MD05G1232400.v1.1

G-type lectin S-receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000484
Physical Location & Seq
Forward (+)
36525505 .. 36528771
3267 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1232400.v1.1.491

Sequence Viewer

Length: 2352 bp
ATGAGACGGACGATTGAAACCTTCAACATTCAGTGCTTTCCCATGAAGTTTATGAAAAATCCGTCAAAAGCTTCAGCTAAGATGTGCTTGCTTCTTTTCTACTCTTCTTTGCTGCTCATCGTCGCAGCAGTATTCTCCAAAGCTGATGACACCACGAGTACATTTCAGTCCATTAGAGACGGTGAGACTGTAGTTTCAACTGGCGGTACGTTTGAGCTAGGATTTTACAATCTTGATGCTTCTAATGGACGGTACGTGGCGATATGGTACAAGCAGATATCTGTTAAAACCATTGTATGGGTTGCCAACAGAGACAAGCCCCTCTCTGATTTGTCCGGCTTTTTGAAGGTCACCAACCCCGGAATTCTTGTCCTCAACCACAACATGAGCACAGTTTGGTCCTCCAACACATCGAGAACTGCACAGAATCCAGTAGCACGTCTTTTGGATTCGGGTAATCTTGTCGTGATAGATAGGAGTGATGATGACTCTGAGAACTTTCTGTGGCAAAGTTTTGATTACCCTGGCGATACATTCTTACCAGGTATGAAGATTGGTAGGAACACAGTTACAGGCTTCAATTGGCATCTTAGATCGTGGAAAAGTCCTCATGATCCTTCTCAAGGAAATTGTACATCTCAACTTGGTCCCAAAGGATATGCAGAATTATTTGTGAGGGAAGGTTCGATCCTTAAATTTCGGTCTGGACCATGGAATGGAGTCTGGTTCAGTGGAAACCCTCAGTTAAATCCAAACCCTATATACACGTACAGTCTTGTTTCTGAGCCTGATGAAATGTACTACAGTTACAAGCTTCACAATAGCTCAATCTTTTCGAGGTTGATGTTAACTTCAGATGGACTTCTGCGGCGCTACACATGGATTGATAGAACCCAAGGTTGGGTTCTTTACCAAACAGTCCAAATTGATAACTGTGACAACTATGCATTATGTGGTGTACATGGTGCATGTAACATTCAAAAGTCCCCGGTTTGTAGCTGTTTGAAAGGATTTACACCAAAGTTTCCGAAAGAATGGGATTTGGTAGATTGGTCTAATGGTTGTGTGAGAAAAACTTCTCTAAATTGCACCGGAGACGTGTTCCAAAAGTACTCGGGTTTGAAATTGCCTAGCACAGAACAATCCTGGTTTAACAGAAGTATGAACCTCAAGGAATGTGAGATGGTGTGCATGAAGAACTGCTCCTGCACGGCTTATACAAATTTGGATATCCGTGATGGAGGAACTGGGTGCTTGCTGTGGTACGGTGATCTAATTGATATAAGATACATTGCTGAAAACGGGCAAGATATTTATATAAGAATGGCCGCAGCAGAACAAGGAAAACTGGGACGCAACCAAAAGGAGGATTTGGAATTACCGTTATTTGACTTGATGACTATAGTTTCTGCAACCAGTAACTTTTCAATTGAAAACAAACTTGGTGAAGGTGGTTTCGGATCTGTCTTTAAGGGTACGATGGAAAATGGACAAGAAATCGCAGTGAAAAGGCTTTCAAAAAGTTCTAGACAAGGACTCGACGAGTTCAAGAATGAAGTTACACATATTGCCAAACTTCAGCATAGGAATCTAGTGAAGCTTCTTGGATGCTGCATTCAAGAAGACGAGATGATACTGATCTACGAGTTCATGCCCAACAAGAGTTTAGACTTCTTTATTTTTGACTGGCCTAAGCGCTTTGAAATTATTAATGGGATAGCTCGAGGGATCCTCTATCTTCATCAAGATTCTAGATTGAGAGTTATTCATAGAGATCTCAAAGCAAGTAACATCTTATTAGGCAGTGAATTTAACCCAAAAATCTCAGACTTTGGCCTAGCTAGAAGCTTTGGAGGAAATGAAACGACAGCAAAAACGAAGAAAGTGGTTGGAAAATACGGTTACATGTCCCCAGAATATGTAATTGATGGTTTCTACTCTATAAAGTCCGACGTCTATAGCTTTGGTGTTATGGTGCTAGAGATAGTGAGTGGGAGCAGAAATAGAGGATTCTCTCATCCAGACCACAAACTCAACCTTCTTGGACATGCATGGATGCTACACACAAAAGACATGCCTCTCAAACTGATTGATGCATCGGTAGAGGACTCCGTCACTTTGCATGAAGTTGTAAGAACGATTCATGTGGGTCTTTTATGCGTGCAGCGGAATCCAGGCGACCGGCCGAGCATGTCAGCTGTAGTTCTAATGTTGGGTGGTGAAGGTGCGTTGCCCCCACCTCTAAAACCTAGTTTTTACAGTGAAAGGGATCTGACTGAACTTGAAGTCAGTCATTCTTCTAAAGCATGCTCAGCTAATGAAGTCACTATTTCACTAGTTGAGGCTCGATAA

Protein Analysis

784

Amino Acids

88.07

Weight (kDa)

7.76

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 97 - 201 6.6e-38 D-mannose binding lectin
S_locus_glycop PF00954 233 - 342 7.5e-31 S-locus glycoprotein domain
PAN_2 PF08276 363 - 428 5.3e-20 PAN-like domain
PK_Tyr_Ser-Thr PF07714 476 - 735 7.7e-46 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 476 - 733 1.4e-43 Protein kinase domain
DUF3403 PF11883 742 - 783 4.7e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1956
AccB7I CCANNNNNTGG 2 cut(s) 297, 716
AciI CCGC 4 cut(s) 204, 868, 1329, 2167
AclWI GGATC 6 cut(s) 608, 682, 1468, 1723, 1736, 2277
AcoI YGGCCR 2 cut(s) 1326, 2183
AcsI RAATTY 4 cut(s) 363, 695, 1222, 1808
AcuI CTGAAG 3 cut(s) 57, 837, 1562
AcyI GRCGYC 1 cut(s) 1953
AfeI AGCGCT 1 cut(s) 1697
AfiI CCNNNNNNNGG 6 cut(s) 297, 623, 716, 900, 901, 1366
AflIII ACRYGT 3 cut(s) 765, 1098, 1905
AhlI ACTAGT 1 cut(s) 2335
AjiI CACGTC 2 cut(s) 440, 1099
AjnI CCWGG 4 cut(s) 523, 541, 1145, 2173
AjuI GAANNNNNNNTTGG 6 cut(s) 883, 888, 915, 920, 1425, 1457
Alw21I GWGCWC 1 cut(s) 392
Alw26I GTCTC 4 cut(s) 171, 179, 306, 1089
AlwI GGATC 6 cut(s) 608, 682, 1468, 1723, 1736, 2277
Ama87I CYCGRG 2 cut(s) 1114, 1722
Aor51HI AGCGCT 1 cut(s) 1697
AoxI GGCC 4 cut(s) 1326, 1688, 1834, 2183
ApeKI GCWGC 5 cut(s) 112, 125, 1331, 1611, 2164
ApoI RAATTY 4 cut(s) 363, 695, 1222, 1808
AseI ATTAAT 1 cut(s) 1710
Asp700I GAANNNNTTC 1 cut(s) 1075
AspLEI GCGC 2 cut(s) 873, 1698
AspS9I GGNCC 3 cut(s) 399, 647, 707
AsuC2I CCSGG 2 cut(s) 360, 989
AsuHPI GGTGA 5 cut(s) 194, 343, 1280, 1457, 2231
AvaI CYCGRG 2 cut(s) 1114, 1722
AvaII GGWCC 3 cut(s) 399, 647, 707
BamHI GGATCC 1 cut(s) 1728
BauI CACGAG 1 cut(s) 154
BbsI GAAGAC 1 cut(s) 1629
Bbv12I GWGCWC 1 cut(s) 392
BbvI GCAGC 5 cut(s) 99, 137, 1343, 1598, 2176
BccI CCATC 5 cut(s) 851, 1177, 1232, 1474, 1922
