RLG00000032928

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000484
Physical Location & Seq
Forward (+)
21031314 .. 21034398
3085 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032928

Sequence Viewer

Length: 2169 bp
ATGCTTGCCGCAGCAGAAGACAGCACAAGTACATTTCAGTCCATCAAAGACGGCGAGACCATAGTTTCAACCGATGGAACCTTTGAGCTCGGATTCTTCACTCCTGCAGGCGCTTCTGGTAATCGATACGTAGGCATATGGTACAAGAAGATATCGGTTATAACAGTTGTGTGGGTTGCCAACAGAGAAACACCCCTCACTGATTCATCAGGCATTCTCAAAATCACTAACCCTGGAATTCTTGTCCTTGTCAACCAGAACAAGACCACAGTTTGGTCCACCAACACATCAACAACCACACCGAATGCAGTGGCACAGCTTTTGGATTCAGGAAACCTTGTTGTGAAAGATGGCAGTGACAAAGTTGATCCAGAGAATGAGAAGTACTTGTGGCAGAGTTTTAACTACCCTGGTGATACTTTGCTATCAGGTATGAAGCTTGGTAGGAACACAGTTACAGGCTTCAATTGGCATCTTACATCATGGAGAAGTCCCTCTGATCCTTCTCAAGGCAACTTCACATTTCAACATGGTCCCAGAGGATATGCAGAACAAGTTCTGAGGGAGGGTTCAGACATAAGATTTCGGACTGGACCATGGAATGGAATCCGATTCAGTGGAACGCCTCATTTAGGTCCAAACTATGTTTACACATATCAGCTAGTTTTTGATGATCATGATCATGAAGAATACTATAGCTATAAGCTTCTCAACAGCTCAGTTCTTTCCAGGCTGGTGTTAACTCAAGATGGGCTTCTGCGGCGCTACACATGGATTGATAGAACCCAAAGTTGGTTCCTTTACTTAACAGCACAGATGGATAACTGTGACAATTATGCACTTTGTGGTGCATATGGTGCTTGCAATATTGAGAACTCCCCAGTATGTGGCTGCTTGAAAGGTTTTGTGCCAAAATTTCCAAAAGATTGGGACACAATGGATTGGTCAAATGGGTGTGTGAGAAAAACTCCACTAGATTGCAATGGCGACAAGTTTCTGAAGTACTCTGGGGTCAAATTTCCAAACACAGAACAATCCTGGTTTAACAAAAGTATGAGCCTCAAGGAATGCGAGATGGAGTGCACTAGAAACTGTTCCTGCACTGCTTATTCAAATTTGTATATTAGCGAAGGAGGAACTGGGTGCTTGATGTGGTTTGGCGACCTGATTGATATGAGAAATCTCACTGAAAATGGGCAAGATATTTACATAAGGATGGCAGCATCAGAACAAGGTATGGAGTCATCTTCAACCCAAAAAGGTATGTTGAGAAATGGGCAAGAAATAGCTGTCAAAAGGCTCTCAAAACATTCTTCACAAGGAGTAAATGAGTTCAAAAATGAGGTTACGCATATTGCCAAACTCCAGCACAGGAATCTAGTGAAGCTCCTTGGATGTTGCATTCAAGCTGATGAAATGATGCTCATCTACGAATGCATGCCTAACAAAAGCTTGGACTTCTTTATTTTTGATCAAAGAAAAAGCATGCTACTAGATTGGCCTAAGCGCTTTGACATCATCAATGGTATTGCTCGGGGGCTCCTCTATCTTCATCAAGATTCTAGACTGAGAGTAATTCATAGAGATCTCAAAGCAGGCAATATTTTATTAGACAATGAATTTAACCCCAAGATCTCAGACTTTGGTCTAGCTAGAAGTTTTGGAGGAAATGAAACTGTAGCAGAAACGATGAGAGTGGTTGGAACCTATGGTTACATGTCCCCAGAATATGCAAGTGACGGATTCTACTCTACCAAATCTGATGTTTTTAGCTTTGGTGTTTCGGTGTTGGAGATAATAAGTGGGAAGAGAAACAGAGGATTCTCTCATCCAGACCACGACCTCAACCTTCTTGGACATGCATGGAATCTATACACAGAAGGCAGGTCCATAGAATTGCTTGATACATCAGTAGGGGACTCCACTAATCCATCCGAGGAAGTTCTGCGCTCGATTCATGTGGGTCTTTTATGCGTGCAGAAAGATCCAGAAGATAGGCCAAGCATGTCAGATGTTGTTCTAATGTTGAGTGGTGAAGGTGCATTGCCTCAACCTGAAAAACCGGGGTTTTATAGTAAAAGCAGTGATAGGTCTGAAAACCAAGTCAATCCTTTATCTAGAGCATGCTCAGCTAATGAAGTTAGTTTTACGCTACTTGAGGCTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

