RLG00000032927

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000484
Physical Location & Seq
Forward (+)
21010119 .. 21015054
4936 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032927

Sequence Viewer

Length: 2427 bp
ATGAAAGGGATAGAGAAAAGGATGGGAATTGACTTCAAGAATAATGCCAAAGAGTACCCAAATCAGAAACCTGCAGTTTTGTTAGAGTTGAGGGATTTGGATTACAAGATTTGTCCTGTGGAGTTTGGAGTACGCATTCCTAATTGCCATCCGAAGTTGAATGGCATTGGAACAAGGGACTGGAGGGATAATGAGTCTTCCATGGAAATGAGGTGTTCGAGTATTACAGTCATGAAAAATCCATTGAAGGTTTTAATTAGGATGTGCTTTCATCCTATTTGCTCTTTTCTGCTCATCATTACAGCGATATTTTCCACAGTGGGAGGCAGCACAAGTACATTTCAGTCCATTAGAGATGGCCAGACTATAGTTTCAACTACTGGAAACTTTGAGCTTGGATTTTTTAGTCCTGCTACTGGTTCTAATAATCGATACCTTGGGATATGGTACAAGAAGATATCGGCTACCATAGTGTGGGTTGCCAACAGAGAAGCACCCCTTACTGATTCATCAGGTGTTCTGCAGGTCACCGACCCTGGAATTCTTGTCCTCCTCAACCATAACAAGAGCATAGTTTGGTCCTCAAACACATCAAGATCTGCAGAGAATCTAGTGGCCCAGCTTTTGGATTCAGGAAATCTTGTTGTAACAGACGGCAGTGATACAGACCCTGAGAACTTCTTGTGGCAAAGTTTTGATTACCCAAGTGATACACTCCTACCAGGTATGAAGCTTGGTTGGAACAAAGTTACTGGCTTCAGTAGGCATCTTACATCATGGAGCAGTCCACAGGATCCTTCTCTAGGAAAATATACATATGAACTTGATCGCAGAGGATATGGAGAAGAAATTTTGAAGAAGGATTCAGTTATGATATGGCGAACTGGACCATGGAATGGAATCCGATTCAGTGGCACGCCTCATTTAACTCCAAACTCCATATTCACATACAATCTTGTTTTTGATGATCATGATGAAGAATATTATATTTTTGAGCTTCTTAACAGCTCAGTTTATTCAAGATTGATCCTCACTCCAGATGGGCGTGTTCAGCGCTACACTTGGATTGATACAACCCAAACTTGGGGATTTCTTTATCAAACAGCTTTAATTGATAACTGCGACAGTTATGCATTCTGTGGTCCAAATGGTGCATGCAGTATTGACAGTTCCCCAGTATGTCGCTGCTTGAATGGATTTCAACCCAAAAATCCAAAGGACTGGGATGTAGTGGATTGGTCAAATGGCTGTGTGAGAAGGACTCCACTAAATTCAGTAAATTGCAGCGGTGACTTGTTTGAGAAGTACTCTGCAGTGAAATTGCCAAACACAGAGCAGTCCTGGTTCAACAGAAGTATGAATCTGAAGGAATGTGAAATAGTGTGCTGGAAGAACTGTTCCTGCACAGCTTATGCAAATTTGGATATCACGGATGGAGGATCTGGCTGCTTGCTGTGGTTTGGCAACCTAATTGATATTAGAAATTTAACTGTAAATGGGCAAGATATTTATATAAGAATGGCTGCATCAGAACTAGATGGACAGGAAATAGCTGTGAAGAGGCTCTCGAAATATTCCACTCAAGGACTGGATGAGTTCAAGAATGAGGTTACACATATTGTCAAACTTCAGCACAGGAATCTAGTGAGGCTTCTAGGATGCTGCATTCAGGAAGATGAAATGATGCTGATCTACGAATGCATGCCTAACAAAAGCTTGGACTTCTTTATTTTTGATCAAAGAAGAAGCTTCTTACTAGATTGGTCTAAGCGCTTTGACATTATTAATGGTATTGCTCGGGGGATCCTTTATCTTCATCAAGATTCTAGACTGAGGGTAATTCATAGAGATCTCAAAGCAGGCAATATCTTATTAGACAGTGAATTCAACCCAAAAATCTCAGACTTCGGCCTGGCTAGAAGTTTTGGAGGAAATGAAACTAAAGCAGAAACGAATAAAGTGGTCGGGACATATGGTTACATGTCCCCAGAATATGCAATTGACGGGTTCTACTCGATCAAATCTGACGTATATAGCTTTGGTGTTTTGATGCTGGAGATAATAAGTGGGAAGAGAAACAGAGGATTCCTTCATCCAGGCCACAACCTCAACCTGCTTGGACATGCATGGAACTTACACACAGAAGGCAGGTCCATTGAATTGCTTGCTACATCAGTAGGGGACTCCATTAATCTACAAGAAGTTCTGCGAGCGATTCATGTGGGTCTTTTATGTGTTCAGCAAAATCCACAAGACAGGCCAAGCATGTCAGCTGCAGTTCTAATGTTAAGTGGTGAAGGTGTGCTGCCTGTACCTCAGAAACCTGGTTTTTATAATGAGAGGGCTCTAACTGAACTCCAAGTCCATTCTTCTTCACAAGCATGTTCAGTTAATATGCTCACTTCCACCCTATTCGAGCCTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

809

Amino Acids

91.08

Weight (kDa)

6.1

Isoelectric Point (pI)

43.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 156 - 260 3.4e-40 D-mannose binding lectin
S_locus_glycop PF00954 293 - 404 2.5e-29 S-locus glycoprotein domain
PAN_2 PF08276 428 - 493 1.1e-19 PAN-like domain
Pkinase PF00069 508 - 692 1.7e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 510 - 758 1.4e-40 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2334
Acc36I ACCTGC 4 cut(s) 79, 514, 2121, 2139
AccB7I CCANNNNNTGG 3 cut(s) 474, 625, 896
AciI CCGC 1 cut(s) 1287
AclWI GGATC 6 cut(s) 788, 801, 1021, 1447, 1798, 1811
AcoI YGGCCR 1 cut(s) 358
AcsI RAATTY 6 cut(s) 540, 849, 1270, 1417, 1483, 1883
AcuI CTGAAG 3 cut(s) 742, 1385, 1613
AfaI GTAC 6 cut(s) 56, 132, 337, 449, 1307, 2313
AfeI AGCGCT 2 cut(s) 1055, 1772
AfiI CCNNNNNNNGG 7 cut(s) 416, 474, 625, 803, 896, 1083, 1084
AflIII ACRYGT 1 cut(s) 1980
AjnI CCWGG 6 cut(s) 535, 721, 1340, 1911, 2095, 2323
AloI GAACNNNNNNTCC 4 cut(s) 1381, 1413, 2346, 2378
AlwI GGATC 6 cut(s) 788, 801, 1021, 1447, 1798, 1811
AlwNI CAGNNNCTG 1 cut(s) 671
Ama87I CYCGRG 1 cut(s) 1797
Aor51HI AGCGCT 2 cut(s) 1055, 1772
AoxI GGCC 5 cut(s) 358, 615, 1909, 2098, 2258
ApeKI GCWGC 8 cut(s) 327, 1185, 1284, 1446, 1523, 1662, 2273, 2305
ApoI RAATTY 6 cut(s) 540, 849, 1270, 1417, 1483, 1883
AseI ATTAAT 2 cut(s) 1785, 2190
AspLEI GCGC 2 cut(s) 1056, 1773
AspS9I GGNCC 5 cut(s) 579, 616, 887, 1142, 2151
AsuHPI GGTGA 3 cut(s) 520, 1301, 2306
AvaI CYCGRG 1 cut(s) 1797
