RH5CG202000

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0000484
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG202000.1

Sequence Viewer

Length: 2508 bp
ATGGAAATGAGGTGTTCGAGTATTACAGTCATGAAAAATCCATTGAAGGTTAGGATGTGCTTACATCCTATTTGCTCTTTTCTGCTTATCATCACAGCGATATTTTCCACAGCGGGAGGCAGCACAAGTACATTTCAGTCCATTAGAGATAGCCAGACTATAGTTTCAACTACTGGAAACTTTGAGCTTGGATTTTTTAGTCCTACTGGTTCTAATAATCGATATGTTGGGATATGGTACAAGAAGATATCGTCTACAATAGTATGGGTTGCCAACAGAGAAGCACCCCTTACTGATTCATCAGGTGTTCTGCAGGTCACCGACCCTGGAATTCTTGTCCTCCTCAACCATAACAAGAGCGTAGTTTGGTCCTCAAGCACATCAAGATCTGCAGAGAATCCAGTGGCCCAGCTTTTGGATTCAGGAAATCTTGTTGTAACAGATGGCAGTGATACAGACCCTGAGAACTTCTCGTGGCAAAGTTTTGATTACCCAAGTGATACATTCCTACCAGGTATGAAGCTTGGTTGGAACAAAGTTACAGGCTTCAGTAGGCATCTTACATCATGGAATAGTCCACAGGATCCTTCTCTAGGAAAATATACATATGCACTTGATCGCAGAGGATATGGAGAAGAAATTTTGAAGAAGGAGTCAGTTATGATATGGCGAACTGGACCATGGAATGGAATCGGATTCAGTGGCTTGCCTCATTTAAGTCCAAACTCCGTATTCACATACAATCTTGTTTTTGATGATCATGATGAAGAATATTATATTTTTGAGCTTCATAACAGCTCAGTTTATTCAAGAGTGAGTGTCACTTCAGATGGGCGTGTGCAGCGCTACACATGGATTGATACAACCCAAACTTGGGGATTCCTTTATCAAACAGTTATTGATAACTGCGAAATTTATGCATTCTGTGGTCCAAATGGTGCATGCAGTATTGACAGTTCCCCAGTATGTCGCTGCTTGAATGGATTTCAACCCAAATTTCCAAAGGACTGGGATGTAGAGGATTGGTCAAATGGCTGTGTGAGAAGGACTCCACTAAATTGCAGCGGTGACTTGTTTGAGAAGTACTCTGCAGTGAAATTACCATACACAGAGCAGTCCTGGTTCAACAGAAGTATGAATCTGAAGGAATGTGAGATGGTGTGCCTGAAGAACTGTTCCTGCACAGCTTATGCAAATTTGGATATCACGGATGGAGGATCTGGCTGCCTGCTGTGGTTTGGCAACCTAATTGATATTAGAAATTTTACTGTAAATGCTCAAGATATTTATATAAGAATGGCTGCATCAGAACTAGTTATCAATGCTAAAGACTCTGAATCTAAGAAGGTTAGAATCATACTATGTGGTACTCTGTTGCCTACTGGACTGCTGATCGTGGGCATAGCCCTGATATTTTATTGCCAGAAGAAGCAGCACAAAAAATACGGGAGAATGAGGAGCAGCAAAAAAGAAGGTTCAGAACTTCCCTCACAAAAGGAAGATTTTGAACTTCCATTATTTGACTTGGCTACTGTCATTTGTGCCACGGATAACTTCTCAGAGAACAGCAAACTTGGAGAAGGTGGATTCGGATCCGTCTTTAAGGGTATATTGCAAGATGGACAGGAAATAGCTGTGAAGAGGCTCTCGAAGCATTCTAAACAAGGACTAAATGAGTTCAAGAATGAGGTTACACATATTGTCAAACTTCAGCACAGGAATCTAGTGAAGCTTCTGGGATGCTGCATTCAGGAAGATGAAATGATTCTGATCTACGAATGCATGCCTAACAAAAGCTTGGACTTCTTTATTTTTGATCAAAGAAGAAGCTTTTTACTAGATTGGCCTAAGCGCTTTGACATTATTAATGGTATTGCTCGGGGGATCCTTTATCTTCATCAAGATTCTAGACTGAGGGTAATTCATAGAGATCTAAAAGCAAGCAATATCTTATTAGACAATGAATTCAACCCAAAAATCTCAGACTTTGGCCTGGCTAGAAGTTTTGGAGGAAATGAAACTAAAGCAGAAACGAATAAAGTCGTCGGAACATATGGTTACATGTCCCCAGAATATGCAAATGACGGGTTCTACTCGATAAAGTCAGACGTGTATAGCTTTGGTGTTTTGATGCTGGAGATACTAAGTGGGAAGAGAAACAGAGGATTCCTTCATCCAGGCCACAACCTCAACCTGCTTGGACATGCATGGAACTTACACACAGAAGGCAGGTCCATTGAATTGCTTGCTACATCAGTAGGGGACTCCATTAATCTACAAGAAGTTCTGCGAACGATTCATGTGGGTCTTTTATGTGTTCAGCAAAATCCACAAGACAGGCCAAGCATGTCTGCTGCCGTTCTAATGTTAAGTGGTGAAGGTGTGCTGCCTGTACCTCAGAAACCTGGTTTTTATAATGAGAGGGCTCTAACTGAACTCCAAGTCCAGTCTTCTTCACAACCATGTTCAGTTAATACACTCACTTCCAGCCTATTCGAGCCTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

835

Amino Acids

93.68

Weight (kDa)

6.41

Isoelectric Point (pI)

47.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 86 - 190 5.1e-40 D-mannose binding lectin
S_locus_glycop PF00954 223 - 333 2.3e-29 S-locus glycoprotein domain
PAN_2 PF08276 354 - 419 1.3e-20 PAN-like domain
Pkinase PF00069 520 - 719 1.4e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 522 - 785 2.5e-47 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 793 - 835 7.6e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2415
Acc36I ACCTGC 3 cut(s) 304, 2202, 2220
AccB7I CCANNNNNTGG 2 cut(s) 415, 686
AccI GTMKAC 1 cut(s) 254
AciI CCGC 2 cut(s) 113, 1065
AclWI GGATC 7 cut(s) 578, 591, 1225, 1587, 1600, 1879, 1892
AcsI RAATTY 7 cut(s) 330, 639, 912, 995, 1195, 1261, 1964
AcuI CTGAAG 5 cut(s) 532, 810, 1163, 1187, 1694
AfaI GTAC 5 cut(s) 130, 239, 1085, 1369, 2394
AfeI AGCGCT 2 cut(s) 845, 1853
AfiI CCNNNNNNNGG 5 cut(s) 415, 593, 686, 873, 874
AflIII ACRYGT 2 cut(s) 2061, 2109
AhlI ACTAGT 1 cut(s) 1312
AjiI CACGTC 1 cut(s) 2110
AjnI CCWGG 6 cut(s) 325, 511, 1118, 1992, 2176, 2404
AloI GAACNNNNNNTCC 2 cut(s) 2427, 2459
AlwI GGATC 7 cut(s) 578, 591, 1225, 1587, 1600, 1879, 1892
AlwNI CAGNNNCTG 1 cut(s) 461
Ama87I CYCGRG 1 cut(s) 1878
Aor51HI AGCGCT 2 cut(s) 845, 1853
AoxI GGCC 5 cut(s) 405, 1844, 1990, 2179, 2339
ApoI RAATTY 7 cut(s) 330, 639, 912, 995, 1195, 1261, 1964
AseI ATTAAT 2 cut(s) 1866, 2271
Asp700I GAANNNNTTC 1 cut(s) 1764
AspLEI GCGC 2 cut(s) 846, 1854
AspS9I GGNCC 5 cut(s) 369, 406, 677, 929, 2232
AsuHPI GGTGA 3 cut(s) 310, 1079, 2387
AvaI CYCGRG 1 cut(s) 1878
AvaII GGWCC 4 cut(s) 369, 677, 929, 2232
BamHI GGATCC 3 cut(s) 583, 1592, 1884
BanII GRGCYC 1 cut(s) 2428
BauI CACGAG 1 cut(s) 472
BbsI GAAGAC 1 cut(s) 2442
BccI CCATC 5 cut(s) 437, 824, 1150, 1205, 1613
BceAI ACGGC 1 cut(s) 2342
BcgI CGANNNNNNTGC 2 cut(s) 899, 933
BciT130I CCWGG 6 cut(s) 327, 513, 1120, 1994, 2178, 2406
BclI TGATCA 2 cut(s) 757, 1816
BcuI ACTAGT 1 cut(s) 1312
