Rroxscaffold_1G00053000

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000484
Physical Location & Seq
Reverse (-)
73800287 .. 73805156
4870 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00053000.1

Sequence Viewer

Length: 2751 bp
ATGTGCTTACATCCTATTTGCTCTTTTCTACTGATCATCACAGCGATATTTTCCACAGCGGGAGGCAGCACAAGTACATTTCAGTCCATTAGAGATGGCCAGACTATAGTTTCAACTACTGGACACTTTGAGCTTGGATTTTTTAGGCCTTCTACTGGTTCTAATAATCGATATGTTGGGATATGGTACAAGAAGATATCGTCTACAATAGTATGGGTTGCCAACAGAGAAGCACCCCTTACTGATTCATCAGGTGTTCTGCAGGTGACCAACCCTGGAATTGTTGTCCTCCTCAACTATACCAAGAGCATAGTTTGGTCCTCAAACACATCAAGATCTGCAGAGAATCCAGTGGCCCAGCTTTTGGATTCAGGAAATCTTGTTGTAACAGATGGCAGTGATACAGACCCTGAGAACTTCTTGTGGCAAAGTTTTGATTACCCAAGTGATACATTCCTACCAGGTATGAAGCTTGGTTGGAACAGAGTTACAGGCTTCAATAGGCGTCTTACATCATGGAATAGTCCACAGGATCCTTCTCTAGGAAAATATACATTTGAACTTGATCGCAGAGGATATGGAGAAGAAATTTTGAAGAAGGAGTCAGTTATGATATGGCGAACTGGACCATGGAATGGAATCCGATTCAGTGGCTTGCCTCATTTAAGTCCAAACTCCGTATTCACATACAATCTTGTTTTAGATGATCATGATGAAGAATATTATATTTTTGAGCTTCGTAACAGCTCACTTTATTCAAGATTGACTCTCAATCCAGATGGGCGTGTGCAGCGCTACACATGGATTGATACAAACCAAACTTGGGGATTCCTTTATCAAACAGCTTTAATTGATAACTGCGAAAGTTATGCATTCTGTGGTCCAAATGGTGCATGCTGTATTGACAGTTTCCCAATATGTCGCTGCTTGAATGGATTTCAACCCAAATATCCAAAGGACTGGGGTGTAGTGGATTGGTCAAATGGCTGTGTGAGAAGGACTCCACTAAATTGCAGCGGTGACTTGTTTGAGAAGTACTCTGCAGTGAAATTGCCAAACACAGAGCAGTCCTGGTTCAACAGAAGTATGAATCTCAAGGAATGTGAGATGGTGTGCCTGAAGAACTGTTCCTGCACAGCTTATGCAAATTTGGATATCAAGGATGGAGGATCTGGCTGCTTGCTGTGGTTTGGCAGCCTAATTGATGTTAGAAATTTAACTGTAAATGGGCAAGATATTTATATAAGAATGGCTGCATCAGAACTAGGCCTGGATCATGACAACACAGTTATCAATGCTAAAGACTCTGAATCTAAGAAGGTTAGAATCATACTATGTGGTACTCTGTTGCCTACTGGACTGCTGATCGTGGGCATAGCCCTGGTATTTTATTGCCGGAAGAAGCAGAGCAAAAAATATGCTGAACCCCTCATCGCCTGGGAGAATGAGGAGCAGCAAAAAGAAGGTTCAGAACTTACCTCAGGAGAAGAAGGTTTAGAACTTCCCTCCAAAAAGGAAGATTTTGAACTTCCATTATTTGACTTGGCTACTGTCATTTGTGCCACTGATAACTTCTCAGAGAACAGAAAACTTGGACAAGGTGGATTCGGATCCGTCTTTAAGGGTATATTGCAAGATGGACAGGAAATAGCTGTGAAGAGGCTCTCGAAGCATTCTAAACAAGGACTGAATGAGTTCAAGAATGAGGTTACACATATTGTCAAACTTCAGCACAGGAATCTAGTGAAGCTCCTGGGATGCTGCATTCAGGAAGATGAAATGATGCTGATCTACGAATGCATGCCTAACAAAAGCTTGGACTTCTTTATTTTTGATCAAAGAAGAAGCTTCTTACTAAATTGGCCTAAGCGCTTTGACATTATTAATGGTATTGCTCGGGGGATCCTTTATCTTCATCAAGATTCTAGACTGAGAGTAATTCATAGAGACCTCAAAGCAGGCAATATCTTATTAGACGGTGAGTTCAACCCAAAAATCTCAGACTTTGGCCTGGCTAGAAGTTTTGGAGGAAATGAAACTAATGCAGAAACGAATAAAGTGGTCGGGACATATGGTTACATGTCCCCAGAATATGCAATTGACGGGTTCTACTCGATAAAATCAGACGTGTATAGCTTTGGTGTTTTGATGCTGGAGATAATAAGTGGGAAGAGAAACAGAGGATTCCTTCATCCAGGCCACAAGCTCAACCTGCTTGGACATGCATGGAACTTACACACAGAAGGCAGGTCCATTGAATTGCTTGCTACATCAGTAGGGGACTCCATTAATCTACAAGAAGTTCTGCGAACGATTCATGTGGGTCTTTTATGTGTTCAGCAAAATCCACAAGACCGGCCAAGCATGTCAGCTGCAGTACTAATGTTAAGTGGCGAAGGCCGGGGTGCTGGACTGTACCGCTTAAACCACGTTCAAGTGTTCAACATGAGGTGGCCCTCCAAAGATCCATGTGAAGCTCCTCATGTGTTTTATTTTGATTTTGTTGATCAGCGTGTTGAAGGAAACAAGACCGTTGCGTTTTATTCAAGAGTAGCTCCGCATGGTTTGCTGTCTTGTTCTTCAACCGATGGAGCCGACGGCGGAGAGTGGATCGGCCGGTGGCGGAGCGCATGGAGTGGTAGTCGCGCGGGAGGACCGGGGGACGTGGCCGGTGGGGAGGACCGCCACGCGGATCATGGAGCTGGTTTCGCTGCTCACGACCGCTCCATTTTGGATCGGAGTTTAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

916

Amino Acids

102.52

Weight (kDa)

6.2

Isoelectric Point (pI)

42.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 69 - 173 1.4e-39 D-mannose binding lectin
S_locus_glycop PF00954 206 - 316 8.1e-28 S-locus glycoprotein domain
PAN_2 PF08276 338 - 403 2.2e-20 PAN-like domain
Pkinase PF00069 526 - 725 1e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 528 - 791 2.1e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 253
Acc36I ACCTGC 3 cut(s) 253, 2220, 2238
AccB7I CCANNNNNTGG 2 cut(s) 364, 635
AccBSI CCGCTC 1 cut(s) 2724
AccI GTMKAC 1 cut(s) 203
AccII CGCG 3 cut(s) 2646, 2648, 2690
AcoI YGGCCR 4 cut(s) 97, 2357, 2614, 2667
AcsI RAATTY 3 cut(s) 588, 1147, 1213
AcuI CTGAAG 2 cut(s) 1139, 1712
AcyI GRCGYC 1 cut(s) 505
AfaI GTAC 6 cut(s) 76, 188, 1037, 1342, 2379, 2417
AfeI AGCGCT 2 cut(s) 794, 1871
AfiI CCNNNNNNNGG 6 cut(s) 155, 364, 542, 635, 823, 2689
AflIII ACRYGT 2 cut(s) 2079, 2127
AjiI CACGTC 2 cut(s) 2128, 2665
AjnI CCWGG 9 cut(s) 274, 460, 1070, 1269, 1380, 1436, 1752, 2010, 2194
Alw26I GTCTC 1 cut(s) 1941
AlwNI CAGNNNCTG 1 cut(s) 410
Ama87I CYCGRG 1 cut(s) 1896
Aor51HI AGCGCT 2 cut(s) 794, 1871
ApoI RAATTY 3 cut(s) 588, 1147, 1213
AseI ATTAAT 2 cut(s) 1884, 2289
Asp700I GAANNNNTTC 1 cut(s) 1694
AspLEI GCGC 4 cut(s) 795, 1872, 2630, 2648
AspS9I GGNCC 8 cut(s) 318, 355, 626, 881, 2250, 2454, 2654, 2680
AsuC2I CCSGG 2 cut(s) 2402, 2658
AsuHPI GGTGA 3 cut(s) 277, 1031, 1991
AvaI CYCGRG 1 cut(s) 1896