BceAI ACGGC 1 cut(s) 1227
BciT130I CCWGG 4 cut(s) 525, 543, 1147, 2175
BcnI CCSGG 2 cut(s) 360, 989
BcoDI GTCTC 4 cut(s) 171, 179, 306, 1089
BcuI ACTAGT 1 cut(s) 2335
BfmI CTRYAG 5 cut(s) 189, 802, 1401, 1957, 2199
BfoI RGCGCY 2 cut(s) 874, 1699
BglII AGATCT 1 cut(s) 1774
BisI GCNGC 7 cut(s) 113, 126, 869, 1329, 1332, 1612, 2165
BlpI GCTNAGC 1 cut(s) 2311
BlsI GCNGC 7 cut(s) 114, 127, 870, 1330, 1333, 1613, 2166
BmcAI AGTACT 1 cut(s) 1112
Bme1390I CCNGG 6 cut(s) 360, 525, 543, 989, 1147, 2175
Bme18I GGWCC 3 cut(s) 399, 647, 707
BmeT110I CYCGRG 2 cut(s) 1114, 1722
BmgBI CACGTC 2 cut(s) 440, 1099
BmgT120I GGNCC 3 cut(s) 399, 647, 707
BmiI GGNNCC 2 cut(s) 649, 1730
BmrFI CCNGG 6 cut(s) 360, 525, 543, 989, 1147, 2175
BmrI ACTGGG 2 cut(s) 1257, 1358
BmsI GCATC 6 cut(s) 226, 595, 1598, 2046, 2083, 2105
BmuI ACTGGG 2 cut(s) 1257, 1358
BpiI GAAGAC 1 cut(s) 1629
BplI GAGNNNNNCTC 2 cut(s) 1716, 1748
Bpu10I CCTNAGC 1 cut(s) 1692
Bpu1102I GCTNAGC 1 cut(s) 2311
BpuEI CTTGAG 2 cut(s) 606, 1154
BpuMI CCSGG 2 cut(s) 360, 989
BsaAI YACGTR 2 cut(s) 256, 768
BsaBI GATNNNNATC 1 cut(s) 1637
BsaHI GRCGYC 1 cut(s) 1953
BsaJI CCNNGG 5 cut(s) 358, 523, 710, 895, 987
BsaWI WCCGGW 1 cut(s) 1091
Bsc4I CCNNNNNNNGG 6 cut(s) 297, 623, 716, 900, 901, 1366
Bse118I RCCGGY 1 cut(s) 2181
Bse1I ACTGG 6 cut(s) 205, 431, 1252, 1353, 1416, 1691
Bse3DI GCAATG 1 cut(s) 1290
Bse8I GATNNNNATC 1 cut(s) 1637
BseBI CCWGG 4 cut(s) 525, 543, 1147, 2175
BseDI CCNNGG 5 cut(s) 358, 523, 710, 895, 987
BseGI GGATG 3 cut(s) 1613, 2017, 2061
BseJI GATNNNNATC 1 cut(s) 1637
BseLI CCNNNNNNNGG 6 cut(s) 297, 623, 716, 900, 901, 1366
BseMI GCAATG 1 cut(s) 1290
BseMII CTCAG 5 cut(s) 483, 755, 774, 1839, 2325
BseNI ACTGG 6 cut(s) 205, 431, 1252, 1353, 1416, 1691
BseX3I CGGCCG 1 cut(s) 2183
BseXI GCAGC 5 cut(s) 99, 137, 1343, 1598, 2176
BsgI GTGCAG 3 cut(s) 405, 1192, 2183
Bsh1285I CGRYCG 2 cut(s) 2182, 2186
BshFI GGCC 4 cut(s) 1328, 1690, 1836, 2185
BsiEI CGRYCG 2 cut(s) 2182, 2186
BsiHKAI GWGCWC 1 cut(s) 392
BsiHKCI CYCGRG 2 cut(s) 1114, 1722
BsiSI CCGG 5 cut(s) 336, 360, 989, 1092, 2182
BslFI GGGAC 4 cut(s) 633, 970, 1365, 1894
BslI CCNNNNNNNGG 6 cut(s) 297, 623, 716, 900, 901, 1366
BsmAI GTCTC 4 cut(s) 171, 179, 306, 1089
BsmBI CGTCTC 2 cut(s) 171, 1089