BfaI CTAG 6 cut(s) 593, 1313, 1724, 1838, 1908, 1998
BfmI CTRYAG 4 cut(s) 159, 311, 390, 1089
BfoI RGCGCY 2 cut(s) 847, 1855
BfuAI ACCTGC 3 cut(s) 304, 2202, 2220
BglII AGATCT 2 cut(s) 386, 1930
BmcAI AGTACT 1 cut(s) 1085
Bme1390I CCNGG 6 cut(s) 327, 513, 1120, 1994, 2178, 2406
Bme18I GGWCC 4 cut(s) 369, 677, 929, 2232
BmeT110I CYCGRG 1 cut(s) 1878
BmgBI CACGTC 1 cut(s) 2110
BmgT120I GGNCC 5 cut(s) 369, 406, 677, 929, 2232
BmiI GGNNCC 3 cut(s) 585, 1594, 1886
BmrFI CCNGG 6 cut(s) 327, 513, 1120, 1994, 2178, 2406
BmrI ACTGGG 2 cut(s) 956, 1018
BmsI GCATC 4 cut(s) 565, 1313, 1730, 2121
BmuI ACTGGG 2 cut(s) 956, 1018
BpiI GAAGAC 1 cut(s) 2442
BplI GAGNNNNNCTC 6 cut(s) 455, 487, 1033, 1065, 1070, 1102
BpmI CTGGAG 1 cut(s) 2156
Bpu10I CCTNAGC 1 cut(s) 1848
BpuEI CTTGAG 2 cut(s) 358, 1263
Bsa29I ATCGAT 1 cut(s) 220
BsaBI GATNNNNATC 2 cut(s) 1591, 1769
BsaJI CCNNGG 3 cut(s) 325, 680, 1545
Bsc4I CCNNNNNNNGG 5 cut(s) 415, 593, 686, 873, 874
Bse1I ACTGG 8 cut(s) 178, 211, 401, 679, 962, 1013, 1387, 2446
Bse8I GATNNNNATC 2 cut(s) 1591, 1769
BseBI CCWGG 6 cut(s) 327, 513, 1120, 1994, 2178, 2406
BseCI ATCGAT 1 cut(s) 220
BseDI CCNNGG 3 cut(s) 325, 680, 1545
BseGI GGATG 6 cut(s) 60, 64, 1018, 1216, 1745, 2173
BseJI GATNNNNATC 2 cut(s) 1591, 1769
BseLI CCNNNNNNNGG 5 cut(s) 415, 593, 686, 873, 874
BseMII CTCAG 6 cut(s) 453, 813, 1572, 1904, 1995, 2411
BseNI ACTGG 8 cut(s) 178, 211, 401, 679, 962, 1013, 1387, 2446
BseRI GAGGAG 2 cut(s) 332, 1471
BseYI CCCAGC 1 cut(s) 408
BsgI GTGCAG 2 cut(s) 860, 1165
BshFI GGCC 5 cut(s) 407, 1846, 1992, 2181, 2341
BshVI ATCGAT 1 cut(s) 220
BsiHKCI CYCGRG 1 cut(s) 1878
BslFI GGGAC 2 cut(s) 2050, 2276
BslI CCNNNNNNNGG 5 cut(s) 415, 593, 686, 873, 874
BsmFI GGGAC 2 cut(s) 2050, 2276
BsmI GAATGC 4 cut(s) 920, 1654, 1746, 1784
BsnI GGCC 5 cut(s) 407, 1846, 1992, 2181, 2341
BsoBI CYCGRG 1 cut(s) 1878
Bsp1286I GDGCHC 1 cut(s) 2428
Bsp19I CCATGG 1 cut(s) 680
BspACI CCGC 2 cut(s) 113, 1065
BspANI GGCC 5 cut(s) 407, 1846, 1992, 2181, 2341
BspCNI CTCAG 6 cut(s) 454, 812, 1571, 1905, 1994, 2410
BspDI ATCGAT 1 cut(s) 220
BspHI TCATGA 2 cut(s) 30, 760
BspLI GGNNCC 3 cut(s) 585, 1594, 1886
BspMAI CTGCAG 3 cut(s) 315, 394, 1093
BspMI ACCTGC 3 cut(s) 304, 2202, 2220
BspPI GGATC 7 cut(s) 578, 591, 1225, 1587, 1600, 1879, 1892
BsrI ACTGG 8 cut(s) 178, 211, 401, 679, 962, 1013, 1387, 2446