AvaII GGWCC 4 cut(s) 579, 887, 1142, 2151
BalI TGGCCA 1 cut(s) 360
BamHI GGATCC 2 cut(s) 793, 1803
BanII GRGCYC 1 cut(s) 2347
BbsI GAAGAC 1 cut(s) 189
BbvI GCAGC 8 cut(s) 339, 1172, 1296, 1433, 1510, 1649, 2260, 2292
BccI CCATC 6 cut(s) 16, 156, 350, 1034, 1427, 1532
BceAI ACGGC 1 cut(s) 670
BcgI CGANNNNNNTGC 2 cut(s) 1112, 1146
BciT130I CCWGG 6 cut(s) 537, 723, 1342, 1913, 2097, 2325
BclI TGATCA 2 cut(s) 967, 1735
BfaI CTAG 8 cut(s) 611, 803, 1535, 1643, 1655, 1757, 1827, 1917
BfmI CTRYAG 6 cut(s) 72, 366, 521, 600, 1311, 2274
BfoI RGCGCY 2 cut(s) 1057, 1774
BfuAI ACCTGC 4 cut(s) 79, 514, 2121, 2139
BglII AGATCT 2 cut(s) 596, 1849
BisI GCNGC 8 cut(s) 328, 1186, 1285, 1447, 1524, 1663, 2274, 2306
BlsI GCNGC 8 cut(s) 329, 1187, 1286, 1448, 1525, 1664, 2275, 2307
BmcAI AGTACT 1 cut(s) 1307
Bme1390I CCNGG 6 cut(s) 537, 723, 1342, 1913, 2097, 2325
Bme18I GGWCC 4 cut(s) 579, 887, 1142, 2151
BmeT110I CYCGRG 1 cut(s) 1797
BmgT120I GGNCC 5 cut(s) 579, 616, 887, 1142, 2151
BmiI GGNNCC 2 cut(s) 795, 1805
BmrFI CCNGG 6 cut(s) 537, 723, 1342, 1913, 2097, 2325
BmrI ACTGGG 2 cut(s) 1169, 1231
BmsI GCATC 5 cut(s) 775, 1535, 1649, 1674, 2040
BmuI ACTGGG 2 cut(s) 1169, 1231
BpiI GAAGAC 1 cut(s) 189
BplI GAGNNNNNCTC 4 cut(s) 1246, 1278, 1292, 1324
BpmI CTGGAG 3 cut(s) 202, 1020, 2075
BpuEI CTTGAG 1 cut(s) 1566
Bsa29I ATCGAT 1 cut(s) 430
BsaBI GATNNNNATC 1 cut(s) 1688
BsaJI CCNNGG 4 cut(s) 201, 436, 535, 890
Bsc4I CCNNNNNNNGG 7 cut(s) 416, 474, 625, 803, 896, 1083, 1084
Bse1I ACTGG 8 cut(s) 185, 385, 421, 757, 889, 1175, 1226, 1593
Bse8I GATNNNNATC 1 cut(s) 1688
BseBI CCWGG 6 cut(s) 537, 723, 1342, 1913, 2097, 2325
BseCI ATCGAT 1 cut(s) 430
BseDI CCNNGG 4 cut(s) 201, 436, 535, 890
BseGI GGATG 9 cut(s) 27, 148, 267, 271, 1231, 1438, 1597, 1664, 2092
BseJI GATNNNNATC 1 cut(s) 1688
BseLI CCNNNNNNNGG 7 cut(s) 416, 474, 625, 803, 896, 1083, 1084
BseMII CTCAG 5 cut(s) 663, 1023, 1823, 1914, 2330
BseNI ACTGG 8 cut(s) 185, 385, 421, 757, 889, 1175, 1226, 1593
BseRI GAGGAG 1 cut(s) 542
BseXI GCAGC 8 cut(s) 339, 1172, 1296, 1433, 1510, 1649, 2260, 2292
BseYI CCCAGC 1 cut(s) 618
BsgI GTGCAG 1 cut(s) 1387
