MD10G1202800.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000484
Physical Location & Seq
Reverse (-)
30039754 .. 30043166
3413 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1202800.v1.1.491

Sequence Viewer

Length: 2502 bp
ATGAATTTAATGGAAAATCCATTGAAAGCTTCAACTAAGATGTGCTTGCTTCTTTTCTACTCTTCTTTGCTGCTCATCATCGCAGCGCTATTCTCTGCAGCCGATGACGCCACAAGCACATTTCAGTCCATTGCAGATGGTGAGACCATAGTCTCAGATGGTGGCACCTTTGAGCTTGGTTTTTTCAGTCCCGGTGCTTTGAAAAACCGCTACGTTGGGATATGGTACAAGAAGATATCTGTTACAACCATTGTATGGGTTGCCAACAGAGACACACCCGTCACTGATTCATCCGGCGTTCTGAAGGTCACCAACCCCGGACTTCTTGTCCTTCTCAGCCACAATATGAGCACGGTTTGGTCCTCCAACACATCGAGAACTGCACAGAATCCAGTGGCACGTCTTTTGGATTCGGGTAATCTTGTCGTCATAGATGGGAGTGATGTTGACCCTGAGAATTATCTGTGGCAAAGTTTTGACTACCCGGGCGATACATTCTTACCAGGTGCAAAGCTTGGTAGGAACACAGTTACAGGCTTCAATTGGCATTTTAGATCATGGAAAAGTCCTCAGGATCCTTCTCATGGAAATTATACATATCAACTTGGTCCCAAAGGATATGCAGAAAAATTTGTGAGGGAAGGTTCAGTCATAAAATTTCGGTCTGGACCATGGACTGGAGTCCATTTCCAGTTCAGTGGAACGCCTCAGTTAAATACAAACCCCATATACACATACAGTCTTGTTTCTGAGCCTGATGAAATTTACTATAGTTACAAGCTTCACAACAACTCTATCCTTTCGAGGGTGGTGTTAACTTCAGATGGAATTGTGCGGCGCTACACATGGATTGATAGAACCAAAGGTTGGCTGCTTCAACTAATAGCACAAATTGATAACTGTGACAACTATGCATTATGTGGTGTATATGGTGCATGTAACATTGAAAAGGCCCCGGTATGTAGCTGCTTGAAAGGATTTAAACCAAAGTATCCTAAAGAATGGGATTTGGTGGATTGGTCTAATGGCTGTGTGAGAAATGCTCCGCTGAATTGCAGTGGAGACGTGTTCATACAGTACTCGGGGATCAAATTGCCTAGCACAGAACAGTCCTGGCATAACAGAAGCATGAACCTCAAGGAATGTGAGATGGTGTGCAGAAAGAACTGCTCCTGCACAGCTTATACAAATTTGGATATCCGGGATGGAGGAAGCGGGTGCTTGATGTGGTACGGTGATCTGATTGACATAAGATACTTTGCTGAAAACGGGCAAGACATTTATATAAGAATGGCCGCTTCAGAACTAGATCATGAAGACGATACAAAGATCAACGCTCAATACTCTGAATCCAATGTGAAGAAAATGAGGATCATAGTAACCAGTATCGTGTTGTCTACTGGACTGCTGATCCTGGGCCTTGCCCTGCTCTATGTTTGGAAGAAGAAGTGTAAAAAAGATGGAAAACTGAGGTCCAACCAAAAGGAAGACCTAGAGTTACCATTATTTGACTTAAGTGATGTCGTTTGTGCTACCAGTGATTTTTCAAACCACAATAAGCTTGGAGAAGGTGGTTTCGGATCTGTCTTTAAGGGTACATTGAAAGATGGACAAGAAATAGCTGTGAAAAGGCTCTCCAAACATTCGAGACAAGGACTCAATGAGTTCAAGAATGAGGTTACACATATTGCCAAACTTCAGCACCGGAATCTAGTGAAGCTTCTAGGATGCTGCATTCAAGAAGACGAGATGATGCTGATCTACGAGTTCATGCCTAATAAAAGCTTAGACTTCTTTATTTTCGATCAAAGAAAAAGCATCTTACTAGATTGGCCTAAGCGTTTTGACATTATTAATGGGATTGCTCGGGGGCTCCTCTATCTTCATCAAGATTCTAGACTGAGAGTAATTCATCGAGATCTCAAAGGCGGTAACATTCTATTGGATGGTGAGTTTAATGCAAAAATTTCAGACTTTGGCCTGGCAAGAAGTTTTGGAGGAAATGAAACTTCGGCTAAGACGAAGAAAGTGGTTGGAACATATGGTTACATGTCCCCAGAATACGCCATCGATGGGTGCTACTCGATAAAATCCGATGTCTATAGCTTTGGTGTTATGGTGCTCGAGATAGTGAATGGGAAGAGGAACAGAGGATTTTCTCATCCAGGCCACAACCTCAACTTGCTAGGACATGCATGGAATTTATACACAGAAGGCATGTCTAATGAACTGCTTGATACATCAGTAGGAGACTCTAGTAATCAACATGAAGTTCTGCGATCAATCCATGTGGGTCTTTTATGTGTGCAACGAAATCCGGAAGATAGGCCAAGTATGCTAGCTGCAGTTCTGATGTTGAGTGGTGAAAGTGCATTGCCTCAACCTCAAAAACCTGGTTTTTATAGTGAAAGGGATCTAGATGACAACAATGTTGATCCTTGTGCAAACAAGGTTACATTTTCAGCTAATGAAGTCAGTTTTTCACTACTCGAAGCTCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

834

Amino Acids

93.29

Weight (kDa)

6.66

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 83 - 188 8.2e-38 D-mannose binding lectin
S_locus_glycop PF00954 220 - 330 6.8e-30 S-locus glycoprotein domain
PAN_2 PF08276 352 - 417 3.3e-20 PAN-like domain
Pkinase PF00069 516 - 718 2.6e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 518 - 780 2.1e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 278
AccB1I GGYRCC 1 cut(s) 164
AccB7I CCANNNNNTGG 3 cut(s) 255, 677, 867
AccI GTMKAC 1 cut(s) 1397
AccIII TCCGGA 1 cut(s) 2317
AciI CCGC 6 cut(s) 208, 835, 1046, 1215, 1296, 1929
AclWI GGATC 8 cut(s) 569, 582, 1094, 1379, 1405, 1588, 2421, 2429
AcoI YGGCCR 1 cut(s) 1293
AcsI RAATTY 7 cut(s) 4, 629, 656, 762, 1189, 1965, 2200
AcuI CTGAAG 4 cut(s) 323, 804, 1284, 1682
AcyI GRCGYC 1 cut(s) 108
AfaI GTAC 4 cut(s) 227, 1079, 1232, 1597
AfeI AGCGCT 1 cut(s) 87
AfiI CCNNNNNNNGG 6 cut(s) 255, 584, 677, 805, 867, 2073
AflII CTTAAG 1 cut(s) 1513
AflIII ACRYGT 2 cut(s) 1065, 2049
AhdI GACNNNNNGTC 1 cut(s) 326
AjiI CACGTC 2 cut(s) 401, 1066
AjnI CCWGG 6 cut(s) 502, 1112, 1413, 1980, 2164, 2392
AjuI GAANNNNNNNTTGG 2 cut(s) 850, 882
Alw21I GWGCWC 2 cut(s) 353, 2124
Alw26I GTCTC 6 cut(s) 137, 157, 264, 1056, 1642, 2244
AlwI GGATC 8 cut(s) 569, 582, 1094, 1379, 1405, 1588, 2421, 2429
Ama87I CYCGRG 4 cut(s) 484, 1081, 1866, 2123
Aor13HI TCCGGA 1 cut(s) 2317
Aor51HI AGCGCT 1 cut(s) 87
AoxI GGCC 7 cut(s) 951, 1293, 1417, 1832, 1978, 2167, 2327
ApeKI GCWGC 7 cut(s) 70, 83, 98, 871, 966, 1731, 2342
ApoI RAATTY 7 cut(s) 4, 629, 656, 762, 1189, 1965, 2200
AseI ATTAAT 1 cut(s) 1854
AspLEI GCGC 2 cut(s) 88, 840
AspS9I GGNCC 6 cut(s) 360, 608, 668, 952, 1417, 1473
AsuC2I CCSGG 6 cut(s) 192, 318, 485, 486, 956, 1202
AsuHPI GGTGA 5 cut(s) 152, 301, 1247, 1961, 2375
AsuNHI GCTAGC 1 cut(s) 2338