723

Amino Acids

81.01

Weight (kDa)

5.64

Isoelectric Point (pI)

41.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 55 - 163 2.1e-37 D-mannose binding lectin
S_locus_glycop PF00954 195 - 306 6.4e-33 S-locus glycoprotein domain
PAN_2 PF08276 327 - 392 3.8e-20 PAN-like domain
Pkinase PF00069 419 - 673 4.8e-40 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 424 - 674 5.5e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 161
Acc36I ACCTGC 1 cut(s) 1875
AccB7I CCANNNNNTGG 3 cut(s) 273, 602, 887
AciI CCGC 2 cut(s) 9, 760
AclWI GGATC 3 cut(s) 362, 494, 1979
AcsI RAATTY 5 cut(s) 237, 914, 1016, 1114, 1619
AcuI CTGAAG 1 cut(s) 1019
AdeI CACNNNGTG 1 cut(s) 845
AfaI GTAC 4 cut(s) 31, 143, 386, 1004
AfeI AGCGCT 1 cut(s) 1508
AfiI CCNNNNNNNGG 6 cut(s) 273, 509, 602, 632, 792, 887
AflIII ACRYGT 1 cut(s) 1716
AgsI TTSAA 8 cut(s) 69, 466, 527, 898, 1113, 1251, 1336, 1406
AjnI CCWGG 4 cut(s) 232, 409, 728, 1037
AjuI GAANNNNNNNTTGG 2 cut(s) 775, 807
Alw21I GWGCWC 2 cut(s) 90, 1085
Alw26I GTCTC 1 cut(s) 50
Alw44I GTGCAC 1 cut(s) 1081
AlwI GGATC 3 cut(s) 362, 494, 1979
Ama87I CYCGRG 1 cut(s) 1533
Aor51HI AGCGCT 1 cut(s) 1508
AoxI GGCC 2 cut(s) 1499, 1997
ApaLI GTGCAC 1 cut(s) 1081
ApeKI GCWGC 3 cut(s) 11, 891, 1220
ApoI RAATTY 5 cut(s) 237, 914, 1016, 1114, 1619
Asp700I GAANNNNTTC 2 cut(s) 555, 1093
AspLEI GCGC 4 cut(s) 113, 765, 1509, 1950
AspS9I GGNCC 5 cut(s) 276, 533, 593, 635, 1887
AsuC2I CCSGG 1 cut(s) 2064
AsuHPI GGTGA 2 cut(s) 425, 2045
AvaI CYCGRG 1 cut(s) 1533
AvaII GGWCC 5 cut(s) 276, 533, 593, 635, 1887
BaeGI GKGCMC 1 cut(s) 1085
BaeI ACNNNNGTAYC 2 cut(s) 1896, 1929
BanII GRGCYC 2 cut(s) 90, 1542
BbsI GAAGAC 1 cut(s) 24
Bbv12I GWGCWC 2 cut(s) 90, 1085
BbvI GCAGC 3 cut(s) 23, 878, 1232
BccI CCATC 8 cut(s) 50, 68, 344, 743, 811, 1069, 1210, 1939
BceAI ACGGC 1 cut(s) 67
BciT130I CCWGG 4 cut(s) 234, 411, 730, 1039
BclI TGATCA 3 cut(s) 673, 679, 1471
BcnI CCSGG 1 cut(s) 2064