BshFI GGCC 5 cut(s) 360, 617, 1911, 2100, 2260
BshVI ATCGAT 1 cut(s) 430
BsiHKCI CYCGRG 1 cut(s) 1797
BslFI GGGAC 4 cut(s) 191, 1969, 1981, 2195
BslI CCNNNNNNNGG 7 cut(s) 416, 474, 625, 803, 896, 1083, 1084
BsmFI GGGAC 4 cut(s) 191, 1969, 1981, 2195
BsmI GAATGC 4 cut(s) 135, 1133, 1665, 1703
BsnI GGCC 5 cut(s) 360, 617, 1911, 2100, 2260
BsoBI CYCGRG 1 cut(s) 1797
Bsp1286I GDGCHC 1 cut(s) 2347
Bsp19I CCATGG 2 cut(s) 201, 890
BspACI CCGC 1 cut(s) 1287
BspANI GGCC 5 cut(s) 360, 617, 1911, 2100, 2260
BspCNI CTCAG 5 cut(s) 664, 1022, 1824, 1913, 2329
BspDI ATCGAT 1 cut(s) 430
BspHI TCATGA 2 cut(s) 231, 970
BspLI GGNNCC 2 cut(s) 795, 1805
BspMAI CTGCAG 5 cut(s) 76, 525, 604, 1315, 2278
BspMI ACCTGC 4 cut(s) 79, 514, 2121, 2139
BspPI GGATC 6 cut(s) 788, 801, 1021, 1447, 1798, 1811
BsrI ACTGG 8 cut(s) 185, 385, 421, 757, 889, 1175, 1226, 1593
BssECI CCNNGG 4 cut(s) 201, 436, 535, 890
BssT1I CCWWGG 3 cut(s) 201, 436, 890
Bst2UI CCWGG 6 cut(s) 537, 723, 1342, 1913, 2097, 2325
Bst4CI ACNGT 7 cut(s) 229, 319, 1127, 1169, 1397, 1492, 1880
Bst6I CTCTTC 2 cut(s) 1553, 2066
BstC8I GCNNGC 7 cut(s) 917, 1156, 1451, 1703, 1861, 2166, 2211
BstDEI CTNAG 6 cut(s) 672, 1009, 1767, 1832, 1900, 2316
BstDSI CCRYGG 2 cut(s) 201, 890
BstEII GGTNACC 1 cut(s) 526
BstENI CCTNNNNNAGG 1 cut(s) 801
BstF5I GGATG 9 cut(s) 27, 148, 267, 271, 1231, 1438, 1597, 1664, 2092
BstH2I RGCGCY 2 cut(s) 1057, 1774
BstHHI GCGC 2 cut(s) 1056, 1773
BstMWI GCNNNNNNNGC 1 cut(s) 1051
BstNI CCWGG 6 cut(s) 537, 723, 1342, 1913, 2097, 2325
BstNSI RCATGY 6 cut(s) 1158, 1705, 1984, 2126, 2269, 2385
BstPI GGTNACC 1 cut(s) 526
BstSCI CCNGG 6 cut(s) 535, 721, 1340, 1911, 2095, 2323
BstSFI CTRYAG 6 cut(s) 72, 366, 521, 600, 1311, 2274
BstV1I GCAGC 8 cut(s) 339, 1172, 1296, 1433, 1510, 1649, 2260, 2292
BstV2I GAAGAC 1 cut(s) 189
BstX2I RGATCY 5 cut(s) 596, 793, 1439, 1803, 1849
BstXI CCANNNNNNTGG 1 cut(s) 1221
BstYI RGATCY 5 cut(s) 596, 793, 1439, 1803, 1849
Bsu15I ATCGAT 1 cut(s) 430
BsuRI GGCC 5 cut(s) 360, 617, 1911, 2100, 2260
BsuTUI ATCGAT 1 cut(s) 430
BtgI CCRYGG 2 cut(s) 201, 890
BtsCI GGATG 9 cut(s) 27, 148, 267, 271, 1231, 1438, 1597, 1664, 2092