AvaI CYCGRG 4 cut(s) 484, 1081, 1866, 2123
AvaII GGWCC 4 cut(s) 360, 608, 668, 1473
AxyI CCTNAGG 1 cut(s) 570
BaeI ACNNNNGTAYC 4 cut(s) 974, 1007, 2229, 2262
BamHI GGATCC 1 cut(s) 574
BanI GGYRCC 1 cut(s) 164
BanII GRGCYC 1 cut(s) 1875
BbsI GAAGAC 3 cut(s) 1323, 1494, 1749
Bbv12I GWGCWC 2 cut(s) 353, 2124
BbvI GCAGC 7 cut(s) 57, 95, 110, 858, 953, 1718, 2329
BciT130I CCWGG 6 cut(s) 504, 1114, 1415, 1982, 2166, 2394
BciVI GTATCC 1 cut(s) 1002
BcnI CCSGG 6 cut(s) 192, 318, 485, 486, 956, 1202
BcoDI GTCTC 6 cut(s) 137, 157, 264, 1056, 1642, 2244
BfmI CTRYAG 4 cut(s) 96, 769, 2101, 2343
BfoI RGCGCY 2 cut(s) 89, 841
BfrI CTTAAG 1 cut(s) 1513
BfuI GTATCC 1 cut(s) 1002
BglII AGATCT 1 cut(s) 1918
BisI GCNGC 9 cut(s) 71, 84, 99, 836, 872, 967, 1296, 1732, 2343
BlsI GCNGC 9 cut(s) 72, 85, 100, 837, 873, 968, 1297, 1733, 2344
BmcAI AGTACT 1 cut(s) 1079
Bme18I GGWCC 4 cut(s) 360, 608, 668, 1473
BmeRI GACNNNNNGTC 1 cut(s) 326
BmeT110I CYCGRG 4 cut(s) 484, 1081, 1866, 2123
BmgBI CACGTC 2 cut(s) 401, 1066
BmgT120I GGNCC 6 cut(s) 360, 608, 668, 952, 1417, 1473
BmiI GGNNCC 5 cut(s) 166, 576, 610, 954, 1874
BmsI GCATC 3 cut(s) 1718, 1743, 1827
BmtI GCTAGC 1 cut(s) 2342
BoxI GACNNNNGTC 2 cut(s) 149, 680
BpiI GAAGAC 3 cut(s) 1323, 1494, 1749
BplI GAGNNNNNCTC 2 cut(s) 1027, 1059
BpmI CTGGAG 1 cut(s) 699
Bpu10I CCTNAGC 1 cut(s) 1836
BpuEI CTTGAG 1 cut(s) 1121
BpuMI CCSGG 6 cut(s) 192, 318, 485, 486, 956, 1202
Bsa29I ATCGAT 1 cut(s) 2070
BsaBI GATNNNNATC 1 cut(s) 1757
BsaHI GRCGYC 1 cut(s) 108
BsaI GGTCTC 1 cut(s) 137
BsaJI CCNNGG 5 cut(s) 316, 484, 671, 954, 1414
BsaWI WCCGGW 2 cut(s) 1704, 2317
Bsc4I CCNNNNNNNGG 6 cut(s) 255, 584, 677, 805, 867, 2073
Bse1I ACTGG 6 cut(s) 392, 682, 691, 1383, 1405, 1536
Bse21I CCTNAGG 1 cut(s) 570
Bse3DI GCAATG 2 cut(s) 129, 2372
Bse8I GATNNNNATC 1 cut(s) 1757
BseAI TCCGGA 1 cut(s) 2317
BseBI CCWGG 6 cut(s) 504, 1114, 1415, 1982, 2166, 2394
BseCI ATCGAT 1 cut(s) 2070
BseDI CCNNGG 5 cut(s) 316, 484, 671, 954, 1414
BseGI GGATG 5 cut(s) 290, 1210, 1733, 1951, 2161
BseJI GATNNNNATC 1 cut(s) 1757
BseLI CCNNNNNNNGG 6 cut(s) 255, 584, 677, 805, 867, 2073
BseMI GCAATG 2 cut(s) 129, 2372
BseMII CTCAG 8 cut(s) 168, 349, 444, 584, 722, 741, 1460, 1892
BseNI ACTGG 6 cut(s) 392, 682, 691, 1383, 1405, 1536
BseRI GAGGAG 1 cut(s) 1865
BseXI GCAGC 7 cut(s) 57, 95, 110, 858, 953, 1718, 2329