BsmFI GGGAC 4 cut(s) 633, 970, 1365, 1894
BsmI GAATGC 1 cut(s) 1614
BsnI GGCC 4 cut(s) 1328, 1690, 1836, 2185
BsoBI CYCGRG 2 cut(s) 1114, 1722
Bsp1286I GDGCHC 1 cut(s) 392
Bsp1407I TGTACA 2 cut(s) 632, 958
Bsp143I GATC 9 cut(s) 593, 613, 687, 1270, 1460, 1638, 1728, 1774, 2269
Bsp1720I GCTNAGC 1 cut(s) 2311
Bsp19I CCATGG 1 cut(s) 710
BspACI CCGC 4 cut(s) 204, 868, 1329, 2167
BspANI GGCC 4 cut(s) 1328, 1690, 1836, 2185
BspCNI CTCAG 5 cut(s) 484, 754, 775, 1838, 2324
BspHI TCATGA 1 cut(s) 610
BspLI GGNNCC 2 cut(s) 649, 1730
BspPI GGATC 6 cut(s) 608, 682, 1468, 1723, 1736, 2277
BsrDI GCAATG 1 cut(s) 1290
BsrFI RCCGGY 1 cut(s) 2181
BsrGI TGTACA 2 cut(s) 632, 958
BsrI ACTGG 6 cut(s) 205, 431, 1252, 1353, 1416, 1691
BssAI RCCGGY 1 cut(s) 2181
BssECI CCNNGG 5 cut(s) 358, 523, 710, 895, 987
BssMI GATC 9 cut(s) 593, 613, 687, 1270, 1460, 1638, 1728, 1774, 2269
BssNI GRCGYC 1 cut(s) 1953
BssSI CACGAG 1 cut(s) 154
BssT1I CCWWGG 2 cut(s) 710, 895
Bst2BI CACGAG 1 cut(s) 154
Bst2UI CCWGG 4 cut(s) 525, 543, 1147, 2175
Bst6I CTCTTC 1 cut(s) 109
BstACI GRCGYC 1 cut(s) 1953
BstAUI TGTACA 2 cut(s) 632, 958
BstBAI YACGTR 2 cut(s) 256, 768
BstC8I GCNNGC 4 cut(s) 89, 1256, 2162, 2308
BstDEI CTNAG 8 cut(s) 78, 492, 590, 741, 783, 1692, 1825, 2311
BstDSI CCRYGG 1 cut(s) 710
BstEII GGTNACC 1 cut(s) 349
BstENI CCTNNNNNAGG 1 cut(s) 621
BstF5I GGATG 3 cut(s) 1613, 2017, 2061
BstH2I RGCGCY 2 cut(s) 874, 1699
BstHHI GCGC 2 cut(s) 873, 1698
BstKTI GATC 9 cut(s) 596, 616, 690, 1273, 1463, 1641, 1731, 1777, 2272
BstMAI GTCTC 4 cut(s) 171, 179, 306, 1089
BstMBI GATC 9 cut(s) 593, 613, 687, 1270, 1460, 1638, 1728, 1774, 2269
BstMCI CGRYCG 2 cut(s) 2182, 2186
BstMWI GCNNNNNNNGC 1 cut(s) 2312
BstNI CCWGG 4 cut(s) 525, 543, 1147, 2175
BstNSI RCATGY 6 cut(s) 972, 1909, 2051, 2077, 2194, 2310
BstPI GGTNACC 1 cut(s) 349
BstSCI CCNGG 6 cut(s) 358, 523, 541, 987, 1145, 2173
BstSFI CTRYAG 5 cut(s) 189, 802, 1401, 1957, 2199
BstV1I GCAGC 5 cut(s) 99, 137, 1343, 1598, 2176
BstV2I GAAGAC 1 cut(s) 1629
BstX2I RGATCY 4 cut(s) 1460, 1728, 1774, 2269
BstYI RGATCY 4 cut(s) 1460, 1728, 1774, 2269
BstZI CGGCCG 1 cut(s) 2183
BsuRI GGCC 4 cut(s) 1328, 1690, 1836, 2185
BtgI CCRYGG 1 cut(s) 710
BtrI CACGTC 2 cut(s) 440, 1099
BtsCI GGATG 3 cut(s) 1613, 2017, 2061
