RLG00000032923

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000484
Physical Location & Seq
Forward (+)
20907797 .. 20909488
1692 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032923

Sequence Viewer

Length: 1347 bp
ATGAAAAATCCATTGAAGGTTAGGATGTGCTTACATCCTATTTGCTCTTTTTTGCTTATCATCACAGCGATATTTTCCACAGCGGGAGGCAGCACAAGTACATTTCAGTCCATTAGAGATGGTCAGACTATAGTTTCAACTACTGGAAACTTTGAGCTTGGATTTTTTAGTCCTTCTACTGGTTCTAATAATCGATATGTTGGGATATGGTACAAGAAGATATCGTCTACAATAGTATGGGTTGCCAATAGAGAAGCACCCCTTACTGATTCATTAGGTGTTCTGCAGGCCACCAACCCTGGAATTCTTGTCCTCCTCAACCATACCAAGAGCATAGTTTGGTCCTCAAACACATCAAGATCTGCAGAGAATCCAGTGGCCCAGCTTTTGGATTCAGGAAATCTTGTTGTAACAAATGGCAGTGATACAGAGCCTGAGAACTTCTTGTGGCAAAGTTTTGATTACCCAAGTGATACACTCCTACCAGGTATGAAGCTTGGTTGGAACAAAGTTACAGGCTTCAGTAGGCATCTTACATCATGGAATAGTCCACAGGATCCTTCTCTAGGAAAATATACATATGAACTTGATCGCAGAGGATATGGAGAAGAAATTTTGAAGAAGGAGTCAGTTATGATATGGAGAACTGGACCATGGAATGGAATCCGATTTAGTGGCTTGCCTCATTTAACTCCAAACTCCGTATTCAAATGTAATTTTGTTTTTGATGATCATGATGAAGAATATTATATTTTTGAGCTTTATAACAGCTCAGTTTATTCAAGAGTGAGTGTCACTTCAGAAGGGCGTGTGCAGCGCTACACATGGATTGATACAACCCAAACTTTGGGATTCCTTTATCAAACAGTTATTGATAACTGCGAAAGTTATGCATTCTGTGGTCCAAATGGTGCATGCAATATTGACAGTTCCCCAGTATGTCGCTGCTTGAATGGATTTCAACCTAAATTTCCAAAGGACTGGGGTGTAGTGGATTGGTCAAATGGCTGTGTGAGAAGGACTCCACTAAATTGCAGCGGTGACTTGTTTGAGAAGTACTCTGCAGTGAAATTGCCAAACAAAGAGCAGTCCTGGTTCAACAGAAGTATGAATCTCAAGGAATGTGAGATGGTGTGCCGGAAGAACTGTTCCTGCACAGCTTATGCAAATTTGGATATCAGGGATGGAGGATCTGGCTGCTTGCTGTGGTTTGGCAACCTAATTGATATTAGAAGTTTAACTGTAAATGGGCAAGATATTTATATAAGAATGGCTGCATCAGAACTAGTTAAGATTGTAGAATTTATAGGAATATTTGTTGTTATACCATCGCCTGGTAAGCCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

449

Amino Acids

50.2

Weight (kDa)

6.92

Isoelectric Point (pI)

