Rroxscaffold_1G00052990

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000484
Physical Location & Seq
Reverse (-)
73764849 .. 73770152
5304 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00052990.1

Sequence Viewer

Length: 2634 bp
ATGAAAGGGATAGAGAAAAGGATGGGAATTGACTTCAAGAATAATGCCAAAGAGTACCCAAATCAGAAATCTGCAGTTTTGTTAGAGCTGAGGGATTTGGATGACAAGATTTGTCCTGTGGAGTTCGGTATTTCCAGAACGCGCCGTGTTAGTGGACTGGCGATATTTTCCACAGTGGGAGGCAGCACAAGTACATTTCAGTCAATTAGAGATGGCCAGACTATAGTTTCAACTACTGGAAACTTTGAGCTTGGATTTTTTAGTCCTTATACTGGTTCTAGTAACCGATACGTTGGGATATGGTACAAGAAGATATCGGCTACAATAGTATGGGTTGCCAACAGAGAAGCACCCCTTACTGATTCATCAGGTATTCTGCGGGTCACCAACCCTGGAATTCTTGTCCTCCTCAACCATAACAAGAGCATAGTTTGGTCCTCAAACACATCGAGATCTGCAGATAATCCAGTGGCCCAGCTTTTGGATTCAGGAAATCTTGTTGTAACAGATGGCAATGATACAGACCCTGAGAACTTCTTGTGGCAAAGTTTTGATTACCCAAGTGATACACTCCTACCAGGTATGAAGCTTGGTTGGAACAAAGTTACAGGCTTCAGTAGGCATCTTACATCATGGAACAGCCCACAGGATCCTTCTCTAGGAAAATATACATATGAACTTGATCGCAGAGGATATGGAGAAGAAATTTTGAAGAAGGATTCAGTTATGATATGGCGAACCGGACCATGGAATGGAATCCGATTCAGTGGCACGCCTCATTTAACTCCAAACTCCATATTCACATACAATCTTGTTTTTGATGATCATGATGAAGAGTATTATATTTTTGAGCTTCTTAACAGCTCAGTTTATTCAAGAGTGATTCTCACTCCAGATGGGCGTGTCCAGCGCTACACATGGATTGATACAACCCAAACTTGGGGATTCCTTTATCAAACAGCTTTAATTGATAACTGTGACAATTATGCATTCTGTGGTCCAAATGGTGCTTGCAGTATTGACAGTTCCCCAGTATGTCGCTGCTTGAATGGATTTCAACCCAAATATCCAAAGGACTGGGATGTAGTGGATTGGTCAAATGGCTGTGTGAGAAGGACTCCACTAAATTCAGTAAATTGCAGCGGTGACTTGTTTGAGAAGTACTCTGCAGTGAAATTGCCAAACACAGAGCAGTCCTGGTTCAACAGAAGTATGAATCTCAAGGAATGTGAGATGGTGTGCCTGAAGAACTGTTCCTGCACAGCTTATGCAAATTTGGATATCAAGGATGGAGGATCTGGCTGCTTGCTGTGGTTTGGAAGCCTAATTGATGTTAGAAATTTAACTGTAAATGGGCAAGATATTTATATAAGAATGGCTGCATCAGAACTAGGCCTGGATCATGACAACACAGTTATCAATGCTAAAGACTCTGAATCTAAGAAGGTTAGAATCATATTATGTGGTACTCTGTTACCTACTGGACTGCTGATCGTGGGCATAGCCCTGGTATTTTATTGCCGGAAGAAGCAGCACAAAAAATATGGGAAAATGAGGAGCAGCAAAAAAGAAGATTCAGAACTTACCTCAAGAGAAGAAGGTTTAGAACTTCCCTCAAAAAAGGAAGATTTTGAACTTCCATTATTTGACTTGGCTACTGTCGTTTTTGCCACTGATAACTTCTCAGAGAACAGCAAACTTGGAGAAGGTGGATTCGGATCCGTCTTTAAGGGTATATTGCAAGATGGACAGGAAATAGCTGTGAAGAGGCTCTCGAAGCATTCTAATCAAGGACTGGATGAGTTCAAGAATGAGGTGACACATATCGTCAAACTTCAGCACAGGAATCTAGTGAAGCTTCTGGGATGCTGTATTCAGGAAGATGAAATGATGCTGATCTACGAATGCATGCCTAACAAAAGCTTGGACTTCTTTATTTTTGATCAAAGAAGAAGCTTTTTACTAGATTGGCCTAAGCGCTTTGACATTATTAATGGTATTGCTCGGGGGATCCTTTATCTTCATCAAGATTCTAGACTGAGGGTAATTCATAGAGATCTCAAAGCAGGCAATATCTTATTAGACAGTGAATTCAACCCAAAAATCTCAGACTTTGGCCTGGCTAGAAGTTTTGGAGGAAATGAAACTAAAGCAGAAACGAATAAAGTGGTCGGAACATATGGTTACATGTCCCCAGAATATGCAATTGATGGGTTCTACTCGATAAAATCAGACGTGTATAGCTTTGGTGTTTTGATGCTGGAGATAATTAGTGGGAAGAGAAACAGAGGATTTCTTCATCCAGGCCACAACCTCAACCTGCTTGGACATGCATGGAACTTACACACAGAAGGAAGGTCCATTGAATTGCTTGCTACATCAGTGGGGGACTCCATTAATCTACAAGAAGTTCTGCGAACGATTCATGTGGGTCTTTTATGTGTTCAGCAAAATCCACAAGACAGGCCAAGCATGTCAGCTGCAGTTCTAATGTTAAGTGGTGAAGGTGTGCTGCCTGTACCTCAGAAACCTGGTTTTTATAATGAGAGGGCTCTAACTGAACTCCAAGTTCATTCTTCTTCACAAGCATGTTCAGTTAATATACTCACCTCCACCCTATTCGAGCCTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

877

Amino Acids

98.48

Weight (kDa)

6.32

Isoelectric Point (pI)

45.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 108 - 212 2.6e-40 D-mannose binding lectin
S_locus_glycop PF00954 245 - 355 2.7e-29 S-locus glycoprotein domain
PAN_2 PF08276 380 - 445 1.9e-20 PAN-like domain
Pkinase PF00069 562 - 761 7.4e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 564 - 827 1.6e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2541
Acc36I ACCTGC 1 cut(s) 2328
AccB7I CCANNNNNTGG 2 cut(s) 481, 752
AccII CGCG 1 cut(s) 142
AciI CCGC 2 cut(s) 379, 1143
AclWI GGATC 8 cut(s) 644, 657, 1303, 1407, 1713, 1726, 2005, 2018
AcoI YGGCCR 1 cut(s) 214
AcsI RAATTY 6 cut(s) 396, 705, 1126, 1273, 1339, 2090
AcuI CTGAAG 3 cut(s) 598, 1265, 1820
AfaI GTAC 6 cut(s) 56, 193, 305, 1163, 1468, 2520
AfeI AGCGCT 2 cut(s) 911, 1979
AfiI CCNNNNNNNGG 7 cut(s) 272, 481, 659, 747, 752, 939, 940
AflIII ACRYGT 2 cut(s) 2187, 2235
AjiI CACGTC 1 cut(s) 2236
AjnI CCWGG 8 cut(s) 391, 577, 1196, 1395, 1506, 2118, 2302, 2530
AlwI GGATC 8 cut(s) 644, 657, 1303, 1407, 1713, 1726, 2005, 2018
AlwNI CAGNNNCTG 1 cut(s) 527
Ama87I CYCGRG 1 cut(s) 2004
Aor51HI AGCGCT 2 cut(s) 911, 1979
AoxI GGCC 7 cut(s) 214, 471, 1393, 1970, 2116, 2305, 2465
ApeKI GCWGC 9 cut(s) 183, 1041, 1140, 1302, 1379, 1531, 1560, 2480, 2512
ApoI RAATTY 6 cut(s) 396, 705, 1126, 1273, 1339, 2090
AseI ATTAAT 2 cut(s) 1992, 2397
AspLEI GCGC 3 cut(s) 144, 912, 1980
AspS9I GGNCC 5 cut(s) 435, 472, 743, 998, 2358
AsuHPI GGTGA 5 cut(s) 376, 1157, 1828, 2513, 2599
