RchiOBHm_Chr5g0026381

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000484
Physical Location & Seq
Forward (+)
20422752 .. 20425909
3158 bp
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UTR
Exon/CDS
Intron
PRQ30596

Sequence Viewer

Length: 2328 bp
ATGATGTGCGGCTTGCAACTCTTTTGCTGTTCTTTACTGCTCATCATTGCAGCAACGCTTGCCACAGCAGAAGCCAGCACAAGTACATTTCAGTCCATCAAAGACGGCGAGACCATAGTTTCAACCGATGGAACCTTTGAGCTCGGATTCTTCACTCCTGCAGGCGCTTCTGGTAATCGATACATAGGCATATGGTACAAGAAGATATCGGTTACAACGGTTGTGTGGGTTGCCAACAGAGAAACACCCCTCACTGATTCATCAGGTATTCTCAGAATCACTGACCCTGGAATTCTTGTCCTTGTCAACCAGAACAACACCACAGTTTGGTCCTCCAACACATCAACAACCGCACCAAATGCAGTGGCACAGCTTTTGGATTCAGGAAACCTTGTTGTGAAAGATGGCAGTGACAAAGTTGATGCAGAGGATGAGAAGTACTTGTGGCAGAGTTTTGACTACCCGGGTGATACATTGCTAGCAGGTATGAAGCTTGGTAGGAACACAGTTACAGGCTTCAATTGGCATCTTACATCATGGAGAAGTCCCTCTGATCCTTCTCAAGGCAACTTCACATTTCAACATGGTCCCAGGGGATATGCAGAACAAGTTCTGAGGGAGGGTTCAGACATAACATTTCGGACTGGACCATGGAATGGAATCCGGTTCAGTGGAACGCCTCATTTAGGTCCAAACTATGTTTACACATATCAGCTAGTTTTTGATGATCATGATCATGAAGAATACTATAGCTATAAGCTTCTCAACAGCTCAGTTCTTTCCAGGCTGGTGTTAACTCAAGATGGGCTTCTGCAGCGCTACACATGGATCGATAGAACCCAAAGTTGGTTTCTTTACTTAACAGCACAGATGGATAACTGTGACAATTATGCACTTTGTGGTGCATATGGTGCTTGCAATATTGAGAACTCCCCAGTATGCGGCTGCTTGAAAGGTTTTGTGCCAAAATTTCCAAAAGATTGGGACACAATGGATTGGTCAAATGGGTGTGTGAGAAAGACTCCACTAGCTTGCAATGGCGACAAGTTTCTGAAGTACTCTGGGGTCAAATTTCCAAACACAGAACAATCCTGGTTTAACAAAAGTATGAACCTCAAGGAATGCGAGATGGAGTGCACCAGAAACTGTTCCTGCACAGCTTATTCAAATTTGTATATTAGCGAAGGAGGAACTGGGTGCTTGATGTGGTTTGGCGATCTGATTGATATGAGAAATCTCACTGAAAATGGACAAGATATTTACATAAGGATGGCAGCATCAGAACAAGGAAAACTGACGCACAGCAAGAAGGAAGATCTTGAATTACCATTGTTTGACTTGGCTACTATTGTCTCTGCAACCAGCAACTTTTCAAACGACAATAAGCTTGGAGAAGGTGGTTTTGGATGCGTCTTTAAGGGTATGTTGAGAAATGGGCAAGAAATAGCTGTCAAAAGGCTCTCAAAACATTCTTCACAAGGAGTAAATGAGTTCAAAAATGAGGTTACGCATATTGCCAAACTCCAGCACAGGAATCTAGTGAAGCTCCTTGGATGTTGCATTCAAGCCGATGAAATGATGCTCATCTACGAATGCATGCCTAACAAAAGCTTGGACTTCTTTATTTTTGATCAAAGAAAAAGCATGCTACTAGATTGGCCTAAGCGCTTTGACATCATCAATGGTATTGCTCGGGGGCTCCTCTATCTTCATCAAGATTCTAGACTGAGAGTAATTCATAGAGATCTCAAAGCAGGCAATATTTTATTAGACAATGAATTTAACCCCAAGATCTCAGACTTTGGTCTAGCTAGAAGTTTTGGAGGAAATGAAACTGTAGCAGAAACGATGAGAGTGGTTGGAACCTATGGTTACATGTCCCCAGAATATGCAAGTGACGGGTTCTACTCTACCAAATCTGATGTTTTTAGCTTTGGTGTTTCGGTGTTGGAGATAATAAGTGGGAAGAGAAACAGAGGATTCTCTCATCCAGACCACGACCTCAACCTTCTTGGACATGCATGGAATCTATACACAGAAGGCAGGTCCATAGAATTGCTTGATACATCAGTAGGGGACTCCACTAATCCATCCGAGGAAGTTCTGCGCTCGATTCATGTGGGTCTTTTATGTGTGCAGAAAGATCCAGAAGATAGGCCAAGCATGTCAGATGTTGTTCGAATGTTGAGTGGTGAAGGTGCATTGTCTCAACCTGAAAAACCTGGGTTTTATAGTAAAAGTAGTGATAGGTCTGAAAACCAAGTCAATCATTTATCTAGAGCATGCTCGGCTAATGAAGTTAGTTTTACGCTACTTGATGCTAGATGA

Protein Analysis

775

Amino Acids

86.56

Weight (kDa)

5.5

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 73 - 181 3.6e-38 D-mannose binding lectin
S_locus_glycop PF00954 213 - 324 3e-33 S-locus glycoprotein domain
PAN_2 PF08276 345 - 410 1.5e-20 PAN-like domain
Pkinase PF00069 458 - 727 2.4e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 460 - 729 1.1e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 473, 2034
AccB7I CCANNNNNTGG 2 cut(s) 327, 656
AciI CCGC 3 cut(s) 9, 351, 942
AclWI GGATC 3 cut(s) 548, 836, 2138
AcsI RAATTY 5 cut(s) 291, 968, 1070, 1168, 1778
AcuI CTGAAG 1 cut(s) 1073
AdeI CACNNNGTG 1 cut(s) 899
AfaI GTAC 4 cut(s) 85, 197, 440, 1058
AfeI AGCGCT 2 cut(s) 818, 1667
AfiI CCNNNNNNNGG 6 cut(s) 327, 563, 656, 686, 846, 941
AflIII ACRYGT 1 cut(s) 1875
AgsI TTSAA 9 cut(s) 123, 520, 581, 952, 1167, 1322, 1374, 1495, 1565
AjnI CCWGG 5 cut(s) 286, 590, 782, 1091, 2221
AjuI GAANNNNNNNTTGG 6 cut(s) 829, 834, 861, 866, 1386, 1418
Alw21I GWGCWC 2 cut(s) 144, 1139
Alw26I GTCTC 3 cut(s) 104, 1357, 2211
Alw44I GTGCAC 1 cut(s) 1135
AlwI GGATC 3 cut(s) 548, 836, 2138
AlwNI CAGNNNCTG 1 cut(s) 1146
Ama87I CYCGRG 2 cut(s) 463, 1692
Aor51HI AGCGCT 2 cut(s) 818, 1667
AoxI GGCC 2 cut(s) 1658, 2156
ApaLI GTGCAC 1 cut(s) 1135
ApeKI GCWGC 4 cut(s) 50, 814, 945, 1274
ApoI RAATTY 5 cut(s) 291, 968, 1070, 1168, 1778
Asp700I GAANNNNTTC 2 cut(s) 609, 1147
AspLEI GCGC 4 cut(s) 167, 819, 1668, 2109
AspS9I GGNCC 5 cut(s) 330, 587, 647, 689, 2046
AsuC2I CCSGG 2 cut(s) 464, 465
AsuHPI GGTGA 2 cut(s) 479, 2204
AsuII TTCGAA 1 cut(s) 2179
AsuNHI GCTAGC 1 cut(s) 478
AvaI CYCGRG 2 cut(s) 463, 1692
AvaII GGWCC 5 cut(s) 330, 587, 647, 689, 2046
BaeGI GKGCMC 1 cut(s) 1139
BaeI ACNNNNGTAYC 2 cut(s) 2055, 2088
