MD05G1231700.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000484
Physical Location & Seq
Forward (+)
36486492 .. 36490037
3546 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1231700.v1.1.491

Sequence Viewer

Length: 2511 bp
ATGACAGACAAACATTCAAATCACATTACGAAAAATCCGTCAAAAGCTTCGGCTAAGATGTGCTTGCTTCTTTTCTACTCTTCTTTGCTGCTCATCGTCGCAGCAGTATTCTCCACAGCTGATGACGCCACAAGTACATTTCAGTCCATTAGAGACGGTGAGACTGTAGTTTCCACTGGCGGTACGTTTGAGCTAGGATTTTACAAGTATCCTGATGCTTCTAATAGACGGTACGTGGCGATATGGTACAAGCAGATATCTGTTACAACCATTGTATGGATTGCCAACAGAGACAAGCCCCTCGCTGATTTGTCCGGCGTTTTGAAGGTCACCAGCCCCGGAATTCTTGTCCTCAACCACAACATGAGCACAGTTTGGTCCTCCAACACATCGAGAACTGCACAGAATCCAGTGGCACGCCTTTTGGATTCGGGTAATCTTGTCGTGATAGATAGTAGTGATGATGACTCTGAGAACTTTCTGTGGCAAAGTTTTGATTACCCTGGCGATACATTCTTACCAGGAATGGAGATTGGTAGGAACACAGTTACAGGCTTCAATTGCCATCTTAGATCATGGAAAAGTCCTCAGGATCCTTCTCAAGGCAATTATACATATCAACTTGGTCCCAAAGGATATCAAGAAATATTTGTGAGCAACGGTTCGGTCATAAAATTTCGGACTGGACCATGGAATGGAGTCCGGTTCAGTGGAACGCCTCATTTAAATCCAAACCCAATATACACATACGGTTTTGTTTCTGAGCCGGATAAAATGTACTACAGTTACAAGCTTCACAACAGCTCAATCTTTTCGAGGGTGGTGTTAACTTCAGATGGACTTGTGCAGCGCTACACATGGATTGATAGAACCAAAGATTGGTTTCTTTACCTAACAGCCCAGATTGATAACTGTGACAACTATGCATTCTGTGGTGTATATGGTGCATGTAACATTGAAAAGTCCCCGGTTTGTAGCTGTTTGAAAGGATTTACACCAAAGTTTCCGAAAGAATGGGATTTGGTAGATTGGTCTGATGGTTGTGTGAGAAAGACTTCTCTAAATTGCACCGGAGACGTGTTCCAAAAGTACTCGGGTTTGAAATTGCCTAGCACAGAACAATCCTGGTTTAACAGAAGTATGAACCTCAAGGAATGCCAGATGGTGTGCATGAAGAACTGCTCCTGCACGGCTTATACAAATTTGGATATTCGGGATGGAGGAACTGGGTGCTTGCTGTGGTACGGCGGTCTGATTGATATAAGATACTATTCCGAAAACGGGCAAGATATTTATATAAGAATGGCCGCAGCAGAACTAGATTATGAAGATGATGCAAAGATCAACGCTAAATACTCTGAATCCAATGCAAAGAAAATGAAAATCATAATAAGCACTACTGTGTTGTCTACCGGACTGCTGCTCTTGGGCCTTGCCCTGCTGTTTTTTGTTTGGAAGAAGCAGCACCAAAAAGTTGGAAAACTGGGACACAACCAAAAGGAGGATTTGGAATTACCGTTATTTGACTTGATGACTATAGTTTCTGCAACCGGTAACTTTTCAATTGAAAACAAACTTGGTGAAGGCGGTTTTGGATCTGTCTTTAAGGGTACGATGGAAGATGGACAAGAAATCGCTGTGAAAAGGCTTTCAAAAAGTTCTAGACAAGGACTCGACGAGTTCAAGAATGAAGTTACACATATTGCCAAACTTCAACATAGGAATCTAGTGAAGCTTCTTGGATGCTGCATTCAAGAAGACGAGATGATACTGATCTACGAGTTCATGCCTAACAAGAGCTTAGACTTTTTTATTTTCGATCAAAGAAGAAGGATGTTATTAGACTGGCCGAAGCGCTTTGAAATTATTAATGGGATAGCTCGAGGGCTCCTCTATCTTCATCAAGATTCTAGATTGAGAGTTATTCATAGAGATCTCAAAGCAAGTAACATTTTATTAGGCCGTGAATTTAACCCGAAAATCTCAGACTTTGGCTTGGCTAGAAGCTTCGGAGGAAATGAAACGAAAGCAGAGACGAAGAAAGTGGTTGGAACATACGGTTACATGTCCCCAGAATATGCAATTGATGGTTTCTACTCTATAAAGTCCGACGTCTATAGCTTTGGTGTTCTGGTGCTAGAGATAGTGAGTGGGAGCAAAAATAGAGGATTCTCTCATCCAGACCACAAACTCAACCTTCTTGGACATGCATGGATGCTACACACAGAAGGCAGGCCTCTCGAACTACTTGATACATCGGTAGAGGACTCCATCACTCTGTTTGAAGTTGTAAGAACAATTCATGTGGGTCTTTTGTGCGTGCAGCGGAATCCAGAGGACAGGCCGAGCATGTCAGCTGCAGTTCTAATGTTGAGTGGTGAAGGTGCATTGCCTCAACCTTTGAAACCTGGGTTTTATAGTGAAAGGGATTTGACTGAACTCGAAGCCAGTCATTCTTCTAAAGCATGCTCAGCTAATGAAGTCACTATTTCAGTACTTGAGGCTCGATAA

Protein Analysis

837

Amino Acids

93.99

Weight (kDa)

6.81

Isoelectric Point (pI)

41.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 90 - 194 1.9e-36 D-mannose binding lectin
S_locus_glycop PF00954 226 - 335 2.4e-32 S-locus glycoprotein domain
PAN_2 PF08276 356 - 421 3.5e-19 PAN-like domain
PK_Tyr_Ser-Thr PF07714 521 - 786 1.3e-47 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 521 - 785 7.7e-45 Protein kinase domain
DUF3403 PF11883 794 - 836 2.8e-08 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 2115
AccB7I CCANNNNNTGG 3 cut(s) 276, 695, 879
AccI GTMKAC 1 cut(s) 1409
AciI CCGC 5 cut(s) 180, 1248, 1308, 1587, 2326
AclWI GGATC 3 cut(s) 587, 600, 1603
AcoI YGGCCR 2 cut(s) 1305, 1847
AcsI RAATTY 4 cut(s) 342, 674, 1201, 1967
AcuI CTGAAG 1 cut(s) 816
AcyI GRCGYC 2 cut(s) 126, 2112
AfaI GTAC 9 cut(s) 136, 184, 233, 248, 779, 1091, 1244, 1612, 2496
AfeI AGCGCT 2 cut(s) 851, 1856
AfiI CCNNNNNNNGG 6 cut(s) 276, 602, 695, 879, 1281, 1501
AflIII ACRYGT 2 cut(s) 1077, 2064
AgeI ACCGGT 1 cut(s) 1550
AjiI CACGTC 1 cut(s) 1078
AjnI CCWGG 4 cut(s) 502, 520, 1124, 2407
AjuI GAANNNNNNNTTGG 6 cut(s) 862, 894, 1560, 1575, 1592, 1607
AleI CACNNNNGTG 1 cut(s) 1400
Alw21I GWGCWC 1 cut(s) 371
Alw26I GTCTC 5 cut(s) 147, 155, 285, 1068, 2027
AlwI GGATC 3 cut(s) 587, 600, 1603
Ama87I CYCGRG 2 cut(s) 1093, 1881
Aor51HI AGCGCT 2 cut(s) 851, 1856