37.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 77 - 181 2.9e-39 D-mannose binding lectin
S_locus_glycop PF00954 214 - 324 2.1e-28 S-locus glycoprotein domain
PAN_2 PF08276 345 - 410 2.4e-20 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 765
AccB7I CCANNNNNTGG 4 cut(s) 388, 659, 847, 1334
AccI GTMKAC 1 cut(s) 227
AciI CCGC 2 cut(s) 83, 1038
AclWI GGATC 3 cut(s) 551, 564, 1198
AcsI RAATTY 5 cut(s) 303, 612, 968, 1168, 1301
AcuI CTGAAG 2 cut(s) 505, 783
AfaI GTAC 3 cut(s) 100, 212, 1058
AfeI AGCGCT 1 cut(s) 818
AfiI CCNNNNNNNGG 6 cut(s) 179, 388, 566, 659, 847, 1334
AgsI TTSAA 8 cut(s) 16, 138, 619, 709, 783, 952, 962, 1099
AhlI ACTAGT 1 cut(s) 1285
AjnI CCWGG 4 cut(s) 298, 484, 1091, 1333
AloI GAACNNNNNNTCC 2 cut(s) 1132, 1164
AluBI AGCT 6 cut(s) 157, 385, 496, 760, 771, 1160
AluI AGCT 6 cut(s) 157, 385, 496, 760, 771, 1160
AlwI GGATC 3 cut(s) 551, 564, 1198
AlwNI CAGNNNCTG 1 cut(s) 434
Aor51HI AGCGCT 1 cut(s) 818
AoxI GGCC 2 cut(s) 288, 378
ApeKI GCWGC 6 cut(s) 90, 814, 945, 1035, 1197, 1274
ApoI RAATTY 5 cut(s) 303, 612, 968, 1168, 1301
AspLEI GCGC 1 cut(s) 819
AspS9I GGNCC 4 cut(s) 342, 379, 650, 902
AsuHPI GGTGA 1 cut(s) 1052
AvaII GGWCC 3 cut(s) 342, 650, 902
BamHI GGATCC 1 cut(s) 556
BbvI GCAGC 6 cut(s) 102, 826, 932, 1047, 1184, 1261
BccI CCATC 4 cut(s) 113, 1123, 1178, 1336
BceAI ACGGC 1 cut(s) 1327
BcgI CGANNNNNNTGC 2 cut(s) 872, 906
BciT130I CCWGG 4 cut(s) 300, 486, 1093, 1335
BclI TGATCA 1 cut(s) 730
BcuI ACTAGT 1 cut(s) 1285
BfaI CTAG 2 cut(s) 566, 1286
BfmI CTRYAG 4 cut(s) 129, 284, 363, 1062
BfoI RGCGCY 1 cut(s) 820
BglII AGATCT 1 cut(s) 359
BisI GCNGC 6 cut(s) 91, 815, 946, 1036, 1198, 1275
BlsI GCNGC 6 cut(s) 92, 816, 947, 1037, 1199, 1276
BmcAI AGTACT 1 cut(s) 1058
Bme1390I CCNGG 4 cut(s) 300, 486, 1093, 1335
Bme18I GGWCC 3 cut(s) 342, 650, 902
BmgT120I GGNCC 4 cut(s) 342, 379, 650, 902
BmiI GGNNCC 1 cut(s) 558
BmrFI CCNGG 4 cut(s) 300, 486, 1093, 1335
BmrI ACTGGG 2 cut(s) 929, 991
BmsI GCATC 2 cut(s) 538, 1286
BmuI ACTGGG 2 cut(s) 929, 991
BplI GAGNNNNNCTC 4 cut(s) 1006, 1038, 1043, 1075
BpuEI CTTGAG 1 cut(s) 1100
Bsa29I ATCGAT 1 cut(s) 193
BsaJI CCNNGG 2 cut(s) 298, 653
Bsc4I CCNNNNNNNGG 6 cut(s) 179, 388, 566, 659, 847, 1334
Bse1I ACTGG 6 cut(s) 148, 184, 374, 652, 935, 986
BseBI CCWGG 4 cut(s) 300, 486, 1093, 1335
BseCI ATCGAT 1 cut(s) 193
BseDI CCNNGG 2 cut(s) 298, 653
BseGI GGATG 3 cut(s) 30, 34, 1189
BseLI CCNNNNNNNGG 6 cut(s) 179, 388, 566, 659, 847, 1334