BanII GRGCYC 2 cut(s) 144, 1701
Bbv12I GWGCWC 2 cut(s) 144, 1139
BbvI GCAGC 4 cut(s) 62, 826, 932, 1286
BccI CCATC 8 cut(s) 104, 122, 398, 797, 865, 1123, 1264, 2098
BceAI ACGGC 1 cut(s) 121
BciT130I CCWGG 5 cut(s) 288, 592, 784, 1093, 2223
BclI TGATCA 3 cut(s) 727, 733, 1630
BcnI CCSGG 2 cut(s) 464, 465
BcoDI GTCTC 3 cut(s) 104, 1357, 2211
BfmI CTRYAG 4 cut(s) 159, 748, 812, 1836
BfoI RGCGCY 3 cut(s) 168, 820, 1669
BfuAI ACCTGC 2 cut(s) 473, 2034
BglII AGATCT 3 cut(s) 1315, 1744, 1791
BisI GCNGC 6 cut(s) 10, 51, 815, 943, 946, 1275
BlsI GCNGC 6 cut(s) 11, 52, 816, 944, 947, 1276
BmcAI AGTACT 2 cut(s) 440, 1058
Bme1390I CCNGG 7 cut(s) 288, 464, 465, 592, 784, 1093, 2223
Bme18I GGWCC 5 cut(s) 330, 587, 647, 689, 2046
BmeT110I CYCGRG 2 cut(s) 463, 1692
BmgT120I GGNCC 5 cut(s) 330, 587, 647, 689, 2046
BmiI GGNNCC 4 cut(s) 133, 589, 1700, 1864
BmrFI CCNGG 7 cut(s) 288, 464, 465, 592, 784, 1093, 2223
BmrI ACTGGG 2 cut(s) 929, 1203
BmsI GCATC 6 cut(s) 412, 535, 1286, 1397, 1569, 2308
BmtI GCTAGC 1 cut(s) 482
BmuI ACTGGG 2 cut(s) 929, 1203
BoxI GACNNNNGTC 1 cut(s) 1803
BplI GAGNNNNNCTC 2 cut(s) 1006, 1038
BpmI CTGGAG 1 cut(s) 1508
Bpu10I CCTNAGC 1 cut(s) 1662
Bpu14I TTCGAA 1 cut(s) 2179
BpuEI CTTGAG 3 cut(s) 546, 783, 1100
BpuMI CCSGG 2 cut(s) 464, 465
Bsa29I ATCGAT 2 cut(s) 178, 831
BsaBI GATNNNNATC 2 cut(s) 732, 1583
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 8 cut(s) 286, 463, 590, 591, 650, 1549, 2094, 2222
BsaWI WCCGGW 1 cut(s) 663
Bsc4I CCNNNNNNNGG 6 cut(s) 327, 563, 656, 686, 846, 941
Bse1I ACTGG 3 cut(s) 649, 935, 1198
Bse3DI GCAATG 3 cut(s) 45, 473, 1042
Bse8I GATNNNNATC 2 cut(s) 732, 1583
BseBI CCWGG 5 cut(s) 288, 592, 784, 1093, 2223
BseCI ATCGAT 2 cut(s) 178, 831
BseDI CCNNGG 8 cut(s) 286, 463, 590, 591, 650, 1549, 2094, 2222
BseGI GGATG 6 cut(s) 436, 1275, 1412, 1559, 1987, 2090
BseJI GATNNNNATC 2 cut(s) 732, 1583
BseLI CCNNNNNNNGG 6 cut(s) 327, 563, 656, 686, 846, 941
BseMI GCAATG 3 cut(s) 45, 473, 1042
BseMII CTCAG 5 cut(s) 286, 605, 786, 1718, 1809
BseNI ACTGG 3 cut(s) 649, 935, 1198
BseRI GAGGAG 1 cut(s) 1691
BseSI GKGCMC 1 cut(s) 1139
BseXI GCAGC 4 cut(s) 62, 826, 932, 1286
BsgI GTGCAG 2 cut(s) 1138, 2156