BssECI CCNNGG 3 cut(s) 325, 680, 1545
BssSI CACGAG 1 cut(s) 472
BssT1I CCWWGG 1 cut(s) 680
Bst2BI CACGAG 1 cut(s) 472
Bst2UI CCWGG 6 cut(s) 327, 513, 1120, 1994, 2178, 2406
Bst4CI ACNGT 6 cut(s) 28, 895, 956, 1175, 1270, 1534
Bst6I CTCTTC 2 cut(s) 1634, 2147
BstC8I GCNNGC 6 cut(s) 707, 943, 1229, 1784, 1942, 2247
BstDEI CTNAG 9 cut(s) 462, 799, 1341, 1558, 1848, 1913, 1981, 2144, 2397
BstDSI CCRYGG 2 cut(s) 680, 1545
BstEII GGTNACC 1 cut(s) 316
BstENI CCTNNNNNAGG 1 cut(s) 591
BstF5I GGATG 6 cut(s) 60, 64, 1018, 1216, 1745, 2173
BstH2I RGCGCY 2 cut(s) 847, 1855
BstHHI GCGC 2 cut(s) 846, 1854
BstMWI GCNNNNNNNGC 2 cut(s) 841, 1651
BstNI CCWGG 6 cut(s) 327, 513, 1120, 1994, 2178, 2406
BstNSI RCATGY 5 cut(s) 945, 1786, 2065, 2207, 2350
BstPI GGTNACC 1 cut(s) 316
BstSCI CCNGG 6 cut(s) 325, 511, 1118, 1992, 2176, 2404
BstSFI CTRYAG 4 cut(s) 159, 311, 390, 1089
BstV2I GAAGAC 1 cut(s) 2442
BstX2I RGATCY 6 cut(s) 386, 583, 1217, 1592, 1884, 1930
BstXI CCANNNNNNTGG 1 cut(s) 1008
BstYI RGATCY 6 cut(s) 386, 583, 1217, 1592, 1884, 1930
Bsu15I ATCGAT 1 cut(s) 220
BsuRI GGCC 5 cut(s) 407, 1846, 1992, 2181, 2341
BsuTUI ATCGAT 1 cut(s) 220
BtgI CCRYGG 2 cut(s) 680, 1545
BtrI CACGTC 1 cut(s) 2110
BtsCI GGATG 6 cut(s) 60, 64, 1018, 1216, 1745, 2173
BtsI GCAGTG 2 cut(s) 454, 1098
BtsIMutI CAGTG 4 cut(s) 408, 454, 706, 1098
BveI ACCTGC 3 cut(s) 304, 2202, 2220
Cac8I GCNNGC 6 cut(s) 707, 943, 1229, 1784, 1942, 2247
CaiI CAGNNNCTG 1 cut(s) 461
CciI TCATGA 2 cut(s) 30, 760
CfoI GCGC 2 cut(s) 846, 1854
Cfr13I GGNCC 5 cut(s) 369, 406, 677, 929, 2232
ClaI ATCGAT 1 cut(s) 220
CsiI ACCWGGT 2 cut(s) 511, 2404
Csp6I GTAC 5 cut(s) 129, 238, 1084, 1368, 2393
CviQI GTAC 5 cut(s) 129, 238, 1084, 1368, 2393
DdeI CTNAG 9 cut(s) 462, 799, 1341, 1558, 1848, 1913, 1981, 2144, 2397
Eam1104I CTCTTC 2 cut(s) 1634, 2147
EarI CTCTTC 2 cut(s) 1634, 2147
Eco130I CCWWGG 1 cut(s) 680
Eco24I GRGCYC 1 cut(s) 2428
Eco32I GATATC 2 cut(s) 249, 1204
Eco47I GGWCC 4 cut(s) 369, 677, 929, 2232
Eco47III AGCGCT 2 cut(s) 845, 1853
Eco57I CTGAAG 5 cut(s) 532, 810, 1163, 1187, 1694
Eco88I CYCGRG 1 cut(s) 1878
Eco91I GGTNACC 1 cut(s) 316
EcoNI CCTNNNNNAGG 1 cut(s) 591
EcoO65I GGTNACC 1 cut(s) 316
EcoRI GAATTC 2 cut(s) 330, 1964
EcoRII CCWGG 6 cut(s) 325, 511, 1118, 1992, 2176, 2404
EcoRV GATATC 2 cut(s) 249, 1204
EcoT14I CCWWGG 1 cut(s) 680
EcoT22I ATGCAT 3 cut(s) 922, 1784, 2209