BtsI GCAGTG 2 cut(s) 1509, 1810
BtsIMutI CAGTG 5 cut(s) 38, 736, 1509, 1810, 2266
Cac8I GCNNGC 4 cut(s) 89, 1256, 2162, 2308
CciI TCATGA 1 cut(s) 610
CfoI GCGC 2 cut(s) 873, 1698
Cfr10I RCCGGY 1 cut(s) 2181
Cfr13I GGNCC 3 cut(s) 399, 647, 707
CseI GACGC 1 cut(s) 1362
CsiI ACCWGGT 1 cut(s) 541
DdeI CTNAG 8 cut(s) 78, 492, 590, 741, 783, 1692, 1825, 2311
DpnI GATC 9 cut(s) 595, 615, 689, 1272, 1462, 1640, 1730, 1776, 2271
DpnII GATC 9 cut(s) 593, 613, 687, 1270, 1460, 1638, 1728, 1774, 2269
EaeI YGGCCR 2 cut(s) 1326, 2183
EagI CGGCCG 1 cut(s) 2183
Eam1104I CTCTTC 1 cut(s) 109
EarI CTCTTC 1 cut(s) 109
EclXI CGGCCG 1 cut(s) 2183
Eco130I CCWWGG 2 cut(s) 710, 895
Eco32I GATATC 2 cut(s) 279, 1231
Eco47I GGWCC 3 cut(s) 399, 647, 707
Eco47III AGCGCT 1 cut(s) 1697
Eco52I CGGCCG 1 cut(s) 2183
Eco57I CTGAAG 3 cut(s) 57, 837, 1562
Eco88I CYCGRG 2 cut(s) 1114, 1722
Eco91I GGTNACC 1 cut(s) 349
EcoNI CCTNNNNNAGG 1 cut(s) 621
EcoO65I GGTNACC 1 cut(s) 349
EcoRI GAATTC 1 cut(s) 363
EcoRII CCWGG 4 cut(s) 523, 541, 1145, 2173
EcoRV GATATC 2 cut(s) 279, 1231
EcoT14I CCWWGG 2 cut(s) 710, 895
EcoT22I ATGCAT 3 cut(s) 949, 2053, 2098
ErhI CCWWGG 2 cut(s) 710, 895
Esp3I CGTCTC 2 cut(s) 171, 1089
FalI AAGNNNNNCTT 2 cut(s) 71, 103
FaqI GGGAC 4 cut(s) 633, 970, 1365, 1894
Fnu4HI GCNGC 7 cut(s) 113, 126, 869, 1329, 1332, 1612, 2165
FokI GGATG 3 cut(s) 1620, 2004, 2068
Fsp4HI GCNGC 7 cut(s) 113, 126, 869, 1329, 1332, 1612, 2165
GlaI GCGC 2 cut(s) 872, 1697
GluI GCNGC 7 cut(s) 113, 126, 869, 1329, 1332, 1612, 2165
HaeII RGCGCY 2 cut(s) 874, 1699
HaeIII GGCC 4 cut(s) 1328, 1690, 1836, 2185
HapII CCGG 5 cut(s) 336, 360, 989, 1092, 2182
HgaI GACGC 1 cut(s) 1362
HhaI GCGC 2 cut(s) 873, 1698
Hin1I GRCGYC 1 cut(s) 1953
Hin6I GCGC 2 cut(s) 871, 1696
HinP1I GCGC 2 cut(s) 871, 1696
HincII GTYRAC 1 cut(s) 849
HindII GTYRAC 1 cut(s) 849
HindIII AAGCTT 4 cut(s) 69, 812, 1598, 1846
HpaI GTTAAC 1 cut(s) 849
HpaII CCGG 5 cut(s) 336, 360, 989, 1092, 2182
HphI GGTGA 5 cut(s) 194, 343, 1280, 1457, 2231
Hpy166II GTNNAC 2 cut(s) 849, 959
Hpy188I TCNGA 9 cut(s) 328, 493, 784, 856, 1029, 1460, 1828, 1951, 2274
Hpy8I GTNNAC 2 cut(s) 849, 959
Hpy99I CGWCG 3 cut(s) 125, 1544, 1955
HpyAV CCTTC 7 cut(s) 31, 340, 627, 674, 1442, 2048, 2216