AvaII GGWCC 6 cut(s) 318, 626, 881, 2250, 2654, 2680
AxyI CCTNAGG 1 cut(s) 1480
BalI TGGCCA 1 cut(s) 99
BamHI GGATCC 3 cut(s) 532, 1610, 1902
BccI CCATC 7 cut(s) 89, 386, 773, 1102, 1157, 1631, 2582
BceAI ACGGC 1 cut(s) 2614
BcgI CGANNNNNNTGC 2 cut(s) 851, 885
BciT130I CCWGG 9 cut(s) 276, 462, 1072, 1271, 1382, 1438, 1754, 2012, 2196
BclI TGATCA 4 cut(s) 33, 706, 1834, 2506
BcnI CCSGG 2 cut(s) 2402, 2658
BcoDI GTCTC 1 cut(s) 1941
BfaI CTAG 5 cut(s) 542, 1265, 1742, 1926, 2016
BfmI CTRYAG 5 cut(s) 105, 260, 339, 1041, 2373
BfoI RGCGCY 2 cut(s) 796, 1873
BfuAI ACCTGC 3 cut(s) 253, 2220, 2238
BglII AGATCT 1 cut(s) 335
BmcAI AGTACT 2 cut(s) 1037, 2379
Bme18I GGWCC 6 cut(s) 318, 626, 881, 2250, 2654, 2680
BmeT110I CYCGRG 1 cut(s) 1896
BmgBI CACGTC 2 cut(s) 2128, 2665
BmgT120I GGNCC 8 cut(s) 318, 355, 626, 881, 2250, 2454, 2654, 2680
BmiI GGNNCC 4 cut(s) 534, 1612, 1904, 2593
BmrI ACTGGG 1 cut(s) 970
BmsI GCATC 4 cut(s) 1265, 1748, 1773, 2139
BmuI ACTGGG 1 cut(s) 970
BplI GAGNNNNNCTC 4 cut(s) 985, 1017, 1022, 1054
BpmI CTGGAG 1 cut(s) 2174
Bpu10I CCTNAGC 1 cut(s) 1866
BpuEI CTTGAG 1 cut(s) 1079
BpuMI CCSGG 2 cut(s) 2402, 2658
Bsa29I ATCGAT 1 cut(s) 169
BsaBI GATNNNNATC 2 cut(s) 1609, 1787
BsaHI GRCGYC 1 cut(s) 505
BsaI GGTCTC 1 cut(s) 1941
BsaJI CCNNGG 7 cut(s) 274, 629, 1380, 1437, 1753, 2401, 2657
BsaXI ACNNNNNCTCC 4 cut(s) 2626, 2656, 2708, 2738
Bsc4I CCNNNNNNNGG 6 cut(s) 155, 364, 542, 635, 823, 2689
Bse118I RCCGGY 3 cut(s) 2355, 2616, 2669
Bse1I ACTGG 6 cut(s) 124, 160, 350, 628, 965, 1360
Bse21I CCTNAGG 1 cut(s) 1480
Bse8I GATNNNNATC 2 cut(s) 1609, 1787
BseBI CCWGG 9 cut(s) 276, 462, 1072, 1271, 1382, 1438, 1754, 2012, 2196
BseCI ATCGAT 1 cut(s) 169
BseDI CCNNGG 7 cut(s) 274, 629, 1380, 1437, 1753, 2401, 2657
BseGI GGATG 4 cut(s) 10, 1168, 1763, 2191
BseJI GATNNNNATC 2 cut(s) 1609, 1787
BseLI CCNNNNNNNGG 6 cut(s) 155, 364, 542, 635, 823, 2689
BseMII CTCAG 5 cut(s) 402, 1494, 1590, 1922, 2013
BseNI ACTGG 6 cut(s) 124, 160, 350, 628, 965, 1360
BseRI GAGGAG 3 cut(s) 281, 1463, 2469
BseX3I CGGCCG 1 cut(s) 2614
BseYI CCCAGC 1 cut(s) 357
BsgI GTGCAG 2 cut(s) 809, 1117
Bsh1236I CGCG 3 cut(s) 2646, 2648, 2690
Bsh1285I CGRYCG 2 cut(s) 2617, 2722
BshVI ATCGAT 1 cut(s) 169
BsiEI CGRYCG 2 cut(s) 2617, 2722