BtsI GCAGTG 2 cut(s) 664, 1320
BtsIMutI CAGTG 5 cut(s) 324, 664, 916, 1320, 1885
BveI ACCTGC 4 cut(s) 79, 514, 2121, 2139
Cac8I GCNNGC 7 cut(s) 917, 1156, 1451, 1703, 1861, 2166, 2211
CaiI CAGNNNCTG 1 cut(s) 671
CciI TCATGA 2 cut(s) 231, 970
CfoI GCGC 2 cut(s) 1056, 1773
Cfr13I GGNCC 5 cut(s) 579, 616, 887, 1142, 2151
ClaI ATCGAT 1 cut(s) 430
CsiI ACCWGGT 2 cut(s) 721, 2323
Csp6I GTAC 6 cut(s) 55, 131, 336, 448, 1306, 2312
CviQI GTAC 6 cut(s) 55, 131, 336, 448, 1306, 2312
DdeI CTNAG 6 cut(s) 672, 1009, 1767, 1832, 1900, 2316
EaeI YGGCCR 1 cut(s) 358
Eam1104I CTCTTC 2 cut(s) 1553, 2066
EarI CTCTTC 2 cut(s) 1553, 2066
Eco130I CCWWGG 3 cut(s) 201, 436, 890
Eco24I GRGCYC 1 cut(s) 2347
Eco32I GATATC 2 cut(s) 459, 1426
Eco47I GGWCC 4 cut(s) 579, 887, 1142, 2151
Eco47III AGCGCT 2 cut(s) 1055, 1772
Eco57I CTGAAG 3 cut(s) 742, 1385, 1613
Eco88I CYCGRG 1 cut(s) 1797
Eco91I GGTNACC 1 cut(s) 526
EcoNI CCTNNNNNAGG 1 cut(s) 801
EcoO65I GGTNACC 1 cut(s) 526
EcoRI GAATTC 2 cut(s) 540, 1883
EcoRII CCWGG 6 cut(s) 535, 721, 1340, 1911, 2095, 2323
EcoRV GATATC 2 cut(s) 459, 1426
EcoT14I CCWWGG 3 cut(s) 201, 436, 890
EcoT22I ATGCAT 3 cut(s) 1135, 1703, 2128
EcoT38I GRGCYC 1 cut(s) 2347
ErhI CCWWGG 3 cut(s) 201, 436, 890
FalI AAGNNNNNCTT 2 cut(s) 483, 515
FaqI GGGAC 4 cut(s) 191, 1969, 1981, 2195
FauNDI CATATG 2 cut(s) 817, 1972
FbaI TGATCA 2 cut(s) 967, 1735
Fnu4HI GCNGC 8 cut(s) 328, 1186, 1285, 1447, 1524, 1663, 2274, 2306
FokI GGATG 9 cut(s) 34, 135, 258, 274, 1238, 1445, 1604, 1671, 2079
FriOI GRGCYC 1 cut(s) 2347
Fsp4HI GCNGC 8 cut(s) 328, 1186, 1285, 1447, 1524, 1663, 2274, 2306
FspBI CTAG 8 cut(s) 611, 803, 1535, 1643, 1655, 1757, 1827, 1917
GlaI GCGC 2 cut(s) 1055, 1772
GluI GCNGC 8 cut(s) 328, 1186, 1285, 1447, 1524, 1663, 2274, 2306
GsaI CCCAGC 1 cut(s) 622
GsuI CTGGAG 3 cut(s) 202, 1020, 2075
HaeII RGCGCY 2 cut(s) 1057, 1774
HaeIII GGCC 5 cut(s) 360, 617, 1911, 2100, 2260
HhaI GCGC 2 cut(s) 1056, 1773
Hin6I GCGC 2 cut(s) 1054, 1771
HinP1I GCGC 2 cut(s) 1054, 1771
HindIII AAGCTT 3 cut(s) 731, 1714, 1747
HphI GGTGA 3 cut(s) 520, 1301, 2306
Hpy166II GTNNAC 1 cut(s) 788