HpyCH4IV ACGT 6 cut(s) 209, 255, 439, 767, 1098, 1953
HpyF10VI GCNNNNNNNGC 1 cut(s) 2312
HpyF3I CTNAG 8 cut(s) 78, 492, 590, 741, 783, 1692, 1825, 2311
HpySE526I ACGT 6 cut(s) 209, 255, 439, 767, 1098, 1953
Hsp92I GRCGYC 1 cut(s) 1953
HspAI GCGC 2 cut(s) 871, 1696
KspAI GTTAAC 1 cut(s) 849
Kzo9I GATC 9 cut(s) 593, 613, 687, 1270, 1460, 1638, 1728, 1774, 2269
LmnI GCTCC 2 cut(s) 1208, 1995
Lsp1109I GCAGC 5 cut(s) 99, 137, 1343, 1598, 2176
LweI GCATC 6 cut(s) 226, 595, 1598, 2046, 2083, 2105
MabI ACCWGGT 1 cut(s) 541
MaeII ACGT 6 cut(s) 209, 255, 439, 767, 1098, 1953
MalI GATC 9 cut(s) 595, 615, 689, 1272, 1462, 1640, 1730, 1776, 2271
MboI GATC 9 cut(s) 593, 613, 687, 1270, 1460, 1638, 1728, 1774, 2269
MboII GAAGA 7 cut(s) 96, 562, 1207, 1634, 1730, 1891, 2289
MfeI CAATTG 2 cut(s) 580, 1428
MflI RGATCY 4 cut(s) 1460, 1728, 1774, 2269
MhlI GDGCHC 1 cut(s) 392
MlyI GAGTC 4 cut(s) 482, 729, 1530, 2102
MmeI TCCRAC 3 cut(s) 429, 1870, 1974
Mph1103I ATGCAT 3 cut(s) 949, 2053, 2098
MroXI GAANNNNTTC 1 cut(s) 1075
MseI TTAA 8 cut(s) 285, 693, 746, 848, 1152, 1470, 1710, 1812
MspA1I CMGCKG 2 cut(s) 2167, 2198
MspI CCGG 5 cut(s) 336, 360, 989, 1092, 2182
MspR9I CCNGG 6 cut(s) 360, 525, 543, 989, 1147, 2175
MunI CAATTG 2 cut(s) 580, 1428
Mva1269I GAATGC 1 cut(s) 1614
MvaI CCWGG 4 cut(s) 525, 543, 1147, 2175
MwoI GCNNNNNNNGC 1 cut(s) 2312
NciI CCSGG 2 cut(s) 360, 989
NcoI CCATGG 1 cut(s) 710
NdeII GATC 9 cut(s) 593, 613, 687, 1270, 1460, 1638, 1728, 1774, 2269
NlaIV GGNNCC 2 cut(s) 649, 1730
NmeAIII GCCGAG 1 cut(s) 2211
NmuCI GTSAC 4 cut(s) 349, 935, 2113, 2323
NsiI ATGCAT 3 cut(s) 949, 2053, 2098
NspI RCATGY 6 cut(s) 972, 1909, 2051, 2077, 2194, 2310
PaeI GCATGC 1 cut(s) 2310
PaeR7I CTCGAG 1 cut(s) 1722
PagI TCATGA 1 cut(s) 610
PciI ACATGT 1 cut(s) 1905
PctI GAATGC 1 cut(s) 1614
PdmI GAANNNNTTC 1 cut(s) 1075
PfeI GAWTC 7 cut(s) 427, 449, 1588, 1748, 2010, 2140, 2170
PflFI GACNNNGTC 1 cut(s) 2111
PflMI CCANNNNNTGG 2 cut(s) 297, 716
PkrI GCNGC 7 cut(s) 114, 127, 870, 1330, 1333, 1613, 2166
PleI GAGTC 4 cut(s) 482, 728, 1530, 2102
PpsI GAGTC 4 cut(s) 482, 728, 1530, 2102
Ppu21I YACGTR 2 cut(s) 256, 768
PscI ACATGT 1 cut(s) 1905
PshBI ATTAAT 1 cut(s) 1710
Psp6I CCWGG 4 cut(s) 523, 541, 1145, 2173
PspEI GGTNACC 1 cut(s) 349