BcoDI GTCTC 1 cut(s) 50
BfmI CTRYAG 3 cut(s) 105, 694, 1677
BfoI RGCGCY 3 cut(s) 114, 766, 1510
BfuAI ACCTGC 1 cut(s) 1875
BglII AGATCT 2 cut(s) 1585, 1632
BisI GCNGC 5 cut(s) 9, 12, 761, 892, 1221
BlpI GCTNAGC 1 cut(s) 2128
BlsI GCNGC 5 cut(s) 10, 13, 762, 893, 1222
BmcAI AGTACT 2 cut(s) 386, 1004
Bme1390I CCNGG 5 cut(s) 234, 411, 730, 1039, 2064
Bme18I GGWCC 5 cut(s) 276, 533, 593, 635, 1887
BmeT110I CYCGRG 1 cut(s) 1533
BmgT120I GGNCC 5 cut(s) 276, 533, 593, 635, 1887
BmiI GGNNCC 5 cut(s) 79, 535, 797, 1541, 1705
BmrFI CCNGG 5 cut(s) 234, 411, 730, 1039, 2064
BmrI ACTGGG 2 cut(s) 875, 1149
BmsI GCATC 3 cut(s) 481, 1232, 1410
BmuI ACTGGG 2 cut(s) 875, 1149
BoxI GACNNNNGTC 1 cut(s) 1644
BpiI GAAGAC 1 cut(s) 24
BplI GAGNNNNNCTC 2 cut(s) 952, 984
BpmI CTGGAG 1 cut(s) 1349
Bpu10I CCTNAGC 1 cut(s) 1503
Bpu1102I GCTNAGC 1 cut(s) 2128
BpuEI CTTGAG 3 cut(s) 492, 729, 1046
BpuMI CCSGG 1 cut(s) 2064
Bsa29I ATCGAT 1 cut(s) 124
BsaAI YACGTR 1 cut(s) 130
BsaBI GATNNNNATC 2 cut(s) 678, 1424
BsaI GGTCTC 1 cut(s) 50
BsaJI CCNNGG 6 cut(s) 232, 409, 596, 1390, 1935, 2063
Bsc4I CCNNNNNNNGG 6 cut(s) 273, 509, 602, 632, 792, 887
Bse1I ACTGG 3 cut(s) 595, 881, 1144
Bse3DI GCAATG 2 cut(s) 988, 2042
Bse8I GATNNNNATC 2 cut(s) 678, 1424
BseBI CCWGG 4 cut(s) 234, 411, 730, 1039
BseCI ATCGAT 1 cut(s) 124
BseDI CCNNGG 6 cut(s) 232, 409, 596, 1390, 1935, 2063
BseGI GGATG 4 cut(s) 1221, 1400, 1828, 1931
BseJI GATNNNNATC 2 cut(s) 678, 1424
BseLI CCNNNNNNNGG 6 cut(s) 273, 509, 602, 632, 792, 887
BseMI GCAATG 2 cut(s) 988, 2042
BseMII CTCAG 5 cut(s) 551, 732, 1559, 1650, 2142
BseNI ACTGG 3 cut(s) 595, 881, 1144
BseRI GAGGAG 1 cut(s) 1532
BseSI GKGCMC 1 cut(s) 1085
BseXI GCAGC 3 cut(s) 23, 878, 1232
BsgI GTGCAG 2 cut(s) 1084, 1997
BshFI GGCC 2 cut(s) 1501, 1999
BshVI ATCGAT 1 cut(s) 124
BsiHKAI GWGCWC 2 cut(s) 90, 1085
BsiHKCI CYCGRG 1 cut(s) 1533
BsiSI CCGG 1 cut(s) 2063
BslFI GGGAC 5 cut(s) 477, 519, 944, 1705, 1931
BslI CCNNNNNNNGG 6 cut(s) 273, 509, 602, 632, 792, 887
BsmAI GTCTC 1 cut(s) 50
BsmFI GGGAC 5 cut(s) 477, 519, 944, 1705, 1931
BsmI GAATGC 5 cut(s) 213, 310, 1073, 1401, 1439
BsnI GGCC 2 cut(s) 1501, 1999
Bso31I GGTCTC 1 cut(s) 50
BsoBI CYCGRG 1 cut(s) 1533