AvaI CYCGRG 1 cut(s) 2004
AvaII GGWCC 4 cut(s) 435, 743, 998, 2358
BalI TGGCCA 1 cut(s) 216
BamHI GGATCC 3 cut(s) 649, 1718, 2010
BanII GRGCYC 1 cut(s) 2554
BbvCI CCTCAGC 1 cut(s) 89
BbvI GCAGC 9 cut(s) 195, 1028, 1152, 1289, 1366, 1543, 1572, 2467, 2499
BccI CCATC 8 cut(s) 16, 206, 503, 890, 1228, 1283, 1739, 2204
BceAI ACGGC 1 cut(s) 129
BciT130I CCWGG 8 cut(s) 393, 579, 1198, 1397, 1508, 2120, 2304, 2532
BclI TGATCA 2 cut(s) 823, 1942
BfaI CTAG 7 cut(s) 279, 659, 1391, 1850, 1964, 2034, 2124
BfmI CTRYAG 5 cut(s) 72, 222, 456, 1167, 2481
BfoI RGCGCY 2 cut(s) 913, 1981
BfuAI ACCTGC 1 cut(s) 2328
BglII AGATCT 2 cut(s) 452, 2056
BisI GCNGC 9 cut(s) 184, 1042, 1141, 1303, 1380, 1532, 1561, 2481, 2513
BlsI GCNGC 9 cut(s) 185, 1043, 1142, 1304, 1381, 1533, 1562, 2482, 2514
BmcAI AGTACT 1 cut(s) 1163
Bme1390I CCNGG 8 cut(s) 393, 579, 1198, 1397, 1508, 2120, 2304, 2532
Bme18I GGWCC 4 cut(s) 435, 743, 998, 2358
BmeT110I CYCGRG 1 cut(s) 2004
BmgBI CACGTC 1 cut(s) 2236
BmgT120I GGNCC 5 cut(s) 435, 472, 743, 998, 2358
BmiI GGNNCC 3 cut(s) 651, 1720, 2012
BmrFI CCNGG 8 cut(s) 393, 579, 1198, 1397, 1508, 2120, 2304, 2532
BmrI ACTGGG 2 cut(s) 1025, 1087
BmsI GCATC 5 cut(s) 631, 1391, 1856, 1881, 2247
BmuI ACTGGG 2 cut(s) 1025, 1087
BplI GAGNNNNNCTC 6 cut(s) 870, 902, 1102, 1134, 1148, 1180
BpmI CTGGAG 2 cut(s) 876, 2282
Bpu10I CCTNAGC 2 cut(s) 89, 1974
BpuEI CTTGAG 2 cut(s) 1205, 1573
BsaBI GATNNNNATC 2 cut(s) 1717, 1895
BsaJI CCNNGG 3 cut(s) 391, 746, 1506
BsaWI WCCGGW 1 cut(s) 740
Bsc4I CCNNNNNNNGG 7 cut(s) 272, 481, 659, 747, 752, 939, 940
Bse1I ACTGG 8 cut(s) 162, 241, 277, 467, 1031, 1082, 1486, 1800
Bse3DI GCAATG 1 cut(s) 520
Bse8I GATNNNNATC 2 cut(s) 1717, 1895
BseBI CCWGG 8 cut(s) 393, 579, 1198, 1397, 1508, 2120, 2304, 2532
BseDI CCNNGG 3 cut(s) 391, 746, 1506
BseGI GGATG 7 cut(s) 27, 106, 1087, 1294, 1804, 1871, 2299
BseJI GATNNNNATC 2 cut(s) 1717, 1895
BseLI CCNNNNNNNGG 7 cut(s) 272, 481, 659, 747, 752, 939, 940
BseMI GCAATG 1 cut(s) 520
BseMII CTCAG 7 cut(s) 80, 519, 879, 1698, 2030, 2121, 2537
BseNI ACTGG 8 cut(s) 162, 241, 277, 467, 1031, 1082, 1486, 1800
BseRI GAGGAG 2 cut(s) 398, 1570
BseXI GCAGC 9 cut(s) 195, 1028, 1152, 1289, 1366, 1543, 1572, 2467, 2499