Hpy188I TCNGA 8 cut(s) 66, 153, 905, 1365, 1531, 1903, 2026, 2319
Hpy8I GTNNAC 1 cut(s) 788
HpyAV CCTTC 8 cut(s) 241, 807, 853, 1251, 1360, 2099, 2138, 2291
HpyCH4III ACNGT 7 cut(s) 229, 319, 1127, 1169, 1397, 1492, 1880
HpyCH4IV ACGT 1 cut(s) 2028
HpyF10VI GCNNNNNNNGC 1 cut(s) 1051
HpyF3I CTNAG 6 cut(s) 672, 1009, 1767, 1832, 1900, 2316
HpySE526I ACGT 1 cut(s) 2028
HspAI GCGC 2 cut(s) 1054, 1771
Ksp22I TGATCA 2 cut(s) 967, 1735
LmnI GCTCC 1 cut(s) 780
Lsp1109I GCAGC 8 cut(s) 339, 1172, 1296, 1433, 1510, 1649, 2260, 2292
LweI GCATC 5 cut(s) 775, 1535, 1649, 1674, 2040
MabI ACCWGGT 2 cut(s) 721, 2323
MaeI CTAG 8 cut(s) 611, 803, 1535, 1643, 1655, 1757, 1827, 1917
MaeII ACGT 1 cut(s) 2028
MaeIII GTNAC 6 cut(s) 526, 646, 748, 1289, 1609, 1976
MfeI CAATTG 1 cut(s) 1998
MflI RGATCY 5 cut(s) 596, 793, 1439, 1803, 1849
MhlI GDGCHC 1 cut(s) 2347
MlsI TGGCCA 1 cut(s) 360
MluNI TGGCCA 1 cut(s) 360
MlyI GAGTC 3 cut(s) 203, 1255, 2177
MmeI TCCRAC 1 cut(s) 719
Mox20I TGGCCA 1 cut(s) 360
Mph1103I ATGCAT 3 cut(s) 1135, 1703, 2128
MscI TGGCCA 1 cut(s) 360
MslI CAYNNNNRTG 2 cut(s) 206, 2380
Msp20I TGGCCA 1 cut(s) 360
MspA1I CMGCKG 2 cut(s) 1287, 2273
MspR9I CCNGG 6 cut(s) 537, 723, 1342, 1913, 2097, 2325
MunI CAATTG 1 cut(s) 1998
Mva1269I GAATGC 4 cut(s) 135, 1133, 1665, 1703
MvaI CCWGG 6 cut(s) 537, 723, 1342, 1913, 2097, 2325
MwoI GCNNNNNNNGC 1 cut(s) 1051
NcoI CCATGG 2 cut(s) 201, 890
NdeI CATATG 2 cut(s) 817, 1972
NlaIV GGNNCC 2 cut(s) 795, 1805
NmuCI GTSAC 2 cut(s) 526, 1289
NsiI ATGCAT 3 cut(s) 1135, 1703, 2128
NspI RCATGY 6 cut(s) 1158, 1705, 1984, 2126, 2269, 2385
PaeI GCATGC 2 cut(s) 1158, 1705
PagI TCATGA 2 cut(s) 231, 970
PciI ACATGT 1 cut(s) 1980
PctI GAATGC 4 cut(s) 135, 1133, 1665, 1703
PflMI CCANNNNNTGG 3 cut(s) 474, 625, 896
PkrI GCNGC 8 cut(s) 329, 1187, 1286, 1448, 1525, 1664, 2275, 2307
PleI GAGTC 3 cut(s) 202, 1255, 2177
PpsI GAGTC 3 cut(s) 202, 1255, 2177
PscI ACATGT 1 cut(s) 1980
PshBI ATTAAT 2 cut(s) 1785, 2190
PsiI TTATAA 1 cut(s) 2334
Psp6I CCWGG 6 cut(s) 535, 721, 1340, 1911, 2095, 2323
PspEI GGTNACC 1 cut(s) 526
PspFI CCCAGC 1 cut(s) 618