AoxI GGCC 6 cut(s) 1305, 1429, 1847, 1960, 2234, 2342
ApeKI GCWGC 9 cut(s) 88, 101, 847, 1310, 1420, 1462, 1746, 2323, 2357
ApoI RAATTY 4 cut(s) 342, 674, 1201, 1967
ArsI GACNNNNNNTTYG 2 cut(s) 23, 55
AseI ATTAAT 1 cut(s) 1869
AsiGI ACCGGT 1 cut(s) 1550
Asp700I GAANNNNTTC 1 cut(s) 1054
AspLEI GCGC 2 cut(s) 852, 1857
AspS9I GGNCC 4 cut(s) 378, 626, 686, 1429
AsuC2I CCSGG 2 cut(s) 339, 968
AsuHPI GGTGA 4 cut(s) 170, 322, 1592, 2390
AvaI CYCGRG 2 cut(s) 1093, 1881
AvaII GGWCC 3 cut(s) 378, 626, 686
AxyI CCTNAGG 1 cut(s) 588
BaeI ACNNNNGTAYC 4 cut(s) 1234, 1267, 2244, 2277
BamHI GGATCC 1 cut(s) 592
BanII GRGCYC 1 cut(s) 1890
BbsI GAAGAC 1 cut(s) 1764
Bbv12I GWGCWC 1 cut(s) 371
BbvI GCAGC 9 cut(s) 75, 113, 859, 1322, 1407, 1474, 1733, 2335, 2344
BccI CCATC 9 cut(s) 573, 830, 1031, 1156, 1211, 1609, 1616, 2081, 2279
BceAI ACGGC 3 cut(s) 1206, 1261, 1947
BcgI CGANNNNNNTGC 2 cut(s) 2221, 2255
BciT130I CCWGG 4 cut(s) 504, 522, 1126, 2409
BciVI GTATCC 1 cut(s) 219
BcnI CCSGG 2 cut(s) 339, 968
BcoDI GTCTC 5 cut(s) 147, 155, 285, 1068, 2027
BfaI CTAG 8 cut(s) 194, 1110, 1319, 1662, 1727, 1911, 2001, 2138
BfmI CTRYAG 5 cut(s) 165, 781, 1536, 2116, 2358
BfoI RGCGCY 2 cut(s) 853, 1858
BfuI GTATCC 1 cut(s) 219
BglII AGATCT 1 cut(s) 1933
BlpI GCTNAGC 1 cut(s) 2470
BmcAI AGTACT 2 cut(s) 1091, 2496
Bme1390I CCNGG 6 cut(s) 339, 504, 522, 968, 1126, 2409
Bme18I GGWCC 3 cut(s) 378, 626, 686
BmeT110I CYCGRG 2 cut(s) 1093, 1881
BmgBI CACGTC 1 cut(s) 1078
BmgT120I GGNCC 4 cut(s) 378, 626, 686, 1429
BmiI GGNNCC 3 cut(s) 594, 628, 1889
BmrFI CCNGG 6 cut(s) 339, 504, 522, 968, 1126, 2409
BmrI ACTGGG 2 cut(s) 1236, 1493
BmsI GCATC 4 cut(s) 205, 1324, 1733, 2205
BmuI ACTGGG 2 cut(s) 1236, 1493
BpiI GAAGAC 1 cut(s) 1764
BplI GAGNNNNNCTC 2 cut(s) 1875, 1907
Bpu1102I GCTNAGC 1 cut(s) 2470
BpuEI CTTGAG 2 cut(s) 585, 1133
BpuMI CCSGG 2 cut(s) 339, 968
BsaAI YACGTR 1 cut(s) 235
BsaBI GATNNNNATC 1 cut(s) 1772
BsaHI GRCGYC 2 cut(s) 126, 2112
BsaJI CCNNGG 5 cut(s) 337, 502, 689, 966, 2408
BsaWI WCCGGW 4 cut(s) 702, 1070, 1412, 1550
Bsc4I CCNNNNNNNGG 6 cut(s) 276, 602, 695, 879, 1281, 1501