Bsp1286I GDGCHC 3 cut(s) 90, 1085, 1542
Bsp143I GATC 8 cut(s) 367, 499, 673, 679, 1471, 1585, 1632, 1984
Bsp1720I GCTNAGC 1 cut(s) 2128
Bsp19I CCATGG 1 cut(s) 596
BspACI CCGC 2 cut(s) 9, 760
BspANI GGCC 2 cut(s) 1501, 1999
BspCNI CTCAG 5 cut(s) 552, 731, 1560, 1649, 2141
BspDI ATCGAT 1 cut(s) 124
BspHI TCATGA 2 cut(s) 676, 682
BspLI GGNNCC 5 cut(s) 79, 535, 797, 1541, 1705
BspMAI CTGCAG 1 cut(s) 109
BspMI ACCTGC 1 cut(s) 1875
BspPI GGATC 3 cut(s) 362, 494, 1979
BspTNI GGTCTC 1 cut(s) 50
BsrDI GCAATG 2 cut(s) 988, 2042
BsrI ACTGG 3 cut(s) 595, 881, 1144
BssECI CCNNGG 6 cut(s) 232, 409, 596, 1390, 1935, 2063
BssMI GATC 8 cut(s) 367, 499, 673, 679, 1471, 1585, 1632, 1984
BssT1I CCWWGG 2 cut(s) 596, 1390
Bst2UI CCWGG 4 cut(s) 234, 411, 730, 1039
Bst4CI ACNGT 6 cut(s) 166, 271, 454, 827, 1094, 1678
Bst6I CTCTTC 1 cut(s) 1802
BstAPI GCANNNNNTGC 1 cut(s) 857
BstBAI YACGTR 1 cut(s) 130
BstC8I GCNNGC 8 cut(s) 6, 109, 862, 1439, 1487, 1597, 1976, 2125
BstDEI CTNAG 6 cut(s) 560, 718, 1503, 1568, 1636, 2128
BstDSI CCRYGG 1 cut(s) 596
BstENI CCTNNNNNAGG 2 cut(s) 507, 630
BstF5I GGATG 4 cut(s) 1221, 1400, 1828, 1931
BstH2I RGCGCY 3 cut(s) 114, 766, 1510
BstHHI GCGC 4 cut(s) 113, 765, 1509, 1950
BstKTI GATC 8 cut(s) 370, 502, 676, 682, 1474, 1588, 1635, 1987
BstMAI GTCTC 1 cut(s) 50
BstMBI GATC 8 cut(s) 367, 499, 673, 679, 1471, 1585, 1632, 1984
BstMWI GCNNNNNNNGC 3 cut(s) 760, 857, 2129
BstNI CCWGG 4 cut(s) 234, 411, 730, 1039
BstNSI RCATGY 6 cut(s) 1441, 1489, 1720, 1862, 2008, 2127
BstPAI GACNNNNGTC 1 cut(s) 1644
BstSCI CCNGG 5 cut(s) 232, 409, 728, 1037, 2062
BstSFI CTRYAG 3 cut(s) 105, 694, 1677
BstSLI GKGCMC 1 cut(s) 1085
BstSNI TACGTA 1 cut(s) 130
BstV1I GCAGC 3 cut(s) 23, 878, 1232
BstV2I GAAGAC 1 cut(s) 24
BstX2I RGATCY 3 cut(s) 1585, 1632, 1984
BstXI CCANNNNNNTGG 1 cut(s) 927
BstYI RGATCY 3 cut(s) 1585, 1632, 1984
Bsu15I ATCGAT 1 cut(s) 124
BsuRI GGCC 2 cut(s) 1501, 1999
BsuTUI ATCGAT 1 cut(s) 124
BtgI CCRYGG 1 cut(s) 596
BtsCI GGATG 4 cut(s) 1221, 1400, 1828, 1931
BtsI GCAGTG 4 cut(s) 315, 361, 1101, 2089
BtsIMutI CAGTG 7 cut(s) 198, 315, 361, 622, 1101, 1185, 2089
BveI ACCTGC 1 cut(s) 1875
Cac8I GCNNGC 8 cut(s) 6, 109, 862, 1439, 1487, 1597, 1976, 2125