EcoT38I GRGCYC 1 cut(s) 2428
ErhI CCWWGG 1 cut(s) 680
FalI AAGNNNNNCTT 2 cut(s) 273, 305
FaqI GGGAC 2 cut(s) 2050, 2276
FauI CCCGC 1 cut(s) 106
FauNDI CATATG 2 cut(s) 607, 2053
FbaI TGATCA 2 cut(s) 757, 1816
FblI GTMKAC 1 cut(s) 254
FokI GGATG 6 cut(s) 51, 67, 1025, 1223, 1752, 2160
FriOI GRGCYC 1 cut(s) 2428
FspBI CTAG 6 cut(s) 593, 1313, 1724, 1838, 1908, 1998
GlaI GCGC 2 cut(s) 845, 1853
GsaI CCCAGC 1 cut(s) 412
GsuI CTGGAG 1 cut(s) 2156
HaeII RGCGCY 2 cut(s) 847, 1855
HaeIII GGCC 5 cut(s) 407, 1846, 1992, 2181, 2341
HhaI GCGC 2 cut(s) 846, 1854
Hin6I GCGC 2 cut(s) 844, 1852
HinP1I GCGC 2 cut(s) 844, 1852
HindIII AAGCTT 4 cut(s) 521, 1730, 1795, 1828
HphI GGTGA 3 cut(s) 310, 1079, 2387
Hpy166II GTNNAC 2 cut(s) 255, 578
Hpy8I GTNNAC 2 cut(s) 255, 578
Hpy99I CGWCG 1 cut(s) 2048
HpyCH4III ACNGT 6 cut(s) 28, 895, 956, 1175, 1270, 1534
HpyCH4IV ACGT 1 cut(s) 2109
HpyF10VI GCNNNNNNNGC 2 cut(s) 841, 1651
HpyF3I CTNAG 9 cut(s) 462, 799, 1341, 1558, 1848, 1913, 1981, 2144, 2397
HpySE526I ACGT 1 cut(s) 2109
HspAI GCGC 2 cut(s) 844, 1852
Ksp22I TGATCA 2 cut(s) 757, 1816
LmnI GCTCC 1 cut(s) 1458
LweI GCATC 4 cut(s) 565, 1313, 1730, 2121
MabI ACCWGGT 2 cut(s) 511, 2404
MaeI CTAG 6 cut(s) 593, 1313, 1724, 1838, 1908, 1998
MaeII ACGT 1 cut(s) 2109
MaeIII GTNAC 7 cut(s) 316, 436, 538, 820, 1067, 1690, 2057
MflI RGATCY 6 cut(s) 386, 583, 1217, 1592, 1884, 1930
MhlI GDGCHC 1 cut(s) 2428
MlyI GAGTC 4 cut(s) 662, 1042, 1325, 2258
MmeI TCCRAC 2 cut(s) 509, 2026
Mph1103I ATGCAT 3 cut(s) 922, 1784, 2209
MroXI GAANNNNTTC 1 cut(s) 1764
MseI TTAA 7 cut(s) 716, 1602, 1866, 2271, 2369, 2472, 2506
MslI CAYNNNNRTG 1 cut(s) 2461
MspA1I CMGCKG 2 cut(s) 113, 1065
MspR9I CCNGG 6 cut(s) 327, 513, 1120, 1994, 2178, 2406
Mva1269I GAATGC 4 cut(s) 920, 1654, 1746, 1784
MvaI CCWGG 6 cut(s) 327, 513, 1120, 1994, 2178, 2406
MwoI GCNNNNNNNGC 2 cut(s) 841, 1651
NcoI CCATGG 1 cut(s) 680
NdeI CATATG 2 cut(s) 607, 2053
NlaIV GGNNCC 3 cut(s) 585, 1594, 1886
NmuCI GTSAC 3 cut(s) 316, 820, 1067
NsiI ATGCAT 3 cut(s) 922, 1784, 2209
NspI RCATGY 5 cut(s) 945, 1786, 2065, 2207, 2350
PaeI GCATGC 2 cut(s) 945, 1786
PagI TCATGA 2 cut(s) 30, 760
PciI ACATGT 1 cut(s) 2061
PctI GAATGC 4 cut(s) 920, 1654, 1746, 1784
PdmI GAANNNNTTC 1 cut(s) 1764
PflMI CCANNNNNTGG 2 cut(s) 415, 686
PleI GAGTC 4 cut(s) 661, 1042, 1325, 2258
PpsI GAGTC 4 cut(s) 661, 1042, 1325, 2258