Bse118I RCCGGY 1 cut(s) 1550
Bse1I ACTGG 7 cut(s) 181, 410, 688, 1231, 1488, 1850, 2448
Bse21I CCTNAGG 1 cut(s) 588
Bse3DI GCAATG 1 cut(s) 2387
Bse8I GATNNNNATC 1 cut(s) 1772
BseBI CCWGG 4 cut(s) 504, 522, 1126, 2409
BseDI CCNNGG 5 cut(s) 337, 502, 689, 966, 2408
BseGI GGATG 5 cut(s) 1222, 1748, 1839, 2176, 2220
BseJI GATNNNNATC 1 cut(s) 1772
BseLI CCNNNNNNNGG 6 cut(s) 276, 602, 695, 879, 1281, 1501
BseMI GCAATG 1 cut(s) 2387
BseMII CTCAG 5 cut(s) 462, 602, 753, 1998, 2484
BseNI ACTGG 7 cut(s) 181, 410, 688, 1231, 1488, 1850, 2448
BseRI GAGGAG 1 cut(s) 1880
BseXI GCAGC 9 cut(s) 75, 113, 859, 1322, 1407, 1474, 1733, 2335, 2344
BsgI GTGCAG 4 cut(s) 384, 866, 1171, 2342
BshFI GGCC 6 cut(s) 1307, 1431, 1849, 1962, 2236, 2344
BshTI ACCGGT 1 cut(s) 1550
BsiHKAI GWGCWC 1 cut(s) 371
BsiHKCI CYCGRG 2 cut(s) 1093, 1881
BsiSI CCGG 8 cut(s) 315, 339, 703, 767, 968, 1071, 1413, 1551
BslFI GGGAC 4 cut(s) 612, 949, 1500, 2053
BslI CCNNNNNNNGG 6 cut(s) 276, 602, 695, 879, 1281, 1501
BsmAI GTCTC 5 cut(s) 147, 155, 285, 1068, 2027
BsmBI CGTCTC 3 cut(s) 147, 1068, 2027
BsmFI GGGAC 4 cut(s) 612, 949, 1500, 2053
BsmI GAATGC 3 cut(s) 926, 1160, 1749
BsnI GGCC 6 cut(s) 1307, 1431, 1849, 1962, 2236, 2344
BsoBI CYCGRG 2 cut(s) 1093, 1881
Bsp1286I GDGCHC 2 cut(s) 371, 1890
Bsp143I GATC 7 cut(s) 572, 592, 1341, 1595, 1773, 1819, 1933
Bsp1720I GCTNAGC 1 cut(s) 2470
Bsp19I CCATGG 1 cut(s) 689
BspACI CCGC 5 cut(s) 180, 1248, 1308, 1587, 2326
BspANI GGCC 6 cut(s) 1307, 1431, 1849, 1962, 2236, 2344
BspCNI CTCAG 5 cut(s) 463, 601, 754, 1997, 2483
BspLI GGNNCC 3 cut(s) 594, 628, 1889
BspMAI CTGCAG 1 cut(s) 2362
BspPI GGATC 3 cut(s) 587, 600, 1603
BsrDI GCAATG 1 cut(s) 2387
BsrFI RCCGGY 1 cut(s) 1550
BsrI ACTGG 7 cut(s) 181, 410, 688, 1231, 1488, 1850, 2448
BssAI RCCGGY 1 cut(s) 1550
BssECI CCNNGG 5 cut(s) 337, 502, 689, 966, 2408
BssMI GATC 7 cut(s) 572, 592, 1341, 1595, 1773, 1819, 1933
BssNI GRCGYC 2 cut(s) 126, 2112
BssT1I CCWWGG 1 cut(s) 689
Bst2UI CCWGG 4 cut(s) 504, 522, 1126, 2409
Bst6I CTCTTC 1 cut(s) 85
BstACI GRCGYC 2 cut(s) 126, 2112