PspGI CCWGG 4 cut(s) 523, 541, 1145, 2173
PspN4I GGNNCC 2 cut(s) 649, 1730
PspPI GGNCC 3 cut(s) 399, 647, 707
PspXI VCTCGAGB 1 cut(s) 1722
PsuI RGATCY 4 cut(s) 1460, 1728, 1774, 2269
PsyI GACNNNGTC 1 cut(s) 2111
PvuII CAGCTG 1 cut(s) 2198
SaqAI TTAA 8 cut(s) 285, 693, 746, 848, 1152, 1470, 1710, 1812
SatI GCNGC 7 cut(s) 113, 126, 869, 1329, 1332, 1612, 2165
Sau3AI GATC 9 cut(s) 593, 613, 687, 1270, 1460, 1638, 1728, 1774, 2269
Sau96I GGNCC 3 cut(s) 399, 647, 707
ScaI AGTACT 1 cut(s) 1112
SchI GAGTC 4 cut(s) 482, 729, 1530, 2102
ScrFI CCNGG 6 cut(s) 360, 525, 543, 989, 1147, 2175
SduI GDGCHC 1 cut(s) 392
SexAI ACCWGGT 1 cut(s) 541
SfaNI GCATC 6 cut(s) 226, 595, 1598, 2046, 2083, 2105
SfcI CTRYAG 5 cut(s) 189, 802, 1401, 1957, 2199
Sfr274I CTCGAG 1 cut(s) 1722
SinI GGWCC 3 cut(s) 399, 647, 707
SlaI CTCGAG 1 cut(s) 1722
SmlI CTYRAG 3 cut(s) 621, 1169, 1722
SmoI CTYRAG 3 cut(s) 621, 1169, 1722
SpeI ACTAGT 1 cut(s) 2335
SphI GCATGC 1 cut(s) 2310
SsiI CCGC 4 cut(s) 204, 868, 1329, 2167
StyD4I CCNGG 6 cut(s) 358, 523, 541, 987, 1145, 2173
StyI CCWWGG 2 cut(s) 710, 895
TaiI ACGT 6 cut(s) 212, 258, 442, 770, 1101, 1956
TaqI TCGA 6 cut(s) 413, 686, 836, 1539, 1723, 2347
TaqII GACCGA 1 cut(s) 690
TatI WGTACW 5 cut(s) 158, 632, 798, 958, 1110
TauI GCSGC 2 cut(s) 871, 1331
TfiI GAWTC 7 cut(s) 427, 449, 1588, 1748, 2010, 2140, 2170
Tru1I TTAA 8 cut(s) 285, 693, 746, 848, 1152, 1470, 1710, 1812
Tru9I TTAA 8 cut(s) 285, 693, 746, 848, 1152, 1470, 1710, 1812
TscAI CASTG 5 cut(s) 38, 736, 1509, 1810, 2266
TseFI GTSAC 4 cut(s) 349, 935, 2113, 2323
TseI GCWGC 5 cut(s) 112, 125, 1331, 1611, 2164
Tsp45I GTSAC 4 cut(s) 349, 935, 2113, 2323
TspGWI ACGGA 4 cut(s) 22, 51, 1223, 2101
TspRI CASTG 5 cut(s) 38, 736, 1509, 1810, 2266
Tth111I GACNNNGTC 1 cut(s) 2111
Van91I CCANNNNNTGG 2 cut(s) 297, 716
VpaK11BI GGWCC 3 cut(s) 399, 647, 707
VspI ATTAAT 1 cut(s) 1710
XagI CCTNNNNNAGG 1 cut(s) 621
XapI RAATTY 4 cut(s) 363, 695, 1222, 1808
XbaI TCTAGA 2 cut(s) 1526, 1751
XceI RCATGY 6 cut(s) 972, 1909, 2051, 2077, 2194, 2310
XhoI CTCGAG 1 cut(s) 1722
XmnI GAANNNNTTC 1 cut(s) 1075
ZraI GACGTC 1 cut(s) 1954
ZrmI AGTACT 1 cut(s) 1112
Zsp2I ATGCAT 3 cut(s) 949, 2053, 2098
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.