BsiHKCI CYCGRG 1 cut(s) 1896
BsiSI CCGG 6 cut(s) 1396, 2356, 2401, 2617, 2657, 2670
BslFI GGGAC 4 cut(s) 2068, 2080, 2294, 2675
BslI CCNNNNNNNGG 6 cut(s) 155, 364, 542, 635, 823, 2689
BsmAI GTCTC 1 cut(s) 1941
BsmFI GGGAC 4 cut(s) 2068, 2080, 2294, 2675
BsmI GAATGC 4 cut(s) 872, 1672, 1764, 1802
Bso31I GGTCTC 1 cut(s) 1941
BsoBI CYCGRG 1 cut(s) 1896
Bsp19I CCATGG 1 cut(s) 629
BspCNI CTCAG 5 cut(s) 403, 1493, 1589, 1923, 2012
BspDI ATCGAT 1 cut(s) 169
BspFNI CGCG 3 cut(s) 2646, 2648, 2690
BspHI TCATGA 2 cut(s) 709, 1276
BspLI GGNNCC 4 cut(s) 534, 1612, 1904, 2593
BspMAI CTGCAG 4 cut(s) 264, 343, 1045, 2377
BspMI ACCTGC 3 cut(s) 253, 2220, 2238
BspTNI GGTCTC 1 cut(s) 1941
BsrBI CCGCTC 1 cut(s) 2724
BsrFI RCCGGY 3 cut(s) 2355, 2616, 2669
BsrI ACTGG 6 cut(s) 124, 160, 350, 628, 965, 1360
BssAI RCCGGY 3 cut(s) 2355, 2616, 2669
BssECI CCNNGG 7 cut(s) 274, 629, 1380, 1437, 1753, 2401, 2657
BssNI GRCGYC 1 cut(s) 505
BssT1I CCWWGG 1 cut(s) 629
Bst2UI CCWGG 9 cut(s) 276, 462, 1072, 1271, 1382, 1438, 1754, 2012, 2196
Bst4CI ACNGT 8 cut(s) 908, 1127, 1222, 1288, 1552, 1979, 2415, 2533
Bst6I CTCTTC 2 cut(s) 1652, 2165
BstACI GRCGYC 1 cut(s) 505
BstAPI GCANNNNNTGC 1 cut(s) 2566
BstC8I GCNNGC 6 cut(s) 656, 895, 1181, 1802, 1960, 2265
BstDEI CTNAG 7 cut(s) 411, 1314, 1480, 1576, 1866, 1931, 1999
BstDSI CCRYGG 1 cut(s) 629
BstEII GGTNACC 1 cut(s) 265
BstENI CCTNNNNNAGG 1 cut(s) 540
BstF5I GGATG 4 cut(s) 10, 1168, 1763, 2191
BstFNI CGCG 3 cut(s) 2646, 2648, 2690
BstH2I RGCGCY 2 cut(s) 796, 1873
BstHHI GCGC 4 cut(s) 795, 1872, 2630, 2648
BstMAI GTCTC 1 cut(s) 1941
BstMCI CGRYCG 2 cut(s) 2617, 2722
BstMWI GCNNNNNNNGC 5 cut(s) 790, 1669, 2212, 2566, 2708
BstNI CCWGG 9 cut(s) 276, 462, 1072, 1271, 1382, 1438, 1754, 2012, 2196
BstNSI RCATGY 5 cut(s) 897, 1804, 2083, 2225, 2368
BstPI GGTNACC 1 cut(s) 265
BstSFI CTRYAG 5 cut(s) 105, 260, 339, 1041, 2373
BstUI CGCG 3 cut(s) 2646, 2648, 2690
BstX2I RGATCY 6 cut(s) 335, 532, 1169, 1610, 1902, 2464
BstXI CCANNNNNNTGG 1 cut(s) 960
BstYI RGATCY 6 cut(s) 335, 532, 1169, 1610, 1902, 2464
BstZI CGGCCG 1 cut(s) 2614
Bsu15I ATCGAT 1 cut(s) 169
Bsu36I CCTNAGG 1 cut(s) 1480
BsuTUI ATCGAT 1 cut(s) 169
BtgI CCRYGG 1 cut(s) 629
BtgZI GCGATG 1 cut(s) 1417
BtrI CACGTC 2 cut(s) 2128, 2665