PspGI CCWGG 6 cut(s) 535, 721, 1340, 1911, 2095, 2323
PspN4I GGNNCC 2 cut(s) 795, 1805
PspPI GGNCC 5 cut(s) 579, 616, 887, 1142, 2151
PsrI GAACNNNNNNTAC 2 cut(s) 1153, 1185
PstI CTGCAG 5 cut(s) 76, 525, 604, 1315, 2278
PstNI CAGNNNCTG 1 cut(s) 671
PsuI RGATCY 5 cut(s) 596, 793, 1439, 1803, 1849
PvuII CAGCTG 1 cut(s) 2273
RsaI GTAC 6 cut(s) 56, 132, 337, 449, 1307, 2313
RsaNI GTAC 6 cut(s) 55, 131, 336, 448, 1306, 2312
RseI CAYNNNNRTG 2 cut(s) 206, 2380
SatI GCNGC 8 cut(s) 328, 1186, 1285, 1447, 1524, 1663, 2274, 2306
Sau96I GGNCC 5 cut(s) 579, 616, 887, 1142, 2151
ScaI AGTACT 1 cut(s) 1307
SchI GAGTC 3 cut(s) 203, 1255, 2177
ScrFI CCNGG 6 cut(s) 537, 723, 1342, 1913, 2097, 2325
SduI GDGCHC 1 cut(s) 2347
SexAI ACCWGGT 2 cut(s) 721, 2323
SfaNI GCATC 5 cut(s) 775, 1535, 1649, 1674, 2040
SfcI CTRYAG 6 cut(s) 72, 366, 521, 600, 1311, 2274
SinI GGWCC 4 cut(s) 579, 887, 1142, 2151
SmiMI CAYNNNNRTG 2 cut(s) 206, 2380
SmlI CTYRAG 1 cut(s) 1581
SmoI CTYRAG 1 cut(s) 1581
SphI GCATGC 2 cut(s) 1158, 1705
SsiI CCGC 1 cut(s) 1287
SspI AATATT 2 cut(s) 983, 1574
SspMI CTAG 8 cut(s) 611, 803, 1535, 1643, 1655, 1757, 1827, 1917
StyD4I CCNGG 6 cut(s) 535, 721, 1340, 1911, 2095, 2323
StyI CCWWGG 3 cut(s) 201, 436, 890
TaaI ACNGT 7 cut(s) 229, 319, 1127, 1169, 1397, 1492, 1880
TaiI ACGT 1 cut(s) 2031
TaqI TCGA 5 cut(s) 218, 430, 1568, 2015, 2415
TatI WGTACW 2 cut(s) 335, 1305
TscAI CASTG 5 cut(s) 324, 664, 916, 1320, 1885
TseFI GTSAC 2 cut(s) 526, 1289
TseI GCWGC 8 cut(s) 327, 1185, 1284, 1446, 1523, 1662, 2273, 2305
Tsp45I GTSAC 2 cut(s) 526, 1289
TspGWI ACGGA 1 cut(s) 1445
TspRI CASTG 5 cut(s) 324, 664, 916, 1320, 1885
Van91I CCANNNNNTGG 3 cut(s) 474, 625, 896
VpaK11BI GGWCC 4 cut(s) 579, 887, 1142, 2151
VspI ATTAAT 2 cut(s) 1785, 2190
XagI CCTNNNNNAGG 1 cut(s) 801
XapI RAATTY 6 cut(s) 540, 849, 1270, 1417, 1483, 1883
XbaI TCTAGA 1 cut(s) 1826
XceI RCATGY 6 cut(s) 1158, 1705, 1984, 2126, 2269, 2385
XcmI CCANNNNNNNNNTGG 1 cut(s) 1585
XspI CTAG 8 cut(s) 611, 803, 1535, 1643, 1655, 1757, 1827, 1917
ZrmI AGTACT 1 cut(s) 1307
Zsp2I ATGCAT 3 cut(s) 1135, 1703, 2128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.