BsgI GTGCAG 3 cut(s) 366, 1159, 1177
BshFI GGCC 7 cut(s) 953, 1295, 1419, 1834, 1980, 2169, 2329
BshNI GGYRCC 1 cut(s) 164
BshVI ATCGAT 1 cut(s) 2070
BsiHKAI GWGCWC 2 cut(s) 353, 2124
BsiHKCI CYCGRG 4 cut(s) 484, 1081, 1866, 2123
BsiSI CCGG 8 cut(s) 192, 294, 318, 485, 956, 1201, 1705, 2318
BslFI GGGAC 3 cut(s) 174, 594, 2038
BslI CCNNNNNNNGG 6 cut(s) 255, 584, 677, 805, 867, 2073
BsmAI GTCTC 6 cut(s) 137, 157, 264, 1056, 1642, 2244
BsmBI CGTCTC 1 cut(s) 1056
BsmFI GGGAC 3 cut(s) 174, 594, 2038
BsmI GAATGC 1 cut(s) 1734
BsnI GGCC 7 cut(s) 953, 1295, 1419, 1834, 1980, 2169, 2329
Bso31I GGTCTC 1 cut(s) 137
BsoBI CYCGRG 4 cut(s) 484, 1081, 1866, 2123
Bsp1286I GDGCHC 3 cut(s) 353, 1875, 2124
Bsp13I TCCGGA 1 cut(s) 2317
Bsp19I CCATGG 1 cut(s) 671
BspACI CCGC 6 cut(s) 208, 835, 1046, 1215, 1296, 1929
BspANI GGCC 7 cut(s) 953, 1295, 1419, 1834, 1980, 2169, 2329
BspCNI CTCAG 8 cut(s) 167, 348, 445, 583, 721, 742, 1461, 1893
BspDI ATCGAT 1 cut(s) 2070
BspEI TCCGGA 1 cut(s) 2317
BspHI TCATGA 1 cut(s) 1312
BspLI GGNNCC 5 cut(s) 166, 576, 610, 954, 1874
BspMAI CTGCAG 2 cut(s) 100, 2347
BspOI GCTAGC 1 cut(s) 2342
BspPI GGATC 8 cut(s) 569, 582, 1094, 1379, 1405, 1588, 2421, 2429
BspT107I GGYRCC 1 cut(s) 164
BspTI CTTAAG 1 cut(s) 1513
BspTNI GGTCTC 1 cut(s) 137
BsrDI GCAATG 2 cut(s) 129, 2372
BsrI ACTGG 6 cut(s) 392, 682, 691, 1383, 1405, 1536
BssECI CCNNGG 5 cut(s) 316, 484, 671, 954, 1414
BssNI GRCGYC 1 cut(s) 108
BssT1I CCWWGG 1 cut(s) 671
Bst2UI CCWGG 6 cut(s) 504, 1114, 1415, 1982, 2166, 2394
Bst4CI ACNGT 7 cut(s) 355, 529, 740, 902, 1077, 1110, 1235
Bst6I CTCTTC 2 cut(s) 67, 2135
BstACI GRCGYC 1 cut(s) 108
BstAFI CTTAAG 1 cut(s) 1513
BstC8I GCNNGC 2 cut(s) 47, 2340
BstDSI CCRYGG 1 cut(s) 671
BstEII GGTNACC 1 cut(s) 307
BstF5I GGATG 5 cut(s) 290, 1210, 1733, 1951, 2161
BstH2I RGCGCY 2 cut(s) 89, 841
BstHHI GCGC 2 cut(s) 88, 840
BstMAI GTCTC 6 cut(s) 137, 157, 264, 1056, 1642, 2244
BstMWI GCNNNNNNNGC 2 cut(s) 107, 2335
BstNI CCWGG 6 cut(s) 504, 1114, 1415, 1982, 2166, 2394
BstNSI RCATGY 4 cut(s) 939, 2053, 2195, 2221
BstPAI GACNNNNGTC 2 cut(s) 149, 680
BstPI GGTNACC 1 cut(s) 307
BstSFI CTRYAG 4 cut(s) 96, 769, 2101, 2343
BstV1I GCAGC 7 cut(s) 57, 95, 110, 858, 953, 1718, 2329
BstV2I GAAGAC 3 cut(s) 1323, 1494, 1749
BstX2I RGATCY 4 cut(s) 574, 1580, 1918, 2413