BshFI GGCC 2 cut(s) 1660, 2158
BshVI ATCGAT 2 cut(s) 178, 831
BsiHKAI GWGCWC 2 cut(s) 144, 1139
BsiHKCI CYCGRG 2 cut(s) 463, 1692
BsiSI CCGG 2 cut(s) 464, 664
BslFI GGGAC 5 cut(s) 531, 573, 998, 1864, 2090
BslI CCNNNNNNNGG 6 cut(s) 327, 563, 656, 686, 846, 941
BsmAI GTCTC 3 cut(s) 104, 1357, 2211
BsmFI GGGAC 5 cut(s) 531, 573, 998, 1864, 2090
BsmI GAATGC 3 cut(s) 1127, 1560, 1598
BsnI GGCC 2 cut(s) 1660, 2158
Bso31I GGTCTC 1 cut(s) 104
BsoBI CYCGRG 2 cut(s) 463, 1692
Bsp119I TTCGAA 1 cut(s) 2179
Bsp1286I GDGCHC 3 cut(s) 144, 1139, 1701
Bsp19I CCATGG 1 cut(s) 650
BspACI CCGC 3 cut(s) 9, 351, 942
BspANI GGCC 2 cut(s) 1660, 2158
BspCNI CTCAG 5 cut(s) 285, 606, 785, 1719, 1808
BspDI ATCGAT 2 cut(s) 178, 831
BspHI TCATGA 2 cut(s) 730, 736
BspLI GGNNCC 4 cut(s) 133, 589, 1700, 1864
BspMAI CTGCAG 2 cut(s) 163, 816
BspMI ACCTGC 2 cut(s) 473, 2034
BspOI GCTAGC 1 cut(s) 482
BspPI GGATC 3 cut(s) 548, 836, 2138
BspT104I TTCGAA 1 cut(s) 2179
BspTNI GGTCTC 1 cut(s) 104
BsrDI GCAATG 3 cut(s) 45, 473, 1042
BsrI ACTGG 3 cut(s) 649, 935, 1198
BssECI CCNNGG 8 cut(s) 286, 463, 590, 591, 650, 1549, 2094, 2222
BssT1I CCWWGG 2 cut(s) 650, 1549
Bst2UI CCWGG 5 cut(s) 288, 592, 784, 1093, 2223
Bst4CI ACNGT 6 cut(s) 220, 325, 508, 881, 1148, 1837
Bst6I CTCTTC 1 cut(s) 1961
BstAPI GCANNNNNTGC 3 cut(s) 59, 359, 911
BstBI TTCGAA 1 cut(s) 2179
BstDEI CTNAG 6 cut(s) 272, 614, 772, 1662, 1727, 1795
BstDSI CCRYGG 1 cut(s) 650
BstENI CCTNNNNNAGG 2 cut(s) 561, 684
BstF5I GGATG 6 cut(s) 436, 1275, 1412, 1559, 1987, 2090
BstH2I RGCGCY 3 cut(s) 168, 820, 1669
BstHHI GCGC 4 cut(s) 167, 819, 1668, 2109
BstMAI GTCTC 3 cut(s) 104, 1357, 2211
BstMWI GCNNNNNNNGC 5 cut(s) 59, 359, 814, 911, 2288
BstNI CCWGG 5 cut(s) 288, 592, 784, 1093, 2223
BstNSI RCATGY 6 cut(s) 1600, 1648, 1879, 2021, 2167, 2286
BstPAI GACNNNNGTC 1 cut(s) 1803
BstSCI CCNGG 7 cut(s) 286, 462, 463, 590, 782, 1091, 2221
BstSFI CTRYAG 4 cut(s) 159, 748, 812, 1836
BstSLI GKGCMC 1 cut(s) 1139
BstV1I GCAGC 4 cut(s) 62, 826, 932, 1286
BstX2I RGATCY 4 cut(s) 1315, 1744, 1791, 2143
BstXI CCANNNNNNTGG 1 cut(s) 981
BstYI RGATCY 4 cut(s) 1315, 1744, 1791, 2143
Bsu15I ATCGAT 2 cut(s) 178, 831
BsuRI GGCC 2 cut(s) 1660, 2158