BseYI CCCAGC 1 cut(s) 474
BsgI GTGCAG 1 cut(s) 1243
Bsh1236I CGCG 1 cut(s) 142
BshFI GGCC 7 cut(s) 216, 473, 1395, 1972, 2118, 2307, 2467
BsiHKCI CYCGRG 1 cut(s) 2004
BsiSI CCGG 2 cut(s) 741, 1522
BslFI GGGAC 2 cut(s) 2176, 2402
BslI CCNNNNNNNGG 7 cut(s) 272, 481, 659, 747, 752, 939, 940
BsmFI GGGAC 2 cut(s) 2176, 2402
BsmI GAATGC 3 cut(s) 989, 1780, 1910
BsnI GGCC 7 cut(s) 216, 473, 1395, 1972, 2118, 2307, 2467
BsoBI CYCGRG 1 cut(s) 2004
Bsp1286I GDGCHC 1 cut(s) 2554
Bsp19I CCATGG 1 cut(s) 746
BspACI CCGC 2 cut(s) 379, 1143
BspANI GGCC 7 cut(s) 216, 473, 1395, 1972, 2118, 2307, 2467
BspCNI CTCAG 7 cut(s) 81, 520, 878, 1697, 2031, 2120, 2536
BspFNI CGCG 1 cut(s) 142
BspHI TCATGA 2 cut(s) 826, 1402
BspLI GGNNCC 3 cut(s) 651, 1720, 2012
BspMAI CTGCAG 4 cut(s) 76, 460, 1171, 2485
BspMI ACCTGC 1 cut(s) 2328
BspPI GGATC 8 cut(s) 644, 657, 1303, 1407, 1713, 1726, 2005, 2018
BsrDI GCAATG 1 cut(s) 520
BsrI ACTGG 8 cut(s) 162, 241, 277, 467, 1031, 1082, 1486, 1800
BssECI CCNNGG 3 cut(s) 391, 746, 1506
BssT1I CCWWGG 1 cut(s) 746
Bst2UI CCWGG 8 cut(s) 393, 579, 1198, 1397, 1508, 2120, 2304, 2532
Bst4CI ACNGT 8 cut(s) 175, 977, 1025, 1253, 1348, 1414, 1660, 2087
Bst6I CTCTTC 3 cut(s) 828, 1760, 2273
BstC8I GCNNGC 6 cut(s) 773, 1012, 1307, 1910, 2068, 2373
BstDEI CTNAG 9 cut(s) 89, 528, 865, 1440, 1684, 1974, 2039, 2107, 2523
BstDSI CCRYGG 1 cut(s) 746
BstEII GGTNACC 1 cut(s) 382
BstENI CCTNNNNNAGG 1 cut(s) 657
BstF5I GGATG 7 cut(s) 27, 106, 1087, 1294, 1804, 1871, 2299
BstFNI CGCG 1 cut(s) 142
BstH2I RGCGCY 2 cut(s) 913, 1981
BstHHI GCGC 3 cut(s) 144, 912, 1980
BstMWI GCNNNNNNNGC 2 cut(s) 907, 1777
BstNI CCWGG 8 cut(s) 393, 579, 1198, 1397, 1508, 2120, 2304, 2532
BstNSI RCATGY 5 cut(s) 1912, 2191, 2333, 2476, 2592
BstPI GGTNACC 1 cut(s) 382
BstSCI CCNGG 8 cut(s) 391, 577, 1196, 1395, 1506, 2118, 2302, 2530
BstSFI CTRYAG 5 cut(s) 72, 222, 456, 1167, 2481
BstUI CGCG 1 cut(s) 142
BstV1I GCAGC 9 cut(s) 195, 1028, 1152, 1289, 1366, 1543, 1572, 2467, 2499
BstX2I RGATCY 6 cut(s) 452, 649, 1295, 1718, 2010, 2056
BstXI CCANNNNNNTGG 1 cut(s) 1077
BstYI RGATCY 6 cut(s) 452, 649, 1295, 1718, 2010, 2056
BsuRI GGCC 7 cut(s) 216, 473, 1395, 1972, 2118, 2307, 2467
BtgI CCRYGG 1 cut(s) 746
BtrI CACGTC 1 cut(s) 2236
