Rh5AG185700

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000484
Physical Location & Seq
Forward (+)
21251276 .. 21256937
5662 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG185700.1

Sequence Viewer

Length: 519 bp
ATGACACTAGCTCCATTCCAAGAGGACCATTGTGGTGGGAAAGGATTACGCATTCCTAATTGCCATCCGAAGTTGAATGGCATTGGAAGTATCGATATTTTCCACAGTGGGAGGCAGCACAAAGGATATGGAGAAGAAATTTTGAAGAAGGATTCAGTTATGATGTGGCGAACTGGACCATGGAATGGAATCCGATTCAGTGGCACGCCTCATTTAACTCCAAACTCCATATTCACATACAATCTTGTTTTTGATGATCATGATGAAGAATATTATATTTTTGAGCTTCTTAACAGCTCAGTTTATTCAAGATTGATCCTCACTCCAGATGGGCGTGCTCTAAACATTAAAGGGCTTACAGCTGTATTAATCATCCTAAACCTTATAGCTCGAAGGGCATATCCTATTATCGTTGTACATTTCAAGATTAATCCTAACATCAATGTACATTTTGCAACTTTCCAACACAACTTCCTGGCCGTAGCTATGCGTCCCCCAATCACCCTCCTACCAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

172

Amino Acids

19.51

Weight (kDa)

8.95

Isoelectric Point (pI)

34.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S_locus_glycop PF00954 56 - 113 5.6e-09 S-locus glycoprotein domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000484)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G27290 AT4G27290
fragaria_vesca FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g15690 FvH4_3g21310 FvH4_3g21310
malus_domestica MD05G1219900.v1.1 MD05G1220200.v1.1 MD05G1230800.v1.1 MD05G1231100.v1.1 MD05G1231700.v1.1 MD05G1232100.v1.1 MD05G1232400.v1.1 MD05G1233000.v1.1 MD10G1202800.v1.1
prunus_persica Prupe.4G139500_v2.0.a1 Prupe.4G139500_v2.0.a1
pyrus_communis pycom05g20170 pycom05g20200 pycom05g20230 pycom05g20250 pycom05g20260 pycom05g20280 pycom05g20320 pycom05g20370 pycom05g20380 pycom05g24850 pycom10g17340
rosa_chinensis RchiOBHm_Chr5g0026351 RchiOBHm_Chr5g0026371 RchiOBHm_Chr5g0026381 RchiOBHm_Chr5g0026411 RchiOBHm_Chr5g0026551 RchiOBHm_Chr5g0036371
rosa_laevigata RLG00000032923 RLG00000032926 RLG00000032927 RLG00000032928 RLG00000032934 RLG00000033692
rosa_multiflora Rmu_co8389779.1_g000001 Rmu_sc0000712.1_g000012 Rmu_sc0001017.1_g000026 Rmu_sc0001114.1_g000001 Rmu_sc0001114.1_g000002 Rmu_sc0001114.1_g000008 Rmu_sc0005581.1_g000006 Rmu_sc0005581.1_g000007 Rmu_sc0006059.1_g000052 Rmu_sc0042966.1_g000001
rosa_roxburghii Rroxscaffold_1G00044440 Rroxscaffold_1G00052930 Rroxscaffold_1G00052980 Rroxscaffold_1G00052990 Rroxscaffold_1G00053000
rosa_rugosa Rorug05G0092500 Rorug05G0092500 Rorug05G0092500 Rorug05G0092600 Rorug05G0092700 Rorug05G0092700 Rorug05G0093700 Rorug05G0155200 Rorug05G0155800 Rorug05G0155800
rosa_samantha Rh5AG185700 Rh5AG186300 Rh5BG182600 Rh5BG182900 Rh5BG183600 Rh5BG249400 Rh5CG202000 Rh5CG202100 Rh5CG202200 Rh5CG203400 Rh5CG281200 Rh5DG184400 Rh5DG184500 Rh5DG185900
rosa_wichuraiana Rw5G016850 Rw5G016870 Rw5G016930 Rw5G022870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 185
AclWI GGATC 1 cut(s) 310
AcoI YGGCCR 1 cut(s) 477
AcsI RAATTY 1 cut(s) 138
AfaI GTAC 2 cut(s) 417, 447
AfiI CCNNNNNNNGG 1 cut(s) 185
AgsI TTSAA 4 cut(s) 76, 145, 309, 424
AjnI CCWGG 1 cut(s) 474
AluBI AGCT 6 cut(s) 11, 286, 297, 362, 389, 485
AluI AGCT 6 cut(s) 11, 286, 297, 362, 389, 485
Alw21I GWGCWC 1 cut(s) 340
AlwI GGATC 1 cut(s) 310
AoxI GGCC 1 cut(s) 477
ApeKI GCWGC 1 cut(s) 115
ApoI RAATTY 1 cut(s) 138
AseI ATTAAT 2 cut(s) 368, 429
AspS9I GGNCC 2 cut(s) 25, 176
AsuHPI GGTGA 1 cut(s) 493
AvaII GGWCC 2 cut(s) 25, 176
Bbv12I GWGCWC 1 cut(s) 340
BbvI GCAGC 1 cut(s) 127
BccI CCATC 2 cut(s) 72, 323
BceAI ACGGC 1 cut(s) 464
BciT130I CCWGG 1 cut(s) 476
BclI TGATCA 1 cut(s) 256
BfaI CTAG 1 cut(s) 8
BisI GCNGC 1 cut(s) 116
BlsI GCNGC 1 cut(s) 117
Bme1390I CCNGG 1 cut(s) 476
Bme18I GGWCC 2 cut(s) 25, 176
BmgT120I GGNCC 2 cut(s) 25, 176
BmrFI CCNGG 1 cut(s) 476
BpmI CTGGAG 1 cut(s) 309
Bsa29I ATCGAT 1 cut(s) 93
BsaJI CCNNGG 1 cut(s) 179
BsaXI ACNNNNNCTCC 1 cut(s) 25
Bsc4I CCNNNNNNNGG 1 cut(s) 185
Bse1I ACTGG 1 cut(s) 178
BseBI CCWGG 1 cut(s) 476
BseCI ATCGAT 1 cut(s) 93
BseDI CCNNGG 1 cut(s) 179
BseGI GGATG 2 cut(s) 64, 372
BseLI CCNNNNNNNGG 1 cut(s) 185
BseMII CTCAG 1 cut(s) 312
BseNI ACTGG 1 cut(s) 178
BseXI GCAGC 1 cut(s) 127
BshFI GGCC 1 cut(s) 479
BshVI ATCGAT 1 cut(s) 93
BsiHKAI GWGCWC 1 cut(s) 340
BslFI GGGAC 1 cut(s) 477
BslI CCNNNNNNNGG 1 cut(s) 185
BsmFI GGGAC 1 cut(s) 477
BsmI GAATGC 1 cut(s) 51
BsnI GGCC 1 cut(s) 479
Bsp1286I GDGCHC 1 cut(s) 340
Bsp1407I TGTACA 2 cut(s) 415, 445
Bsp143I GATC 2 cut(s) 256, 315
Bsp19I CCATGG 1 cut(s) 179
BspANI GGCC 1 cut(s) 479
BspCNI CTCAG 1 cut(s) 311
BspDI ATCGAT 1 cut(s) 93
BspHI TCATGA 1 cut(s) 259
BspPI GGATC 1 cut(s) 310
BsrGI TGTACA 2 cut(s) 415, 445
BsrI ACTGG 1 cut(s) 178
BssECI CCNNGG 1 cut(s) 179
BssMI GATC 2 cut(s) 256, 315
BssT1I CCWWGG 1 cut(s) 179
Bst2UI CCWGG 1 cut(s) 476