BstXI CCANNNNNNTGG 1 cut(s) 698
BstYI RGATCY 4 cut(s) 574, 1580, 1918, 2413
Bsu15I ATCGAT 1 cut(s) 2070
Bsu36I CCTNAGG 1 cut(s) 570
BsuI GTATCC 1 cut(s) 1002
BsuRI GGCC 7 cut(s) 953, 1295, 1419, 1834, 1980, 2169, 2329
BsuTUI ATCGAT 1 cut(s) 2070
BtgI CCRYGG 1 cut(s) 671
BtgZI GCGATG 1 cut(s) 64
BtrI CACGTC 2 cut(s) 401, 1066
BtsCI GGATG 5 cut(s) 290, 1210, 1733, 1951, 2161
BtsI GCAGTG 1 cut(s) 1063
BtsIMutI CAGTG 5 cut(s) 282, 399, 703, 1063, 1543
Cac8I GCNNGC 2 cut(s) 47, 2340
CciI TCATGA 1 cut(s) 1312
CfoI GCGC 2 cut(s) 88, 840
Cfr13I GGNCC 6 cut(s) 360, 608, 668, 952, 1417, 1473
Cfr9I CCCGGG 1 cut(s) 484
ClaI ATCGAT 1 cut(s) 2070
CseI GACGC 1 cut(s) 116
CsiI ACCWGGT 2 cut(s) 502, 2392
Csp6I GTAC 4 cut(s) 226, 1078, 1231, 1596
CspCI CAANNNNNGTGG 2 cut(s) 2271, 2306
CviQI GTAC 4 cut(s) 226, 1078, 1231, 1596
DraI TTTAAA 1 cut(s) 982
DrdI GACNNNNNNGTC 1 cut(s) 278
DriI GACNNNNNGTC 1 cut(s) 326
DseDI GACNNNNNNGTC 1 cut(s) 278
EaeI YGGCCR 1 cut(s) 1293
Eam1104I CTCTTC 2 cut(s) 67, 2135
Eam1105I GACNNNNNGTC 1 cut(s) 326
EarI CTCTTC 2 cut(s) 67, 2135
Eco130I CCWWGG 1 cut(s) 671
Eco24I GRGCYC 1 cut(s) 1875
Eco31I GGTCTC 1 cut(s) 137
Eco32I GATATC 2 cut(s) 237, 1198
Eco47I GGWCC 4 cut(s) 360, 608, 668, 1473
Eco47III AGCGCT 1 cut(s) 87
Eco57I CTGAAG 4 cut(s) 323, 804, 1284, 1682
Eco81I CCTNAGG 1 cut(s) 570
Eco88I CYCGRG 4 cut(s) 484, 1081, 1866, 2123
Eco91I GGTNACC 1 cut(s) 307
EcoO109I RGGNCCY 1 cut(s) 952
EcoO65I GGTNACC 1 cut(s) 307
EcoRII CCWGG 6 cut(s) 502, 1112, 1413, 1980, 2164, 2392
EcoRV GATATC 2 cut(s) 237, 1198
EcoT14I CCWWGG 1 cut(s) 671
EcoT22I ATGCAT 2 cut(s) 916, 2197
EcoT38I GRGCYC 1 cut(s) 1875
ErhI CCWWGG 1 cut(s) 671
Esp3I CGTCTC 1 cut(s) 1056
FalI AAGNNNNNCTT 2 cut(s) 29, 61
FaqI GGGAC 3 cut(s) 174, 594, 2038
FauI CCCGC 1 cut(s) 1208
FauNDI CATATG 1 cut(s) 2041
FblI GTMKAC 1 cut(s) 1397
Fnu4HI GCNGC 9 cut(s) 71, 84, 99, 836, 872, 967, 1296, 1732, 2343
FokI GGATG 5 cut(s) 277, 1217, 1740, 1958, 2148
FriOI GRGCYC 1 cut(s) 1875
Fsp4HI GCNGC 9 cut(s) 71, 84, 99, 836, 872, 967, 1296, 1732, 2343
GlaI GCGC 2 cut(s) 87, 839
GluI GCNGC 9 cut(s) 71, 84, 99, 836, 872, 967, 1296, 1732, 2343
GsuI CTGGAG 1 cut(s) 699
HaeII RGCGCY 2 cut(s) 89, 841
HaeIII GGCC 7 cut(s) 953, 1295, 1419, 1834, 1980, 2169, 2329
HapII CCGG 8 cut(s) 192, 294, 318, 485, 956, 1201, 1705, 2318