BtsCI GGATG 4 cut(s) 10, 1168, 1763, 2191
BtsI GCAGTG 2 cut(s) 403, 1050
BtsIMutI CAGTG 5 cut(s) 357, 403, 655, 1050, 1563
BveI ACCTGC 3 cut(s) 253, 2220, 2238
Cac8I GCNNGC 6 cut(s) 656, 895, 1181, 1802, 1960, 2265
CaiI CAGNNNCTG 1 cut(s) 410
CciI TCATGA 2 cut(s) 709, 1276
CfoI GCGC 4 cut(s) 795, 1872, 2630, 2648
Cfr10I RCCGGY 3 cut(s) 2355, 2616, 2669
Cfr13I GGNCC 8 cut(s) 318, 355, 626, 881, 2250, 2454, 2654, 2680
ClaI ATCGAT 1 cut(s) 169
CseI GACGC 1 cut(s) 494
CsiI ACCWGGT 1 cut(s) 460
Csp6I GTAC 6 cut(s) 75, 187, 1036, 1341, 2378, 2416
CviQI GTAC 6 cut(s) 75, 187, 1036, 1341, 2378, 2416
DdeI CTNAG 7 cut(s) 411, 1314, 1480, 1576, 1866, 1931, 1999
EaeI YGGCCR 4 cut(s) 97, 2357, 2614, 2667
EagI CGGCCG 1 cut(s) 2614
Eam1104I CTCTTC 2 cut(s) 1652, 2165
EarI CTCTTC 2 cut(s) 1652, 2165
EciI GGCGGA 2 cut(s) 2616, 2638
EclXI CGGCCG 1 cut(s) 2614
Eco130I CCWWGG 1 cut(s) 629
Eco147I AGGCCT 2 cut(s) 148, 1269
Eco31I GGTCTC 1 cut(s) 1941
Eco32I GATATC 2 cut(s) 198, 1156
Eco47I GGWCC 6 cut(s) 318, 626, 881, 2250, 2654, 2680
Eco47III AGCGCT 2 cut(s) 794, 1871
Eco52I CGGCCG 1 cut(s) 2614
Eco57I CTGAAG 2 cut(s) 1139, 1712
Eco81I CCTNAGG 1 cut(s) 1480
Eco88I CYCGRG 1 cut(s) 1896
Eco91I GGTNACC 1 cut(s) 265
EcoNI CCTNNNNNAGG 1 cut(s) 540
EcoO65I GGTNACC 1 cut(s) 265
EcoRII CCWGG 9 cut(s) 274, 460, 1070, 1269, 1380, 1436, 1752, 2010, 2194
EcoRV GATATC 2 cut(s) 198, 1156
EcoT14I CCWWGG 1 cut(s) 629
EcoT22I ATGCAT 3 cut(s) 874, 1802, 2227
ErhI CCWWGG 1 cut(s) 629
FalI AAGNNNNNCTT 2 cut(s) 222, 254
FaqI GGGAC 4 cut(s) 2068, 2080, 2294, 2675
FauI CCCGC 2 cut(s) 52, 2641
FauNDI CATATG 1 cut(s) 2071
FbaI TGATCA 4 cut(s) 33, 706, 1834, 2506
FblI GTMKAC 1 cut(s) 203
FokI GGATG 3 cut(s) 1175, 1770, 2178
FspBI CTAG 5 cut(s) 542, 1265, 1742, 1926, 2016
GlaI GCGC 4 cut(s) 794, 1871, 2629, 2647
GsaI CCCAGC 1 cut(s) 361
GsuI CTGGAG 1 cut(s) 2174
HaeII RGCGCY 2 cut(s) 796, 1873
HapII CCGG 6 cut(s) 1396, 2356, 2401, 2617, 2657, 2670
HgaI GACGC 1 cut(s) 494
HhaI GCGC 4 cut(s) 795, 1872, 2630, 2648
Hin1I GRCGYC 1 cut(s) 505
Hin6I GCGC 4 cut(s) 793, 1870, 2628, 2646
HinP1I GCGC 4 cut(s) 793, 1870, 2628, 2646
HindIII AAGCTT 3 cut(s) 470, 1813, 1846
HpaII CCGG 6 cut(s) 1396, 2356, 2401, 2617, 2657, 2670
HphI GGTGA 3 cut(s) 277, 1031, 1991