CciI TCATGA 2 cut(s) 676, 682
CfoI GCGC 4 cut(s) 113, 765, 1509, 1950
Cfr13I GGNCC 5 cut(s) 276, 533, 593, 635, 1887
ClaI ATCGAT 1 cut(s) 124
Csp6I GTAC 4 cut(s) 30, 142, 385, 1003
CviQI GTAC 4 cut(s) 30, 142, 385, 1003
DdeI CTNAG 6 cut(s) 560, 718, 1503, 1568, 1636, 2128
DpnI GATC 8 cut(s) 369, 501, 675, 681, 1473, 1587, 1634, 1986
DpnII GATC 8 cut(s) 367, 499, 673, 679, 1471, 1585, 1632, 1984
DraIII CACNNNGTG 1 cut(s) 845
Eam1104I CTCTTC 1 cut(s) 1802
EarI CTCTTC 1 cut(s) 1802
Ecl136II GAGCTC 1 cut(s) 88
Eco105I TACGTA 1 cut(s) 130
Eco130I CCWWGG 2 cut(s) 596, 1390
Eco24I GRGCYC 2 cut(s) 90, 1542
Eco31I GGTCTC 1 cut(s) 50
Eco32I GATATC 1 cut(s) 153
Eco47I GGWCC 5 cut(s) 276, 533, 593, 635, 1887
Eco47III AGCGCT 1 cut(s) 1508
Eco53kI GAGCTC 1 cut(s) 88
Eco57I CTGAAG 1 cut(s) 1019
Eco88I CYCGRG 1 cut(s) 1533
EcoICRI GAGCTC 1 cut(s) 88
EcoNI CCTNNNNNAGG 2 cut(s) 507, 630
EcoRI GAATTC 1 cut(s) 237
EcoRII CCWGG 4 cut(s) 232, 409, 728, 1037
EcoRV GATATC 1 cut(s) 153
EcoT14I CCWWGG 2 cut(s) 596, 1390
EcoT22I ATGCAT 2 cut(s) 1439, 1864
EcoT38I GRGCYC 2 cut(s) 90, 1542
ErhI CCWWGG 2 cut(s) 596, 1390
FalI AAGNNNNNCTT 2 cut(s) 738, 770
FaqI GGGAC 5 cut(s) 477, 519, 944, 1705, 1931
FauNDI CATATG 2 cut(s) 137, 853
FbaI TGATCA 3 cut(s) 673, 679, 1471
Fnu4HI GCNGC 5 cut(s) 9, 12, 761, 892, 1221
FokI GGATG 4 cut(s) 1228, 1407, 1815, 1918
FriOI GRGCYC 2 cut(s) 90, 1542
Fsp4HI GCNGC 5 cut(s) 9, 12, 761, 892, 1221
GlaI GCGC 4 cut(s) 112, 764, 1508, 1949
GluI GCNGC 5 cut(s) 9, 12, 761, 892, 1221
GsuI CTGGAG 1 cut(s) 1349
HaeII RGCGCY 3 cut(s) 114, 766, 1510
HaeIII GGCC 2 cut(s) 1501, 1999
HapII CCGG 1 cut(s) 2063
HhaI GCGC 4 cut(s) 113, 765, 1509, 1950
Hin6I GCGC 4 cut(s) 111, 763, 1507, 1948
HinP1I GCGC 4 cut(s) 111, 763, 1507, 1948
HincII GTYRAC 2 cut(s) 253, 741
HindII GTYRAC 2 cut(s) 253, 741
HindIII AAGCTT 3 cut(s) 437, 704, 1450
HpaI GTTAAC 1 cut(s) 741
HpaII CCGG 1 cut(s) 2063
HphI GGTGA 2 cut(s) 425, 2045
Hpy166II GTNNAC 5 cut(s) 253, 279, 649, 741, 1083
Hpy8I GTNNAC 5 cut(s) 253, 279, 649, 741, 1083
HpyAV CCTTC 5 cut(s) 513, 1124, 1859, 1874, 2030
HpyCH4III ACNGT 6 cut(s) 166, 271, 454, 827, 1094, 1678
HpyCH4IV ACGT 1 cut(s) 129
HpyF10VI GCNNNNNNNGC 3 cut(s) 760, 857, 2129