HgaI GACGC 1 cut(s) 116
HhaI GCGC 2 cut(s) 88, 840
Hin1I GRCGYC 1 cut(s) 108
Hin6I GCGC 2 cut(s) 86, 838
HinP1I GCGC 2 cut(s) 86, 838
HincII GTYRAC 2 cut(s) 448, 816
HindII GTYRAC 2 cut(s) 448, 816
HindIII AAGCTT 6 cut(s) 27, 512, 779, 1559, 1718, 1783
HinfI GANTC 9 cut(s) 287, 388, 410, 681, 1349, 1656, 1708, 1892, 2252
HpaI GTTAAC 1 cut(s) 816
HpaII CCGG 8 cut(s) 192, 294, 318, 485, 956, 1201, 1705, 2318
HphI GGTGA 5 cut(s) 152, 301, 1247, 1961, 2375
Hpy166II GTNNAC 3 cut(s) 448, 816, 1398
Hpy8I GTNNAC 3 cut(s) 448, 816, 1398
HpyAV CCTTC 6 cut(s) 298, 341, 588, 635, 1562, 2207
HpyCH4III ACNGT 7 cut(s) 355, 529, 740, 902, 1077, 1110, 1235
HpyCH4IV ACGT 3 cut(s) 213, 400, 1065
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 2335
HpySE526I ACGT 3 cut(s) 213, 400, 1065
Hsp92I GRCGYC 1 cut(s) 108
HspAI GCGC 2 cut(s) 86, 838
Kpn2I TCCGGA 1 cut(s) 2317
KspAI GTTAAC 1 cut(s) 816
LmnI GCTCC 3 cut(s) 1048, 1175, 1878
Lsp1109I GCAGC 7 cut(s) 57, 95, 110, 858, 953, 1718, 2329
LweI GCATC 3 cut(s) 1718, 1743, 1827
MabI ACCWGGT 2 cut(s) 502, 2392
MaeII ACGT 3 cut(s) 213, 400, 1065
MfeI CAATTG 1 cut(s) 541
MflI RGATCY 4 cut(s) 574, 1580, 1918, 2413
MhlI GDGCHC 3 cut(s) 353, 1875, 2124
MlyI GAGTC 3 cut(s) 690, 1650, 2246
MmeI TCCRAC 3 cut(s) 390, 1500, 2014
Mph1103I ATGCAT 2 cut(s) 916, 2197
MroI TCCGGA 1 cut(s) 2317
MseI TTAA 8 cut(s) 8, 713, 815, 981, 1514, 1590, 1854, 1956
MspA1I CMGCKG 1 cut(s) 1048
MspCI CTTAAG 1 cut(s) 1513
MspI CCGG 8 cut(s) 192, 294, 318, 485, 956, 1201, 1705, 2318
MunI CAATTG 1 cut(s) 541
Mva1269I GAATGC 1 cut(s) 1734
MvaI CCWGG 6 cut(s) 504, 1114, 1415, 1982, 2166, 2394
MwoI GCNNNNNNNGC 2 cut(s) 107, 2335
NciI CCSGG 6 cut(s) 192, 318, 485, 486, 956, 1202
NcoI CCATGG 1 cut(s) 671
NdeI CATATG 1 cut(s) 2041
NheI GCTAGC 1 cut(s) 2338
NlaIV GGNNCC 5 cut(s) 166, 576, 610, 954, 1874
NmuCI GTSAC 3 cut(s) 280, 307, 902
NsiI ATGCAT 2 cut(s) 916, 2197
NspI RCATGY 4 cut(s) 939, 2053, 2195, 2221
PaeR7I CTCGAG 1 cut(s) 2123
PagI TCATGA 1 cut(s) 1312
PciI ACATGT 1 cut(s) 2049
PcsI WCGNNNNNNNCGW 1 cut(s) 2018
PctI GAATGC 1 cut(s) 1734
PfeI GAWTC 6 cut(s) 287, 388, 410, 1349, 1708, 1892
PflMI CCANNNNNTGG 3 cut(s) 255, 677, 867
PfoI TCCNGGA 1 cut(s) 1200
PkrI GCNGC 9 cut(s) 72, 85, 100, 837, 873, 968, 1297, 1733, 2344