Bst4CI ACNGT 1 cut(s) 107
BstAUI TGTACA 2 cut(s) 415, 445
BstC8I GCNNGC 2 cut(s) 206, 336
BstDEI CTNAG 1 cut(s) 298
BstDSI CCRYGG 1 cut(s) 179
BstF5I GGATG 2 cut(s) 64, 372
BstKTI GATC 2 cut(s) 259, 318
BstMBI GATC 2 cut(s) 256, 315
BstMWI GCNNNNNNNGC 1 cut(s) 395
BstNI CCWGG 1 cut(s) 476
BstSCI CCNGG 1 cut(s) 474
BstV1I GCAGC 1 cut(s) 127
BstXI CCANNNNNNTGG 1 cut(s) 35
Bsu15I ATCGAT 1 cut(s) 93
BsuRI GGCC 1 cut(s) 479
BsuTUI ATCGAT 1 cut(s) 93
BtgI CCRYGG 1 cut(s) 179
BtsCI GGATG 2 cut(s) 64, 372
BtsIMutI CAGTG 2 cut(s) 112, 205
Cac8I GCNNGC 2 cut(s) 206, 336
CciI TCATGA 1 cut(s) 259
Cfr13I GGNCC 2 cut(s) 25, 176
ClaI ATCGAT 1 cut(s) 93
CseI GACGC 1 cut(s) 479
Csp6I GTAC 2 cut(s) 416, 446
CviAII CATG 2 cut(s) 180, 260
CviJI RGCY 8 cut(s) 11, 286, 297, 355, 362, 389, 479, 485
CviKI_1 RGCY 8 cut(s) 11, 286, 297, 355, 362, 389, 479, 485
CviQI GTAC 2 cut(s) 416, 446
DdeI CTNAG 1 cut(s) 298
DpnI GATC 2 cut(s) 258, 317
DpnII GATC 2 cut(s) 256, 315
EaeI YGGCCR 1 cut(s) 477
Eco130I CCWWGG 1 cut(s) 179
Eco47I GGWCC 2 cut(s) 25, 176
EcoRII CCWGG 1 cut(s) 474
EcoT14I CCWWGG 1 cut(s) 179
ErhI CCWWGG 1 cut(s) 179
FaeI CATG 2 cut(s) 183, 263
FaqI GGGAC 1 cut(s) 477
FatI CATG 2 cut(s) 179, 259
FbaI TGATCA 1 cut(s) 256
Fnu4HI GCNGC 1 cut(s) 116
FokI GGATG 2 cut(s) 51, 359
Fsp4HI GCNGC 1 cut(s) 116
FspBI CTAG 1 cut(s) 8
GluI GCNGC 1 cut(s) 116
GsuI CTGGAG 1 cut(s) 309
HaeIII GGCC 1 cut(s) 479
HgaI GACGC 1 cut(s) 479
Hin1II CATG 2 cut(s) 183, 263
HinfI GANTC 3 cut(s) 152, 189, 195
HphI GGTGA 1 cut(s) 493
Hpy188I TCNGA 2 cut(s) 69, 194
Hpy188III TCNNGA 4 cut(s) 260, 309, 326, 424
HpyAV CCTTC 2 cut(s) 142, 387
HpyCH4III ACNGT 1 cut(s) 107
HpyCH4V TGCA 1 cut(s) 455
HpyF10VI GCNNNNNNNGC 1 cut(s) 395
HpyF3I CTNAG 1 cut(s) 298
Hsp92II CATG 2 cut(s) 183, 263
Ksp22I TGATCA 1 cut(s) 256
Kzo9I GATC 2 cut(s) 256, 315
LmnI GCTCC 1 cut(s) 16
LpnPI CCDG 4 cut(s) 159, 339, 461, 488
Lsp1109I GCAGC 1 cut(s) 127
MaeI CTAG 1 cut(s) 8
MalI GATC 2 cut(s) 258, 317
MboI GATC 2 cut(s) 256, 315
MboII GAAGA 3 cut(s) 146, 157, 278
MhlI GDGCHC 1 cut(s) 340
MluCI AATT 3 cut(s) 58, 138, 513
MmeI TCCRAC 1 cut(s) 487
MnlI CCTC 5 cut(s) 16, 105, 219, 329, 515
MseI TTAA 6 cut(s) 215, 291, 348, 368, 429, 517
MslI CAYNNNNRTG 1 cut(s) 33
MspA1I CMGCKG 1 cut(s) 362
MspR9I CCNGG 1 cut(s) 476
Mva1269I GAATGC 1 cut(s) 51
MvaI CCWGG 1 cut(s) 476
MwoI GCNNNNNNNGC 1 cut(s) 395
NcoI CCATGG 1 cut(s) 179
NdeII GATC 2 cut(s) 256, 315
NlaIII CATG 2 cut(s) 183, 263
PagI TCATGA 1 cut(s) 259
PctI GAATGC 1 cut(s) 51
PfeI GAWTC 3 cut(s) 152, 189, 195
PflMI CCANNNNNTGG 1 cut(s) 185
PkrI GCNGC 1 cut(s) 117
PshBI ATTAAT 2 cut(s) 368, 429
Psp6I CCWGG 1 cut(s) 474
PspGI CCWGG 1 cut(s) 474
PspPI GGNCC 2 cut(s) 25, 176
PvuII CAGCTG 1 cut(s) 362
RsaI GTAC 2 cut(s) 417, 447
RsaNI GTAC 2 cut(s) 416, 446
RseI CAYNNNNRTG 1 cut(s) 33
SaqAI TTAA 6 cut(s) 215, 291, 348, 368, 429, 517
SatI GCNGC 1 cut(s) 116
Sau3AI GATC 2 cut(s) 256, 315
Sau96I GGNCC 2 cut(s) 25, 176
ScrFI CCNGG 1 cut(s) 476
SduI GDGCHC 1 cut(s) 340
SetI ASST 7 cut(s) 13, 288, 299, 364, 384, 391, 487
SinI GGWCC 2 cut(s) 25, 176
SmiMI CAYNNNNRTG 1 cut(s) 33
Sse9I AATT 3 cut(s) 58, 138, 513
SspI AATATT 1 cut(s) 272
SspMI CTAG 1 cut(s) 8
StyD4I CCNGG 1 cut(s) 474
StyI CCWWGG 1 cut(s) 179
TaaI ACNGT 1 cut(s) 107
TaqI TCGA 2 cut(s) 93, 391
TasI AATT 3 cut(s) 58, 138, 513
TatI WGTACW 2 cut(s) 415, 445
TfiI GAWTC 3 cut(s) 152, 189, 195
Tru1I TTAA 6 cut(s) 215, 291, 348, 368, 429, 517
Tru9I TTAA 6 cut(s) 215, 291, 348, 368, 429, 517
TscAI CASTG 2 cut(s) 112, 205
TseI GCWGC 1 cut(s) 115
TspDTI ATGAA 1 cut(s) 279
TspRI CASTG 2 cut(s) 112, 205
Van91I CCANNNNNTGG 1 cut(s) 185
VpaK11BI GGWCC 2 cut(s) 25, 176
VspI ATTAAT 2 cut(s) 368, 429
XapI RAATTY 1 cut(s) 138
XspI CTAG 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.