HpyF3I CTNAG 6 cut(s) 560, 718, 1503, 1568, 1636, 2128
HpySE526I ACGT 1 cut(s) 129
HspAI GCGC 4 cut(s) 111, 763, 1507, 1948
Ksp22I TGATCA 3 cut(s) 673, 679, 1471
KspAI GTTAAC 1 cut(s) 741
Kzo9I GATC 8 cut(s) 367, 499, 673, 679, 1471, 1585, 1632, 1984
LmnI GCTCC 2 cut(s) 1392, 1545
Lsp1109I GCAGC 3 cut(s) 23, 878, 1232
LweI GCATC 3 cut(s) 481, 1232, 1410
MaeII ACGT 1 cut(s) 129
MaeIII GTNAC 6 cut(s) 356, 454, 827, 1345, 1712, 1736
MalI GATC 8 cut(s) 369, 501, 675, 681, 1473, 1587, 1634, 1986
MboI GATC 8 cut(s) 367, 499, 673, 679, 1471, 1585, 1632, 1984
MboII GAAGA 9 cut(s) 29, 88, 160, 698, 1239, 1305, 1541, 1819, 2003
MfeI CAATTG 1 cut(s) 466
MflI RGATCY 3 cut(s) 1585, 1632, 1984
MhlI GDGCHC 3 cut(s) 90, 1085, 1542
MluCI AATT 9 cut(s) 237, 466, 832, 914, 1016, 1114, 1575, 1619, 1895
MlyI GAGTC 2 cut(s) 1250, 1913
MmeI TCCRAC 2 cut(s) 1681, 1770
Mph1103I ATGCAT 2 cut(s) 1439, 1864
MroXI GAANNNNTTC 2 cut(s) 555, 1093
MseI TTAA 5 cut(s) 402, 740, 806, 1044, 1623
MslI CAYNNNNRTG 2 cut(s) 681, 1214
MspI CCGG 1 cut(s) 2063
MspR9I CCNGG 5 cut(s) 234, 411, 730, 1039, 2064
MunI CAATTG 1 cut(s) 466
Mva1269I GAATGC 5 cut(s) 213, 310, 1073, 1401, 1439
MvaI CCWGG 4 cut(s) 234, 411, 730, 1039
MwoI GCNNNNNNNGC 3 cut(s) 760, 857, 2129
NciI CCSGG 1 cut(s) 2064
NcoI CCATGG 1 cut(s) 596
NdeI CATATG 2 cut(s) 137, 853
NdeII GATC 8 cut(s) 367, 499, 673, 679, 1471, 1585, 1632, 1984
NlaIV GGNNCC 5 cut(s) 79, 535, 797, 1541, 1705
NmuCI GTSAC 3 cut(s) 356, 827, 1736
NsiI ATGCAT 2 cut(s) 1439, 1864
NspI RCATGY 6 cut(s) 1441, 1489, 1720, 1862, 2008, 2127
PaeI GCATGC 3 cut(s) 1441, 1489, 2127
PagI TCATGA 2 cut(s) 676, 682
PciI ACATGT 1 cut(s) 1716
PctI GAATGC 5 cut(s) 213, 310, 1073, 1401, 1439
PdmI GAANNNNTTC 2 cut(s) 555, 1093
PflMI CCANNNNNTGG 3 cut(s) 273, 602, 887
PkrI GCNGC 5 cut(s) 10, 13, 762, 893, 1222
PleI GAGTC 2 cut(s) 1249, 1913
PpsI GAGTC 2 cut(s) 1249, 1913
Ppu21I YACGTR 1 cut(s) 130
PscI ACATGT 1 cut(s) 1716
PshAI GACNNNNGTC 1 cut(s) 1644
PsiI TTATAA 1 cut(s) 161
Psp124BI GAGCTC 1 cut(s) 90
Psp6I CCWGG 4 cut(s) 232, 409, 728, 1037
PspGI CCWGG 4 cut(s) 232, 409, 728, 1037
PspN4I GGNNCC 5 cut(s) 79, 535, 797, 1541, 1705
PspPI GGNCC 5 cut(s) 276, 533, 593, 635, 1887