BseMII CTCAG 2 cut(s) 426, 786
BseNI ACTGG 6 cut(s) 148, 184, 374, 652, 935, 986
BseRI GAGGAG 1 cut(s) 305
BseXI GCAGC 6 cut(s) 102, 826, 932, 1047, 1184, 1261
BseYI CCCAGC 1 cut(s) 381
BsgI GTGCAG 2 cut(s) 833, 1138
BshFI GGCC 2 cut(s) 290, 380
BshVI ATCGAT 1 cut(s) 193
BsiSI CCGG 1 cut(s) 1138
BslI CCNNNNNNNGG 6 cut(s) 179, 388, 566, 659, 847, 1334
BsmI GAATGC 1 cut(s) 893
BsnI GGCC 2 cut(s) 290, 380
Bsp143I GATC 5 cut(s) 359, 556, 589, 730, 1190
Bsp19I CCATGG 1 cut(s) 653
BspACI CCGC 2 cut(s) 83, 1038
BspANI GGCC 2 cut(s) 290, 380
BspCNI CTCAG 2 cut(s) 427, 785
BspDI ATCGAT 1 cut(s) 193
BspHI TCATGA 1 cut(s) 733
BspLI GGNNCC 1 cut(s) 558
BspMAI CTGCAG 3 cut(s) 288, 367, 1066
BspPI GGATC 3 cut(s) 551, 564, 1198
BsrI ACTGG 6 cut(s) 148, 184, 374, 652, 935, 986
BssECI CCNNGG 2 cut(s) 298, 653
BssMI GATC 5 cut(s) 359, 556, 589, 730, 1190
BssT1I CCWWGG 1 cut(s) 653
Bst2UI CCWGG 4 cut(s) 300, 486, 1093, 1335
Bst4CI ACNGT 4 cut(s) 868, 929, 1148, 1243
BstC8I GCNNGC 4 cut(s) 288, 680, 916, 1202
BstDEI CTNAG 2 cut(s) 435, 772
BstDSI CCRYGG 1 cut(s) 653
BstENI CCTNNNNNAGG 1 cut(s) 564
BstF5I GGATG 3 cut(s) 30, 34, 1189
BstH2I RGCGCY 1 cut(s) 820
BstHHI GCGC 1 cut(s) 819
BstKTI GATC 5 cut(s) 362, 559, 592, 733, 1193
BstMBI GATC 5 cut(s) 359, 556, 589, 730, 1190
BstMWI GCNNNNNNNGC 2 cut(s) 814, 1339
BstNI CCWGG 4 cut(s) 300, 486, 1093, 1335
BstNSI RCATGY 1 cut(s) 918
BstSCI CCNGG 4 cut(s) 298, 484, 1091, 1333
BstSFI CTRYAG 4 cut(s) 129, 284, 363, 1062
BstV1I GCAGC 6 cut(s) 102, 826, 932, 1047, 1184, 1261
BstX2I RGATCY 3 cut(s) 359, 556, 1190
BstXI CCANNNNNNTGG 1 cut(s) 981
BstYI RGATCY 3 cut(s) 359, 556, 1190
Bsu15I ATCGAT 1 cut(s) 193
BsuRI GGCC 2 cut(s) 290, 380
BsuTUI ATCGAT 1 cut(s) 193
BtgI CCRYGG 1 cut(s) 653
BtgZI GCGATG 1 cut(s) 1314
BtsCI GGATG 3 cut(s) 30, 34, 1189
BtsI GCAGTG 2 cut(s) 427, 1071
BtsIMutI CAGTG 3 cut(s) 381, 427, 1071
Cac8I GCNNGC 4 cut(s) 288, 680, 916, 1202
CaiI CAGNNNCTG 1 cut(s) 434
CciI TCATGA 1 cut(s) 733
CfoI GCGC 1 cut(s) 819
Cfr13I GGNCC 4 cut(s) 342, 379, 650, 902
ClaI ATCGAT 1 cut(s) 193
CsiI ACCWGGT 1 cut(s) 484
Csp6I GTAC 3 cut(s) 99, 211, 1057
CviAII CATG 5 cut(s) 540, 654, 734, 825, 915
CviQI GTAC 3 cut(s) 99, 211, 1057
DdeI CTNAG 2 cut(s) 435, 772
DpnI GATC 5 cut(s) 361, 558, 591, 732, 1192
DpnII GATC 5 cut(s) 359, 556, 589, 730, 1190
Eco130I CCWWGG 1 cut(s) 653
Eco32I GATATC 2 cut(s) 222, 1177
Eco47I GGWCC 3 cut(s) 342, 650, 902
Eco47III AGCGCT 1 cut(s) 818