BsuTUI ATCGAT 2 cut(s) 178, 831
BtgI CCRYGG 1 cut(s) 650
BtsCI GGATG 6 cut(s) 436, 1275, 1412, 1559, 1987, 2090
BtsI GCAGTG 2 cut(s) 369, 415
BtsIMutI CAGTG 6 cut(s) 252, 279, 369, 415, 676, 1239
BveI ACCTGC 2 cut(s) 473, 2034
CaiI CAGNNNCTG 1 cut(s) 1146
CciI TCATGA 2 cut(s) 730, 736
CfoI GCGC 4 cut(s) 167, 819, 1668, 2109
Cfr13I GGNCC 5 cut(s) 330, 587, 647, 689, 2046
Cfr9I CCCGGG 1 cut(s) 463
ClaI ATCGAT 2 cut(s) 178, 831
CseI GACGC 2 cut(s) 1306, 1399
Csp6I GTAC 4 cut(s) 84, 196, 439, 1057
CspCI CAANNNNNGTGG 2 cut(s) 345, 380
CviQI GTAC 4 cut(s) 84, 196, 439, 1057
DdeI CTNAG 6 cut(s) 272, 614, 772, 1662, 1727, 1795
DraIII CACNNNGTG 1 cut(s) 899
Eam1104I CTCTTC 1 cut(s) 1961
EarI CTCTTC 1 cut(s) 1961
Ecl136II GAGCTC 1 cut(s) 142
Eco130I CCWWGG 2 cut(s) 650, 1549
Eco24I GRGCYC 2 cut(s) 144, 1701
Eco31I GGTCTC 1 cut(s) 104
Eco32I GATATC 1 cut(s) 207
Eco47I GGWCC 5 cut(s) 330, 587, 647, 689, 2046
Eco47III AGCGCT 2 cut(s) 818, 1667
Eco53kI GAGCTC 1 cut(s) 142
Eco57I CTGAAG 1 cut(s) 1073
Eco88I CYCGRG 2 cut(s) 463, 1692
EcoICRI GAGCTC 1 cut(s) 142
EcoNI CCTNNNNNAGG 2 cut(s) 561, 684
EcoRI GAATTC 1 cut(s) 291
EcoRII CCWGG 5 cut(s) 286, 590, 782, 1091, 2221
EcoRV GATATC 1 cut(s) 207
EcoT14I CCWWGG 2 cut(s) 650, 1549
EcoT22I ATGCAT 2 cut(s) 1598, 2023
EcoT38I GRGCYC 2 cut(s) 144, 1701
ErhI CCWWGG 2 cut(s) 650, 1549
FalI AAGNNNNNCTT 2 cut(s) 792, 824
FaqI GGGAC 5 cut(s) 531, 573, 998, 1864, 2090
FauNDI CATATG 2 cut(s) 191, 907
FbaI TGATCA 3 cut(s) 727, 733, 1630
Fnu4HI GCNGC 6 cut(s) 10, 51, 815, 943, 946, 1275
FokI GGATG 6 cut(s) 443, 1282, 1419, 1566, 1974, 2077
FriOI GRGCYC 2 cut(s) 144, 1701
Fsp4HI GCNGC 6 cut(s) 10, 51, 815, 943, 946, 1275
GlaI GCGC 4 cut(s) 166, 818, 1667, 2108
GluI GCNGC 6 cut(s) 10, 51, 815, 943, 946, 1275
GsuI CTGGAG 1 cut(s) 1508
HaeII RGCGCY 3 cut(s) 168, 820, 1669
HaeIII GGCC 2 cut(s) 1660, 2158
HapII CCGG 2 cut(s) 464, 664
HgaI GACGC 2 cut(s) 1306, 1399
HhaI GCGC 4 cut(s) 167, 819, 1668, 2109
Hin6I GCGC 4 cut(s) 165, 817, 1666, 2107
HinP1I GCGC 4 cut(s) 165, 817, 1666, 2107
HincII GTYRAC 2 cut(s) 307, 795
HindII GTYRAC 2 cut(s) 307, 795
HindIII AAGCTT 4 cut(s) 491, 758, 1385, 1609
HpaI GTTAAC 1 cut(s) 795