Hpy166II GTNNAC 2 cut(s) 204, 527
Hpy188I TCNGA 9 cut(s) 644, 1261, 1309, 1471, 1579, 1610, 2002, 2125, 2739
Hpy8I GTNNAC 2 cut(s) 204, 527
Hpy99I CGWCG 1 cut(s) 2600
HpyCH4III ACNGT 8 cut(s) 908, 1127, 1222, 1288, 1552, 1979, 2415, 2533
HpyCH4IV ACGT 3 cut(s) 2127, 2430, 2664
HpyF10VI GCNNNNNNNGC 5 cut(s) 790, 1669, 2212, 2566, 2708
HpyF3I CTNAG 7 cut(s) 411, 1314, 1480, 1576, 1866, 1931, 1999
HpySE526I ACGT 3 cut(s) 2127, 2430, 2664
Hsp92I GRCGYC 1 cut(s) 505
HspAI GCGC 4 cut(s) 793, 1870, 2628, 2646
Ksp22I TGATCA 4 cut(s) 33, 706, 1834, 2506
LmnI GCTCC 8 cut(s) 1450, 1755, 2482, 2560, 2591, 2625, 2699, 2729
LweI GCATC 4 cut(s) 1265, 1748, 1773, 2139
MabI ACCWGGT 1 cut(s) 460
MaeI CTAG 5 cut(s) 542, 1265, 1742, 1926, 2016
MaeII ACGT 3 cut(s) 2127, 2430, 2664
MaeIII GTNAC 7 cut(s) 265, 385, 487, 740, 1019, 1708, 2075
MbiI CCGCTC 1 cut(s) 2724
MfeI CAATTG 1 cut(s) 2097
MflI RGATCY 6 cut(s) 335, 532, 1169, 1610, 1902, 2464
MlsI TGGCCA 1 cut(s) 99
MluNI TGGCCA 1 cut(s) 99
MlyI GAGTC 5 cut(s) 611, 760, 994, 1298, 2276
MmeI TCCRAC 1 cut(s) 458
Mox20I TGGCCA 1 cut(s) 99
Mph1103I ATGCAT 3 cut(s) 874, 1802, 2227
MroXI GAANNNNTTC 1 cut(s) 1694
MscI TGGCCA 1 cut(s) 99
MseI TTAA 8 cut(s) 665, 848, 1217, 1620, 1884, 2289, 2387, 2423
Msp20I TGGCCA 1 cut(s) 99
MspA1I CMGCKG 3 cut(s) 59, 1017, 2372
MspI CCGG 6 cut(s) 1396, 2356, 2401, 2617, 2657, 2670
MunI CAATTG 1 cut(s) 2097
Mva1269I GAATGC 4 cut(s) 872, 1672, 1764, 1802
MvaI CCWGG 9 cut(s) 276, 462, 1072, 1271, 1382, 1438, 1754, 2012, 2196
MvnI CGCG 3 cut(s) 2646, 2648, 2690
MwoI GCNNNNNNNGC 5 cut(s) 790, 1669, 2212, 2566, 2708
NciI CCSGG 2 cut(s) 2402, 2658
NcoI CCATGG 1 cut(s) 629
NdeI CATATG 1 cut(s) 2071
NlaIV GGNNCC 4 cut(s) 534, 1612, 1904, 2593
NmuCI GTSAC 2 cut(s) 265, 1019
NsiI ATGCAT 3 cut(s) 874, 1802, 2227
NspI RCATGY 5 cut(s) 897, 1804, 2083, 2225, 2368
PaeI GCATGC 2 cut(s) 897, 1804
PagI TCATGA 2 cut(s) 709, 1276
PaqCI CACCTGC 1 cut(s) 253
PceI AGGCCT 2 cut(s) 148, 1269
PciI ACATGT 1 cut(s) 2079
PcsI WCGNNNNNNNCGW 1 cut(s) 2715
PctI GAATGC 4 cut(s) 872, 1672, 1764, 1802
PdmI GAANNNNTTC 1 cut(s) 1694
PflMI CCANNNNNTGG 2 cut(s) 364, 635
PleI GAGTC 5 cut(s) 610, 760, 994, 1298, 2276
PpsI GAGTC 5 cut(s) 610, 760, 994, 1298, 2276
PscI ACATGT 1 cut(s) 2079