Eco57I CTGAAG 2 cut(s) 505, 783
EcoNI CCTNNNNNAGG 1 cut(s) 564
EcoRI GAATTC 1 cut(s) 303
EcoRII CCWGG 4 cut(s) 298, 484, 1091, 1333
EcoRV GATATC 2 cut(s) 222, 1177
EcoT14I CCWWGG 1 cut(s) 653
EcoT22I ATGCAT 1 cut(s) 895
ErhI CCWWGG 1 cut(s) 653
FaeI CATG 5 cut(s) 543, 657, 737, 828, 918
FalI AAGNNNNNCTT 2 cut(s) 246, 278
FatI CATG 5 cut(s) 539, 653, 733, 824, 914
FauI CCCGC 1 cut(s) 76
FauNDI CATATG 1 cut(s) 580
FbaI TGATCA 1 cut(s) 730
FblI GTMKAC 1 cut(s) 227
Fnu4HI GCNGC 6 cut(s) 91, 815, 946, 1036, 1198, 1275
FokI GGATG 3 cut(s) 21, 37, 1196
Fsp4HI GCNGC 6 cut(s) 91, 815, 946, 1036, 1198, 1275
FspBI CTAG 2 cut(s) 566, 1286
GlaI GCGC 1 cut(s) 818
GluI GCNGC 6 cut(s) 91, 815, 946, 1036, 1198, 1275
GsaI CCCAGC 1 cut(s) 385
HaeII RGCGCY 1 cut(s) 820
HaeIII GGCC 2 cut(s) 290, 380
HapII CCGG 1 cut(s) 1138
HhaI GCGC 1 cut(s) 819
Hin1II CATG 5 cut(s) 543, 657, 737, 828, 918
Hin6I GCGC 1 cut(s) 817
HinP1I GCGC 1 cut(s) 817
HindIII AAGCTT 1 cut(s) 494
HinfI GANTC 8 cut(s) 269, 370, 392, 626, 663, 852, 1021, 1111
HpaII CCGG 1 cut(s) 1138
HphI GGTGA 1 cut(s) 1052
Hpy166II GTNNAC 2 cut(s) 228, 551
Hpy188I TCNGA 4 cut(s) 126, 668, 802, 1282
Hpy188III TCNNGA 4 cut(s) 357, 396, 734, 783
Hpy8I GTNNAC 2 cut(s) 228, 551
HpyAV CCTTC 6 cut(s) 10, 183, 570, 616, 797, 1011
HpyCH4III ACNGT 4 cut(s) 868, 929, 1148, 1243
HpyF10VI GCNNNNNNNGC 2 cut(s) 814, 1339
HpyF3I CTNAG 2 cut(s) 435, 772
Hsp92II CATG 5 cut(s) 543, 657, 737, 828, 918
HspAI GCGC 1 cut(s) 817
Ksp22I TGATCA 1 cut(s) 730
Kzo9I GATC 5 cut(s) 359, 556, 589, 730, 1190
Lsp1109I GCAGC 6 cut(s) 102, 826, 932, 1047, 1184, 1261
LweI GCATC 2 cut(s) 538, 1286
MabI ACCWGGT 1 cut(s) 484
MaeI CTAG 2 cut(s) 566, 1286
MaeIII GTNAC 4 cut(s) 409, 511, 793, 1040
MalI GATC 5 cut(s) 361, 558, 591, 732, 1192
MboI GATC 5 cut(s) 359, 556, 589, 730, 1190
MboII GAAGA 5 cut(s) 229, 620, 631, 752, 1153
MflI RGATCY 3 cut(s) 359, 556, 1190
MluCI AATT 9 cut(s) 303, 612, 715, 968, 1030, 1070, 1168, 1221, 1301
MlyI GAGTC 2 cut(s) 635, 1015
MmeI TCCRAC 1 cut(s) 482
MnlI CCTC 7 cut(s) 80, 323, 326, 355, 590, 693, 1181
Mph1103I ATGCAT 1 cut(s) 895
MseI TTAA 3 cut(s) 689, 1238, 1290
MspA1I CMGCKG 2 cut(s) 83, 1038
MspI CCGG 1 cut(s) 1138
MspR9I CCNGG 4 cut(s) 300, 486, 1093, 1335
Mva1269I GAATGC 1 cut(s) 893
MvaI CCWGG 4 cut(s) 300, 486, 1093, 1335
MwoI GCNNNNNNNGC 2 cut(s) 814, 1339
NcoI CCATGG 1 cut(s) 653
NdeI CATATG 1 cut(s) 580
NdeII GATC 5 cut(s) 359, 556, 589, 730, 1190