BstBAI YACGTR 1 cut(s) 235
BstC8I GCNNGC 6 cut(s) 65, 418, 1235, 2234, 2321, 2467
BstDEI CTNAG 8 cut(s) 54, 471, 569, 588, 762, 1801, 1984, 2470
BstDSI CCRYGG 1 cut(s) 689
BstEII GGTNACC 1 cut(s) 328
BstENI CCTNNNNNAGG 1 cut(s) 600
BstF5I GGATG 5 cut(s) 1222, 1748, 1839, 2176, 2220
BstH2I RGCGCY 2 cut(s) 853, 1858
BstHHI GCGC 2 cut(s) 852, 1857
BstKTI GATC 7 cut(s) 575, 595, 1344, 1598, 1776, 1822, 1936
BstMAI GTCTC 5 cut(s) 147, 155, 285, 1068, 2027
BstMBI GATC 7 cut(s) 572, 592, 1341, 1595, 1773, 1819, 1933
BstMWI GCNNNNNNNGC 3 cut(s) 125, 561, 2471
BstNI CCWGG 4 cut(s) 504, 522, 1126, 2409
BstNSI RCATGY 5 cut(s) 951, 2068, 2210, 2353, 2469
BstPI GGTNACC 1 cut(s) 328
BstSCI CCNGG 6 cut(s) 337, 502, 520, 966, 1124, 2407
BstSFI CTRYAG 5 cut(s) 165, 781, 1536, 2116, 2358
BstV1I GCAGC 9 cut(s) 75, 113, 859, 1322, 1407, 1474, 1733, 2335, 2344
BstV2I GAAGAC 1 cut(s) 1764
BstX2I RGATCY 3 cut(s) 592, 1595, 1933
BstXI CCANNNNNNTGG 1 cut(s) 1475
BstYI RGATCY 3 cut(s) 592, 1595, 1933
Bsu36I CCTNAGG 1 cut(s) 588
BsuI GTATCC 1 cut(s) 219
BsuRI GGCC 6 cut(s) 1307, 1431, 1849, 1962, 2236, 2344
BtgI CCRYGG 1 cut(s) 689
BtrI CACGTC 1 cut(s) 1078
BtsCI GGATG 5 cut(s) 1222, 1748, 1839, 2176, 2220
BtsIMutI CAGTG 3 cut(s) 174, 417, 715
Cac8I GCNNGC 6 cut(s) 65, 418, 1235, 2234, 2321, 2467
CfoI GCGC 2 cut(s) 852, 1857
Cfr10I RCCGGY 1 cut(s) 1550
Cfr13I GGNCC 4 cut(s) 378, 626, 686, 1429
CseI GACGC 1 cut(s) 134
Csp6I GTAC 9 cut(s) 135, 183, 232, 247, 778, 1090, 1243, 1611, 2495
CspAI ACCGGT 1 cut(s) 1550
CspCI CAANNNNNGTGG 2 cut(s) 2286, 2321
CviQI GTAC 9 cut(s) 135, 183, 232, 247, 778, 1090, 1243, 1611, 2495
DdeI CTNAG 8 cut(s) 54, 471, 569, 588, 762, 1801, 1984, 2470
DpnI GATC 7 cut(s) 574, 594, 1343, 1597, 1775, 1821, 1935
DpnII GATC 7 cut(s) 572, 592, 1341, 1595, 1773, 1819, 1933
DraI TTTAAA 1 cut(s) 726
EaeI YGGCCR 2 cut(s) 1305, 1847
Eam1104I CTCTTC 1 cut(s) 85
EarI CTCTTC 1 cut(s) 85
Eco130I CCWWGG 1 cut(s) 689
Eco147I AGGCCT 1 cut(s) 2236
Eco24I GRGCYC 1 cut(s) 1890
Eco32I GATATC 2 cut(s) 258, 638
Eco47I GGWCC 3 cut(s) 378, 626, 686