BtsCI GGATG 7 cut(s) 27, 106, 1087, 1294, 1804, 1871, 2299
BtsI GCAGTG 1 cut(s) 1176
BtsIMutI CAGTG 7 cut(s) 180, 474, 772, 1176, 1671, 2092, 2388
BveI ACCTGC 1 cut(s) 2328
Cac8I GCNNGC 6 cut(s) 773, 1012, 1307, 1910, 2068, 2373
CaiI CAGNNNCTG 1 cut(s) 527
CciI TCATGA 2 cut(s) 826, 1402
CfoI GCGC 3 cut(s) 144, 912, 1980
Cfr13I GGNCC 5 cut(s) 435, 472, 743, 998, 2358
CsiI ACCWGGT 2 cut(s) 577, 2530
Csp6I GTAC 6 cut(s) 55, 192, 304, 1162, 1467, 2519
CviQI GTAC 6 cut(s) 55, 192, 304, 1162, 1467, 2519
DdeI CTNAG 9 cut(s) 89, 528, 865, 1440, 1684, 1974, 2039, 2107, 2523
EaeI YGGCCR 1 cut(s) 214
Eam1104I CTCTTC 3 cut(s) 828, 1760, 2273
EarI CTCTTC 3 cut(s) 828, 1760, 2273
Eco130I CCWWGG 1 cut(s) 746
Eco147I AGGCCT 1 cut(s) 1395
Eco24I GRGCYC 1 cut(s) 2554
Eco32I GATATC 2 cut(s) 315, 1282
Eco47I GGWCC 4 cut(s) 435, 743, 998, 2358
Eco47III AGCGCT 2 cut(s) 911, 1979
Eco57I CTGAAG 3 cut(s) 598, 1265, 1820
Eco88I CYCGRG 1 cut(s) 2004
Eco91I GGTNACC 1 cut(s) 382
EcoNI CCTNNNNNAGG 1 cut(s) 657
EcoO65I GGTNACC 1 cut(s) 382
EcoRI GAATTC 2 cut(s) 396, 2090
EcoRII CCWGG 8 cut(s) 391, 577, 1196, 1395, 1506, 2118, 2302, 2530
EcoRV GATATC 2 cut(s) 315, 1282
EcoT14I CCWWGG 1 cut(s) 746
EcoT22I ATGCAT 3 cut(s) 991, 1910, 2335
EcoT38I GRGCYC 1 cut(s) 2554
ErhI CCWWGG 1 cut(s) 746
FalI AAGNNNNNCTT 2 cut(s) 339, 371
FaqI GGGAC 2 cut(s) 2176, 2402
FauI CCCGC 1 cut(s) 372
FauNDI CATATG 2 cut(s) 673, 2179
FbaI TGATCA 2 cut(s) 823, 1942
Fnu4HI GCNGC 9 cut(s) 184, 1042, 1141, 1303, 1380, 1532, 1561, 2481, 2513
FokI GGATG 7 cut(s) 34, 113, 1094, 1301, 1811, 1878, 2286
FriOI GRGCYC 1 cut(s) 2554
Fsp4HI GCNGC 9 cut(s) 184, 1042, 1141, 1303, 1380, 1532, 1561, 2481, 2513
FspBI CTAG 7 cut(s) 279, 659, 1391, 1850, 1964, 2034, 2124
GlaI GCGC 3 cut(s) 143, 911, 1979
GluI GCNGC 9 cut(s) 184, 1042, 1141, 1303, 1380, 1532, 1561, 2481, 2513
GsaI CCCAGC 1 cut(s) 478
GsuI CTGGAG 2 cut(s) 876, 2282
HaeII RGCGCY 2 cut(s) 913, 1981
HaeIII GGCC 7 cut(s) 216, 473, 1395, 1972, 2118, 2307, 2467
HapII CCGG 2 cut(s) 741, 1522
HhaI GCGC 3 cut(s) 144, 912, 1980
Hin6I GCGC 3 cut(s) 142, 910, 1978
HinP1I GCGC 3 cut(s) 142, 910, 1978
HindIII AAGCTT 4 cut(s) 587, 1856, 1921, 1954
HpaII CCGG 2 cut(s) 741, 1522
HphI GGTGA 5 cut(s) 376, 1157, 1828, 2513, 2599