HpaII CCGG 2 cut(s) 464, 664
HphI GGTGA 2 cut(s) 479, 2204
Hpy166II GTNNAC 4 cut(s) 307, 703, 795, 1137
Hpy8I GTNNAC 4 cut(s) 307, 703, 795, 1137
HpyAV CCTTC 7 cut(s) 567, 1178, 1303, 1388, 2018, 2033, 2189
HpyCH4III ACNGT 6 cut(s) 220, 325, 508, 881, 1148, 1837
HpyF10VI GCNNNNNNNGC 5 cut(s) 59, 359, 814, 911, 2288
HpyF3I CTNAG 6 cut(s) 272, 614, 772, 1662, 1727, 1795
HspAI GCGC 4 cut(s) 165, 817, 1666, 2107
Ksp22I TGATCA 3 cut(s) 727, 733, 1630
KspAI GTTAAC 1 cut(s) 795
LmnI GCTCC 2 cut(s) 1551, 1704
Lsp1109I GCAGC 4 cut(s) 62, 826, 932, 1286
LweI GCATC 6 cut(s) 412, 535, 1286, 1397, 1569, 2308
MaeIII GTNAC 7 cut(s) 211, 410, 508, 881, 1504, 1871, 1895
MboII GAAGA 8 cut(s) 142, 214, 752, 1325, 1464, 1700, 1978, 2162
MfeI CAATTG 1 cut(s) 520
MflI RGATCY 4 cut(s) 1315, 1744, 1791, 2143
MhlI GDGCHC 3 cut(s) 144, 1139, 1701
MlyI GAGTC 2 cut(s) 1015, 2072
MmeI TCCRAC 3 cut(s) 360, 1840, 1929
Mph1103I ATGCAT 2 cut(s) 1598, 2023
MroXI GAANNNNTTC 2 cut(s) 609, 1147
MseI TTAA 5 cut(s) 794, 860, 1098, 1416, 1782
MslI CAYNNNNRTG 2 cut(s) 735, 1268
MspI CCGG 2 cut(s) 464, 664
MspR9I CCNGG 7 cut(s) 288, 464, 465, 592, 784, 1093, 2223
MunI CAATTG 1 cut(s) 520
Mva1269I GAATGC 3 cut(s) 1127, 1560, 1598
MvaI CCWGG 5 cut(s) 288, 592, 784, 1093, 2223
MwoI GCNNNNNNNGC 5 cut(s) 59, 359, 814, 911, 2288
NciI CCSGG 2 cut(s) 464, 465
NcoI CCATGG 1 cut(s) 650
NdeI CATATG 2 cut(s) 191, 907
NheI GCTAGC 1 cut(s) 478
NlaIV GGNNCC 4 cut(s) 133, 589, 1700, 1864
NmeAIII GCCGAG 1 cut(s) 2267
NmuCI GTSAC 3 cut(s) 410, 881, 1895
NsiI ATGCAT 2 cut(s) 1598, 2023
NspI RCATGY 6 cut(s) 1600, 1648, 1879, 2021, 2167, 2286
NspV TTCGAA 1 cut(s) 2179
PaeI GCATGC 3 cut(s) 1600, 1648, 2286
PagI TCATGA 2 cut(s) 730, 736
PasI CCCWGGG 1 cut(s) 591
PciI ACATGT 1 cut(s) 1875
PctI GAATGC 3 cut(s) 1127, 1560, 1598
PdmI GAANNNNTTC 2 cut(s) 609, 1147
PflMI CCANNNNNTGG 2 cut(s) 327, 656
PkrI GCNGC 6 cut(s) 11, 52, 816, 944, 947, 1276
PleI GAGTC 2 cut(s) 1015, 2072
PpsI GAGTC 2 cut(s) 1015, 2072
PscI ACATGT 1 cut(s) 1875
PshAI GACNNNNGTC 1 cut(s) 1803
Psp124BI GAGCTC 1 cut(s) 144
Psp6I CCWGG 5 cut(s) 286, 590, 782, 1091, 2221
PspGI CCWGG 5 cut(s) 286, 590, 782, 1091, 2221