PscI ACATGT 1 cut(s) 2061
PshBI ATTAAT 2 cut(s) 1866, 2271
PsiI TTATAA 1 cut(s) 2415
Psp6I CCWGG 6 cut(s) 325, 511, 1118, 1992, 2176, 2404
PspEI GGTNACC 1 cut(s) 316
PspFI CCCAGC 1 cut(s) 408
PspGI CCWGG 6 cut(s) 325, 511, 1118, 1992, 2176, 2404
PspN4I GGNNCC 3 cut(s) 585, 1594, 1886
PspPI GGNCC 5 cut(s) 369, 406, 677, 929, 2232
PsrI GAACNNNNNNTAC 2 cut(s) 940, 972
PstI CTGCAG 3 cut(s) 315, 394, 1093
PstNI CAGNNNCTG 1 cut(s) 461
PsuI RGATCY 6 cut(s) 386, 583, 1217, 1592, 1884, 1930
RsaI GTAC 5 cut(s) 130, 239, 1085, 1369, 2394
RsaNI GTAC 5 cut(s) 129, 238, 1084, 1368, 2393
RseI CAYNNNNRTG 1 cut(s) 2461
SaqAI TTAA 7 cut(s) 716, 1602, 1866, 2271, 2369, 2472, 2506
Sau96I GGNCC 5 cut(s) 369, 406, 677, 929, 2232
ScaI AGTACT 1 cut(s) 1085
SchI GAGTC 4 cut(s) 662, 1042, 1325, 2258
ScrFI CCNGG 6 cut(s) 327, 513, 1120, 1994, 2178, 2406
SduI GDGCHC 1 cut(s) 2428
SexAI ACCWGGT 2 cut(s) 511, 2404
SfaNI GCATC 4 cut(s) 565, 1313, 1730, 2121
SfcI CTRYAG 4 cut(s) 159, 311, 390, 1089
SinI GGWCC 4 cut(s) 369, 677, 929, 2232
SmiMI CAYNNNNRTG 1 cut(s) 2461
SmlI CTYRAG 2 cut(s) 373, 1278
SmoI CTYRAG 2 cut(s) 373, 1278
SpeI ACTAGT 1 cut(s) 1312
SphI GCATGC 2 cut(s) 945, 1786
SsiI CCGC 2 cut(s) 113, 1065
SspI AATATT 1 cut(s) 773
SspMI CTAG 6 cut(s) 593, 1313, 1724, 1838, 1908, 1998
StyD4I CCNGG 6 cut(s) 325, 511, 1118, 1992, 2176, 2404
StyI CCWWGG 1 cut(s) 680
TaaI ACNGT 6 cut(s) 28, 895, 956, 1175, 1270, 1534
TaiI ACGT 1 cut(s) 2112
TaqI TCGA 5 cut(s) 17, 220, 1649, 2096, 2496
TatI WGTACW 2 cut(s) 128, 1083
Tru1I TTAA 7 cut(s) 716, 1602, 1866, 2271, 2369, 2472, 2506
Tru9I TTAA 7 cut(s) 716, 1602, 1866, 2271, 2369, 2472, 2506
TscAI CASTG 4 cut(s) 408, 454, 706, 1098
TseFI GTSAC 3 cut(s) 316, 820, 1067
Tsp45I GTSAC 3 cut(s) 316, 820, 1067
TspGWI ACGGA 4 cut(s) 718, 1223, 1562, 1585
TspRI CASTG 4 cut(s) 408, 454, 706, 1098
Van91I CCANNNNNTGG 2 cut(s) 415, 686
VpaK11BI GGWCC 4 cut(s) 369, 677, 929, 2232
VspI ATTAAT 2 cut(s) 1866, 2271
XagI CCTNNNNNAGG 1 cut(s) 591
XapI RAATTY 7 cut(s) 330, 639, 912, 995, 1195, 1261, 1964
XbaI TCTAGA 1 cut(s) 1907
XceI RCATGY 5 cut(s) 945, 1786, 2065, 2207, 2350
XmiI GTMKAC 1 cut(s) 254
XmnI GAANNNNTTC 1 cut(s) 1764
XspI CTAG 6 cut(s) 593, 1313, 1724, 1838, 1908, 1998
ZrmI AGTACT 1 cut(s) 1085
Zsp2I ATGCAT 3 cut(s) 922, 1784, 2209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.