PleI GAGTC 3 cut(s) 689, 1650, 2246
PpsI GAGTC 3 cut(s) 689, 1650, 2246
PscI ACATGT 1 cut(s) 2049
PshAI GACNNNNGTC 2 cut(s) 149, 680
PshBI ATTAAT 1 cut(s) 1854
Psp6I CCWGG 6 cut(s) 502, 1112, 1413, 1980, 2164, 2392
PspEI GGTNACC 1 cut(s) 307
PspGI CCWGG 6 cut(s) 502, 1112, 1413, 1980, 2164, 2392
PspN4I GGNNCC 5 cut(s) 166, 576, 610, 954, 1874
PspPI GGNCC 6 cut(s) 360, 608, 668, 952, 1417, 1473
PstI CTGCAG 2 cut(s) 100, 2347
PsuI RGATCY 4 cut(s) 574, 1580, 1918, 2413
RsaI GTAC 4 cut(s) 227, 1079, 1232, 1597
RsaNI GTAC 4 cut(s) 226, 1078, 1231, 1596
SaqAI TTAA 8 cut(s) 8, 713, 815, 981, 1514, 1590, 1854, 1956
SatI GCNGC 9 cut(s) 71, 84, 99, 836, 872, 967, 1296, 1732, 2343
Sau96I GGNCC 6 cut(s) 360, 608, 668, 952, 1417, 1473
ScaI AGTACT 1 cut(s) 1079
SchI GAGTC 3 cut(s) 690, 1650, 2246
SduI GDGCHC 3 cut(s) 353, 1875, 2124
SexAI ACCWGGT 2 cut(s) 502, 2392
SfaNI GCATC 3 cut(s) 1718, 1743, 1827
SfcI CTRYAG 4 cut(s) 96, 769, 2101, 2343
Sfr274I CTCGAG 1 cut(s) 2123
SinI GGWCC 4 cut(s) 360, 608, 668, 1473
SlaI CTCGAG 1 cut(s) 2123
SmaI CCCGGG 1 cut(s) 486
SmlI CTYRAG 3 cut(s) 1136, 1513, 2123
SmoI CTYRAG 3 cut(s) 1136, 1513, 2123
SsiI CCGC 6 cut(s) 208, 835, 1046, 1215, 1296, 1929
StyI CCWWGG 1 cut(s) 671
TaaI ACNGT 7 cut(s) 355, 529, 740, 902, 1077, 1110, 1235
TaiI ACGT 3 cut(s) 216, 403, 1068
TaqII GACCGA 1 cut(s) 651
TatI WGTACW 1 cut(s) 1077
TauI GCSGC 2 cut(s) 838, 1298
TfiI GAWTC 6 cut(s) 287, 388, 410, 1349, 1708, 1892
Tru1I TTAA 8 cut(s) 8, 713, 815, 981, 1514, 1590, 1854, 1956
Tru9I TTAA 8 cut(s) 8, 713, 815, 981, 1514, 1590, 1854, 1956
TscAI CASTG 5 cut(s) 289, 399, 703, 1063, 1543
TseFI GTSAC 3 cut(s) 280, 307, 902
TseI GCWGC 7 cut(s) 70, 83, 98, 871, 966, 1731, 2342
Tsp45I GTSAC 3 cut(s) 280, 307, 902
TspMI CCCGGG 1 cut(s) 484
TspRI CASTG 5 cut(s) 289, 399, 703, 1063, 1543
Van91I CCANNNNNTGG 3 cut(s) 255, 677, 867
Vha464I CTTAAG 1 cut(s) 1513
VpaK11BI GGWCC 4 cut(s) 360, 608, 668, 1473
VspI ATTAAT 1 cut(s) 1854
XapI RAATTY 7 cut(s) 4, 629, 656, 762, 1189, 1965, 2200
XbaI TCTAGA 2 cut(s) 1895, 2416
XceI RCATGY 4 cut(s) 939, 2053, 2195, 2221
XcmI CCANNNNNNNNNTGG 1 cut(s) 1559
XhoI CTCGAG 1 cut(s) 2123
XmaI CCCGGG 1 cut(s) 484
XmiI GTMKAC 1 cut(s) 1397
ZrmI AGTACT 1 cut(s) 1079
Zsp2I ATGCAT 2 cut(s) 916, 2197
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.