Eco47III AGCGCT 2 cut(s) 851, 1856
Eco57I CTGAAG 1 cut(s) 816
Eco81I CCTNAGG 1 cut(s) 588
Eco88I CYCGRG 2 cut(s) 1093, 1881
Eco91I GGTNACC 1 cut(s) 328
EcoNI CCTNNNNNAGG 1 cut(s) 600
EcoO65I GGTNACC 1 cut(s) 328
EcoRI GAATTC 1 cut(s) 342
EcoRII CCWGG 4 cut(s) 502, 520, 1124, 2407
EcoRV GATATC 2 cut(s) 258, 638
EcoT14I CCWWGG 1 cut(s) 689
EcoT22I ATGCAT 2 cut(s) 928, 2212
EcoT38I GRGCYC 1 cut(s) 1890
ErhI CCWWGG 1 cut(s) 689
Esp3I CGTCTC 3 cut(s) 147, 1068, 2027
FalI AAGNNNNNCTT 2 cut(s) 47, 79
FaqI GGGAC 4 cut(s) 612, 949, 1500, 2053
FblI GTMKAC 1 cut(s) 1409
FokI GGATG 5 cut(s) 1229, 1755, 1846, 2163, 2227
FriOI GRGCYC 1 cut(s) 1890
FspBI CTAG 8 cut(s) 194, 1110, 1319, 1662, 1727, 1911, 2001, 2138
GlaI GCGC 2 cut(s) 851, 1856
HaeII RGCGCY 2 cut(s) 853, 1858
HaeIII GGCC 6 cut(s) 1307, 1431, 1849, 1962, 2236, 2344
HapII CCGG 8 cut(s) 315, 339, 703, 767, 968, 1071, 1413, 1551
HgaI GACGC 1 cut(s) 134
HhaI GCGC 2 cut(s) 852, 1857
Hin1I GRCGYC 2 cut(s) 126, 2112
Hin6I GCGC 2 cut(s) 850, 1855
HinP1I GCGC 2 cut(s) 850, 1855
HincII GTYRAC 1 cut(s) 828
HindII GTYRAC 1 cut(s) 828
HindIII AAGCTT 4 cut(s) 45, 791, 1733, 2005
HpaI GTTAAC 1 cut(s) 828
HpaII CCGG 8 cut(s) 315, 339, 703, 767, 968, 1071, 1413, 1551
HphI GGTGA 4 cut(s) 170, 322, 1592, 2390
Hpy166II GTNNAC 2 cut(s) 828, 1410
Hpy8I GTNNAC 2 cut(s) 828, 1410
Hpy99I CGWCG 3 cut(s) 101, 1679, 2114
HpyAV CCTTC 7 cut(s) 319, 606, 1577, 1824, 2207, 2222, 2375
HpyCH4IV ACGT 4 cut(s) 185, 234, 1077, 2112
HpyF10VI GCNNNNNNNGC 3 cut(s) 125, 561, 2471
HpyF3I CTNAG 8 cut(s) 54, 471, 569, 588, 762, 1801, 1984, 2470
HpySE526I ACGT 4 cut(s) 185, 234, 1077, 2112
Hsp92I GRCGYC 2 cut(s) 126, 2112
HspAI GCGC 2 cut(s) 850, 1855
KspAI GTTAAC 1 cut(s) 828
Kzo9I GATC 7 cut(s) 572, 592, 1341, 1595, 1773, 1819, 1933
LmnI GCTCC 3 cut(s) 1187, 1893, 2154
Lsp1109I GCAGC 9 cut(s) 75, 113, 859, 1322, 1407, 1474, 1733, 2335, 2344
LweI GCATC 4 cut(s) 205, 1324, 1733, 2205
MaeI CTAG 8 cut(s) 194, 1110, 1319, 1662, 1727, 1911, 2001, 2138
MaeII ACGT 4 cut(s) 185, 234, 1077, 2112
MalI GATC 7 cut(s) 574, 594, 1343, 1597, 1775, 1821, 1935