Hpy166II GTNNAC 1 cut(s) 155
Hpy8I GTNNAC 1 cut(s) 155
HpyAV CCTTC 9 cut(s) 663, 709, 1107, 1438, 1592, 1700, 2345, 2349, 2498
HpyCH4III ACNGT 8 cut(s) 175, 977, 1025, 1253, 1348, 1414, 1660, 2087
HpyCH4IV ACGT 2 cut(s) 291, 2235
HpyF10VI GCNNNNNNNGC 2 cut(s) 907, 1777
HpyF3I CTNAG 9 cut(s) 89, 528, 865, 1440, 1684, 1974, 2039, 2107, 2523
HpySE526I ACGT 2 cut(s) 291, 2235
HspAI GCGC 3 cut(s) 142, 910, 1978
Ksp22I TGATCA 2 cut(s) 823, 1942
LmnI GCTCC 1 cut(s) 1557
Lsp1109I GCAGC 9 cut(s) 195, 1028, 1152, 1289, 1366, 1543, 1572, 2467, 2499
LweI GCATC 5 cut(s) 631, 1391, 1856, 1881, 2247
MabI ACCWGGT 2 cut(s) 577, 2530
MaeI CTAG 7 cut(s) 279, 659, 1391, 1850, 1964, 2034, 2124
MaeII ACGT 2 cut(s) 291, 2235
MaeIII GTNAC 9 cut(s) 281, 382, 502, 604, 977, 1145, 1473, 1816, 2183
MfeI CAATTG 1 cut(s) 2205
MflI RGATCY 6 cut(s) 452, 649, 1295, 1718, 2010, 2056
MhlI GDGCHC 1 cut(s) 2554
MlsI TGGCCA 1 cut(s) 216
MluNI TGGCCA 1 cut(s) 216
MlyI GAGTC 3 cut(s) 1111, 1424, 2384
MmeI TCCRAC 2 cut(s) 575, 2152
Mox20I TGGCCA 1 cut(s) 216
Mph1103I ATGCAT 3 cut(s) 991, 1910, 2335
MscI TGGCCA 1 cut(s) 216
MslI CAYNNNNRTG 1 cut(s) 2587
Msp20I TGGCCA 1 cut(s) 216
MspA1I CMGCKG 2 cut(s) 1143, 2480
MspI CCGG 2 cut(s) 741, 1522
MspR9I CCNGG 8 cut(s) 393, 579, 1198, 1397, 1508, 2120, 2304, 2532
MunI CAATTG 1 cut(s) 2205
Mva1269I GAATGC 3 cut(s) 989, 1780, 1910
MvaI CCWGG 8 cut(s) 393, 579, 1198, 1397, 1508, 2120, 2304, 2532
MvnI CGCG 1 cut(s) 142
MwoI GCNNNNNNNGC 2 cut(s) 907, 1777
NcoI CCATGG 1 cut(s) 746
NdeI CATATG 2 cut(s) 673, 2179
NlaIV GGNNCC 3 cut(s) 651, 1720, 2012
NmuCI GTSAC 4 cut(s) 382, 977, 1145, 1816
NsiI ATGCAT 3 cut(s) 991, 1910, 2335
NspI RCATGY 5 cut(s) 1912, 2191, 2333, 2476, 2592
PaeI GCATGC 1 cut(s) 1912
PagI TCATGA 2 cut(s) 826, 1402
PceI AGGCCT 1 cut(s) 1395
PciI ACATGT 1 cut(s) 2187
PctI GAATGC 3 cut(s) 989, 1780, 1910
PflMI CCANNNNNTGG 2 cut(s) 481, 752
PkrI GCNGC 9 cut(s) 185, 1043, 1142, 1304, 1381, 1533, 1562, 2482, 2514
PleI GAGTC 3 cut(s) 1111, 1424, 2384
PpsI GAGTC 3 cut(s) 1111, 1424, 2384
PscI ACATGT 1 cut(s) 2187
PshBI ATTAAT 2 cut(s) 1992, 2397
PsiI TTATAA 1 cut(s) 2541
Psp6I CCWGG 8 cut(s) 391, 577, 1196, 1395, 1506, 2118, 2302, 2530
PspEI GGTNACC 1 cut(s) 382
