 156
RsrII CGGWCCG 1 cut(s) 156
SatI GCNGC 1 cut(s) 326
Sau3AI GATC 2 cut(s) 67, 247
Sau96I GGNCC 3 cut(s) 101, 156, 203
ScrFI CCNGG 1 cut(s) 298
SetI ASST 6 cut(s) 9, 205, 231, 269, 276, 287
SfaNI GCATC 1 cut(s) 49
SfcI CTRYAG 1 cut(s) 323
SinI GGWCC 3 cut(s) 101, 156, 203
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 2 cut(s) 75, 309
SmoI CTYRAG 2 cut(s) 75, 309
Sse9I AATT 1 cut(s) 34
SsiI CCGC 1 cut(s) 134
SspMI CTAG 1 cut(s) 230
StyD4I CCNGG 1 cut(s) 296
TaaI ACNGT 2 cut(s) 22, 165
TasI AATT 1 cut(s) 34
TfiI GAWTC 2 cut(s) 174, 180
TscAI CASTG 1 cut(s) 190
TseI GCWGC 1 cut(s) 325
TspDTI ATGAA 2 cut(s) 17, 267
TspRI CASTG 1 cut(s) 190
VpaK11BI GGWCC 3 cut(s) 101, 156, 203
XagI CCTNNNNNAGG 2 cut(s) 75, 198
XmnI GAANNNNTTC 1 cut(s) 123
XspI CTAG 1 cut(s) 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.