MboI GATC 7 cut(s) 572, 592, 1341, 1595, 1773, 1819, 1933
MfeI CAATTG 3 cut(s) 559, 1563, 2082
MflI RGATCY 3 cut(s) 592, 1595, 1933
MhlI GDGCHC 2 cut(s) 371, 1890
MlyI GAGTC 4 cut(s) 461, 708, 1665, 2261
MmeI TCCRAC 4 cut(s) 408, 1456, 2029, 2133
Mph1103I ATGCAT 2 cut(s) 928, 2212
MroXI GAANNNNTTC 1 cut(s) 1054
MseI TTAA 6 cut(s) 725, 827, 1131, 1605, 1869, 1971
MslI CAYNNNNRTG 1 cut(s) 1400
MspA1I CMGCKG 3 cut(s) 119, 2326, 2357
MspI CCGG 8 cut(s) 315, 339, 703, 767, 968, 1071, 1413, 1551
MspR9I CCNGG 6 cut(s) 339, 504, 522, 968, 1126, 2409
MunI CAATTG 3 cut(s) 559, 1563, 2082
Mva1269I GAATGC 3 cut(s) 926, 1160, 1749
MvaI CCWGG 4 cut(s) 504, 522, 1126, 2409
MwoI GCNNNNNNNGC 3 cut(s) 125, 561, 2471
NciI CCSGG 2 cut(s) 339, 968
NcoI CCATGG 1 cut(s) 689
NdeII GATC 7 cut(s) 572, 592, 1341, 1595, 1773, 1819, 1933
NlaIV GGNNCC 3 cut(s) 594, 628, 1889
NmeAIII GCCGAG 1 cut(s) 2370
NmuCI GTSAC 3 cut(s) 328, 914, 2482
NsiI ATGCAT 2 cut(s) 928, 2212
NspI RCATGY 5 cut(s) 951, 2068, 2210, 2353, 2469
OliI CACNNNNGTG 1 cut(s) 1400
PaeI GCATGC 1 cut(s) 2469
PaeR7I CTCGAG 1 cut(s) 1881
PceI AGGCCT 1 cut(s) 2236
PciI ACATGT 1 cut(s) 2064
PcsI WCGNNNNNNNCGW 1 cut(s) 35
PctI GAATGC 3 cut(s) 926, 1160, 1749
PdmI GAANNNNTTC 1 cut(s) 1054
PfeI GAWTC 7 cut(s) 406, 428, 1361, 1723, 1907, 2169, 2329
PflMI CCANNNNNTGG 3 cut(s) 276, 695, 879
PinAI ACCGGT 1 cut(s) 1550
PleI GAGTC 4 cut(s) 461, 707, 1665, 2261
PpsI GAGTC 4 cut(s) 461, 707, 1665, 2261
Ppu21I YACGTR 1 cut(s) 235
PscI ACATGT 1 cut(s) 2064
PshBI ATTAAT 1 cut(s) 1869
Psp6I CCWGG 4 cut(s) 502, 520, 1124, 2407
PspEI GGTNACC 1 cut(s) 328
PspGI CCWGG 4 cut(s) 502, 520, 1124, 2407
PspN4I GGNNCC 3 cut(s) 594, 628, 1889
PspPI GGNCC 4 cut(s) 378, 626, 686, 1429
PspXI VCTCGAGB 1 cut(s) 1881
PstI CTGCAG 1 cut(s) 2362
PsuI RGATCY 3 cut(s) 592, 1595, 1933
PvuII CAGCTG 2 cut(s) 119, 2357
RsaI GTAC 9 cut(s) 136, 184, 233, 248, 779, 1091, 1244, 1612, 2496
RsaNI GTAC 9 cut(s) 135, 183, 232, 247, 778, 1090, 1243, 1611, 2495
RseI CAYNNNNRTG 1 cut(s) 1400
SaqAI TTAA 6 cut(s) 725, 827, 1131, 1605, 1869, 1971