PshBI ATTAAT 2 cut(s) 1884, 2289
Psp6I CCWGG 9 cut(s) 274, 460, 1070, 1269, 1380, 1436, 1752, 2010, 2194
PspEI GGTNACC 1 cut(s) 265
PspFI CCCAGC 1 cut(s) 357
PspGI CCWGG 9 cut(s) 274, 460, 1070, 1269, 1380, 1436, 1752, 2010, 2194
PspN4I GGNNCC 4 cut(s) 534, 1612, 1904, 2593
PspPI GGNCC 8 cut(s) 318, 355, 626, 881, 2250, 2454, 2654, 2680
PstI CTGCAG 4 cut(s) 264, 343, 1045, 2377
PstNI CAGNNNCTG 1 cut(s) 410
PsuI RGATCY 6 cut(s) 335, 532, 1169, 1610, 1902, 2464
PvuII CAGCTG 1 cut(s) 2372
RsaI GTAC 6 cut(s) 76, 188, 1037, 1342, 2379, 2417
RsaNI GTAC 6 cut(s) 75, 187, 1036, 1341, 2378, 2416
SaqAI TTAA 8 cut(s) 665, 848, 1217, 1620, 1884, 2289, 2387, 2423
Sau96I GGNCC 8 cut(s) 318, 355, 626, 881, 2250, 2454, 2654, 2680
ScaI AGTACT 2 cut(s) 1037, 2379
SchI GAGTC 5 cut(s) 611, 760, 994, 1298, 2276
SexAI ACCWGGT 1 cut(s) 460
SfaNI GCATC 4 cut(s) 1265, 1748, 1773, 2139
SfcI CTRYAG 5 cut(s) 105, 260, 339, 1041, 2373
SinI GGWCC 6 cut(s) 318, 626, 881, 2250, 2654, 2680
SmlI CTYRAG 1 cut(s) 1094
SmoI CTYRAG 1 cut(s) 1094
SphI GCATGC 2 cut(s) 897, 1804
SseBI AGGCCT 2 cut(s) 148, 1269
SspI AATATT 1 cut(s) 722
SspMI CTAG 5 cut(s) 542, 1265, 1742, 1926, 2016
StuI AGGCCT 2 cut(s) 148, 1269
StyI CCWWGG 1 cut(s) 629
TaaI ACNGT 8 cut(s) 908, 1127, 1222, 1288, 1552, 1979, 2415, 2533
TaiI ACGT 3 cut(s) 2130, 2433, 2667
TaqI TCGA 3 cut(s) 169, 1667, 2114
TatI WGTACW 3 cut(s) 74, 1035, 2377
Tru1I TTAA 8 cut(s) 665, 848, 1217, 1620, 1884, 2289, 2387, 2423
Tru9I TTAA 8 cut(s) 665, 848, 1217, 1620, 1884, 2289, 2387, 2423
TscAI CASTG 5 cut(s) 357, 403, 655, 1050, 1570
TseFI GTSAC 2 cut(s) 265, 1019
Tsp45I GTSAC 2 cut(s) 265, 1019
TspGWI ACGGA 2 cut(s) 667, 1603
TspRI CASTG 5 cut(s) 357, 403, 655, 1050, 1570
Van91I CCANNNNNTGG 2 cut(s) 364, 635
VpaK11BI GGWCC 6 cut(s) 318, 626, 881, 2250, 2654, 2680
VspI ATTAAT 2 cut(s) 1884, 2289
XagI CCTNNNNNAGG 1 cut(s) 540
XapI RAATTY 3 cut(s) 588, 1147, 1213
XbaI TCTAGA 1 cut(s) 1925
XceI RCATGY 5 cut(s) 897, 1804, 2083, 2225, 2368
XcmI CCANNNNNNNNNTGG 1 cut(s) 2693
XmiI GTMKAC 1 cut(s) 203
XmnI GAANNNNTTC 1 cut(s) 1694
XspI CTAG 5 cut(s) 542, 1265, 1742, 1926, 2016
ZrmI AGTACT 2 cut(s) 1037, 2379
Zsp2I ATGCAT 3 cut(s) 874, 1802, 2227
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.