PspN4I GGNNCC 4 cut(s) 133, 589, 1700, 1864
PspPI GGNCC 5 cut(s) 330, 587, 647, 689, 2046
PstI CTGCAG 2 cut(s) 163, 816
PstNI CAGNNNCTG 1 cut(s) 1146
PsuI RGATCY 4 cut(s) 1315, 1744, 1791, 2143
RsaI GTAC 4 cut(s) 85, 197, 440, 1058
RsaNI GTAC 4 cut(s) 84, 196, 439, 1057
RseI CAYNNNNRTG 2 cut(s) 735, 1268
SacI GAGCTC 1 cut(s) 144
SaqAI TTAA 5 cut(s) 794, 860, 1098, 1416, 1782
SatI GCNGC 6 cut(s) 10, 51, 815, 943, 946, 1275
Sau96I GGNCC 5 cut(s) 330, 587, 647, 689, 2046
SbfI CCTGCAGG 1 cut(s) 163
ScaI AGTACT 2 cut(s) 440, 1058
SchI GAGTC 2 cut(s) 1015, 2072
ScrFI CCNGG 7 cut(s) 288, 464, 465, 592, 784, 1093, 2223
SdaI CCTGCAGG 1 cut(s) 163
SduI GDGCHC 3 cut(s) 144, 1139, 1701
SfaNI GCATC 6 cut(s) 412, 535, 1286, 1397, 1569, 2308
SfcI CTRYAG 4 cut(s) 159, 748, 812, 1836
SfuI TTCGAA 1 cut(s) 2179
SinI GGWCC 5 cut(s) 330, 587, 647, 689, 2046
SmaI CCCGGG 1 cut(s) 465
SmiMI CAYNNNNRTG 2 cut(s) 735, 1268
SmlI CTYRAG 3 cut(s) 561, 798, 1115
SmoI CTYRAG 3 cut(s) 561, 798, 1115
SphI GCATGC 3 cut(s) 1600, 1648, 2286
Sse8387I CCTGCAGG 1 cut(s) 163
SsiI CCGC 3 cut(s) 9, 351, 942
SspI AATATT 2 cut(s) 922, 1762
SstI GAGCTC 1 cut(s) 144
StyD4I CCNGG 7 cut(s) 286, 462, 463, 590, 782, 1091, 2221
StyI CCWWGG 2 cut(s) 650, 1549
TaaI ACNGT 6 cut(s) 220, 325, 508, 881, 1148, 1837
TaqI TCGA 4 cut(s) 178, 831, 2111, 2179
TatI WGTACW 3 cut(s) 83, 438, 1056
TauI GCSGC 2 cut(s) 12, 945
Tru1I TTAA 5 cut(s) 794, 860, 1098, 1416, 1782
Tru9I TTAA 5 cut(s) 794, 860, 1098, 1416, 1782
TscAI CASTG 6 cut(s) 259, 286, 369, 415, 676, 1246
TseFI GTSAC 3 cut(s) 410, 881, 1895
TseI GCWGC 4 cut(s) 50, 814, 945, 1274
Tsp45I GTSAC 3 cut(s) 410, 881, 1895
TspMI CCCGGG 1 cut(s) 463
TspRI CASTG 6 cut(s) 259, 286, 369, 415, 676, 1246
Van91I CCANNNNNTGG 2 cut(s) 327, 656
VneI GTGCAC 1 cut(s) 1135
VpaK11BI GGWCC 5 cut(s) 330, 587, 647, 689, 2046
XagI CCTNNNNNAGG 2 cut(s) 561, 684
XapI RAATTY 5 cut(s) 291, 968, 1070, 1168, 1778
XbaI TCTAGA 2 cut(s) 1721, 2276
XceI RCATGY 6 cut(s) 1600, 1648, 1879, 2021, 2167, 2286
XmaI CCCGGG 1 cut(s) 463
XmnI GAANNNNTTC 2 cut(s) 609, 1147
ZrmI AGTACT 2 cut(s) 440, 1058
Zsp2I ATGCAT 2 cut(s) 1598, 2023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.