Sau3AI GATC 7 cut(s) 572, 592, 1341, 1595, 1773, 1819, 1933
Sau96I GGNCC 4 cut(s) 378, 626, 686, 1429
ScaI AGTACT 2 cut(s) 1091, 2496
SchI GAGTC 4 cut(s) 461, 708, 1665, 2261
ScrFI CCNGG 6 cut(s) 339, 504, 522, 968, 1126, 2409
SduI GDGCHC 2 cut(s) 371, 1890
SfaNI GCATC 4 cut(s) 205, 1324, 1733, 2205
SfcI CTRYAG 5 cut(s) 165, 781, 1536, 2116, 2358
Sfr274I CTCGAG 1 cut(s) 1881
SinI GGWCC 3 cut(s) 378, 626, 686
SlaI CTCGAG 1 cut(s) 1881
SmiI ATTTAAAT 1 cut(s) 726
SmiMI CAYNNNNRTG 1 cut(s) 1400
SmlI CTYRAG 4 cut(s) 600, 1148, 1881, 2498
SmoI CTYRAG 4 cut(s) 600, 1148, 1881, 2498
SphI GCATGC 1 cut(s) 2469
SseBI AGGCCT 1 cut(s) 2236
SsiI CCGC 5 cut(s) 180, 1248, 1308, 1587, 2326
SspI AATATT 1 cut(s) 648
SspMI CTAG 8 cut(s) 194, 1110, 1319, 1662, 1727, 1911, 2001, 2138
StuI AGGCCT 1 cut(s) 2236
StyD4I CCNGG 6 cut(s) 337, 502, 520, 966, 1124, 2407
StyI CCWWGG 1 cut(s) 689
SwaI ATTTAAAT 1 cut(s) 726
TaiI ACGT 4 cut(s) 188, 237, 1080, 2115
TaqI TCGA 8 cut(s) 392, 815, 1674, 1818, 1882, 2241, 2442, 2506
TaqII GACCGA 1 cut(s) 655
TatI WGTACW 4 cut(s) 134, 777, 1089, 2494
TauI GCSGC 1 cut(s) 1310
TfiI GAWTC 7 cut(s) 406, 428, 1361, 1723, 1907, 2169, 2329
Tru1I TTAA 6 cut(s) 725, 827, 1131, 1605, 1869, 1971
Tru9I TTAA 6 cut(s) 725, 827, 1131, 1605, 1869, 1971
TscAI CASTG 3 cut(s) 181, 417, 715
TseFI GTSAC 3 cut(s) 328, 914, 2482
TseI GCWGC 9 cut(s) 88, 101, 847, 1310, 1420, 1462, 1746, 2323, 2357
Tsp45I GTSAC 3 cut(s) 328, 914, 2482
TspGWI ACGGA 1 cut(s) 27
TspRI CASTG 3 cut(s) 181, 417, 715
Van91I CCANNNNNTGG 3 cut(s) 276, 695, 879
VpaK11BI GGWCC 3 cut(s) 378, 626, 686
VspI ATTAAT 1 cut(s) 1869
XagI CCTNNNNNAGG 1 cut(s) 600
XapI RAATTY 4 cut(s) 342, 674, 1201, 1967
XbaI TCTAGA 2 cut(s) 1661, 1910
XceI RCATGY 5 cut(s) 951, 2068, 2210, 2353, 2469
XhoI CTCGAG 1 cut(s) 1881
XmiI GTMKAC 1 cut(s) 1409
XmnI GAANNNNTTC 1 cut(s) 1054
XspI CTAG 8 cut(s) 194, 1110, 1319, 1662, 1727, 1911, 2001, 2138
ZraI GACGTC 1 cut(s) 2113
ZrmI AGTACT 2 cut(s) 1091, 2496
Zsp2I ATGCAT 2 cut(s) 928, 2212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.