PspFI CCCAGC 1 cut(s) 474
PspGI CCWGG 8 cut(s) 391, 577, 1196, 1395, 1506, 2118, 2302, 2530
PspN4I GGNNCC 3 cut(s) 651, 1720, 2012
PspPI GGNCC 5 cut(s) 435, 472, 743, 998, 2358
PsrI GAACNNNNNNTAC 2 cut(s) 1009, 1041
PstI CTGCAG 4 cut(s) 76, 460, 1171, 2485
PstNI CAGNNNCTG 1 cut(s) 527
PsuI RGATCY 6 cut(s) 452, 649, 1295, 1718, 2010, 2056
PvuII CAGCTG 1 cut(s) 2480
RsaI GTAC 6 cut(s) 56, 193, 305, 1163, 1468, 2520
RsaNI GTAC 6 cut(s) 55, 192, 304, 1162, 1467, 2519
RseI CAYNNNNRTG 1 cut(s) 2587
SatI GCNGC 9 cut(s) 184, 1042, 1141, 1303, 1380, 1532, 1561, 2481, 2513
Sau96I GGNCC 5 cut(s) 435, 472, 743, 998, 2358
ScaI AGTACT 1 cut(s) 1163
SchI GAGTC 3 cut(s) 1111, 1424, 2384
ScrFI CCNGG 8 cut(s) 393, 579, 1198, 1397, 1508, 2120, 2304, 2532
SduI GDGCHC 1 cut(s) 2554
SexAI ACCWGGT 2 cut(s) 577, 2530
SfaNI GCATC 5 cut(s) 631, 1391, 1856, 1881, 2247
SfcI CTRYAG 5 cut(s) 72, 222, 456, 1167, 2481
SinI GGWCC 4 cut(s) 435, 743, 998, 2358
SmiMI CAYNNNNRTG 1 cut(s) 2587
SmlI CTYRAG 2 cut(s) 1220, 1588
SmoI CTYRAG 2 cut(s) 1220, 1588
SphI GCATGC 1 cut(s) 1912
SseBI AGGCCT 1 cut(s) 1395
SsiI CCGC 2 cut(s) 379, 1143
SspMI CTAG 7 cut(s) 279, 659, 1391, 1850, 1964, 2034, 2124
StuI AGGCCT 1 cut(s) 1395
StyD4I CCNGG 8 cut(s) 391, 577, 1196, 1395, 1506, 2118, 2302, 2530
StyI CCWWGG 1 cut(s) 746
TaaI ACNGT 8 cut(s) 175, 977, 1025, 1253, 1348, 1414, 1660, 2087
TaiI ACGT 2 cut(s) 294, 2238
TaqI TCGA 4 cut(s) 449, 1775, 2222, 2622
TatI WGTACW 2 cut(s) 191, 1161
TscAI CASTG 7 cut(s) 180, 474, 772, 1176, 1678, 2092, 2388
TseFI GTSAC 4 cut(s) 382, 977, 1145, 1816
TseI GCWGC 9 cut(s) 183, 1041, 1140, 1302, 1379, 1531, 1560, 2480, 2512
Tsp45I GTSAC 4 cut(s) 382, 977, 1145, 1816
TspGWI ACGGA 1 cut(s) 1711
TspRI CASTG 7 cut(s) 180, 474, 772, 1176, 1678, 2092, 2388
Van91I CCANNNNNTGG 2 cut(s) 481, 752
VpaK11BI GGWCC 4 cut(s) 435, 743, 998, 2358
VspI ATTAAT 2 cut(s) 1992, 2397
XagI CCTNNNNNAGG 1 cut(s) 657
XapI RAATTY 6 cut(s) 396, 705, 1126, 1273, 1339, 2090
XbaI TCTAGA 1 cut(s) 2033
XceI RCATGY 5 cut(s) 1912, 2191, 2333, 2476, 2592
XspI CTAG 7 cut(s) 279, 659, 1391, 1850, 1964, 2034, 2124
ZrmI AGTACT 1 cut(s) 1163
Zsp2I ATGCAT 3 cut(s) 991, 1910, 2335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.