NlaIII CATG 5 cut(s) 543, 657, 737, 828, 918
NlaIV GGNNCC 1 cut(s) 558
NmuCI GTSAC 2 cut(s) 793, 1040
NsiI ATGCAT 1 cut(s) 895
NspI RCATGY 1 cut(s) 918
PaeI GCATGC 1 cut(s) 918
PagI TCATGA 1 cut(s) 733
PctI GAATGC 1 cut(s) 893
PfeI GAWTC 6 cut(s) 269, 370, 392, 663, 852, 1111
PflMI CCANNNNNTGG 4 cut(s) 388, 659, 847, 1334
PkrI GCNGC 6 cut(s) 92, 816, 947, 1037, 1199, 1276
PleI GAGTC 2 cut(s) 634, 1015
PpsI GAGTC 2 cut(s) 634, 1015
PsiI TTATAA 1 cut(s) 765
Psp6I CCWGG 4 cut(s) 298, 484, 1091, 1333
PspFI CCCAGC 1 cut(s) 381
PspGI CCWGG 4 cut(s) 298, 484, 1091, 1333
PspN4I GGNNCC 1 cut(s) 558
PspPI GGNCC 4 cut(s) 342, 379, 650, 902
PstI CTGCAG 3 cut(s) 288, 367, 1066
PstNI CAGNNNCTG 1 cut(s) 434
PsuI RGATCY 3 cut(s) 359, 556, 1190
RsaI GTAC 3 cut(s) 100, 212, 1058
RsaNI GTAC 3 cut(s) 99, 211, 1057
SaqAI TTAA 3 cut(s) 689, 1238, 1290
SatI GCNGC 6 cut(s) 91, 815, 946, 1036, 1198, 1275
Sau3AI GATC 5 cut(s) 359, 556, 589, 730, 1190
Sau96I GGNCC 4 cut(s) 342, 379, 650, 902
ScaI AGTACT 1 cut(s) 1058
SchI GAGTC 2 cut(s) 635, 1015
ScrFI CCNGG 4 cut(s) 300, 486, 1093, 1335
SexAI ACCWGGT 1 cut(s) 484
SfaNI GCATC 2 cut(s) 538, 1286
SfcI CTRYAG 4 cut(s) 129, 284, 363, 1062
SinI GGWCC 3 cut(s) 342, 650, 902
SmlI CTYRAG 1 cut(s) 1115
SmoI CTYRAG 1 cut(s) 1115
SpeI ACTAGT 1 cut(s) 1285
SphI GCATGC 1 cut(s) 918
Sse9I AATT 9 cut(s) 303, 612, 715, 968, 1030, 1070, 1168, 1221, 1301
SsiI CCGC 2 cut(s) 83, 1038
SspI AATATT 3 cut(s) 746, 922, 1314
SspMI CTAG 2 cut(s) 566, 1286
StyD4I CCNGG 4 cut(s) 298, 484, 1091, 1333
StyI CCWWGG 1 cut(s) 653
TaaI ACNGT 4 cut(s) 868, 929, 1148, 1243
TaqI TCGA 1 cut(s) 193
TasI AATT 9 cut(s) 303, 612, 715, 968, 1030, 1070, 1168, 1221, 1301
TatI WGTACW 2 cut(s) 98, 1056
TfiI GAWTC 6 cut(s) 269, 370, 392, 663, 852, 1111
Tru1I TTAA 3 cut(s) 689, 1238, 1290
Tru9I TTAA 3 cut(s) 689, 1238, 1290
TscAI CASTG 3 cut(s) 381, 427, 1071
TseFI GTSAC 2 cut(s) 793, 1040
TseI GCWGC 6 cut(s) 90, 814, 945, 1035, 1197, 1274
Tsp45I GTSAC 2 cut(s) 793, 1040
TspDTI ATGAA 6 cut(s) 17, 261, 506, 597, 753, 1124
TspGWI ACGGA 1 cut(s) 691
TspRI CASTG 3 cut(s) 381, 427, 1071
Van91I CCANNNNNTGG 4 cut(s) 388, 659, 847, 1334
VpaK11BI GGWCC 3 cut(s) 342, 650, 902
XagI CCTNNNNNAGG 1 cut(s) 564
XapI RAATTY 5 cut(s) 303, 612, 968, 1168, 1301
XceI RCATGY 1 cut(s) 918
XmiI GTMKAC 1 cut(s) 227
XspI CTAG 2 cut(s) 566, 1286
ZrmI AGTACT 1 cut(s) 1058
Zsp2I ATGCAT 1 cut(s) 895
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.