PstI CTGCAG 1 cut(s) 109
PsuI RGATCY 3 cut(s) 1585, 1632, 1984
RsaI GTAC 4 cut(s) 31, 143, 386, 1004
RsaNI GTAC 4 cut(s) 30, 142, 385, 1003
RseI CAYNNNNRTG 2 cut(s) 681, 1214
SacI GAGCTC 1 cut(s) 90
SaqAI TTAA 5 cut(s) 402, 740, 806, 1044, 1623
SatI GCNGC 5 cut(s) 9, 12, 761, 892, 1221
Sau3AI GATC 8 cut(s) 367, 499, 673, 679, 1471, 1585, 1632, 1984
Sau96I GGNCC 5 cut(s) 276, 533, 593, 635, 1887
SbfI CCTGCAGG 1 cut(s) 109
ScaI AGTACT 2 cut(s) 386, 1004
SchI GAGTC 2 cut(s) 1250, 1913
ScrFI CCNGG 5 cut(s) 234, 411, 730, 1039, 2064
SdaI CCTGCAGG 1 cut(s) 109
SduI GDGCHC 3 cut(s) 90, 1085, 1542
SfaNI GCATC 3 cut(s) 481, 1232, 1410
SfcI CTRYAG 3 cut(s) 105, 694, 1677
SinI GGWCC 5 cut(s) 276, 533, 593, 635, 1887
SmiMI CAYNNNNRTG 2 cut(s) 681, 1214
SmlI CTYRAG 4 cut(s) 507, 744, 1061, 2156
SmoI CTYRAG 4 cut(s) 507, 744, 1061, 2156
SnaBI TACGTA 1 cut(s) 130
SphI GCATGC 3 cut(s) 1441, 1489, 2127
Sse8387I CCTGCAGG 1 cut(s) 109
Sse9I AATT 9 cut(s) 237, 466, 832, 914, 1016, 1114, 1575, 1619, 1895
SsiI CCGC 2 cut(s) 9, 760
SspI AATATT 2 cut(s) 868, 1603
SstI GAGCTC 1 cut(s) 90
StyD4I CCNGG 5 cut(s) 232, 409, 728, 1037, 2062
StyI CCWWGG 2 cut(s) 596, 1390
TaaI ACNGT 6 cut(s) 166, 271, 454, 827, 1094, 1678
TaiI ACGT 1 cut(s) 132
TaqI TCGA 2 cut(s) 124, 1952
TasI AATT 9 cut(s) 237, 466, 832, 914, 1016, 1114, 1575, 1619, 1895
TatI WGTACW 3 cut(s) 29, 384, 1002
TauI GCSGC 2 cut(s) 11, 763
Tru1I TTAA 5 cut(s) 402, 740, 806, 1044, 1623
Tru9I TTAA 5 cut(s) 402, 740, 806, 1044, 1623
TscAI CASTG 7 cut(s) 205, 315, 361, 622, 1108, 1192, 2089
TseFI GTSAC 3 cut(s) 356, 827, 1736
TseI GCWGC 3 cut(s) 11, 891, 1220
Tsp45I GTSAC 3 cut(s) 356, 827, 1736
TspGWI ACGGA 1 cut(s) 1755
TspRI CASTG 7 cut(s) 205, 315, 361, 622, 1108, 1192, 2089
Van91I CCANNNNNTGG 3 cut(s) 273, 602, 887
VneI GTGCAC 1 cut(s) 1081
VpaK11BI GGWCC 5 cut(s) 276, 533, 593, 635, 1887
XagI CCTNNNNNAGG 2 cut(s) 507, 630
XapI RAATTY 5 cut(s) 237, 914, 1016, 1114, 1619
XbaI TCTAGA 2 cut(s) 1562, 2117
XceI RCATGY 6 cut(s) 1441, 1489, 1720, 1862, 2008, 2127
XmnI GAANNNNTTC 2 cut(s) 555, 1093
ZrmI AGTACT 2 cut(s) 386, 1004
Zsp2I ATGCAT 2 cut(s) 1439, 1864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.